AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033387

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033387.t1 MTR_4g102790 98.380 679 8 2 1 678 1 677 0.0 1374
MS.gene033387.t1 MTR_7g027190 37.722 395 223 7 281 662 288 672 7.34e-81 270
MS.gene033387.t1 MTR_7g074650 36.856 369 215 10 297 664 442 793 3.64e-63 224
MS.gene033387.t1 MTR_1g069725 33.810 420 247 11 256 663 63 463 1.10e-58 205
MS.gene033387.t1 MTR_5g094450 33.418 395 227 10 286 662 155 531 5.29e-58 205
MS.gene033387.t1 MTR_4g104020 34.367 387 233 13 287 662 143 519 1.03e-56 201
MS.gene033387.t1 MTR_8g442410 29.259 499 317 12 185 662 41 524 2.83e-56 200
MS.gene033387.t1 MTR_3g065980 31.328 399 236 8 282 662 161 539 1.33e-55 199
MS.gene033387.t1 MTR_3g089055 36.126 382 217 14 293 662 198 564 1.77e-55 199
MS.gene033387.t1 MTR_3g089055 36.126 382 217 14 293 662 203 569 2.06e-55 199
MS.gene033387.t1 MTR_1g096030 33.088 408 226 14 283 662 70 458 9.33e-55 194
MS.gene033387.t1 MTR_1g029420 35.079 382 221 13 293 662 225 591 4.56e-54 196
MS.gene033387.t1 MTR_4g076140 28.689 488 266 11 236 662 2 468 5.58e-54 192
MS.gene033387.t1 MTR_2g082090 32.282 412 244 13 269 662 184 578 4.40e-53 192
MS.gene033387.t1 MTR_5g097480 33.508 382 233 11 290 662 174 543 4.42e-53 192
MS.gene033387.t1 MTR_3g056110 30.769 403 249 9 273 662 156 541 8.98e-51 185
MS.gene033387.t1 MTR_4g076020 33.245 376 216 14 297 667 88 433 4.09e-47 172
MS.gene033387.t1 MTR_3g022830 26.777 422 244 7 294 662 26 435 4.43e-47 172
MS.gene033387.t1 MTR_2g097410 28.979 421 274 11 258 664 333 742 5.78e-46 175
MS.gene033387.t1 MTR_4g133660 29.610 385 249 9 288 662 170 542 5.12e-45 169
MS.gene033387.t1 MTR_2g097310 26.302 384 263 8 289 664 268 639 8.22e-45 170
MS.gene033387.t1 MTR_2g026250 28.319 452 299 12 221 662 159 595 1.06e-44 169
MS.gene033387.t1 MTR_2g097380 27.763 371 223 9 300 664 231 562 6.23e-44 166
MS.gene033387.t1 MTR_4g097080 27.249 389 251 9 292 664 132 504 7.92e-44 165
MS.gene033387.t1 MTR_4g077760 31.152 382 237 12 290 662 190 554 1.15e-43 166
MS.gene033387.t1 MTR_2g097390 26.684 386 259 9 289 664 271 642 2.09e-43 166
MS.gene033387.t1 MTR_4g064160 27.040 429 288 10 250 664 268 685 1.76e-41 161
MS.gene033387.t1 MTR_4g064120 27.404 416 274 11 266 664 223 627 6.31e-41 159
MS.gene033387.t1 MTR_4g064150 27.273 407 268 12 274 665 342 735 1.89e-40 158
MS.gene033387.t1 MTR_2g097463 24.031 516 326 12 197 664 159 656 2.00e-40 157
MS.gene033387.t1 MTR_5g009080 32.443 262 166 2 239 500 9 259 2.20e-40 154
MS.gene033387.t1 MTR_5g009080 33.333 153 95 5 511 662 331 477 6.97e-19 90.5
MS.gene033387.t1 MTR_5g058860 31.785 409 239 16 279 663 105 497 2.91e-40 155
MS.gene033387.t1 MTR_3g072710 31.127 408 237 16 283 663 110 500 3.09e-40 155
MS.gene033387.t1 MTR_2g097350 25.515 388 261 7 289 664 270 641 4.68e-40 156
MS.gene033387.t1 MTR_4g064180 27.525 396 263 10 282 664 243 627 4.85e-40 156
MS.gene033387.t1 MTR_5g015490 28.608 388 253 11 292 664 113 491 8.15e-40 153
MS.gene033387.t1 MTR_4g064200 26.494 385 261 9 289 664 280 651 1.18e-39 155
MS.gene033387.t1 MTR_2g034250 28.109 402 268 10 276 665 195 587 1.69e-39 154
MS.gene033387.t1 MTR_2g034280 27.820 399 264 12 276 662 188 574 1.89e-37 148
MS.gene033387.t1 MTR_2g034260 27.930 401 263 11 276 662 195 583 1.91e-37 148
MS.gene033387.t1 MTR_2g097467 24.936 389 265 9 287 664 283 655 3.15e-37 148
MS.gene033387.t1 MTR_7g062120 24.936 389 264 8 289 664 368 741 1.09e-36 147
MS.gene033387.t1 MTR_8g093070 29.383 405 241 16 278 666 124 499 2.68e-36 143
MS.gene033387.t1 MTR_7g109580 26.992 389 263 10 282 662 178 553 4.95e-36 143
MS.gene033387.t1 MTR_2g089100 25.616 406 261 10 293 667 52 447 1.43e-35 140
MS.gene033387.t1 MTR_2g097473 24.524 420 279 11 267 664 252 655 5.92e-35 141
MS.gene033387.t1 MTR_1g086970 24.510 408 290 11 264 662 79 477 3.34e-34 137
MS.gene033387.t1 MTR_2g099110 25.679 405 242 10 293 664 74 452 5.65e-32 130
MS.gene033387.t1 MTR_7g057230 34.221 263 160 10 405 662 402 656 2.65e-31 130
MS.gene033387.t1 MTR_5g015950 24.048 420 264 13 293 664 35 447 1.53e-25 110
MS.gene033387.t1 MTR_3g053270 24.048 420 264 13 293 664 35 447 1.53e-25 110
MS.gene033387.t1 MTR_6g047750 32.222 270 162 10 405 662 401 661 1.88e-25 112
MS.gene033387.t1 MTR_4g026485 25.526 380 246 11 294 662 270 623 9.56e-25 109
MS.gene033387.t1 MTR_0092s0100 24.814 403 277 9 266 662 345 727 2.65e-23 105
MS.gene033387.t1 MTR_0092s0100 24.814 403 277 9 266 662 348 730 3.04e-23 105
MS.gene033387.t1 MTR_4g095500 22.673 419 269 13 293 664 59 469 4.91e-19 90.9
MS.gene033387.t1 MTR_8g077940 27.577 359 218 14 319 662 209 540 3.86e-17 85.5
MS.gene033387.t1 MTR_8g020840 23.786 412 237 15 293 662 171 547 1.67e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033387.t1 AT1G63100 55.635 701 226 23 1 663 1 654 0.0 685
MS.gene033387.t1 AT1G63100 53.638 701 215 21 1 663 1 629 0.0 655
MS.gene033387.t1 AT1G66350 34.961 389 218 11 282 662 144 505 6.06e-67 229
MS.gene033387.t1 AT2G01570 35.990 389 220 11 282 662 213 580 2.25e-65 226
MS.gene033387.t1 AT1G50420 32.360 445 231 10 279 662 43 478 5.32e-65 223
MS.gene033387.t1 AT1G14920 35.219 389 223 11 282 662 161 528 1.27e-64 223
MS.gene033387.t1 AT3G54220 34.949 392 238 8 273 664 277 651 2.04e-64 225
MS.gene033387.t1 AT3G03450 33.162 389 236 11 282 662 172 544 1.46e-61 215
MS.gene033387.t1 AT5G17490 33.073 384 233 11 284 662 151 515 8.33e-60 210
MS.gene033387.t1 AT5G41920 35.641 390 228 13 278 662 28 399 6.06e-59 204
MS.gene033387.t1 AT1G55580 35.036 411 222 12 290 662 41 444 3.72e-53 189
MS.gene033387.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.25e-48 178
MS.gene033387.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.25e-48 178
MS.gene033387.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.25e-48 178
MS.gene033387.t1 AT5G48150 32.225 391 229 13 289 662 118 489 1.25e-48 178
MS.gene033387.t1 AT5G66770 32.172 373 217 12 302 662 235 583 1.86e-48 179
MS.gene033387.t1 AT2G04890 32.540 378 237 12 289 662 49 412 4.90e-48 174
MS.gene033387.t1 AT1G50600 31.552 393 242 12 285 662 145 525 8.76e-48 176
MS.gene033387.t1 AT1G50600 31.980 394 239 13 285 662 216 596 2.89e-47 176
MS.gene033387.t1 AT1G50600 31.980 394 239 13 285 662 220 600 3.47e-47 176
MS.gene033387.t1 AT4G17230 32.041 387 240 10 288 664 153 526 2.22e-46 172
MS.gene033387.t1 AT4G17230 32.041 387 240 10 288 664 153 526 2.22e-46 172
MS.gene033387.t1 AT1G21450 30.900 411 256 11 268 662 194 592 1.27e-45 172
MS.gene033387.t1 AT2G29065 31.026 390 241 13 292 664 251 629 1.29e-42 164
MS.gene033387.t1 AT3G50650 32.609 368 209 14 313 662 195 541 1.61e-42 162
MS.gene033387.t1 AT2G29060 27.619 420 265 12 258 665 265 657 2.99e-41 160
MS.gene033387.t1 AT2G29060 27.619 420 265 12 258 665 302 694 3.49e-41 160
MS.gene033387.t1 AT1G07530 27.979 386 257 10 289 664 392 766 2.24e-40 158
MS.gene033387.t1 AT1G07520 28.288 403 254 16 282 664 143 530 6.76e-40 154
MS.gene033387.t1 AT1G07520 28.288 403 254 16 282 664 143 530 6.76e-40 154
MS.gene033387.t1 AT5G59450 25.887 479 310 14 220 666 136 601 1.71e-39 154
MS.gene033387.t1 AT1G07520 28.288 403 254 16 282 664 307 694 1.90e-39 155
MS.gene033387.t1 AT1G07520 28.288 403 254 16 282 664 308 695 2.16e-39 155
MS.gene033387.t1 AT4G37650 27.481 393 258 9 292 664 145 530 3.71e-39 152
MS.gene033387.t1 AT4G08250 28.856 402 250 14 279 662 93 476 4.78e-39 151
MS.gene033387.t1 AT3G46600 26.877 413 269 10 261 664 192 580 1.84e-36 145
MS.gene033387.t1 AT3G46600 28.010 382 254 9 292 664 3 372 2.35e-36 140
MS.gene033387.t1 AT3G46600 27.119 413 273 10 268 664 148 548 4.34e-36 143
MS.gene033387.t1 AT2G37650 25.700 393 254 11 289 664 331 702 2.96e-32 133
MS.gene033387.t1 AT2G37650 25.700 393 254 11 289 664 343 714 3.68e-32 133
MS.gene033387.t1 AT5G52510 29.283 321 193 10 367 662 328 639 3.19e-27 117
MS.gene033387.t1 AT3G49950 26.700 397 251 12 293 665 30 410 4.53e-26 111
MS.gene033387.t1 AT4G00150 28.212 358 220 14 312 662 226 553 3.70e-25 110
MS.gene033387.t1 AT3G60630 27.036 307 199 10 369 662 327 621 1.08e-23 106
MS.gene033387.t1 AT2G45160 27.302 315 185 11 369 662 347 638 2.02e-21 99.4
MS.gene033387.t1 AT4G36710 23.650 389 261 12 287 662 119 484 1.96e-18 89.4
MS.gene033387.t1 AT3G13840 24.939 413 250 18 275 662 132 509 5.04e-17 85.1

Find 171 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCATTTCATTGCAAAAT+TGG 0.138280 8.4:+22253283 MS.gene033387:CDS
CCTTTCTCTTTGAATTGTTC+TGG 0.202054 8.4:+22252885 MS.gene033387:CDS
ACTGGTTGTGTTGATGCTAT+TGG 0.221753 8.4:+22253239 MS.gene033387:CDS
GTAACAAAGATCACCAATTT+TGG 0.231391 8.4:+22252993 MS.gene033387:CDS
AGATCAAGGATGAGGAGTTT+TGG 0.247813 8.4:+22252652 MS.gene033387:CDS
AGTTTCAGTTTGGCACAATT+TGG 0.253074 8.4:+22252801 MS.gene033387:CDS
TTGGAGAGGCACCAGAGTTT+TGG 0.253860 8.4:+22254124 MS.gene033387:CDS
CTCTCTACACAGTATCTTCT+TGG 0.270369 8.4:+22254320 MS.gene033387:CDS
TGTCACCAACTCACAGATTA+AGG 0.272810 8.4:+22252388 MS.gene033387:CDS
AACCATTTCATTGCAAAATT+GGG 0.272931 8.4:+22253284 MS.gene033387:CDS
CTGCTGTTGTTCTTGACTTC+AGG 0.279004 8.4:-22252561 None:intergenic
CTGGTTGTGTTGATGCTATT+GGG 0.293519 8.4:+22253240 MS.gene033387:CDS
AAATAGAGCTAAGAGGAAAA+AGG 0.307901 8.4:+22252737 MS.gene033387:CDS
ATAAGCACAAATTCGGCTTA+TGG 0.317498 8.4:-22253333 None:intergenic
GCTTGCAGAACATGGATCTT+TGG 0.333074 8.4:+22252701 MS.gene033387:CDS
CAATCCTACAATTGTTGTTA+TGG 0.340164 8.4:+22253910 MS.gene033387:CDS
TGGACACCGGTTGACGTTGC+TGG 0.348429 8.4:+22254340 MS.gene033387:CDS
CATGAAGAGGGTAATGGTTC+GGG 0.351755 8.4:+22253125 MS.gene033387:CDS
TGCCTTTGTCTAACAATCCT+TGG 0.358700 8.4:+22252958 MS.gene033387:CDS
AATACATCTTCAACAGAAAC+TGG 0.362646 8.4:-22254222 None:intergenic
TCAGTTCCTTCATGCTGATA+AGG 0.365523 8.4:-22253152 None:intergenic
CGATGTGAAATGAAGAAACT+TGG 0.373450 8.4:-22253501 None:intergenic
TCTGCCATAACAACAATTGT+AGG 0.376086 8.4:-22253914 None:intergenic
GTGGCTACCCTCACCAAAAT+TGG 0.379531 8.4:-22253006 None:intergenic
AATTTGGAGCTGGAAACTTT+TGG 0.382164 8.4:+22252817 MS.gene033387:CDS
TGAAAGGGAAAGTTTCAGTT+TGG 0.386013 8.4:+22252791 MS.gene033387:CDS
AATCTCTGTGTGCTCATTGA+TGG 0.390998 8.4:-22252453 None:intergenic
CGGTAAAATAAGCACAAATT+CGG 0.394214 8.4:-22253340 None:intergenic
GGAGCAACAGCACTTACTTT+GGG 0.396486 8.4:+22254283 MS.gene033387:CDS
GATGCATGGGAGTCACTGAT+AGG 0.410356 8.4:+22254185 MS.gene033387:CDS
ATCAATGAGCACACAGAGAT+TGG 0.410565 8.4:+22252455 MS.gene033387:CDS
GACTTGAAGATGTAAGGCTT+TGG 0.411643 8.4:+22253768 MS.gene033387:CDS
TGTTCCAAAGTTTAGCTTCA+AGG 0.419556 8.4:+22253612 MS.gene033387:CDS
CCATCAGAGTCACTAGGCTT+TGG 0.420891 8.4:+22253372 MS.gene033387:CDS
AGCAACAACTGCTCAGACAT+TGG 0.422074 8.4:+22252626 MS.gene033387:CDS
TCTTCAAGTCTGTCGACTAC+TGG 0.429762 8.4:-22253755 None:intergenic
TCGAACATGGCTTGGAGGAT+TGG 0.430304 8.4:-22253636 None:intergenic
ACATGAAGAGGGTAATGGTT+CGG 0.431411 8.4:+22253124 MS.gene033387:CDS
TTCGCAGCAGCCGTTGAGGC+CGG 0.436828 8.4:+22252518 MS.gene033387:CDS
AGGAGCAACAGCACTTACTT+TGG 0.437760 8.4:+22254282 MS.gene033387:CDS
AGAGTCAGAGTTTGAGCCTA+AGG 0.440522 8.4:+22253081 MS.gene033387:CDS
GACTCTGATGGCTAAGGCTT+CGG 0.442125 8.4:-22253360 None:intergenic
AAATGAAGAAACTTGGGAAT+TGG 0.443639 8.4:-22253494 None:intergenic
GCAACAACTGCTCAGACATT+GGG 0.444891 8.4:+22252627 MS.gene033387:CDS
AGATGTTAGCTAGGGAGATA+AGG 0.446179 8.4:+22254071 MS.gene033387:CDS
AAGCAAGAGCTGAATGAAAC+CGG 0.462075 8.4:+22253680 MS.gene033387:CDS
TGGCCCTTGAAGCTAAACTT+TGG 0.467104 8.4:-22253616 None:intergenic
CCAGAACAATTCAAAGAGAA+AGG 0.469406 8.4:-22252885 None:intergenic
GAGCTTCCAGCAACGTCAAC+CGG 0.470552 8.4:-22254346 None:intergenic
GTTCTCTGCAGCAGAACATT+CGG 0.471882 8.4:+22252592 MS.gene033387:CDS
TTGTTCTGGAGATGAGGAAA+GGG 0.476638 8.4:+22252899 MS.gene033387:CDS
GAAACCGGAGACAGACTTGC+TGG 0.481646 8.4:+22253695 MS.gene033387:CDS
CATAACAACAATTGTAGGAT+TGG 0.483005 8.4:-22253909 None:intergenic
AGATAGAGGAGATGTTAGCT+AGG 0.487005 8.4:+22254062 MS.gene033387:CDS
CCAAGCCATGTTCGAATCAC+GGG 0.489720 8.4:+22253644 MS.gene033387:CDS
TGATCACAGTGGCCTTCCAC+TGG 0.491665 8.4:+22254021 MS.gene033387:CDS
TCAGACATTGGGAGAGATCA+AGG 0.493655 8.4:+22252638 MS.gene033387:CDS
CCATGTTCGAATCACGGGTG+TGG 0.493749 8.4:+22253649 MS.gene033387:CDS
TCCGATGAGGATCAAGATAG+AGG 0.495969 8.4:+22254048 MS.gene033387:CDS
TGCATTGAGGCTTCTAAACC+AGG 0.497162 8.4:+22253466 MS.gene033387:CDS
CACAGTATCTTCTTGGACAC+CGG 0.497435 8.4:+22254327 MS.gene033387:CDS
CATGTTCGAATCACGGGTGT+GGG 0.498098 8.4:+22253650 MS.gene033387:CDS
TCGACAGACTTGAAGATGTA+AGG 0.499051 8.4:+22253762 MS.gene033387:CDS
ATGCATTCTCATTCAACCTT+AGG 0.503609 8.4:-22253097 None:intergenic
CGGCTTATGGAAGTTCCCCT+TGG 0.503709 8.4:-22253320 None:intergenic
GTTCCAAAGTTTAGCTTCAA+GGG 0.504136 8.4:+22253613 MS.gene033387:CDS
GAAGGAACTGAAGTGGAGAC+AGG 0.505317 8.4:+22253164 MS.gene033387:CDS
ATCGAATGAGATGCTGTTGA+GGG 0.506115 8.4:+22253520 MS.gene033387:CDS
CCCGTGATTCGAACATGGCT+TGG 0.506282 8.4:-22253644 None:intergenic
TTGAAGAGGCTTGCAGAACA+TGG 0.509151 8.4:+22252693 MS.gene033387:CDS
ACCATTTCATTGCAAAATTG+GGG 0.509402 8.4:+22253285 MS.gene033387:CDS
TTCTCCAATTCCCAAAACTC+TGG 0.510256 8.4:-22254135 None:intergenic
CAACAGCACTTACTTTGGGT+TGG 0.512066 8.4:+22254287 MS.gene033387:CDS
TCCTCTATCTTGATCCTCAT+CGG 0.513315 8.4:-22254049 None:intergenic
CATCGAATGAGATGCTGTTG+AGG 0.514579 8.4:+22253519 MS.gene033387:CDS
AGTTTGGCACAATTTGGAGC+TGG 0.514639 8.4:+22252807 MS.gene033387:CDS
GTCTGAGCAGTTGTTGCTAA+AGG 0.515399 8.4:-22252621 None:intergenic
GGTGCAGAAATCACTTCAGC+AGG 0.516593 8.4:-22252933 None:intergenic
GCAGCAGCCGTTGAGGCCGG+TGG 0.517483 8.4:+22252521 MS.gene033387:CDS
GCATGGAGGGTTCAGATGCA+TGG 0.518052 8.4:+22254171 MS.gene033387:CDS
CATGATGATGTTGATGTTGG+TGG 0.518083 8.4:-22253025 None:intergenic
ACTTGGGAATTGGTGTAACC+TGG 0.522388 8.4:-22253484 None:intergenic
AGGAGAAGAGGACGAAGAAG+AGG 0.523774 8.4:+22253184 MS.gene033387:CDS
TTTGAATTGTTCTGGAGATG+AGG 0.524360 8.4:+22252893 MS.gene033387:CDS
CAGTGTTCACAAGCAGGAGC+AGG 0.525223 8.4:+22254258 MS.gene033387:CDS
TTGCTTGCGAAGGAAGGGAC+AGG 0.525446 8.4:+22254101 MS.gene033387:CDS
CAACATCATCATGTCAATGA+AGG 0.526407 8.4:+22253035 MS.gene033387:CDS
ATTTACCTTGTAGCTTCCCT+CGG 0.526979 8.4:+22252478 MS.gene033387:CDS
AGGGTGGGAGGAGTTTGAAG+AGG 0.527118 8.4:+22252679 MS.gene033387:CDS
GACTACTGGATGAAAATCAA+AGG 0.528709 8.4:-22253741 None:intergenic
CAGCAGCCGTTGAGGCCGGT+GGG 0.533021 8.4:+22252522 MS.gene033387:CDS
GTTACAGCAGATTCAAGCCA+AGG 0.534387 8.4:-22252975 None:intergenic
ACATTGTTGCTTGCGAAGGA+AGG 0.535871 8.4:+22254095 MS.gene033387:CDS
CTGACTCTCTGATGAACCAT+TGG 0.536463 8.4:-22253066 None:intergenic
AGGAACATTGTTGCTTGCGA+AGG 0.538101 8.4:+22254091 MS.gene033387:CDS
TCCAAGCCATGTTCGAATCA+CGG 0.544215 8.4:+22253643 MS.gene033387:CDS
CATCAACACAACCAGTAAGC+AGG 0.548431 8.4:-22253232 None:intergenic
GTGATTCGAACATGGCTTGG+AGG 0.550236 8.4:-22253641 None:intergenic
TAATTACAGTGTTCACAAGC+AGG 0.552347 8.4:+22254252 MS.gene033387:CDS
TCACTCCTTAATCTGTGAGT+TGG 0.553015 8.4:-22252393 None:intergenic
AAAACCAGCAAGTCTGTCTC+CGG 0.554388 8.4:-22253699 None:intergenic
CATTGTTGCTTGCGAAGGAA+GGG 0.554638 8.4:+22254096 MS.gene033387:CDS
ATTGTTCTGGAGATGAGGAA+AGG 0.554859 8.4:+22252898 MS.gene033387:CDS
CATGGAGGGTTCAGATGCAT+GGG 0.555361 8.4:+22254172 MS.gene033387:CDS
AGAAAGATGATAGTGGAGCA+TGG 0.555422 8.4:+22254154 MS.gene033387:CDS
GGAACACAATGAGGCGAGGT+TGG 0.556078 8.4:+22253946 MS.gene033387:CDS
GCTTTGGATGCTTCATGTGA+AGG 0.557282 8.4:+22253784 MS.gene033387:CDS
GATAGAGGAGATGTTAGCTA+GGG 0.557387 8.4:+22254063 MS.gene033387:CDS
AAAGGGAAGGTTCAATGCCT+CGG 0.557666 8.4:-22253723 None:intergenic
ATTGGGAGAGATCAAGGATG+AGG 0.561558 8.4:+22252644 MS.gene033387:CDS
TCAGAACATGAAGAGGGTAA+TGG 0.563710 8.4:+22253119 MS.gene033387:CDS
TCATCGGACTCTCCAGTGGA+AGG 0.563830 8.4:-22254033 None:intergenic
GCTAAACTTTGGAACAAGCT+AGG 0.565024 8.4:-22253605 None:intergenic
AAACCATAGACGATGTTGGC+TGG 0.565074 8.4:+22252345 None:intergenic
CCTTAGCCATCAGAGTCACT+AGG 0.567717 8.4:+22253366 MS.gene033387:CDS
GAATTGGAGAAAGATGATAG+TGG 0.568946 8.4:+22254147 MS.gene033387:CDS
TGACATGATGATGTTGATGT+TGG 0.569622 8.4:-22253028 None:intergenic
ACTACTGGATGAAAATCAAA+GGG 0.570042 8.4:-22253740 None:intergenic
CCACACCCGTGATTCGAACA+TGG 0.572154 8.4:-22253649 None:intergenic
TGAAGTGGAGACAGGAGAAG+AGG 0.572761 8.4:+22253172 MS.gene033387:CDS
CGCGAAGAACCGTCTTTCCG+AGG 0.572785 8.4:-22252495 None:intergenic
TGTAGCTTCCCTCGGAAAGA+CGG 0.573619 8.4:+22252486 MS.gene033387:CDS
CCTAGTGACTCTGATGGCTA+AGG 0.575424 8.4:-22253366 None:intergenic
AGATGATAGTGGAGCATGGA+GGG 0.575789 8.4:+22254158 MS.gene033387:CDS
GGCAATGAATGAATTCAAGC+TGG 0.576427 8.4:-22254385 None:intergenic
TCTTTCCGAGGGAAGCTACA+AGG 0.578020 8.4:-22252483 None:intergenic
AAGACACTTCATGATGGTAG+TGG 0.578818 8.4:+22253848 MS.gene033387:CDS
GATGTGAAATGAAGAAACTT+GGG 0.580703 8.4:-22253500 None:intergenic
TCAGCATGAAGGAACTGAAG+TGG 0.582854 8.4:+22253157 MS.gene033387:CDS
TAGGATTGGTGCTACGAATG+AGG 0.584060 8.4:-22253895 None:intergenic
GATATCCCCACCGGCCTCAA+CGG 0.585808 8.4:-22252528 None:intergenic
ATCCTCATCGGACTCTCCAG+TGG 0.587086 8.4:-22254037 None:intergenic
ATGCATGGGAGTCACTGATA+GGG 0.590517 8.4:+22254186 MS.gene033387:CDS
AGCCAAGGATTGTTAGACAA+AGG 0.592331 8.4:-22252960 None:intergenic
AGCAGCCGTTGAGGCCGGTG+GGG 0.594224 8.4:+22252523 MS.gene033387:CDS
CCATTTCATTGCAAAATTGG+GGG 0.595375 8.4:+22253286 MS.gene033387:CDS
TGTTCGAATCACGGGTGTGG+GGG 0.596570 8.4:+22253652 MS.gene033387:CDS
ATGTTCGAATCACGGGTGTG+GGG 0.598829 8.4:+22253651 MS.gene033387:CDS
CCAAAGCCTAGTGACTCTGA+TGG 0.602086 8.4:-22253372 None:intergenic
GCAAGAAGCGGAACACAATG+AGG 0.609256 8.4:+22253937 MS.gene033387:CDS
CTGGATGAAAATCAAAGGGA+AGG 0.609858 8.4:-22253736 None:intergenic
CGCGTTCGCAGCAGCCGTTG+AGG 0.611606 8.4:+22252514 MS.gene033387:CDS
GTGTTGATGCTATTGGGTCA+AGG 0.614329 8.4:+22253246 MS.gene033387:CDS
GGGAATCCTTATCAGCATGA+AGG 0.614596 8.4:+22253146 MS.gene033387:CDS
TTGCGAAGGAAGGGACAGGT+TGG 0.618359 8.4:+22254105 MS.gene033387:CDS
GAATGCATCAGAACATGAAG+AGG 0.618408 8.4:+22253112 MS.gene033387:CDS
AATGAAGGTTCAGTCTCCAA+TGG 0.621452 8.4:+22253050 MS.gene033387:CDS
ACACTTCATGATGGTAGTGG+AGG 0.624635 8.4:+22253851 MS.gene033387:CDS
TGCTATTGGGTCAAGGAATG+TGG 0.631345 8.4:+22253253 MS.gene033387:CDS
CAAGAAGATACTGTGTAGAG+AGG 0.631657 8.4:-22254319 None:intergenic
ATGAAACTGTCACTGCATTG+AGG 0.631836 8.4:+22253453 MS.gene033387:CDS
GCGAAGAACCGTCTTTCCGA+GGG 0.631984 8.4:-22252494 None:intergenic
AAGGAAGGGACAGGTTGGAG+AGG 0.635039 8.4:+22254110 MS.gene033387:CDS
TTCTCCACTGATATCCCCAC+CGG 0.635563 8.4:-22252537 None:intergenic
ATCGACTTCGACATAAAGCA+AGG 0.635920 8.4:+22253572 MS.gene033387:CDS
AAGATGATAGTGGAGCATGG+AGG 0.636506 8.4:+22254157 MS.gene033387:CDS
GTTCACAAGCAGGAGCAGGA+AGG 0.645596 8.4:+22254262 MS.gene033387:CDS
AATGCATCAGAACATGAAGA+GGG 0.650132 8.4:+22253113 MS.gene033387:CDS
CTTCACAAGACACTTCATGA+TGG 0.664343 8.4:+22253842 MS.gene033387:CDS
TGAGCAGTTGTTGCTAAAGG+CGG 0.665701 8.4:-22252618 None:intergenic
GGATTGTTAGACAAAGGCAA+AGG 0.667360 8.4:-22252954 None:intergenic
ATGAAGAGGGTAATGGTTCG+GGG 0.669794 8.4:+22253126 MS.gene033387:CDS
TGTTATGGCAGAGCAAGAAG+CGG 0.671377 8.4:+22253925 MS.gene033387:CDS
ACATAAAGCAAGGACTACAA+TGG 0.673543 8.4:+22253582 MS.gene033387:CDS
GGGGATCTTGCATCTCCAAG+GGG 0.674372 8.4:+22253305 MS.gene033387:CDS
AAGCGGAACACAATGAGGCG+AGG 0.682806 8.4:+22253942 MS.gene033387:CDS
GAGGCCGGTGGGGATATCAG+TGG 0.691692 8.4:+22252533 MS.gene033387:CDS
TTCCACTGGAGAGTCCGATG+AGG 0.697132 8.4:+22254035 MS.gene033387:CDS
TTTGATTCGATTGATCACAG+TGG 0.727954 8.4:+22254010 MS.gene033387:CDS
GACGAAGAAGAGGAGCATCG+CGG 0.735685 8.4:+22253194 MS.gene033387:CDS
TGTGAAGGAACATGAAACTG+TGG 0.739109 8.4:+22253799 MS.gene033387:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAATTTTGATGAATTTGAA+AGG + chr8.4:22252775-22252794 MS.gene033387:CDS 20.0%
!!! GAATTTTGATGAATTTGAAA+GGG + chr8.4:22252776-22252795 MS.gene033387:CDS 20.0%
! AACCATTTCATTGCAAAATT+GGG + chr8.4:22253284-22253303 MS.gene033387:CDS 25.0%
AAATAGAGCTAAGAGGAAAA+AGG + chr8.4:22252737-22252756 MS.gene033387:CDS 30.0%
AAATGAAGAAACTTGGGAAT+TGG - chr8.4:22253497-22253516 None:intergenic 30.0%
AATACATCTTCAACAGAAAC+TGG - chr8.4:22254225-22254244 None:intergenic 30.0%
AATAGAGCTAAGAGGAAAAA+GGG + chr8.4:22252738-22252757 MS.gene033387:CDS 30.0%
ACCATTTCATTGCAAAATTG+GGG + chr8.4:22253285-22253304 MS.gene033387:CDS 30.0%
ACTACTGGATGAAAATCAAA+GGG - chr8.4:22253743-22253762 None:intergenic 30.0%
CAACCATTTCATTGCAAAAT+TGG + chr8.4:22253283-22253302 MS.gene033387:CDS 30.0%
CATAACAACAATTGTAGGAT+TGG - chr8.4:22253912-22253931 None:intergenic 30.0%
CGGTAAAATAAGCACAAATT+CGG - chr8.4:22253343-22253362 None:intergenic 30.0%
GATGTGAAATGAAGAAACTT+GGG - chr8.4:22253503-22253522 None:intergenic 30.0%
! CAATCCTACAATTGTTGTTA+TGG + chr8.4:22253910-22253929 MS.gene033387:CDS 30.0%
! GTAACAAAGATCACCAATTT+TGG + chr8.4:22252993-22253012 MS.gene033387:CDS 30.0%
AATGCATCAGAACATGAAGA+GGG + chr8.4:22253113-22253132 MS.gene033387:CDS 35.0%
ACATAAAGCAAGGACTACAA+TGG + chr8.4:22253582-22253601 MS.gene033387:CDS 35.0%
AGTTTCAGTTTGGCACAATT+TGG + chr8.4:22252801-22252820 MS.gene033387:CDS 35.0%
ATAAGCACAAATTCGGCTTA+TGG - chr8.4:22253336-22253355 None:intergenic 35.0%
ATGCATTCTCATTCAACCTT+AGG - chr8.4:22253100-22253119 None:intergenic 35.0%
CAACATCATCATGTCAATGA+AGG + chr8.4:22253035-22253054 MS.gene033387:CDS 35.0%
CCAGAACAATTCAAAGAGAA+AGG - chr8.4:22252888-22252907 None:intergenic 35.0%
CCATTTCATTGCAAAATTGG+GGG + chr8.4:22253286-22253305 MS.gene033387:CDS 35.0%
CCTTTCTCTTTGAATTGTTC+TGG + chr8.4:22252885-22252904 MS.gene033387:CDS 35.0%
CGATGTGAAATGAAGAAACT+TGG - chr8.4:22253504-22253523 None:intergenic 35.0%
GAATTGGAGAAAGATGATAG+TGG + chr8.4:22254147-22254166 MS.gene033387:CDS 35.0%
GACTACTGGATGAAAATCAA+AGG - chr8.4:22253744-22253763 None:intergenic 35.0%
TAATTACAGTGTTCACAAGC+AGG + chr8.4:22254252-22254271 MS.gene033387:CDS 35.0%
TCTGCCATAACAACAATTGT+AGG - chr8.4:22253917-22253936 None:intergenic 35.0%
TGAAAGGGAAAGTTTCAGTT+TGG + chr8.4:22252791-22252810 MS.gene033387:CDS 35.0%
! CTTTTGCAAATAGAGCTAAG+AGG + chr8.4:22252730-22252749 MS.gene033387:CDS 35.0%
! GTTCCAAAGTTTAGCTTCAA+GGG + chr8.4:22253613-22253632 MS.gene033387:CDS 35.0%
! TGACATGATGATGTTGATGT+TGG - chr8.4:22253031-22253050 None:intergenic 35.0%
! TGTTCCAAAGTTTAGCTTCA+AGG + chr8.4:22253612-22253631 MS.gene033387:CDS 35.0%
! TTTGATTCGATTGATCACAG+TGG + chr8.4:22254010-22254029 MS.gene033387:CDS 35.0%
! TTTTGGTCTGTTCACAGTTT+TGG + chr8.4:22252834-22252853 MS.gene033387:CDS 35.0%
!! AAAGATCACCAATTTTGGTG+AGG + chr8.4:22252998-22253017 MS.gene033387:CDS 35.0%
!! AAGATCACCAATTTTGGTGA+GGG + chr8.4:22252999-22253018 MS.gene033387:CDS 35.0%
!! AATTTGGAGCTGGAAACTTT+TGG + chr8.4:22252817-22252836 MS.gene033387:CDS 35.0%
!! TACTTCTCGTGATTTTGACA+GGG + chr8.4:22253418-22253437 MS.gene033387:CDS 35.0%
!! TTTGAATTGTTCTGGAGATG+AGG + chr8.4:22252893-22252912 MS.gene033387:CDS 35.0%
AAGACACTTCATGATGGTAG+TGG + chr8.4:22253848-22253867 MS.gene033387:CDS 40.0%
AAGCAAGAGCTGAATGAAAC+CGG + chr8.4:22253680-22253699 MS.gene033387:CDS 40.0%
AATCTCTGTGTGCTCATTGA+TGG - chr8.4:22252456-22252475 None:intergenic 40.0%
AATGAAGGTTCAGTCTCCAA+TGG + chr8.4:22253050-22253069 MS.gene033387:CDS 40.0%
AGATAGAGGAGATGTTAGCT+AGG + chr8.4:22254062-22254081 MS.gene033387:CDS 40.0%
AGATGTTAGCTAGGGAGATA+AGG + chr8.4:22254071-22254090 MS.gene033387:CDS 40.0%
AGCCAAGGATTGTTAGACAA+AGG - chr8.4:22252963-22252982 None:intergenic 40.0%
ATCAATGAGCACACAGAGAT+TGG + chr8.4:22252455-22252474 MS.gene033387:CDS 40.0%
ATCGAATGAGATGCTGTTGA+GGG + chr8.4:22253520-22253539 MS.gene033387:CDS 40.0%
ATGAAACTGTCACTGCATTG+AGG + chr8.4:22253453-22253472 MS.gene033387:CDS 40.0%
ATTTACCTTGTAGCTTCCCT+CGG + chr8.4:22252478-22252497 MS.gene033387:CDS 40.0%
CAAGAAGATACTGTGTAGAG+AGG - chr8.4:22254322-22254341 None:intergenic 40.0%
CTCTCTACACAGTATCTTCT+TGG + chr8.4:22254320-22254339 MS.gene033387:CDS 40.0%
CTGGATGAAAATCAAAGGGA+AGG - chr8.4:22253739-22253758 None:intergenic 40.0%
CTTCACAAGACACTTCATGA+TGG + chr8.4:22253842-22253861 MS.gene033387:CDS 40.0%
GAATGCATCAGAACATGAAG+AGG + chr8.4:22253112-22253131 MS.gene033387:CDS 40.0%
GACTTGAAGATGTAAGGCTT+TGG + chr8.4:22253768-22253787 MS.gene033387:CDS 40.0%
GATAGAGGAGATGTTAGCTA+GGG + chr8.4:22254063-22254082 MS.gene033387:CDS 40.0%
GCTAAGAGGAAAAAGGGTAA+TGG + chr8.4:22252744-22252763 MS.gene033387:CDS 40.0%
GGATTGTTAGACAAAGGCAA+AGG - chr8.4:22252957-22252976 None:intergenic 40.0%
TCACTCCTTAATCTGTGAGT+TGG - chr8.4:22252396-22252415 None:intergenic 40.0%
TCAGAACATGAAGAGGGTAA+TGG + chr8.4:22253119-22253138 MS.gene033387:CDS 40.0%
TCAGTTCCTTCATGCTGATA+AGG - chr8.4:22253155-22253174 None:intergenic 40.0%
TCCTCTATCTTGATCCTCAT+CGG - chr8.4:22254052-22254071 None:intergenic 40.0%
TCGACAGACTTGAAGATGTA+AGG + chr8.4:22253762-22253781 MS.gene033387:CDS 40.0%
TGCCTTTGTCTAACAATCCT+TGG + chr8.4:22252958-22252977 MS.gene033387:CDS 40.0%
TGTCACCAACTCACAGATTA+AGG + chr8.4:22252388-22252407 MS.gene033387:CDS 40.0%
TGTGAAGGAACATGAAACTG+TGG + chr8.4:22253799-22253818 MS.gene033387:CDS 40.0%
TTCTCCAATTCCCAAAACTC+TGG - chr8.4:22254138-22254157 None:intergenic 40.0%
! AAGAGGAAAAAGGGTAATGG+TGG + chr8.4:22252747-22252766 MS.gene033387:CDS 40.0%
! ACATGAAGAGGGTAATGGTT+CGG + chr8.4:22253124-22253143 MS.gene033387:CDS 40.0%
! ACTGGTTGTGTTGATGCTAT+TGG + chr8.4:22253239-22253258 MS.gene033387:CDS 40.0%
! AGATCAAGGATGAGGAGTTT+TGG + chr8.4:22252652-22252671 MS.gene033387:CDS 40.0%
! ATCGACTTCGACATAAAGCA+AGG + chr8.4:22253572-22253591 MS.gene033387:CDS 40.0%
! CATGATGATGTTGATGTTGG+TGG - chr8.4:22253028-22253047 None:intergenic 40.0%
! CCCCCAATTTTGCAATGAAA+TGG - chr8.4:22253289-22253308 None:intergenic 40.0%
! CTGGTTGTGTTGATGCTATT+GGG + chr8.4:22253240-22253259 MS.gene033387:CDS 40.0%
! GATCAAGGATGAGGAGTTTT+GGG + chr8.4:22252653-22252672 MS.gene033387:CDS 40.0%
!! AGAAAGATGATAGTGGAGCA+TGG + chr8.4:22254154-22254173 MS.gene033387:CDS 40.0%
!! ATTGTTCTGGAGATGAGGAA+AGG + chr8.4:22252898-22252917 MS.gene033387:CDS 40.0%
!! CTACTTCTCGTGATTTTGAC+AGG + chr8.4:22253417-22253436 MS.gene033387:CDS 40.0%
!! GCGCTGATTTGAAAAACATG+AGG - chr8.4:22253398-22253417 None:intergenic 40.0%
!! GCTAAACTTTGGAACAAGCT+AGG - chr8.4:22253608-22253627 None:intergenic 40.0%
!! GGCTTTTGAAGGGAAAGAAA+GGG + chr8.4:22253541-22253560 MS.gene033387:CDS 40.0%
!! TTGTTCTGGAGATGAGGAAA+GGG + chr8.4:22252899-22252918 MS.gene033387:CDS 40.0%
!!! AGGAGCATTGAGAGACTTTT+TGG + chr8.4:22253871-22253890 MS.gene033387:CDS 40.0%
!!! GGAGCATTGAGAGACTTTTT+GGG + chr8.4:22253872-22253891 MS.gene033387:CDS 40.0%
AAAACCAGCAAGTCTGTCTC+CGG - chr8.4:22253702-22253721 None:intergenic 45.0%
AAAGGGAAGGTTCAATGCCT+CGG - chr8.4:22253726-22253745 None:intergenic 45.0%
ACTTGGGAATTGGTGTAACC+TGG - chr8.4:22253487-22253506 None:intergenic 45.0%
AGAGTCAGAGTTTGAGCCTA+AGG + chr8.4:22253081-22253100 MS.gene033387:CDS 45.0%
AGCAACAACTGCTCAGACAT+TGG + chr8.4:22252626-22252645 MS.gene033387:CDS 45.0%
AGGAGCAACAGCACTTACTT+TGG + chr8.4:22254282-22254301 MS.gene033387:CDS 45.0%
ATGCATGGGAGTCACTGATA+GGG + chr8.4:22254186-22254205 MS.gene033387:CDS 45.0%
ATTGGGAGAGATCAAGGATG+AGG + chr8.4:22252644-22252663 MS.gene033387:CDS 45.0%
CAACAGCACTTACTTTGGGT+TGG + chr8.4:22254287-22254306 MS.gene033387:CDS 45.0%
CACAGTATCTTCTTGGACAC+CGG + chr8.4:22254327-22254346 MS.gene033387:CDS 45.0%
CATCAACACAACCAGTAAGC+AGG - chr8.4:22253235-22253254 None:intergenic 45.0%
CATCGAATGAGATGCTGTTG+AGG + chr8.4:22253519-22253538 MS.gene033387:CDS 45.0%
CTGACTCTCTGATGAACCAT+TGG - chr8.4:22253069-22253088 None:intergenic 45.0%
CTGCTGTTGTTCTTGACTTC+AGG - chr8.4:22252564-22252583 None:intergenic 45.0%
GCAACAACTGCTCAGACATT+GGG + chr8.4:22252627-22252646 MS.gene033387:CDS 45.0%
GCTTGCAGAACATGGATCTT+TGG + chr8.4:22252701-22252720 MS.gene033387:CDS 45.0%
GGAGCAACAGCACTTACTTT+GGG + chr8.4:22254283-22254302 MS.gene033387:CDS 45.0%
GGGAATCCTTATCAGCATGA+AGG + chr8.4:22253146-22253165 MS.gene033387:CDS 45.0%
GTCTGAGCAGTTGTTGCTAA+AGG - chr8.4:22252624-22252643 None:intergenic 45.0%
GTTACAGCAGATTCAAGCCA+AGG - chr8.4:22252978-22252997 None:intergenic 45.0%
GTTCTCTGCAGCAGAACATT+CGG + chr8.4:22252592-22252611 MS.gene033387:CDS 45.0%
TCAAGCTGGCTGAGAAAAAG+AGG - chr8.4:22254374-22254393 None:intergenic 45.0%
TCAGACATTGGGAGAGATCA+AGG + chr8.4:22252638-22252657 MS.gene033387:CDS 45.0%
TCAGCATGAAGGAACTGAAG+TGG + chr8.4:22253157-22253176 MS.gene033387:CDS 45.0%
TCCAAGCCATGTTCGAATCA+CGG + chr8.4:22253643-22253662 MS.gene033387:CDS 45.0%
TCCGATGAGGATCAAGATAG+AGG + chr8.4:22254048-22254067 MS.gene033387:CDS 45.0%
TGAGCAGTTGTTGCTAAAGG+CGG - chr8.4:22252621-22252640 None:intergenic 45.0%
TGCATTGAGGCTTCTAAACC+AGG + chr8.4:22253466-22253485 MS.gene033387:CDS 45.0%
TGCTATTGGGTCAAGGAATG+TGG + chr8.4:22253253-22253272 MS.gene033387:CDS 45.0%
TGGCCCTTGAAGCTAAACTT+TGG - chr8.4:22253619-22253638 None:intergenic 45.0%
TGTTATGGCAGAGCAAGAAG+CGG + chr8.4:22253925-22253944 MS.gene033387:CDS 45.0%
TTGAAGAGGCTTGCAGAACA+TGG + chr8.4:22252693-22252712 MS.gene033387:CDS 45.0%
! ACATTGTTGCTTGCGAAGGA+AGG + chr8.4:22254095-22254114 MS.gene033387:CDS 45.0%
! AGGAACATTGTTGCTTGCGA+AGG + chr8.4:22254091-22254110 MS.gene033387:CDS 45.0%
! AGTTTGGCACAATTTGGAGC+TGG + chr8.4:22252807-22252826 MS.gene033387:CDS 45.0%
! ATGAAGAGGGTAATGGTTCG+GGG + chr8.4:22253126-22253145 MS.gene033387:CDS 45.0%
! ATGCTGTTGAGGGCTTTTGA+AGG + chr8.4:22253530-22253549 MS.gene033387:CDS 45.0%
! CATGAAGAGGGTAATGGTTC+GGG + chr8.4:22253125-22253144 MS.gene033387:CDS 45.0%
! CATTGTTGCTTGCGAAGGAA+GGG + chr8.4:22254096-22254115 MS.gene033387:CDS 45.0%
! GCTTTGGATGCTTCATGTGA+AGG + chr8.4:22253784-22253803 MS.gene033387:CDS 45.0%
! GTGTTGATGCTATTGGGTCA+AGG + chr8.4:22253246-22253265 MS.gene033387:CDS 45.0%
! TCTTCAAGTCTGTCGACTAC+TGG - chr8.4:22253758-22253777 None:intergenic 45.0%
! TGCTGTTGAGGGCTTTTGAA+GGG + chr8.4:22253531-22253550 MS.gene033387:CDS 45.0%
!! AAGATGATAGTGGAGCATGG+AGG + chr8.4:22254157-22254176 MS.gene033387:CDS 45.0%
!! ACACTTCATGATGGTAGTGG+AGG + chr8.4:22253851-22253870 MS.gene033387:CDS 45.0%
!! AGATGATAGTGGAGCATGGA+GGG + chr8.4:22254158-22254177 MS.gene033387:CDS 45.0%
!! GGGCTTTTGAAGGGAAAGAA+AGG + chr8.4:22253540-22253559 MS.gene033387:CDS 45.0%
!! TAGGATTGGTGCTACGAATG+AGG - chr8.4:22253898-22253917 None:intergenic 45.0%
!! TGGTCTGTTCACAGTTTTGG+AGG + chr8.4:22252837-22252856 MS.gene033387:CDS 45.0%
!! TTCTCGTGATTTTGACAGGG+TGG + chr8.4:22253421-22253440 MS.gene033387:CDS 45.0%
!!! GATGAGGAGTTTTGGGAGAA+GGG + chr8.4:22252660-22252679 MS.gene033387:CDS 45.0%
!!! GGTCTGTTCACAGTTTTGGA+GGG + chr8.4:22252838-22252857 MS.gene033387:CDS 45.0%
AGGAGAAGAGGACGAAGAAG+AGG + chr8.4:22253184-22253203 MS.gene033387:CDS 50.0%
ATGTTCGAATCACGGGTGTG+GGG + chr8.4:22253651-22253670 MS.gene033387:CDS 50.0%
CATGGAGGGTTCAGATGCAT+GGG + chr8.4:22254172-22254191 MS.gene033387:CDS 50.0%
CATGTTCGAATCACGGGTGT+GGG + chr8.4:22253650-22253669 MS.gene033387:CDS 50.0%
CCAAAGCCTAGTGACTCTGA+TGG - chr8.4:22253375-22253394 None:intergenic 50.0%
CCAAGCCATGTTCGAATCAC+GGG + chr8.4:22253644-22253663 MS.gene033387:CDS 50.0%
CCATCAGAGTCACTAGGCTT+TGG + chr8.4:22253372-22253391 MS.gene033387:CDS 50.0%
CCTAGTGACTCTGATGGCTA+AGG - chr8.4:22253369-22253388 None:intergenic 50.0%
CCTTAGCCATCAGAGTCACT+AGG + chr8.4:22253366-22253385 MS.gene033387:CDS 50.0%
GAAGGAACTGAAGTGGAGAC+AGG + chr8.4:22253164-22253183 MS.gene033387:CDS 50.0%
GACTCTGATGGCTAAGGCTT+CGG - chr8.4:22253363-22253382 None:intergenic 50.0%
GATGCATGGGAGTCACTGAT+AGG + chr8.4:22254185-22254204 MS.gene033387:CDS 50.0%
GCAAGAAGCGGAACACAATG+AGG + chr8.4:22253937-22253956 MS.gene033387:CDS 50.0%
GGTGCAGAAATCACTTCAGC+AGG - chr8.4:22252936-22252955 None:intergenic 50.0%
GTGGCTACCCTCACCAAAAT+TGG - chr8.4:22253009-22253028 None:intergenic 50.0%
TCTTTCCGAGGGAAGCTACA+AGG - chr8.4:22252486-22252505 None:intergenic 50.0%
TGAAGTGGAGACAGGAGAAG+AGG + chr8.4:22253172-22253191 MS.gene033387:CDS 50.0%
TGTAGCTTCCCTCGGAAAGA+CGG + chr8.4:22252486-22252505 MS.gene033387:CDS 50.0%
TTCTCCACTGATATCCCCAC+CGG - chr8.4:22252540-22252559 None:intergenic 50.0%
! GAGCTTTTGAGCCTGCTTAC+TGG + chr8.4:22253221-22253240 MS.gene033387:CDS 50.0%
! GTGATTCGAACATGGCTTGG+AGG - chr8.4:22253644-22253663 None:intergenic 50.0%
! TCGAACATGGCTTGGAGGAT+TGG - chr8.4:22253639-22253658 None:intergenic 50.0%
!! TCGTGATTTTGACAGGGTGG+TGG + chr8.4:22253424-22253443 MS.gene033387:CDS 50.0%
!!! AGGAGTTTTGGGAGAAGGGT+GGG + chr8.4:22252664-22252683 MS.gene033387:CDS 50.0%
!!! GGATGAGGAGTTTTGGGAGA+AGG + chr8.4:22252659-22252678 MS.gene033387:CDS 50.0%
!!! GGCACCAGAGTTTTGGGAAT+TGG + chr8.4:22254131-22254150 MS.gene033387:CDS 50.0%
!!! TGGAGAGGCACCAGAGTTTT+GGG + chr8.4:22254125-22254144 MS.gene033387:CDS 50.0%
!!! TTGGAGAGGCACCAGAGTTT+TGG + chr8.4:22254124-22254143 MS.gene033387:CDS 50.0%
AAGCGGAACACAATGAGGCG+AGG + chr8.4:22253942-22253961 MS.gene033387:CDS 55.0%
AGGGTGGGAGGAGTTTGAAG+AGG + chr8.4:22252679-22252698 MS.gene033387:CDS 55.0%
ATCCTCATCGGACTCTCCAG+TGG - chr8.4:22254040-22254059 None:intergenic 55.0%
CAGTGTTCACAAGCAGGAGC+AGG + chr8.4:22254258-22254277 MS.gene033387:CDS 55.0%
CCACACCCGTGATTCGAACA+TGG - chr8.4:22253652-22253671 None:intergenic 55.0%
CCATGTTCGAATCACGGGTG+TGG + chr8.4:22253649-22253668 MS.gene033387:CDS 55.0%
CCCGTGATTCGAACATGGCT+TGG - chr8.4:22253647-22253666 None:intergenic 55.0%
CGGCTTATGGAAGTTCCCCT+TGG - chr8.4:22253323-22253342 None:intergenic 55.0%
GAAACCGGAGACAGACTTGC+TGG + chr8.4:22253695-22253714 MS.gene033387:CDS 55.0%
GAGCTTCCAGCAACGTCAAC+CGG - chr8.4:22254349-22254368 None:intergenic 55.0%
GCATGGAGGGTTCAGATGCA+TGG + chr8.4:22254171-22254190 MS.gene033387:CDS 55.0%
GCGAAGAACCGTCTTTCCGA+GGG - chr8.4:22252497-22252516 None:intergenic 55.0%
GGAACACAATGAGGCGAGGT+TGG + chr8.4:22253946-22253965 MS.gene033387:CDS 55.0%
GGGGATCTTGCATCTCCAAG+GGG + chr8.4:22253305-22253324 MS.gene033387:CDS 55.0%
GGGGGATCTTGCATCTCCAA+GGG + chr8.4:22253304-22253323 MS.gene033387:CDS 55.0%
GTTCACAAGCAGGAGCAGGA+AGG + chr8.4:22254262-22254281 MS.gene033387:CDS 55.0%
TCATCGGACTCTCCAGTGGA+AGG - chr8.4:22254036-22254055 None:intergenic 55.0%
TGATCACAGTGGCCTTCCAC+TGG + chr8.4:22254021-22254040 MS.gene033387:CDS 55.0%
TGGGGGATCTTGCATCTCCA+AGG + chr8.4:22253303-22253322 MS.gene033387:CDS 55.0%
TGTTCGAATCACGGGTGTGG+GGG + chr8.4:22253652-22253671 MS.gene033387:CDS 55.0%
TTCCACTGGAGAGTCCGATG+AGG + chr8.4:22254035-22254054 MS.gene033387:CDS 55.0%
TTGCGAAGGAAGGGACAGGT+TGG + chr8.4:22254105-22254124 MS.gene033387:CDS 55.0%
TTGCTTGCGAAGGAAGGGAC+AGG + chr8.4:22254101-22254120 MS.gene033387:CDS 55.0%
! AAGGAAGGGACAGGTTGGAG+AGG + chr8.4:22254110-22254129 MS.gene033387:CDS 55.0%
!! GACGAAGAAGAGGAGCATCG+CGG + chr8.4:22253194-22253213 MS.gene033387:CDS 55.0%
!!! AGTTTTGGGAGAAGGGTGGG+AGG + chr8.4:22252667-22252686 MS.gene033387:CDS 55.0%
!!! CAGACTTGCTGGTTTTGCCG+AGG + chr8.4:22253706-22253725 MS.gene033387:CDS 55.0%
!!! GAGGAGTTTTGGGAGAAGGG+TGG + chr8.4:22252663-22252682 MS.gene033387:CDS 55.0%
CGCGAAGAACCGTCTTTCCG+AGG - chr8.4:22252498-22252517 None:intergenic 60.0%
GATATCCCCACCGGCCTCAA+CGG - chr8.4:22252531-22252550 None:intergenic 60.0%
! TGGACACCGGTTGACGTTGC+TGG + chr8.4:22254340-22254359 MS.gene033387:CDS 60.0%
GAGGCCGGTGGGGATATCAG+TGG + chr8.4:22252533-22252552 MS.gene033387:CDS 65.0%
TTCGCAGCAGCCGTTGAGGC+CGG + chr8.4:22252518-22252537 MS.gene033387:CDS 65.0%
AGCAGCCGTTGAGGCCGGTG+GGG + chr8.4:22252523-22252542 MS.gene033387:CDS 70.0%
CAGCAGCCGTTGAGGCCGGT+GGG + chr8.4:22252522-22252541 MS.gene033387:CDS 70.0%
CGCGTTCGCAGCAGCCGTTG+AGG + chr8.4:22252514-22252533 MS.gene033387:CDS 70.0%
GCAGCAGCCGTTGAGGCCGG+TGG + chr8.4:22252521-22252540 MS.gene033387:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 22252357 22254393 22252357 ID=MS.gene033387
chr8.4 mRNA 22252357 22254393 22252357 ID=MS.gene033387.t1;Parent=MS.gene033387
chr8.4 exon 22252357 22254393 22252357 ID=MS.gene033387.t1.exon1;Parent=MS.gene033387.t1
chr8.4 CDS 22252357 22254393 22252357 ID=cds.MS.gene033387.t1;Parent=MS.gene033387.t1
Gene Sequence

>MS.gene033387

ATGTTGGCTGGTTGTTCTAGCTCGACATTGTTGTCACCAACTCACAGATTAAGGAGTGAAGCATCAGCACAGTTTCAAGCTTGTCATTTTCAGCTACCATCAATGAGCACACAGAGATTGGATTTACCTTGTAGCTTCCCTCGGAAAGACGGTTCTTCGCGTTCGCAGCAGCCGTTGAGGCCGGTGGGGATATCAGTGGAGAAGCCTGAAGTCAAGAACAACAGCAGTAGCACTTGTTCTCTGCAGCAGAACATTCGGTTACCGCCTTTAGCAACAACTGCTCAGACATTGGGAGAGATCAAGGATGAGGAGTTTTGGGAGAAGGGTGGGAGGAGTTTGAAGAGGCTTGCAGAACATGGATCTTTGGATGAGTCTTTTGCAAATAGAGCTAAGAGGAAAAAGGGTAATGGTGGTGATGAGAATTTTGATGAATTTGAAAGGGAAAGTTTCAGTTTGGCACAATTTGGAGCTGGAAACTTTTGGTCTGTTCACAGTTTTGGAGGGTTTAATAGTAATAGCTCTTCGATTCCTTTCTCTTTGAATTGTTCTGGAGATGAGGAAAGGGTTTGTTTTGCTCCTGCTGAAGTGATTTCTGCACCTTTGCCTTTGTCTAACAATCCTTGGCTTGAATCTGCTGTAACAAAGATCACCAATTTTGGTGAGGGTAGCCACCAACATCAACATCATCATGTCAATGAAGGTTCAGTCTCCAATGGTTCATCAGAGAGTCAGAGTTTGAGCCTAAGGTTGAATGAGAATGCATCAGAACATGAAGAGGGTAATGGTTCGGGGAATCCTTATCAGCATGAAGGAACTGAAGTGGAGACAGGAGAAGAGGACGAAGAAGAGGAGCATCGCGGTTTTGAGCTTTTGAGCCTGCTTACTGGTTGTGTTGATGCTATTGGGTCAAGGAATGTGGCTGCAGTCAACCATTTCATTGCAAAATTGGGGGATCTTGCATCTCCAAGGGGAACTTCCATAAGCCGAATTTGTGCTTATTTTACCGAAGCCTTAGCCATCAGAGTCACTAGGCTTTGGCCTCATGTTTTTCAAATCAGCGCTACTTCTCGTGATTTTGACAGGGTGGTGGATGATGATGAAACTGTCACTGCATTGAGGCTTCTAAACCAGGTTACACCAATTCCCAAGTTTCTTCATTTCACATCGAATGAGATGCTGTTGAGGGCTTTTGAAGGGAAAGAAAGGGTTCACATTATCGACTTCGACATAAAGCAAGGACTACAATGGCCTAGCTTGTTCCAAAGTTTAGCTTCAAGGGCCAATCCTCCAAGCCATGTTCGAATCACGGGTGTGGGGGAATCGAAGCAAGAGCTGAATGAAACCGGAGACAGACTTGCTGGTTTTGCCGAGGCATTGAACCTTCCCTTTGATTTTCATCCAGTAGTCGACAGACTTGAAGATGTAAGGCTTTGGATGCTTCATGTGAAGGAACATGAAACTGTGGCTGTGAACTGTGTTTTGCAGCTTCACAAGACACTTCATGATGGTAGTGGAGGAGCATTGAGAGACTTTTTGGGCCTCATTCGTAGCACCAATCCTACAATTGTTGTTATGGCAGAGCAAGAAGCGGAACACAATGAGGCGAGGTTGGACTCAAGAGTCTGCAATTCATTGAAATATTACTCAGCGCTGTTTGATTCGATTGATCACAGTGGCCTTCCACTGGAGAGTCCGATGAGGATCAAGATAGAGGAGATGTTAGCTAGGGAGATAAGGAACATTGTTGCTTGCGAAGGAAGGGACAGGTTGGAGAGGCACCAGAGTTTTGGGAATTGGAGAAAGATGATAGTGGAGCATGGAGGGTTCAGATGCATGGGAGTCACTGATAGGGAACTGATTCAGAGCCAGTTTCTGTTGAAGATGTATTCTTGTGATAATTACAGTGTTCACAAGCAGGAGCAGGAAGGAGCAACAGCACTTACTTTGGGTTGGCTTGATCAACCTCTCTACACAGTATCTTCTTGGACACCGGTTGACGTTGCTGGAAGCTCATCCTCTTTTTCTCAGCCAGCTTGA
Protein sequence

>MS.gene033387.t1

MLAGCSSSTLLSPTHRLRSEASAQFQACHFQLPSMSTQRLDLPCSFPRKDGSSRSQQPLRPVGISVEKPEVKNNSSSTCSLQQNIRLPPLATTAQTLGEIKDEEFWEKGGRSLKRLAEHGSLDESFANRAKRKKGNGGDENFDEFERESFSLAQFGAGNFWSVHSFGGFNSNSSSIPFSLNCSGDEERVCFAPAEVISAPLPLSNNPWLESAVTKITNFGEGSHQHQHHHVNEGSVSNGSSESQSLSLRLNENASEHEEGNGSGNPYQHEGTEVETGEEDEEEEHRGFELLSLLTGCVDAIGSRNVAAVNHFIAKLGDLASPRGTSISRICAYFTEALAIRVTRLWPHVFQISATSRDFDRVVDDDETVTALRLLNQVTPIPKFLHFTSNEMLLRAFEGKERVHIIDFDIKQGLQWPSLFQSLASRANPPSHVRITGVGESKQELNETGDRLAGFAEALNLPFDFHPVVDRLEDVRLWMLHVKEHETVAVNCVLQLHKTLHDGSGGALRDFLGLIRSTNPTIVVMAEQEAEHNEARLDSRVCNSLKYYSALFDSIDHSGLPLESPMRIKIEEMLAREIRNIVACEGRDRLERHQSFGNWRKMIVEHGGFRCMGVTDRELIQSQFLLKMYSCDNYSVHKQEQEGATALTLGWLDQPLYTVSSWTPVDVAGSSSSFSQPA