AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033341

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033341.t1 MTR_4g104020 98.851 522 5 1 1 522 1 521 0.0 1075
MS.gene033341.t1 MTR_8g442410 46.214 383 195 5 144 521 149 525 1.37e-104 323
MS.gene033341.t1 MTR_7g027190 41.191 403 207 7 134 522 288 674 6.94e-95 303
MS.gene033341.t1 MTR_3g065980 34.085 399 218 11 144 521 166 540 2.48e-66 224
MS.gene033341.t1 MTR_2g082090 32.376 383 237 5 147 520 209 578 5.05e-63 216
MS.gene033341.t1 MTR_3g089055 32.723 382 235 5 149 521 197 565 6.87e-62 213
MS.gene033341.t1 MTR_3g089055 32.723 382 235 5 149 521 202 570 7.82e-62 213
MS.gene033341.t1 MTR_1g029420 32.461 382 236 5 149 521 224 592 9.85e-62 213
MS.gene033341.t1 MTR_5g097480 32.292 384 238 6 147 521 174 544 2.44e-60 208
MS.gene033341.t1 MTR_5g094450 30.928 388 242 7 147 521 158 532 6.65e-58 201
MS.gene033341.t1 MTR_4g064200 32.642 386 231 9 147 520 281 649 2.24e-57 202
MS.gene033341.t1 MTR_4g102790 34.109 387 234 12 144 520 286 661 1.56e-56 200
MS.gene033341.t1 MTR_3g056110 30.208 384 246 5 147 521 172 542 1.71e-56 197
MS.gene033341.t1 MTR_4g064160 29.948 384 246 6 147 520 313 683 2.39e-56 200
MS.gene033341.t1 MTR_4g064120 29.563 389 243 8 147 519 251 624 4.73e-53 190
MS.gene033341.t1 MTR_2g097467 30.130 385 241 7 147 520 286 653 5.04e-53 190
MS.gene033341.t1 MTR_4g064150 28.960 404 256 8 133 520 344 732 1.66e-52 190
MS.gene033341.t1 MTR_4g064180 29.049 389 245 8 147 519 251 624 7.71e-52 187
MS.gene033341.t1 MTR_2g097410 30.198 404 245 10 135 520 356 740 1.72e-51 187
MS.gene033341.t1 MTR_7g062120 29.870 385 247 6 147 521 369 740 2.18e-51 187
MS.gene033341.t1 MTR_4g133660 30.670 388 246 7 145 522 170 544 2.38e-51 184
MS.gene033341.t1 MTR_2g026250 31.458 391 239 9 145 522 223 597 4.59e-51 184
MS.gene033341.t1 MTR_7g074650 31.622 370 232 8 154 521 442 792 4.63e-50 184
MS.gene033341.t1 MTR_4g076140 28.345 441 240 10 144 521 42 469 9.98e-50 177
MS.gene033341.t1 MTR_2g097463 27.830 424 278 7 108 520 248 654 5.24e-49 179
MS.gene033341.t1 MTR_2g097473 27.594 424 279 7 108 520 247 653 6.02e-49 179
MS.gene033341.t1 MTR_2g097390 28.272 382 253 8 147 520 272 640 2.87e-47 174
MS.gene033341.t1 MTR_2g097310 28.982 383 249 10 147 520 269 637 2.49e-46 171
MS.gene033341.t1 MTR_2g097350 27.749 382 255 8 147 520 271 639 2.71e-46 171
MS.gene033341.t1 MTR_1g096030 30.244 410 234 15 142 521 72 459 9.64e-46 167
MS.gene033341.t1 MTR_4g076020 31.989 372 219 11 154 521 88 429 1.34e-44 163
MS.gene033341.t1 MTR_1g069725 29.302 430 261 13 113 522 58 464 1.69e-44 163
MS.gene033341.t1 MTR_2g034250 29.136 405 265 9 128 521 192 585 4.50e-44 164
MS.gene033341.t1 MTR_3g072710 30.732 410 251 11 134 522 104 501 5.20e-44 163
MS.gene033341.t1 MTR_4g077760 27.532 385 250 7 147 521 190 555 1.06e-42 160
MS.gene033341.t1 MTR_2g034260 29.177 401 255 12 134 521 200 584 2.93e-42 159
MS.gene033341.t1 MTR_5g058860 29.440 411 259 10 131 522 100 498 4.72e-41 154
MS.gene033341.t1 MTR_3g022830 26.241 423 248 6 151 521 26 436 7.81e-41 152
MS.gene033341.t1 MTR_1g086970 30.179 391 246 13 145 521 101 478 2.43e-40 152
MS.gene033341.t1 MTR_2g097380 26.005 373 228 8 155 520 229 560 1.19e-39 152
MS.gene033341.t1 MTR_2g034280 28.571 399 258 12 134 521 193 575 1.49e-39 151
MS.gene033341.t1 MTR_4g097080 26.221 389 252 10 150 521 133 503 3.84e-36 141
MS.gene033341.t1 MTR_7g109580 26.500 400 272 11 128 521 171 554 4.14e-36 141
MS.gene033341.t1 MTR_7g057230 30.592 304 199 6 225 520 357 656 1.61e-34 138
MS.gene033341.t1 MTR_5g009080 39.216 153 90 2 372 522 328 479 8.29e-34 134
MS.gene033341.t1 MTR_5g009080 31.982 222 139 5 144 365 50 259 6.32e-22 99.4
MS.gene033341.t1 MTR_5g015490 25.575 391 258 10 150 521 114 490 4.12e-33 132
MS.gene033341.t1 MTR_2g089100 25.829 422 260 11 133 520 40 442 2.52e-31 126
MS.gene033341.t1 MTR_6g047750 30.102 392 232 15 149 521 294 662 1.40e-30 126
MS.gene033341.t1 MTR_5g015950 25.891 421 254 13 149 520 34 445 1.45e-26 112
MS.gene033341.t1 MTR_3g053270 25.891 421 254 13 149 520 34 445 1.45e-26 112
MS.gene033341.t1 MTR_2g099110 24.824 427 267 14 122 521 52 451 2.05e-26 112
MS.gene033341.t1 MTR_4g095500 26.047 430 240 14 149 520 58 467 1.20e-25 110
MS.gene033341.t1 MTR_0092s0100 25.389 386 253 11 147 522 372 732 2.01e-22 101
MS.gene033341.t1 MTR_0092s0100 25.389 386 253 11 147 522 369 729 2.09e-22 101
MS.gene033341.t1 MTR_8g093070 27.366 391 236 16 147 520 136 495 5.99e-22 99.4
MS.gene033341.t1 MTR_4g026485 24.937 397 260 12 137 522 256 625 9.78e-22 99.4
MS.gene033341.t1 MTR_8g020840 24.146 410 255 13 143 521 164 548 7.34e-20 93.2
MS.gene033341.t1 MTR_3g022580 34.109 129 80 2 398 521 55 183 1.01e-17 81.6
MS.gene033341.t1 MTR_3g022005 34.653 101 66 0 421 521 25 125 9.88e-16 74.3
MS.gene033341.t1 MTR_8g077940 25.000 304 198 11 232 522 256 542 3.37e-15 78.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033341.t1 AT1G66350 36.554 383 215 10 144 522 149 507 7.56e-74 243
MS.gene033341.t1 AT1G14920 36.132 393 215 11 140 521 162 529 1.95e-69 232
MS.gene033341.t1 AT3G03450 35.294 391 218 12 144 521 177 545 2.68e-69 232
MS.gene033341.t1 AT5G17490 35.990 389 212 13 144 521 154 516 6.86e-69 230
MS.gene033341.t1 AT2G01570 34.359 390 220 11 143 521 217 581 3.66e-66 224
MS.gene033341.t1 AT5G48150 33.245 379 234 5 151 521 123 490 1.59e-63 215
MS.gene033341.t1 AT5G48150 33.245 379 234 5 151 521 123 490 1.59e-63 215
MS.gene033341.t1 AT5G48150 33.245 379 234 5 151 521 123 490 1.59e-63 215
MS.gene033341.t1 AT5G48150 33.245 379 234 5 151 521 123 490 1.59e-63 215
MS.gene033341.t1 AT1G21450 32.637 383 235 6 149 521 224 593 8.97e-61 210
MS.gene033341.t1 AT2G04890 31.733 375 239 5 150 521 53 413 5.82e-59 201
MS.gene033341.t1 AT1G50420 31.066 441 231 10 144 522 51 480 4.08e-58 201
MS.gene033341.t1 AT2G29065 32.134 389 233 10 150 520 252 627 9.07e-57 200
MS.gene033341.t1 AT1G50600 32.031 384 239 6 147 521 156 526 3.82e-56 196
MS.gene033341.t1 AT1G50600 32.031 384 239 6 147 521 227 597 9.61e-56 197
MS.gene033341.t1 AT1G50600 32.031 384 239 6 147 521 231 601 1.34e-55 196
MS.gene033341.t1 AT1G07520 31.620 389 234 10 150 520 154 528 2.57e-54 191
MS.gene033341.t1 AT1G07520 31.620 389 234 10 150 520 154 528 2.57e-54 191
MS.gene033341.t1 AT3G54220 34.054 370 224 9 154 520 297 649 7.88e-54 192
MS.gene033341.t1 AT1G07520 31.620 389 234 10 150 520 318 692 1.32e-53 192
MS.gene033341.t1 AT1G07520 31.620 389 234 10 150 520 319 693 1.51e-53 192
MS.gene033341.t1 AT3G46600 30.729 384 238 8 150 521 4 371 3.90e-53 184
MS.gene033341.t1 AT5G41920 31.762 403 253 10 123 522 18 401 9.52e-53 184
MS.gene033341.t1 AT5G59450 30.670 388 243 8 147 521 224 598 2.65e-52 187
MS.gene033341.t1 AT3G46600 30.729 384 238 8 150 521 212 579 1.36e-51 185
MS.gene033341.t1 AT3G46600 30.729 384 238 8 150 521 180 547 1.89e-51 184
MS.gene033341.t1 AT4G17230 31.606 386 242 6 145 521 153 525 1.17e-50 181
MS.gene033341.t1 AT4G17230 31.606 386 242 6 145 521 153 525 1.17e-50 181
MS.gene033341.t1 AT1G07530 28.385 384 253 5 147 520 393 764 1.57e-50 185
MS.gene033341.t1 AT2G37650 29.457 387 244 8 147 521 332 701 2.91e-49 181
MS.gene033341.t1 AT2G37650 29.457 387 244 8 147 521 344 713 3.13e-49 181
MS.gene033341.t1 AT2G29060 29.208 404 252 10 134 520 268 654 2.21e-48 177
MS.gene033341.t1 AT2G29060 29.208 404 252 10 134 520 305 691 3.69e-48 177
MS.gene033341.t1 AT5G66770 28.052 385 250 7 147 521 217 584 7.02e-45 167
MS.gene033341.t1 AT1G55580 34.680 297 176 6 239 521 153 445 7.68e-45 164
MS.gene033341.t1 AT1G63100 29.592 392 246 8 147 520 274 653 4.24e-44 166
MS.gene033341.t1 AT1G63100 29.592 392 246 8 147 520 249 628 4.71e-44 165
MS.gene033341.t1 AT3G50650 29.132 357 217 7 181 521 206 542 3.92e-41 155
MS.gene033341.t1 AT5G52510 29.808 312 191 8 233 521 334 640 3.22e-35 140
MS.gene033341.t1 AT4G08250 28.244 393 249 12 144 520 101 476 3.82e-34 135
MS.gene033341.t1 AT4G37650 26.551 403 246 14 150 521 146 529 3.19e-32 130
MS.gene033341.t1 AT3G49950 26.290 407 253 10 141 520 21 407 1.81e-28 117
MS.gene033341.t1 AT2G45160 26.111 360 216 14 182 522 312 640 1.52e-24 108
MS.gene033341.t1 AT3G60630 27.476 313 188 13 231 522 329 623 3.14e-23 103
MS.gene033341.t1 AT4G00150 24.860 358 223 12 189 522 220 555 2.41e-21 97.8
MS.gene033341.t1 AT4G36710 25.604 414 255 17 127 521 106 485 8.30e-19 89.7
MS.gene033341.t1 AT3G13840 25.061 411 252 16 134 521 133 510 1.26e-13 73.6

Find 96 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATTGTTGAGAAGAATT+TGG 0.186388 8.4:-21803441 MS.gene033341:CDS
AATTGTCCACATATTCAATT+TGG 0.204871 8.4:-21803719 MS.gene033341:CDS
CAACCGTAACTATCCCTATT+TGG 0.207024 8.4:-21804234 MS.gene033341:CDS
CTGAAATTCGCAAACCAAAT+AGG 0.213265 8.4:+21804220 None:intergenic
CTCAAAGGCTTCCAATATTA+TGG 0.226205 8.4:+21803679 None:intergenic
TGAAAATCCATCATCATTAA+TGG 0.252428 8.4:-21804386 MS.gene033341:CDS
TGTGGCTAATTCCATAATAT+TGG 0.269201 8.4:-21803690 MS.gene033341:CDS
GAGAAAGGGTGTTTGGTTCT+TGG 0.272544 8.4:-21802930 MS.gene033341:CDS
CATGATCAGAAAATGGATTA+AGG 0.277342 8.4:+21804296 None:intergenic
AAATCAAATGCTTTGGTGTT+TGG 0.284256 8.4:-21803911 MS.gene033341:CDS
GCTTGAACAACCATCTTAAT+AGG 0.288790 8.4:+21803005 None:intergenic
TCAGTTTCATGATCAGAAAA+TGG 0.293107 8.4:+21804289 None:intergenic
TTCTGCTATATTTGACTCAT+TGG 0.296218 8.4:-21803195 MS.gene033341:CDS
TATTGAAGAAACTATTACTA+TGG 0.299092 8.4:+21804194 None:intergenic
GTTATGGCTGAGCAAGATTC+TGG 0.301485 8.4:-21803269 MS.gene033341:CDS
TTTCTTCATGTGGTTGATCT+TGG 0.314063 8.4:-21803647 MS.gene033341:CDS
TTCATCTACCTCACATTTGA+AGG 0.343224 8.4:+21804405 None:intergenic
CAGCAAGAAACTGCAACAAT+AGG 0.367426 8.4:+21802894 None:intergenic
CTATTGTTGCAGTTTCTTGC+TGG 0.367470 8.4:-21802893 MS.gene033341:CDS
CATAATATTGGAAGCCTTTG+AGG 0.384786 8.4:-21803678 MS.gene033341:CDS
TGAAATTCGCAAACCAAATA+GGG 0.387079 8.4:+21804221 None:intergenic
ATCGATCCAGCCACCGTGTT+CGG 0.388370 8.4:-21803565 MS.gene033341:CDS
GGATGCTGCATCAGAAGAAA+TGG 0.398391 8.4:-21803768 MS.gene033341:CDS
CCAGCAGAGTTATTACCAAT+TGG 0.412468 8.4:+21803821 None:intergenic
AAAATCAATGATGAACATAA+TGG 0.412525 8.4:-21803789 MS.gene033341:CDS
CGAGCTTAAATCAAATGCTT+TGG 0.422403 8.4:-21803918 MS.gene033341:CDS
GAAAATCCATCATCATTAAT+GGG 0.422874 8.4:-21804385 MS.gene033341:CDS
GTTGATCTTGGCATGAGCCT+TGG 0.423272 8.4:-21803635 MS.gene033341:CDS
TCAGCCATAACCAACACTTT+TGG 0.423891 8.4:+21803281 None:intergenic
AGGAGGAGAATGAGTAGGGC+TGG 0.435845 8.4:-21803041 MS.gene033341:CDS
TTCGCCAAAAGTGTTGGTTA+TGG 0.438665 8.4:-21803285 MS.gene033341:CDS
ATGAGTAGGGCTGGATTTCA+AGG 0.440915 8.4:-21803032 MS.gene033341:CDS
AAGCTGCCGAACACGGTGGC+TGG 0.447890 8.4:+21803559 None:intergenic
AAGGGTGTTTGGTTCTTGGA+TGG 0.458218 8.4:-21802926 MS.gene033341:CDS
TTTGGAGAATTTGAAACCTA+AGG 0.459147 8.4:-21803423 MS.gene033341:CDS
TCATGCCTCTCCATCCTCAA+TGG 0.459152 8.4:+21803080 None:intergenic
TAAGAACCCATTAATGATGA+TGG 0.459556 8.4:+21804379 None:intergenic
TCAAGGTTCACCTATTAAGA+TGG 0.464234 8.4:-21803015 MS.gene033341:CDS
CTAACTCTGATTCAACCAAT+TGG 0.465189 8.4:-21803836 MS.gene033341:CDS
TGTGGAAGAGAAAGGGTGTT+TGG 0.469216 8.4:-21802937 MS.gene033341:CDS
GCCACCGTGTTCGGCAGCTT+AGG 0.471055 8.4:-21803556 MS.gene033341:CDS
GCCCTCTTAGTGTCATACTT+TGG 0.484118 8.4:+21803161 None:intergenic
AAATGTCCAAATTGAATATG+TGG 0.495894 8.4:+21803713 None:intergenic
TGCCAAAGTATGACACTAAG+AGG 0.499635 8.4:-21803163 MS.gene033341:CDS
TATACTGTTGTGGAAGAGAA+AGG 0.500286 8.4:-21802945 MS.gene033341:CDS
AGAGTTCTTCACCAATGACT+TGG 0.501290 8.4:+21803501 None:intergenic
TCGCGACAAATCACACGCTT+CGG 0.504012 8.4:-21803951 MS.gene033341:CDS
ATAATATTGGAAGCCTTTGA+GGG 0.506937 8.4:-21803677 MS.gene033341:CDS
CCAATTGGTAATAACTCTGC+TGG 0.507639 8.4:-21803821 MS.gene033341:CDS
AAGAACATTGTGAGCTGTGA+AGG 0.508905 8.4:-21803104 MS.gene033341:CDS
CAATTTCTTGTGATCCCTAG+TGG 0.519092 8.4:+21804264 None:intergenic
ATTGCTAGAATCCAAGTCAT+TGG 0.519171 8.4:-21803512 MS.gene033341:CDS
TGATCTCTGAAATGAAGCCT+TGG 0.520426 8.4:+21804136 None:intergenic
AGAAAGTTGATCAGTGGAGG+AGG 0.527630 8.4:-21803058 MS.gene033341:CDS
CAAAGCATTTGATTTAAGCT+CGG 0.529084 8.4:+21803919 None:intergenic
TTTGAATTAGTCCTCGCCAT+TGG 0.537234 8.4:+21803597 None:intergenic
ATACTGTTGTGGAAGAGAAA+GGG 0.539638 8.4:-21802944 MS.gene033341:CDS
AAGAAAACTCTCACCCTCAA+AGG 0.545146 8.4:+21803664 None:intergenic
AATGGAAGAAGCATTCAAGT+TGG 0.548963 8.4:-21803750 MS.gene033341:CDS
ATGATTCCATAAACCTTCCA+AGG 0.552621 8.4:+21803228 None:intergenic
AGTGGAGGAGGAGAATGAGT+AGG 0.556868 8.4:-21803046 MS.gene033341:CDS
CATAAAAGTGAATGAAAATG+AGG 0.557353 8.4:-21803399 MS.gene033341:CDS
GTGAAGAATAATGTTTGTGA+TGG 0.562851 8.4:-21802969 MS.gene033341:CDS
GTGGAGGAGGAGAATGAGTA+GGG 0.563946 8.4:-21803045 MS.gene033341:CDS
GGCATGAGAAAGTTGATCAG+TGG 0.565335 8.4:-21803064 MS.gene033341:CDS
AATTGCGAAGCTGAAGAAGA+TGG 0.566408 8.4:-21804037 MS.gene033341:CDS
CGGTCTTTCGCCAAAAGTGT+TGG 0.568749 8.4:-21803291 MS.gene033341:CDS
TGCTAGTACCTTCAAATGTG+AGG 0.569925 8.4:-21804413 MS.gene033341:CDS
GTCTTCCACATGGACACCAA+TGG 0.579517 8.4:-21803613 MS.gene033341:CDS
AAACCAAATAGGGATAGTTA+CGG 0.582805 8.4:+21804231 None:intergenic
CAGCTTAGGATCACAGCGAT+TGG 0.586462 8.4:-21803542 MS.gene033341:CDS
GAAGATGGTTCATGTGCTGA+TGG 0.596375 8.4:-21804022 MS.gene033341:CDS
AAGGTACTAGCATAAAGTGC+AGG 0.596660 8.4:+21804424 None:intergenic
ATGAGAAAGTTGATCAGTGG+AGG 0.606341 8.4:-21803061 MS.gene033341:CDS
TGATCCTAAGCTGCCGAACA+CGG 0.610159 8.4:+21803552 None:intergenic
ATCATGAAACTGAAACCACT+AGG 0.610327 8.4:-21804279 MS.gene033341:CDS
CTGTGAAGGACCATTGAGGA+TGG 0.613485 8.4:-21803090 MS.gene033341:CDS
GAGTCGTGGCGCTTTGAATG+CGG 0.617247 8.4:-21803330 MS.gene033341:CDS
AAGGACCATTGAGGATGGAG+AGG 0.621814 8.4:-21803085 MS.gene033341:CDS
TCATGAAACTGAAACCACTA+GGG 0.631575 8.4:-21804278 MS.gene033341:CDS
ATGAGCCTTGGTCTTCCACA+TGG 0.634567 8.4:-21803623 MS.gene033341:CDS
AAAACAGTGCTAGTTCAACA+TGG 0.638515 8.4:-21804321 MS.gene033341:CDS
GAGCAAGATTCTGGTCACAA+TGG 0.639301 8.4:-21803260 MS.gene033341:CDS
TTGTGATGGATATACTGTTG+TGG 0.639465 8.4:-21802955 MS.gene033341:CDS
TGGTTGTTCAAGCTAAGCAG+TGG 0.641582 8.4:-21802995 MS.gene033341:CDS
ACAGTGTGAATAGCATTCCA+AGG 0.646338 8.4:-21804153 MS.gene033341:CDS
GGTGTCCATGTGGAAGACCA+AGG 0.647949 8.4:+21803618 None:intergenic
CCTCGCCATTGGTGTCCATG+TGG 0.649204 8.4:+21803608 None:intergenic
GCCAAAGTATGACACTAAGA+GGG 0.650623 8.4:-21803162 MS.gene033341:CDS
TGTGTTGTTAAAGAGAGTCG+TGG 0.651261 8.4:-21803344 MS.gene033341:CDS
CCACATGGACACCAATGGCG+AGG 0.669979 8.4:-21803608 MS.gene033341:CDS
GAATGAAAATGAGGTCCTTG+TGG 0.675244 8.4:-21803390 MS.gene033341:CDS
GAAGAATGCTGTTAACCACA+AGG 0.689238 8.4:+21803375 None:intergenic
AGGACTAATTCAAAGTCTCG+CGG 0.704228 8.4:-21803588 MS.gene033341:CDS
TGAGCTGTGAAGGACCATTG+AGG 0.705754 8.4:-21803094 MS.gene033341:CDS
TCCTAAGCTGCCGAACACGG+TGG 0.761080 8.4:+21803555 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCAATGATGAACATAA+TGG - chr8.4:21803525-21803544 MS.gene033341:CDS 20.0%
!! TATTGAAGAAACTATTACTA+TGG + chr8.4:21803123-21803142 None:intergenic 20.0%
!!! TTTTCTAAAATAGGCAAATT+TGG + chr8.4:21802977-21802996 None:intergenic 20.0%
! AAATGTCCAAATTGAATATG+TGG + chr8.4:21803604-21803623 None:intergenic 25.0%
! AATTGTCCACATATTCAATT+TGG - chr8.4:21803595-21803614 MS.gene033341:CDS 25.0%
! CATAAAAGTGAATGAAAATG+AGG - chr8.4:21803915-21803934 MS.gene033341:CDS 25.0%
! GAAAATCCATCATCATTAAT+GGG - chr8.4:21802929-21802948 MS.gene033341:CDS 25.0%
! TCAATTTACGCGAAAAATTT+GGG - chr8.4:21803835-21803854 MS.gene033341:CDS 25.0%
! TGAAAATCCATCATCATTAA+TGG - chr8.4:21802928-21802947 MS.gene033341:CDS 25.0%
!! TATTCAATTTGGACATTTTG+TGG - chr8.4:21803606-21803625 MS.gene033341:CDS 25.0%
AAACCAAATAGGGATAGTTA+CGG + chr8.4:21803086-21803105 None:intergenic 30.0%
ATAAACCTTCCAAGGAAAAA+AGG + chr8.4:21804081-21804100 None:intergenic 30.0%
ATAATATTGGAAGCCTTTGA+GGG - chr8.4:21803637-21803656 MS.gene033341:CDS 30.0%
CAATTTACGCGAAAAATTTG+GGG - chr8.4:21803836-21803855 MS.gene033341:CDS 30.0%
CTCAATTGTTGAGAAGAATT+TGG - chr8.4:21803873-21803892 MS.gene033341:CDS 30.0%
CTCAATTTACGCGAAAAATT+TGG - chr8.4:21803834-21803853 MS.gene033341:CDS 30.0%
GAAAAATTTGGGGATTCATT+TGG - chr8.4:21803846-21803865 MS.gene033341:CDS 30.0%
GTGAAGAATAATGTTTGTGA+TGG - chr8.4:21804345-21804364 MS.gene033341:CDS 30.0%
TAAGAACCCATTAATGATGA+TGG + chr8.4:21802938-21802957 None:intergenic 30.0%
TCAGTTTCATGATCAGAAAA+TGG + chr8.4:21803028-21803047 None:intergenic 30.0%
TGAAATTCGCAAACCAAATA+GGG + chr8.4:21803096-21803115 None:intergenic 30.0%
TGTGGCTAATTCCATAATAT+TGG - chr8.4:21803624-21803643 MS.gene033341:CDS 30.0%
TTCTGCTATATTTGACTCAT+TGG - chr8.4:21804119-21804138 MS.gene033341:CDS 30.0%
! CAAAGCATTTGATTTAAGCT+CGG + chr8.4:21803398-21803417 None:intergenic 30.0%
! TTTGGAGAATTTGAAACCTA+AGG - chr8.4:21803891-21803910 MS.gene033341:CDS 30.0%
!! AAATCAAATGCTTTGGTGTT+TGG - chr8.4:21803403-21803422 MS.gene033341:CDS 30.0%
!! CATGATCAGAAAATGGATTA+AGG + chr8.4:21803021-21803040 None:intergenic 30.0%
!! TCATTCACTTTTATGTCCTT+AGG + chr8.4:21803910-21803929 None:intergenic 30.0%
!!! CTAGCACTGTTTTCTAAAAT+AGG + chr8.4:21802986-21803005 None:intergenic 30.0%
!!! TTTTTTCCTTGGAAGGTTTA+TGG - chr8.4:21804080-21804099 MS.gene033341:CDS 30.0%
AAAACAGTGCTAGTTCAACA+TGG - chr8.4:21802993-21803012 MS.gene033341:CDS 35.0%
ATACTGTTGTGGAAGAGAAA+GGG - chr8.4:21804370-21804389 MS.gene033341:CDS 35.0%
ATCATGAAACTGAAACCACT+AGG - chr8.4:21803035-21803054 MS.gene033341:CDS 35.0%
ATGATTCCATAAACCTTCCA+AGG + chr8.4:21804089-21804108 None:intergenic 35.0%
ATTGCTAGAATCCAAGTCAT+TGG - chr8.4:21803802-21803821 MS.gene033341:CDS 35.0%
CATAATATTGGAAGCCTTTG+AGG - chr8.4:21803636-21803655 MS.gene033341:CDS 35.0%
CGAGCTTAAATCAAATGCTT+TGG - chr8.4:21803396-21803415 MS.gene033341:CDS 35.0%
CTAACTCTGATTCAACCAAT+TGG - chr8.4:21803478-21803497 MS.gene033341:CDS 35.0%
CTCAAAGGCTTCCAATATTA+TGG + chr8.4:21803638-21803657 None:intergenic 35.0%
CTGAAATTCGCAAACCAAAT+AGG + chr8.4:21803097-21803116 None:intergenic 35.0%
GCTTGAACAACCATCTTAAT+AGG + chr8.4:21804312-21804331 None:intergenic 35.0%
TATACTGTTGTGGAAGAGAA+AGG - chr8.4:21804369-21804388 MS.gene033341:CDS 35.0%
TCAAGGTTCACCTATTAAGA+TGG - chr8.4:21804299-21804318 MS.gene033341:CDS 35.0%
TCATGAAACTGAAACCACTA+GGG - chr8.4:21803036-21803055 MS.gene033341:CDS 35.0%
TTCATCTACCTCACATTTGA+AGG + chr8.4:21802912-21802931 None:intergenic 35.0%
TTTCTTCATGTGGTTGATCT+TGG - chr8.4:21803667-21803686 MS.gene033341:CDS 35.0%
!! AATGGAAGAAGCATTCAAGT+TGG - chr8.4:21803564-21803583 MS.gene033341:CDS 35.0%
!! TTGTGATGGATATACTGTTG+TGG - chr8.4:21804359-21804378 MS.gene033341:CDS 35.0%
!!! GGAACAGTTTTATTTTGCTG+AGG - chr8.4:21804182-21804201 MS.gene033341:CDS 35.0%
!!! GTTGCTTCTTGTTTTGTTCA+AGG - chr8.4:21803442-21803461 MS.gene033341:CDS 35.0%
AAGAAAACTCTCACCCTCAA+AGG + chr8.4:21803653-21803672 None:intergenic 40.0%
AAGAACATTGTGAGCTGTGA+AGG - chr8.4:21804210-21804229 MS.gene033341:CDS 40.0%
AATTGCGAAGCTGAAGAAGA+TGG - chr8.4:21803277-21803296 MS.gene033341:CDS 40.0%
ACAGTGTGAATAGCATTCCA+AGG - chr8.4:21803161-21803180 MS.gene033341:CDS 40.0%
AGAGTTCTTCACCAATGACT+TGG + chr8.4:21803816-21803835 None:intergenic 40.0%
AGGACTAATTCAAAGTCTCG+CGG - chr8.4:21803726-21803745 MS.gene033341:CDS 40.0%
CAACCGTAACTATCCCTATT+TGG - chr8.4:21803080-21803099 MS.gene033341:CDS 40.0%
CAATTTCTTGTGATCCCTAG+TGG + chr8.4:21803053-21803072 None:intergenic 40.0%
CAGCAAGAAACTGCAACAAT+AGG + chr8.4:21804423-21804442 None:intergenic 40.0%
CCAGCAGAGTTATTACCAAT+TGG + chr8.4:21803496-21803515 None:intergenic 40.0%
CTATTGTTGCAGTTTCTTGC+TGG - chr8.4:21804421-21804440 MS.gene033341:CDS 40.0%
GAAGAATGCTGTTAACCACA+AGG + chr8.4:21803942-21803961 None:intergenic 40.0%
GAATGAAAATGAGGTCCTTG+TGG - chr8.4:21803924-21803943 MS.gene033341:CDS 40.0%
GCCAAAGTATGACACTAAGA+GGG - chr8.4:21804152-21804171 MS.gene033341:CDS 40.0%
TGACACTAAGAGGGCAAAAA+TGG - chr8.4:21804161-21804180 MS.gene033341:CDS 40.0%
TGCCAAAGTATGACACTAAG+AGG - chr8.4:21804151-21804170 MS.gene033341:CDS 40.0%
TGCTAGTACCTTCAAATGTG+AGG - chr8.4:21802901-21802920 MS.gene033341:CDS 40.0%
TTTGAATTAGTCCTCGCCAT+TGG + chr8.4:21803720-21803739 None:intergenic 40.0%
! AAGGTACTAGCATAAAGTGC+AGG + chr8.4:21802893-21802912 None:intergenic 40.0%
! ATGAGAAAGTTGATCAGTGG+AGG - chr8.4:21804253-21804272 MS.gene033341:CDS 40.0%
! CCAATTGGTAATAACTCTGC+TGG - chr8.4:21803493-21803512 MS.gene033341:CDS 40.0%
! GCGGTTTTACAAATGATCCA+CGG - chr8.4:21804003-21804022 MS.gene033341:CDS 40.0%
! TCAGCCATAACCAACACTTT+TGG + chr8.4:21804036-21804055 None:intergenic 40.0%
! TGTGTTGTTAAAGAGAGTCG+TGG - chr8.4:21803970-21803989 MS.gene033341:CDS 40.0%
!! TGATCTCTGAAATGAAGCCT+TGG + chr8.4:21803181-21803200 None:intergenic 40.0%
!! TTCGCCAAAAGTGTTGGTTA+TGG - chr8.4:21804029-21804048 MS.gene033341:CDS 40.0%
!!! ATGGACCTTTTTTCCTTGGA+AGG - chr8.4:21804073-21804092 MS.gene033341:CDS 40.0%
!!! CACAATGGACCTTTTTTCCT+TGG - chr8.4:21804069-21804088 MS.gene033341:CDS 40.0%
ATGAGTAGGGCTGGATTTCA+AGG - chr8.4:21804282-21804301 MS.gene033341:CDS 45.0%
GAAGATGGTTCATGTGCTGA+TGG - chr8.4:21803292-21803311 MS.gene033341:CDS 45.0%
GAGCAAGATTCTGGTCACAA+TGG - chr8.4:21804054-21804073 MS.gene033341:CDS 45.0%
GCCCTCTTAGTGTCATACTT+TGG + chr8.4:21804156-21804175 None:intergenic 45.0%
GGATGCTGCATCAGAAGAAA+TGG - chr8.4:21803546-21803565 MS.gene033341:CDS 45.0%
GTTATGGCTGAGCAAGATTC+TGG - chr8.4:21804045-21804064 MS.gene033341:CDS 45.0%
TGGTTGTTCAAGCTAAGCAG+TGG - chr8.4:21804319-21804338 MS.gene033341:CDS 45.0%
! AGAAAGTTGATCAGTGGAGG+AGG - chr8.4:21804256-21804275 MS.gene033341:CDS 45.0%
! GGCATGAGAAAGTTGATCAG+TGG - chr8.4:21804250-21804269 MS.gene033341:CDS 45.0%
! GGGTGAGAGTTTTCTTCATG+TGG - chr8.4:21803657-21803676 MS.gene033341:CDS 45.0%
!! AAGGGTGTTTGGTTCTTGGA+TGG - chr8.4:21804388-21804407 MS.gene033341:CDS 45.0%
!! GAGAAAGGGTGTTTGGTTCT+TGG - chr8.4:21804384-21804403 MS.gene033341:CDS 45.0%
!! TGTGGAAGAGAAAGGGTGTT+TGG - chr8.4:21804377-21804396 MS.gene033341:CDS 45.0%
AAGGACCATTGAGGATGGAG+AGG - chr8.4:21804229-21804248 MS.gene033341:CDS 50.0%
AGTGGAGGAGGAGAATGAGT+AGG - chr8.4:21804268-21804287 MS.gene033341:CDS 50.0%
CAGCTTAGGATCACAGCGAT+TGG - chr8.4:21803772-21803791 MS.gene033341:CDS 50.0%
CTGTGAAGGACCATTGAGGA+TGG - chr8.4:21804224-21804243 MS.gene033341:CDS 50.0%
GTCTTCCACATGGACACCAA+TGG - chr8.4:21803701-21803720 MS.gene033341:CDS 50.0%
GTGGAGGAGGAGAATGAGTA+GGG - chr8.4:21804269-21804288 MS.gene033341:CDS 50.0%
GTTGATCTTGGCATGAGCCT+TGG - chr8.4:21803679-21803698 MS.gene033341:CDS 50.0%
TCATGCCTCTCCATCCTCAA+TGG + chr8.4:21804237-21804256 None:intergenic 50.0%
TCGCGACAAATCACACGCTT+CGG - chr8.4:21803363-21803382 MS.gene033341:CDS 50.0%
TGAGCTGTGAAGGACCATTG+AGG - chr8.4:21804220-21804239 MS.gene033341:CDS 50.0%
TGATCCTAAGCTGCCGAACA+CGG + chr8.4:21803765-21803784 None:intergenic 50.0%
! ACACTTTTGGCGAAAGACCG+TGG + chr8.4:21804023-21804042 None:intergenic 50.0%
! ATGAGCCTTGGTCTTCCACA+TGG - chr8.4:21803691-21803710 MS.gene033341:CDS 50.0%
! CGGTCTTTCGCCAAAAGTGT+TGG - chr8.4:21804023-21804042 MS.gene033341:CDS 50.0%
AGGAGGAGAATGAGTAGGGC+TGG - chr8.4:21804273-21804292 MS.gene033341:CDS 55.0%
ATCGATCCAGCCACCGTGTT+CGG - chr8.4:21803749-21803768 MS.gene033341:CDS 55.0%
GGTGTCCATGTGGAAGACCA+AGG + chr8.4:21803699-21803718 None:intergenic 55.0%
! GAGTCGTGGCGCTTTGAATG+CGG - chr8.4:21803984-21804003 MS.gene033341:CDS 55.0%
CCACATGGACACCAATGGCG+AGG - chr8.4:21803706-21803725 MS.gene033341:CDS 60.0%
TCCTAAGCTGCCGAACACGG+TGG + chr8.4:21803762-21803781 None:intergenic 60.0%
! CCTCGCCATTGGTGTCCATG+TGG + chr8.4:21803709-21803728 None:intergenic 60.0%
AAGCTGCCGAACACGGTGGC+TGG + chr8.4:21803758-21803777 None:intergenic 65.0%
GCCACCGTGTTCGGCAGCTT+AGG - chr8.4:21803758-21803777 MS.gene033341:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 21802884 21804452 21802884 ID=MS.gene033341
chr8.4 mRNA 21802884 21804452 21802884 ID=MS.gene033341.t1;Parent=MS.gene033341
chr8.4 exon 21802884 21804452 21802884 ID=MS.gene033341.t1.exon1;Parent=MS.gene033341.t1
chr8.4 CDS 21802884 21804452 21802884 ID=cds.MS.gene033341.t1;Parent=MS.gene033341.t1
Gene Sequence

>MS.gene033341

ATGTCACCTGCACTTTATGCTAGTACCTTCAAATGTGAGGTAGATGAAAATCCATCATCATTAATGGGTTCTTACTATGCTTCTTTATATCCAAATTTGCCTATTTTAGAAAACAGTGCTAGTTCAACATGGATCCTTAATCCATTTTCTGATCATGAAACTGAAACCACTAGGGATCACAAGAAATTGAAAAGATCAACCGTAACTATCCCTATTTGGTTTGCGAATTTCAGCAGCCATAGTAATAGTTTCTTCAATAACATCAACAGCAATAACAACAGTGTGAATAGCATTCCAAGGCTTCATTTCAGAGATCACATAAGAACTTATAAGCAAAGATATTTTGCTGCTGAAGCTGTTGAAGAAGCAGCAGAAGATAACTTCAACTACAATAATTGCGAAGCTGAAGAAGATGGTTCATGTGCTGATGGAATGAGACTAGTTCAACTTCTAATTGCATGTGCTGAAGCTGTCGCGTGTCGCGACAAATCACACGCTTCGGTTTTACTGTCCGAGCTTAAATCAAATGCTTTGGTGTTTGGTTCTTCATTTCAAAGAGTTGCTTCTTGTTTTGTTCAAGGACTCACAGAGAGACTAACTCTGATTCAACCAATTGGTAATAACTCTGCTGGATCAGATACAAAATCAATGATGAACATAATGGATGCTGCATCAGAAGAAATGGAAGAAGCATTCAAGTTGGTTTATGAAAATTGTCCACATATTCAATTTGGACATTTTGTGGCTAATTCCATAATATTGGAAGCCTTTGAGGGTGAGAGTTTTCTTCATGTGGTTGATCTTGGCATGAGCCTTGGTCTTCCACATGGACACCAATGGCGAGGACTAATTCAAAGTCTCGCGGATCGATCCAGCCACCGTGTTCGGCAGCTTAGGATCACAGCGATTGGTCTATGCATTGCTAGAATCCAAGTCATTGGTGAAGAACTCTCAATTTACGCGAAAAATTTGGGGATTCATTTGGAATTCTCAATTGTTGAGAAGAATTTGGAGAATTTGAAACCTAAGGACATAAAAGTGAATGAAAATGAGGTCCTTGTGGTTAACAGCATTCTTCAATTGCATTGTGTTGTTAAAGAGAGTCGTGGCGCTTTGAATGCGGTTTTACAAATGATCCACGGTCTTTCGCCAAAAGTGTTGGTTATGGCTGAGCAAGATTCTGGTCACAATGGACCTTTTTTCCTTGGAAGGTTTATGGAATCATTGCATTATTATTCTGCTATATTTGACTCATTGGATGCTATGCTGCCAAAGTATGACACTAAGAGGGCAAAAATGGAACAGTTTTATTTTGCTGAGGAGATAAAGAACATTGTGAGCTGTGAAGGACCATTGAGGATGGAGAGGCATGAGAAAGTTGATCAGTGGAGGAGGAGAATGAGTAGGGCTGGATTTCAAGGTTCACCTATTAAGATGGTTGTTCAAGCTAAGCAGTGGCTTGTGAAGAATAATGTTTGTGATGGATATACTGTTGTGGAAGAGAAAGGGTGTTTGGTTCTTGGATGGAAATCAAAGCCTATTGTTGCAGTTTCTTGCTGGAAATGCTGA
Protein sequence

>MS.gene033341.t1

MSPALYASTFKCEVDENPSSLMGSYYASLYPNLPILENSASSTWILNPFSDHETETTRDHKKLKRSTVTIPIWFANFSSHSNSFFNNINSNNNSVNSIPRLHFRDHIRTYKQRYFAAEAVEEAAEDNFNYNNCEAEEDGSCADGMRLVQLLIACAEAVACRDKSHASVLLSELKSNALVFGSSFQRVASCFVQGLTERLTLIQPIGNNSAGSDTKSMMNIMDAASEEMEEAFKLVYENCPHIQFGHFVANSIILEAFEGESFLHVVDLGMSLGLPHGHQWRGLIQSLADRSSHRVRQLRITAIGLCIARIQVIGEELSIYAKNLGIHLEFSIVEKNLENLKPKDIKVNENEVLVVNSILQLHCVVKESRGALNAVLQMIHGLSPKVLVMAEQDSGHNGPFFLGRFMESLHYYSAIFDSLDAMLPKYDTKRAKMEQFYFAEEIKNIVSCEGPLRMERHEKVDQWRRRMSRAGFQGSPIKMVVQAKQWLVKNNVCDGYTVVEEKGCLVLGWKSKPIVAVSCWKC