AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033571

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033571.t1 MTR_7g081270 96.127 284 11 0 1 284 1 284 0.0 563
MS.gene033571.t1 MTR_2g049780 42.991 214 118 4 67 279 94 304 3.70e-46 157
MS.gene033571.t1 MTR_2g067230 32.852 277 169 8 6 279 37 299 3.28e-35 129
MS.gene033571.t1 MTR_5g041630 33.708 267 150 8 19 277 43 290 3.13e-31 118
MS.gene033571.t1 MTR_6g003960 26.636 214 110 4 75 283 420 591 5.75e-19 87.0
MS.gene033571.t1 MTR_2g034600 31.553 206 127 7 78 279 348 543 2.37e-16 79.3
MS.gene033571.t1 MTR_2g034600 31.553 206 127 7 78 279 287 482 2.56e-16 79.0
MS.gene033571.t1 MTR_2g034600 31.553 206 127 7 78 279 352 547 2.59e-16 79.0
MS.gene033571.t1 MTR_4g084630 28.571 217 135 5 77 283 116 322 6.92e-15 73.9
MS.gene033571.t1 MTR_5g094610 26.484 219 151 6 72 283 260 475 7.54e-15 74.7
MS.gene033571.t1 MTR_4g070060 27.184 206 143 5 77 279 384 585 9.69e-15 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033571.t1 AT3G18870 55.823 249 106 3 36 284 29 273 8.40e-97 286
MS.gene033571.t1 AT2G03050 36.667 240 146 5 43 279 32 268 1.56e-43 150
MS.gene033571.t1 AT2G03050 36.667 240 146 5 43 279 33 269 1.65e-43 150
MS.gene033571.t1 AT2G34620 39.524 210 123 4 71 279 84 290 3.43e-43 149
MS.gene033571.t1 AT2G36000 34.440 241 146 8 44 279 74 307 4.15e-31 118
MS.gene033571.t1 AT2G36000 34.440 241 146 8 44 279 74 307 4.24e-31 119
MS.gene033571.t1 AT1G78930 27.830 212 116 3 72 283 404 578 5.74e-18 84.0
MS.gene033571.t1 AT5G55580 27.854 219 148 7 72 283 253 468 8.49e-16 77.4
MS.gene033571.t1 AT5G55580 27.854 219 148 7 72 283 147 362 1.24e-15 76.6
MS.gene033571.t1 AT4G14605 30.097 206 130 8 78 279 272 467 5.32e-15 75.1
MS.gene033571.t1 AT4G14605 30.097 206 130 8 78 279 272 467 5.32e-15 75.1
MS.gene033571.t1 AT4G38160 27.570 214 142 6 77 283 117 324 2.87e-14 72.4
MS.gene033571.t1 AT4G38160 27.570 214 142 6 77 283 117 324 2.87e-14 72.4
MS.gene033571.t1 AT2G21710 27.273 242 131 5 78 279 356 592 3.00e-14 73.2
MS.gene033571.t1 AT4G38160 27.570 214 142 6 77 283 117 324 3.46e-14 72.4
MS.gene033571.t1 AT4G38160 27.570 214 142 6 77 283 117 324 4.43e-14 72.0

Find 106 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCAAGGTTGCCGGCGATAT+TGG 0.211818 7.4:+25228396 MS.gene033571:CDS
GCAAGGTTGCCGGCGATATT+GGG 0.271051 7.4:+25228397 MS.gene033571:CDS
TCCTGTTAGTTGGCCTGTTC+AGG 0.273978 7.4:-25228273 None:intergenic
GTGGCCGAAGTTTGTGTATT+TGG 0.292279 7.4:+25228441 MS.gene033571:CDS
GGAGAGGGAGGTAGGGGAAA+TGG 0.315974 7.4:-25228098 None:intergenic
TCAAGGTGAGGTAGGTTGTT+TGG 0.324999 7.4:-25227968 None:intergenic
AAATGGAGGTGGTGAAGAGG+TGG 0.352753 7.4:-25228081 None:intergenic
TTGATGTAACCGTTTCTGTT+AGG 0.373078 7.4:-25227875 None:intergenic
TTTGGCGTTGGTGGAAAGAT+TGG 0.381016 7.4:-25227796 None:intergenic
GAATGTTTGTGCAAGGTTGC+CGG 0.384227 7.4:+25228387 MS.gene033571:CDS
ACCTGAACAGGCCAACTAAC+AGG 0.386362 7.4:+25228272 MS.gene033571:CDS
GGGACAATGGTGGACTAGCC+GGG 0.387237 7.4:-25228059 None:intergenic
GAGGATAAGAAGATGGAGTT+TGG 0.390964 7.4:-25227814 None:intergenic
ACATCGGTGAAGAGGAGGTT+AGG 0.396135 7.4:-25228193 None:intergenic
ATTTGAAAGAAAGAGGTGTT+AGG 0.409873 7.4:+25228557 MS.gene033571:CDS
GGAGGTAGGGGAAATGGAGG+TGG 0.413210 7.4:-25228092 None:intergenic
GAAAGAGATGGAGAGGGAGG+TGG 0.414641 7.4:+25228465 MS.gene033571:CDS
GAGGGAGGTAGGGGAAATGG+AGG 0.418156 7.4:-25228095 None:intergenic
GGGGACAATGGTGGACTAGC+CGG 0.421815 7.4:-25228060 None:intergenic
AAGATGGAGTTTGGCGTTGG+TGG 0.422753 7.4:-25227805 None:intergenic
GAAGGGTTGGTTTGAGGATG+TGG 0.426825 7.4:-25228219 None:intergenic
AGGTTTCTTGAGGAAGTGGT+TGG 0.427900 7.4:+25228343 MS.gene033571:CDS
TCTTTCAAATGCAAATGCCT+TGG 0.434972 7.4:-25228544 None:intergenic
TGAGAGGGAGGATAAGAAGA+TGG 0.437100 7.4:-25227821 None:intergenic
GGAAGTGAAGGGTTGGTTTG+AGG 0.437265 7.4:-25228225 None:intergenic
GGTTAGGGCAACGGAGGATA+AGG 0.439054 7.4:-25228177 None:intergenic
GAGGTGAGGAAGGTGAAGAC+GGG 0.439852 7.4:-25228121 None:intergenic
TTGGAGTTAGTAACCTGAAC+AGG 0.454578 7.4:+25228260 MS.gene033571:CDS
AACCGTTTCTGTTAGGATAA+CGG 0.454941 7.4:-25227868 None:intergenic
CACTTCCTCCGCGAGGAAGT+TGG 0.461687 7.4:+25228241 MS.gene033571:CDS
AATGGAGGTGGTGAAGAGGT+GGG 0.461733 7.4:-25228080 None:intergenic
CATTTGCATTTGAAAGAAAG+AGG 0.462822 7.4:+25228550 MS.gene033571:CDS
GGACAATGGTGGACTAGCCG+GGG 0.462959 7.4:-25228058 None:intergenic
GGTTTCTTGAGGAAGTGGTT+GGG 0.466379 7.4:+25228344 MS.gene033571:CDS
GATGGTGAGGATGTGGTTGA+TGG 0.467160 7.4:-25227993 None:intergenic
TGGTGAAGAGGTGGGGACAA+TGG 0.476096 7.4:-25228072 None:intergenic
GTTGAGGTTTCTTGAGGAAG+TGG 0.478350 7.4:+25228339 MS.gene033571:CDS
CGGTGAAAGAGTGGGATTTG+AGG 0.484295 7.4:-25228024 None:intergenic
TATAGAGTTTGATGATTGAG+AGG 0.494375 7.4:-25227837 None:intergenic
AGAAGATGCGCATGAGGTTG+AGG 0.495323 7.4:+25228323 MS.gene033571:CDS
GGAGGTGAGGAAGGTGAAGA+CGG 0.499598 7.4:-25228122 None:intergenic
GAGTTTGATGATTGAGAGGG+AGG 0.502670 7.4:-25227833 None:intergenic
TTGGGTTTAGTTTGGAGAAG+AGG 0.509649 7.4:+25228515 MS.gene033571:CDS
GAGGAGGTCGGAGGTGAGGA+AGG 0.511277 7.4:-25228131 None:intergenic
AACCGTTATCCTAACAGAAA+CGG 0.513485 7.4:+25227866 MS.gene033571:CDS
AAGAAGATGGAGTTTGGCGT+TGG 0.515595 7.4:-25227808 None:intergenic
TGATGGTGTCAAGGTGAGGT+AGG 0.516204 7.4:-25227976 None:intergenic
TCACCGAGGCCGACATCCCC+CGG 0.518020 7.4:+25228041 MS.gene033571:CDS
GACAATGGTGGACTAGCCGG+GGG 0.519570 7.4:-25228057 None:intergenic
TTCACCAAATACACAAACTT+CGG 0.521850 7.4:-25228445 None:intergenic
CTTCCTCGCGGAGGAAGTGA+AGG 0.525212 7.4:-25228237 None:intergenic
AGATGCGCGTTCCTGTTAGT+TGG 0.527130 7.4:-25228283 None:intergenic
TGGCGAGGAGGTCGGAGGTG+AGG 0.543776 7.4:-25228135 None:intergenic
ATTTGGTGAAAGAGATGGAG+AGG 0.546039 7.4:+25228458 MS.gene033571:CDS
CACATCAACATTCTATTCAA+AGG 0.546735 7.4:-25228583 None:intergenic
TTTCTGTTAGGATAACGGTT+AGG 0.548102 7.4:-25227863 None:intergenic
CATCGGTGAAGAGGAGGTTA+GGG 0.549831 7.4:-25228192 None:intergenic
GGGAAATGGAGGTGGTGAAG+AGG 0.550003 7.4:-25228084 None:intergenic
GGTGAAAGAGATGGAGAGGG+AGG 0.550774 7.4:+25228462 MS.gene033571:CDS
ATAGAGTTTGATGATTGAGA+GGG 0.551408 7.4:-25227836 None:intergenic
TGAAGAGGAGGTTAGGGCAA+CGG 0.557020 7.4:-25228186 None:intergenic
CATGAGGTTGAGGTTTCTTG+AGG 0.557913 7.4:+25228333 MS.gene033571:CDS
CAAGGTTGCCGGCGATATTG+GGG 0.558881 7.4:+25228398 MS.gene033571:CDS
TCGCGGAGGAAGTGAAGGGT+TGG 0.561542 7.4:-25228232 None:intergenic
TGTCGGCCTCGGTGAAAGAG+TGG 0.565492 7.4:-25228033 None:intergenic
ATGTGATGATGGTGAGGATG+TGG 0.567993 7.4:-25228000 None:intergenic
TGTGTATTTGGTGAAAGAGA+TGG 0.569565 7.4:+25228453 MS.gene033571:CDS
TGGAGAAGAGGATTGTTCCA+AGG 0.570343 7.4:+25228527 MS.gene033571:CDS
GGTTTACGTACGAGGAAGCA+AGG 0.575985 7.4:+25228365 MS.gene033571:CDS
CTTTGAATAGAATGTTGATG+TGG 0.576656 7.4:+25228584 MS.gene033571:CDS
TTTGAATAGAATGTTGATGT+GGG 0.578021 7.4:+25228585 MS.gene033571:CDS
CACAGTGGCGAGGAGGTCGG+AGG 0.578035 7.4:-25228140 None:intergenic
TTTGGTGAAAGAGATGGAGA+GGG 0.581785 7.4:+25228459 MS.gene033571:CDS
ATGGAGGTGGTGAAGAGGTG+GGG 0.583863 7.4:-25228079 None:intergenic
TGAGGAATGTGATGATGGTG+AGG 0.588656 7.4:-25228006 None:intergenic
TTCCACAGTGGCGAGGAGGT+CGG 0.594168 7.4:-25228143 None:intergenic
AAGCAAGGAATGTTTGTGCA+AGG 0.594618 7.4:+25228380 MS.gene033571:CDS
GGATTTGAGGAATGTGATGA+TGG 0.599559 7.4:-25228011 None:intergenic
GGATGTGGTTGATGGTGTCA+AGG 0.602083 7.4:-25227985 None:intergenic
AGTGGTTGGGTTTACGTACG+AGG 0.606381 7.4:+25228357 MS.gene033571:CDS
ATGATAAGTTCTATGCAAAG+TGG 0.608848 7.4:+25228611 MS.gene033571:CDS
ACATCGTACCCCAATATCGC+CGG 0.610968 7.4:-25228406 None:intergenic
TGTGGTTTACATCGGTGAAG+AGG 0.614653 7.4:-25228201 None:intergenic
GTCGGCCTCGGTGAAAGAGT+GGG 0.623113 7.4:-25228032 None:intergenic
GGTTTACATCGGTGAAGAGG+AGG 0.624023 7.4:-25228198 None:intergenic
GGCGATATTGGGGTACGATG+TGG 0.628281 7.4:+25228408 MS.gene033571:CDS
CTCCGACCTCCTCGCCACTG+TGG 0.630362 7.4:+25228141 MS.gene033571:CDS
TGGTTGATGGTGTCAAGGTG+AGG 0.634445 7.4:-25227980 None:intergenic
TTTGAGGATGTGGTTTACAT+CGG 0.646901 7.4:-25228209 None:intergenic
AGGTGAGGAAGGTGAAGACG+GGG 0.649652 7.4:-25228120 None:intergenic
TTCCTCGCGGAGGAAGTGAA+GGG 0.649942 7.4:-25228236 None:intergenic
TGAAGAGGTGGGGACAATGG+TGG 0.654650 7.4:-25228069 None:intergenic
GAGAGTCTTCCACAGTGGCG+AGG 0.656874 7.4:-25228150 None:intergenic
GCATCTTCTCAACACGCGTG+TGG 0.668292 7.4:+25228300 MS.gene033571:CDS
AGTCTTCCACAGTGGCGAGG+AGG 0.672229 7.4:-25228147 None:intergenic
AGAGGAGGTTAGGGCAACGG+AGG 0.682019 7.4:-25228183 None:intergenic
GGTGAGGAAGGTGAAGACGG+GGG 0.684279 7.4:-25228119 None:intergenic
GTGTGGAGAAGATGCGCATG+AGG 0.685391 7.4:+25228317 MS.gene033571:CDS
AACCCTTCACTTCCTCCGCG+AGG 0.704917 7.4:+25228234 MS.gene033571:CDS
ACGATGTGGAGAATAACTTG+TGG 0.706381 7.4:+25228422 MS.gene033571:CDS
GCCACTGTGGAAGACTCTCG+CGG 0.706536 7.4:+25228154 MS.gene033571:CDS
TACTAACTCCAACTTCCTCG+CGG 0.708475 7.4:-25228249 None:intergenic
TTGAATAGAATGTTGATGTG+GGG 0.710612 7.4:+25228586 MS.gene033571:CDS
TAACTCCAACTTCCTCGCGG+AGG 0.712692 7.4:-25228246 None:intergenic
GCCGCGAGAGTCTTCCACAG+TGG 0.750300 7.4:-25228155 None:intergenic
CAAATCCCACTCTTTCACCG+AGG 0.751367 7.4:+25228027 MS.gene033571:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTATGGTTAGTGATTTT+AGG - chr7.4:25227934-25227953 None:intergenic 20.0%
! TTTGAATAGAATGTTGATGT+GGG + chr7.4:25228585-25228604 MS.gene033571:CDS 25.0%
AAACTAAACCCAAAAAACTG+CGG - chr7.4:25228508-25228527 None:intergenic 30.0%
ATGATAAGTTCTATGCAAAG+TGG + chr7.4:25228611-25228630 MS.gene033571:CDS 30.0%
CACATCAACATTCTATTCAA+AGG - chr7.4:25228586-25228605 None:intergenic 30.0%
CATTTGCATTTGAAAGAAAG+AGG + chr7.4:25228550-25228569 MS.gene033571:CDS 30.0%
CTTTGAATAGAATGTTGATG+TGG + chr7.4:25228584-25228603 MS.gene033571:CDS 30.0%
TTCACCAAATACACAAACTT+CGG - chr7.4:25228448-25228467 None:intergenic 30.0%
TTGAATAGAATGTTGATGTG+GGG + chr7.4:25228586-25228605 MS.gene033571:CDS 30.0%
! ATAGAGTTTGATGATTGAGA+GGG - chr7.4:25227839-25227858 None:intergenic 30.0%
! TATAGAGTTTGATGATTGAG+AGG - chr7.4:25227840-25227859 None:intergenic 30.0%
! TTTTGTGTTGGGGTTTATTA+TGG - chr7.4:25227948-25227967 None:intergenic 30.0%
!! ATTTGAAAGAAAGAGGTGTT+AGG + chr7.4:25228557-25228576 MS.gene033571:CDS 30.0%
!! GTAATGGAGATTTTCATTGT+AGG - chr7.4:25227912-25227931 None:intergenic 30.0%
!! TGGTTAGTGATTTTAGGTAA+TGG - chr7.4:25227928-25227947 None:intergenic 30.0%
AACCGTTATCCTAACAGAAA+CGG + chr7.4:25227866-25227885 MS.gene033571:CDS 35.0%
AACCGTTTCTGTTAGGATAA+CGG - chr7.4:25227871-25227890 None:intergenic 35.0%
TCTTTCAAATGCAAATGCCT+TGG - chr7.4:25228547-25228566 None:intergenic 35.0%
TTGATGTAACCGTTTCTGTT+AGG - chr7.4:25227878-25227897 None:intergenic 35.0%
TTTGAGGATGTGGTTTACAT+CGG - chr7.4:25228212-25228231 None:intergenic 35.0%
! TGTGTATTTGGTGAAAGAGA+TGG + chr7.4:25228453-25228472 MS.gene033571:CDS 35.0%
! TTTCTGTTAGGATAACGGTT+AGG - chr7.4:25227866-25227885 None:intergenic 35.0%
!!! AAGAAGTTTCCGCAGTTTTT+TGG + chr7.4:25228496-25228515 MS.gene033571:CDS 35.0%
!!! AGAAGTTTCCGCAGTTTTTT+GGG + chr7.4:25228497-25228516 MS.gene033571:CDS 35.0%
!!! AGGTTGTTTGGTTTTGTGTT+GGG - chr7.4:25227959-25227978 None:intergenic 35.0%
!!! GCAGTTTTTTGGGTTTAGTT+TGG + chr7.4:25228507-25228526 MS.gene033571:CDS 35.0%
!!! TAGGTTGTTTGGTTTTGTGT+TGG - chr7.4:25227960-25227979 None:intergenic 35.0%
AAGCAAGGAATGTTTGTGCA+AGG + chr7.4:25228380-25228399 MS.gene033571:CDS 40.0%
ACGATGTGGAGAATAACTTG+TGG + chr7.4:25228422-25228441 MS.gene033571:CDS 40.0%
GAGGATAAGAAGATGGAGTT+TGG - chr7.4:25227817-25227836 None:intergenic 40.0%
GGATTTGAGGAATGTGATGA+TGG - chr7.4:25228014-25228033 None:intergenic 40.0%
TTGGAGTTAGTAACCTGAAC+AGG + chr7.4:25228260-25228279 MS.gene033571:CDS 40.0%
TTTGGTGAAAGAGATGGAGA+GGG + chr7.4:25228459-25228478 MS.gene033571:CDS 40.0%
! ATTTGGTGAAAGAGATGGAG+AGG + chr7.4:25228458-25228477 MS.gene033571:CDS 40.0%
!! TTGGGTTTAGTTTGGAGAAG+AGG + chr7.4:25228515-25228534 MS.gene033571:CDS 40.0%
!!! GGTTGTTTGGTTTTGTGTTG+GGG - chr7.4:25227958-25227977 None:intergenic 40.0%
AAGAAGATGGAGTTTGGCGT+TGG - chr7.4:25227811-25227830 None:intergenic 45.0%
AGGTTTCTTGAGGAAGTGGT+TGG + chr7.4:25228343-25228362 MS.gene033571:CDS 45.0%
CATGAGGTTGAGGTTTCTTG+AGG + chr7.4:25228333-25228352 MS.gene033571:CDS 45.0%
GAATGTTTGTGCAAGGTTGC+CGG + chr7.4:25228387-25228406 MS.gene033571:CDS 45.0%
GGTTTCTTGAGGAAGTGGTT+GGG + chr7.4:25228344-25228363 MS.gene033571:CDS 45.0%
GTGGCCGAAGTTTGTGTATT+TGG + chr7.4:25228441-25228460 MS.gene033571:CDS 45.0%
GTTGAGGTTTCTTGAGGAAG+TGG + chr7.4:25228339-25228358 MS.gene033571:CDS 45.0%
TACTAACTCCAACTTCCTCG+CGG - chr7.4:25228252-25228271 None:intergenic 45.0%
TCAAGGTGAGGTAGGTTGTT+TGG - chr7.4:25227971-25227990 None:intergenic 45.0%
TGAGAGGGAGGATAAGAAGA+TGG - chr7.4:25227824-25227843 None:intergenic 45.0%
TGGAGAAGAGGATTGTTCCA+AGG + chr7.4:25228527-25228546 MS.gene033571:CDS 45.0%
TGTGGTTTACATCGGTGAAG+AGG - chr7.4:25228204-25228223 None:intergenic 45.0%
! GAGTTTGATGATTGAGAGGG+AGG - chr7.4:25227836-25227855 None:intergenic 45.0%
!! ATGTGATGATGGTGAGGATG+TGG - chr7.4:25228003-25228022 None:intergenic 45.0%
!! TGAGGAATGTGATGATGGTG+AGG - chr7.4:25228009-25228028 None:intergenic 45.0%
!! TTTGGCGTTGGTGGAAAGAT+TGG - chr7.4:25227799-25227818 None:intergenic 45.0%
AAATGGAGGTGGTGAAGAGG+TGG - chr7.4:25228084-25228103 None:intergenic 50.0%
AATGGAGGTGGTGAAGAGGT+GGG - chr7.4:25228083-25228102 None:intergenic 50.0%
ACATCGGTGAAGAGGAGGTT+AGG - chr7.4:25228196-25228215 None:intergenic 50.0%
ACATCGTACCCCAATATCGC+CGG - chr7.4:25228409-25228428 None:intergenic 50.0%
ACCTGAACAGGCCAACTAAC+AGG + chr7.4:25228272-25228291 MS.gene033571:CDS 50.0%
AGAAGATGCGCATGAGGTTG+AGG + chr7.4:25228323-25228342 MS.gene033571:CDS 50.0%
AGATGCGCGTTCCTGTTAGT+TGG - chr7.4:25228286-25228305 None:intergenic 50.0%
AGTGGTTGGGTTTACGTACG+AGG + chr7.4:25228357-25228376 MS.gene033571:CDS 50.0%
CAAATCCCACTCTTTCACCG+AGG + chr7.4:25228027-25228046 MS.gene033571:CDS 50.0%
CATCGGTGAAGAGGAGGTTA+GGG - chr7.4:25228195-25228214 None:intergenic 50.0%
CGGTGAAAGAGTGGGATTTG+AGG - chr7.4:25228027-25228046 None:intergenic 50.0%
GATGGTGAGGATGTGGTTGA+TGG - chr7.4:25227996-25228015 None:intergenic 50.0%
GGTTTACATCGGTGAAGAGG+AGG - chr7.4:25228201-25228220 None:intergenic 50.0%
GGTTTACGTACGAGGAAGCA+AGG + chr7.4:25228365-25228384 MS.gene033571:CDS 50.0%
TGAAGAGGAGGTTAGGGCAA+CGG - chr7.4:25228189-25228208 None:intergenic 50.0%
! AAGATGGAGTTTGGCGTTGG+TGG - chr7.4:25227808-25227827 None:intergenic 50.0%
! TCCTGTTAGTTGGCCTGTTC+AGG - chr7.4:25228276-25228295 None:intergenic 50.0%
! TGATGGTGTCAAGGTGAGGT+AGG - chr7.4:25227979-25227998 None:intergenic 50.0%
!! GAAGGGTTGGTTTGAGGATG+TGG - chr7.4:25228222-25228241 None:intergenic 50.0%
!! GGAAGTGAAGGGTTGGTTTG+AGG - chr7.4:25228228-25228247 None:intergenic 50.0%
!! GGATGTGGTTGATGGTGTCA+AGG - chr7.4:25227988-25228007 None:intergenic 50.0%
!! TGGTTGATGGTGTCAAGGTG+AGG - chr7.4:25227983-25228002 None:intergenic 50.0%
AGGTGAGGAAGGTGAAGACG+GGG - chr7.4:25228123-25228142 None:intergenic 55.0%
ATGGAGGTGGTGAAGAGGTG+GGG - chr7.4:25228082-25228101 None:intergenic 55.0%
CAAGGTTGCCGGCGATATTG+GGG + chr7.4:25228398-25228417 MS.gene033571:CDS 55.0%
GAAAGAGATGGAGAGGGAGG+TGG + chr7.4:25228465-25228484 MS.gene033571:CDS 55.0%
GAGGTGAGGAAGGTGAAGAC+GGG - chr7.4:25228124-25228143 None:intergenic 55.0%
GCAAGGTTGCCGGCGATATT+GGG + chr7.4:25228397-25228416 MS.gene033571:CDS 55.0%
GCATCTTCTCAACACGCGTG+TGG + chr7.4:25228300-25228319 MS.gene033571:CDS 55.0%
GGAGGTGAGGAAGGTGAAGA+CGG - chr7.4:25228125-25228144 None:intergenic 55.0%
GGCGATATTGGGGTACGATG+TGG + chr7.4:25228408-25228427 MS.gene033571:CDS 55.0%
GGGAAATGGAGGTGGTGAAG+AGG - chr7.4:25228087-25228106 None:intergenic 55.0%
GGTGAAAGAGATGGAGAGGG+AGG + chr7.4:25228462-25228481 MS.gene033571:CDS 55.0%
GGTTAGGGCAACGGAGGATA+AGG - chr7.4:25228180-25228199 None:intergenic 55.0%
GTGTGGAGAAGATGCGCATG+AGG + chr7.4:25228317-25228336 MS.gene033571:CDS 55.0%
TAACTCCAACTTCCTCGCGG+AGG - chr7.4:25228249-25228268 None:intergenic 55.0%
TGAAGAGGTGGGGACAATGG+TGG - chr7.4:25228072-25228091 None:intergenic 55.0%
TGCAAGGTTGCCGGCGATAT+TGG + chr7.4:25228396-25228415 MS.gene033571:CDS 55.0%
TGGTGAAGAGGTGGGGACAA+TGG - chr7.4:25228075-25228094 None:intergenic 55.0%
TTCCTCGCGGAGGAAGTGAA+GGG - chr7.4:25228239-25228258 None:intergenic 55.0%
AACCCTTCACTTCCTCCGCG+AGG + chr7.4:25228234-25228253 MS.gene033571:CDS 60.0%
AGAGGAGGTTAGGGCAACGG+AGG - chr7.4:25228186-25228205 None:intergenic 60.0%
AGTCTTCCACAGTGGCGAGG+AGG - chr7.4:25228150-25228169 None:intergenic 60.0%
CACTTCCTCCGCGAGGAAGT+TGG + chr7.4:25228241-25228260 MS.gene033571:CDS 60.0%
CTTCCTCGCGGAGGAAGTGA+AGG - chr7.4:25228240-25228259 None:intergenic 60.0%
GAAGGTGAAGACGGGGGAGA+GGG - chr7.4:25228116-25228135 None:intergenic 60.0%
GAGAGTCTTCCACAGTGGCG+AGG - chr7.4:25228153-25228172 None:intergenic 60.0%
GAGGGAGGTAGGGGAAATGG+AGG - chr7.4:25228098-25228117 None:intergenic 60.0%
GCCACTGTGGAAGACTCTCG+CGG + chr7.4:25228154-25228173 MS.gene033571:CDS 60.0%
GGAGAGGGAGGTAGGGGAAA+TGG - chr7.4:25228101-25228120 None:intergenic 60.0%
GGAGGTAGGGGAAATGGAGG+TGG - chr7.4:25228095-25228114 None:intergenic 60.0%
GGTGAGGAAGGTGAAGACGG+GGG - chr7.4:25228122-25228141 None:intergenic 60.0%
GTCGGCCTCGGTGAAAGAGT+GGG - chr7.4:25228035-25228054 None:intergenic 60.0%
TCGCGGAGGAAGTGAAGGGT+TGG - chr7.4:25228235-25228254 None:intergenic 60.0%
TGTCGGCCTCGGTGAAAGAG+TGG - chr7.4:25228036-25228055 None:intergenic 60.0%
TTCCACAGTGGCGAGGAGGT+CGG - chr7.4:25228146-25228165 None:intergenic 60.0%
!! GACAATGGTGGACTAGCCGG+GGG - chr7.4:25228060-25228079 None:intergenic 60.0%
!! GGACAATGGTGGACTAGCCG+GGG - chr7.4:25228061-25228080 None:intergenic 60.0%
!! GGGACAATGGTGGACTAGCC+GGG - chr7.4:25228062-25228081 None:intergenic 60.0%
!! GGGGACAATGGTGGACTAGC+CGG - chr7.4:25228063-25228082 None:intergenic 60.0%
AAGACGGGGGAGAGGGAGGT+AGG - chr7.4:25228109-25228128 None:intergenic 65.0%
AGACGGGGGAGAGGGAGGTA+GGG - chr7.4:25228108-25228127 None:intergenic 65.0%
GAGGAGGTCGGAGGTGAGGA+AGG - chr7.4:25228134-25228153 None:intergenic 65.0%
GCCGCGAGAGTCTTCCACAG+TGG - chr7.4:25228158-25228177 None:intergenic 65.0%
GGAAGGTGAAGACGGGGGAG+AGG - chr7.4:25228117-25228136 None:intergenic 65.0%
!! GTGGACTAGCCGGGGGATGT+CGG - chr7.4:25228053-25228072 None:intergenic 65.0%
CACAGTGGCGAGGAGGTCGG+AGG - chr7.4:25228143-25228162 None:intergenic 70.0%
CTCCGACCTCCTCGCCACTG+TGG + chr7.4:25228141-25228160 MS.gene033571:CDS 70.0%
GACGGGGGAGAGGGAGGTAG+GGG - chr7.4:25228107-25228126 None:intergenic 70.0%
GGTGAAGACGGGGGAGAGGG+AGG - chr7.4:25228113-25228132 None:intergenic 70.0%
TAGCCGGGGGATGTCGGCCT+CGG - chr7.4:25228047-25228066 None:intergenic 70.0%
TCACCGAGGCCGACATCCCC+CGG + chr7.4:25228041-25228060 MS.gene033571:CDS 70.0%
TGGCGAGGAGGTCGGAGGTG+AGG - chr7.4:25228138-25228157 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 25227785 25228639 25227785 ID=MS.gene033571
chr7.4 mRNA 25227785 25228639 25227785 ID=MS.gene033571.t1;Parent=MS.gene033571
chr7.4 exon 25227785 25228639 25227785 ID=MS.gene033571.t1.exon1;Parent=MS.gene033571.t1
chr7.4 CDS 25227785 25228639 25227785 ID=cds.MS.gene033571.t1;Parent=MS.gene033571.t1
Gene Sequence

>MS.gene033571

ATGTTCTTCGTCCAATCTTTCCACCAACGCCAAACTCCATCTTCTTATCCTCCCTCTCAATCATCAAACTCTATAGTACCTAACCGTTATCCTAACAGAAACGGTTACATCAAGTTCAGAACAACCTACAATGAAAATCTCCATTACCTAAAATCACTAACCATAATAAACCCCAACACAAAACCAAACAACCTACCTCACCTTGACACCATCAACCACATCCTCACCATCATCACATTCCTCAAATCCCACTCTTTCACCGAGGCCGACATCCCCCGGCTAGTCCACCATTGTCCCCACCTCTTCACCACCTCCATTTCCCCTACCTCCCTCTCCCCCGTCTTCACCTTCCTCACCTCCGACCTCCTCGCCACTGTGGAAGACTCTCGCGGCCTTATCCTCCGTTGCCCTAACCTCCTCTTCACCGATGTAAACCACATCCTCAAACCAACCCTTCACTTCCTCCGCGAGGAAGTTGGAGTTAGTAACCTGAACAGGCCAACTAACAGGAACGCGCATCTTCTCAACACGCGTGTGGAGAAGATGCGCATGAGGTTGAGGTTTCTTGAGGAAGTGGTTGGGTTTACGTACGAGGAAGCAAGGAATGTTTGTGCAAGGTTGCCGGCGATATTGGGGTACGATGTGGAGAATAACTTGTGGCCGAAGTTTGTGTATTTGGTGAAAGAGATGGAGAGGGAGGTGGAAGAGTTGAAGAAGTTTCCGCAGTTTTTTGGGTTTAGTTTGGAGAAGAGGATTGTTCCAAGGCATTTGCATTTGAAAGAAAGAGGTGTTAGGATTCCTTTGAATAGAATGTTGATGTGGGGTGATGATAAGTTCTATGCAAAGTGGAAGTGA
Protein sequence

>MS.gene033571.t1

MFFVQSFHQRQTPSSYPPSQSSNSIVPNRYPNRNGYIKFRTTYNENLHYLKSLTIINPNTKPNNLPHLDTINHILTIITFLKSHSFTEADIPRLVHHCPHLFTTSISPTSLSPVFTFLTSDLLATVEDSRGLILRCPNLLFTDVNHILKPTLHFLREEVGVSNLNRPTNRNAHLLNTRVEKMRMRLRFLEEVVGFTYEEARNVCARLPAILGYDVENNLWPKFVYLVKEMEREVEELKKFPQFFGFSLEKRIVPRHLHLKERGVRIPLNRMLMWGDDKFYAKWK