Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033200.t1 | XP_013457839.1 | 80.9 | 293 | 18 | 1 | 1 | 293 | 1 | 255 | 6.50E-105 | 390.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033200.t1 | Q9FJK3 | 31.8 | 110 | 71 | 3 | 1 | 109 | 1 | 107 | 8.3e-08 | 59.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033200.t1 | A0A072V1C0 | 80.9 | 293 | 18 | 1 | 1 | 293 | 1 | 255 | 4.7e-105 | 390.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene033200.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033200.t1 | MTR_4g107170 | 78.840 | 293 | 24 | 2 | 1 | 293 | 1 | 255 | 1.33e-166 | 462 |
| MS.gene033200.t1 | MTR_4g045977 | 48.387 | 341 | 112 | 6 | 1 | 293 | 1 | 325 | 3.40e-96 | 289 |
| MS.gene033200.t1 | MTR_2g049610 | 58.333 | 156 | 62 | 1 | 1 | 156 | 1 | 153 | 4.30e-60 | 191 |
| MS.gene033200.t1 | MTR_2g049630 | 47.449 | 196 | 96 | 5 | 1 | 191 | 1 | 194 | 1.10e-52 | 176 |
| MS.gene033200.t1 | MTR_2g049530 | 50.000 | 162 | 78 | 3 | 3 | 162 | 5 | 165 | 1.58e-47 | 157 |
| MS.gene033200.t1 | MTR_1g115290 | 60.684 | 117 | 44 | 1 | 38 | 152 | 2 | 118 | 1.37e-43 | 146 |
| MS.gene033200.t1 | MTR_6g018920 | 40.000 | 155 | 90 | 1 | 1 | 152 | 1 | 155 | 1.34e-36 | 134 |
| MS.gene033200.t1 | MTR_1g114730 | 42.581 | 155 | 86 | 1 | 1 | 152 | 1 | 155 | 2.39e-36 | 133 |
| MS.gene033200.t1 | MTR_6g005440 | 38.462 | 156 | 93 | 1 | 1 | 153 | 1 | 156 | 3.64e-34 | 126 |
| MS.gene033200.t1 | MTR_6g005450 | 36.774 | 155 | 95 | 1 | 1 | 152 | 1 | 155 | 2.38e-29 | 114 |
| MS.gene033200.t1 | MTR_3g031240 | 34.579 | 107 | 68 | 1 | 1 | 107 | 1 | 105 | 2.06e-14 | 70.5 |
| MS.gene033200.t1 | MTR_3g466980 | 31.618 | 136 | 87 | 4 | 1 | 135 | 1 | 131 | 2.44e-14 | 71.2 |
| MS.gene033200.t1 | MTR_2g016210 | 29.655 | 145 | 91 | 3 | 2 | 138 | 3 | 144 | 3.83e-14 | 70.9 |
| MS.gene033200.t1 | MTR_5g075380 | 32.075 | 106 | 68 | 3 | 1 | 105 | 1 | 103 | 2.58e-11 | 61.2 |
| MS.gene033200.t1 | MTR_3g467080 | 31.092 | 119 | 74 | 4 | 1 | 114 | 1 | 116 | 3.68e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033200.t1 | AT5G55690 | 33.113 | 151 | 90 | 3 | 1 | 147 | 1 | 144 | 3.59e-16 | 77.4 |
| MS.gene033200.t1 | AT5G55690 | 33.113 | 151 | 90 | 3 | 1 | 147 | 1 | 144 | 3.59e-16 | 77.4 |
| MS.gene033200.t1 | AT1G22590 | 35.849 | 106 | 64 | 3 | 1 | 105 | 1 | 103 | 4.73e-11 | 60.8 |
Find 51 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACACTTGTAAGGTCTTC+TGG | 0.270047 | 8.3:+16584468 | None:intergenic |
| TCCTGAGATATTTGATGTTA+AGG | 0.348371 | 8.3:-16584403 | MS.gene033200:CDS |
| GTCAACCTGATGAGTTTGTT+TGG | 0.385592 | 8.3:-16583807 | MS.gene033200:CDS |
| AGCCCACTAAGTTGGTTTGA+TGG | 0.416723 | 8.3:+16584108 | None:intergenic |
| ACCATCTCACTTGTATAATC+AGG | 0.418843 | 8.3:+16584057 | None:intergenic |
| ACAATCTCACTCATATAATC+AGG | 0.433010 | 8.3:+16583946 | None:intergenic |
| GCAAGGAGTTTACACTTGTA+AGG | 0.440354 | 8.3:+16584460 | None:intergenic |
| AGTTGGTTTGATGGTGAACT+AGG | 0.459766 | 8.3:+16584117 | None:intergenic |
| ACTTTCAAACAGAGAAAGAA+TGG | 0.462915 | 8.3:-16584591 | MS.gene033200:CDS |
| TCTTTCCAAACAAACTCATC+AGG | 0.470431 | 8.3:+16583802 | None:intergenic |
| AGTTGGTCTGCTGATGAACT+TGG | 0.474993 | 8.3:+16583895 | None:intergenic |
| GATCAACAAGTTGACTAAGT+TGG | 0.479158 | 8.3:+16583878 | None:intergenic |
| CTAAAATGTGGATGCCAAGT+TGG | 0.479466 | 8.3:+16584303 | None:intergenic |
| TAAAATGTGGATGCCAAGTT+GGG | 0.491000 | 8.3:+16584304 | None:intergenic |
| GCTTGCTTTATTGTGTATGA+TGG | 0.491871 | 8.3:-16584522 | MS.gene033200:CDS |
| TCATCATCAAACCAACTTAG+TGG | 0.501564 | 8.3:-16584000 | MS.gene033200:CDS |
| TCCTTAACATCAAATATCTC+AGG | 0.501707 | 8.3:+16584402 | None:intergenic |
| TCTCCACAAGCCCACTAAGT+TGG | 0.502348 | 8.3:+16584100 | None:intergenic |
| CTTTGAAAATAAGATAGAAA+AGG | 0.507369 | 8.3:-16584376 | MS.gene033200:CDS |
| TCTCCACAAGCCCACTAAGT+TGG | 0.508925 | 8.3:+16583989 | None:intergenic |
| TTGTAAGAAGTCTGGAGCAA+AGG | 0.512867 | 8.3:-16584544 | MS.gene033200:CDS |
| AACTTGTTGATCTTGATGAC+TGG | 0.515181 | 8.3:-16583867 | MS.gene033200:CDS |
| AGGTCTTCTGGCCATGTCGT+TGG | 0.516955 | 8.3:+16584480 | None:intergenic |
| TCACCATCAAACCAACTTAG+TGG | 0.519319 | 8.3:-16584111 | MS.gene033200:CDS |
| ATTGTGGATTTCACTAGTCT+TGG | 0.527589 | 8.3:-16583928 | MS.gene033200:CDS |
| AGTTGGTTTGATGATGAACT+AGG | 0.530891 | 8.3:+16584006 | None:intergenic |
| TATAATCAGGATTAACAACT+TGG | 0.538229 | 8.3:+16583959 | None:intergenic |
| AAACCAACTTAGTGGGCTTG+TGG | 0.552156 | 8.3:-16583992 | MS.gene033200:CDS |
| AAACCAACTTAGTGGGCTTG+TGG | 0.552156 | 8.3:-16584103 | MS.gene033200:CDS |
| CATCATCAAACCAACTTAGT+GGG | 0.554520 | 8.3:-16583999 | MS.gene033200:CDS |
| AGGAGGCAAATCACCAAGAC+TGG | 0.555082 | 8.3:+16584026 | None:intergenic |
| ATGGTGGATTTCACCAGTCT+TGG | 0.561657 | 8.3:-16584039 | MS.gene033200:CDS |
| TGATTATATGAGTGAGATTG+TGG | 0.565961 | 8.3:-16583944 | MS.gene033200:CDS |
| TCATCCAAGTTACTAAAATG+TGG | 0.567439 | 8.3:+16584291 | None:intergenic |
| TCACTAAGATCTACCCAACT+TGG | 0.574367 | 8.3:-16584317 | MS.gene033200:CDS |
| TATAATCAGGATCAACAACT+TGG | 0.576059 | 8.3:+16584070 | None:intergenic |
| CACCATCAAACCAACTTAGT+GGG | 0.576425 | 8.3:-16584110 | MS.gene033200:CDS |
| AGTGGGCTTGTGGAGAGGAA+TGG | 0.577682 | 8.3:-16584093 | MS.gene033200:CDS |
| ATTCTGATACTCTTGAAGCA+AGG | 0.580425 | 8.3:+16584443 | None:intergenic |
| TGGTCTGCTGATGAACTTGG+AGG | 0.582147 | 8.3:+16583898 | None:intergenic |
| AACTTAGTGGGCTTGTGGAG+AGG | 0.584533 | 8.3:-16584098 | MS.gene033200:CDS |
| TGATTATACAAGTGAGATGG+TGG | 0.591946 | 8.3:-16584055 | MS.gene033200:CDS |
| ACAAATAGAAACTAGCACCT+TGG | 0.593239 | 8.3:-16584184 | MS.gene033200:CDS |
| TGGTTTGATGGTGAACTAGG+AGG | 0.598871 | 8.3:+16584120 | None:intergenic |
| AAACAAAGATTAACAAAGTG+CGG | 0.604618 | 8.3:-16584350 | MS.gene033200:CDS |
| AATCATCTCACATATATCCA+AGG | 0.609547 | 8.3:+16584167 | None:intergenic |
| TGGATAAATCAATTAGATGA+TGG | 0.616770 | 8.3:-16583847 | MS.gene033200:CDS |
| ATAATGTTGAACCAACGACA+TGG | 0.647240 | 8.3:-16584491 | MS.gene033200:CDS |
| TGGTTTGATGATGAACTAGG+AGG | 0.662339 | 8.3:+16584009 | None:intergenic |
| TCCTGATTATACAAGTGAGA+TGG | 0.678910 | 8.3:-16584058 | MS.gene033200:CDS |
| AAGTTGGGTAGATCTTAGTG+AGG | 0.717752 | 8.3:+16584319 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTGAAAATAAGATAGAAA+AGG | - | chr8.3:16584061-16584080 | MS.gene033200:CDS | 20.0% |
| ! | AAACAAAGATTAACAAAGTG+CGG | - | chr8.3:16584087-16584106 | MS.gene033200:CDS | 25.0% |
| ! | TATAATCAGGATTAACAACT+TGG | + | chr8.3:16584481-16584500 | None:intergenic | 25.0% |
| ! | TGGATAAATCAATTAGATGA+TGG | - | chr8.3:16584590-16584609 | MS.gene033200:CDS | 25.0% |
| !!! | ATGATTGATTTTTCTGATCT+TGG | - | chr8.3:16584287-16584306 | MS.gene033200:CDS | 25.0% |
| AATCATCTCACATATATCCA+AGG | + | chr8.3:16584273-16584292 | None:intergenic | 30.0% | |
| ACAATCTCACTCATATAATC+AGG | + | chr8.3:16584494-16584513 | None:intergenic | 30.0% | |
| ACTTTCAAACAGAGAAAGAA+TGG | - | chr8.3:16583846-16583865 | MS.gene033200:CDS | 30.0% | |
| TATAATCAGGATCAACAACT+TGG | + | chr8.3:16584370-16584389 | None:intergenic | 30.0% | |
| TCATCCAAGTTACTAAAATG+TGG | + | chr8.3:16584149-16584168 | None:intergenic | 30.0% | |
| TCCTGAGATATTTGATGTTA+AGG | - | chr8.3:16584034-16584053 | MS.gene033200:CDS | 30.0% | |
| TCCTTAACATCAAATATCTC+AGG | + | chr8.3:16584038-16584057 | None:intergenic | 30.0% | |
| TGATTATATGAGTGAGATTG+TGG | - | chr8.3:16584493-16584512 | MS.gene033200:CDS | 30.0% | |
| ! | GCATTTTGGATGTATTTCAT+AGG | + | chr8.3:16583810-16583829 | None:intergenic | 30.0% |
| ! | TCTGAATTTTGTAAGAAGTC+TGG | - | chr8.3:16583885-16583904 | MS.gene033200:CDS | 30.0% |
| ! | TTTTCAACATGCTAACTTTG+TGG | + | chr8.3:16584226-16584245 | None:intergenic | 30.0% |
| ACAAATAGAAACTAGCACCT+TGG | - | chr8.3:16584253-16584272 | MS.gene033200:CDS | 35.0% | |
| ACCATCTCACTTGTATAATC+AGG | + | chr8.3:16584383-16584402 | None:intergenic | 35.0% | |
| ATAATGTTGAACCAACGACA+TGG | - | chr8.3:16583946-16583965 | MS.gene033200:CDS | 35.0% | |
| ATTCTGATACTCTTGAAGCA+AGG | + | chr8.3:16583997-16584016 | None:intergenic | 35.0% | |
| CATCATCAAACCAACTTAGT+GGG | - | chr8.3:16584438-16584457 | MS.gene033200:CDS | 35.0% | |
| TAAAATGTGGATGCCAAGTT+GGG | + | chr8.3:16584136-16584155 | None:intergenic | 35.0% | |
| TCATCATCAAACCAACTTAG+TGG | - | chr8.3:16584437-16584456 | MS.gene033200:CDS | 35.0% | |
| TCCTGATTATACAAGTGAGA+TGG | - | chr8.3:16584379-16584398 | MS.gene033200:CDS | 35.0% | |
| TCTTTCCAAACAAACTCATC+AGG | + | chr8.3:16584638-16584657 | None:intergenic | 35.0% | |
| TGATTATACAAGTGAGATGG+TGG | - | chr8.3:16584382-16584401 | MS.gene033200:CDS | 35.0% | |
| TTTACACTTGTAAGGTCTTC+TGG | + | chr8.3:16583972-16583991 | None:intergenic | 35.0% | |
| ! | ATTGTGGATTTCACTAGTCT+TGG | - | chr8.3:16584509-16584528 | MS.gene033200:CDS | 35.0% |
| ! | GATCAACAAGTTGACTAAGT+TGG | + | chr8.3:16584562-16584581 | None:intergenic | 35.0% |
| ! | GCTTGCTTTATTGTGTATGA+TGG | - | chr8.3:16583915-16583934 | MS.gene033200:CDS | 35.0% |
| ! | GTTTGCGAGATTTTGCATTT+TGG | + | chr8.3:16583824-16583843 | None:intergenic | 35.0% |
| !! | AACTTGTTGATCTTGATGAC+TGG | - | chr8.3:16584570-16584589 | MS.gene033200:CDS | 35.0% |
| !! | AGTTGGTTTGATGATGAACT+AGG | + | chr8.3:16584434-16584453 | None:intergenic | 35.0% |
| !! | GCATCCACATTTTAGTAACT+TGG | - | chr8.3:16584142-16584161 | MS.gene033200:CDS | 35.0% |
| AAGTTGGGTAGATCTTAGTG+AGG | + | chr8.3:16584121-16584140 | None:intergenic | 40.0% | |
| CACCATCAAACCAACTTAGT+GGG | - | chr8.3:16584327-16584346 | MS.gene033200:CDS | 40.0% | |
| CTAAAATGTGGATGCCAAGT+TGG | + | chr8.3:16584137-16584156 | None:intergenic | 40.0% | |
| GCAAGGAGTTTACACTTGTA+AGG | + | chr8.3:16583980-16583999 | None:intergenic | 40.0% | |
| TCACCATCAAACCAACTTAG+TGG | - | chr8.3:16584326-16584345 | MS.gene033200:CDS | 40.0% | |
| TCACTAAGATCTACCCAACT+TGG | - | chr8.3:16584120-16584139 | MS.gene033200:CDS | 40.0% | |
| ! | GTCAACCTGATGAGTTTGTT+TGG | - | chr8.3:16584630-16584649 | MS.gene033200:CDS | 40.0% |
| ! | TTGTAAGAAGTCTGGAGCAA+AGG | - | chr8.3:16583893-16583912 | MS.gene033200:CDS | 40.0% |
| !! | AGATGATGGTGTTTTGTGCT+GGG | - | chr8.3:16584604-16584623 | MS.gene033200:CDS | 40.0% |
| !! | AGTTGGTTTGATGGTGAACT+AGG | + | chr8.3:16584323-16584342 | None:intergenic | 40.0% |
| !! | TAGATGATGGTGTTTTGTGC+TGG | - | chr8.3:16584603-16584622 | MS.gene033200:CDS | 40.0% |
| !! | TGGTTTGATGATGAACTAGG+AGG | + | chr8.3:16584431-16584450 | None:intergenic | 40.0% |
| ! | AGTTGGTCTGCTGATGAACT+TGG | + | chr8.3:16584545-16584564 | None:intergenic | 45.0% |
| !! | AAACCAACTTAGTGGGCTTG+TGG | - | chr8.3:16584334-16584353 | MS.gene033200:CDS | 45.0% |
| !! | AGCCCACTAAGTTGGTTTGA+TGG | + | chr8.3:16584332-16584351 | None:intergenic | 45.0% |
| !! | ATGGTGGATTTCACCAGTCT+TGG | - | chr8.3:16584398-16584417 | MS.gene033200:CDS | 45.0% |
| !! | TGGTTTGATGGTGAACTAGG+AGG | + | chr8.3:16584320-16584339 | None:intergenic | 45.0% |
| AGGAGGCAAATCACCAAGAC+TGG | + | chr8.3:16584414-16584433 | None:intergenic | 50.0% | |
| TCTCCACAAGCCCACTAAGT+TGG | + | chr8.3:16584340-16584359 | None:intergenic | 50.0% | |
| TGGTCTGCTGATGAACTTGG+AGG | + | chr8.3:16584542-16584561 | None:intergenic | 50.0% | |
| !! | AACTTAGTGGGCTTGTGGAG+AGG | - | chr8.3:16584339-16584358 | MS.gene033200:CDS | 50.0% |
| AGGTCTTCTGGCCATGTCGT+TGG | + | chr8.3:16583960-16583979 | None:intergenic | 55.0% | |
| ! | AGTGGGCTTGTGGAGAGGAA+TGG | - | chr8.3:16584344-16584363 | MS.gene033200:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 16583789 | 16584670 | 16583789 | ID=MS.gene033200 |
| chr8.3 | mRNA | 16583789 | 16584670 | 16583789 | ID=MS.gene033200.t1;Parent=MS.gene033200 |
| chr8.3 | exon | 16583789 | 16584670 | 16583789 | ID=MS.gene033200.t1.exon1;Parent=MS.gene033200.t1 |
| chr8.3 | CDS | 16583789 | 16584670 | 16583789 | ID=cds.MS.gene033200.t1;Parent=MS.gene033200.t1 |
| Gene Sequence |
| Protein sequence |
