AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033046

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033046.t1 MTR_4g109770 96.383 470 17 0 1 470 1 470 0.0 939
MS.gene033046.t1 MTR_2g461920 62.272 493 147 11 12 470 52 539 1.26e-172 496
MS.gene033046.t1 MTR_5g046670 49.315 219 88 8 56 264 174 379 1.35e-50 179
MS.gene033046.t1 MTR_3g099080 54.348 138 42 4 75 198 43 173 4.03e-38 143
MS.gene033046.t1 MTR_7g028740 35.331 317 179 8 75 382 26 325 7.89e-38 142
MS.gene033046.t1 MTR_8g096780 38.194 288 132 11 75 339 45 309 1.08e-36 139
MS.gene033046.t1 MTR_7g092930 69.136 81 25 0 117 197 62 142 7.00e-34 130
MS.gene033046.t1 MTR_7g444860 75.676 74 18 0 123 196 149 222 7.18e-34 130
MS.gene033046.t1 MTR_3g085180 50.769 130 57 2 72 198 115 240 8.48e-34 132
MS.gene033046.t1 MTR_1g053715 38.197 233 106 6 123 351 74 272 1.87e-33 129
MS.gene033046.t1 MTR_8g005960 73.684 76 20 0 123 198 187 262 4.55e-33 128
MS.gene033046.t1 MTR_4g088555 72.973 74 20 0 123 196 63 136 7.87e-33 122
MS.gene033046.t1 MTR_2g014200 70.886 79 23 0 118 196 59 137 3.24e-32 120
MS.gene033046.t1 MTR_8g463140 70.270 74 22 0 123 196 65 138 2.83e-31 119
MS.gene033046.t1 MTR_2g078770 70.270 74 22 0 123 196 58 131 1.23e-30 117
MS.gene033046.t1 MTR_7g110320 69.737 76 23 0 121 196 143 218 1.46e-30 125
MS.gene033046.t1 MTR_2g046550 45.946 148 69 3 58 196 67 212 2.41e-30 125
MS.gene033046.t1 MTR_7g110320 69.737 76 23 0 121 196 143 218 2.63e-30 125
MS.gene033046.t1 MTR_1g035010 62.366 93 31 1 110 198 87 179 1.53e-29 123
MS.gene033046.t1 MTR_1g086250 69.737 76 23 0 121 196 145 220 1.86e-29 122
MS.gene033046.t1 MTR_8g080680 36.607 224 103 8 122 339 131 321 3.11e-28 116
MS.gene033046.t1 MTR_8g080690 63.158 76 28 0 123 198 151 226 5.49e-28 115
MS.gene033046.t1 MTR_8g080670 61.644 73 28 0 123 195 162 234 4.33e-25 107
MS.gene033046.t1 MTR_2g020620 51.562 64 31 0 133 196 100 163 2.28e-16 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033046.t1 AT1G69170 64.286 126 32 3 75 197 83 198 8.83e-45 162
MS.gene033046.t1 AT5G50670 46.067 178 84 4 123 288 101 278 2.50e-37 140
MS.gene033046.t1 AT5G50670 46.067 178 84 4 123 288 101 278 2.50e-37 140
MS.gene033046.t1 AT5G50570 46.067 178 84 4 123 288 101 278 2.50e-37 140
MS.gene033046.t1 AT5G50570 46.067 178 84 4 123 288 101 278 2.50e-37 140
MS.gene033046.t1 AT1G27370 68.966 87 27 0 123 209 176 262 2.30e-36 137
MS.gene033046.t1 AT1G27370 68.966 87 27 0 123 209 176 262 6.18e-36 137
MS.gene033046.t1 AT1G27370 68.966 87 27 0 123 209 176 262 6.18e-36 137
MS.gene033046.t1 AT1G27370 68.966 87 27 0 123 209 176 262 6.18e-36 137
MS.gene033046.t1 AT1G27370 68.966 87 27 0 123 209 176 262 6.18e-36 137
MS.gene033046.t1 AT1G27370 68.966 87 27 0 123 209 176 262 6.18e-36 137
MS.gene033046.t1 AT1G27370 68.966 87 27 0 123 209 176 262 6.18e-36 137
MS.gene033046.t1 AT1G27360 46.429 168 65 4 53 209 108 261 4.73e-35 135
MS.gene033046.t1 AT1G27360 46.429 168 65 4 53 209 108 261 4.73e-35 135
MS.gene033046.t1 AT1G27360 46.429 168 65 4 53 209 108 261 4.73e-35 135
MS.gene033046.t1 AT1G27360 46.429 168 65 4 53 209 108 261 4.73e-35 135
MS.gene033046.t1 AT5G43270 46.199 171 68 6 48 209 94 249 7.13e-34 132
MS.gene033046.t1 AT5G43270 46.199 171 68 6 48 209 94 249 7.13e-34 132
MS.gene033046.t1 AT5G43270 46.199 171 68 6 48 209 94 249 7.13e-34 132
MS.gene033046.t1 AT2G33810 67.089 79 26 0 118 196 49 127 6.11e-33 122
MS.gene033046.t1 AT3G57920 70.588 85 25 0 114 198 50 134 1.32e-32 127
MS.gene033046.t1 AT2G42200 75.000 76 19 0 123 198 74 149 1.79e-32 127
MS.gene033046.t1 AT1G02065 72.727 77 21 0 120 196 185 261 2.05e-32 126
MS.gene033046.t1 AT2G47070 73.684 76 20 0 121 196 104 179 2.77e-31 128
MS.gene033046.t1 AT3G60030 72.973 74 20 0 123 196 127 200 4.71e-31 127
MS.gene033046.t1 AT1G53160 51.667 120 48 3 123 232 54 173 4.97e-31 118
MS.gene033046.t1 AT1G53160 51.667 120 48 3 123 232 54 173 4.97e-31 118
MS.gene033046.t1 AT1G20980 62.000 100 35 1 110 206 104 203 8.42e-31 127
MS.gene033046.t1 AT1G76580 72.368 76 21 0 123 198 114 189 3.27e-30 124
MS.gene033046.t1 AT1G76580 72.368 76 21 0 123 198 114 189 3.43e-30 124
MS.gene033046.t1 AT1G53160 62.069 87 31 1 123 207 54 140 4.37e-30 115
MS.gene033046.t1 AT1G76580 72.368 76 21 0 123 198 114 189 6.60e-30 124
MS.gene033046.t1 AT3G15270 63.636 77 28 0 123 199 63 139 1.03e-28 112
MS.gene033046.t1 AT5G18830 54.023 87 39 1 112 197 127 213 2.07e-22 101
MS.gene033046.t1 AT5G18830 54.023 87 39 1 112 197 127 213 2.21e-22 101
MS.gene033046.t1 AT5G18830 54.023 87 39 1 112 197 127 213 2.32e-22 100
MS.gene033046.t1 AT2G47070 76.000 50 12 0 147 196 1 50 5.01e-18 87.4
MS.gene033046.t1 AT1G02065 64.706 51 18 0 120 170 185 235 2.48e-16 79.0

Find 96 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCATATTGTCAAGTTTA+TGG 0.148069 8.2:+16190318 MS.gene033046:CDS
AGGTCTTCCAAAGTGGTTTC+TGG 0.202370 8.2:+16191836 MS.gene033046:CDS
GGCGTTCACTCTACAAATTC+TGG 0.217076 8.2:+16191258 MS.gene033046:CDS
CTGCTGGTGGCTTGGTTCCC+TGG 0.251209 8.2:-16191795 None:intergenic
GGACTGGCTTGAAGTGTTAT+TGG 0.259748 8.2:-16192166 None:intergenic
CGAAATCACTTTAAGTAATT+CGG 0.269479 8.2:-16192545 None:intergenic
AGTTATATTTCTGAAGAAAA+AGG 0.280021 8.2:+16189973 MS.gene033046:CDS
CACAAACTTTATGCCTCTTA+TGG 0.303462 8.2:-16190374 None:intergenic
CAAAATAGGTGCTGCTCCTT+TGG 0.306496 8.2:-16190244 None:intergenic
GGGAGACTGTTTCAGCCATT+TGG 0.327396 8.2:+16191279 MS.gene033046:CDS
TATGGCCTGCAAGTCGTTTA+CGG 0.342741 8.2:-16191212 None:intergenic
TCAGATGATTTCAATTTCAT+TGG 0.348900 8.2:+16190039 MS.gene033046:CDS
CCAAGCATTCAAGGATTATC+AGG 0.351371 8.2:+16192102 MS.gene033046:CDS
GCTTCATGTTCTGCTTTCAT+AGG 0.354620 8.2:-16191862 None:intergenic
TCCATCTGTAGGATTTAATT+CGG 0.357577 8.2:-16192298 None:intergenic
TTATTGTCTGGTACCAATAT+AGG 0.364521 8.2:-16192333 None:intergenic
GTTCCAAGCAAGAAACTTGA+TGG 0.403736 8.2:+16190066 MS.gene033046:CDS
TGAGTGCACTCTCACTCTCT+TGG 0.404810 8.2:-16190292 None:intergenic
CATACCGCACCAAGCATTCA+AGG 0.405157 8.2:+16192093 MS.gene033046:CDS
CAAGGATCTTAGTTCTTGTA+AGG 0.406648 8.2:+16190347 MS.gene033046:CDS
TGATATCGTGAACTTTGAAA+TGG 0.410198 8.2:+16192356 MS.gene033046:CDS
GACTCACAAGAAGACAAAAT+AGG 0.410388 8.2:-16190258 None:intergenic
TCTTGCAGATATCAGATTCC+AGG 0.416127 8.2:+16191777 MS.gene033046:intron
AATTCCCTTTGATAACCCTT+TGG 0.423487 8.2:+16192194 MS.gene033046:CDS
CAGAACATGAAGCTGGCTTT+AGG 0.424799 8.2:+16191872 MS.gene033046:CDS
TGTTGCATGCAATGGAATCT+TGG 0.426426 8.2:+16189950 None:intergenic
GAGTGCACTCTCACTCTCTT+GGG 0.430597 8.2:-16190291 None:intergenic
TTACCATCAAGTTTCTTGCT+TGG 0.431934 8.2:-16190069 None:intergenic
AGTTGCCGTAAACGACTTGC+AGG 0.435658 8.2:+16191207 MS.gene033046:CDS
CCTGATAATCCTTGAATGCT+TGG 0.438418 8.2:-16192102 None:intergenic
AAGTCATCTCAGTCCTATAT+TGG 0.441537 8.2:+16192320 MS.gene033046:CDS
ATTCCATTCACAATGACTAT+AGG 0.443748 8.2:-16190414 None:intergenic
CAAAGGGAATTTCCAAGGAC+TGG 0.449402 8.2:-16192182 None:intergenic
TCTCTCATTGATTTGAAGCT+AGG 0.463051 8.2:+16190177 MS.gene033046:CDS
CACAATGACTATAGGAGTCT+TGG 0.465991 8.2:-16190406 None:intergenic
TAACACTTCAAGCCAGTCCT+TGG 0.468157 8.2:+16192170 MS.gene033046:CDS
TCTTCTGTGCCTGTCACCAA+TGG 0.476821 8.2:+16191901 MS.gene033046:CDS
GCATCATAGACAATCATTGC+AGG 0.480777 8.2:+16192491 MS.gene033046:CDS
GCGTTCACTCTACAAATTCT+GGG 0.481125 8.2:+16191259 MS.gene033046:CDS
TTCACGATATCATTATTGTC+TGG 0.484373 8.2:-16192345 None:intergenic
CATTCACAAAATCTGAATCA+TGG 0.487792 8.2:-16192392 None:intergenic
CTAGGAAGATTTGATGATCA+TGG 0.493709 8.2:+16190195 MS.gene033046:CDS
GCCATTGGCTACACCTCCTT+AGG 0.499701 8.2:+16192048 MS.gene033046:CDS
ATTGAAGTCTTCGCTTCCTA+AGG 0.501723 8.2:-16192064 None:intergenic
TATATATTATTACCACCAAA+TGG 0.502996 8.2:-16191294 None:intergenic
AAAAGATGCTGCTGGTGGCT+TGG 0.511710 8.2:-16191803 None:intergenic
TAGACATGGCTATGGCTGCT+TGG 0.512721 8.2:-16192222 None:intergenic
TGTGGTGGAATCCAATGGTT+GGG 0.513935 8.2:+16190152 MS.gene033046:CDS
ACTCCTATAGTCATTGTGAA+TGG 0.516933 8.2:+16190411 MS.gene033046:CDS
TTGCTGTCTGAGTTTGATGA+TGG 0.518570 8.2:+16191177 MS.gene033046:CDS
TCCGAATTAAATCCTACAGA+TGG 0.523648 8.2:+16192297 MS.gene033046:CDS
AATGAGAGAATCCCAACCAT+TGG 0.523865 8.2:-16190163 None:intergenic
AAACCACTTTGGAAGACCTC+TGG 0.529651 8.2:-16191832 None:intergenic
AGCCATTATGCTCATGCCAT+TGG 0.530681 8.2:+16192033 MS.gene033046:CDS
AGCCAATGGCATGAGCATAA+TGG 0.532501 8.2:-16192035 None:intergenic
TAGGTCTCCAGAAACCACTT+TGG 0.532795 8.2:-16191843 None:intergenic
CTTGTAAGGACTACCATAAG+AGG 0.534199 8.2:+16190361 MS.gene033046:CDS
TTTCAAGCTTTCAAATTCTG+TGG 0.537070 8.2:+16190134 MS.gene033046:CDS
TTGCATCATCTTCACAAGAA+AGG 0.541164 8.2:-16192425 None:intergenic
TTATGCTGTAGACATGGCTA+TGG 0.541912 8.2:-16192230 None:intergenic
AATGTTGCCACATCCACTAC+AGG 0.543311 8.2:+16191988 MS.gene033046:CDS
ATAACCAAAGGGTTATCAAA+GGG 0.544827 8.2:-16192198 None:intergenic
CAAGCTTTCAAATTCTGTGG+TGG 0.545205 8.2:+16190137 MS.gene033046:CDS
GTTATCAAAGGGAATTTCCA+AGG 0.547080 8.2:-16192187 None:intergenic
AGACTGTTTCAGCCATTTGG+TGG 0.551343 8.2:+16191282 MS.gene033046:CDS
GGATAGAGAAAAGATGCTGC+TGG 0.557359 8.2:-16191811 None:intergenic
AATAACCAAAGGGTTATCAA+AGG 0.561243 8.2:-16192199 None:intergenic
TGGCTGCTTGGAATAACCAA+AGG 0.562021 8.2:-16192210 None:intergenic
CTGTGGTGGAATCCAATGGT+TGG 0.564108 8.2:+16190151 MS.gene033046:CDS
ATGAAAGCAGAACATGAAGC+TGG 0.566946 8.2:+16191865 MS.gene033046:CDS
GGCTGCTTGGAATAACCAAA+GGG 0.567623 8.2:-16192209 None:intergenic
GAAGTCTTCGCTTCCTAAGG+AGG 0.569996 8.2:-16192061 None:intergenic
GTGAACTTTGAAATGGAAGA+CGG 0.572384 8.2:+16192363 MS.gene033046:CDS
CTTACTGCCTGTAGTGGATG+TGG 0.574967 8.2:-16191995 None:intergenic
TGAACTTTGAAATGGAAGAC+GGG 0.575100 8.2:+16192364 MS.gene033046:CDS
ACTGAACTTACTGCCTGTAG+TGG 0.586429 8.2:-16192001 None:intergenic
CTGAGATGACTTCCATCTGT+AGG 0.587562 8.2:-16192309 None:intergenic
TCTTGATTGAAGATGTCCAT+TGG 0.588804 8.2:-16191917 None:intergenic
TCTATCAGACACTCGGCTTG+AGG 0.591247 8.2:-16192268 None:intergenic
TGAAGATGTCCATTGGTGAC+AGG 0.591626 8.2:-16191910 None:intergenic
CTTGCAGATATCAGATTCCA+GGG 0.591867 8.2:+16191778 MS.gene033046:intron
TGCACTCTCACTCTCTTGGG+AGG 0.598164 8.2:-16190288 None:intergenic
TCCTAAGGAGGTGTAGCCAA+TGG 0.598734 8.2:-16192049 None:intergenic
TATCCAGAGGTCTTCCAAAG+TGG 0.601895 8.2:+16191829 MS.gene033046:CDS
TTGTGAATGGAATTGAGCAG+AGG 0.621516 8.2:+16190424 MS.gene033046:CDS
AATTCTGTGGTGGAATCCAA+TGG 0.627306 8.2:+16190147 MS.gene033046:CDS
TGGCAATGCTTCAATTGATG+CGG 0.627501 8.2:+16190215 MS.gene033046:CDS
TAAGAGGCATAAAGTTTGTG+AGG 0.628037 8.2:+16190377 MS.gene033046:CDS
CGACAGTTGTAGTAAATCGA+TGG 0.628450 8.2:-16192448 None:intergenic
GTGATCTTATGCTGTAGACA+TGG 0.639068 8.2:-16192236 None:intergenic
ATAAAAGTCTATCAGACACT+CGG 0.644463 8.2:-16192275 None:intergenic
TCAAGTTTATGGATGTAACA+AGG 0.656151 8.2:+16190329 MS.gene033046:CDS
TTTGTAGAGTGAACGCCTTG+TGG 0.665425 8.2:-16191252 None:intergenic
TAGAGAAAAGATGCTGCTGG+TGG 0.692709 8.2:-16191808 None:intergenic
TAATCCTTGAATGCTTGGTG+CGG 0.726498 8.2:-16192097 None:intergenic
GAGCGTCGAAGAAAACCACA+AGG 0.736819 8.2:+16191237 MS.gene033046:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAATTAATTTAGTAAAA+TGG - chr8.2:16190676-16190695 None:intergenic 10.0%
!!! TGTAAAATTGATTCTATATT+TGG + chr8.2:16190512-16190531 MS.gene033046:intron 15.0%
!! ACTTATTCAACAATAGTTAA+TGG + chr8.2:16191444-16191463 MS.gene033046:intron 20.0%
!! ATTGTTGAATAAGTAACTTA+TGG - chr8.2:16191438-16191457 None:intergenic 20.0%
!! GAAGTAATAAACTTGAAAAT+TGG - chr8.2:16190476-16190495 None:intergenic 20.0%
!! TAGTAACTTAGTTATATGAA+TGG - chr8.2:16191579-16191598 None:intergenic 20.0%
!! TATATATTATTACCACCAAA+TGG - chr8.2:16191297-16191316 None:intergenic 20.0%
!!! AAAATTGATTCTATATTTGG+TGG + chr8.2:16190515-16190534 MS.gene033046:intron 20.0%
!!! AAGCTAATTTTGTTTAAGTT+TGG - chr8.2:16190731-16190750 None:intergenic 20.0%
!!! AGTTATATTTCTGAAGAAAA+AGG + chr8.2:16189973-16189992 MS.gene033046:CDS 20.0%
!!! ATTTTGTTTAAGTTTGGTTT+TGG - chr8.2:16190725-16190744 None:intergenic 20.0%
!!! TAAGAAGCATAATAATCAAT+AGG - chr8.2:16191041-16191060 None:intergenic 20.0%
! AAAACATGTGATCACAATTT+AGG - chr8.2:16190949-16190968 None:intergenic 25.0%
! ATAATAATCAATAGGTGCAA+AGG - chr8.2:16191033-16191052 None:intergenic 25.0%
! ATAGTTATGATATATAGGTC+TGG + chr8.2:16191123-16191142 MS.gene033046:intron 25.0%
! ATTGTAAATAATCACACATG+TGG + chr8.2:16190873-16190892 MS.gene033046:intron 25.0%
! CAAATGAATGTGAAAGAAAA+AGG - chr8.2:16191360-16191379 None:intergenic 25.0%
! GCTGTATAGTTATGATATAT+AGG + chr8.2:16191118-16191137 MS.gene033046:intron 25.0%
! TAATAATCAATAGGTGCAAA+GGG - chr8.2:16191032-16191051 None:intergenic 25.0%
! TCAGATGATTTCAATTTCAT+TGG + chr8.2:16190039-16190058 MS.gene033046:CDS 25.0%
!!! AAAATGGATTATGACTAAAG+AGG - chr8.2:16190660-16190679 None:intergenic 25.0%
!!! CATGTTTTTGTTTTAAACCT+TGG + chr8.2:16190961-16190980 MS.gene033046:intron 25.0%
!!! TAATGTTTAGCTTGTTATAC+AGG + chr8.2:16191148-16191167 MS.gene033046:intron 25.0%
!!! TACATTGATTTCTTTTGTTG+TGG + chr8.2:16191402-16191421 MS.gene033046:intron 25.0%
AATAACCAAAGGGTTATCAA+AGG - chr8.2:16192202-16192221 None:intergenic 30.0%
ACTGCATATTGTCAAGTTTA+TGG + chr8.2:16190318-16190337 MS.gene033046:CDS 30.0%
AGGAATTAAGTATCTCCTTA+TGG + chr8.2:16191474-16191493 MS.gene033046:intron 30.0%
AGGCTAAAAATCAATTGTAG+AGG - chr8.2:16190584-16190603 None:intergenic 30.0%
ATAAAAGTCTATCAGACACT+CGG - chr8.2:16192278-16192297 None:intergenic 30.0%
ATAACCAAAGGGTTATCAAA+GGG - chr8.2:16192201-16192220 None:intergenic 30.0%
ATTCCATTCACAATGACTAT+AGG - chr8.2:16190417-16190436 None:intergenic 30.0%
ATTTCTAACTAGCTGGAATT+TGG + chr8.2:16190550-16190569 MS.gene033046:intron 30.0%
CAATAGTTAATGGTTGAAGA+AGG + chr8.2:16191454-16191473 MS.gene033046:intron 30.0%
CATTCACAAAATCTGAATCA+TGG - chr8.2:16192395-16192414 None:intergenic 30.0%
CGAATGTTGAAAACAAAACA+AGG - chr8.2:16191002-16191021 None:intergenic 30.0%
GAAGTTTATGATTGACCATA+AGG - chr8.2:16191492-16191511 None:intergenic 30.0%
TAGTTATATGAATGGCAACT+CGG - chr8.2:16191571-16191590 None:intergenic 30.0%
TATGATTTGATACGATCCTA+TGG + chr8.2:16191753-16191772 MS.gene033046:intron 30.0%
TCAAGTTTATGGATGTAACA+AGG + chr8.2:16190329-16190348 MS.gene033046:CDS 30.0%
TCATGTTCTAGTATTCCAAT+AGG - chr8.2:16190782-16190801 None:intergenic 30.0%
TCCATCTGTAGGATTTAATT+CGG - chr8.2:16192301-16192320 None:intergenic 30.0%
TGACTAAAGAGGTTAATGTT+TGG - chr8.2:16190649-16190668 None:intergenic 30.0%
TGTAATTTAATGAGTGCAGA+AGG - chr8.2:16191386-16191405 None:intergenic 30.0%
TTATTGTCTGGTACCAATAT+AGG - chr8.2:16192336-16192355 None:intergenic 30.0%
TTCACGATATCATTATTGTC+TGG - chr8.2:16192348-16192367 None:intergenic 30.0%
TTTCAAGCTTTCAAATTCTG+TGG + chr8.2:16190134-16190153 MS.gene033046:CDS 30.0%
! AAGTGAGTTTTCTGTAAAAG+GGG + chr8.2:16190104-16190123 MS.gene033046:CDS 30.0%
! ACTAAAAAAGCATAGGAAAC+TGG - chr8.2:16191712-16191731 None:intergenic 30.0%
! ACTATACAGCTTCTTATTTG+AGG - chr8.2:16191108-16191127 None:intergenic 30.0%
! ATTTTGCATTTCTAACTAGC+TGG + chr8.2:16190543-16190562 MS.gene033046:intron 30.0%
! CAAGTGAGTTTTCTGTAAAA+GGG + chr8.2:16190103-16190122 MS.gene033046:CDS 30.0%
! CTTAGCATCACAATAGTTTT+TGG + chr8.2:16190748-16190767 MS.gene033046:intron 30.0%
! CTTTTACAGAAAACTCACTT+GGG - chr8.2:16190104-16190123 None:intergenic 30.0%
! GCAACATTTTCATGAAGAAA+GGG - chr8.2:16191976-16191995 None:intergenic 30.0%
! TAGGCTTTTGTGAAATGTTT+GGG + chr8.2:16190007-16190026 MS.gene033046:CDS 30.0%
! TCAGATGACTAAAAAAGCAT+AGG - chr8.2:16191719-16191738 None:intergenic 30.0%
! TGATATCGTGAACTTTGAAA+TGG + chr8.2:16192356-16192375 MS.gene033046:CDS 30.0%
! TTAGGCTTTTGTGAAATGTT+TGG + chr8.2:16190006-16190025 MS.gene033046:CDS 30.0%
! TTTTACATGGTACAACACTA+AGG - chr8.2:16191603-16191622 None:intergenic 30.0%
!! AATTGTTTTTCGTTGTACGA+AGG - chr8.2:16191952-16191971 None:intergenic 30.0%
!! GAAAAAGGTTCTGAAGAATT+AGG + chr8.2:16189988-16190007 MS.gene033046:CDS 30.0%
!!! ATGAGCAGGGTTTTAAATTA+TGG + chr8.2:16190825-16190844 MS.gene033046:intron 30.0%
!!! TTGTTTTAAACCTTGGTCTT+GGG + chr8.2:16190968-16190987 MS.gene033046:intron 30.0%
!!! TTTGTTTTAAACCTTGGTCT+TGG + chr8.2:16190967-16190986 MS.gene033046:intron 30.0%
AAGTCATCTCAGTCCTATAT+TGG + chr8.2:16192320-16192339 MS.gene033046:CDS 35.0%
ACTCCTATAGTCATTGTGAA+TGG + chr8.2:16190411-16190430 MS.gene033046:CDS 35.0%
AGGGTTGAGCAATAAATTAG+AGG - chr8.2:16190604-16190623 None:intergenic 35.0%
CACAAACTTTATGCCTCTTA+TGG - chr8.2:16190377-16190396 None:intergenic 35.0%
CTAAGTAGAAACATGACTGA+AGG - chr8.2:16191076-16191095 None:intergenic 35.0%
GACTCACAAGAAGACAAAAT+AGG - chr8.2:16190261-16190280 None:intergenic 35.0%
GTTATCAAAGGGAATTTCCA+AGG - chr8.2:16192190-16192209 None:intergenic 35.0%
GTTGAATTATGTCATGAGCA+GGG + chr8.2:16190812-16190831 MS.gene033046:intron 35.0%
TCCGAATTAAATCCTACAGA+TGG + chr8.2:16192297-16192316 MS.gene033046:CDS 35.0%
TCTTGATTGAAGATGTCCAT+TGG - chr8.2:16191920-16191939 None:intergenic 35.0%
TGTTGAATTATGTCATGAGC+AGG + chr8.2:16190811-16190830 MS.gene033046:intron 35.0%
TTACCATCAAGTTTCTTGCT+TGG - chr8.2:16190072-16190091 None:intergenic 35.0%
TTGCATCATCTTCACAAGAA+AGG - chr8.2:16192428-16192447 None:intergenic 35.0%
! AATTCCCTTTGATAACCCTT+TGG + chr8.2:16192194-16192213 MS.gene033046:CDS 35.0%
! CAAGGATCTTAGTTCTTGTA+AGG + chr8.2:16190347-16190366 MS.gene033046:CDS 35.0%
! CCAAGTGAGTTTTCTGTAAA+AGG + chr8.2:16190102-16190121 MS.gene033046:CDS 35.0%
! CCTTTTACAGAAAACTCACT+TGG - chr8.2:16190105-16190124 None:intergenic 35.0%
! CTAGGAAGATTTGATGATCA+TGG + chr8.2:16190195-16190214 MS.gene033046:CDS 35.0%
! CTTCAGTCATGTTTCTACTT+AGG + chr8.2:16191074-16191093 MS.gene033046:intron 35.0%
! CTTTTTCCCATTTCTAACGA+GGG - chr8.2:16191649-16191668 None:intergenic 35.0%
! GGCAACATTTTCATGAAGAA+AGG - chr8.2:16191977-16191996 None:intergenic 35.0%
! TAAGAGGCATAAAGTTTGTG+AGG + chr8.2:16190377-16190396 MS.gene033046:CDS 35.0%
! TCTTTTTCCCATTTCTAACG+AGG - chr8.2:16191650-16191669 None:intergenic 35.0%
! TTTTCGTTGTACGAAGGAAA+CGG - chr8.2:16191946-16191965 None:intergenic 35.0%
!! GTGAACTTTGAAATGGAAGA+CGG + chr8.2:16192363-16192382 MS.gene033046:CDS 35.0%
!! TCTCTCATTGATTTGAAGCT+AGG + chr8.2:16190177-16190196 MS.gene033046:CDS 35.0%
!! TGAACTTTGAAATGGAAGAC+GGG + chr8.2:16192364-16192383 MS.gene033046:CDS 35.0%
AATGAGAGAATCCCAACCAT+TGG - chr8.2:16190166-16190185 None:intergenic 40.0%
ATGAAAGCAGAACATGAAGC+TGG + chr8.2:16191865-16191884 MS.gene033046:CDS 40.0%
ATTGAAGTCTTCGCTTCCTA+AGG - chr8.2:16192067-16192086 None:intergenic 40.0%
CAAGCTTTCAAATTCTGTGG+TGG + chr8.2:16190137-16190156 MS.gene033046:CDS 40.0%
CACAATGACTATAGGAGTCT+TGG - chr8.2:16190409-16190428 None:intergenic 40.0%
CCAAGCATTCAAGGATTATC+AGG + chr8.2:16192102-16192121 MS.gene033046:CDS 40.0%
CCTGATAATCCTTGAATGCT+TGG - chr8.2:16192105-16192124 None:intergenic 40.0%
CGACAGTTGTAGTAAATCGA+TGG - chr8.2:16192451-16192470 None:intergenic 40.0%
CTGATATCTGCAAGAACCAT+AGG - chr8.2:16191772-16191791 None:intergenic 40.0%
CTTGCAGATATCAGATTCCA+GGG + chr8.2:16191778-16191797 MS.gene033046:intron 40.0%
CTTGTAAGGACTACCATAAG+AGG + chr8.2:16190361-16190380 MS.gene033046:CDS 40.0%
GCATCATAGACAATCATTGC+AGG + chr8.2:16192491-16192510 MS.gene033046:CDS 40.0%
GCGTTCACTCTACAAATTCT+GGG + chr8.2:16191259-16191278 MS.gene033046:CDS 40.0%
GCTTCATGTTCTGCTTTCAT+AGG - chr8.2:16191865-16191884 None:intergenic 40.0%
GTGATCTTATGCTGTAGACA+TGG - chr8.2:16192239-16192258 None:intergenic 40.0%
GTTCCAAGCAAGAAACTTGA+TGG + chr8.2:16190066-16190085 MS.gene033046:CDS 40.0%
TCTTGCAGATATCAGATTCC+AGG + chr8.2:16191777-16191796 MS.gene033046:intron 40.0%
TGAATGGCAACTCGGAATTT+AGG - chr8.2:16191563-16191582 None:intergenic 40.0%
TGGCAATGCTTCAATTGATG+CGG + chr8.2:16190215-16190234 MS.gene033046:CDS 40.0%
TTATGCTGTAGACATGGCTA+TGG - chr8.2:16192233-16192252 None:intergenic 40.0%
TTGCTGTCTGAGTTTGATGA+TGG + chr8.2:16191177-16191196 MS.gene033046:CDS 40.0%
TTGTCAACAATGTAGCAGGT+TGG + chr8.2:16190449-16190468 MS.gene033046:intron 40.0%
TTGTGAATGGAATTGAGCAG+AGG + chr8.2:16190424-16190443 MS.gene033046:CDS 40.0%
! AATTCTGTGGTGGAATCCAA+TGG + chr8.2:16190147-16190166 MS.gene033046:CDS 40.0%
! AGCATCTTTTCTCTATCCAG+AGG + chr8.2:16191816-16191835 MS.gene033046:CDS 40.0%
! ATTGATGCGGCATTTTCCAA+AGG + chr8.2:16190228-16190247 MS.gene033046:CDS 40.0%
! GTTTTCTTCGACGCTCATTA+TGG - chr8.2:16191233-16191252 None:intergenic 40.0%
! TAATCCTTGAATGCTTGGTG+CGG - chr8.2:16192100-16192119 None:intergenic 40.0%
!! GGTTTTGTCAACAATGTAGC+AGG + chr8.2:16190445-16190464 MS.gene033046:CDS 40.0%
!! TTTTTGGTGTGTGTGCCTAT+TGG + chr8.2:16190764-16190783 MS.gene033046:intron 40.0%
AATGTTGCCACATCCACTAC+AGG + chr8.2:16191988-16192007 MS.gene033046:CDS 45.0%
ACTGAACTTACTGCCTGTAG+TGG - chr8.2:16192004-16192023 None:intergenic 45.0%
AGACTGTTTCAGCCATTTGG+TGG + chr8.2:16191282-16191301 MS.gene033046:CDS 45.0%
AGCCAATGGCATGAGCATAA+TGG - chr8.2:16192038-16192057 None:intergenic 45.0%
AGCCATTATGCTCATGCCAT+TGG + chr8.2:16192033-16192052 MS.gene033046:CDS 45.0%
AGGTCTTCCAAAGTGGTTTC+TGG + chr8.2:16191836-16191855 MS.gene033046:CDS 45.0%
CAAAGGGAATTTCCAAGGAC+TGG - chr8.2:16192185-16192204 None:intergenic 45.0%
CATGTAAAATGCCGCTGACA+CGG + chr8.2:16191614-16191633 MS.gene033046:intron 45.0%
CTGAGATGACTTCCATCTGT+AGG - chr8.2:16192312-16192331 None:intergenic 45.0%
GGATAGAGAAAAGATGCTGC+TGG - chr8.2:16191814-16191833 None:intergenic 45.0%
GGCGTTCACTCTACAAATTC+TGG + chr8.2:16191258-16191277 MS.gene033046:CDS 45.0%
GGCTGCTTGGAATAACCAAA+GGG - chr8.2:16192212-16192231 None:intergenic 45.0%
TAACACTTCAAGCCAGTCCT+TGG + chr8.2:16192170-16192189 MS.gene033046:CDS 45.0%
TAGAGAAAAGATGCTGCTGG+TGG - chr8.2:16191811-16191830 None:intergenic 45.0%
TAGGTCTCCAGAAACCACTT+TGG - chr8.2:16191846-16191865 None:intergenic 45.0%
TATCCAGAGGTCTTCCAAAG+TGG + chr8.2:16191829-16191848 MS.gene033046:CDS 45.0%
TATGGCCTGCAAGTCGTTTA+CGG - chr8.2:16191215-16191234 None:intergenic 45.0%
TGGCTGCTTGGAATAACCAA+AGG - chr8.2:16192213-16192232 None:intergenic 45.0%
TTCCCATTTCTAACGAGGGA+CGG - chr8.2:16191645-16191664 None:intergenic 45.0%
TTCCGTCCCTCGTTAGAAAT+GGG + chr8.2:16191640-16191659 MS.gene033046:intron 45.0%
TTTCCGTCCCTCGTTAGAAA+TGG + chr8.2:16191639-16191658 MS.gene033046:intron 45.0%
TTTGTAGAGTGAACGCCTTG+TGG - chr8.2:16191255-16191274 None:intergenic 45.0%
! AAACCACTTTGGAAGACCTC+TGG - chr8.2:16191835-16191854 None:intergenic 45.0%
! TGAAGATGTCCATTGGTGAC+AGG - chr8.2:16191913-16191932 None:intergenic 45.0%
! TGTGGTGGAATCCAATGGTT+GGG + chr8.2:16190152-16190171 MS.gene033046:CDS 45.0%
!! CAAAATAGGTGCTGCTCCTT+TGG - chr8.2:16190247-16190266 None:intergenic 45.0%
!! CAGAACATGAAGCTGGCTTT+AGG + chr8.2:16191872-16191891 MS.gene033046:CDS 45.0%
!! GGACTGGCTTGAAGTGTTAT+TGG - chr8.2:16192169-16192188 None:intergenic 45.0%
!! ACAATTATTATATAAAAAAA+AGG - chr8.2:16190624-16190643 None:intergenic 5.0%
!! CAATTATTATATAAAAAAAA+GGG - chr8.2:16190623-16190642 None:intergenic 5.0%
AAAAGATGCTGCTGGTGGCT+TGG - chr8.2:16191806-16191825 None:intergenic 50.0%
AGTTGCCGTAAACGACTTGC+AGG + chr8.2:16191207-16191226 MS.gene033046:CDS 50.0%
CATACCGCACCAAGCATTCA+AGG + chr8.2:16192093-16192112 MS.gene033046:CDS 50.0%
GAAGTCTTCGCTTCCTAAGG+AGG - chr8.2:16192064-16192083 None:intergenic 50.0%
GAGTGCACTCTCACTCTCTT+GGG - chr8.2:16190294-16190313 None:intergenic 50.0%
GGCTTCAAAACCCAAGACCA+AGG - chr8.2:16190981-16191000 None:intergenic 50.0%
GGGAGACTGTTTCAGCCATT+TGG + chr8.2:16191279-16191298 MS.gene033046:CDS 50.0%
TAGACATGGCTATGGCTGCT+TGG - chr8.2:16192225-16192244 None:intergenic 50.0%
TCTTCTGTGCCTGTCACCAA+TGG + chr8.2:16191901-16191920 MS.gene033046:CDS 50.0%
TGAGTGCACTCTCACTCTCT+TGG - chr8.2:16190295-16190314 None:intergenic 50.0%
! CGTGTCAGCGGCATTTTACA+TGG - chr8.2:16191616-16191635 None:intergenic 50.0%
! CTGTGGTGGAATCCAATGGT+TGG + chr8.2:16190151-16190170 MS.gene033046:CDS 50.0%
! TCCTAAGGAGGTGTAGCCAA+TGG - chr8.2:16192052-16192071 None:intergenic 50.0%
! TCTATCAGACACTCGGCTTG+AGG - chr8.2:16192271-16192290 None:intergenic 50.0%
!! CTTACTGCCTGTAGTGGATG+TGG - chr8.2:16191998-16192017 None:intergenic 50.0%
!! GAGCGTCGAAGAAAACCACA+AGG + chr8.2:16191237-16191256 MS.gene033046:CDS 50.0%
GGACGGAAAATCCGTGTCAG+CGG - chr8.2:16191628-16191647 None:intergenic 55.0%
TGCACTCTCACTCTCTTGGG+AGG - chr8.2:16190291-16190310 None:intergenic 55.0%
! GCCATTGGCTACACCTCCTT+AGG + chr8.2:16192048-16192067 MS.gene033046:CDS 55.0%
!! CTGCTGGTGGCTTGGTTCCC+TGG - chr8.2:16191798-16191817 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 16189961 16192557 16189961 ID=MS.gene033046
chr8.2 mRNA 16189961 16192557 16189961 ID=MS.gene033046.t1;Parent=MS.gene033046
chr8.2 exon 16189961 16190466 16189961 ID=MS.gene033046.t1.exon1;Parent=MS.gene033046.t1
chr8.2 CDS 16189961 16190466 16189961 ID=cds.MS.gene033046.t1;Parent=MS.gene033046.t1
chr8.2 exon 16191170 16191303 16191170 ID=MS.gene033046.t1.exon2;Parent=MS.gene033046.t1
chr8.2 CDS 16191170 16191303 16191170 ID=cds.MS.gene033046.t1;Parent=MS.gene033046.t1
chr8.2 exon 16191785 16192557 16191785 ID=MS.gene033046.t1.exon3;Parent=MS.gene033046.t1
chr8.2 CDS 16191785 16192557 16191785 ID=cds.MS.gene033046.t1;Parent=MS.gene033046.t1
Gene Sequence

>MS.gene033046

ATGGAATCTTGGAGTTATATTTCTGAAGAAAAAGGTTCTGAAGAATTAGGCTTTTGTGAAATGTTTGGGAAACAATATTCAGATGATTTCAATTTCATTGGAAGTGTTCCAAGCAAGAAACTTGATGGTAAATTTGATGACCCAAGTGAGTTTTCTGTAAAAGGGGACACAAATTTCAAGCTTTCAAATTCTGTGGTGGAATCCAATGGTTGGGATTCTCTCATTGATTTGAAGCTAGGAAGATTTGATGATCATGGCAATGCTTCAATTGATGCGGCATTTTCCAAAGGAGCAGCACCTATTTTGTCTTCTTGTGAGTCTTCAACACCTCCCAAGAGAGTGAGAGTGCACTCAATGACTGCATATTGTCAAGTTTATGGATGTAACAAGGATCTTAGTTCTTGTAAGGACTACCATAAGAGGCATAAAGTTTGTGAGGTTCACTCCAAGACTCCTATAGTCATTGTGAATGGAATTGAGCAGAGGTTTTGTCAACAATGTAGCAGGTTGGTCCAATTTTCAAGTTTATTACTTCTTTCAATTCATTTTAGTGTAAAATTGATTCTATATTTGGTGGAATTGATTTTGCATTTCTAACTAGCTGGAATTTGGAATTGTTGCCTCTACAATTGATTTTTAGCCTCTAATTTATTGCTCAACCCTTTTTTTTATATAATAATTGTATCCAAACATTAACCTCTTTAGTCATAATCCATTTTACTAAATTAATTCATTCAAAATTAATTTTTGTTATTGCAGAACCAAAACCAAACTTAAACAAAATTAGCTTAGCATCACAATAGTTTTTGGTGTGTGTGCCTATTGGAATACTAGAACATGAATCTTTACTTGTTGAATTATGTCATGAGCAGGGTTTTAAATTATGGTTGTGTTGCAATAATTGAAATTAATATTGTAAATAATCACACATGTGGCTTATTTGATTTTAATTATCACTGCGAAATACATCGAAAACTTTAATGTGCCTAAATTGTGATCACATGTTTTTGTTTTAAACCTTGGTCTTGGGTTTTGAAGCCTTGTTTTGTTTTCAACATTCGTTGCTTACCCTTTGCACCTATTGATTATTATGCTTCTTAAATGAAGTTTTTCCTTCAGTCATGTTTCTACTTAGGATGATAAGCCTCAAATAAGAAGCTGTATAGTTATGATATATAGGTCTGGTGTAATGTTTAGCTTGTTATACAGGTTTCATTTGCTGTCTGAGTTTGATGATGGTAAGCGCAGTTGCCGTAAACGACTTGCAGGCCATAATGAGCGTCGAAGAAAACCACAAGGCGTTCACTCTACAAATTCTGGGAGACTGTTTCAGCCATTTGGTGGTAATAATATATATAATAAAGTTAGATCATGATTTAACTTTATTCTTTACACTCCTTTTTCTTTCACATTCATTTGTTGCCTTCTGCACTCATTAAATTACATTGATTTCTTTTGTTGTGGTTGATGTGATCCATAAGTTACTTATTCAACAATAGTTAATGGTTGAAGAAGGAATTAAGTATCTCCTTATGGTCAATCATAAACTTCTATCTGTTTTCTTACAGAATGACATCTAAATCTAATTAATTGTAGTTACCTAAATTCCGAGTTGCCATTCATATAACTAAGTTACTAACCTTAGTGTTGTACCATGTAAAATGCCGCTGACACGGATTTTCCGTCCCTCGTTAGAAATGGGAAAAAGATGAAGCATTGTGACTCTTATAATAATTGATTATCTGAAGCCAGTTTCCTATGCTTTTTTAGTCATCTGATATTAATATAAATGTATGATTTGATACGATCCTATGGTTCTTGCAGATATCAGATTCCAGGGAACCAAGCCACCAGCAGCATCTTTTCTCTATCCAGAGGTCTTCCAAAGTGGTTTCTGGAGACCTATGAAAGCAGAACATGAAGCTGGCTTTAGGCATTTTTCTTCTGTGCCTGTCACCAATGGACATCTTCAATCAAGATCTCCGTTTCCTTCGTACAACGAAAAACAATTTCCCTTTCTTCATGAAAATGTTGCCACATCCACTACAGGCAGTAAGTTCAGTGAAAACAATAGCCATTATGCTCATGCCATTGGCTACACCTCCTTAGGAAGCGAAGACTTCAATGTTTTTCATACCGCACCAAGCATTCAAGGATTATCAGGAATTTCAGACTCTTGTGCTCTCTCTCTTCTGTCATCTCAGTCCAATAACACTTCAAGCCAGTCCTTGGAAATTCCCTTTGATAACCCTTTGGTTATTCCAAGCAGCCATAGCCATGTCTACAGCATAAGATCACAAGCGACTTCCTCAAGCCGAGTGTCTGATAGACTTTTATCCGAATTAAATCCTACAGATGGAAGTCATCTCAGTCCTATATTGGTACCAGACAATAATGATATCGTGAACTTTGAAATGGAAGACGGGATCTTCCATGATTCAGATTTTGTGAATGTGAAAGACTGCCTTTCTTGTGAAGATGATGCAACCATCGATTTACTACAACTGTCGTCGCAGCTTCAACGAGTTGAGCATCATAGACAATCATTGCAGGTGAAGAAAGAGTTTGATTCTTCTTGCACTCTCCGAATTACTTAA
Protein sequence

>MS.gene033046.t1

MESWSYISEEKGSEELGFCEMFGKQYSDDFNFIGSVPSKKLDGKFDDPSEFSVKGDTNFKLSNSVVESNGWDSLIDLKLGRFDDHGNASIDAAFSKGAAPILSSCESSTPPKRVRVHSMTAYCQVYGCNKDLSSCKDYHKRHKVCEVHSKTPIVIVNGIEQRFCQQCSRFHLLSEFDDGKRSCRKRLAGHNERRRKPQGVHSTNSGRLFQPFGDIRFQGTKPPAASFLYPEVFQSGFWRPMKAEHEAGFRHFSSVPVTNGHLQSRSPFPSYNEKQFPFLHENVATSTTGSKFSENNSHYAHAIGYTSLGSEDFNVFHTAPSIQGLSGISDSCALSLLSSQSNNTSSQSLEIPFDNPLVIPSSHSHVYSIRSQATSSSRVSDRLLSELNPTDGSHLSPILVPDNNDIVNFEMEDGIFHDSDFVNVKDCLSCEDDATIDLLQLSSQLQRVEHHRQSLQVKKEFDSSCTLRIT