AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene032937

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032937.t1 MTR_5g041530 88.601 386 39 1 1 386 1 381 0.0 650
MS.gene032937.t1 MTR_5g041380 63.131 396 111 9 1 386 1 371 7.59e-154 438
MS.gene032937.t1 MTR_5g041400 61.675 394 112 9 1 386 1 363 2.15e-148 424
MS.gene032937.t1 MTR_5g041420 72.787 305 71 4 1 297 1 301 1.42e-141 405
MS.gene032937.t1 MTR_4g082060 42.029 483 147 19 7 384 9 463 5.98e-91 281
MS.gene032937.t1 MTR_7g010950 36.970 495 186 15 2 384 4 484 2.54e-70 229
MS.gene032937.t1 MTR_6g012450 37.292 421 147 13 80 384 74 493 1.20e-64 214
MS.gene032937.t1 MTR_3g435480 36.496 411 153 14 69 384 66 463 3.08e-59 199
MS.gene032937.t1 MTR_8g044220 32.133 361 142 10 115 386 93 439 1.73e-47 167
MS.gene032937.t1 MTR_6g027460 55.140 107 45 1 98 201 51 157 8.97e-33 126
MS.gene032937.t1 MTR_6g027450 53.846 104 45 1 101 201 47 150 1.11e-30 120
MS.gene032937.t1 MTR_7g086780 42.138 159 90 2 78 235 36 193 2.21e-30 120
MS.gene032937.t1 MTR_2g016030 77.966 59 13 0 135 193 42 100 2.05e-29 112
MS.gene032937.t1 MTR_8g015840 66.667 63 17 1 133 191 9 71 3.96e-24 99.4
MS.gene032937.t1 MTR_2g096740 58.571 70 29 0 124 193 46 115 5.26e-23 98.2
MS.gene032937.t1 MTR_2g096740 58.571 70 29 0 124 193 61 130 6.07e-23 98.2
MS.gene032937.t1 MTR_4g022370 56.522 69 30 0 125 193 67 135 1.16e-22 98.6
MS.gene032937.t1 MTR_8g479350 68.966 58 17 1 142 198 70 127 3.71e-22 96.7
MS.gene032937.t1 MTR_3g091820 58.209 67 28 0 127 193 30 96 4.53e-22 95.9
MS.gene032937.t1 MTR_8g079060 53.846 78 26 1 125 192 4 81 4.81e-22 94.0
MS.gene032937.t1 MTR_8g068210 52.703 74 34 1 122 194 14 87 6.62e-22 95.9
MS.gene032937.t1 MTR_7g024670 46.078 102 42 3 105 205 54 143 6.76e-22 96.3
MS.gene032937.t1 MTR_4g109980 69.231 52 16 0 139 190 39 90 1.25e-21 94.7
MS.gene032937.t1 MTR_3g090430 58.904 73 28 2 127 197 36 108 1.39e-21 94.7
MS.gene032937.t1 MTR_8g027295 62.712 59 22 0 135 193 19 77 1.63e-21 93.6
MS.gene032937.t1 MTR_2g059540 68.627 51 16 0 138 188 29 79 1.90e-21 91.7
MS.gene032937.t1 MTR_2g093220 60.606 66 25 1 129 193 28 93 2.80e-21 93.2
MS.gene032937.t1 MTR_1g077600 55.072 69 31 0 127 195 30 98 3.57e-21 92.4
MS.gene032937.t1 MTR_1g077600 55.072 69 31 0 127 195 30 98 4.48e-21 92.8
MS.gene032937.t1 MTR_4g063780 63.934 61 21 1 136 195 71 131 4.66e-21 93.6
MS.gene032937.t1 MTR_4g088580 71.154 52 15 0 142 193 57 108 2.21e-20 92.0
MS.gene032937.t1 MTR_7g059400 60.656 61 23 1 133 193 70 129 4.85e-20 90.9
MS.gene032937.t1 MTR_4g461080 60.000 65 25 1 129 192 17 81 4.87e-20 89.4
MS.gene032937.t1 MTR_5g031440 63.636 55 20 0 136 190 39 93 7.38e-20 90.1
MS.gene032937.t1 MTR_1g056810 67.308 52 17 0 142 193 14 65 9.16e-20 89.0
MS.gene032937.t1 MTR_4g089095 62.264 53 20 0 142 194 52 104 1.92e-19 88.2
MS.gene032937.t1 MTR_4g089095 62.264 53 20 0 142 194 18 70 2.04e-19 87.4
MS.gene032937.t1 MTR_2g013370 68.519 54 16 1 140 193 46 98 3.18e-19 87.0
MS.gene032937.t1 MTR_2g014060 66.000 50 17 0 142 191 27 76 1.06e-18 86.7
MS.gene032937.t1 MTR_3g077750 62.963 54 20 0 137 190 58 111 1.38e-18 86.3
MS.gene032937.t1 MTR_2g014170 67.347 49 16 0 140 188 45 93 1.41e-18 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene032937.t1 AT5G62430 44.828 290 132 10 109 386 25 298 2.50e-64 207
MS.gene032937.t1 AT5G39660 37.852 391 158 14 70 384 74 455 6.31e-58 196
MS.gene032937.t1 AT5G39660 37.852 391 158 14 70 384 74 455 6.31e-58 196
MS.gene032937.t1 AT5G39660 37.852 391 158 14 70 384 74 455 6.31e-58 196
MS.gene032937.t1 AT1G69570 37.092 337 145 17 98 386 80 397 9.53e-47 164
MS.gene032937.t1 AT1G26790 36.042 283 134 10 135 386 106 372 4.85e-38 140
MS.gene032937.t1 AT1G29160 73.913 69 15 1 125 193 50 115 1.63e-32 120
MS.gene032937.t1 AT2G34140 57.143 91 27 2 125 206 46 133 1.02e-30 115
MS.gene032937.t1 AT3G47500 63.415 82 30 0 122 203 92 173 4.95e-30 120
MS.gene032937.t1 AT3G47500 42.945 163 70 6 240 386 293 448 1.08e-23 102
MS.gene032937.t1 AT1G51700 73.077 52 14 0 140 191 33 84 5.35e-23 95.9
MS.gene032937.t1 AT1G07640 54.545 77 29 1 123 199 10 80 6.85e-23 97.4
MS.gene032937.t1 AT5G60200 45.098 102 39 1 92 193 24 108 8.04e-23 97.1
MS.gene032937.t1 AT1G07640 54.545 77 29 1 123 199 66 136 9.17e-23 98.2
MS.gene032937.t1 AT1G07640 54.545 77 29 1 123 199 74 144 1.09e-22 98.2
MS.gene032937.t1 AT2G37590 54.412 68 31 0 126 193 75 142 2.27e-22 97.1
MS.gene032937.t1 AT5G60850 70.370 54 16 0 140 193 51 104 2.86e-22 96.3
MS.gene032937.t1 AT3G21270 74.510 51 13 0 142 192 31 81 4.45e-22 93.6
MS.gene032937.t1 AT2G28810 48.810 84 40 2 108 191 48 128 4.87e-22 95.9
MS.gene032937.t1 AT2G28810 48.810 84 40 2 108 191 65 145 6.58e-22 95.9
MS.gene032937.t1 AT3G61850 38.017 121 72 1 73 193 10 127 8.16e-22 95.1
MS.gene032937.t1 AT3G61850 38.017 121 72 1 73 193 10 127 1.26e-21 94.7
MS.gene032937.t1 AT3G55370 60.317 63 24 1 131 193 68 129 1.66e-21 94.7
MS.gene032937.t1 AT3G55370 60.317 63 24 1 131 193 68 129 2.03e-21 94.7
MS.gene032937.t1 AT3G55370 60.317 63 24 1 131 193 113 174 2.05e-21 95.1
MS.gene032937.t1 AT4G24060 60.000 65 26 0 129 193 42 106 2.41e-21 94.4
MS.gene032937.t1 AT3G50410 69.231 52 16 0 140 191 30 81 3.40e-21 92.4
MS.gene032937.t1 AT4G38000 51.948 77 36 1 139 214 40 116 4.39e-21 91.7
MS.gene032937.t1 AT3G61850 57.576 66 28 0 128 193 50 115 6.66e-21 92.0
MS.gene032937.t1 AT3G61850 57.576 66 28 0 128 193 50 115 6.66e-21 92.0
MS.gene032937.t1 AT3G45610 43.564 101 55 1 133 231 33 133 9.25e-21 90.9
MS.gene032937.t1 AT3G61850 57.576 66 28 0 128 193 50 115 9.89e-21 92.0
MS.gene032937.t1 AT5G65590 53.750 80 29 2 119 193 18 94 1.21e-20 92.0
MS.gene032937.t1 AT3G52440 48.315 89 45 1 137 224 22 110 1.85e-20 90.1
MS.gene032937.t1 AT3G52440 50.000 86 42 1 137 221 41 126 3.10e-20 89.7
MS.gene032937.t1 AT5G02460 62.069 58 22 0 136 193 91 148 3.54e-20 91.7
MS.gene032937.t1 AT1G28310 66.667 54 18 0 140 193 27 80 5.73e-20 90.1
MS.gene032937.t1 AT2G28510 67.308 52 17 0 142 193 50 101 5.89e-20 89.4
MS.gene032937.t1 AT1G28310 66.667 54 18 0 140 193 51 104 8.66e-20 89.7
MS.gene032937.t1 AT5G66940 66.667 51 17 0 142 192 34 84 1.09e-19 87.4
MS.gene032937.t1 AT1G21340 59.677 62 24 1 138 198 35 96 1.88e-19 87.4
MS.gene032937.t1 AT1G64620 63.462 52 19 0 137 188 46 97 2.13e-19 89.0
MS.gene032937.t1 AT4G00940 55.556 72 27 1 127 193 50 121 2.18e-19 88.2
MS.gene032937.t1 AT4G00940 55.556 72 27 1 127 193 50 121 2.18e-19 88.2
MS.gene032937.t1 AT4G00940 55.556 72 27 1 127 193 50 121 2.18e-19 88.2
MS.gene032937.t1 AT1G47655 65.306 49 17 0 140 188 29 77 4.52e-19 85.1
MS.gene032937.t1 AT4G21050 67.308 52 17 0 142 193 26 77 1.03e-18 84.3
MS.gene032937.t1 AT2G46590 60.377 53 21 0 135 187 63 115 6.47e-18 84.7
MS.gene032937.t1 AT2G46590 60.377 53 21 0 135 187 75 127 6.79e-18 84.7
MS.gene032937.t1 AT5G62940 67.347 49 16 0 142 190 75 123 7.50e-18 84.7
MS.gene032937.t1 AT4G21040 61.538 52 20 0 142 193 27 78 9.78e-17 79.3
MS.gene032937.t1 AT4G21080 61.538 52 20 0 142 193 27 78 3.91e-16 77.8

Find 71 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACTCTTTGCAATTTCATT+AGG 0.246474 5.4:-28250176 None:intergenic
GGTCAAAATTCAACTACATT+AGG 0.249796 5.4:+28250038 MS.gene032937:CDS
ACCGCCATAGTATGCTATAT+TGG 0.313531 5.4:-28249967 None:intergenic
TCATCGATTCTTAGTGTCTT+AGG 0.320046 5.4:-28250152 None:intergenic
ACAATTATGTCCGGTTTCAT+TGG 0.357374 5.4:-28249591 None:intergenic
AACTCTTTGCAATTTCATTA+GGG 0.357550 5.4:-28250175 None:intergenic
GTCAAAATTCAACTACATTA+GGG 0.362039 5.4:+28250039 MS.gene032937:CDS
TGCCAGAGGTATTGGACTTC+CGG 0.368367 5.4:+28249702 MS.gene032937:CDS
TTAGGGAAACATTCAAGAAA+TGG 0.383950 5.4:+28250056 MS.gene032937:CDS
TCGGTTGGAGAATTCACTTC+AGG 0.387221 5.4:-28248557 None:intergenic
CCGCCATAGTATGCTATATT+GGG 0.397382 5.4:-28249966 None:intergenic
TATCCCAATATAGCATACTA+TGG 0.401203 5.4:+28249963 MS.gene032937:CDS
AGAAGCAAACTCTAACTCTA+AGG 0.408119 5.4:-28248477 None:intergenic
AGTACTACTATCTTGACCTT+TGG 0.413423 5.4:+28249798 MS.gene032937:CDS
AGCATACTATGGCGGTTGTT+TGG 0.417812 5.4:+28249974 MS.gene032937:CDS
GGGGAACATGAAGATGAATA+AGG 0.417962 5.4:-28248533 None:intergenic
CAGTTTAGAAGTTTCTGTTT+CGG 0.422590 5.4:-28249520 None:intergenic
AAATGATGGAAAGAGATATA+AGG 0.429316 5.4:+28248580 MS.gene032937:CDS
CATATTTCTCGTTGCTCCTC+CGG 0.432490 5.4:-28249721 None:intergenic
TAACTCTAAGGAAGAATCAT+TGG 0.432927 5.4:-28248465 None:intergenic
GACACAGGATAACACATTGC+AGG 0.443245 5.4:-28249936 None:intergenic
CAACACTAGGAATCAAGAAC+GGG 0.446060 5.4:+28250207 MS.gene032937:CDS
GAGGAATCTTCATGAGGAAA+AGG 0.451641 5.4:-28248500 None:intergenic
GGGTTCTCAACAAGGCTTGA+AGG 0.457419 5.4:-28249483 None:intergenic
TAGGGAAACATTCAAGAAAT+GGG 0.472410 5.4:+28250057 MS.gene032937:CDS
GCAACGAGAAATATGATTGT+AGG 0.472417 5.4:+28249729 MS.gene032937:CDS
TTCATGGGATTCGCATCCAA+AGG 0.488816 5.4:+28250239 MS.gene032937:CDS
AGAGAAATCTCCAATGAAAC+CGG 0.492605 5.4:+28249581 MS.gene032937:CDS
GGACAGGGAACAATTATGTC+CGG 0.498365 5.4:-28249600 None:intergenic
CTCCGGAAGTCCAATACCTC+TGG 0.499281 5.4:-28249704 None:intergenic
TCATGGGATTCGCATCCAAA+GGG 0.501709 5.4:+28250240 MS.gene032937:CDS
CTTGACAAGAAAGTGAATGT+TGG 0.504154 5.4:+28249852 MS.gene032937:CDS
GCGGTTGTTTGGTACCATCA+TGG 0.511292 5.4:+28249985 MS.gene032937:CDS
TTCGCAATCAAATCCTCTCA+TGG 0.511946 5.4:-28250340 None:intergenic
AATTCTCCAACCGAAAATGA+TGG 0.512006 5.4:+28248566 MS.gene032937:CDS
AAATTGCAAAGAGTTCTATA+TGG 0.517606 5.4:+28250183 MS.gene032937:CDS
AGAAGTTTCTGTTTCGGACG+AGG 0.520692 5.4:-28249514 None:intergenic
CTAATCCTGTAGCTTTATCA+AGG 0.525653 5.4:+28250306 MS.gene032937:CDS
AGAAATATGATTGTAGGTGC+AGG 0.532367 5.4:+28249735 MS.gene032937:CDS
TTGTGTTGCAGGAAACATCA+TGG 0.539505 5.4:+28249391 MS.gene032937:intron
GAAGTTTCTGTTTCGGACGA+GGG 0.541471 5.4:-28249513 None:intergenic
AGAGACCTTGATAAAGCTAC+AGG 0.544478 5.4:-28250311 None:intergenic
AACAAACTCAATGCAGCCAA+TGG 0.545923 5.4:+28249768 MS.gene032937:CDS
GGTCTCTTGTGTTCCATGAG+AGG 0.551572 5.4:+28250327 MS.gene032937:CDS
CATATCAGTTCAAGAGGATG+AGG 0.559879 5.4:+28249422 MS.gene032937:CDS
AGTAGTACTATGCAATCCAT+TGG 0.564693 5.4:-28249784 None:intergenic
GTTGGAGAATTCACTTCAGG+TGG 0.567909 5.4:-28248554 None:intergenic
AGAACTCATATCAGTTCAAG+AGG 0.588411 5.4:+28249416 MS.gene032937:CDS
AGTAGAAGAATCTGATCCAA+AGG 0.598047 5.4:-28249814 None:intergenic
TTGGAGAATTCACTTCAGGT+GGG 0.604331 5.4:-28248553 None:intergenic
CTGTCCAAGATGCAAAAGCA+TGG 0.610924 5.4:+28249617 MS.gene032937:CDS
ATAGATTGTACATTCCATGA+TGG 0.621316 5.4:-28249999 None:intergenic
AGTTCTATATGGTCAACACT+AGG 0.622239 5.4:+28250194 MS.gene032937:CDS
ACACAGGATAACACATTGCA+GGG 0.622856 5.4:-28249935 None:intergenic
TCAACACTAGGAATCAAGAA+CGG 0.637810 5.4:+28250206 MS.gene032937:CDS
ATGGGATTCGCATCCAAAGG+GGG 0.645601 5.4:+28250242 MS.gene032937:CDS
TGAATAAGGACTATGATCAG+AGG 0.646385 5.4:-28248519 None:intergenic
ATCCTCTGTAGATTGGAATG+AGG 0.648122 5.4:-28249445 None:intergenic
TGGAGAATTCACTTCAGGTG+GGG 0.648645 5.4:-28248552 None:intergenic
TCTATCTCGACTCAATCTTG+TGG 0.657132 5.4:+28250017 MS.gene032937:CDS
ATATTGGGATAGAATGACAC+AGG 0.661691 5.4:-28249951 None:intergenic
CCCAATATAGCATACTATGG+CGG 0.676610 5.4:+28249966 MS.gene032937:CDS
GGGTTCCAAGAAAACTGTGG+AGG 0.676664 5.4:-28249915 None:intergenic
ATTAGCTTGCAAAACTGACG+AGG 0.678686 5.4:-28250288 None:intergenic
TGATCAGAGGAATCTTCATG+AGG 0.678996 5.4:-28248506 None:intergenic
CAGAGGTATTGGACTTCCGG+AGG 0.686773 5.4:+28249705 MS.gene032937:CDS
CATGGGATTCGCATCCAAAG+GGG 0.694560 5.4:+28250241 MS.gene032937:CDS
AACACTAGGAATCAAGAACG+GGG 0.710662 5.4:+28250208 MS.gene032937:CDS
GGCCTCATTCCAATCTACAG+AGG 0.711504 5.4:+28249443 MS.gene032937:CDS
GCAGGGTTCCAAGAAAACTG+TGG 0.717555 5.4:-28249918 None:intergenic
ACACTAGGAATCAAGAACGG+GGG 0.749858 5.4:+28250209 MS.gene032937:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATTAAAATATCAAGA+AGG + chr5.4:28249127-28249146 MS.gene032937:intron 10.0%
!! AGAAATTAGAAATTGTTAAT+GGG + chr5.4:28248713-28248732 MS.gene032937:intron 15.0%
!! AAAAACTTTATCTTAATCAG+TGG + chr5.4:28249035-28249054 MS.gene032937:intron 20.0%
!! ATATACAACAGAATATCATA+TGG - chr5.4:28249154-28249173 None:intergenic 20.0%
!! CAGAAATTAGAAATTGTTAA+TGG + chr5.4:28248712-28248731 MS.gene032937:intron 20.0%
!!! ACTTTGTTCATCAATTTTAT+GGG - chr5.4:28249550-28249569 None:intergenic 20.0%
! AAATTGCAAAGAGTTCTATA+TGG + chr5.4:28250183-28250202 MS.gene032937:CDS 25.0%
! AACTCTTTGCAATTTCATTA+GGG - chr5.4:28250178-28250197 None:intergenic 25.0%
! ATTGACATTATAAAGCTATG+AGG - chr5.4:28249355-28249374 None:intergenic 25.0%
! GAATGTAAGTATATGTATCT+GGG - chr5.4:28249262-28249281 None:intergenic 25.0%
! GTCAAAATTCAACTACATTA+GGG + chr5.4:28250039-28250058 MS.gene032937:CDS 25.0%
! GTTATTCATTGTGATTAAAC+CGG - chr5.4:28248961-28248980 None:intergenic 25.0%
! TCATTGTTAATGATCTTACA+TGG + chr5.4:28249006-28249025 MS.gene032937:intron 25.0%
! TGAATGTAAGTATATGTATC+TGG - chr5.4:28249263-28249282 None:intergenic 25.0%
!! AAATGATGGAAAGAGATATA+AGG + chr5.4:28248580-28248599 MS.gene032937:CDS 25.0%
!! AATTCAACCATAAAGCTTTT+TGG + chr5.4:28248404-28248423 MS.gene032937:CDS 25.0%
!! ATATCTCTTTCCATCATTTT+CGG - chr5.4:28248579-28248598 None:intergenic 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5.4:28248405-28248424 MS.gene032937:CDS 25.0%
!! CACTTTGTTCATCAATTTTA+TGG - chr5.4:28249551-28249570 None:intergenic 25.0%
!!! TTAGTTTTCATGATGATAGA+TGG + chr5.4:28248846-28248865 MS.gene032937:intron 25.0%
AATCAGTATAATTGTGTTGC+AGG + chr5.4:28249380-28249399 MS.gene032937:intron 30.0%
AGTTCTCAAATTAATATCGG+TGG + chr5.4:28249294-28249313 MS.gene032937:intron 30.0%
ATAGATTGTACATTCCATGA+TGG - chr5.4:28250002-28250021 None:intergenic 30.0%
CAGTTTAGAAGTTTCTGTTT+CGG - chr5.4:28249523-28249542 None:intergenic 30.0%
CATATGTATATCCGTAAAAC+CGG + chr5.4:28248939-28248958 MS.gene032937:intron 30.0%
CATGAAAACTAACCAAAAAG+TGG - chr5.4:28248838-28248857 None:intergenic 30.0%
CGCAGTTCTCAAATTAATAT+CGG + chr5.4:28249291-28249310 MS.gene032937:intron 30.0%
GAACTCTTTGCAATTTCATT+AGG - chr5.4:28250179-28250198 None:intergenic 30.0%
GGTCAAAATTCAACTACATT+AGG + chr5.4:28250038-28250057 MS.gene032937:CDS 30.0%
GTTCTCAAATTAATATCGGT+GGG + chr5.4:28249295-28249314 MS.gene032937:intron 30.0%
TAGGGAAACATTCAAGAAAT+GGG + chr5.4:28250057-28250076 MS.gene032937:CDS 30.0%
TATCCCAATATAGCATACTA+TGG + chr5.4:28249963-28249982 MS.gene032937:CDS 30.0%
TTAGGGAAACATTCAAGAAA+TGG + chr5.4:28250056-28250075 MS.gene032937:CDS 30.0%
! ACGCATTTAATCTAAAGTGT+TGG - chr5.4:28249221-28249240 None:intergenic 30.0%
! ATTTTGAATCCTCTGTAGAT+TGG - chr5.4:28249455-28249474 None:intergenic 30.0%
! TAACTCTAAGGAAGAATCAT+TGG - chr5.4:28248468-28248487 None:intergenic 30.0%
! TGAATGAACTTTTATCCAGA+AGG - chr5.4:28249069-28249088 None:intergenic 30.0%
!! TCATGATTTGTTGACGATTT+CGG - chr5.4:28248914-28248933 None:intergenic 30.0%
!! TTGTGATTAAACCGGTTTTA+CGG - chr5.4:28248953-28248972 None:intergenic 30.0%
AATTCTCCAACCGAAAATGA+TGG + chr5.4:28248566-28248585 MS.gene032937:CDS 35.0%
ACAATTATGTCCGGTTTCAT+TGG - chr5.4:28249594-28249613 None:intergenic 35.0%
AGAAATATGATTGTAGGTGC+AGG + chr5.4:28249735-28249754 MS.gene032937:CDS 35.0%
AGAACTCATATCAGTTCAAG+AGG + chr5.4:28249416-28249435 MS.gene032937:CDS 35.0%
AGAAGCAAACTCTAACTCTA+AGG - chr5.4:28248480-28248499 None:intergenic 35.0%
AGAGAAATCTCCAATGAAAC+CGG + chr5.4:28249581-28249600 MS.gene032937:CDS 35.0%
AGCTCAGAGAAAGAAAAACT+TGG + chr5.4:28250098-28250117 MS.gene032937:CDS 35.0%
AGTACTACTATCTTGACCTT+TGG + chr5.4:28249798-28249817 MS.gene032937:CDS 35.0%
AGTAGAAGAATCTGATCCAA+AGG - chr5.4:28249817-28249836 None:intergenic 35.0%
AGTAGTACTATGCAATCCAT+TGG - chr5.4:28249787-28249806 None:intergenic 35.0%
AGTTCTATATGGTCAACACT+AGG + chr5.4:28250194-28250213 MS.gene032937:CDS 35.0%
ATATTGGGATAGAATGACAC+AGG - chr5.4:28249954-28249973 None:intergenic 35.0%
CTTGACAAGAAAGTGAATGT+TGG + chr5.4:28249852-28249871 MS.gene032937:CDS 35.0%
GCAACGAGAAATATGATTGT+AGG + chr5.4:28249729-28249748 MS.gene032937:CDS 35.0%
TCAACACTAGGAATCAAGAA+CGG + chr5.4:28250206-28250225 MS.gene032937:CDS 35.0%
TGAATAAGGACTATGATCAG+AGG - chr5.4:28248522-28248541 None:intergenic 35.0%
TGTTCTCCCAAAAAGCTTTA+TGG - chr5.4:28248414-28248433 None:intergenic 35.0%
! AAGTTTTTCTTTCTCTGAGC+TGG - chr5.4:28250099-28250118 None:intergenic 35.0%
!! ATGAGGTATTGACATAGACA+CGG - chr5.4:28249338-28249357 None:intergenic 35.0%
!! CTAATCCTGTAGCTTTATCA+AGG + chr5.4:28250306-28250325 MS.gene032937:CDS 35.0%
!! TCATCGATTCTTAGTGTCTT+AGG - chr5.4:28250155-28250174 None:intergenic 35.0%
!!! TTGCTGTTTAGACCACTTTT+TGG + chr5.4:28248823-28248842 MS.gene032937:intron 35.0%
AACAAACTCAATGCAGCCAA+TGG + chr5.4:28249768-28249787 MS.gene032937:CDS 40.0%
AACACTAGGAATCAAGAACG+GGG + chr5.4:28250208-28250227 MS.gene032937:CDS 40.0%
ACACAGGATAACACATTGCA+GGG - chr5.4:28249938-28249957 None:intergenic 40.0%
ACCGCCATAGTATGCTATAT+TGG - chr5.4:28249970-28249989 None:intergenic 40.0%
AGAGACCTTGATAAAGCTAC+AGG - chr5.4:28250314-28250333 None:intergenic 40.0%
ATCCTCTGTAGATTGGAATG+AGG - chr5.4:28249448-28249467 None:intergenic 40.0%
CAACACTAGGAATCAAGAAC+GGG + chr5.4:28250207-28250226 MS.gene032937:CDS 40.0%
CATATCAGTTCAAGAGGATG+AGG + chr5.4:28249422-28249441 MS.gene032937:CDS 40.0%
CCCAATATAGCATACTATGG+CGG + chr5.4:28249966-28249985 MS.gene032937:CDS 40.0%
CCGCCATAGTATGCTATATT+GGG - chr5.4:28249969-28249988 None:intergenic 40.0%
GAGGAATCTTCATGAGGAAA+AGG - chr5.4:28248503-28248522 None:intergenic 40.0%
GCCAATAAATAGAACCACCA+TGG - chr5.4:28248625-28248644 None:intergenic 40.0%
GGGGAACATGAAGATGAATA+AGG - chr5.4:28248536-28248555 None:intergenic 40.0%
TAAATAGAACCACCATGGCA+TGG - chr5.4:28248620-28248639 None:intergenic 40.0%
TATATGTATCTGGGTCGTGT+CGG - chr5.4:28249253-28249272 None:intergenic 40.0%
TCTATCTCGACTCAATCTTG+TGG + chr5.4:28250017-28250036 MS.gene032937:CDS 40.0%
TGATCAGAGGAATCTTCATG+AGG - chr5.4:28248509-28248528 None:intergenic 40.0%
TTGGAGAATTCACTTCAGGT+GGG - chr5.4:28248556-28248575 None:intergenic 40.0%
TTGTGTTGCAGGAAACATCA+TGG + chr5.4:28249391-28249410 MS.gene032937:intron 40.0%
! ATGCTTTTGCATCTTGGACA+GGG - chr5.4:28249618-28249637 None:intergenic 40.0%
! CTCTTTCCATCATTTTCGGT+TGG - chr5.4:28248575-28248594 None:intergenic 40.0%
! CTTTTATCCAGAAGGACTTC+AGG - chr5.4:28249061-28249080 None:intergenic 40.0%
! GCCATGGTGGTTCTATTTAT+TGG + chr5.4:28248621-28248640 MS.gene032937:intron 40.0%
! GTTTACCTCCACAGTTTTCT+TGG + chr5.4:28249910-28249929 MS.gene032937:CDS 40.0%
!! ATTAGCTTGCAAAACTGACG+AGG - chr5.4:28250291-28250310 None:intergenic 40.0%
!! GTGTTGGTGTTACATAGACA+TGG - chr5.4:28249205-28249224 None:intergenic 40.0%
!! TGAGGTATTGACATAGACAC+GGG - chr5.4:28249337-28249356 None:intergenic 40.0%
ACACTAGGAATCAAGAACGG+GGG + chr5.4:28250209-28250228 MS.gene032937:CDS 45.0%
ACTTCTGCAAAAACTGCCAG+AGG + chr5.4:28249688-28249707 MS.gene032937:CDS 45.0%
AGAAGTTTCTGTTTCGGACG+AGG - chr5.4:28249517-28249536 None:intergenic 45.0%
AGCATACTATGGCGGTTGTT+TGG + chr5.4:28249974-28249993 MS.gene032937:CDS 45.0%
CATATTTCTCGTTGCTCCTC+CGG - chr5.4:28249724-28249743 None:intergenic 45.0%
CTGTCCAAGATGCAAAAGCA+TGG + chr5.4:28249617-28249636 MS.gene032937:CDS 45.0%
GAAGTTTCTGTTTCGGACGA+GGG - chr5.4:28249516-28249535 None:intergenic 45.0%
GACACAGGATAACACATTGC+AGG - chr5.4:28249939-28249958 None:intergenic 45.0%
GGACAGGGAACAATTATGTC+CGG - chr5.4:28249603-28249622 None:intergenic 45.0%
GTTGGAGAATTCACTTCAGG+TGG - chr5.4:28248557-28248576 None:intergenic 45.0%
TCATGGGATTCGCATCCAAA+GGG + chr5.4:28250240-28250259 MS.gene032937:CDS 45.0%
TCGGTTGGAGAATTCACTTC+AGG - chr5.4:28248560-28248579 None:intergenic 45.0%
TGGAGAATTCACTTCAGGTG+GGG - chr5.4:28248555-28248574 None:intergenic 45.0%
TTCATGGGATTCGCATCCAA+AGG + chr5.4:28250239-28250258 MS.gene032937:CDS 45.0%
! CATGCTTTTGCATCTTGGAC+AGG - chr5.4:28249619-28249638 None:intergenic 45.0%
! GCAAAAACTGCCAGAGGTAT+TGG + chr5.4:28249694-28249713 MS.gene032937:CDS 45.0%
! GTGTCCATGCTTTTGCATCT+TGG - chr5.4:28249624-28249643 None:intergenic 45.0%
!! GGCAGTTTTTGCAGAAGTGT+CGG - chr5.4:28249686-28249705 None:intergenic 45.0%
!!! CAGTTTTTGCAGAAGTGTCG+GGG - chr5.4:28249684-28249703 None:intergenic 45.0%
!!! GAGTTTTCGGGTTCTCAACA+AGG - chr5.4:28249494-28249513 None:intergenic 45.0%
!!! GCAGTTTTTGCAGAAGTGTC+GGG - chr5.4:28249685-28249704 None:intergenic 45.0%
!! ATTTATTTCAAAATAAAATT+TGG - chr5.4:28248744-28248763 None:intergenic 5.0%
ACGATTCTTGACTCCCCCTT+TGG - chr5.4:28250258-28250277 None:intergenic 50.0%
ATGGGATTCGCATCCAAAGG+GGG + chr5.4:28250242-28250261 MS.gene032937:CDS 50.0%
CATGGGATTCGCATCCAAAG+GGG + chr5.4:28250241-28250260 MS.gene032937:CDS 50.0%
GCAGGGTTCCAAGAAAACTG+TGG - chr5.4:28249921-28249940 None:intergenic 50.0%
GCGGTTGTTTGGTACCATCA+TGG + chr5.4:28249985-28250004 MS.gene032937:CDS 50.0%
GGCCTCATTCCAATCTACAG+AGG + chr5.4:28249443-28249462 MS.gene032937:CDS 50.0%
GGGTTCCAAGAAAACTGTGG+AGG - chr5.4:28249918-28249937 None:intergenic 50.0%
GGGTTCTCAACAAGGCTTGA+AGG - chr5.4:28249486-28249505 None:intergenic 50.0%
GGTCTCTTGTGTTCCATGAG+AGG + chr5.4:28250327-28250346 MS.gene032937:CDS 50.0%
TGAGTTCTTGTGCCATGCCA+TGG + chr5.4:28248605-28248624 MS.gene032937:intron 50.0%
! AACGGGGGAGAGCTTTTCAT+GGG + chr5.4:28250224-28250243 MS.gene032937:CDS 50.0%
! GTTTCGGACGAGGGAGTTTT+CGG - chr5.4:28249507-28249526 None:intergenic 50.0%
! TTTCGGACGAGGGAGTTTTC+GGG - chr5.4:28249506-28249525 None:intergenic 50.0%
!! TGCCAGAGGTATTGGACTTC+CGG + chr5.4:28249702-28249721 MS.gene032937:CDS 50.0%
CTCCGGAAGTCCAATACCTC+TGG - chr5.4:28249707-28249726 None:intergenic 55.0%
GTTCTTGTGCCATGCCATGG+TGG + chr5.4:28248608-28248627 MS.gene032937:intron 55.0%
TCAGTGGCCTGAAGTCCTTC+TGG + chr5.4:28249051-28249070 MS.gene032937:intron 55.0%
! GAACGGGGGAGAGCTTTTCA+TGG + chr5.4:28250223-28250242 MS.gene032937:CDS 55.0%
!! CAGAGGTATTGGACTTCCGG+AGG + chr5.4:28249705-28249724 MS.gene032937:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 28248395 28250355 28248395 ID=MS.gene032937
chr5.4 mRNA 28248395 28250355 28248395 ID=MS.gene032937.t1;Parent=MS.gene032937
chr5.4 exon 28248395 28248601 28248395 ID=MS.gene032937.t1.exon1;Parent=MS.gene032937.t1
chr5.4 CDS 28248395 28248601 28248395 ID=cds.MS.gene032937.t1;Parent=MS.gene032937.t1
chr5.4 exon 28249402 28250355 28249402 ID=MS.gene032937.t1.exon2;Parent=MS.gene032937.t1
chr5.4 CDS 28249402 28250355 28249402 ID=cds.MS.gene032937.t1;Parent=MS.gene032937.t1
Gene Sequence

>MS.gene032937

ATGACTAATAATTCAACCATAAAGCTTTTTGGGAGAACAATTTTTCAAACATGCAACACAGATGTCTCTACCAATGATTCTTCCTTAGAGTTAGAGTTTGCTTCTCCTTTTCCTCATGAAGATTCCTCTGATCATAGTCCTTATTCATCTTCATGTTCCCCACCTGAAGTGAATTCTCCAACCGAAAATGATGGAAAGAGATATAAGGTATGAGTTCTTGTGCCATGCCATGGTGGTTCTATTTATTGGCTAAAATTTATTATTTAAAATATGTTGCTAACTAGTGTGTTTTGAAATTATAACAGTGCAACTTTTTTCAGAAATTAGAAATTGTTAATGGGTTGATCCAAATTTTATTTTGAAATAAATTGCTTCTAGTTAAGACTTAAGAGTATATAATTATAATTAAGTCTCACAGCAATTTTTCGTTGCTGTTTAGACCACTTTTTGGTTAGTTTTCATGATGATAGATGGTTGATGAGATTGTTATCTTTTCTCATTAGTAGATTTATTTAACCGAAATCGTCAACAAATCATGATCAATCATATGTATATCCGTAAAACCGGTTTAATCACAATGAATAACACGACTCAATCATGATTAAAGTCAATCATTGTTAATGATCTTACATGGTTTTATAAAAACTTTATCTTAATCAGTGGCCTGAAGTCCTTCTGGATAAAAGTTCATTCAAGAATTTCGTGTTCTAAAACATGAGATTGCAAAACTGAAATAAATTAAAATATCAAGAAGGACCATATGATATTCTGTTGTATATTTATGTCAAAACGAATAAATCATTTAGTCCATGTCTATGTAACACCAACACTTTAGATTAAATGCGTGTTTGATGTCCGACACGACCCAGATACATATACTTACATTCAATATTTCACGCAGTTCTCAAATTAATATCGGTGGGAGTGTCGTATTCGATTCCCGTGTCTATGTCAATACCTCATAGCTTTATAATGTCAATTTATAAATCAGTATAATTGTGTTGCAGGAAACATCATGGAAAGAACTCATATCAGTTCAAGAGGATGAGGCCTCATTCCAATCTACAGAGGATTCAAAATCTCACACACCTTCAAGCCTTGTTGAGAACCCGAAAACTCCCTCGTCCGAAACAGAAACTTCTAAACTGAATTCCCATAAAATTGATGAACAAAGTGATATGTCACAAGAGAAATCTCCAATGAAACCGGACATAATTGTTCCCTGTCCAAGATGCAAAAGCATGGACACAAAATTTTGTTACTATAACAACTACAATGTCAAACAGCCCCGACACTTCTGCAAAAACTGCCAGAGGTATTGGACTTCCGGAGGAGCAACGAGAAATATGATTGTAGGTGCAGGTCGTCGCAAGAACAAACTCAATGCAGCCAATGGATTGCATAGTACTACTATCTTGACCTTTGGATCAGATTCTTCTACTATGTCTTCAACTTCACTTGACAAGAAAGTGAATGTTGGATCACATGAAGAAACATTTGATAAGAGTTATCAAAGTTTACCTCCACAGTTTTCTTGGAACCCTGCAATGTGTTATCCTGTGTCATTCTATCCCAATATAGCATACTATGGCGGTTGTTTGGTACCATCATGGAATGTACAATCTATCTCGACTCAATCTTGTGGTCAAAATTCAACTACATTAGGGAAACATTCAAGAAATGGGAACATTCTCGTTTGTTCCAGCTCAGAGAAAGAAAAACTTGGATCAGAAAGTAATAATAATAGTGTGTTCATACCTAAGACACTAAGAATCGATGACCCTAATGAAATTGCAAAGAGTTCTATATGGTCAACACTAGGAATCAAGAACGGGGGAGAGCTTTTCATGGGATTCGCATCCAAAGGGGGAGTCAAGAATCGTGTTGAAACATCCTCGTCAGTTTTGCAAGCTAATCCTGTAGCTTTATCAAGGTCTCTTGTGTTCCATGAGAGGATTTGA
Protein sequence

>MS.gene032937.t1

MTNNSTIKLFGRTIFQTCNTDVSTNDSSLELEFASPFPHEDSSDHSPYSSSCSPPEVNSPTENDGKRYKETSWKELISVQEDEASFQSTEDSKSHTPSSLVENPKTPSSETETSKLNSHKIDEQSDMSQEKSPMKPDIIVPCPRCKSMDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGATRNMIVGAGRRKNKLNAANGLHSTTILTFGSDSSTMSSTSLDKKVNVGSHEETFDKSYQSLPPQFSWNPAMCYPVSFYPNIAYYGGCLVPSWNVQSISTQSCGQNSTTLGKHSRNGNILVCSSSEKEKLGSESNNNSVFIPKTLRIDDPNEIAKSSIWSTLGIKNGGELFMGFASKGGVKNRVETSSSVLQANPVALSRSLVFHERI