AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032933

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032933.t1 MTR_5g041400 87.637 364 42 2 1 362 1 363 0.0 621
MS.gene032933.t1 MTR_5g041380 86.253 371 42 2 1 362 1 371 0.0 621
MS.gene032933.t1 MTR_5g041530 61.364 396 104 13 1 362 1 381 1.05e-132 384
MS.gene032933.t1 MTR_5g041420 66.557 305 70 8 1 279 1 299 1.36e-110 326
MS.gene032933.t1 MTR_4g082060 36.555 476 167 16 4 361 6 464 5.76e-64 211
MS.gene032933.t1 MTR_7g010950 33.537 492 183 18 2 360 4 484 5.72e-60 201
MS.gene032933.t1 MTR_3g435480 39.106 358 127 15 90 360 110 463 9.17e-53 181
MS.gene032933.t1 MTR_6g012450 33.012 415 147 14 70 360 86 493 2.58e-47 167
MS.gene032933.t1 MTR_8g044220 31.858 452 191 22 4 362 12 439 1.77e-44 159
MS.gene032933.t1 MTR_7g086780 30.874 366 142 11 87 362 78 422 1.80e-34 131
MS.gene032933.t1 MTR_6g027460 58.889 90 31 2 88 171 60 149 1.94e-29 117
MS.gene032933.t1 MTR_2g016030 69.231 65 20 0 107 171 36 100 7.98e-29 110
MS.gene032933.t1 MTR_6g027450 53.333 90 36 2 88 171 53 142 4.96e-26 106
MS.gene032933.t1 MTR_8g079060 73.585 53 14 0 118 170 29 81 5.69e-23 96.3
MS.gene032933.t1 MTR_8g015840 71.154 52 15 0 118 169 20 71 1.79e-21 91.7
MS.gene032933.t1 MTR_4g109980 66.038 53 18 0 116 168 38 90 2.73e-21 93.6
MS.gene032933.t1 MTR_8g479350 61.905 63 24 0 118 180 68 130 2.94e-21 93.6
MS.gene032933.t1 MTR_4g022370 51.429 70 34 0 102 171 66 135 4.90e-21 93.6
MS.gene032933.t1 MTR_2g096740 66.667 54 18 0 118 171 62 115 5.61e-21 92.0
MS.gene032933.t1 MTR_2g096740 66.667 54 18 0 118 171 77 130 5.91e-21 92.0
MS.gene032933.t1 MTR_3g091820 61.667 60 23 0 115 174 40 99 7.22e-21 92.0
MS.gene032933.t1 MTR_2g059540 71.429 49 14 0 118 166 31 79 1.07e-20 89.4
MS.gene032933.t1 MTR_8g027295 56.250 64 28 0 108 171 14 77 1.07e-20 90.9
MS.gene032933.t1 MTR_2g093220 65.517 58 20 0 114 171 36 93 1.09e-20 91.3
MS.gene032933.t1 MTR_4g089095 63.636 55 20 0 118 172 50 104 1.77e-20 90.9
MS.gene032933.t1 MTR_2g014170 49.383 81 40 1 87 166 13 93 1.77e-20 90.9
MS.gene032933.t1 MTR_4g089095 63.636 55 20 0 118 172 16 70 2.11e-20 89.7
MS.gene032933.t1 MTR_4g088580 71.154 52 15 0 120 171 57 108 4.58e-20 90.9
MS.gene032933.t1 MTR_4g461080 61.538 65 21 1 110 170 17 81 4.66e-20 89.4
MS.gene032933.t1 MTR_1g077600 66.667 51 17 0 120 170 45 95 5.23e-20 89.0
MS.gene032933.t1 MTR_7g024670 68.627 51 16 0 120 170 79 129 5.28e-20 90.5
MS.gene032933.t1 MTR_1g077600 66.667 51 17 0 120 170 45 95 5.56e-20 89.4
MS.gene032933.t1 MTR_4g063780 44.660 103 56 1 102 203 59 161 1.13e-19 89.0
MS.gene032933.t1 MTR_7g059400 67.308 52 17 0 120 171 78 129 1.24e-19 89.4
MS.gene032933.t1 MTR_3g090430 62.712 59 18 1 113 171 50 104 2.11e-19 88.2
MS.gene032933.t1 MTR_8g068210 60.000 55 22 0 117 171 31 85 2.35e-19 88.2
MS.gene032933.t1 MTR_2g013370 69.231 52 15 1 120 171 48 98 2.42e-19 87.0
MS.gene032933.t1 MTR_1g056810 63.793 58 20 1 115 171 8 65 3.82e-19 87.0
MS.gene032933.t1 MTR_5g031440 60.000 55 22 0 114 168 39 93 1.70e-18 85.9
MS.gene032933.t1 MTR_2g014060 66.000 50 17 0 120 169 27 76 3.28e-18 85.1
MS.gene032933.t1 MTR_3g077750 66.667 51 17 0 118 168 61 111 4.45e-18 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032933.t1 AT5G62430 46.690 287 123 9 91 362 27 298 2.69e-62 201
MS.gene032933.t1 AT5G39660 37.366 372 145 15 74 360 87 455 3.37e-48 169
MS.gene032933.t1 AT5G39660 37.366 372 145 15 74 360 87 455 3.37e-48 169
MS.gene032933.t1 AT5G39660 37.366 372 145 15 74 360 87 455 3.37e-48 169
MS.gene032933.t1 AT1G69570 33.254 421 190 18 4 362 6 397 1.04e-46 164
MS.gene032933.t1 AT1G26790 31.783 387 210 11 4 362 12 372 1.44e-40 147
MS.gene032933.t1 AT1G29160 71.212 66 19 0 106 171 50 115 9.15e-32 118
MS.gene032933.t1 AT3G47500 61.616 99 37 1 77 174 68 166 4.55e-31 122
MS.gene032933.t1 AT3G47500 42.308 156 73 5 224 362 293 448 3.03e-20 92.0
MS.gene032933.t1 AT2G34140 52.577 97 40 1 81 171 15 111 2.73e-30 114
MS.gene032933.t1 AT3G55370 49.425 87 44 0 87 173 45 131 9.18e-23 97.8
MS.gene032933.t1 AT3G55370 49.425 87 44 0 87 173 90 176 9.90e-23 98.2
MS.gene032933.t1 AT3G55370 49.425 87 44 0 87 173 45 131 1.26e-22 97.8
MS.gene032933.t1 AT1G51700 64.062 64 23 0 118 181 33 96 1.43e-22 94.4
MS.gene032933.t1 AT5G60200 56.164 73 32 0 108 180 45 117 4.03e-22 94.7
MS.gene032933.t1 AT3G50410 73.077 52 14 0 118 169 30 81 7.77e-22 93.6
MS.gene032933.t1 AT5G60850 69.643 56 17 0 118 173 51 106 9.12e-22 94.7
MS.gene032933.t1 AT2G28810 41.237 97 57 0 73 169 32 128 1.53e-21 94.4
MS.gene032933.t1 AT2G28810 41.237 97 57 0 73 169 49 145 1.63e-21 94.4
MS.gene032933.t1 AT3G21270 69.811 53 16 0 118 170 29 81 5.74e-21 90.1
MS.gene032933.t1 AT5G66940 67.925 53 17 0 118 170 32 84 1.15e-20 89.7
MS.gene032933.t1 AT1G07640 58.209 67 25 1 104 170 10 73 1.55e-20 90.5
MS.gene032933.t1 AT1G07640 58.209 67 25 1 104 170 66 129 2.08e-20 91.3
MS.gene032933.t1 AT5G02460 55.882 68 30 0 104 171 81 148 2.23e-20 92.0
MS.gene032933.t1 AT1G07640 58.209 67 25 1 104 170 74 137 2.65e-20 90.9
MS.gene032933.t1 AT2G28510 55.714 70 31 0 108 177 38 107 2.82e-20 90.1
MS.gene032933.t1 AT2G37590 60.345 58 23 0 114 171 85 142 3.83e-20 90.5
MS.gene032933.t1 AT4G38000 59.375 64 25 1 113 175 36 99 3.85e-20 89.0
MS.gene032933.t1 AT3G45610 59.016 61 25 0 114 174 36 96 4.01e-20 89.0
MS.gene032933.t1 AT3G61850 45.263 95 47 1 114 208 58 147 4.22e-20 89.4
MS.gene032933.t1 AT3G61850 45.263 95 47 1 114 208 58 147 4.22e-20 89.4
MS.gene032933.t1 AT3G61850 60.656 61 24 0 114 174 70 130 5.00e-20 89.7
MS.gene032933.t1 AT1G28310 68.519 54 17 0 118 171 27 80 5.08e-20 89.7
MS.gene032933.t1 AT5G65590 57.353 68 26 1 106 173 32 96 5.54e-20 89.7
MS.gene032933.t1 AT3G61850 45.263 95 47 1 114 208 58 147 5.68e-20 89.7
MS.gene032933.t1 AT1G28310 68.519 54 17 0 118 171 51 104 5.89e-20 90.1
MS.gene032933.t1 AT3G61850 60.656 61 24 0 114 174 70 130 6.76e-20 89.7
MS.gene032933.t1 AT4G24060 61.290 62 20 1 110 171 49 106 9.22e-20 89.4
MS.gene032933.t1 AT1G47655 69.388 49 15 0 118 166 29 77 1.35e-19 86.7
MS.gene032933.t1 AT1G21340 50.685 73 36 0 97 169 17 89 1.86e-19 87.4
MS.gene032933.t1 AT1G64620 53.846 65 26 1 102 166 37 97 1.13e-18 86.7
MS.gene032933.t1 AT3G52440 63.462 52 19 0 120 171 27 78 1.50e-18 84.3
MS.gene032933.t1 AT4G00940 62.500 56 21 0 120 175 70 125 1.88e-18 85.1
MS.gene032933.t1 AT4G00940 62.500 56 21 0 120 175 70 125 1.88e-18 85.1
MS.gene032933.t1 AT4G00940 62.500 56 21 0 120 175 70 125 1.88e-18 85.1
MS.gene032933.t1 AT3G52440 63.462 52 19 0 120 171 46 97 2.14e-18 84.3
MS.gene032933.t1 AT4G21050 46.591 88 45 1 103 190 9 94 6.13e-18 82.0
MS.gene032933.t1 AT2G46590 57.627 59 24 1 108 165 57 115 6.96e-18 84.3
MS.gene032933.t1 AT2G46590 57.627 59 24 1 108 165 69 127 7.21e-18 84.3
MS.gene032933.t1 AT5G62940 67.347 49 16 0 120 168 75 123 9.90e-18 84.0
MS.gene032933.t1 AT4G21040 57.692 52 22 0 120 171 27 78 1.22e-15 75.9
MS.gene032933.t1 AT4G21080 57.692 52 22 0 120 171 27 78 4.30e-15 74.7

Find 71 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAATGGATTTGCATCTA+TGG 0.227266 5.4:+28178000 MS.gene032933:CDS
GTTCCAAGTAAACCTACTTT+AGG 0.229772 5.4:+28177827 MS.gene032933:CDS
TCTTCAATTCTTAATTTCTT+AGG 0.243331 5.4:-28177914 None:intergenic
GAACCCTTTGCAGCTTCATT+AGG 0.275268 5.4:-28177938 None:intergenic
GAGGTAGGTTCTTTCCTTGA+TGG 0.282122 5.4:-28177215 None:intergenic
ACTTTGTTCATCAACTTTCA+TGG 0.290874 5.4:-28177327 None:intergenic
AAGCTGCAAAGGGTTCTAAT+TGG 0.333760 5.4:+28177945 MS.gene032933:CDS
ATGAGTACTGTCTTGAACTT+TGG 0.343501 5.4:+28177584 MS.gene032933:CDS
TTCCAAGTAAACCTACTTTA+GGG 0.351236 5.4:+28177828 MS.gene032933:CDS
AACCCTTTGCAGCTTCATTA+GGG 0.351302 5.4:-28177937 None:intergenic
TTCCCTAAAGTAGGTTTACT+TGG 0.378626 5.4:-28177830 None:intergenic
AGTGAAGTTGAAGACATAAT+AGG 0.386239 5.4:-28177617 None:intergenic
AGCATACTATGGTGGTTGTT+TGG 0.399897 5.4:+28177763 MS.gene032933:CDS
TGTCAGAGATATTGGACTTC+CGG 0.405497 5.4:+28177473 MS.gene032933:CDS
GAATTCTTCAAAGAGATATA+AGG 0.418199 5.4:+28176634 MS.gene032933:CDS
CCTTGATAAAGCTGCAGGAT+TGG 0.440224 5.4:-28178071 None:intergenic
TTTGAAGAATTCACTTCAAG+TGG 0.441472 5.4:-28176623 None:intergenic
TTCAATGGATTTGCATCTAT+GGG 0.443602 5.4:+28178001 MS.gene032933:CDS
GAAACAGGATAACACATTGC+TGG 0.450520 5.4:-28177725 None:intergenic
ATGTTGGGTTGGACTGAAAC+AGG 0.462164 5.4:-28177740 None:intergenic
CTTGAATGTTTCCCTAAAGT+AGG 0.465584 5.4:-28177839 None:intergenic
CAACCCAACATAGCATACTA+TGG 0.468548 5.4:+28177752 MS.gene032933:CDS
ATCAATTTCAGAAACCATCA+AGG 0.479118 5.4:+28177201 MS.gene032933:intron
GGATCAGAAGGTGAAACATC+TGG 0.482490 5.4:+28177659 MS.gene032933:CDS
GCAGAAGTGACGGGGCTGGT+TGG 0.486295 5.4:-28177444 None:intergenic
TAACACATTGCTGGGTTCCA+TGG 0.491823 5.4:-28177716 None:intergenic
AAAATGAATGTTGGATCAGA+AGG 0.495845 5.4:+28177647 MS.gene032933:CDS
CCACCATAGTATGCTATGTT+GGG 0.509061 5.4:-28177755 None:intergenic
CATACTTCTAGTGGTTCCTC+CGG 0.515524 5.4:-28177492 None:intergenic
AGTTTCTGTTTCTGATGTGG+TGG 0.517750 5.4:-28177291 None:intergenic
AAAGACCTTGATAAAGCTGC+AGG 0.518898 5.4:-28178076 None:intergenic
CCAATCCTGCAGCTTTATCA+AGG 0.521791 5.4:+28178071 MS.gene032933:CDS
ATAGGTTGTACGCTCAATGA+AGG 0.524156 5.4:-28177788 None:intergenic
CTCGCCAAGAAAATGAATGT+TGG 0.525721 5.4:+28177638 MS.gene032933:CDS
AGAAGTTTCTGTTTCTGATG+TGG 0.528428 5.4:-28177294 None:intergenic
TTGACAATTATGTGAGGCAT+CGG 0.528875 5.4:-28177561 None:intergenic
AGAAGTATGCTTGTAGGTGC+AGG 0.531689 5.4:+28177506 MS.gene032933:CDS
TTAGGGAAACATTCAAGAGA+TGG 0.534355 5.4:+28177845 MS.gene032933:CDS
TTTGTTTGGAGCTTTGTCCT+GGG 0.536610 5.4:-28177357 None:intergenic
GACCCTAATGAAGCTGCAAA+GGG 0.538987 5.4:+28177935 MS.gene032933:CDS
ACCACTAGAAGTATGCTTGT+AGG 0.541265 5.4:+28177500 MS.gene032933:CDS
ACCACCATAGTATGCTATGT+TGG 0.544802 5.4:-28177756 None:intergenic
TCATAATCAAGTACTGAGGT+AGG 0.557713 5.4:-28177230 None:intergenic
CTAGGAAGCAAGAGTGGTGG+AGG 0.558467 5.4:+28177974 MS.gene032933:CDS
GTTTCTGTTTCTGATGTGGT+GGG 0.561237 5.4:-28177290 None:intergenic
GTGTTGAAATCCTCTGTGGT+GGG 0.563237 5.4:-28177266 None:intergenic
AAACAGGATAACACATTGCT+GGG 0.564718 5.4:-28177724 None:intergenic
AGACCCTAATGAAGCTGCAA+AGG 0.568759 5.4:+28177934 MS.gene032933:CDS
AGTGTTGAAATCCTCTGTGG+TGG 0.573158 5.4:-28177267 None:intergenic
GGTTCTAATTGGTTAACACT+AGG 0.578922 5.4:+28177956 MS.gene032933:CDS
GAGAAATCTTCATGAGCAAG+AGG 0.580785 5.4:-28176563 None:intergenic
TGAACAAAGTGATATTTCCC+AGG 0.582852 5.4:+28177340 MS.gene032933:CDS
CATAGTATGCTATGTTGGGT+TGG 0.584128 5.4:-28177751 None:intergenic
AAACTCAGAGGAACAATCAT+CGG 0.585405 5.4:-28176534 None:intergenic
GGGTTCCATGGAAACTGTTG+TGG 0.589831 5.4:-28177704 None:intergenic
TTCACAATCAAATCCTCTCA+CGG 0.599977 5.4:-28178105 None:intergenic
ACAGAAGATTGAGCTGCGAT+AGG 0.602850 5.4:-28177806 None:intergenic
ATTGGCTTTCAATACTGATG+AGG 0.608635 5.4:-28178053 None:intergenic
TAGGTTGTACGCTCAATGAA+GGG 0.629823 5.4:-28177787 None:intergenic
ACCTACAAGCATACTTCTAG+TGG 0.641003 5.4:-28177501 None:intergenic
AAATCTTCATGAGCAAGAGG+AGG 0.659290 5.4:-28176560 None:intergenic
ACACTAGGAAGCAAGAGTGG+TGG 0.671023 5.4:+28177971 MS.gene032933:CDS
ACTCATTTGACAATTATGTG+AGG 0.675408 5.4:-28177567 None:intergenic
TTAACACTAGGAAGCAAGAG+TGG 0.681260 5.4:+28177968 MS.gene032933:CDS
CCCAACATAGCATACTATGG+TGG 0.683395 5.4:+28177755 MS.gene032933:CDS
CAGAGATATTGGACTTCCGG+AGG 0.688190 5.4:+28177476 MS.gene032933:CDS
TTCTTCATAATCAAGTACTG+AGG 0.698198 5.4:-28177234 None:intergenic
GGGAGTGTTGAAATCCTCTG+TGG 0.719304 5.4:-28177270 None:intergenic
AAGAGGAGGAGAAAACTCAG+AGG 0.721417 5.4:-28176546 None:intergenic
GGGTGAAGATAAGAATCATG+TGG 0.723179 5.4:+28178021 MS.gene032933:CDS
AGCCTCAAAACCCACCACAG+AGG 0.733290 5.4:+28177256 MS.gene032933:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATATTAAACTAAAAGTT+AGG - chr5.4:28177075-28177094 None:intergenic 15.0%
!! AATAATTCACAATAAATCAT+AGG + chr5.4:28176862-28176881 MS.gene032933:intron 15.0%
!! GATTTATTGTGAATTATTAA+TGG - chr5.4:28176860-28176879 None:intergenic 15.0%
!!! AAATTTAATTACTTCTAGTT+AGG + chr5.4:28176692-28176711 MS.gene032933:intron 15.0%
!!! ACTTTGAGTTTATTTTTTAT+TGG + chr5.4:28177033-28177052 MS.gene032933:intron 15.0%
!!! ATGAAAAATATTTAACTTTG+TGG + chr5.4:28177142-28177161 MS.gene032933:intron 15.0%
!!! TGAAATATATACAATGTTTT+TGG + chr5.4:28176755-28176774 MS.gene032933:intron 15.0%
!! GAATAAAAAATAATATGAGC+TGG - chr5.4:28176962-28176981 None:intergenic 20.0%
!! TCTTCAATTCTTAATTTCTT+AGG - chr5.4:28177917-28177936 None:intergenic 20.0%
!! TGATAAACAATGATTAGATT+AGG - chr5.4:28177185-28177204 None:intergenic 20.0%
!! TTACAAGTAATTGTGATTAA+AGG + chr5.4:28176892-28176911 MS.gene032933:intron 20.0%
! GAATTCTTCAAAGAGATATA+AGG + chr5.4:28176634-28176653 MS.gene032933:CDS 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5.4:28176486-28176505 MS.gene032933:CDS 25.0%
AAAATGAATGTTGGATCAGA+AGG + chr5.4:28177647-28177666 MS.gene032933:CDS 30.0%
ACTCATTTGACAATTATGTG+AGG - chr5.4:28177570-28177589 None:intergenic 30.0%
ACTTTGTTCATCAACTTTCA+TGG - chr5.4:28177330-28177349 None:intergenic 30.0%
AGTGAAGTTGAAGACATAAT+AGG - chr5.4:28177620-28177639 None:intergenic 30.0%
ATCAATTTCAGAAACCATCA+AGG + chr5.4:28177201-28177220 MS.gene032933:intron 30.0%
TGAAGTCTATAATCCTAAAC+TGG + chr5.4:28176988-28177007 MS.gene032933:intron 30.0%
TTAAACTAAAAGTTAGGAGC+TGG - chr5.4:28177069-28177088 None:intergenic 30.0%
TTCCAAGTAAACCTACTTTA+GGG + chr5.4:28177828-28177847 MS.gene032933:CDS 30.0%
TTTAGAGAAAGACCAATAGT+AGG + chr5.4:28176936-28176955 MS.gene032933:intron 30.0%
TTTGAAGAATTCACTTCAAG+TGG - chr5.4:28176626-28176645 None:intergenic 30.0%
! AAGAAGAGATTTTGTTCTTG+CGG - chr5.4:28177535-28177554 None:intergenic 30.0%
! GATTCAACCATAAAGCTTTT+TGG + chr5.4:28176485-28176504 MS.gene032933:CDS 30.0%
! TAACTTTGTGGTTCATCTTT+TGG + chr5.4:28177154-28177173 MS.gene032933:intron 30.0%
! TGATCCAACATTCATTTTCT+TGG - chr5.4:28177645-28177664 None:intergenic 30.0%
! TTCAATGGATTTGCATCTAT+GGG + chr5.4:28178001-28178020 MS.gene032933:CDS 30.0%
! TTCTTCATAATCAAGTACTG+AGG - chr5.4:28177237-28177256 None:intergenic 30.0%
! TTTCAATGGATTTGCATCTA+TGG + chr5.4:28178000-28178019 MS.gene032933:CDS 30.0%
AAACAGGATAACACATTGCT+GGG - chr5.4:28177727-28177746 None:intergenic 35.0%
AAACTCAGAGGAACAATCAT+CGG - chr5.4:28176537-28176556 None:intergenic 35.0%
CTTGAATGTTTCCCTAAAGT+AGG - chr5.4:28177842-28177861 None:intergenic 35.0%
GCAAAAACTGTCAGAGATAT+TGG + chr5.4:28177465-28177484 MS.gene032933:CDS 35.0%
GTTCCAAGTAAACCTACTTT+AGG + chr5.4:28177827-28177846 MS.gene032933:CDS 35.0%
GTTGCAAATCAAACCAGTTT+AGG - chr5.4:28177004-28177023 None:intergenic 35.0%
TGAACAAAGTGATATTTCCC+AGG + chr5.4:28177340-28177359 MS.gene032933:CDS 35.0%
TTAGGGAAACATTCAAGAGA+TGG + chr5.4:28177845-28177864 MS.gene032933:CDS 35.0%
TTCCCTAAAGTAGGTTTACT+TGG - chr5.4:28177833-28177852 None:intergenic 35.0%
TTGACAATTATGTGAGGCAT+CGG - chr5.4:28177564-28177583 None:intergenic 35.0%
! ATGAGTACTGTCTTGAACTT+TGG + chr5.4:28177584-28177603 MS.gene032933:CDS 35.0%
! GGACATGGAAGAATTTTGTT+TGG - chr5.4:28177374-28177393 None:intergenic 35.0%
! GGTGTTGTGTGTTTGAAAAA+TGG - chr5.4:28176516-28176535 None:intergenic 35.0%
! GGTTCTAATTGGTTAACACT+AGG + chr5.4:28177956-28177975 MS.gene032933:CDS 35.0%
! TTTTTCCACAACAGTTTCCA+TGG + chr5.4:28177699-28177718 MS.gene032933:CDS 35.0%
!! AGAAGTTTCTGTTTCTGATG+TGG - chr5.4:28177297-28177316 None:intergenic 35.0%
!! ATTGGCTTTCAATACTGATG+AGG - chr5.4:28178056-28178075 None:intergenic 35.0%
!! TCATAATCAAGTACTGAGGT+AGG - chr5.4:28177233-28177252 None:intergenic 35.0%
AAAGACCTTGATAAAGCTGC+AGG - chr5.4:28178079-28178098 None:intergenic 40.0%
AAATCTTCATGAGCAAGAGG+AGG - chr5.4:28176563-28176582 None:intergenic 40.0%
AACCCTTTGCAGCTTCATTA+GGG - chr5.4:28177940-28177959 None:intergenic 40.0%
ACCACCATAGTATGCTATGT+TGG - chr5.4:28177759-28177778 None:intergenic 40.0%
ACCTACAAGCATACTTCTAG+TGG - chr5.4:28177504-28177523 None:intergenic 40.0%
AGCATACTATGGTGGTTGTT+TGG + chr5.4:28177763-28177782 MS.gene032933:CDS 40.0%
ATAGGTTGTACGCTCAATGA+AGG - chr5.4:28177791-28177810 None:intergenic 40.0%
CAACCCAACATAGCATACTA+TGG + chr5.4:28177752-28177771 MS.gene032933:CDS 40.0%
CATAGTATGCTATGTTGGGT+TGG - chr5.4:28177754-28177773 None:intergenic 40.0%
CCACCATAGTATGCTATGTT+GGG - chr5.4:28177758-28177777 None:intergenic 40.0%
CTCGCCAAGAAAATGAATGT+TGG + chr5.4:28177638-28177657 MS.gene032933:CDS 40.0%
GAAACAGGATAACACATTGC+TGG - chr5.4:28177728-28177747 None:intergenic 40.0%
GAGAAATCTTCATGAGCAAG+AGG - chr5.4:28176566-28176585 None:intergenic 40.0%
GGGTGAAGATAAGAATCATG+TGG + chr5.4:28178021-28178040 MS.gene032933:CDS 40.0%
GGTTCTCCCAAAAAGCTTTA+TGG - chr5.4:28176495-28176514 None:intergenic 40.0%
GTTAGGTGTATGCAATCTTG+TGG + chr5.4:28176709-28176728 MS.gene032933:intron 40.0%
TAGGTTGTACGCTCAATGAA+GGG - chr5.4:28177790-28177809 None:intergenic 40.0%
TGTCAGAGATATTGGACTTC+CGG + chr5.4:28177473-28177492 MS.gene032933:CDS 40.0%
TTAACACTAGGAAGCAAGAG+TGG + chr5.4:28177968-28177987 MS.gene032933:CDS 40.0%
! AAGCTGCAAAGGGTTCTAAT+TGG + chr5.4:28177945-28177964 MS.gene032933:CDS 40.0%
! AATATGAGCTGGCCTACTAT+TGG - chr5.4:28176951-28176970 None:intergenic 40.0%
! ACAGTTTTTGCAGAAGTGAC+GGG - chr5.4:28177456-28177475 None:intergenic 40.0%
! ACCACTAGAAGTATGCTTGT+AGG + chr5.4:28177500-28177519 MS.gene032933:CDS 40.0%
! GACAGTTTTTGCAGAAGTGA+CGG - chr5.4:28177457-28177476 None:intergenic 40.0%
! TTTGTTTGGAGCTTTGTCCT+GGG - chr5.4:28177360-28177379 None:intergenic 40.0%
!! AGTGGTGGAGGATTTTTCAA+TGG + chr5.4:28177986-28178005 MS.gene032933:CDS 40.0%
!! AGTTTCTGTTTCTGATGTGG+TGG - chr5.4:28177294-28177313 None:intergenic 40.0%
!! GTTTCTGTTTCTGATGTGGT+GGG - chr5.4:28177293-28177312 None:intergenic 40.0%
!! TTTTGTTTGGAGCTTTGTCC+TGG - chr5.4:28177361-28177380 None:intergenic 40.0%
AAGAGGAGGAGAAAACTCAG+AGG - chr5.4:28176549-28176568 None:intergenic 45.0%
ACAGAAGATTGAGCTGCGAT+AGG - chr5.4:28177809-28177828 None:intergenic 45.0%
AGACCCTAATGAAGCTGCAA+AGG + chr5.4:28177934-28177953 MS.gene032933:CDS 45.0%
CATACTTCTAGTGGTTCCTC+CGG - chr5.4:28177495-28177514 None:intergenic 45.0%
CCCAACATAGCATACTATGG+TGG + chr5.4:28177755-28177774 MS.gene032933:CDS 45.0%
CCTTGATAAAGCTGCAGGAT+TGG - chr5.4:28178074-28178093 None:intergenic 45.0%
GAACCCTTTGCAGCTTCATT+AGG - chr5.4:28177941-28177960 None:intergenic 45.0%
GACCCTAATGAAGCTGCAAA+GGG + chr5.4:28177935-28177954 MS.gene032933:CDS 45.0%
GAGGTAGGTTCTTTCCTTGA+TGG - chr5.4:28177218-28177237 None:intergenic 45.0%
GGATCAGAAGGTGAAACATC+TGG + chr5.4:28177659-28177678 MS.gene032933:CDS 45.0%
GTGTTGAAATCCTCTGTGGT+GGG - chr5.4:28177269-28177288 None:intergenic 45.0%
TAACACATTGCTGGGTTCCA+TGG - chr5.4:28177719-28177738 None:intergenic 45.0%
! ACGCTTTTGCATCTTGGACA+TGG - chr5.4:28177389-28177408 None:intergenic 45.0%
! AGAAGTATGCTTGTAGGTGC+AGG + chr5.4:28177506-28177525 MS.gene032933:CDS 45.0%
! AGTGTTGAAATCCTCTGTGG+TGG - chr5.4:28177270-28177289 None:intergenic 45.0%
! CAGTTTTTGCAGAAGTGACG+GGG - chr5.4:28177455-28177474 None:intergenic 45.0%
! CCAATCCTGCAGCTTTATCA+AGG + chr5.4:28178071-28178090 MS.gene032933:CDS 45.0%
! GGTCTTTTATGTTCCGTGAG+AGG + chr5.4:28178092-28178111 MS.gene032933:CDS 45.0%
! GTGTCTACGCTTTTGCATCT+TGG - chr5.4:28177395-28177414 None:intergenic 45.0%
!! ATGTTGGGTTGGACTGAAAC+AGG - chr5.4:28177743-28177762 None:intergenic 45.0%
ACACTAGGAAGCAAGAGTGG+TGG + chr5.4:28177971-28177990 MS.gene032933:CDS 50.0%
CAGAGATATTGGACTTCCGG+AGG + chr5.4:28177476-28177495 MS.gene032933:CDS 50.0%
GGGTTCCATGGAAACTGTTG+TGG - chr5.4:28177707-28177726 None:intergenic 50.0%
!! GGGAGTGTTGAAATCCTCTG+TGG - chr5.4:28177273-28177292 None:intergenic 50.0%
!!! ATCCTCTGTGGTGGGTTTTG+AGG - chr5.4:28177261-28177280 None:intergenic 50.0%
AGCCTCAAAACCCACCACAG+AGG + chr5.4:28177256-28177275 MS.gene032933:CDS 55.0%
CTAGGAAGCAAGAGTGGTGG+AGG + chr5.4:28177974-28177993 MS.gene032933:CDS 55.0%
!! TTTTGCAGAAGTGACGGGGC+TGG - chr5.4:28177451-28177470 None:intergenic 55.0%
! GCAGAAGTGACGGGGCTGGT+TGG - chr5.4:28177447-28177466 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 28176476 28178120 28176476 ID=MS.gene032933
chr5.4 mRNA 28176476 28178120 28176476 ID=MS.gene032933.t1;Parent=MS.gene032933
chr5.4 exon 28176476 28176655 28176476 ID=MS.gene032933.t1.exon1;Parent=MS.gene032933.t1
chr5.4 CDS 28176476 28176655 28176476 ID=cds.MS.gene032933.t1;Parent=MS.gene032933.t1
chr5.4 exon 28177212 28178120 28177212 ID=MS.gene032933.t1.exon2;Parent=MS.gene032933.t1
chr5.4 CDS 28177212 28178120 28177212 ID=cds.MS.gene032933.t1;Parent=MS.gene032933.t1
Gene Sequence

>MS.gene032933

ATGACTAATGATTCAACCATAAAGCTTTTTGGGAGAACCATTTTTCAAACACACAACACCGATGATTGTTCCTCTGAGTTTTCTCCTCCTCTTGCTCATGAAGATTTCTCTGATCATAGTTTTCATTCATCTTTATCTTCGTCTTCTCCACTTGAAGTGAATTCTTCAAAGAGATATAAGGTATGTTTCATGCAAATTTTTCATCTTTTTTGTTTTAAATTTAATTACTTCTAGTTAGGTGTATGCAATCTTGTGGCTGTTTTGACAACTTTTTTTGCTTGAAATATATACAATGTTTTTGGATTTCATGATGACAGATGCTTGATGAAATTGTGCACTTTTTTCATTTGTATATTTATTTAAAAGTTTAGTTTTGTAAAACCATTAATAATTCACAATAAATCATAGGTACAACTTTACAAGTAATTGTGATTAAAGGAACTTCATCAAAGTTTCATTCTTTAGAGAAAGACCAATAGTAGGCCAGCTCATATTATTTTTTATTCATATTTTGAAGTCTATAATCCTAAACTGGTTTGATTTGCAACTTCAACTCAACTTTGAGTTTATTTTTTATTGGATCTCATAGACCAGCTCCTAACTTTTAGTTTAATATCTTAATGAATCAGTTAATCTATACTTTCAATTCATGAAAAATTCATGAAAATGAAAAATATTTAACTTTGTGGTTCATCTTTTGGTCTTACCTAATCTAATCATTGTTTATCAATTTCAGAAACCATCAAGGAAAGAACCTACCTCAGTACTTGATTATGAAGAAGCCTCAAAACCCACCACAGAGGATTTCAACACTCCCACCACATCAGAAACAGAAACTTCTCAACTGAATTCCATGAAAGTTGATGAACAAAGTGATATTTCCCAGGACAAAGCTCCAAACAAAATTCTTCCATGTCCAAGATGCAAAAGCGTAGACACAAAATTTTGTTACTACAATAATTACAACGCCAACCAGCCCCGTCACTTCTGCAAAAACTGTCAGAGATATTGGACTTCCGGAGGAACCACTAGAAGTATGCTTGTAGGTGCAGGTCGCCGCAAGAACAAAATCTCTTCTTTTTCTTCCGATGCCTCACATAATTGTCAAATGAGTACTGTCTTGAACTTTGGATCAGATTCTCCTATTATGTCTTCAACTTCACTCGCCAAGAAAATGAATGTTGGATCAGAAGGTGAAACATCTGGTAAGAGTAATCAGTGTTTTTTTCCACAACAGTTTCCATGGAACCCAGCAATGTGTTATCCTGTTTCAGTCCAACCCAACATAGCATACTATGGTGGTTGTTTGGTCCCTTCATTGAGCGTACAACCTATCGCAGCTCAATCTTCTGTTCCAAGTAAACCTACTTTAGGGAAACATTCAAGAGATGGCTTAGAAAATAACAAGAAAGAAAGTGATAATAATAGTGTGTTGATTCCTAAGAAATTAAGAATTGAAGACCCTAATGAAGCTGCAAAGGGTTCTAATTGGTTAACACTAGGAAGCAAGAGTGGTGGAGGATTTTTCAATGGATTTGCATCTATGGGTGAAGATAAGAATCATGTGGTTGAAGCAGCCTCATCAGTATTGAAAGCCAATCCTGCAGCTTTATCAAGGTCTTTTATGTTCCGTGAGAGGATTTGA
Protein sequence

>MS.gene032933.t1

MTNDSTIKLFGRTIFQTHNTDDCSSEFSPPLAHEDFSDHSFHSSLSSSSPLEVNSSKRYKKPSRKEPTSVLDYEEASKPTTEDFNTPTTSETETSQLNSMKVDEQSDISQDKAPNKILPCPRCKSVDTKFCYYNNYNANQPRHFCKNCQRYWTSGGTTRSMLVGAGRRKNKISSFSSDASHNCQMSTVLNFGSDSPIMSSTSLAKKMNVGSEGETSGKSNQCFFPQQFPWNPAMCYPVSVQPNIAYYGGCLVPSLSVQPIAAQSSVPSKPTLGKHSRDGLENNKKESDNNSVLIPKKLRIEDPNEAAKGSNWLTLGSKSGGGFFNGFASMGEDKNHVVEAASSVLKANPAALSRSFMFRERI