Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033664.t1 | XP_003624437.1 | 83 | 200 | 21 | 5 | 1 | 199 | 1 | 188 | 1.10E-39 | 173.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033664.t1 | Q9FFX4 | 87.9 | 33 | 4 | 0 | 40 | 72 | 36 | 68 | 3.9e-09 | 63.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033664.t1 | G7KW55 | 83.0 | 200 | 21 | 5 | 1 | 199 | 1 | 188 | 8.0e-40 | 173.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene033664.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033664.t1 | MTR_7g083210 | 77.000 | 200 | 33 | 7 | 1 | 199 | 1 | 188 | 1.88e-89 | 260 |
| MS.gene033664.t1 | MTR_7g086860 | 32.381 | 210 | 74 | 7 | 1 | 199 | 1 | 153 | 1.30e-19 | 81.6 |
| MS.gene033664.t1 | MTR_1g107055 | 52.830 | 53 | 19 | 1 | 39 | 85 | 63 | 115 | 1.98e-12 | 63.5 |
| MS.gene033664.t1 | MTR_1g013130 | 75.758 | 33 | 8 | 0 | 40 | 72 | 94 | 126 | 7.35e-12 | 62.8 |
| MS.gene033664.t1 | MTR_4g128340 | 75.758 | 33 | 8 | 0 | 40 | 72 | 45 | 77 | 9.23e-12 | 61.2 |
| MS.gene033664.t1 | MTR_3g065020 | 72.727 | 33 | 9 | 0 | 40 | 72 | 86 | 118 | 5.54e-11 | 60.1 |
| MS.gene033664.t1 | MTR_4g006575 | 75.758 | 33 | 8 | 0 | 40 | 72 | 67 | 99 | 5.71e-11 | 60.1 |
| MS.gene033664.t1 | MTR_4g006600 | 75.758 | 33 | 8 | 0 | 40 | 72 | 67 | 99 | 5.71e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033664.t1 | AT5G14010 | 66.000 | 50 | 17 | 0 | 40 | 89 | 36 | 85 | 7.11e-15 | 69.3 |
| MS.gene033664.t1 | AT5G57520 | 54.545 | 55 | 24 | 1 | 40 | 94 | 50 | 103 | 1.10e-13 | 65.9 |
| MS.gene033664.t1 | AT1G24625 | 72.727 | 33 | 9 | 0 | 40 | 72 | 57 | 89 | 9.42e-11 | 59.3 |
Find 38 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATCATCATTACCCTTAT+TGG | 0.093745 | 7.4:+23732097 | MS.gene033664:CDS |
| TATGGTTATGCTTCTGGTTT+TGG | 0.247711 | 7.4:+23732210 | MS.gene033664:CDS |
| GGTTCCATTTGTTGCCAATA+AGG | 0.254384 | 7.4:-23732111 | None:intergenic |
| TTTCAAGTTTGTGAGCATTT+TGG | 0.265375 | 7.4:-23731942 | None:intergenic |
| GATCTTCTGCTAGTTGTTCT+TGG | 0.275259 | 7.4:-23731826 | None:intergenic |
| ATGCTTCCTTGCAATGGTTA+TGG | 0.317002 | 7.4:+23732192 | MS.gene033664:CDS |
| GGTGGTGTTGATCAGGTTGA+TGG | 0.328750 | 7.4:-23731986 | None:intergenic |
| AATGGTTATGGTTATGCTTC+TGG | 0.361952 | 7.4:+23732204 | MS.gene033664:CDS |
| GTTCCATTTGTTGCCAATAA+GGG | 0.373916 | 7.4:-23732110 | None:intergenic |
| AGAAGCAGAAGCAGCATTGT+TGG | 0.377902 | 7.4:-23732271 | None:intergenic |
| TGTTGGTGGTGGTGTTGATC+AGG | 0.386212 | 7.4:-23731993 | None:intergenic |
| TGTGGTGTTTGAAACTGTAT+AGG | 0.418717 | 7.4:-23732132 | None:intergenic |
| TTACCCTTATTGGCAACAAA+TGG | 0.422059 | 7.4:+23732107 | MS.gene033664:CDS |
| AACATTGTTGGTGTTGTTGT+TGG | 0.428219 | 7.4:-23732010 | None:intergenic |
| GTTGGTGTTGTTGTTGGTGG+TGG | 0.434485 | 7.4:-23732004 | None:intergenic |
| TGTGGTGGTGGTGATGAAAA+AGG | 0.435180 | 7.4:-23732036 | None:intergenic |
| TTTCATGAGGAACTCGTAGA+TGG | 0.442852 | 7.4:-23731779 | None:intergenic |
| CATCCTATGCTTCCTTGCAA+TGG | 0.462936 | 7.4:+23732186 | MS.gene033664:CDS |
| TTCTACACTTCTCAAGCTCT+TGG | 0.466221 | 7.4:+23731913 | MS.gene033664:CDS |
| TACACTTCTCAAGCTCTTGG+TGG | 0.467223 | 7.4:+23731916 | MS.gene033664:CDS |
| ATTGTTGGTGTTGTTGTTGG+TGG | 0.489563 | 7.4:-23732007 | None:intergenic |
| CTTCTGCTAGTTGTTCTTGG+TGG | 0.497901 | 7.4:-23731823 | None:intergenic |
| CTTGAGAAGTGTAGAACTTA+CGG | 0.498780 | 7.4:-23731906 | None:intergenic |
| GCTTCAACATGTTGATGTTG+TGG | 0.525749 | 7.4:-23732150 | None:intergenic |
| AGGAACTCGTAGATGGCTGA+AGG | 0.562270 | 7.4:-23731772 | None:intergenic |
| TAACCATTGCAAGGAAGCAT+AGG | 0.562677 | 7.4:-23732189 | None:intergenic |
| GAAACATGTGACTGTGGTGG+TGG | 0.592935 | 7.4:-23732048 | None:intergenic |
| AAGTTTGTTGTTGTTTCATG+AGG | 0.595946 | 7.4:-23731792 | None:intergenic |
| GCATAACCATAACCATTGCA+AGG | 0.611111 | 7.4:-23732198 | None:intergenic |
| TTCTTCAGAGATGAAGAGTG+AGG | 0.651759 | 7.4:-23732344 | None:intergenic |
| GTTTCTGAACAACATGAACA+AGG | 0.674380 | 7.4:+23732066 | MS.gene033664:CDS |
| GGAACTCGTAGATGGCTGAA+GGG | 0.682433 | 7.4:-23731771 | None:intergenic |
| TTGTTGGTGAAAACATAGTG+AGG | 0.685351 | 7.4:-23732255 | None:intergenic |
| TCTGAACAACATGAACAAGG+TGG | 0.702272 | 7.4:+23732069 | MS.gene033664:CDS |
| TCAGAAGCATCAACAAACTG+AGG | 0.702630 | 7.4:-23732306 | None:intergenic |
| GAGTGAGGTCAAGATTGACA+TGG | 0.709894 | 7.4:-23732329 | None:intergenic |
| TCAGAAACATGTGACTGTGG+TGG | 0.727680 | 7.4:-23732051 | None:intergenic |
| TGTTCAGAAACATGTGACTG+TGG | 0.756060 | 7.4:-23732054 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGAAAAAGGAACAACATTGT+TGG | - | chr7.4:23732025-23732044 | None:intergenic | 30.0% | |
| TTCATCATCATTACCCTTAT+TGG | + | chr7.4:23732097-23732116 | MS.gene033664:CDS | 30.0% | |
| !! | AAGTTTGTTGTTGTTTCATG+AGG | - | chr7.4:23731795-23731814 | None:intergenic | 30.0% |
| !!! | TTTCAAGTTTGTGAGCATTT+TGG | - | chr7.4:23731945-23731964 | None:intergenic | 30.0% |
| AATGGTTATGGTTATGCTTC+TGG | + | chr7.4:23732204-23732223 | MS.gene033664:CDS | 35.0% | |
| GTTTCTGAACAACATGAACA+AGG | + | chr7.4:23732066-23732085 | MS.gene033664:CDS | 35.0% | |
| TTACCCTTATTGGCAACAAA+TGG | + | chr7.4:23732107-23732126 | MS.gene033664:CDS | 35.0% | |
| ! | CTTGAGAAGTGTAGAACTTA+CGG | - | chr7.4:23731909-23731928 | None:intergenic | 35.0% |
| ! | GTTCCATTTGTTGCCAATAA+GGG | - | chr7.4:23732113-23732132 | None:intergenic | 35.0% |
| ! | TGTGGTGTTTGAAACTGTAT+AGG | - | chr7.4:23732135-23732154 | None:intergenic | 35.0% |
| ! | TTGTTGGTGAAAACATAGTG+AGG | - | chr7.4:23732258-23732277 | None:intergenic | 35.0% |
| !! | AACATTGTTGGTGTTGTTGT+TGG | - | chr7.4:23732013-23732032 | None:intergenic | 35.0% |
| !!! | TATGCTTCTGGTTTTGGTTT+TGG | + | chr7.4:23732216-23732235 | MS.gene033664:CDS | 35.0% |
| !!! | TATGGTTATGCTTCTGGTTT+TGG | + | chr7.4:23732210-23732229 | MS.gene033664:CDS | 35.0% |
| ATGCTTCCTTGCAATGGTTA+TGG | + | chr7.4:23732192-23732211 | MS.gene033664:CDS | 40.0% | |
| GATCTTCTGCTAGTTGTTCT+TGG | - | chr7.4:23731829-23731848 | None:intergenic | 40.0% | |
| GCATAACCATAACCATTGCA+AGG | - | chr7.4:23732201-23732220 | None:intergenic | 40.0% | |
| GCTTCAACATGTTGATGTTG+TGG | - | chr7.4:23732153-23732172 | None:intergenic | 40.0% | |
| TCTGAACAACATGAACAAGG+TGG | + | chr7.4:23732069-23732088 | MS.gene033664:CDS | 40.0% | |
| TGTTCAGAAACATGTGACTG+TGG | - | chr7.4:23732057-23732076 | None:intergenic | 40.0% | |
| TTCTACACTTCTCAAGCTCT+TGG | + | chr7.4:23731913-23731932 | MS.gene033664:CDS | 40.0% | |
| TTTCATGAGGAACTCGTAGA+TGG | - | chr7.4:23731782-23731801 | None:intergenic | 40.0% | |
| ! | GGTTCCATTTGTTGCCAATA+AGG | - | chr7.4:23732114-23732133 | None:intergenic | 40.0% |
| ! | TAACCATTGCAAGGAAGCAT+AGG | - | chr7.4:23732192-23732211 | None:intergenic | 40.0% |
| !! | ATTGTTGGTGTTGTTGTTGG+TGG | - | chr7.4:23732010-23732029 | None:intergenic | 40.0% |
| !! | TCAGAAGCATCAACAAACTG+AGG | - | chr7.4:23732309-23732328 | None:intergenic | 40.0% |
| AGAAGCAGAAGCAGCATTGT+TGG | - | chr7.4:23732274-23732293 | None:intergenic | 45.0% | |
| CATCCTATGCTTCCTTGCAA+TGG | + | chr7.4:23732186-23732205 | MS.gene033664:CDS | 45.0% | |
| CTTCTGCTAGTTGTTCTTGG+TGG | - | chr7.4:23731826-23731845 | None:intergenic | 45.0% | |
| GAGTGAGGTCAAGATTGACA+TGG | - | chr7.4:23732332-23732351 | None:intergenic | 45.0% | |
| TACACTTCTCAAGCTCTTGG+TGG | + | chr7.4:23731916-23731935 | MS.gene033664:CDS | 45.0% | |
| TCAGAAACATGTGACTGTGG+TGG | - | chr7.4:23732054-23732073 | None:intergenic | 45.0% | |
| !! | TGTGGTGGTGGTGATGAAAA+AGG | - | chr7.4:23732039-23732058 | None:intergenic | 45.0% |
| AGGAACTCGTAGATGGCTGA+AGG | - | chr7.4:23731775-23731794 | None:intergenic | 50.0% | |
| GAAACATGTGACTGTGGTGG+TGG | - | chr7.4:23732051-23732070 | None:intergenic | 50.0% | |
| GGAACTCGTAGATGGCTGAA+GGG | - | chr7.4:23731774-23731793 | None:intergenic | 50.0% | |
| !! | GGTGGTGTTGATCAGGTTGA+TGG | - | chr7.4:23731989-23732008 | None:intergenic | 50.0% |
| !! | GTTGGTGTTGTTGTTGGTGG+TGG | - | chr7.4:23732007-23732026 | None:intergenic | 50.0% |
| !! | TGTTGGTGGTGGTGTTGATC+AGG | - | chr7.4:23731996-23732015 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 23731763 | 23732362 | 23731763 | ID=MS.gene033664 |
| chr7.4 | mRNA | 23731763 | 23732362 | 23731763 | ID=MS.gene033664.t1;Parent=MS.gene033664 |
| chr7.4 | exon | 23731763 | 23732362 | 23731763 | ID=MS.gene033664.t1.exon1;Parent=MS.gene033664.t1 |
| chr7.4 | CDS | 23731763 | 23732362 | 23731763 | ID=cds.MS.gene033664.t1;Parent=MS.gene033664.t1 |
| Gene Sequence |
| Protein sequence |
