Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033673.t1 | RHN47319.1 | 80.4 | 56 | 11 | 0 | 1 | 56 | 1 | 56 | 1.70E-17 | 98.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033673.t1 | Q52QH4 | 45.0 | 60 | 32 | 1 | 4 | 62 | 15 | 74 | 6.2e-08 | 57.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033673.t1 | A0A072U2W3 | 80.4 | 56 | 11 | 0 | 1 | 56 | 1 | 56 | 1.2e-17 | 98.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene033673.t1 | TF | NAC |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033673.t1 | MTR_7g083370 | 80.357 | 56 | 11 | 0 | 1 | 56 | 1 | 56 | 3.15e-27 | 96.3 |
| MS.gene033673.t1 | MTR_7g083330 | 60.938 | 64 | 25 | 0 | 3 | 66 | 11 | 74 | 1.67e-22 | 85.5 |
| MS.gene033673.t1 | MTR_7g083360 | 69.091 | 55 | 17 | 0 | 1 | 55 | 1 | 55 | 2.15e-22 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene033673.t1 | AT5G13180 | 50.000 | 54 | 26 | 1 | 3 | 55 | 7 | 60 | 6.99e-11 | 55.8 |
Find 18 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACCGTAGATTCGAGAATA+TGG | 0.360249 | 7.1:+21686841 | MS.gene033673:CDS |
| GGATCAAACTTGTATCCAAC+AGG | 0.462546 | 7.1:-21686296 | None:intergenic |
| GCTGAGCTTCTTCTACCTGT+TGG | 0.469612 | 7.1:+21686281 | MS.gene033673:CDS |
| AAACCTCTTGGCAGCCTCCA+TGG | 0.486538 | 7.1:-21686416 | None:intergenic |
| GAAGAGCTGGAGATGGTGAA+TGG | 0.497231 | 7.1:+21686870 | MS.gene033673:CDS |
| CATCCATATTCTCGAATCTA+CGG | 0.497709 | 7.1:-21686844 | None:intergenic |
| TCGAATTCAAACAAGTGAGT+TGG | 0.514689 | 7.1:-21686377 | None:intergenic |
| AATATGGATGCAAGAAGAGC+TGG | 0.523452 | 7.1:+21686857 | MS.gene033673:CDS |
| CTTGTTGAGTACTACCTGAC+GGG | 0.535841 | 7.1:+21686332 | MS.gene033673:CDS |
| TCAACAAGCTCGTGATCAGT+TGG | 0.555838 | 7.1:-21686317 | None:intergenic |
| TCAAACAAGTGAGTTGGAAG+AGG | 0.558737 | 7.1:-21686371 | None:intergenic |
| ATGTGTTTCAAACTGAGCCA+TGG | 0.569635 | 7.1:+21686399 | MS.gene033673:CDS |
| GCTTGTTGAGTACTACCTGA+CGG | 0.620029 | 7.1:+21686331 | MS.gene033673:CDS |
| GATGCAAGAAGAGCTGGAGA+TGG | 0.639988 | 7.1:+21686863 | MS.gene033673:CDS |
| ACAAGTGAGTTGGAAGAGGT+AGG | 0.647632 | 7.1:-21686367 | None:intergenic |
| GAGCCATGGAGGCTGCCAAG+AGG | 0.670912 | 7.1:+21686413 | MS.gene033673:CDS |
| TTGTTGAGTACTACCTGACG+GGG | 0.672691 | 7.1:+21686333 | MS.gene033673:CDS |
| TGTTTCAAACTGAGCCATGG+AGG | 0.716472 | 7.1:+21686402 | MS.gene033673:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACAATTTGATTTATTCATTT+TGG | + | chr7.1:21686736-21686755 | MS.gene033673:intron | 15.0% |
| !!! | CAATTTGATTTATTCATTTT+GGG | + | chr7.1:21686737-21686756 | MS.gene033673:intron | 15.0% |
| !! | AACATCATATAAACATTTCA+TGG | - | chr7.1:21686698-21686717 | None:intergenic | 20.0% |
| !! | ACATCATATAAACATTTCAT+GGG | - | chr7.1:21686697-21686716 | None:intergenic | 20.0% |
| !! | CCATCAAAAGATTAATTAAA+TGG | - | chr7.1:21686586-21686605 | None:intergenic | 20.0% |
| !!! | CCATTTAATTAATCTTTTGA+TGG | + | chr7.1:21686583-21686602 | MS.gene033673:intron | 20.0% |
| !!! | TTTATATGATGTTTTCATGT+GGG | + | chr7.1:21686705-21686724 | MS.gene033673:intron | 20.0% |
| !!! | TTTTTTTTTTTGCAGATGAT+AGG | + | chr7.1:21686777-21686796 | MS.gene033673:intron | 20.0% |
| ! | TTTCTATTGATAAGACACAA+TGG | - | chr7.1:21686510-21686529 | None:intergenic | 25.0% |
| !! | AGTACTATTTCTTTGATATC+AGG | + | chr7.1:21686819-21686838 | MS.gene033673:intron | 25.0% |
| !! | GTTTATATGATGTTTTCATG+TGG | + | chr7.1:21686704-21686723 | MS.gene033673:intron | 25.0% |
| CATCCATATTCTCGAATCTA+CGG | - | chr7.1:21686847-21686866 | None:intergenic | 35.0% | |
| CTGAAATTTCACAAACCTCT+TGG | - | chr7.1:21686431-21686450 | None:intergenic | 35.0% | |
| TCGAATTCAAACAAGTGAGT+TGG | - | chr7.1:21686380-21686399 | None:intergenic | 35.0% | |
| ! | TGCAGATGATAGGTTTTCTT+TGG | + | chr7.1:21686787-21686806 | MS.gene033673:intron | 35.0% |
| !!! | ATAGGTTTTCTTTGGAGCAT+AGG | + | chr7.1:21686795-21686814 | MS.gene033673:intron | 35.0% |
| AATATGGATGCAAGAAGAGC+TGG | + | chr7.1:21686857-21686876 | MS.gene033673:CDS | 40.0% | |
| ATGTGTTTCAAACTGAGCCA+TGG | + | chr7.1:21686399-21686418 | MS.gene033673:CDS | 40.0% | |
| GAACCGTAGATTCGAGAATA+TGG | + | chr7.1:21686841-21686860 | MS.gene033673:CDS | 40.0% | |
| GGATCAAACTTGTATCCAAC+AGG | - | chr7.1:21686299-21686318 | None:intergenic | 40.0% | |
| TCTTAGACATGCAGTTGGAA+GGG | + | chr7.1:21686636-21686655 | MS.gene033673:intron | 40.0% | |
| ! | TCAAACAAGTGAGTTGGAAG+AGG | - | chr7.1:21686374-21686393 | None:intergenic | 40.0% |
| CTTGTTGAGTACTACCTGAC+GGG | + | chr7.1:21686332-21686351 | MS.gene033673:CDS | 45.0% | |
| GTCTTAGACATGCAGTTGGA+AGG | + | chr7.1:21686635-21686654 | MS.gene033673:intron | 45.0% | |
| GTGTGTCTTAGACATGCAGT+TGG | + | chr7.1:21686631-21686650 | MS.gene033673:intron | 45.0% | |
| TCAACAAGCTCGTGATCAGT+TGG | - | chr7.1:21686320-21686339 | None:intergenic | 45.0% | |
| TGTTTCAAACTGAGCCATGG+AGG | + | chr7.1:21686402-21686421 | MS.gene033673:CDS | 45.0% | |
| TTGTTGAGTACTACCTGACG+GGG | + | chr7.1:21686333-21686352 | MS.gene033673:CDS | 45.0% | |
| ! | ACAAGTGAGTTGGAAGAGGT+AGG | - | chr7.1:21686370-21686389 | None:intergenic | 45.0% |
| ! | GCTTGTTGAGTACTACCTGA+CGG | + | chr7.1:21686331-21686350 | MS.gene033673:CDS | 45.0% |
| !!! | ACAAAAATTTAAAATTTAAA+TGG | - | chr7.1:21686550-21686569 | None:intergenic | 5.0% |
| GAAGAGCTGGAGATGGTGAA+TGG | + | chr7.1:21686870-21686889 | MS.gene033673:CDS | 50.0% | |
| GATGCAAGAAGAGCTGGAGA+TGG | + | chr7.1:21686863-21686882 | MS.gene033673:CDS | 50.0% | |
| GCTGAGCTTCTTCTACCTGT+TGG | + | chr7.1:21686281-21686300 | MS.gene033673:CDS | 50.0% | |
| TAGACATGCAGTTGGAAGGG+AGG | + | chr7.1:21686639-21686658 | MS.gene033673:intron | 50.0% | |
| ! | GGTTGAGAATTTTCCCCGTC+AGG | - | chr7.1:21686349-21686368 | None:intergenic | 50.0% |
| AAACCTCTTGGCAGCCTCCA+TGG | - | chr7.1:21686419-21686438 | None:intergenic | 55.0% | |
| GAGCCATGGAGGCTGCCAAG+AGG | + | chr7.1:21686413-21686432 | MS.gene033673:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 21686266 | 21686899 | 21686266 | ID=MS.gene033673 |
| chr7.1 | mRNA | 21686266 | 21686899 | 21686266 | ID=MS.gene033673.t1;Parent=MS.gene033673 |
| chr7.1 | exon | 21686266 | 21686434 | 21686266 | ID=MS.gene033673.t1.exon1;Parent=MS.gene033673.t1 |
| chr7.1 | CDS | 21686266 | 21686434 | 21686266 | ID=cds.MS.gene033673.t1;Parent=MS.gene033673.t1 |
| chr7.1 | exon | 21686841 | 21686899 | 21686841 | ID=MS.gene033673.t1.exon2;Parent=MS.gene033673.t1 |
| chr7.1 | CDS | 21686841 | 21686899 | 21686841 | ID=cds.MS.gene033673.t1;Parent=MS.gene033673.t1 |
| Gene Sequence |
| Protein sequence |
