AlfalfaGEDB | MS.gene032937

M. sativa cultivar XinJiangDaYe / MS.gene032937

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02701.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032937.t1	XP_003613833.1	87.3	386	44	1	1	386	1	381	1.80E-179	638.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032937.t1	Q8W1E3	44.7	284	129	10	115	386	31	298	7.5e-49	196.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032937.t1	G7KGM5	87.3	386	44	1	1	386	1	381	1.3e-179	638.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene032937.t1	TF	C2C2-Dof

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032937.t1	MTR_5g041530	88.601	386	39	1	1	386	1	381	0.0	650
MS.gene032937.t1	MTR_5g041380	63.131	396	111	9	1	386	1	371	7.59e-154	438
MS.gene032937.t1	MTR_5g041400	61.675	394	112	9	1	386	1	363	2.15e-148	424
MS.gene032937.t1	MTR_5g041420	72.787	305	71	4	1	297	1	301	1.42e-141	405
MS.gene032937.t1	MTR_4g082060	42.029	483	147	19	7	384	9	463	5.98e-91	281
MS.gene032937.t1	MTR_7g010950	36.970	495	186	15	2	384	4	484	2.54e-70	229
MS.gene032937.t1	MTR_6g012450	37.292	421	147	13	80	384	74	493	1.20e-64	214
MS.gene032937.t1	MTR_3g435480	36.496	411	153	14	69	384	66	463	3.08e-59	199
MS.gene032937.t1	MTR_8g044220	32.133	361	142	10	115	386	93	439	1.73e-47	167
MS.gene032937.t1	MTR_6g027460	55.140	107	45	1	98	201	51	157	8.97e-33	126

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene032937.t1	AT5G62430	44.828	290	132	10	109	386	25	298	2.50e-64	207
MS.gene032937.t1	AT5G39660	37.852	391	158	14	70	384	74	455	6.31e-58	196
MS.gene032937.t1	AT5G39660	37.852	391	158	14	70	384	74	455	6.31e-58	196
MS.gene032937.t1	AT5G39660	37.852	391	158	14	70	384	74	455	6.31e-58	196
MS.gene032937.t1	AT1G69570	37.092	337	145	17	98	386	80	397	9.53e-47	164
MS.gene032937.t1	AT1G26790	36.042	283	134	10	135	386	106	372	4.85e-38	140
MS.gene032937.t1	AT1G29160	73.913	69	15	1	125	193	50	115	1.63e-32	120
MS.gene032937.t1	AT2G34140	57.143	91	27	2	125	206	46	133	1.02e-30	115
MS.gene032937.t1	AT3G47500	63.415	82	30	0	122	203	92	173	4.95e-30	120
MS.gene032937.t1	AT3G47500	42.945	163	70	6	240	386	293	448	1.08e-23	102

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Find 71 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGATGGAAAGAGATATA+AGG	0.429316	5.4:+28248580	MS.gene032937:CDS
AAATTGCAAAGAGTTCTATA+TGG	0.517606	5.4:+28250183	MS.gene032937:CDS
AACAAACTCAATGCAGCCAA+TGG	0.545923	5.4:+28249768	MS.gene032937:CDS
AACACTAGGAATCAAGAACG+GGG	0.710662	5.4:+28250208	MS.gene032937:CDS
AACTCTTTGCAATTTCATTA+GGG	0.357550	5.4:-28250175	None:intergenic
AATTCTCCAACCGAAAATGA+TGG	0.512006	5.4:+28248566	MS.gene032937:CDS
ACAATTATGTCCGGTTTCAT+TGG	0.357374	5.4:-28249591	None:intergenic
ACACAGGATAACACATTGCA+GGG	0.622856	5.4:-28249935	None:intergenic
ACACTAGGAATCAAGAACGG+GGG	0.749858	5.4:+28250209	MS.gene032937:CDS
ACCGCCATAGTATGCTATAT+TGG	0.313531	5.4:-28249967	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCAGTATAATTGTGTTGC+AGG	+	chr5.4:28249380-28249399	MS.gene032937:intron	30.0%
AGTTCTCAAATTAATATCGG+TGG	+	chr5.4:28249294-28249313	MS.gene032937:intron	30.0%
ATAGATTGTACATTCCATGA+TGG	-	chr5.4:28250002-28250021	None:intergenic	30.0%
CAGTTTAGAAGTTTCTGTTT+CGG	-	chr5.4:28249523-28249542	None:intergenic	30.0%
CATATGTATATCCGTAAAAC+CGG	+	chr5.4:28248939-28248958	MS.gene032937:intron	30.0%
CATGAAAACTAACCAAAAAG+TGG	-	chr5.4:28248838-28248857	None:intergenic	30.0%
CGCAGTTCTCAAATTAATAT+CGG	+	chr5.4:28249291-28249310	MS.gene032937:intron	30.0%
GAACTCTTTGCAATTTCATT+AGG	-	chr5.4:28250179-28250198	None:intergenic	30.0%
GGTCAAAATTCAACTACATT+AGG	+	chr5.4:28250038-28250057	MS.gene032937:CDS	30.0%
GTTCTCAAATTAATATCGGT+GGG	+	chr5.4:28249295-28249314	MS.gene032937:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	28248395	28250355	28248395	ID=MS.gene032937
chr5.4	mRNA	28248395	28250355	28248395	ID=MS.gene032937.t1;Parent=MS.gene032937
chr5.4	exon	28248395	28248601	28248395	ID=MS.gene032937.t1.exon1;Parent=MS.gene032937.t1
chr5.4	CDS	28248395	28248601	28248395	ID=cds.MS.gene032937.t1;Parent=MS.gene032937.t1
chr5.4	exon	28249402	28250355	28249402	ID=MS.gene032937.t1.exon2;Parent=MS.gene032937.t1
chr5.4	CDS	28249402	28250355	28249402	ID=cds.MS.gene032937.t1;Parent=MS.gene032937.t1

Gene Sequence

>MS.gene032937

ATGACTAATAATTCAACCATAAAGCTTTTTGGGAGAACAATTTTTCAAACATGCAACACAGATGTCTCTACCAATGATTCTTCCTTAGAGTTAGAGTTTGCTTCTCCTTTTCCTCATGAAGATTCCTCTGATCATAGTCCTTATTCATCTTCATGTTCCCCACCTGAAGTGAATTCTCCAACCGAAAATGATGGAAAGAGATATAAGGTATGAGTTCTTGTGCCATGCCATGGTGGTTCTATTTATTGGCTAAAATTTATTATTTAAAATATGTTGCTAACTAGTGTGTTTTGAAATTATAACAGTGCAACTTTTTTCAGAAATTAGAAATTGTTAATGGGTTGATCCAAATTTTATTTTGAAATAAATTGCTTCTAGTTAAGACTTAAGAGTATATAATTATAATTAAGTCTCACAGCAATTTTTCGTTGCTGTTTAGACCACTTTTTGGTTAGTTTTCATGATGATAGATGGTTGATGAGATTGTTATCTTTTCTCATTAGTAGATTTATTTAACCGAAATCGTCAACAAATCATGATCAATCATATGTATATCCGTAAAACCGGTTTAATCACAATGAATAACACGACTCAATCATGATTAAAGTCAATCATTGTTAATGATCTTACATGGTTTTATAAAAACTTTATCTTAATCAGTGGCCTGAAGTCCTTCTGGATAAAAGTTCATTCAAGAATTTCGTGTTCTAAAACATGAGATTGCAAAACTGAAATAAATTAAAATATCAAGAAGGACCATATGATATTCTGTTGTATATTTATGTCAAAACGAATAAATCATTTAGTCCATGTCTATGTAACACCAACACTTTAGATTAAATGCGTGTTTGATGTCCGACACGACCCAGATACATATACTTACATTCAATATTTCACGCAGTTCTCAAATTAATATCGGTGGGAGTGTCGTATTCGATTCCCGTGTCTATGTCAATACCTCATAGCTTTATAATGTCAATTTATAAATCAGTATAATTGTGTTGCAGGAAACATCATGGAAAGAACTCATATCAGTTCAAGAGGATGAGGCCTCATTCCAATCTACAGAGGATTCAAAATCTCACACACCTTCAAGCCTTGTTGAGAACCCGAAAACTCCCTCGTCCGAAACAGAAACTTCTAAACTGAATTCCCATAAAATTGATGAACAAAGTGATATGTCACAAGAGAAATCTCCAATGAAACCGGACATAATTGTTCCCTGTCCAAGATGCAAAAGCATGGACACAAAATTTTGTTACTATAACAACTACAATGTCAAACAGCCCCGACACTTCTGCAAAAACTGCCAGAGGTATTGGACTTCCGGAGGAGCAACGAGAAATATGATTGTAGGTGCAGGTCGTCGCAAGAACAAACTCAATGCAGCCAATGGATTGCATAGTACTACTATCTTGACCTTTGGATCAGATTCTTCTACTATGTCTTCAACTTCACTTGACAAGAAAGTGAATGTTGGATCACATGAAGAAACATTTGATAAGAGTTATCAAAGTTTACCTCCACAGTTTTCTTGGAACCCTGCAATGTGTTATCCTGTGTCATTCTATCCCAATATAGCATACTATGGCGGTTGTTTGGTACCATCATGGAATGTACAATCTATCTCGACTCAATCTTGTGGTCAAAATTCAACTACATTAGGGAAACATTCAAGAAATGGGAACATTCTCGTTTGTTCCAGCTCAGAGAAAGAAAAACTTGGATCAGAAAGTAATAATAATAGTGTGTTCATACCTAAGACACTAAGAATCGATGACCCTAATGAAATTGCAAAGAGTTCTATATGGTCAACACTAGGAATCAAGAACGGGGGAGAGCTTTTCATGGGATTCGCATCCAAAGGGGGAGTCAAGAATCGTGTTGAAACATCCTCGTCAGTTTTGCAAGCTAATCCTGTAGCTTTATCAAGGTCTCTTGTGTTCCATGAGAGGATTTGA

Protein sequence

>MS.gene032937.t1

MTNNSTIKLFGRTIFQTCNTDVSTNDSSLELEFASPFPHEDSSDHSPYSSSCSPPEVNSPTENDGKRYKETSWKELISVQEDEASFQSTEDSKSHTPSSLVENPKTPSSETETSKLNSHKIDEQSDMSQEKSPMKPDIIVPCPRCKSMDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGATRNMIVGAGRRKNKLNAANGLHSTTILTFGSDSSTMSSTSLDKKVNVGSHEETFDKSYQSLPPQFSWNPAMCYPVSFYPNIAYYGGCLVPSWNVQSISTQSCGQNSTTLGKHSRNGNILVCSSSEKEKLGSESNNNSVFIPKTLRIDDPNEIAKSSIWSTLGIKNGGELFMGFASKGGVKNRVETSSSVLQANPVALSRSLVFHERI