AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033668

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033668.t1 MTR_7g083330 93.367 196 11 2 1 196 1 194 1.94e-136 379
MS.gene033668.t1 MTR_7g083360 70.000 150 43 2 16 165 8 155 5.18e-74 220
MS.gene033668.t1 MTR_7g083370 74.803 127 31 1 18 144 10 135 8.60e-68 204
MS.gene033668.t1 MTR_2g064090 32.911 158 98 5 16 167 4 159 6.66e-21 88.6
MS.gene033668.t1 MTR_6g477900 32.298 161 102 5 16 171 4 162 1.65e-20 85.9
MS.gene033668.t1 MTR_4g089135 34.932 146 84 6 18 157 15 155 3.81e-19 84.0
MS.gene033668.t1 MTR_5g090970 37.778 135 75 6 13 142 14 144 5.39e-19 83.2
MS.gene033668.t1 MTR_2g068880 31.383 188 93 9 13 174 18 195 1.63e-18 81.6
MS.gene033668.t1 MTR_5g076850 35.606 132 76 5 16 142 4 131 3.82e-18 82.0
MS.gene033668.t1 MTR_2g068920 36.364 143 74 7 10 142 14 149 3.88e-18 80.5
MS.gene033668.t1 MTR_3g064580 34.848 132 77 5 16 142 4 131 7.12e-18 81.3
MS.gene033668.t1 MTR_5g090970 36.296 135 76 6 13 142 14 143 1.42e-17 79.3
MS.gene033668.t1 MTR_6g011860 31.507 146 94 4 5 147 2 144 1.54e-17 79.7
MS.gene033668.t1 MTR_2157s0010 30.216 139 89 5 18 151 8 143 2.41e-17 77.8
MS.gene033668.t1 MTR_5g041940 29.193 161 105 6 16 173 1 155 3.71e-17 77.4
MS.gene033668.t1 MTR_8g094580 30.216 139 89 5 12 147 1 134 4.15e-17 77.8
MS.gene033668.t1 MTR_3g070030 35.135 148 84 8 1 142 1 142 4.98e-17 78.2
MS.gene033668.t1 MTR_5g040420 33.094 139 85 6 14 148 11 145 6.72e-17 78.2
MS.gene033668.t1 MTR_3g096920 31.655 139 87 5 12 147 1 134 1.25e-16 76.3
MS.gene033668.t1 MTR_7g085220 31.618 136 79 5 18 142 8 140 1.26e-16 77.0
MS.gene033668.t1 MTR_3g088110 31.852 135 84 4 16 147 5 134 1.43e-16 76.3
MS.gene033668.t1 MTR_1g008740 33.333 132 80 6 18 144 13 141 1.54e-16 76.6
MS.gene033668.t1 MTR_1g008740 33.333 132 80 6 18 144 27 155 1.66e-16 76.6
MS.gene033668.t1 MTR_3g109340 32.877 146 88 7 6 144 7 149 1.77e-16 76.6
MS.gene033668.t1 MTR_3g109340 32.877 146 88 7 6 144 7 149 1.90e-16 76.6
MS.gene033668.t1 MTR_3g096920 31.655 139 87 5 12 147 1 134 1.91e-16 76.3
MS.gene033668.t1 MTR_2g080010 34.351 131 75 6 18 142 15 140 2.05e-16 76.3
MS.gene033668.t1 MTR_4g081870 28.571 161 104 7 17 173 8 161 3.12e-16 75.1
MS.gene033668.t1 MTR_1g097300 30.601 183 109 7 2 177 3 174 3.23e-16 76.3
MS.gene033668.t1 MTR_6g032770 32.824 131 81 5 16 142 16 143 3.27e-16 75.9
MS.gene033668.t1 MTR_1g069805 32.353 136 85 5 15 146 7 139 5.06e-16 74.3
MS.gene033668.t1 MTR_5g069030 29.231 195 123 6 5 186 12 204 6.11e-16 75.5
MS.gene033668.t1 MTR_7g005280 35.338 133 76 6 16 144 13 139 1.04e-15 73.6
MS.gene033668.t1 MTR_4g036030 28.750 160 108 5 18 173 7 164 1.55e-15 73.9
MS.gene033668.t1 MTR_2g078700 32.593 135 84 6 18 148 23 154 1.85e-15 73.9
MS.gene033668.t1 MTR_6g012670 34.932 146 87 7 1 142 2 143 1.90e-15 73.6
MS.gene033668.t1 MTR_8g023840 32.143 140 87 5 18 152 11 147 2.26e-15 73.6
MS.gene033668.t1 MTR_3g093040 32.117 137 88 4 9 142 99 233 2.51e-15 73.2
MS.gene033668.t1 MTR_8g467490 30.263 152 97 7 4 151 2 148 3.01e-15 73.2
MS.gene033668.t1 MTR_3g093050 33.571 140 82 5 12 142 10 147 3.49e-15 73.6
MS.gene033668.t1 MTR_3g093050 33.571 140 82 5 12 142 10 147 3.63e-15 73.6
MS.gene033668.t1 MTR_8g023900 32.308 130 81 4 18 142 12 139 7.08e-15 72.4
MS.gene033668.t1 MTR_6g084430 28.571 154 103 6 18 168 5 154 1.25e-14 71.2
MS.gene033668.t1 MTR_8g023880 28.000 150 85 4 12 142 1 146 1.60e-14 70.5
MS.gene033668.t1 MTR_3g116070 33.846 130 78 7 18 142 23 149 1.74e-14 70.9
MS.gene033668.t1 MTR_7g097090 32.117 137 84 6 15 146 7 139 1.98e-14 70.5
MS.gene033668.t1 MTR_4g108760 30.827 133 85 6 13 142 12 140 2.37e-14 70.5
MS.gene033668.t1 MTR_4g098630 27.485 171 118 3 14 179 2 171 2.77e-14 70.1
MS.gene033668.t1 MTR_3g435150 31.579 133 84 6 13 142 11 139 3.89e-14 69.7
MS.gene033668.t1 MTR_1g090723 30.464 151 94 7 21 164 11 157 4.04e-14 70.5
MS.gene033668.t1 MTR_7g085260 33.094 139 84 6 16 148 15 150 4.76e-14 69.7
MS.gene033668.t1 MTR_8g059170 32.824 131 79 6 16 142 12 137 4.99e-14 69.3
MS.gene033668.t1 MTR_8g023860 31.343 134 77 5 18 142 12 139 5.32e-14 69.7
MS.gene033668.t1 MTR_1g090723 30.464 151 94 7 21 164 11 157 5.74e-14 70.1
MS.gene033668.t1 MTR_8g076110 30.597 134 87 5 18 147 6 137 6.49e-14 68.9
MS.gene033668.t1 MTR_7g100990 29.050 179 104 9 7 177 6 169 6.73e-14 69.3
MS.gene033668.t1 MTR_2g093810 28.859 149 97 6 18 162 14 157 1.04e-13 68.6
MS.gene033668.t1 MTR_5g014300 29.240 171 101 7 15 174 3 164 1.13e-13 68.6
MS.gene033668.t1 MTR_3g096140 31.200 125 81 4 21 142 10 132 1.21e-13 68.2
MS.gene033668.t1 MTR_4g101680 28.571 161 105 7 18 172 7 163 1.62e-13 68.2
MS.gene033668.t1 MTR_5g012080 27.820 133 91 4 18 146 7 138 2.40e-13 67.8
MS.gene033668.t1 MTR_8g023930 32.090 134 76 5 18 142 24 151 2.51e-13 67.8
MS.gene033668.t1 MTR_1g090720 33.077 130 78 5 18 142 42 167 2.53e-13 67.8
MS.gene033668.t1 MTR_3g096140 31.200 125 81 4 21 142 10 132 2.89e-13 67.4
MS.gene033668.t1 MTR_2g079990 31.538 130 82 5 16 142 14 139 4.17e-13 67.0
MS.gene033668.t1 MTR_2g079990 31.538 130 82 5 16 142 14 139 4.19e-13 67.0
MS.gene033668.t1 MTR_1g090723 31.250 128 78 6 21 142 11 134 4.54e-13 67.4
MS.gene033668.t1 MTR_2g086880 34.109 129 75 7 21 144 21 144 6.65e-13 66.6
MS.gene033668.t1 MTR_2g086880 33.071 127 79 6 21 144 21 144 9.28e-13 66.2
MS.gene033668.t1 MTR_5g021710 30.233 129 84 5 18 142 8 134 1.36e-12 65.5
MS.gene033668.t1 MTR_0036s0150 28.188 149 100 5 21 164 8 154 1.79e-12 65.5
MS.gene033668.t1 MTR_2g062730 30.075 133 88 4 16 144 8 139 1.94e-12 65.1
MS.gene033668.t1 MTR_1g096430 28.477 151 99 6 18 161 7 155 3.44e-12 64.3
MS.gene033668.t1 MTR_8g099750 28.395 162 101 8 17 169 13 168 4.59e-12 63.2
MS.gene033668.t1 MTR_4g134460 30.201 149 94 6 21 164 57 200 9.91e-12 63.2
MS.gene033668.t1 MTR_4g035590 32.824 131 82 6 18 144 8 136 2.52e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033668.t1 AT3G10500 34.868 152 92 6 18 164 9 158 1.03e-21 92.4
MS.gene033668.t1 AT5G13180 36.957 138 77 5 9 142 5 136 2.69e-21 88.2
MS.gene033668.t1 AT5G04410 35.156 128 78 4 18 142 9 134 1.24e-20 89.4
MS.gene033668.t1 AT3G03200 33.540 161 99 5 16 170 4 162 2.81e-20 88.2
MS.gene033668.t1 AT5G17260 32.298 161 101 5 16 170 4 162 5.95e-20 87.0
MS.gene033668.t1 AT3G17730 35.659 129 80 3 16 142 4 131 8.13e-20 84.3
MS.gene033668.t1 AT1G65910 32.099 162 102 6 16 172 4 162 2.93e-19 85.5
MS.gene033668.t1 AT1G54330 33.537 164 99 5 16 174 4 162 3.12e-19 83.6
MS.gene033668.t1 AT5G39820 33.108 148 91 6 4 147 7 150 6.64e-19 83.2
MS.gene033668.t1 AT2G24430 34.884 129 77 6 18 142 16 141 9.22e-19 82.4
MS.gene033668.t1 AT2G24430 34.884 129 77 6 18 142 16 141 9.22e-19 82.4
MS.gene033668.t1 AT3G12910 36.879 141 77 6 13 146 17 152 1.07e-18 82.4
MS.gene033668.t1 AT1G76420 33.108 148 90 7 1 142 3 147 1.55e-18 82.0
MS.gene033668.t1 AT1G61110 34.615 130 75 5 18 142 16 140 6.59e-18 80.5
MS.gene033668.t1 AT3G04070 30.822 146 91 5 18 156 10 152 8.31e-18 80.1
MS.gene033668.t1 AT2G33480 34.043 141 83 5 5 142 2 135 9.82e-18 79.0
MS.gene033668.t1 AT2G33480 34.043 141 83 5 5 142 2 135 1.04e-17 79.0
MS.gene033668.t1 AT3G04070 30.822 146 91 5 18 156 10 152 1.35e-17 79.7
MS.gene033668.t1 AT5G07680 30.481 187 119 9 13 193 12 193 2.20e-17 79.0
MS.gene033668.t1 AT5G07680 30.978 184 116 9 16 193 1 179 2.52e-17 78.6
MS.gene033668.t1 AT1G01720 29.744 195 115 8 16 194 5 193 2.77e-17 78.2
MS.gene033668.t1 AT5G14000 27.746 173 115 5 5 173 2 168 3.78e-17 76.3
MS.gene033668.t1 AT5G08790 30.496 141 90 5 16 153 5 140 3.84e-17 77.8
MS.gene033668.t1 AT1G77450 32.593 135 83 4 16 147 8 137 4.19e-17 77.0
MS.gene033668.t1 AT2G43000 29.381 194 114 9 13 191 14 199 4.22e-17 77.4
MS.gene033668.t1 AT1G26870 35.556 135 78 7 12 142 12 141 6.39e-17 78.2
MS.gene033668.t1 AT5G18270 35.338 133 80 5 13 142 16 145 7.37e-17 77.4
MS.gene033668.t1 AT5G63790 31.111 135 85 5 16 147 58 187 9.99e-17 77.0
MS.gene033668.t1 AT5G63790 31.111 135 85 5 16 147 48 177 1.03e-16 77.0
MS.gene033668.t1 AT3G10490 36.434 129 75 6 18 142 27 152 1.22e-16 75.5
MS.gene033668.t1 AT5G18270 34.586 133 81 5 13 142 16 145 2.03e-16 76.3
MS.gene033668.t1 AT3G15170 32.593 135 84 6 12 142 14 145 2.42e-16 75.9
MS.gene033668.t1 AT5G53950 33.083 133 82 6 14 142 13 142 3.60e-16 75.9
MS.gene033668.t1 AT3G10480 36.434 129 75 6 18 142 27 152 3.95e-16 76.3
MS.gene033668.t1 AT3G10480 36.434 129 75 6 18 142 27 152 4.19e-16 75.9
MS.gene033668.t1 AT5G61430 30.058 173 112 8 13 180 11 179 4.62e-16 75.5
MS.gene033668.t1 AT2G27300 32.394 142 89 7 6 144 3 140 5.37e-16 75.1
MS.gene033668.t1 AT1G34190 33.858 127 79 4 21 144 19 143 9.32e-16 75.1
MS.gene033668.t1 AT1G34180 33.071 127 80 4 21 144 19 143 9.45e-16 75.1
MS.gene033668.t1 AT5G14000 28.387 155 101 5 5 155 2 150 1.17e-15 71.2
MS.gene033668.t1 AT3G10490 36.434 129 75 6 18 142 27 152 1.23e-15 74.7
MS.gene033668.t1 AT3G29035 33.077 130 80 6 16 142 22 147 1.47e-15 73.9
MS.gene033668.t1 AT3G15510 33.600 125 76 5 22 142 21 142 1.57e-15 73.9
MS.gene033668.t1 AT1G34180 33.071 127 80 4 21 144 19 143 1.71e-15 74.3
MS.gene033668.t1 AT5G39610 32.414 145 83 7 1 142 11 143 2.33e-15 72.8
MS.gene033668.t1 AT5G46590 30.469 128 85 3 18 142 6 132 3.33e-15 72.4
MS.gene033668.t1 AT5G46590 30.469 128 85 3 18 142 6 132 3.58e-15 72.4
MS.gene033668.t1 AT5G66300 32.558 129 81 5 18 142 12 138 4.78e-15 72.0
MS.gene033668.t1 AT4G17980 28.105 153 104 5 18 165 6 157 6.15e-15 71.2
MS.gene033668.t1 AT1G69490 32.031 128 80 5 18 142 9 132 6.58e-15 71.2
MS.gene033668.t1 AT3G04060 33.077 130 81 5 16 142 18 144 9.12e-15 71.6
MS.gene033668.t1 AT4G35580 28.643 199 114 7 18 192 9 203 1.11e-14 72.0
MS.gene033668.t1 AT4G35580 28.643 199 114 7 18 192 9 203 1.28e-14 71.6
MS.gene033668.t1 AT4G35580 28.643 199 114 7 18 192 9 203 1.36e-14 71.6
MS.gene033668.t1 AT4G17980 28.105 153 104 5 18 165 6 157 1.54e-14 70.9
MS.gene033668.t1 AT1G79580 30.534 131 86 4 16 142 15 144 2.32e-14 70.9
MS.gene033668.t1 AT1G79580 30.534 131 86 4 16 142 15 144 2.32e-14 70.9
MS.gene033668.t1 AT1G79580 30.534 131 86 4 16 142 15 144 2.32e-14 70.9
MS.gene033668.t1 AT1G79580 30.534 131 86 4 16 142 15 144 2.32e-14 70.9
MS.gene033668.t1 AT1G79580 30.534 131 86 4 16 142 15 144 2.32e-14 70.9
MS.gene033668.t1 AT5G24590 27.174 184 111 5 18 181 13 193 4.30e-14 70.1
MS.gene033668.t1 AT3G12977 31.884 138 86 6 18 151 18 151 4.86e-14 69.3
MS.gene033668.t1 AT4G27410 29.167 168 109 7 16 178 12 174 5.56e-14 69.3
MS.gene033668.t1 AT5G62380 26.786 168 117 5 12 175 1 166 6.41e-14 69.3
MS.gene033668.t1 AT5G62380 26.786 168 117 5 12 175 1 166 6.41e-14 69.3
MS.gene033668.t1 AT1G12260 28.387 155 105 5 18 168 7 159 7.07e-14 69.3
MS.gene033668.t1 AT1G33060 32.624 141 90 5 5 142 11 149 7.40e-14 69.7
MS.gene033668.t1 AT1G33060 32.624 141 90 5 5 142 11 149 7.41e-14 69.7
MS.gene033668.t1 AT1G12260 28.387 155 105 5 18 168 7 159 1.19e-13 68.9
MS.gene033668.t1 AT1G62700 27.329 161 111 5 17 173 6 164 1.68e-13 68.6
MS.gene033668.t1 AT1G62700 27.329 161 111 5 17 173 6 164 1.68e-13 68.6
MS.gene033668.t1 AT1G52890 31.538 130 82 5 16 142 12 137 2.21e-13 67.8
MS.gene033668.t1 AT4G36160 29.630 135 89 5 12 142 4 136 2.23e-13 67.8
MS.gene033668.t1 AT4G36160 29.630 135 89 5 12 142 4 136 2.23e-13 67.8
MS.gene033668.t1 AT4G36160 29.630 135 89 5 12 142 8 140 2.26e-13 67.8
MS.gene033668.t1 AT1G02230 30.714 140 86 4 16 148 1 136 2.53e-13 67.8
MS.gene033668.t1 AT1G34180 30.216 139 80 5 21 144 19 155 2.67e-13 68.2
MS.gene033668.t1 AT3G18400 32.812 128 79 6 18 142 5 128 3.65e-13 67.0
MS.gene033668.t1 AT1G34180 30.216 139 80 5 21 144 19 155 4.55e-13 67.4
MS.gene033668.t1 AT3G15500 30.769 130 83 4 16 142 12 137 5.83e-13 66.6
MS.gene033668.t1 AT5G09330 33.333 129 81 5 17 142 5 131 5.87e-13 67.0
MS.gene033668.t1 AT5G09330 33.333 129 81 5 17 142 5 131 5.87e-13 67.0
MS.gene033668.t1 AT5G09330 33.333 129 81 5 17 142 5 131 5.87e-13 67.0
MS.gene033668.t1 AT5G09330 33.333 129 81 5 17 142 5 131 5.87e-13 67.0
MS.gene033668.t1 AT5G09330 33.333 129 81 5 17 142 5 131 5.87e-13 67.0
MS.gene033668.t1 AT1G71930 31.008 129 83 5 18 142 9 135 6.29e-13 66.2
MS.gene033668.t1 AT1G71930 31.008 129 83 5 18 142 9 135 6.29e-13 66.2
MS.gene033668.t1 AT2G18060 28.571 133 89 5 14 142 5 135 1.85e-12 65.1
MS.gene033668.t1 AT2G18060 28.571 133 89 5 14 142 5 135 1.85e-12 65.1
MS.gene033668.t1 AT1G32510 30.216 139 83 5 15 142 3 138 3.38e-12 63.9
MS.gene033668.t1 AT4G28530 30.405 148 80 6 15 142 7 151 7.99e-12 63.5
MS.gene033668.t1 AT4G27410 26.230 183 112 7 16 178 12 191 1.29e-11 62.8
MS.gene033668.t1 AT4G01540 27.957 186 121 9 16 193 2 182 1.43e-11 62.8
MS.gene033668.t1 AT4G01540 27.419 186 122 9 16 193 2 182 1.70e-11 62.8
MS.gene033668.t1 AT4G01540 27.419 186 122 9 16 193 2 182 1.73e-11 62.8
MS.gene033668.t1 AT4G01540 27.957 186 121 9 16 193 2 182 1.77e-11 62.4
MS.gene033668.t1 AT3G49530 28.906 128 87 3 18 142 13 139 2.12e-11 62.4
MS.gene033668.t1 AT1G33280 31.298 131 84 5 18 144 8 136 2.31e-11 62.0
MS.gene033668.t1 AT3G49530 28.906 128 87 3 18 142 13 139 3.07e-11 62.0
MS.gene033668.t1 AT4G10350 31.298 131 84 5 18 144 9 137 3.46e-11 61.6
MS.gene033668.t1 AT1G32770 29.197 137 91 5 10 142 8 142 6.75e-11 60.8
MS.gene033668.t1 AT5G64530 30.534 131 76 6 16 144 1 118 7.18e-11 58.5
MS.gene033668.t1 AT1G32870 28.070 171 110 6 1 165 30 193 7.44e-11 60.8
MS.gene033668.t1 AT1G32870 28.834 163 103 6 9 165 2 157 9.16e-11 60.5

Find 46 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAATTACCTGTTGGATTC+AGG 0.250804 7.1:+21718069 MS.gene033668:CDS
GAAAGAACACATTTGTTTAC+TGG 0.255170 7.1:+21718564 MS.gene033668:CDS
GGTACTATTTCTTTGATATC+AGG 0.295817 7.1:+21718435 MS.gene033668:CDS
GAACCGTAGGTTCGAAAATA+TGG 0.299363 7.1:+21718457 MS.gene033668:CDS
AAAGCATAGTGAGCTAGTTT+TGG 0.338409 7.1:+21718037 MS.gene033668:CDS
TCGAATTCTGACAGGACATT+TGG 0.373254 7.1:-21718158 None:intergenic
TGAGTTCATGGAGAGGGAGG+AGG 0.476558 7.1:-21719013 None:intergenic
GGATCAAACCTGAATCCAAC+AGG 0.478056 7.1:-21718077 None:intergenic
AAATCAATCACTGTTGGTGT+GGG 0.492009 7.1:-21718962 None:intergenic
ACAGGACATTTGGAAGAGGT+TGG 0.514459 7.1:-21718148 None:intergenic
GAGTTCATGGAGAGGGAGGA+GGG 0.516664 7.1:-21719012 None:intergenic
AATATGGATGCAAGAAGAGC+TGG 0.518416 7.1:+21718473 MS.gene033668:CDS
GAGCCATGGAAGCTGCCAAA+AGG 0.533677 7.1:+21718194 MS.gene033668:CDS
GAAATCAATCACTGTTGGTG+TGG 0.538024 7.1:-21718963 None:intergenic
GAAGAGCTGGAAATGGTGAA+TGG 0.539470 7.1:+21718486 MS.gene033668:CDS
AGTTCATGGAGAGGGAGGAG+GGG 0.541559 7.1:-21719011 None:intergenic
TTTGTTTACTGGAGAATGCA+AGG 0.541628 7.1:+21718575 MS.gene033668:CDS
CTATCGGATATTTAAGATGA+AGG 0.543622 7.1:+21718916 MS.gene033668:CDS
TCTACAAGCTCATGATCAGT+TGG 0.547810 7.1:-21718098 None:intergenic
GAAATACATCGAATTCTGAC+AGG 0.551455 7.1:-21718166 None:intergenic
GGACCTCTTCAAAGCATATG+AGG 0.554252 7.1:+21718414 MS.gene033668:CDS
TCTGACAGGACATTTGGAAG+AGG 0.561916 7.1:-21718152 None:intergenic
ATGTATTTCAAACTGAGCCA+TGG 0.561975 7.1:+21718180 MS.gene033668:CDS
TGAAACATGAGTTCATGGAG+AGG 0.563520 7.1:-21719020 None:intergenic
GATGGTATGAAATTACCTGT+TGG 0.565216 7.1:+21718062 MS.gene033668:CDS
GATGCAAGAAGAGCTGGAAA+TGG 0.566075 7.1:+21718479 MS.gene033668:CDS
ATCAATCACTGTTGGTGTGG+GGG 0.572846 7.1:-21718960 None:intergenic
ATGTAGGTGTACTCAGCTGT+AGG 0.589112 7.1:+21718890 MS.gene033668:intron
ACTCAGCTGTAGGTGCCTAT+CGG 0.590616 7.1:+21718900 MS.gene033668:CDS
GTACCTCATATGCTTTGAAG+AGG 0.594155 7.1:-21718417 None:intergenic
TCTTTGATATCAGGAACCGT+AGG 0.597083 7.1:+21718444 MS.gene033668:CDS
AGAGACACTTGCATCTTCCA+TGG 0.599268 7.1:-21718987 None:intergenic
CTTCATCTTAAATATCCGAT+AGG 0.611455 7.1:-21718915 None:intergenic
ACATGAGTTCATGGAGAGGG+AGG 0.614894 7.1:-21719016 None:intergenic
GTGAATGGAGAATATATGAG+AGG 0.620604 7.1:+21718501 MS.gene033668:CDS
GAAACATGAGTTCATGGAGA+GGG 0.632009 7.1:-21719019 None:intergenic
AATCAATCACTGTTGGTGTG+GGG 0.641402 7.1:-21718961 None:intergenic
TTGTAGATTATTATCTGACG+AGG 0.656467 7.1:+21718114 MS.gene033668:CDS
AGGATGCATGAATTTGTCAT+CGG 0.657194 7.1:+21718626 MS.gene033668:CDS
GTCAAGCTCTGATGACACAA+TGG 0.657620 7.1:+21718603 MS.gene033668:CDS
ATGGTGAAATCAATCACTGT+TGG 0.663372 7.1:-21718968 None:intergenic
TCGGATATTTAAGATGAAGG+TGG 0.674233 7.1:+21718919 MS.gene033668:CDS
AACAGTGATTGATTTCACCA+TGG 0.680653 7.1:+21718970 MS.gene033668:CDS
GAGAATATATGAGAGGAACG+AGG 0.682363 7.1:+21718508 MS.gene033668:CDS
AAGCTCTGATGACACAATGG+AGG 0.685488 7.1:+21718606 MS.gene033668:CDS
GTTTACTGGAGAATGCAAGG+AGG 0.781160 7.1:+21718578 MS.gene033668:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATAAGAAAAAGAATGAA+GGG - chr7.1:21718235-21718254 None:intergenic 20.0%
!! CCATCATAAGATTAATTAAA+GGG - chr7.1:21718328-21718347 None:intergenic 20.0%
!! TCCATCATAAGATTAATTAA+AGG - chr7.1:21718329-21718348 None:intergenic 20.0%
!!! AACACAAGGAAAATTTTAAA+AGG - chr7.1:21718297-21718316 None:intergenic 20.0%
!!! ACACAAGGAAAATTTTAAAA+GGG - chr7.1:21718296-21718315 None:intergenic 20.0%
!!! TACAATTTTTCATCAAACTA+AGG + chr7.1:21718649-21718668 MS.gene033668:CDS 20.0%
!!! TTTGAAGTAATAATGTGTTT+TGG + chr7.1:21718759-21718778 MS.gene033668:intron 20.0%
!!! TTTTGAACTCAAATTAATGT+AGG + chr7.1:21718874-21718893 MS.gene033668:intron 20.0%
! CCCTTTAATTAATCTTATGA+TGG + chr7.1:21718325-21718344 MS.gene033668:intron 25.0%
! GATCATAAGAAAAAGAATGA+AGG - chr7.1:21718236-21718255 None:intergenic 25.0%
!! CTATAATTTCACAAACCTTT+TGG - chr7.1:21718212-21718231 None:intergenic 25.0%
!!! ATTTGTTTTCTGCAGATAAT+AGG + chr7.1:21718393-21718412 MS.gene033668:intron 25.0%
CTTCATCTTAAATATCCGAT+AGG - chr7.1:21718918-21718937 None:intergenic 30.0%
GAAAGAACACATTTGTTTAC+TGG + chr7.1:21718564-21718583 MS.gene033668:CDS 30.0%
TTGTAGATTATTATCTGACG+AGG + chr7.1:21718114-21718133 MS.gene033668:CDS 30.0%
! CTATCGGATATTTAAGATGA+AGG + chr7.1:21718916-21718935 MS.gene033668:CDS 30.0%
! GGTACTATTTCTTTGATATC+AGG + chr7.1:21718435-21718454 MS.gene033668:CDS 30.0%
!!! TGCTAGTTTTATGCAAAAAG+TGG + chr7.1:21718801-21718820 MS.gene033668:intron 30.0%
AAAGGGTTTCAAAGAACACA+AGG - chr7.1:21718311-21718330 None:intergenic 35.0%
AAATCAATCACTGTTGGTGT+GGG - chr7.1:21718965-21718984 None:intergenic 35.0%
AGGATGCATGAATTTGTCAT+CGG + chr7.1:21718626-21718645 MS.gene033668:CDS 35.0%
ATGGTGAAATCAATCACTGT+TGG - chr7.1:21718971-21718990 None:intergenic 35.0%
ATGTATTTCAAACTGAGCCA+TGG + chr7.1:21718180-21718199 MS.gene033668:CDS 35.0%
GAAATACATCGAATTCTGAC+AGG - chr7.1:21718169-21718188 None:intergenic 35.0%
GATGGTATGAAATTACCTGT+TGG + chr7.1:21718062-21718081 MS.gene033668:CDS 35.0%
GTGAATGGAGAATATATGAG+AGG + chr7.1:21718501-21718520 MS.gene033668:CDS 35.0%
TGAAATTACCTGTTGGATTC+AGG + chr7.1:21718069-21718088 MS.gene033668:CDS 35.0%
TTTGTTTACTGGAGAATGCA+AGG + chr7.1:21718575-21718594 MS.gene033668:CDS 35.0%
! AACAGTGATTGATTTCACCA+TGG + chr7.1:21718970-21718989 MS.gene033668:CDS 35.0%
! CATCCATATTTTCGAACCTA+CGG - chr7.1:21718463-21718482 None:intergenic 35.0%
! TCGGATATTTAAGATGAAGG+TGG + chr7.1:21718919-21718938 MS.gene033668:CDS 35.0%
!! AAAGCATAGTGAGCTAGTTT+TGG + chr7.1:21718037-21718056 MS.gene033668:CDS 35.0%
!! GAAGTATTGCTATCACTATG+AGG + chr7.1:21718730-21718749 MS.gene033668:intron 35.0%
AATATGGATGCAAGAAGAGC+TGG + chr7.1:21718473-21718492 MS.gene033668:CDS 40.0%
AATCAATCACTGTTGGTGTG+GGG - chr7.1:21718964-21718983 None:intergenic 40.0%
GAAATCAATCACTGTTGGTG+TGG - chr7.1:21718966-21718985 None:intergenic 40.0%
GAACCGTAGGTTCGAAAATA+TGG + chr7.1:21718457-21718476 MS.gene033668:CDS 40.0%
GAGAATATATGAGAGGAACG+AGG + chr7.1:21718508-21718527 MS.gene033668:CDS 40.0%
TCGAATTCTGACAGGACATT+TGG - chr7.1:21718161-21718180 None:intergenic 40.0%
TCTACAAGCTCATGATCAGT+TGG - chr7.1:21718101-21718120 None:intergenic 40.0%
TCTTTGATATCAGGAACCGT+AGG + chr7.1:21718444-21718463 MS.gene033668:CDS 40.0%
!! GTACCTCATATGCTTTGAAG+AGG - chr7.1:21718420-21718439 None:intergenic 40.0%
!!! AGTGAGCTAGTTTTGGAAGA+TGG + chr7.1:21718044-21718063 MS.gene033668:CDS 40.0%
ACAGGACATTTGGAAGAGGT+TGG - chr7.1:21718151-21718170 None:intergenic 45.0%
AGAGACACTTGCATCTTCCA+TGG - chr7.1:21718990-21719009 None:intergenic 45.0%
ATCAATCACTGTTGGTGTGG+GGG - chr7.1:21718963-21718982 None:intergenic 45.0%
ATGTAGGTGTACTCAGCTGT+AGG + chr7.1:21718890-21718909 MS.gene033668:intron 45.0%
GAAGAGCTGGAAATGGTGAA+TGG + chr7.1:21718486-21718505 MS.gene033668:CDS 45.0%
GATGCAAGAAGAGCTGGAAA+TGG + chr7.1:21718479-21718498 MS.gene033668:CDS 45.0%
GGACCTCTTCAAAGCATATG+AGG + chr7.1:21718414-21718433 MS.gene033668:CDS 45.0%
GGATCAAACCTGAATCCAAC+AGG - chr7.1:21718080-21718099 None:intergenic 45.0%
GTTTACTGGAGAATGCAAGG+AGG + chr7.1:21718578-21718597 MS.gene033668:CDS 45.0%
TCTGACAGGACATTTGGAAG+AGG - chr7.1:21718155-21718174 None:intergenic 45.0%
! AAACCTTTTGGCAGCTTCCA+TGG - chr7.1:21718200-21718219 None:intergenic 45.0%
! AAGCTCTGATGACACAATGG+AGG + chr7.1:21718606-21718625 MS.gene033668:CDS 45.0%
! GTCAAGCTCTGATGACACAA+TGG + chr7.1:21718603-21718622 MS.gene033668:CDS 45.0%
ACTCAGCTGTAGGTGCCTAT+CGG + chr7.1:21718900-21718919 MS.gene033668:CDS 50.0%
GAGCCATGGAAGCTGCCAAA+AGG + chr7.1:21718194-21718213 MS.gene033668:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 21718023 21719030 21718023 ID=MS.gene033668
chr7.1 mRNA 21718023 21719030 21718023 ID=MS.gene033668.t1;Parent=MS.gene033668
chr7.1 exon 21718023 21718215 21718023 ID=MS.gene033668.t1.exon1;Parent=MS.gene033668.t1
chr7.1 CDS 21718023 21718215 21718023 ID=cds.MS.gene033668.t1;Parent=MS.gene033668.t1
chr7.1 exon 21718408 21718670 21718408 ID=MS.gene033668.t1.exon2;Parent=MS.gene033668.t1
chr7.1 CDS 21718408 21718670 21718408 ID=cds.MS.gene033668.t1;Parent=MS.gene033668.t1
chr7.1 exon 21718896 21719030 21718896 ID=MS.gene033668.t1.exon3;Parent=MS.gene033668.t1
chr7.1 CDS 21718896 21719030 21718896 ID=cds.MS.gene033668.t1;Parent=MS.gene033668.t1
Gene Sequence

>MS.gene033668

ATGGCTGCTGATGAAAAGCATAGTGAGCTAGTTTTGGAAGATGGTATGAAATTACCTGTTGGATTCAGGTTTGATCCAACTGATCATGAGCTTGTAGATTATTATCTGACGAGGAAAGTCTGTAACCAACCTCTTCCAAATGTCCTGTCAGAATTCGATGTATTTCAAACTGAGCCATGGAAGCTGCCAAAAGGTTTGTGAAATTATAGCCCTTCATTCTTTTTCTTATGATCATAGTGTTTTATCAATAAAAATCAAATTTTTACATGACCCTTTTAAAATTTTCCTTGTGTTCTTTGAAACCCTTTAATTAATCTTATGATGGATTTTAATTAATCTTGTGAATGAACATTGTGTTTTAGACAATCTAATTTGTTTTCTGCAGATAATAGGACCTCTTCAAAGCATATGAGGTACTATTTCTTTGATATCAGGAACCGTAGGTTCGAAAATATGGATGCAAGAAGAGCTGGAAATGGTGAATGGAGAATATATGAGAGGAACGAGGAGTTTGCTCTTTCAAACAATCAATTGATTGCGAGAAAGAACACATTTGTTTACTGGAGAATGCAAGGAGGAAGTCAAGCTCTGATGACACAATGGAGGATGCATGAATTTGTCATCGGTACAATTTTTCATCAAACTAAGGTATGCATCAGATGATGCGTAAAATAATCGTATACTGACAAAGTATACTAATTAAACTCGAAGTATTGCTATCACTATGAGGACTTTTTTTGAAGTAATAATGTGTTTTGGTATTTTCAGCTGAAAATCATGCTAGTTTTATGCAAAAAGTGGTTCATTTTTTTGTGTTGTTTGTTTATTAATCAGTTACTATAGTCACTGAATTTTGAACTCAAATTAATGTAGGTGTACTCAGCTGTAGGTGCCTATCGGATATTTAAGATGAAGGTGGCAAAAGCTGACAAGAAAGCCCCCACACCAACAGTGATTGATTTCACCATGGAAGATGCAAGTGTCTCTGCCCCTCCTCCCTCTCCATGA
Protein sequence

>MS.gene033668.t1

MAADEKHSELVLEDGMKLPVGFRFDPTDHELVDYYLTRKVCNQPLPNVLSEFDVFQTEPWKLPKDNRTSSKHMRYYFFDIRNRRFENMDARRAGNGEWRIYERNEEFALSNNQLIARKNTFVYWRMQGGSQALMTQWRMHEFVIGTIFHQTKVYSAVGAYRIFKMKVAKADKKAPTPTVIDFTMEDASVSAPPPSP