Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033123.t1 | RHN63663.1 | 100 | 55 | 0 | 0 | 22 | 76 | 23 | 77 | 3.90E-25 | 123.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033123.t1 | Q2Q493 | 70.3 | 74 | 18 | 2 | 1 | 71 | 1 | 73 | 3.2e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033123.t1 | A0A396IG90 | 100.0 | 55 | 0 | 0 | 22 | 76 | 23 | 77 | 2.8e-25 | 123.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene033123.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033123.t1 | MTR_3g039810 | 70.270 | 74 | 15 | 3 | 1 | 71 | 1 | 70 | 1.51e-27 | 95.5 |
| MS.gene033123.t1 | MTR_6g012220 | 69.565 | 69 | 17 | 2 | 1 | 69 | 1 | 65 | 1.12e-26 | 93.2 |
| MS.gene033123.t1 | MTR_3g100650 | 62.264 | 53 | 17 | 1 | 22 | 71 | 78 | 130 | 1.43e-16 | 71.6 |
| MS.gene033123.t1 | MTR_8g467340 | 57.627 | 59 | 20 | 1 | 17 | 70 | 57 | 115 | 3.94e-16 | 71.2 |
| MS.gene033123.t1 | MTR_1g077020 | 60.000 | 55 | 17 | 2 | 21 | 71 | 6 | 59 | 6.93e-16 | 68.6 |
| MS.gene033123.t1 | MTR_5g065440 | 60.377 | 53 | 18 | 1 | 22 | 71 | 56 | 108 | 4.03e-15 | 67.4 |
| MS.gene033123.t1 | MTR_7g010300 | 55.556 | 54 | 19 | 1 | 22 | 70 | 86 | 139 | 4.50e-15 | 68.2 |
| MS.gene033123.t1 | MTR_2g076780 | 50.000 | 66 | 27 | 2 | 8 | 70 | 24 | 86 | 7.46e-15 | 66.6 |
| MS.gene033123.t1 | MTR_4g134580 | 59.649 | 57 | 18 | 2 | 24 | 76 | 113 | 168 | 3.82e-14 | 65.5 |
| MS.gene033123.t1 | MTR_8g069080 | 56.000 | 50 | 19 | 1 | 24 | 70 | 69 | 118 | 7.58e-14 | 64.3 |
| MS.gene033123.t1 | MTR_5g020510 | 52.459 | 61 | 24 | 2 | 17 | 73 | 92 | 151 | 1.07e-13 | 63.9 |
| MS.gene033123.t1 | MTR_5g032600 | 58.000 | 50 | 18 | 1 | 24 | 70 | 78 | 127 | 1.72e-13 | 63.2 |
| MS.gene033123.t1 | MTR_6g012280 | 59.615 | 52 | 16 | 2 | 24 | 70 | 91 | 142 | 1.96e-13 | 63.5 |
| MS.gene033123.t1 | MTR_6g073013 | 56.000 | 50 | 18 | 1 | 24 | 69 | 19 | 68 | 2.15e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033123.t1 | AT1G18835 | 64.384 | 73 | 24 | 1 | 1 | 71 | 1 | 73 | 4.71e-26 | 91.7 |
| MS.gene033123.t1 | AT3G28917 | 55.952 | 84 | 24 | 2 | 1 | 71 | 1 | 84 | 4.93e-23 | 84.3 |
| MS.gene033123.t1 | AT1G74660 | 69.811 | 53 | 13 | 1 | 22 | 71 | 37 | 89 | 4.10e-19 | 74.7 |
| MS.gene033123.t1 | AT1G74660 | 69.811 | 53 | 13 | 1 | 22 | 71 | 37 | 89 | 5.89e-19 | 73.9 |
| MS.gene033123.t1 | AT3G50890 | 52.778 | 72 | 27 | 3 | 6 | 71 | 40 | 110 | 1.91e-16 | 70.9 |
| MS.gene033123.t1 | AT1G14440 | 59.649 | 57 | 18 | 2 | 19 | 71 | 85 | 140 | 6.69e-16 | 70.1 |
| MS.gene033123.t1 | AT1G14440 | 59.649 | 57 | 18 | 2 | 19 | 71 | 85 | 140 | 6.69e-16 | 70.1 |
| MS.gene033123.t1 | AT2G18350 | 60.784 | 51 | 17 | 1 | 24 | 71 | 82 | 132 | 1.18e-15 | 68.9 |
| MS.gene033123.t1 | AT2G02540 | 61.111 | 54 | 16 | 2 | 21 | 70 | 84 | 136 | 1.65e-15 | 68.9 |
| MS.gene033123.t1 | AT2G02540 | 61.111 | 54 | 16 | 2 | 21 | 70 | 84 | 136 | 1.65e-15 | 68.9 |
| MS.gene033123.t1 | AT5G15210 | 56.364 | 55 | 19 | 1 | 21 | 70 | 53 | 107 | 2.54e-15 | 68.2 |
| MS.gene033123.t1 | AT5G42780 | 60.000 | 50 | 19 | 1 | 24 | 72 | 64 | 113 | 6.32e-15 | 66.6 |
| MS.gene033123.t1 | AT4G24660 | 61.111 | 54 | 16 | 2 | 22 | 71 | 47 | 99 | 7.21e-15 | 66.2 |
| MS.gene033123.t1 | AT5G39760 | 50.000 | 66 | 28 | 1 | 9 | 69 | 41 | 106 | 9.51e-15 | 67.0 |
| MS.gene033123.t1 | AT4G24660 | 61.111 | 54 | 16 | 2 | 22 | 71 | 47 | 99 | 1.07e-14 | 65.9 |
| MS.gene033123.t1 | AT1G69600 | 58.491 | 53 | 17 | 1 | 22 | 69 | 29 | 81 | 2.27e-14 | 65.5 |
| MS.gene033123.t1 | AT3G28920 | 55.932 | 59 | 21 | 1 | 22 | 75 | 50 | 108 | 3.18e-14 | 65.5 |
| MS.gene033123.t1 | AT5G60480 | 51.852 | 54 | 21 | 1 | 21 | 69 | 34 | 87 | 8.32e-14 | 63.5 |
| MS.gene033123.t1 | AT5G65410 | 56.863 | 51 | 19 | 1 | 24 | 71 | 75 | 125 | 1.06e-13 | 63.9 |
| MS.gene033123.t1 | AT1G75240 | 52.542 | 59 | 25 | 1 | 16 | 71 | 68 | 126 | 1.82e-13 | 63.5 |
| MS.gene033123.t1 | AT1G14687 | 52.174 | 46 | 20 | 1 | 24 | 69 | 7 | 50 | 8.58e-11 | 54.7 |
Find 15 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAGAAATATTGTTGGATA+TGG | 0.243499 | 8.2:-17333327 | MS.gene033123:CDS |
| ATGGAATTCATGGCATGTTC+AGG | 0.335777 | 8.2:-17333249 | MS.gene033123:CDS |
| TCATCTTCAAGAAATATTGT+TGG | 0.392599 | 8.2:-17333333 | MS.gene033123:CDS |
| GTGCTGCTTGCGGTTGTCAT+AGG | 0.419461 | 8.2:-17333209 | MS.gene033123:CDS |
| GTCATAGGAACTTCCACAAA+AGG | 0.480871 | 8.2:-17333194 | MS.gene033123:CDS |
| CTATGCTGTTGATGGATGTA+TGG | 0.485307 | 8.2:-17333268 | MS.gene033123:CDS |
| AAGAATCATGCAGCATATAG+TGG | 0.485623 | 8.2:-17333294 | MS.gene033123:CDS |
| AGTGGAGGCTATGCTGTTGA+TGG | 0.489516 | 8.2:-17333276 | MS.gene033123:CDS |
| TGATGGATGTATGGAATTCA+TGG | 0.495255 | 8.2:-17333259 | MS.gene033123:CDS |
| TGGAATTCATGGCATGTTCA+GGG | 0.512543 | 8.2:-17333248 | MS.gene033123:CDS |
| AAAACGGCAAGTGGTGGTGG+TGG | 0.605250 | 8.2:-17333373 | MS.gene033123:CDS |
| GCACTTACATGTGCTGCTTG+CGG | 0.620281 | 8.2:-17333219 | MS.gene033123:CDS |
| GGAATTCATGGCATGTTCAG+GGG | 0.647724 | 8.2:-17333247 | MS.gene033123:CDS |
| AATCATGCAGCATATAGTGG+AGG | 0.667408 | 8.2:-17333291 | MS.gene033123:CDS |
| GTATTGCAAAGTACAGACTG+AGG | 0.757439 | 8.2:-17333168 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCAAGAAATATTGTTGGATA+TGG | - | chr8.2:17333221-17333240 | MS.gene033123:CDS | 25.0% |
| ! | TCATCTTCAAGAAATATTGT+TGG | - | chr8.2:17333215-17333234 | MS.gene033123:CDS | 25.0% |
| ! | TTTGCAATACTTTCCTTTTG+TGG | + | chr8.2:17333370-17333389 | None:intergenic | 30.0% |
| AAGAATCATGCAGCATATAG+TGG | - | chr8.2:17333254-17333273 | MS.gene033123:CDS | 35.0% | |
| ! | TGATGGATGTATGGAATTCA+TGG | - | chr8.2:17333289-17333308 | MS.gene033123:CDS | 35.0% |
| AATCATGCAGCATATAGTGG+AGG | - | chr8.2:17333257-17333276 | MS.gene033123:CDS | 40.0% | |
| ATGGAATTCATGGCATGTTC+AGG | - | chr8.2:17333299-17333318 | MS.gene033123:CDS | 40.0% | |
| GTCATAGGAACTTCCACAAA+AGG | - | chr8.2:17333354-17333373 | MS.gene033123:CDS | 40.0% | |
| TGGAATTCATGGCATGTTCA+GGG | - | chr8.2:17333300-17333319 | MS.gene033123:CDS | 40.0% | |
| ! | CTATGCTGTTGATGGATGTA+TGG | - | chr8.2:17333280-17333299 | MS.gene033123:CDS | 40.0% |
| GGAATTCATGGCATGTTCAG+GGG | - | chr8.2:17333301-17333320 | MS.gene033123:CDS | 45.0% | |
| AGTGGAGGCTATGCTGTTGA+TGG | - | chr8.2:17333272-17333291 | MS.gene033123:CDS | 50.0% | |
| GAAAAAACGGCAAGTGGTGG+TGG | - | chr8.2:17333172-17333191 | MS.gene033123:CDS | 50.0% | |
| !! | GCACTTACATGTGCTGCTTG+CGG | - | chr8.2:17333329-17333348 | MS.gene033123:CDS | 50.0% |
| !! | AAAACGGCAAGTGGTGGTGG+TGG | - | chr8.2:17333175-17333194 | MS.gene033123:CDS | 55.0% |
| !! | GTGCTGCTTGCGGTTGTCAT+AGG | - | chr8.2:17333339-17333358 | MS.gene033123:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 17333170 | 17333400 | 17333170 | ID=MS.gene033123 |
| chr8.2 | mRNA | 17333170 | 17333400 | 17333170 | ID=MS.gene033123.t1;Parent=MS.gene033123 |
| chr8.2 | exon | 17333170 | 17333400 | 17333170 | ID=MS.gene033123.t1.exon1;Parent=MS.gene033123.t1 |
| chr8.2 | CDS | 17333170 | 17333400 | 17333170 | ID=cds.MS.gene033123.t1;Parent=MS.gene033123.t1 |
| Gene Sequence |
| Protein sequence |
