Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033178.t1 | XP_013457865.1 | 97.4 | 233 | 6 | 0 | 1 | 233 | 67 | 299 | 3.70E-103 | 384.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033178.t1 | Q9STS6 | 25.9 | 239 | 131 | 5 | 1 | 196 | 93 | 328 | 7.3e-07 | 55.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033178.t1 | A0A072URD2 | 97.4 | 233 | 6 | 0 | 1 | 233 | 67 | 299 | 2.7e-103 | 384.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene033178.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033178.t1 | MTR_4g107450 | 98.712 | 233 | 3 | 0 | 1 | 233 | 67 | 299 | 9.78e-173 | 477 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033178.t1 | AT3G47870 | 30.417 | 240 | 119 | 8 | 1 | 196 | 93 | 328 | 1.03e-16 | 78.2 |
Find 41 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCATTCAAGCTAATTAT+AGG | 0.238366 | 8.3:+16288527 | MS.gene033178:CDS |
| GTGACTTCACAGTTAGAATT+AGG | 0.247178 | 8.3:+16288697 | MS.gene033178:CDS |
| ATCAACAACTTGAAATTTGC+AGG | 0.281961 | 8.3:+16288638 | MS.gene033178:CDS |
| GCAGGCTACAATATCAAATA+TGG | 0.297786 | 8.3:+16288587 | MS.gene033178:CDS |
| AGCAATGCAACCTCATAAAT+TGG | 0.298388 | 8.3:+16288957 | MS.gene033178:CDS |
| GCAATCATATATTTATTCTA+AGG | 0.300515 | 8.3:+16289077 | MS.gene033178:CDS |
| TATCATTCAAGCTAATTATA+GGG | 0.334002 | 8.3:+16288528 | MS.gene033178:CDS |
| GGGCACTGAAGCCACAAATT+AGG | 0.358880 | 8.3:-16288907 | None:intergenic |
| TATCTTCAACAACTCCTTGT+TGG | 0.395214 | 8.3:-16289011 | None:intergenic |
| CTGCTATTGTTATTTGTGTA+AGG | 0.395645 | 8.3:-16288928 | None:intergenic |
| CATCATGAGGTAACTGTTGT+TGG | 0.408383 | 8.3:-16288672 | None:intergenic |
| TTGTTATAAGTCTGTGGTTG+TGG | 0.430309 | 8.3:-16288766 | None:intergenic |
| AAAGAGTGCGAGCGCATTGA+AGG | 0.447294 | 8.3:-16288734 | None:intergenic |
| ATACTACAAATCCTAATTTG+TGG | 0.454012 | 8.3:+16288896 | MS.gene033178:CDS |
| GATGTTGTGAGGAAATTTGC+AGG | 0.467829 | 8.3:+16288569 | MS.gene033178:CDS |
| TCTGAGGTAACCAATTTATG+AGG | 0.490694 | 8.3:-16288967 | None:intergenic |
| ACAATATCAAATATGGATGA+TGG | 0.492612 | 8.3:+16288594 | MS.gene033178:CDS |
| TGCTATTGTTATTTGTGTAA+GGG | 0.508900 | 8.3:-16288927 | None:intergenic |
| ATAATCCACACTATTGCTGT+TGG | 0.518450 | 8.3:-16288839 | None:intergenic |
| TAATAACTCCTCCCTCTACA+TGG | 0.525658 | 8.3:+16288861 | MS.gene033178:CDS |
| TTGAATGATAATAGAATCCA+TGG | 0.528060 | 8.3:-16288515 | None:intergenic |
| CTCCCTCTACATGGATTCAA+AGG | 0.529912 | 8.3:+16288870 | MS.gene033178:CDS |
| ATCATATATTTATTCTAAGG+AGG | 0.550257 | 8.3:+16289080 | MS.gene033178:CDS |
| CTGATCAGTTGACACTGGTA+AGG | 0.557706 | 8.3:-16289486 | None:intergenic |
| ATCCTTTGAATCCATGTAGA+GGG | 0.563213 | 8.3:-16288872 | None:intergenic |
| TTATTGTTGTTATAAGTCTG+TGG | 0.569885 | 8.3:-16288772 | None:intergenic |
| TCCTCACAACATCCATGCAC+AGG | 0.573785 | 8.3:-16288559 | None:intergenic |
| CACACTATTGCTGTTGGAGT+AGG | 0.575688 | 8.3:-16288833 | None:intergenic |
| CCTTGTTGGACAGATGATAG+TGG | 0.578651 | 8.3:-16288997 | None:intergenic |
| AGGGATAAGTATCCTGTGCA+TGG | 0.583412 | 8.3:+16288547 | MS.gene033178:CDS |
| TTCACAGTTAGAATTAGGAA+TGG | 0.586596 | 8.3:+16288702 | MS.gene033178:CDS |
| CATCCTTTGAATCCATGTAG+AGG | 0.619080 | 8.3:-16288873 | None:intergenic |
| CTACTCCAACAGCAATAGTG+TGG | 0.620940 | 8.3:+16288834 | MS.gene033178:CDS |
| CCACTATCATCTGTCCAACA+AGG | 0.637452 | 8.3:+16288997 | MS.gene033178:CDS |
| TGATAGTGGCTGCGACTCTG+AGG | 0.643276 | 8.3:-16288983 | None:intergenic |
| CTAAGGAGGCTTATGAATCG+AGG | 0.657710 | 8.3:+16289094 | MS.gene033178:CDS |
| TAGTACTGATCAGTTGACAC+TGG | 0.662425 | 8.3:-16289491 | None:intergenic |
| CTTTGAATCCATGTAGAGGG+AGG | 0.666923 | 8.3:-16288869 | None:intergenic |
| TGTGAAGTCACATCATCATG+AGG | 0.679884 | 8.3:-16288685 | None:intergenic |
| TCCTGTGCATGGATGTTGTG+AGG | 0.692042 | 8.3:+16288558 | MS.gene033178:CDS |
| TGTTGCTGAGATACAGACAA+AGG | 0.703585 | 8.3:-16288808 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGAAATTAGATATAG+TGG | + | chr8.3:16289293-16289312 | MS.gene033178:intron | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | + | chr8.3:16289294-16289313 | MS.gene033178:intron | 20.0% |
| !! | GCAATCATATATTTATTCTA+AGG | + | chr8.3:16289077-16289096 | MS.gene033178:CDS | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | + | chr8.3:16288528-16288547 | MS.gene033178:CDS | 20.0% |
| !! | TTATCATTCAAGCTAATTAT+AGG | + | chr8.3:16288527-16288546 | MS.gene033178:CDS | 20.0% |
| !!! | ATCATATATTTATTCTAAGG+AGG | + | chr8.3:16289080-16289099 | MS.gene033178:CDS | 20.0% |
| ! | ACAATATCAAATATGGATGA+TGG | + | chr8.3:16288594-16288613 | MS.gene033178:CDS | 25.0% |
| ! | ATACTACAAATCCTAATTTG+TGG | + | chr8.3:16288896-16288915 | MS.gene033178:CDS | 25.0% |
| !! | TTATTGTTGTTATAAGTCTG+TGG | - | chr8.3:16288775-16288794 | None:intergenic | 25.0% |
| !!! | GTGTTTTTGATCTACTTTAA+CGG | - | chr8.3:16289158-16289177 | None:intergenic | 25.0% |
| !!! | TGCTATTGTTATTTGTGTAA+GGG | - | chr8.3:16288930-16288949 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | + | chr8.3:16289055-16289074 | MS.gene033178:CDS | 25.0% |
| ATCAACAACTTGAAATTTGC+AGG | + | chr8.3:16288638-16288657 | MS.gene033178:CDS | 30.0% | |
| TTCACAGTTAGAATTAGGAA+TGG | + | chr8.3:16288702-16288721 | MS.gene033178:CDS | 30.0% | |
| ! | TATCAGCATTTTCACTACAA+AGG | - | chr8.3:16289354-16289373 | None:intergenic | 30.0% |
| !! | CTGCTATTGTTATTTGTGTA+AGG | - | chr8.3:16288931-16288950 | None:intergenic | 30.0% |
| AGCAATGCAACCTCATAAAT+TGG | + | chr8.3:16288957-16288976 | MS.gene033178:CDS | 35.0% | |
| ATAATCCACACTATTGCTGT+TGG | - | chr8.3:16288842-16288861 | None:intergenic | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | - | chr8.3:16288875-16288894 | None:intergenic | 35.0% | |
| GCAGGCTACAATATCAAATA+TGG | + | chr8.3:16288587-16288606 | MS.gene033178:CDS | 35.0% | |
| GCTTATGAATCGAGGTAAAT+TGG | + | chr8.3:16289102-16289121 | MS.gene033178:intron | 35.0% | |
| GTGACTTCACAGTTAGAATT+AGG | + | chr8.3:16288697-16288716 | MS.gene033178:CDS | 35.0% | |
| TATCTTCAACAACTCCTTGT+TGG | - | chr8.3:16289014-16289033 | None:intergenic | 35.0% | |
| TTGTTATAAGTCTGTGGTTG+TGG | - | chr8.3:16288769-16288788 | None:intergenic | 35.0% | |
| ! | TCTGAGGTAACCAATTTATG+AGG | - | chr8.3:16288970-16288989 | None:intergenic | 35.0% |
| CATCATGAGGTAACTGTTGT+TGG | - | chr8.3:16288675-16288694 | None:intergenic | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | - | chr8.3:16288876-16288895 | None:intergenic | 40.0% | |
| CTAGATGATCATTGATCCAC+AGG | + | chr8.3:16289185-16289204 | MS.gene033178:intron | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | + | chr8.3:16288569-16288588 | MS.gene033178:CDS | 40.0% | |
| TAATAACTCCTCCCTCTACA+TGG | + | chr8.3:16288861-16288880 | MS.gene033178:CDS | 40.0% | |
| TGTGAAGTCACATCATCATG+AGG | - | chr8.3:16288688-16288707 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | - | chr8.3:16288811-16288830 | None:intergenic | 40.0% | |
| ACAAGATTACCTCTGGTCGA+GGG | - | chr8.3:16289213-16289232 | None:intergenic | 45.0% | |
| ACCAGAGGTAATCTTGTCAG+GGG | + | chr8.3:16289216-16289235 | MS.gene033178:intron | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | + | chr8.3:16288547-16288566 | MS.gene033178:CDS | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | + | chr8.3:16288997-16289016 | MS.gene033178:CDS | 45.0% | |
| CCTTGTTGGACAGATGATAG+TGG | - | chr8.3:16289000-16289019 | None:intergenic | 45.0% | |
| CTAAGGAGGCTTATGAATCG+AGG | + | chr8.3:16289094-16289113 | MS.gene033178:CDS | 45.0% | |
| CTACTCCAACAGCAATAGTG+TGG | + | chr8.3:16288834-16288853 | MS.gene033178:CDS | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | + | chr8.3:16288870-16288889 | MS.gene033178:CDS | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | - | chr8.3:16288872-16288891 | None:intergenic | 45.0% | |
| GACCAGAGGTAATCTTGTCA+GGG | + | chr8.3:16289215-16289234 | MS.gene033178:intron | 45.0% | |
| GTACATTTCTGCCTGCAGTT+CGG | + | chr8.3:16289384-16289403 | MS.gene033178:intron | 45.0% | |
| TCATTTAGTACGACCACTCG+AGG | - | chr8.3:16289261-16289280 | None:intergenic | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | - | chr8.3:16288836-16288855 | None:intergenic | 45.0% |
| ACCCCTGACAAGATTACCTC+TGG | - | chr8.3:16289220-16289239 | None:intergenic | 50.0% | |
| CCAATTCTAACCTCCTCGAG+TGG | + | chr8.3:16289245-16289264 | MS.gene033178:intron | 50.0% | |
| CCACTCGAGGAGGTTAGAAT+TGG | - | chr8.3:16289248-16289267 | None:intergenic | 50.0% | |
| CGACCAGAGGTAATCTTGTC+AGG | + | chr8.3:16289214-16289233 | MS.gene033178:intron | 50.0% | |
| GACAAGATTACCTCTGGTCG+AGG | - | chr8.3:16289214-16289233 | None:intergenic | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | - | chr8.3:16288562-16288581 | None:intergenic | 50.0% | |
| TCCTGTGCATGGATGTTGTG+AGG | + | chr8.3:16288558-16288577 | MS.gene033178:CDS | 50.0% | |
| TTTAGTACGACCACTCGAGG+AGG | - | chr8.3:16289258-16289277 | None:intergenic | 50.0% | |
| ! | AAAGAGTGCGAGCGCATTGA+AGG | - | chr8.3:16288737-16288756 | None:intergenic | 50.0% |
| !! | GGGCACTGAAGCCACAAATT+AGG | - | chr8.3:16288910-16288929 | None:intergenic | 50.0% |
| CAGCGATTCTTCCGAACTGC+AGG | - | chr8.3:16289398-16289417 | None:intergenic | 55.0% | |
| !! | TGATAGTGGCTGCGACTCTG+AGG | - | chr8.3:16288986-16289005 | None:intergenic | 55.0% |
| CCACAGGTTCCCTCGACCAG+AGG | + | chr8.3:16289201-16289220 | MS.gene033178:intron | 65.0% | |
| CCTCTGGTCGAGGGAACCTG+TGG | - | chr8.3:16289204-16289223 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 16288517 | 16289504 | 16288517 | ID=MS.gene033178 |
| chr8.3 | mRNA | 16288517 | 16289504 | 16288517 | ID=MS.gene033178.t1;Parent=MS.gene033178 |
| chr8.3 | exon | 16288517 | 16289115 | 16288517 | ID=MS.gene033178.t1.exon1;Parent=MS.gene033178.t1 |
| chr8.3 | CDS | 16288517 | 16289115 | 16288517 | ID=cds.MS.gene033178.t1;Parent=MS.gene033178.t1 |
| chr8.3 | exon | 16289402 | 16289504 | 16289402 | ID=MS.gene033178.t1.exon2;Parent=MS.gene033178.t1 |
| chr8.3 | CDS | 16289402 | 16289504 | 16289402 | ID=cds.MS.gene033178.t1;Parent=MS.gene033178.t1 |
| Gene Sequence |
| Protein sequence |
