AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033205

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033205.t1 MTR_4g106540 98.324 358 5 1 1 357 1 358 0.0 727
MS.gene033205.t1 MTR_4g106540 98.039 357 6 1 1 356 1 357 0.0 724
MS.gene033205.t1 MTR_4g106540 96.429 364 6 2 1 357 1 364 0.0 718
MS.gene033205.t1 MTR_4g106540 97.935 339 6 1 1 338 1 339 0.0 680
MS.gene033205.t1 MTR_4g106540 95.942 345 7 2 1 338 1 345 0.0 671
MS.gene033205.t1 MTR_3g113800 46.377 69 37 0 14 82 99 167 1.08e-11 65.5
MS.gene033205.t1 MTR_3g113800 46.377 69 37 0 14 82 99 167 1.33e-11 65.5
MS.gene033205.t1 MTR_4g052000 44.776 67 37 0 17 83 138 204 1.44e-11 65.9
MS.gene033205.t1 MTR_1g105110 44.776 67 37 0 17 83 142 208 3.49e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033205.t1 AT3G48160 55.000 360 130 12 12 356 29 371 2.09e-112 332
MS.gene033205.t1 AT3G48160 54.469 358 133 11 12 355 29 370 1.98e-110 328
MS.gene033205.t1 AT3G01330 47.813 343 150 5 13 355 17 330 1.34e-95 288
MS.gene033205.t1 AT5G14960 41.761 352 169 9 13 354 9 334 7.84e-80 248
MS.gene033205.t1 AT5G14960 37.915 211 114 6 154 354 38 241 2.80e-34 127
MS.gene033205.t1 AT5G22220 44.776 67 37 0 17 83 129 195 4.63e-11 64.3
MS.gene033205.t1 AT5G22220 44.776 67 37 0 17 83 129 195 4.95e-11 64.3

Find 84 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTGGGAAAAGTTGAATT+AGG 0.171110 8.3:-16680551 None:intergenic
AAAGACGATGTTCGTTTAAT+TGG 0.211016 8.3:+16679812 MS.gene033205:CDS
CACCCTGACTTCCAGTGGTT+TGG 0.211662 8.3:-16680484 None:intergenic
GGCAATTCCTGTTGCTTTAC+AGG 0.216414 8.3:+16680164 MS.gene033205:CDS
TTCAGGTGGCTAGGCTTAAA+AGG 0.224076 8.3:+16682123 MS.gene033205:CDS
GACGATGCTGCCGCCAAATT+AGG 0.253633 8.3:+16679839 MS.gene033205:CDS
GTAATAATGAATACCTAATT+TGG 0.256592 8.3:-16679852 None:intergenic
ATTCAAAACAAAACGATTCT+AGG 0.280464 8.3:+16682175 MS.gene033205:CDS
TTTCCAGGTATACTGATTCT+TGG 0.296038 8.3:-16680133 None:intergenic
ACCCTGACTTCCAGTGGTTT+GGG 0.311184 8.3:-16680483 None:intergenic
ATCTGGGAAAAGTTGAATTA+GGG 0.323897 8.3:-16680550 None:intergenic
AATTGCCGCAAAACCTTTCC+AGG 0.340573 8.3:-16680148 None:intergenic
TACCTTCTCGATAAGGTTCA+TGG 0.347489 8.3:-16681934 None:intergenic
TGTTGCTTTACAGGAGCTTA+AGG 0.369256 8.3:+16680173 MS.gene033205:CDS
ATTCTCAGAGCTTCCAGCTT+TGG 0.369624 8.3:-16682391 None:intergenic
ATCCCAAACCACTGGAAGTC+AGG 0.370120 8.3:+16680481 MS.gene033205:CDS
ATTCTGACTTCCAGTGGGTT+GGG 0.370825 8.3:-16680516 None:intergenic
ACTTCAGCTAACTATCAATT+TGG 0.373900 8.3:+16682345 MS.gene033205:CDS
CATTCTGACTTCCAGTGGGT+TGG 0.380743 8.3:-16680517 None:intergenic
GAACAAGTCTGTTCTATTCC+TGG 0.392480 8.3:-16682238 None:intergenic
ATGAAAATGCTTAATTGCCT+TGG 0.399936 8.3:-16682476 None:intergenic
TCAATGTTCTCGAAAGCATC+GGG 0.403367 8.3:+16679984 MS.gene033205:CDS
AAGTGAAGCAGGTGCATGAC+TGG 0.407996 8.3:+16682415 MS.gene033205:CDS
AGTATATTACCTTCTCGATA+AGG 0.412345 8.3:-16681941 None:intergenic
AACAAAACGATTCTAGGAAA+AGG 0.426966 8.3:+16682181 MS.gene033205:CDS
GCACATTTGCAATATCATAA+AGG 0.434271 8.3:-16681905 None:intergenic
GATTCTAGGAAAAGGGCGTT+TGG 0.443366 8.3:+16682189 MS.gene033205:CDS
TTAAGCTCCTGTAAAGCAAC+AGG 0.443519 8.3:-16680171 None:intergenic
ATACAAGAAAACCAGCATTC+AGG 0.443643 8.3:+16682106 MS.gene033205:CDS
AAATGATAGTGGGCTATGTC+TGG 0.446224 8.3:+16682296 MS.gene033205:CDS
CTGGCCTTGCAGCAAAAGTT+AGG 0.448266 8.3:+16681880 MS.gene033205:intron
AAGACAATGGAAAATGATAG+TGG 0.463413 8.3:+16682285 MS.gene033205:CDS
AAGCCTAGCCACCTGAATGC+TGG 0.489317 8.3:-16682117 None:intergenic
CTCTGAGAATAAAGTGAAGC+AGG 0.496045 8.3:+16682404 MS.gene033205:CDS
ACAAAACGATTCTAGGAAAA+GGG 0.498407 8.3:+16682182 MS.gene033205:CDS
ACACTTACTTGGTGCATAAG+AGG 0.500577 8.3:-16679688 None:intergenic
GAATAGAACAGACTTGTTCA+TGG 0.511403 8.3:+16682242 MS.gene033205:CDS
CCTGCCTTTGTATCCAAAGC+TGG 0.512374 8.3:+16682378 MS.gene033205:CDS
ACAACCTTGCTGCATCATCA+AGG 0.524403 8.3:-16681737 None:intergenic
AAGCCAAGAATCAGTATACC+TGG 0.526713 8.3:+16680130 MS.gene033205:CDS
ACTTGGTGCATAAGAGGCCG+AGG 0.537885 8.3:-16679682 None:intergenic
AATCTCAATTCTTCACAAGA+AGG 0.539424 8.3:+16680319 MS.gene033205:CDS
GTCAATGTTCTCGAAAGCAT+CGG 0.541833 8.3:+16679983 MS.gene033205:CDS
TCACAAACATAAGCTTTGCC+AGG 0.545422 8.3:+16682220 MS.gene033205:CDS
AAGAATCAGTATACCTGGAA+AGG 0.546799 8.3:+16680135 MS.gene033205:CDS
GATGCAGCAAGGTTGTTGCT+TGG 0.550005 8.3:+16681744 MS.gene033205:CDS
CCAGCTTTGGATACAAAGGC+AGG 0.550221 8.3:-16682378 None:intergenic
ATCGGAGGAAGAAGCAGCCA+TGG 0.550392 8.3:-16679618 None:intergenic
ATTGTCATTCTGACTTCCAG+TGG 0.553060 8.3:-16680522 None:intergenic
AAACCAGCATTCAGGTGGCT+AGG 0.559723 8.3:+16682114 MS.gene033205:CDS
TCGTAAGTGTGGTGTCGTGA+AGG 0.561541 8.3:-16679644 None:intergenic
TTGTCATTCTGACTTCCAGT+GGG 0.561775 8.3:-16680521 None:intergenic
TCAGGTGGCTAGGCTTAAAA+GGG 0.561931 8.3:+16682124 MS.gene033205:CDS
CTCCATGAACCTTATCGAGA+AGG 0.564896 8.3:+16681932 MS.gene033205:CDS
GATACAAAGGCAGGAGCGAA+AGG 0.576217 8.3:-16682369 None:intergenic
GTTCAGGTGTTGAGAGAAGA+CGG 0.579277 8.3:+16679948 MS.gene033205:intron
TCCCAAACCACTGGAAGTCA+GGG 0.581818 8.3:+16680482 MS.gene033205:CDS
ATAATGAATACCTAATTTGG+CGG 0.587773 8.3:-16679849 None:intergenic
GCTTCCAGCTTTGGATACAA+AGG 0.589316 8.3:-16682382 None:intergenic
GGTATCGGATGAAGAGGATG+AGG 0.589877 8.3:+16680448 MS.gene033205:intron
TGATTCACCCTGACTTCCAG+TGG 0.592103 8.3:-16680489 None:intergenic
AAAAGGGAAGACATTGAATG+AGG 0.592429 8.3:+16682140 MS.gene033205:CDS
AGTGAAGCAGGTGCATGACT+GGG 0.592595 8.3:+16682416 MS.gene033205:CDS
GATTTAGGTATCGGATGAAG+AGG 0.593110 8.3:+16680442 MS.gene033205:intron
TTTGTTTACGATCGTAAGTG+TGG 0.596230 8.3:-16679655 None:intergenic
AATAGAACAGACTTGTTCAT+GGG 0.597678 8.3:+16682243 MS.gene033205:CDS
GTGGTGTCGTGAAGGAGGAT+CGG 0.598361 8.3:-16679636 None:intergenic
CAACCTTGCTGCATCATCAA+GGG 0.604844 8.3:-16681736 None:intergenic
CTCAATTCTTCACAAGAAGG+TGG 0.607030 8.3:+16680322 MS.gene033205:CDS
CTTGGTGCATAAGAGGCCGA+GGG 0.608163 8.3:-16679681 None:intergenic
AGACAATGGAAAATGATAGT+GGG 0.608266 8.3:+16682286 MS.gene033205:CDS
GAATCACTATCCCAACCCAC+TGG 0.620440 8.3:+16680506 MS.gene033205:CDS
GAATTGTTATCCCAAACCAC+TGG 0.620803 8.3:+16680473 MS.gene033205:CDS
TAAGTGTGGTGTCGTGAAGG+AGG 0.625428 8.3:-16679641 None:intergenic
CTTTAAGAAGCAAAAGACAA+TGG 0.627305 8.3:+16682272 MS.gene033205:CDS
AGGTGGCAATGAAGATGTGA+AGG 0.635108 8.3:+16680339 MS.gene033205:CDS
CAAGAAAACCAGCATTCAGG+TGG 0.647844 8.3:+16682109 MS.gene033205:CDS
TGCCCTCAGTATCAAAACCA+AGG 0.661299 8.3:+16682459 MS.gene033205:CDS
GTGTCGTGAAGGAGGATCGG+AGG 0.668512 8.3:-16679633 None:intergenic
CTTCTCGATAAGGTTCATGG+AGG 0.669999 8.3:-16681931 None:intergenic
AGAACAGACTTGTTCATGGG+TGG 0.672206 8.3:+16682246 MS.gene033205:CDS
TGTCTGGAAGATGTGAAACA+AGG 0.683639 8.3:+16682312 MS.gene033205:CDS
CAATGTTCTCGAAAGCATCG+GGG 0.717872 8.3:+16679985 MS.gene033205:CDS
TATCCCTTGATGATGCAGCA+AGG 0.728015 8.3:+16681733 MS.gene033205:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAAATTAATAAAAAAAAG+AGG - chr8.3:16680062-16680081 None:intergenic 10.0%
!! TTATAAAATTCAATAAACAA+TGG - chr8.3:16681448-16681467 None:intergenic 10.0%
!!! GATATTTTTAAATCATTAAA+AGG - chr8.3:16680787-16680806 None:intergenic 10.0%
!!! TTTATTGAATTTTATAATAC+TGG + chr8.3:16681451-16681470 MS.gene033205:intron 10.0%
!!! TTTTATTAATTTTTGTTCAT+CGG + chr8.3:16680067-16680086 MS.gene033205:intron 10.0%
!! AAATTAATAAAAAAAAGAGG+TGG - chr8.3:16680059-16680078 None:intergenic 15.0%
!! AATTAATAAAAAAAAGAGGT+GGG - chr8.3:16680058-16680077 None:intergenic 15.0%
!!! AAATGGTAACTTTTTTATTT+TGG + chr8.3:16680580-16680599 MS.gene033205:intron 15.0%
!!! AATATAAAATGAAAAGTGTA+AGG - chr8.3:16681967-16681986 None:intergenic 15.0%
!!! AATGGTAACTTTTTTATTTT+GGG + chr8.3:16680581-16680600 MS.gene033205:intron 15.0%
!!! ATTTTTATGAGAAGTATAAT+TGG + chr8.3:16680389-16680408 MS.gene033205:intron 15.0%
!!! TAATTGTTTTGATTTTGAAT+GGG + chr8.3:16680271-16680290 MS.gene033205:intron 15.0%
!!! TATTTTGTTTGGTTTTTTTT+AGG + chr8.3:16680095-16680114 MS.gene033205:intron 15.0%
!!! TCACTAATTATTTATTGATT+CGG + chr8.3:16679756-16679775 MS.gene033205:intron 15.0%
!!! TTAAGGTAATTTCAAATTTT+AGG + chr8.3:16680190-16680209 MS.gene033205:intron 15.0%
!!! TTTGTTTTTCTTCAAATTTT+GGG + chr8.3:16680749-16680768 MS.gene033205:intron 15.0%
!!! TTTTGTTTTTCTTCAAATTT+TGG + chr8.3:16680748-16680767 MS.gene033205:intron 15.0%
!! AGAAGAATAAACTGTAAATA+TGG - chr8.3:16680703-16680722 None:intergenic 20.0%
!! GTAATAATGAATACCTAATT+TGG - chr8.3:16679855-16679874 None:intergenic 20.0%
!! TATTCTTCTAAATGCATTAT+AGG + chr8.3:16680714-16680733 MS.gene033205:intron 20.0%
!! TCACAGAATTTAAATTAAGA+AGG - chr8.3:16680224-16680243 None:intergenic 20.0%
!! TTAACTAAATTATCATTCTC+TGG - chr8.3:16681410-16681429 None:intergenic 20.0%
!!! GTAATTGTTTTGATTTTGAA+TGG + chr8.3:16680270-16680289 MS.gene033205:intron 20.0%
! AATTTAGTTAAAGATGTGCA+CGG + chr8.3:16681419-16681438 MS.gene033205:intron 25.0%
! AGTTTAACATATCAGATGTA+TGG - chr8.3:16681671-16681690 None:intergenic 25.0%
! ATAAAGAAATCAATGCAAAG+AGG - chr8.3:16682043-16682062 None:intergenic 25.0%
! ATAATGAATACCTAATTTGG+CGG - chr8.3:16679852-16679871 None:intergenic 25.0%
! ATTAAAGACATAAATCAGCT+TGG - chr8.3:16681641-16681660 None:intergenic 25.0%
! ATTCAAAACAAAACGATTCT+AGG + chr8.3:16682175-16682194 MS.gene033205:CDS 25.0%
! GTTAACGAAAACAAACAAAT+TGG - chr8.3:16681821-16681840 None:intergenic 25.0%
! TGCAATTTGCAAATTAAAGT+TGG - chr8.3:16680938-16680957 None:intergenic 25.0%
! TTTAAGTACTTTCGTGATTT+AGG + chr8.3:16680427-16680446 MS.gene033205:intron 25.0%
!! AACCCTTATTTAGTTAACTT+GGG - chr8.3:16681027-16681046 None:intergenic 25.0%
!! AGAAGTATAATTGGTTTAGT+AGG + chr8.3:16680398-16680417 MS.gene033205:intron 25.0%
!! GAAGTATAATTGGTTTAGTA+GGG + chr8.3:16680399-16680418 MS.gene033205:intron 25.0%
!! TAGGATGTTTTCTTTTCTTA+GGG + chr8.3:16681305-16681324 MS.gene033205:intron 25.0%
!! TTAGGATGTTTTCTTTTCTT+AGG + chr8.3:16681304-16681323 MS.gene033205:intron 25.0%
!!! ATCAGATTGTTCAAGTTTTT+AGG + chr8.3:16681286-16681305 MS.gene033205:intron 25.0%
!!! CATCGGTTTTTTATTTTGTT+TGG + chr8.3:16680084-16680103 MS.gene033205:intron 25.0%
!!! TAGCAGAATTTTTGAAACAT+GGG + chr8.3:16680880-16680899 MS.gene033205:intron 25.0%
!!! TTACCATTTTTCAAAGATCT+GGG - chr8.3:16680569-16680588 None:intergenic 25.0%
!!! TTGATTTTGAATGGGTTTTT+AGG + chr8.3:16680279-16680298 MS.gene033205:intron 25.0%
!!! TTGTTTTGTTCGAATGTAAA+TGG - chr8.3:16681340-16681359 None:intergenic 25.0%
AAAGACGATGTTCGTTTAAT+TGG + chr8.3:16679812-16679831 MS.gene033205:CDS 30.0%
AACAGTTGTTAATGATGTTC+AGG + chr8.3:16679932-16679951 MS.gene033205:intron 30.0%
AACCCCAAGTTAACTAAATA+AGG + chr8.3:16681021-16681040 MS.gene033205:intron 30.0%
AAGACAATGGAAAATGATAG+TGG + chr8.3:16682285-16682304 MS.gene033205:CDS 30.0%
AATCTCAATTCTTCACAAGA+AGG + chr8.3:16680319-16680338 MS.gene033205:CDS 30.0%
ACATGAGGGAACAAAAATTA+AGG - chr8.3:16680034-16680053 None:intergenic 30.0%
ACCCCAAGTTAACTAAATAA+GGG + chr8.3:16681022-16681041 MS.gene033205:intron 30.0%
ACTTCAGCTAACTATCAATT+TGG + chr8.3:16682345-16682364 MS.gene033205:CDS 30.0%
AGACAATGGAAAATGATAGT+GGG + chr8.3:16682286-16682305 MS.gene033205:CDS 30.0%
AGTATATTACCTTCTCGATA+AGG - chr8.3:16681944-16681963 None:intergenic 30.0%
ATCTGGGAAAAGTTGAATTA+GGG - chr8.3:16680553-16680572 None:intergenic 30.0%
CAAAAGACATAATACAGGTA+TGG - chr8.3:16681208-16681227 None:intergenic 30.0%
CCAAGTGTTTGTTTAGATTA+AGG - chr8.3:16681155-16681174 None:intergenic 30.0%
CCTTAATCTAAACAAACACT+TGG + chr8.3:16681152-16681171 MS.gene033205:intron 30.0%
GCACATTTGCAATATCATAA+AGG - chr8.3:16681908-16681927 None:intergenic 30.0%
TAAGAAAAACTAGAGTAGAG+TGG + chr8.3:16680825-16680844 MS.gene033205:intron 30.0%
TACTTTCGTGATTTAGGTAT+CGG + chr8.3:16680433-16680452 MS.gene033205:intron 30.0%
TGAACAATCTGATGAGAATT+GGG - chr8.3:16681279-16681298 None:intergenic 30.0%
TTGAACAATCTGATGAGAAT+TGG - chr8.3:16681280-16681299 None:intergenic 30.0%
! AACAAAACGATTCTAGGAAA+AGG + chr8.3:16682181-16682200 MS.gene033205:CDS 30.0%
! AATAGAACAGACTTGTTCAT+GGG + chr8.3:16682243-16682262 MS.gene033205:CDS 30.0%
! ACAAAACGATTCTAGGAAAA+GGG + chr8.3:16682182-16682201 MS.gene033205:CDS 30.0%
! ACCCTTATTTAGTTAACTTG+GGG - chr8.3:16681026-16681045 None:intergenic 30.0%
! CTTTAAGAAGCAAAAGACAA+TGG + chr8.3:16682272-16682291 MS.gene033205:CDS 30.0%
! GAACCCTTATTTAGTTAACT+TGG - chr8.3:16681028-16681047 None:intergenic 30.0%
! GGCGATAATTTGAATTTTTG+AGG + chr8.3:16680846-16680865 MS.gene033205:intron 30.0%
! TGTTTTCTTTTCTTAGGGTT+AGG + chr8.3:16681310-16681329 MS.gene033205:intron 30.0%
! TTAATGTTTTGCAGACAGAA+GGG + chr8.3:16681531-16681550 MS.gene033205:intron 30.0%
! TTTAATGTTTTGCAGACAGA+AGG + chr8.3:16681530-16681549 MS.gene033205:intron 30.0%
! TTTCCCAGATCTTTGAAAAA+TGG + chr8.3:16680563-16680582 MS.gene033205:CDS 30.0%
! TTTTCTTGTATCTGTGGTAT+GGG - chr8.3:16682097-16682116 None:intergenic 30.0%
!! GTTACCATTTTTCAAAGATC+TGG - chr8.3:16680570-16680589 None:intergenic 30.0%
!!! GAATTTTTGAAACATGGGTT+TGG + chr8.3:16680885-16680904 MS.gene033205:intron 30.0%
!!! GTAGCAGAATTTTTGAAACA+TGG + chr8.3:16680879-16680898 MS.gene033205:intron 30.0%
AAAAGGGAAGACATTGAATG+AGG + chr8.3:16682140-16682159 MS.gene033205:CDS 35.0%
AACATATCAGATGTATGGCA+TGG - chr8.3:16681666-16681685 None:intergenic 35.0%
AAGAATCAGTATACCTGGAA+AGG + chr8.3:16680135-16680154 MS.gene033205:CDS 35.0%
ACACACACATTAATACGTTG+TGG + chr8.3:16681099-16681118 MS.gene033205:intron 35.0%
ATACAAGAAAACCAGCATTC+AGG + chr8.3:16682106-16682125 MS.gene033205:CDS 35.0%
ATTAATGTGTGTGTAGACTG+AGG - chr8.3:16681093-16681112 None:intergenic 35.0%
CACACACATTAATACGTTGT+GGG + chr8.3:16681100-16681119 MS.gene033205:intron 35.0%
CTTGGAATGAACAAGAAACT+AGG - chr8.3:16681623-16681642 None:intergenic 35.0%
GAGTAAGAGTAACACTTACT+TGG - chr8.3:16679702-16679721 None:intergenic 35.0%
GATCGTAAACAAAAATCCCT+CGG + chr8.3:16679665-16679684 MS.gene033205:CDS 35.0%
GATCTGGGAAAAGTTGAATT+AGG - chr8.3:16680554-16680573 None:intergenic 35.0%
GTGCACAAAAGACATAATAC+AGG - chr8.3:16681213-16681232 None:intergenic 35.0%
TATAATACTGGAGTCGTATG+TGG + chr8.3:16681463-16681482 MS.gene033205:intron 35.0%
TTTGTTTACGATCGTAAGTG+TGG - chr8.3:16679658-16679677 None:intergenic 35.0%
! GAATAGAACAGACTTGTTCA+TGG + chr8.3:16682242-16682261 MS.gene033205:CDS 35.0%
! GTTTTCTTGTATCTGTGGTA+TGG - chr8.3:16682098-16682117 None:intergenic 35.0%
!! CGTTGTGGGTTATAGTTTTA+AGG + chr8.3:16681114-16681133 MS.gene033205:intron 35.0%
!! GTTTTTAGGAAGAGGGTTTA+TGG + chr8.3:16680293-16680312 MS.gene033205:intron 35.0%
!! TGAATGGGTTTTTAGGAAGA+GGG + chr8.3:16680286-16680305 MS.gene033205:intron 35.0%
!! TTATGAACCTGTGTTTTTGC+TGG + chr8.3:16680622-16680641 MS.gene033205:intron 35.0%
!! TTGAATGGGTTTTTAGGAAG+AGG + chr8.3:16680285-16680304 MS.gene033205:intron 35.0%
!! TTTCCAGGTATACTGATTCT+TGG - chr8.3:16680136-16680155 None:intergenic 35.0%
AAAAAAGAGGTGGGAACATG+AGG - chr8.3:16680049-16680068 None:intergenic 40.0%
AAAAAGAGGTGGGAACATGA+GGG - chr8.3:16680048-16680067 None:intergenic 40.0%
AACAACACCAGCAAAAACAC+AGG - chr8.3:16680632-16680651 None:intergenic 40.0%
AAGCCAAGAATCAGTATACC+TGG + chr8.3:16680130-16680149 MS.gene033205:CDS 40.0%
AGACAGAAGGGAAAAATCTC+TGG + chr8.3:16681543-16681562 MS.gene033205:intron 40.0%
ATTGTCATTCTGACTTCCAG+TGG - chr8.3:16680525-16680544 None:intergenic 40.0%
CTATGCTTACCCAGCAAAAT+TGG - chr8.3:16681054-16681073 None:intergenic 40.0%
CTCAATTCTTCACAAGAAGG+TGG + chr8.3:16680322-16680341 MS.gene033205:CDS 40.0%
GAACAAGTCTGTTCTATTCC+TGG - chr8.3:16682241-16682260 None:intergenic 40.0%
GAATTGTTATCCCAAACCAC+TGG + chr8.3:16680473-16680492 MS.gene033205:CDS 40.0%
TACCTTCTCGATAAGGTTCA+TGG - chr8.3:16681937-16681956 None:intergenic 40.0%
TCACAAACATAAGCTTTGCC+AGG + chr8.3:16682220-16682239 MS.gene033205:CDS 40.0%
TGAGAATTGGGATTGTTCTG+AGG - chr8.3:16681267-16681286 None:intergenic 40.0%
TGTCTGGAAGATGTGAAACA+AGG + chr8.3:16682312-16682331 MS.gene033205:CDS 40.0%
TTAAGCTCCTGTAAAGCAAC+AGG - chr8.3:16680174-16680193 None:intergenic 40.0%
TTGTCATTCTGACTTCCAGT+GGG - chr8.3:16680524-16680543 None:intergenic 40.0%
! AGGGTTCAATCCAATTTTGC+TGG + chr8.3:16681041-16681060 MS.gene033205:intron 40.0%
! CAATTTTGCTGGGTAAGCAT+AGG + chr8.3:16681052-16681071 MS.gene033205:intron 40.0%
! CTCTGAGAATAAAGTGAAGC+AGG + chr8.3:16682404-16682423 MS.gene033205:CDS 40.0%
! GGGTTCAATCCAATTTTGCT+GGG + chr8.3:16681042-16681061 MS.gene033205:intron 40.0%
! GTATACCTGGAAAGGTTTTG+CGG + chr8.3:16680143-16680162 MS.gene033205:CDS 40.0%
! GTCAATGTTCTCGAAAGCAT+CGG + chr8.3:16679983-16680002 MS.gene033205:CDS 40.0%
! TTTTCACTTACAGTCCCATC+TGG + chr8.3:16681861-16681880 MS.gene033205:intron 40.0%
!! AAATGATAGTGGGCTATGTC+TGG + chr8.3:16682296-16682315 MS.gene033205:CDS 40.0%
!! ACACTTACTTGGTGCATAAG+AGG - chr8.3:16679691-16679710 None:intergenic 40.0%
!! GATTTAGGTATCGGATGAAG+AGG + chr8.3:16680442-16680461 MS.gene033205:intron 40.0%
!! TCAATGTTCTCGAAAGCATC+GGG + chr8.3:16679984-16680003 MS.gene033205:CDS 40.0%
!! TGTTGCTTTACAGGAGCTTA+AGG + chr8.3:16680173-16680192 MS.gene033205:CDS 40.0%
!!! TGCCTTGGTTTTGATACTGA+GGG - chr8.3:16682464-16682483 None:intergenic 40.0%
!!! TGCTGGTTTTCTTGTATCTG+TGG - chr8.3:16682103-16682122 None:intergenic 40.0%
!!! TTGCCTTGGTTTTGATACTG+AGG - chr8.3:16682465-16682484 None:intergenic 40.0%
!!! TTGGCACTGATTGCATTTTG+AGG + chr8.3:16681494-16681513 MS.gene033205:intron 40.0%
!!! TTTTGCTGGTGTTGTTGTTG+TGG + chr8.3:16680636-16680655 MS.gene033205:intron 40.0%
AATTGCCGCAAAACCTTTCC+AGG - chr8.3:16680151-16680170 None:intergenic 45.0%
ACAACCTTGCTGCATCATCA+AGG - chr8.3:16681740-16681759 None:intergenic 45.0%
AGGTGGCAATGAAGATGTGA+AGG + chr8.3:16680339-16680358 MS.gene033205:CDS 45.0%
ATTCTCAGAGCTTCCAGCTT+TGG - chr8.3:16682394-16682413 None:intergenic 45.0%
CAACCTTGCTGCATCATCAA+GGG - chr8.3:16681739-16681758 None:intergenic 45.0%
CAAGAAAACCAGCATTCAGG+TGG + chr8.3:16682109-16682128 MS.gene033205:CDS 45.0%
CTCCATGAACCTTATCGAGA+AGG + chr8.3:16681932-16681951 MS.gene033205:CDS 45.0%
CTTCTCGATAAGGTTCATGG+AGG - chr8.3:16681934-16681953 None:intergenic 45.0%
GTCGTATGTGGACTTAGACT+TGG + chr8.3:16681475-16681494 MS.gene033205:intron 45.0%
TCAGGTGGCTAGGCTTAAAA+GGG + chr8.3:16682124-16682143 MS.gene033205:CDS 45.0%
TGCCCTCAGTATCAAAACCA+AGG + chr8.3:16682459-16682478 MS.gene033205:CDS 45.0%
TTCAGGTGGCTAGGCTTAAA+AGG + chr8.3:16682123-16682142 MS.gene033205:CDS 45.0%
! AGAACAGACTTGTTCATGGG+TGG + chr8.3:16682246-16682265 MS.gene033205:CDS 45.0%
! ATTCTGACTTCCAGTGGGTT+GGG - chr8.3:16680519-16680538 None:intergenic 45.0%
!! CAATGTTCTCGAAAGCATCG+GGG + chr8.3:16679985-16680004 MS.gene033205:CDS 45.0%
!! GATTCTAGGAAAAGGGCGTT+TGG + chr8.3:16682189-16682208 MS.gene033205:CDS 45.0%
!! GCTTCCAGCTTTGGATACAA+AGG - chr8.3:16682385-16682404 None:intergenic 45.0%
!! GGCAATTCCTGTTGCTTTAC+AGG + chr8.3:16680164-16680183 MS.gene033205:CDS 45.0%
!! GTTCAGGTGTTGAGAGAAGA+CGG + chr8.3:16679948-16679967 MS.gene033205:intron 45.0%
!! TATCCCTTGATGATGCAGCA+AGG + chr8.3:16681733-16681752 MS.gene033205:CDS 45.0%
!! AAAAATAAAATAAAACAAAT+TGG + chr8.3:16679900-16679919 MS.gene033205:intron 5.0%
AAACCAGCATTCAGGTGGCT+AGG + chr8.3:16682114-16682133 MS.gene033205:CDS 50.0%
AAGTGAAGCAGGTGCATGAC+TGG + chr8.3:16682415-16682434 MS.gene033205:CDS 50.0%
ACCCTGACTTCCAGTGGTTT+GGG - chr8.3:16680486-16680505 None:intergenic 50.0%
AGTGAAGCAGGTGCATGACT+GGG + chr8.3:16682416-16682435 MS.gene033205:CDS 50.0%
ATCCCAAACCACTGGAAGTC+AGG + chr8.3:16680481-16680500 MS.gene033205:CDS 50.0%
CATTCTGACTTCCAGTGGGT+TGG - chr8.3:16680520-16680539 None:intergenic 50.0%
CCTGCCTTTGTATCCAAAGC+TGG + chr8.3:16682378-16682397 MS.gene033205:CDS 50.0%
GAATCACTATCCCAACCCAC+TGG + chr8.3:16680506-16680525 MS.gene033205:CDS 50.0%
GATACAAAGGCAGGAGCGAA+AGG - chr8.3:16682372-16682391 None:intergenic 50.0%
GATGCAGCAAGGTTGTTGCT+TGG + chr8.3:16681744-16681763 MS.gene033205:CDS 50.0%
TAACCACTCTACTCTGCAGC+TGG + chr8.3:16681704-16681723 MS.gene033205:intron 50.0%
TCCCAAACCACTGGAAGTCA+GGG + chr8.3:16680482-16680501 MS.gene033205:CDS 50.0%
TGATTCACCCTGACTTCCAG+TGG - chr8.3:16680492-16680511 None:intergenic 50.0%
TTTCCAGCTGCAGAGTAGAG+TGG - chr8.3:16681710-16681729 None:intergenic 50.0%
! ACTTTTGCTGCAAGGCCAGA+TGG - chr8.3:16681879-16681898 None:intergenic 50.0%
! CTTTTGCTGCAAGGCCAGAT+GGG - chr8.3:16681878-16681897 None:intergenic 50.0%
! GGTATCGGATGAAGAGGATG+AGG + chr8.3:16680448-16680467 MS.gene033205:intron 50.0%
!! CCAGCTTTGGATACAAAGGC+AGG - chr8.3:16682381-16682400 None:intergenic 50.0%
!! CTGGCCTTGCAGCAAAAGTT+AGG + chr8.3:16681880-16681899 MS.gene033205:intron 50.0%
!! TAAGTGTGGTGTCGTGAAGG+AGG - chr8.3:16679644-16679663 None:intergenic 50.0%
!! TCGTAAGTGTGGTGTCGTGA+AGG - chr8.3:16679647-16679666 None:intergenic 50.0%
AAGCCTAGCCACCTGAATGC+TGG - chr8.3:16682120-16682139 None:intergenic 55.0%
CACCCTGACTTCCAGTGGTT+TGG - chr8.3:16680487-16680506 None:intergenic 55.0%
CTGGGTAAGCATAGGTCGAG+GGG + chr8.3:16681060-16681079 MS.gene033205:intron 55.0%
GACGATGCTGCCGCCAAATT+AGG + chr8.3:16679839-16679858 MS.gene033205:CDS 55.0%
GCTGGGTAAGCATAGGTCGA+GGG + chr8.3:16681059-16681078 MS.gene033205:intron 55.0%
TGCTGGGTAAGCATAGGTCG+AGG + chr8.3:16681058-16681077 MS.gene033205:intron 55.0%
! CTTGGTGCATAAGAGGCCGA+GGG - chr8.3:16679684-16679703 None:intergenic 55.0%
! GGCGCCTAACTTTTGCTGCA+AGG - chr8.3:16681887-16681906 None:intergenic 55.0%
!! ACTTGGTGCATAAGAGGCCG+AGG - chr8.3:16679685-16679704 None:intergenic 55.0%
!! GTGGTGTCGTGAAGGAGGAT+CGG - chr8.3:16679639-16679658 None:intergenic 55.0%
GTGTCGTGAAGGAGGATCGG+AGG - chr8.3:16679636-16679655 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 16679620 16682488 16679620 ID=MS.gene033205
chr8.3 mRNA 16679620 16682488 16679620 ID=MS.gene033205.t1;Parent=MS.gene033205
chr8.3 exon 16679620 16679702 16679620 ID=MS.gene033205.t1.exon1;Parent=MS.gene033205.t1
chr8.3 CDS 16679620 16679702 16679620 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16679793 16679860 16679793 ID=MS.gene033205.t1.exon2;Parent=MS.gene033205.t1
chr8.3 CDS 16679793 16679860 16679793 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16679954 16680006 16679954 ID=MS.gene033205.t1.exon3;Parent=MS.gene033205.t1
chr8.3 CDS 16679954 16680006 16679954 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16680117 16680194 16680117 ID=MS.gene033205.t1.exon4;Parent=MS.gene033205.t1
chr8.3 CDS 16680117 16680194 16680117 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16680301 16680360 16680301 ID=MS.gene033205.t1.exon5;Parent=MS.gene033205.t1
chr8.3 CDS 16680301 16680360 16680301 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16680449 16680584 16680449 ID=MS.gene033205.t1.exon6;Parent=MS.gene033205.t1
chr8.3 CDS 16680449 16680584 16680449 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16681545 16681609 16681545 ID=MS.gene033205.t1.exon7;Parent=MS.gene033205.t1
chr8.3 CDS 16681545 16681609 16681545 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16681723 16681795 16681723 ID=MS.gene033205.t1.exon8;Parent=MS.gene033205.t1
chr8.3 CDS 16681723 16681795 16681723 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16681892 16681953 16681892 ID=MS.gene033205.t1.exon9;Parent=MS.gene033205.t1
chr8.3 CDS 16681892 16681953 16681892 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
chr8.3 exon 16682093 16682488 16682093 ID=MS.gene033205.t1.exon10;Parent=MS.gene033205.t1
chr8.3 CDS 16682093 16682488 16682093 ID=cds.MS.gene033205.t1;Parent=MS.gene033205.t1
Gene Sequence

>MS.gene033205

ATGGCTGCTTCTTCCTCCGATCCTCCTTCACGACACCACACTTACGATCGTAAACAAAAATCCCTCGGCCTCTTATGCACCAAGTAAGTGTTACTCTTACTCTTCTTCTCTTCTCTCTCTCACTTAATTCTTTTATTCACTAATTATTTATTGATTCGGTTTATGAATTGCAGTTTCTTGAGCTTGTATAACAAAGACGATGTTCGTTTAATTGGTCTCGACGATGCTGCCGCCAAATTAGGTATTCATTATTACGATTCACTTTTTAGTTTTTTAATTTAAAAATAAAATAAAACAAATTGGATCTAATACAACAGTTGTTAATGATGTTCAGGTGTTGAGAGAAGACGGATCTATGATATTGTCAATGTTCTCGAAAGCATCGGGGTTCGTTTATTTTTTATTTTTTAACCTTAATTTTTGTTCCCTCATGTTCCCACCTCTTTTTTTTATTAATTTTTGTTCATCGGTTTTTTATTTTGTTTGGTTTTTTTTAGGTTCTTGCAAGAAAAGCCAAGAATCAGTATACCTGGAAAGGTTTTGCGGCAATTCCTGTTGCTTTACAGGAGCTTAAGGTAATTTCAAATTTTAGGATTCAGTTCCTTCTTAATTTAAATTCTGTGATTCAATAATTGTAATTTATTTTTTATGTAATTGTTTTGATTTTGAATGGGTTTTTAGGAAGAGGGTTTATGGCAGAATCTCAATTCTTCACAAGAAGGTGGCAATGAAGATGTGAAGGTGAGTTCTATTTTCACATACATAATTTATTTTTATGAGAAGTATAATTGGTTTAGTAGGGTTGTTTTTAAGTACTTTCGTGATTTAGGTATCGGATGAAGAGGATGAGGACGAATTGTTATCCCAAACCACTGGAAGTCAGGGTGAATCACTATCCCAACCCACTGGAAGTCAGAATGACAATCTAAACCCTAATTCAACTTTTCCCAGATCTTTGAAAAATGGTAACTTTTTTATTTTGGGTACAAACATGTTTAAGATTTATGAACCTGTGTTTTTGCTGGTGTTGTTGTTGTGGTATATGATGTTTGTTCTGTCTTGTAATGTTTTACTAAAAAACCATATTTACAGTTTATTCTTCTAAATGCATTATAGGTCAGTAGATTATTTTGTTTTTCTTCAAATTTTGGGTCACTGAATTAGCCTTTTAATGATTTAAAAATATCATCATAATTTTGATTTTTTAAGAAAAACTAGAGTAGAGTGGCGATAATTTGAATTTTTGAGGAACTACTTTAGTAGCAGAATTTTTGAAACATGGGTTTGGAACAAACTTTTATAACTTAAGTTTTAGCCAACTTTAATTTGCAAATTGCAAAAACAATTCTCTCTGCATACTATTAATAATTATGTTTGATGACAAAAAATGTGTTTTTAAATAACCCCAAGTTAACTAAATAAGGGTTCAATCCAATTTTGCTGGGTAAGCATAGGTCGAGGGGTCTTTAACCTCAGTCTACACACACATTAATACGTTGTGGGTTATAGTTTTAAGGTGCTATTGTACTCTACCTTAATCTAAACAAACACTTGGTTGTTAGAGTTTTCTTCTACATATTGTATGCCATACCTGTATTATGTCTTTTGTGCACGATTGCGAGAGATTTATTTTTACTTTTGTATCCTCAGAACAATCCCAATTCTCATCAGATTGTTCAAGTTTTTAGGATGTTTTCTTTTCTTAGGGTTAGGAATACCATTTACATTCGAACAAAACAACTTTTAATATCGTATGATACTGTTAGTGATTATGACTGTTATTAGCACCAGAGAATGATAATTTAGTTAAAGATGTGCACGGATACCATTGTTTATTGAATTTTATAATACTGGAGTCGTATGTGGACTTAGACTTGGCACTGATTGCATTTTGAGGCTCACTTTTCTACTTTAATGTTTTGCAGACAGAAGGGAAAAATCTCTGGCGCTGCTTACTCAGAATTTTGTCAAGCTCTTTGTCTGTTCTAACGTGAGAATTGCCTAGTTTCTTGTTCATTCCAAGCTGATTTATGTCTTTAATATCCATGCCATACATCTGATATGTTAAACTTGTTGACTAACGATAACCACTCTACTCTGCAGCTGGAAATGATATCCCTTGATGATGCAGCAAGGTTGTTGCTTGGAGATGCATATAATTCATCAACAATGAGAAGTAAGTAATATAAAATGAATTACCAATTTGTTTGTTTTCGTTAACAAAAGAGTCAGCAACGAATCTTTTCACTTACAGTCCCATCTGGCCTTGCAGCAAAAGTTAGGCGCCTTTATGATATTGCAAATGTGCTAACCTCCATGAACCTTATCGAGAAGGTAATATACTCCTTACACTTTTCATTTTATATTTTTTATTTGTTCAAATTTACTAGTAAATAGTAGTGAGAATGAGTTTCACATTTCCTCTTTGCATTGATTTCTTTATTGATTATTGTCACTCTACAAAATGATGCAGACCCATACCACAGATACAAGAAAACCAGCATTCAGGTGGCTAGGCTTAAAAGGGAAGACATTGAATGAGGCATCACTTTACAATTCAAAACAAAACGATTCTAGGAAAAGGGCGTTTGGAAATGATGTCACAAACATAAGCTTTGCCAGGAATAGAACAGACTTGTTCATGGGTGGTGACTTTAAGAAGCAAAAGACAATGGAAAATGATAGTGGGCTATGTCTGGAAGATGTGAAACAAGGCATAAAACAGACTTCAGCTAACTATCAATTTGGTCCTTTCGCTCCTGCCTTTGTATCCAAAGCTGGAAGCTCTGAGAATAAAGTGAAGCAGGTGCATGACTGGGAGAGTCTCGCTACTGAACATTGCCCTCAGTATCAAAACCAAGGCAATTAA
Protein sequence

>MS.gene033205.t1

MAASSSDPPSRHHTYDRKQKSLGLLCTNFLSLYNKDDVRLIGLDDAAAKLGVERRRIYDIVNVLESIGVLARKAKNQYTWKGFAAIPVALQELKEEGLWQNLNSSQEGGNEDVKVSDEEDEDELLSQTTGSQGESLSQPTGSQNDNLNPNSTFPRSLKNDRREKSLALLTQNFVKLFVCSNLEMISLDDAARLLLGDAYNSSTMRTKVRRLYDIANVLTSMNLIEKTHTTDTRKPAFRWLGLKGKTLNEASLYNSKQNDSRKRAFGNDVTNISFARNRTDLFMGGDFKKQKTMENDSGLCLEDVKQGIKQTSANYQFGPFAPAFVSKAGSSENKVKQVHDWESLATEHCPQYQNQGN