Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033199.t1 | XP_013457839.1 | 80.5 | 293 | 19 | 1 | 1 | 293 | 1 | 255 | 1.20E-103 | 386.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033199.t1 | Q9FJK3 | 30.9 | 110 | 72 | 3 | 1 | 109 | 1 | 107 | 2.4e-07 | 57.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033199.t1 | A0A072V1C0 | 80.5 | 293 | 19 | 1 | 1 | 293 | 1 | 255 | 8.9e-104 | 386.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene033199.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033199.t1 | MTR_4g107170 | 78.498 | 293 | 25 | 2 | 1 | 293 | 1 | 255 | 3.30e-165 | 459 |
| MS.gene033199.t1 | MTR_4g045977 | 54.209 | 297 | 105 | 5 | 1 | 293 | 1 | 270 | 7.51e-99 | 296 |
| MS.gene033199.t1 | MTR_2g049610 | 58.333 | 156 | 62 | 1 | 1 | 156 | 1 | 153 | 3.29e-59 | 189 |
| MS.gene033199.t1 | MTR_2g049630 | 47.449 | 196 | 96 | 5 | 1 | 191 | 1 | 194 | 1.14e-51 | 174 |
| MS.gene033199.t1 | MTR_2g049530 | 50.000 | 162 | 78 | 3 | 3 | 162 | 5 | 165 | 8.84e-47 | 155 |
| MS.gene033199.t1 | MTR_1g115290 | 61.538 | 117 | 43 | 1 | 38 | 152 | 2 | 118 | 1.07e-43 | 146 |
| MS.gene033199.t1 | MTR_6g018920 | 38.710 | 155 | 92 | 1 | 1 | 152 | 1 | 155 | 1.02e-35 | 131 |
| MS.gene033199.t1 | MTR_1g114730 | 42.581 | 155 | 86 | 1 | 1 | 152 | 1 | 155 | 1.07e-35 | 131 |
| MS.gene033199.t1 | MTR_6g005440 | 39.744 | 156 | 91 | 1 | 1 | 153 | 1 | 156 | 1.70e-34 | 127 |
| MS.gene033199.t1 | MTR_6g005450 | 34.872 | 195 | 118 | 3 | 1 | 186 | 1 | 195 | 5.48e-30 | 116 |
| MS.gene033199.t1 | MTR_3g466980 | 32.353 | 136 | 86 | 4 | 1 | 135 | 1 | 131 | 7.56e-15 | 72.4 |
| MS.gene033199.t1 | MTR_2g016210 | 28.966 | 145 | 92 | 3 | 2 | 138 | 3 | 144 | 1.14e-13 | 69.7 |
| MS.gene033199.t1 | MTR_3g031240 | 31.776 | 107 | 71 | 1 | 1 | 107 | 1 | 105 | 2.46e-12 | 64.7 |
| MS.gene033199.t1 | MTR_5g075380 | 32.075 | 106 | 68 | 3 | 1 | 105 | 1 | 103 | 2.60e-11 | 61.2 |
| MS.gene033199.t1 | MTR_3g467080 | 31.092 | 119 | 74 | 4 | 1 | 114 | 1 | 116 | 4.27e-11 | 60.8 |
| MS.gene033199.t1 | MTR_4g063790 | 26.115 | 157 | 104 | 4 | 1 | 148 | 1 | 154 | 8.21e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033199.t1 | AT5G55690 | 32.450 | 151 | 91 | 3 | 1 | 147 | 1 | 144 | 8.61e-16 | 76.3 |
| MS.gene033199.t1 | AT5G55690 | 32.450 | 151 | 91 | 3 | 1 | 147 | 1 | 144 | 8.61e-16 | 76.3 |
| MS.gene033199.t1 | AT1G22590 | 35.849 | 106 | 64 | 3 | 1 | 105 | 1 | 103 | 8.05e-11 | 60.1 |
Find 50 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACACTTGTAAGGTCTTC+TGG | 0.270047 | 8.3:+16578368 | None:intergenic |
| TCCTGAGATATTTGATGTTA+AGG | 0.348371 | 8.3:-16578303 | MS.gene033199:CDS |
| GCACTTTGGATGTATTTCAT+AGG | 0.357011 | 8.3:+16578530 | None:intergenic |
| AGCCCACTAAGTTGGTTTGA+TGG | 0.416723 | 8.3:+16578008 | None:intergenic |
| ACCATCTCACTTGTATAATC+AGG | 0.418843 | 8.3:+16577957 | None:intergenic |
| ACAATCTCACTCATATAATC+AGG | 0.433010 | 8.3:+16577846 | None:intergenic |
| GCAAGGAGTTTACACTTGTA+AGG | 0.440354 | 8.3:+16578360 | None:intergenic |
| AGTTGGTTTGATGGTGAACT+AGG | 0.459766 | 8.3:+16578017 | None:intergenic |
| AGTTGGTCTGCTGATGAACT+TGG | 0.474993 | 8.3:+16577795 | None:intergenic |
| GATCAACAAGTTGACTAAGT+TGG | 0.479158 | 8.3:+16577778 | None:intergenic |
| CTAAAATGTGGATGCCAAGT+TGG | 0.479466 | 8.3:+16578203 | None:intergenic |
| TAAAATGTGGATGCCAAGTT+GGG | 0.491000 | 8.3:+16578204 | None:intergenic |
| GCTTGCTTTATTGTGTATGA+TGG | 0.491871 | 8.3:-16578422 | MS.gene033199:CDS |
| TCATCATCAAACCAACTTAG+TGG | 0.501564 | 8.3:-16577900 | MS.gene033199:CDS |
| TCCTTAACATCAAATATCTC+AGG | 0.501707 | 8.3:+16578302 | None:intergenic |
| TCTCCACAAGCCCACTAAGT+TGG | 0.502348 | 8.3:+16578000 | None:intergenic |
| CTTTGAAAATAAGATAGAAA+AGG | 0.507369 | 8.3:-16578276 | MS.gene033199:CDS |
| TCTCCACAAGCCCACTAAGT+TGG | 0.508925 | 8.3:+16577889 | None:intergenic |
| TTGTAAGAAGTCTGGAGCAA+AGG | 0.512867 | 8.3:-16578444 | MS.gene033199:CDS |
| AACTTGTTGATCTTGATGAC+TGG | 0.515181 | 8.3:-16577767 | MS.gene033199:CDS |
| TCACCATCAAACCAACTTAG+TGG | 0.519319 | 8.3:-16578011 | MS.gene033199:CDS |
| ATTGTGGATTTCACTAGTCT+TGG | 0.527589 | 8.3:-16577828 | MS.gene033199:CDS |
| AGTTGGTTTGATGATGAACT+AGG | 0.530891 | 8.3:+16577906 | None:intergenic |
| TATAATCAGGATTAACAACT+TGG | 0.538229 | 8.3:+16577859 | None:intergenic |
| AAACCAACTTAGTGGGCTTG+TGG | 0.552156 | 8.3:-16577892 | MS.gene033199:CDS |
| AAACCAACTTAGTGGGCTTG+TGG | 0.552156 | 8.3:-16578003 | MS.gene033199:CDS |
| CATCATCAAACCAACTTAGT+GGG | 0.554520 | 8.3:-16577899 | MS.gene033199:CDS |
| AGGAGGCAAATCACCAAGAC+TGG | 0.555082 | 8.3:+16577926 | None:intergenic |
| ACTTTCAAACAGAGAAAGCA+TGG | 0.556747 | 8.3:-16578491 | MS.gene033199:CDS |
| AGGTCTTCTGGCCATGTCGT+AGG | 0.557005 | 8.3:+16578380 | None:intergenic |
| ATGGTGGATTTCACCAGTCT+TGG | 0.561657 | 8.3:-16577939 | MS.gene033199:CDS |
| TGATTATATGAGTGAGATTG+TGG | 0.565961 | 8.3:-16577844 | MS.gene033199:CDS |
| TCATCCAAGTTACTAAAATG+TGG | 0.567439 | 8.3:+16578191 | None:intergenic |
| TCACTAAGATCTACCCAACT+TGG | 0.574367 | 8.3:-16578217 | MS.gene033199:CDS |
| TATAATCAGGATCAACAACT+TGG | 0.576059 | 8.3:+16577970 | None:intergenic |
| CACCATCAAACCAACTTAGT+GGG | 0.576425 | 8.3:-16578010 | MS.gene033199:CDS |
| AGTGGGCTTGTGGAGAGGAA+TGG | 0.577682 | 8.3:-16577993 | MS.gene033199:CDS |
| ATTCTGATACTCTTGAAGCA+AGG | 0.580425 | 8.3:+16578343 | None:intergenic |
| TGGTCTGCTGATGAACTTGG+AGG | 0.582147 | 8.3:+16577798 | None:intergenic |
| AACTTAGTGGGCTTGTGGAG+AGG | 0.584533 | 8.3:-16577998 | MS.gene033199:CDS |
| TGATTATACAAGTGAGATGG+TGG | 0.591946 | 8.3:-16577955 | MS.gene033199:CDS |
| ACAAATAGAAACTAGCACCT+TGG | 0.593239 | 8.3:-16578084 | MS.gene033199:CDS |
| TGGTTTGATGGTGAACTAGG+AGG | 0.598871 | 8.3:+16578020 | None:intergenic |
| AATCATGTCACATATATCCA+AGG | 0.609547 | 8.3:+16578067 | None:intergenic |
| TGGATAAATCAATTAGATGA+TGG | 0.616770 | 8.3:-16577747 | MS.gene033199:CDS |
| AAACAAAGATTAAGAAAGTG+CGG | 0.626571 | 8.3:-16578250 | MS.gene033199:CDS |
| TGGTTTGATGATGAACTAGG+AGG | 0.662339 | 8.3:+16577909 | None:intergenic |
| TCCTGATTATACAAGTGAGA+TGG | 0.678910 | 8.3:-16577958 | MS.gene033199:CDS |
| ATAATATTGAACCTACGACA+TGG | 0.717032 | 8.3:-16578391 | MS.gene033199:CDS |
| AAGTTGGGTAGATCTTAGTG+AGG | 0.717752 | 8.3:+16578219 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTGAAAATAAGATAGAAA+AGG | - | chr8.3:16577961-16577980 | MS.gene033199:CDS | 20.0% |
| ! | AAACAAAGATTAAGAAAGTG+CGG | - | chr8.3:16577987-16578006 | MS.gene033199:CDS | 25.0% |
| ! | TATAATCAGGATTAACAACT+TGG | + | chr8.3:16578381-16578400 | None:intergenic | 25.0% |
| ! | TGGATAAATCAATTAGATGA+TGG | - | chr8.3:16578490-16578509 | MS.gene033199:CDS | 25.0% |
| !!! | ATGATTGATTTTTCTGATCT+TGG | - | chr8.3:16578187-16578206 | MS.gene033199:CDS | 25.0% |
| AATCATGTCACATATATCCA+AGG | + | chr8.3:16578173-16578192 | None:intergenic | 30.0% | |
| ACAATCTCACTCATATAATC+AGG | + | chr8.3:16578394-16578413 | None:intergenic | 30.0% | |
| ATAATATTGAACCTACGACA+TGG | - | chr8.3:16577846-16577865 | MS.gene033199:CDS | 30.0% | |
| TATAATCAGGATCAACAACT+TGG | + | chr8.3:16578270-16578289 | None:intergenic | 30.0% | |
| TCATCCAAGTTACTAAAATG+TGG | + | chr8.3:16578049-16578068 | None:intergenic | 30.0% | |
| TCCTGAGATATTTGATGTTA+AGG | - | chr8.3:16577934-16577953 | MS.gene033199:CDS | 30.0% | |
| TCCTTAACATCAAATATCTC+AGG | + | chr8.3:16577938-16577957 | None:intergenic | 30.0% | |
| TCTTTCCAAAAAAACTCATC+AGG | + | chr8.3:16578538-16578557 | None:intergenic | 30.0% | |
| TGATTATATGAGTGAGATTG+TGG | - | chr8.3:16578393-16578412 | MS.gene033199:CDS | 30.0% | |
| ! | TCTGAATTTTGTAAGAAGTC+TGG | - | chr8.3:16577785-16577804 | MS.gene033199:CDS | 30.0% |
| ACAAATAGAAACTAGCACCT+TGG | - | chr8.3:16578153-16578172 | MS.gene033199:CDS | 35.0% | |
| ACCATCTCACTTGTATAATC+AGG | + | chr8.3:16578283-16578302 | None:intergenic | 35.0% | |
| ACTTTCAAACAGAGAAAGCA+TGG | - | chr8.3:16577746-16577765 | MS.gene033199:CDS | 35.0% | |
| ATTCTGATACTCTTGAAGCA+AGG | + | chr8.3:16577897-16577916 | None:intergenic | 35.0% | |
| CATCATCAAACCAACTTAGT+GGG | - | chr8.3:16578338-16578357 | MS.gene033199:CDS | 35.0% | |
| GCACTTTGGATGTATTTCAT+AGG | + | chr8.3:16577710-16577729 | None:intergenic | 35.0% | |
| TAAAATGTGGATGCCAAGTT+GGG | + | chr8.3:16578036-16578055 | None:intergenic | 35.0% | |
| TCATCATCAAACCAACTTAG+TGG | - | chr8.3:16578337-16578356 | MS.gene033199:CDS | 35.0% | |
| TCCTGATTATACAAGTGAGA+TGG | - | chr8.3:16578279-16578298 | MS.gene033199:CDS | 35.0% | |
| TGATTATACAAGTGAGATGG+TGG | - | chr8.3:16578282-16578301 | MS.gene033199:CDS | 35.0% | |
| TTTACACTTGTAAGGTCTTC+TGG | + | chr8.3:16577872-16577891 | None:intergenic | 35.0% | |
| ! | ATTGTGGATTTCACTAGTCT+TGG | - | chr8.3:16578409-16578428 | MS.gene033199:CDS | 35.0% |
| ! | GATCAACAAGTTGACTAAGT+TGG | + | chr8.3:16578462-16578481 | None:intergenic | 35.0% |
| ! | GCTTGCTTTATTGTGTATGA+TGG | - | chr8.3:16577815-16577834 | MS.gene033199:CDS | 35.0% |
| !! | AACTTGTTGATCTTGATGAC+TGG | - | chr8.3:16578470-16578489 | MS.gene033199:CDS | 35.0% |
| !! | AGTTGGTTTGATGATGAACT+AGG | + | chr8.3:16578334-16578353 | None:intergenic | 35.0% |
| !! | GCATCCACATTTTAGTAACT+TGG | - | chr8.3:16578042-16578061 | MS.gene033199:CDS | 35.0% |
| !!! | GTCAACCTGATGAGTTTTTT+TGG | - | chr8.3:16578530-16578549 | MS.gene033199:CDS | 35.0% |
| AAGTTGGGTAGATCTTAGTG+AGG | + | chr8.3:16578021-16578040 | None:intergenic | 40.0% | |
| CACCATCAAACCAACTTAGT+GGG | - | chr8.3:16578227-16578246 | MS.gene033199:CDS | 40.0% | |
| CTAAAATGTGGATGCCAAGT+TGG | + | chr8.3:16578037-16578056 | None:intergenic | 40.0% | |
| GCAAGGAGTTTACACTTGTA+AGG | + | chr8.3:16577880-16577899 | None:intergenic | 40.0% | |
| TCACCATCAAACCAACTTAG+TGG | - | chr8.3:16578226-16578245 | MS.gene033199:CDS | 40.0% | |
| TCACTAAGATCTACCCAACT+TGG | - | chr8.3:16578020-16578039 | MS.gene033199:CDS | 40.0% | |
| ! | TTGTAAGAAGTCTGGAGCAA+AGG | - | chr8.3:16577793-16577812 | MS.gene033199:CDS | 40.0% |
| ! | TTTTGCGAGATTTCGCACTT+TGG | + | chr8.3:16577724-16577743 | None:intergenic | 40.0% |
| !! | AGATGATGGTGTTTTGTGCT+GGG | - | chr8.3:16578504-16578523 | MS.gene033199:CDS | 40.0% |
| !! | AGTTGGTTTGATGGTGAACT+AGG | + | chr8.3:16578223-16578242 | None:intergenic | 40.0% |
| !! | TAGATGATGGTGTTTTGTGC+TGG | - | chr8.3:16578503-16578522 | MS.gene033199:CDS | 40.0% |
| !! | TGGTTTGATGATGAACTAGG+AGG | + | chr8.3:16578331-16578350 | None:intergenic | 40.0% |
| ! | AGTTGGTCTGCTGATGAACT+TGG | + | chr8.3:16578445-16578464 | None:intergenic | 45.0% |
| !! | AAACCAACTTAGTGGGCTTG+TGG | - | chr8.3:16578234-16578253 | MS.gene033199:CDS | 45.0% |
| !! | AGCCCACTAAGTTGGTTTGA+TGG | + | chr8.3:16578232-16578251 | None:intergenic | 45.0% |
| !! | ATGGTGGATTTCACCAGTCT+TGG | - | chr8.3:16578298-16578317 | MS.gene033199:CDS | 45.0% |
| !! | TGGTTTGATGGTGAACTAGG+AGG | + | chr8.3:16578220-16578239 | None:intergenic | 45.0% |
| AGGAGGCAAATCACCAAGAC+TGG | + | chr8.3:16578314-16578333 | None:intergenic | 50.0% | |
| TCTCCACAAGCCCACTAAGT+TGG | + | chr8.3:16578240-16578259 | None:intergenic | 50.0% | |
| TGGTCTGCTGATGAACTTGG+AGG | + | chr8.3:16578442-16578461 | None:intergenic | 50.0% | |
| !! | AACTTAGTGGGCTTGTGGAG+AGG | - | chr8.3:16578239-16578258 | MS.gene033199:CDS | 50.0% |
| AGGTCTTCTGGCCATGTCGT+AGG | + | chr8.3:16577860-16577879 | None:intergenic | 55.0% | |
| ! | AGTGGGCTTGTGGAGAGGAA+TGG | - | chr8.3:16578244-16578263 | MS.gene033199:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 16577689 | 16578570 | 16577689 | ID=MS.gene033199 |
| chr8.3 | mRNA | 16577689 | 16578570 | 16577689 | ID=MS.gene033199.t1;Parent=MS.gene033199 |
| chr8.3 | exon | 16577689 | 16578570 | 16577689 | ID=MS.gene033199.t1.exon1;Parent=MS.gene033199.t1 |
| chr8.3 | CDS | 16577689 | 16578570 | 16577689 | ID=cds.MS.gene033199.t1;Parent=MS.gene033199.t1 |
| Gene Sequence |
| Protein sequence |
