AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033704

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033704.t1 MTR_7g083900 97.113 485 3 6 1 484 1 475 0.0 942
MS.gene033704.t1 MTR_7g083900 96.914 486 3 7 1 484 1 476 0.0 938
MS.gene033704.t1 MTR_3g498695 46.809 517 177 17 21 483 12 484 2.00e-123 369
MS.gene033704.t1 MTR_3g498695 46.615 517 177 18 21 483 12 483 6.01e-121 363
MS.gene033704.t1 MTR_3g030700 50.368 272 114 5 222 481 113 375 3.93e-72 233
MS.gene033704.t1 MTR_2g081040 57.143 189 79 1 293 481 175 361 1.03e-66 219
MS.gene033704.t1 MTR_3g104100 54.187 203 91 1 279 481 179 379 8.79e-64 211
MS.gene033704.t1 MTR_3g111290 44.898 196 80 5 288 482 166 334 7.74e-39 144
MS.gene033704.t1 MTR_5g030770 44.878 205 85 5 283 482 141 322 3.57e-37 139
MS.gene033704.t1 MTR_5g030770 44.390 205 80 5 283 482 141 316 3.10e-35 134
MS.gene033704.t1 MTR_1g112680 32.653 245 130 6 250 476 286 513 2.03e-30 124
MS.gene033704.t1 MTR_8g064700 29.787 188 123 3 288 475 237 415 1.42e-18 88.2
MS.gene033704.t1 MTR_2g038040 33.508 191 114 6 291 474 48 232 6.80e-18 83.6
MS.gene033704.t1 MTR_1g072320 33.690 187 105 6 282 457 450 628 1.73e-17 85.9
MS.gene033704.t1 MTR_5g014600 26.368 201 137 4 285 475 135 334 4.63e-15 76.6
MS.gene033704.t1 MTR_4g009540 28.713 202 124 7 283 475 140 330 6.57e-15 76.3
MS.gene033704.t1 MTR_5g014640 29.412 187 110 5 295 475 154 324 1.82e-14 74.7
MS.gene033704.t1 MTR_4g009110 29.000 200 123 7 283 475 162 349 3.00e-14 74.3
MS.gene033704.t1 MTR_4g009540 28.409 176 110 6 283 453 140 304 5.40e-14 73.2
MS.gene033704.t1 MTR_4g057270 51.515 66 32 0 286 351 140 205 9.64e-14 72.8
MS.gene033704.t1 MTR_8g024790 57.895 57 24 0 293 349 289 345 1.06e-13 73.6
MS.gene033704.t1 MTR_2g084280 29.577 142 85 1 294 435 132 258 1.21e-13 72.0
MS.gene033704.t1 MTR_8g024770 60.000 55 22 0 293 347 277 331 1.82e-13 72.8
MS.gene033704.t1 MTR_7g117670 45.977 87 44 1 293 376 400 486 2.54e-13 72.8
MS.gene033704.t1 MTR_2g010450 28.302 159 99 3 297 453 179 324 3.13e-13 71.2
MS.gene033704.t1 MTR_8g027495 53.333 60 28 0 293 352 456 515 3.86e-13 72.0
MS.gene033704.t1 MTR_7g096350 42.045 88 43 1 271 350 323 410 4.54e-13 71.6
MS.gene033704.t1 MTR_4g092700 27.586 203 118 5 275 466 138 322 4.56e-13 70.5
MS.gene033704.t1 MTR_8g067280 33.133 166 96 4 293 451 460 617 5.17e-13 71.6
MS.gene033704.t1 MTR_4g094762 25.743 202 136 5 280 476 278 470 6.16e-13 71.2
MS.gene033704.t1 MTR_5g030430 29.231 195 119 5 293 479 491 674 2.34e-12 69.7
MS.gene033704.t1 MTR_4g097950 26.705 176 115 3 272 438 38 208 1.03e-11 65.5
MS.gene033704.t1 MTR_5g014520 30.189 159 97 4 296 453 158 303 1.30e-11 66.2
MS.gene033704.t1 MTR_4g067010 28.293 205 127 6 271 473 125 311 2.08e-11 65.5
MS.gene033704.t1 MTR_0250s0040 28.649 185 103 5 296 471 89 253 2.26e-11 64.7
MS.gene033704.t1 MTR_8g009020 26.752 157 110 3 297 453 145 296 3.11e-11 65.1
MS.gene033704.t1 MTR_4g097920 28.502 207 114 7 277 466 113 302 3.72e-11 64.7
MS.gene033704.t1 MTR_4g097920 28.502 207 114 7 277 466 128 317 3.86e-11 64.7
MS.gene033704.t1 MTR_5g021250 62.857 105 18 1 53 157 1 84 4.92e-11 60.1
MS.gene033704.t1 MTR_4g066460 26.941 219 140 6 257 473 119 319 6.89e-11 63.9
MS.gene033704.t1 MTR_2g104550 25.743 202 132 4 282 476 122 312 7.08e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033704.t1 AT3G26744 56.041 389 138 10 120 483 113 493 7.89e-118 355
MS.gene033704.t1 AT3G26744 56.041 389 138 10 120 483 113 493 7.89e-118 355
MS.gene033704.t1 AT3G26744 56.041 389 138 10 120 483 113 493 7.89e-118 355
MS.gene033704.t1 AT1G12860 57.377 366 109 10 129 483 120 449 4.17e-112 339
MS.gene033704.t1 AT3G26744 55.096 363 130 10 120 457 113 467 2.11e-101 312
MS.gene033704.t1 AT1G12860 56.119 335 110 10 129 457 120 423 2.17e-95 295
MS.gene033704.t1 AT5G65640 43.689 206 95 4 281 482 160 348 1.16e-36 139
MS.gene033704.t1 AT5G10570 43.655 197 81 4 288 482 144 312 1.43e-32 126
MS.gene033704.t1 AT2G16910 40.102 197 99 6 294 476 313 504 5.66e-31 126
MS.gene033704.t1 AT5G65640 68.919 74 19 1 281 350 160 233 2.13e-20 91.7
MS.gene033704.t1 AT1G12860 39.901 203 93 8 129 326 120 298 9.26e-19 87.4
MS.gene033704.t1 AT3G26744 38.095 231 112 9 120 325 113 337 8.51e-18 85.5
MS.gene033704.t1 AT5G57150 32.240 183 115 4 293 472 52 228 1.23e-17 82.8
MS.gene033704.t1 AT4G29930 30.323 155 88 3 294 448 53 187 2.40e-16 78.6
MS.gene033704.t1 AT4G29930 30.323 155 88 3 294 448 53 187 7.61e-16 77.4
MS.gene033704.t1 AT5G57150 33.125 160 98 4 293 449 53 206 8.26e-16 77.0
MS.gene033704.t1 AT5G57150 33.333 159 97 4 293 448 53 205 9.30e-16 77.0
MS.gene033704.t1 AT5G57150 33.333 159 97 4 293 448 52 204 1.24e-15 76.6
MS.gene033704.t1 AT2G28160 36.607 112 59 2 240 351 88 187 2.40e-15 77.4
MS.gene033704.t1 AT4G29930 30.323 155 91 4 294 448 53 190 2.67e-15 75.5
MS.gene033704.t1 AT2G28160 36.607 112 59 2 240 351 88 187 2.94e-15 77.0
MS.gene033704.t1 AT5G57150 33.333 159 97 4 293 448 53 205 6.64e-15 75.1
MS.gene033704.t1 AT4G29930 47.761 67 35 0 294 360 53 119 1.64e-14 72.4
MS.gene033704.t1 AT4G09820 33.333 153 67 4 288 437 353 473 2.09e-14 75.9
MS.gene033704.t1 AT2G46510 45.122 82 37 1 277 350 369 450 3.22e-14 75.5
MS.gene033704.t1 AT4G29930 47.761 67 35 0 294 360 53 119 3.49e-14 72.8
MS.gene033704.t1 AT4G29930 47.059 68 36 0 293 360 52 119 5.10e-14 72.4
MS.gene033704.t1 AT4G29930 47.059 68 36 0 293 360 52 119 5.10e-14 72.4
MS.gene033704.t1 AT1G01260 40.984 122 56 3 243 359 392 502 7.94e-14 74.3
MS.gene033704.t1 AT1G01260 40.984 122 56 3 243 359 392 502 7.94e-14 74.3
MS.gene033704.t1 AT1G01260 40.984 122 56 3 243 359 392 502 7.94e-14 74.3
MS.gene033704.t1 AT4G17880 30.159 189 114 6 293 479 414 586 1.60e-13 73.2
MS.gene033704.t1 AT5G46760 31.707 164 94 5 293 451 413 563 3.92e-13 72.0
MS.gene033704.t1 AT1G32640 29.744 195 108 6 293 479 450 623 4.95e-13 72.0
MS.gene033704.t1 AT1G10610 43.678 87 43 2 272 352 138 224 1.36e-12 69.3
MS.gene033704.t1 AT1G10610 43.678 87 43 2 272 352 205 291 2.12e-12 69.3
MS.gene033704.t1 AT4G00870 47.761 67 33 1 288 352 240 306 2.33e-12 69.3
MS.gene033704.t1 AT1G10610 43.678 87 43 2 272 352 235 321 3.09e-12 68.9
MS.gene033704.t1 AT5G46830 31.250 192 96 8 293 470 341 510 5.68e-12 68.2
MS.gene033704.t1 AT2G22760 23.926 163 113 4 292 453 116 268 6.25e-12 66.6
MS.gene033704.t1 AT2G22760 23.926 163 113 4 292 453 116 268 1.78e-11 65.5
MS.gene033704.t1 AT4G21330 29.000 200 114 6 285 476 22 201 3.10e-11 63.2
MS.gene033704.t1 AT4G16430 41.975 81 39 1 278 350 295 375 3.89e-11 65.5
MS.gene033704.t1 AT2G22750 27.273 143 97 2 296 438 151 286 7.82e-11 63.9
MS.gene033704.t1 AT2G22750 27.273 143 97 2 296 438 155 290 8.50e-11 63.9
MS.gene033704.t1 AT2G22750 27.273 143 97 2 296 438 155 290 9.28e-11 63.5

Find 138 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCCATTTCAAATGGGTTAT+TGG 0.117688 7.1:+21127338 None:intergenic
GTTTGAAAATGGATTCTTTA+TGG 0.140965 7.1:-21127316 MS.gene033704:CDS
TTTGAAATGGGGTTTGAAAA+TGG 0.212995 7.1:-21127327 MS.gene033704:CDS
AATCTATGGTGTTTGTGTTT+TGG 0.283895 7.1:+21127424 None:intergenic
TCAGGTTCGGGTTGTGGTTC+GGG 0.284310 7.1:+21127534 None:intergenic
TTTGAAAATGGATTCTTTAT+GGG 0.287664 7.1:-21127315 MS.gene033704:CDS
ATCAGGTTCGGGTTGTGGTT+CGG 0.308632 7.1:+21127533 None:intergenic
TTGTGTTTAGGTTCGGAGTT+AGG 0.321519 7.1:+21127621 None:intergenic
GTTGTTGTTGTTATCAGGTT+CGG 0.325642 7.1:+21127521 None:intergenic
ACCAATAACCCATTTGAAAT+GGG 0.328953 7.1:-21127339 MS.gene033704:CDS
TCAGCCATCAAATTCTTTGC+AGG 0.331079 7.1:+21126856 None:intergenic
TACCAATAACCCATTTGAAA+TGG 0.336052 7.1:-21127340 MS.gene033704:CDS
TGGATTTGAAATTGAATCTA+TGG 0.348162 7.1:+21127410 None:intergenic
AGAGAAAGGTGGAGAAGAGC+TGG 0.362560 7.1:-21127029 MS.gene033704:CDS
GCTATCTACCATGAGGGCTT+TGG 0.366010 7.1:-21125678 MS.gene033704:CDS
TTGTTGTTGTTATCAGGTTC+GGG 0.372267 7.1:+21127522 None:intergenic
TCAAATGGGACTGTTGTTAC+TGG 0.376369 7.1:-21126910 MS.gene033704:CDS
AACAAGAATCACCTTCTTCA+TGG 0.390694 7.1:-21127650 MS.gene033704:CDS
TACAGGTGGAAGTTAGAGTA+AGG 0.391657 7.1:-21125754 MS.gene033704:intron
AGGGACCCACCCATGAAAAC+AGG 0.394342 7.1:+21127270 None:intergenic
TTGTTATCAGGTTCGGGTTG+TGG 0.401185 7.1:+21127528 None:intergenic
TGTGGTTCGGGTTTGAAGAA+AGG 0.402846 7.1:+21127546 None:intergenic
AATGATTTGAATAATTCAAA+TGG 0.403338 7.1:-21126925 MS.gene033704:CDS
CGCTGTTTCAGAAGCGTCGT+GGG 0.407593 7.1:-21127095 MS.gene033704:CDS
GCTGTTATCAGTTGTTTCAA+TGG 0.414184 7.1:-21125629 MS.gene033704:CDS
GTGGTTGTTGTGTTTAGGTT+CGG 0.421630 7.1:+21127614 None:intergenic
TGTTCGTGGTTGTTGTGTTT+AGG 0.423157 7.1:+21127609 None:intergenic
TGATGATGTTGTTGTGGTTG+TGG 0.425371 7.1:+21127153 None:intergenic
ATGAACTTGTTGAGGTGGTA+AGG 0.429477 7.1:+21127713 None:intergenic
TAATAATCTTCATAATGAAC+TGG 0.430336 7.1:-21126125 MS.gene033704:CDS
GAAGGTTGCAGCAGTGAGCC+AGG 0.431678 7.1:+21126091 None:intergenic
GCTTCCTGCAAAGAATTTGA+TGG 0.433280 7.1:-21126860 MS.gene033704:CDS
CAGCACTGCTTTAATTTGCT+CGG 0.447013 7.1:+21125351 None:intergenic
TAGATCCTCTTTGACTCGAC+AGG 0.449537 7.1:+21126021 None:intergenic
GTGGTTCGGGTTTGAAGAAA+GGG 0.450268 7.1:+21127547 None:intergenic
AGCACTGCTTTAATTTGCTC+GGG 0.451779 7.1:+21125352 None:intergenic
GATCAGAAGGGGAAGAAGAA+GGG 0.453237 7.1:-21126883 MS.gene033704:CDS
GAAGAAGGAAGTGGTGGTGG+TGG 0.454840 7.1:-21127003 MS.gene033704:CDS
GCAATAGCTATTGCTATGGC+TGG 0.459874 7.1:-21127579 MS.gene033704:CDS
TATCATTGAGTTTCTTCCTC+CGG 0.461791 7.1:+21126827 None:intergenic
CCTTGGATTGGATGTTCAGC+AGG 0.462769 7.1:-21125651 MS.gene033704:CDS
GAAGGAAGTGGTGGTGGTGG+TGG 0.463647 7.1:-21127000 MS.gene033704:CDS
ATGATAGGTTGTATATGCTT+AGG 0.465770 7.1:-21126810 MS.gene033704:CDS
GGCTTTGGATAACCTTGGAT+TGG 0.469562 7.1:-21125663 MS.gene033704:CDS
TTCCCCACGGTCTCCAACCT+CGG 0.472317 7.1:+21127060 None:intergenic
GAGAAGAGCTGGGAAGAAGA+AGG 0.472684 7.1:-21127018 MS.gene033704:CDS
AATTATGATTCTGATGAGAA+TGG 0.474232 7.1:-21126949 MS.gene033704:CDS
GGCGTTGGAGATTCCGAGGT+TGG 0.477216 7.1:-21127073 MS.gene033704:CDS
GCAGCAATGCAGAGAAGGCC+AGG 0.479876 7.1:-21125383 MS.gene033704:intron
AGATCAGAAGGGGAAGAAGA+AGG 0.480045 7.1:-21126884 MS.gene033704:CDS
TCGGTGGAGTGGGTGTCAAC+GGG 0.480196 7.1:+21126050 None:intergenic
TTTGCTCGGGGAGTACATCC+TGG 0.485199 7.1:+21125365 None:intergenic
ATGATTTGAATAATTCAAAT+GGG 0.485439 7.1:-21126924 MS.gene033704:CDS
GCAAATTAAAGCAGTGCTGT+TGG 0.486197 7.1:-21125347 MS.gene033704:CDS
CATGAGAAAATTGTCTGGTA+AGG 0.488924 7.1:+21127449 None:intergenic
TTTGGGGCTTAGCAAGTCAC+CGG 0.489029 7.1:+21125994 None:intergenic
TTTCAAACCCCATTTCAAAT+GGG 0.490689 7.1:+21127331 None:intergenic
CTATTGCAATGTCTTGTTCG+TGG 0.492928 7.1:+21127595 None:intergenic
ACAAAACATATGAATGTTGA+CGG 0.497241 7.1:+21125721 None:intergenic
TTGGATTCAGCGGGCTACCA+TGG 0.497757 7.1:-21125328 MS.gene033704:CDS
TCAGAAGCGTCGTGGGGCGT+TGG 0.498023 7.1:-21127088 MS.gene033704:CDS
CGCTTCTGAAACAGCGTCGT+GGG 0.499455 7.1:+21127102 None:intergenic
GTCGGTGGAGTGGGTGTCAA+CGG 0.499586 7.1:+21126049 None:intergenic
ATGAGGGCTTTGGATAACCT+TGG 0.500549 7.1:-21125668 MS.gene033704:CDS
AGGAAACTCAGAAGCTGAAA+GGG 0.502922 7.1:+21127251 None:intergenic
GAAGAAGAAGGAAGTGGTGG+TGG 0.503536 7.1:-21127006 MS.gene033704:CDS
GAGAAAGGTGGAGAAGAGCT+GGG 0.504559 7.1:-21127028 MS.gene033704:CDS
TGTTGTTGTGGTTCAGTCCA+AGG 0.506094 7.1:+21127675 None:intergenic
ATGGACAGGGCTTCAATACT+CGG 0.509966 7.1:-21126187 MS.gene033704:CDS
GAAGCACTGAAAGGTGGTAC+TGG 0.513909 7.1:+21127204 None:intergenic
GGAGGAAGAAACTCAATGAT+AGG 0.514773 7.1:-21126825 MS.gene033704:CDS
AGCTGGGAAGAAGAAGGAAG+TGG 0.518800 7.1:-21127012 MS.gene033704:CDS
TCTCTTCTTCTTGTTCCCCA+CGG 0.521728 7.1:+21127047 None:intergenic
TGGTGCATGAGAAAATTGTC+TGG 0.522663 7.1:+21127444 None:intergenic
ATTATGATTCTGATGAGAAT+GGG 0.525864 7.1:-21126948 MS.gene033704:CDS
ATTTGATGGCTGAGAGGCGC+CGG 0.526012 7.1:-21126846 MS.gene033704:CDS
CAGGGAAGAGTCGGTGGAGT+GGG 0.527264 7.1:+21126040 None:intergenic
GTCAAAGAGGATCTATATCC+CGG 0.532043 7.1:-21126013 MS.gene033704:CDS
GAGGAAACTCAGAAGCTGAA+AGG 0.532923 7.1:+21127250 None:intergenic
TGAACTTGTTGAGGTGGTAA+GGG 0.534290 7.1:+21127714 None:intergenic
ATAATACTCTCACTATACCT+TGG 0.539865 7.1:-21127692 MS.gene033704:CDS
ATCAGAAGGGGAAGAAGAAG+GGG 0.540168 7.1:-21126882 MS.gene033704:CDS
ACAGGTGGAAGTTAGAGTAA+GGG 0.540261 7.1:-21125753 MS.gene033704:intron
AGCAGTGCTGTTGGATTCAG+CGG 0.541017 7.1:-21125338 MS.gene033704:CDS
GTGGAAGTTAGAGTAAGGGA+AGG 0.547671 7.1:-21125749 MS.gene033704:CDS
TCTTAGCAGCAATGCAGAGA+AGG 0.548391 7.1:-21125388 MS.gene033704:intron
GATTCCGAGGTTGGAGACCG+TGG 0.550901 7.1:-21127064 MS.gene033704:CDS
CCAATAACCCATTTGAAATG+GGG 0.552722 7.1:-21127338 MS.gene033704:CDS
AGATGCAGTTGACTACTTGA+AGG 0.553444 7.1:-21126164 MS.gene033704:CDS
ACGCTGTTTCAGAAGCGTCG+TGG 0.560372 7.1:-21127096 MS.gene033704:CDS
TGAAAGCTTGCACTTGCAGA+AGG 0.560535 7.1:+21126073 None:intergenic
CATTGCAATAGCTATTGCTA+TGG 0.561250 7.1:-21127583 MS.gene033704:CDS
AAGGTTATCCAAAGCCCTCA+TGG 0.562752 7.1:+21125670 None:intergenic
TTTAGGTTCGGAGTTAGGTG+TGG 0.567779 7.1:+21127626 None:intergenic
TGATGATGATGATGTTGTTG+TGG 0.568352 7.1:+21127147 None:intergenic
ATTCCGAGGTTGGAGACCGT+GGG 0.568857 7.1:-21127063 MS.gene033704:CDS
TGTTACTGGTGGAGATCAGA+AGG 0.571281 7.1:-21126896 MS.gene033704:CDS
TGGAAGTTAGAGTAAGGGAA+GGG 0.572558 7.1:-21125748 MS.gene033704:CDS
TGTGGTTGAGCTAGCTCAAG+AGG 0.578961 7.1:+21127171 None:intergenic
ATGAGAAAATTGTCTGGTAA+GGG 0.579342 7.1:+21127450 None:intergenic
GAACAAGAAGAAGAGAAAGG+TGG 0.581110 7.1:-21127040 MS.gene033704:CDS
TGGGAAGAAGAAGGAAGTGG+TGG 0.581705 7.1:-21127009 MS.gene033704:CDS
TTAGGTGTGGACCATGAAGA+AGG 0.581820 7.1:+21127639 None:intergenic
GTATTATGAACTTGTTGAGG+TGG 0.581897 7.1:+21127708 None:intergenic
TTGTTGTGAAAGAAAGAGTG+TGG 0.583509 7.1:+21127390 None:intergenic
TCTTCCCTGTCGAGTCAAAG+AGG 0.586826 7.1:-21126026 MS.gene033704:CDS
GGGACCCACCCATGAAAACA+GGG 0.591270 7.1:+21127271 None:intergenic
AATGGGACTGTTGTTACTGG+TGG 0.595592 7.1:-21126907 MS.gene033704:CDS
TTGACTCGACAGGGAAGAGT+CGG 0.596564 7.1:+21126031 None:intergenic
TGATGGCTGAGAGGCGCCGG+AGG 0.599553 7.1:-21126843 MS.gene033704:CDS
ACTTGAAGGAGCTGCTGCAG+CGG 0.611585 7.1:-21126150 MS.gene033704:CDS
ACTCGACAGGGAAGAGTCGG+TGG 0.612573 7.1:+21126034 None:intergenic
CAAAGAATTTGATGGCTGAG+AGG 0.613701 7.1:-21126852 MS.gene033704:CDS
ACAGGGAAGAGTCGGTGGAG+TGG 0.614037 7.1:+21126039 None:intergenic
ATGGTAGATAGCAAAAGTCC+TGG 0.618329 7.1:+21125689 None:intergenic
ATAGAAAACGAAGCACTGAA+AGG 0.618637 7.1:+21127195 None:intergenic
GAACTGGAATCAACTCCACC+TGG 0.619030 7.1:-21126109 MS.gene033704:CDS
GGGGAACAAGAAGAAGAGAA+AGG 0.621823 7.1:-21127043 MS.gene033704:CDS
ACTTCATTCAATTCAAACCA+TGG 0.624434 7.1:+21125311 None:intergenic
GTTACTGGTGGAGATCAGAA+GGG 0.630933 7.1:-21126895 MS.gene033704:CDS
ATTAGACGTGTTCAGAGCTG+AGG 0.631196 7.1:-21125600 MS.gene033704:intron
TGTTGTGCCTAAAATTAGCA+AGG 0.631769 7.1:-21126785 MS.gene033704:intron
TTGGGGCTTAGCAAGTCACC+GGG 0.636110 7.1:+21125995 None:intergenic
GTGGGGCGTTGGAGATTCCG+AGG 0.636781 7.1:-21127077 MS.gene033704:CDS
GAAAACGAAGCACTGAAAGG+TGG 0.640228 7.1:+21127198 None:intergenic
CCTGCTGAACATCCAATCCA+AGG 0.640256 7.1:+21125651 None:intergenic
ACGCTTCTGAAACAGCGTCG+TGG 0.647570 7.1:+21127101 None:intergenic
GCAGTGCTGTTGGATTCAGC+GGG 0.652187 7.1:-21125337 MS.gene033704:CDS
AGATCCTCTTTGACTCGACA+GGG 0.653087 7.1:+21126022 None:intergenic
GCACTGCTTTAATTTGCTCG+GGG 0.667619 7.1:+21125353 None:intergenic
TTACTGGTGGAGATCAGAAG+GGG 0.669179 7.1:-21126894 MS.gene033704:CDS
GGTTGCAGCAGTGAGCCAGG+TGG 0.670958 7.1:+21126094 None:intergenic
GCTGTTTCAGAAGCGTCGTG+GGG 0.673826 7.1:-21127094 MS.gene033704:CDS
AGAGTATTATGAACTTGTTG+AGG 0.689686 7.1:+21127705 None:intergenic
TTCCGAGGTTGGAGACCGTG+GGG 0.692226 7.1:-21127062 MS.gene033704:CDS
GGACCCACCCATGAAAACAG+GGG 0.693712 7.1:+21127272 None:intergenic
GCAGCATCGAGTTCAAGACT+CGG 0.694688 7.1:+21127228 None:intergenic
GCATCGAGTTCAAGACTCGG+AGG 0.717250 7.1:+21127231 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTCATTTATTTTATAGA+TGG - chr7.1:21126844-21126863 MS.gene033704:CDS 10.0%
!!! TAATTTTACTATTTATTCAA+TGG + chr7.1:21126545-21126564 None:intergenic 10.0%
!! AATGATTTGAATAATTCAAA+TGG - chr7.1:21126125-21126144 MS.gene033704:CDS 15.0%
!! ATGATTTGAATAATTCAAAT+GGG - chr7.1:21126126-21126145 MS.gene033704:CDS 15.0%
!! TTTACAAAATATTGTGAAAT+TGG - chr7.1:21126305-21126324 MS.gene033704:intron 15.0%
!!! AAAAATGTTATGTTTTTTGA+AGG + chr7.1:21126577-21126596 None:intergenic 15.0%
!!! CATCAAATATTTTTTCTAAA+AGG + chr7.1:21126333-21126352 None:intergenic 15.0%
!! GATTTCTATTATTCAAATCA+AGG + chr7.1:21126605-21126624 None:intergenic 20.0%
!! TAATAATCTTCATAATGAAC+TGG - chr7.1:21126925-21126944 MS.gene033704:CDS 20.0%
!! TGAATAATAGAAATCATGAA+GGG - chr7.1:21126610-21126629 MS.gene033704:intron 20.0%
!! TTGAATAATAGAAATCATGA+AGG - chr7.1:21126609-21126628 MS.gene033704:intron 20.0%
!!! ACTAGTTTTTTGTATTTTGT+TGG - chr7.1:21126648-21126667 MS.gene033704:intron 20.0%
!!! ATTTATTTTATAGATGGACA+GGG - chr7.1:21126850-21126869 MS.gene033704:CDS 20.0%
!!! TCCATTAATGTTTTAAATTG+CGG - chr7.1:21126369-21126388 MS.gene033704:intron 20.0%
!!! TTGCTTTTGTAATTTTGTTT+GGG - chr7.1:21126710-21126729 MS.gene033704:intron 20.0%
!!! TTTGAAAATGGATTCTTTAT+GGG - chr7.1:21125735-21125754 MS.gene033704:CDS 20.0%
! AAATTACAAAAGCAACAAGA+TGG + chr7.1:21126705-21126724 None:intergenic 25.0%
! AATGTAAGAAAGGTTTATGA+TGG + chr7.1:21127517-21127536 None:intergenic 25.0%
! AATTATGATTCTGATGAGAA+TGG - chr7.1:21126101-21126120 MS.gene033704:CDS 25.0%
! ACAAAACATATGAATGTTGA+CGG + chr7.1:21127332-21127351 None:intergenic 25.0%
! ATTATGATTCTGATGAGAAT+GGG - chr7.1:21126102-21126121 MS.gene033704:CDS 25.0%
! CAAAAAATTGTTGTCAAATG+CGG - chr7.1:21126423-21126442 MS.gene033704:intron 25.0%
! CATACAGTAAAATGTAAGAA+AGG + chr7.1:21127527-21127546 None:intergenic 25.0%
! TCAATGGCATTTCTAATTAT+AGG + chr7.1:21126529-21126548 None:intergenic 25.0%
! TTCACTAATTATTGGATTTC+TGG - chr7.1:21127618-21127637 MS.gene033704:CDS 25.0%
!! AATGTTGTTGCTTATGTTAT+TGG - chr7.1:21126749-21126768 MS.gene033704:intron 25.0%
!! ACCGCAATTTAAAACATTAA+TGG + chr7.1:21126373-21126392 None:intergenic 25.0%
!! GTTTGAAAATGGATTCTTTA+TGG - chr7.1:21125734-21125753 MS.gene033704:CDS 25.0%
!! TGGATTTGAAATTGAATCTA+TGG + chr7.1:21125643-21125662 None:intergenic 25.0%
!!! ATTTCGAGTTCACTAATTAT+TGG - chr7.1:21127610-21127629 MS.gene033704:CDS 25.0%
!!! CATTTATTTTATAGATGGAC+AGG - chr7.1:21126849-21126868 MS.gene033704:CDS 25.0%
!!! GTTGCTTTTGTAATTTTGTT+TGG - chr7.1:21126709-21126728 MS.gene033704:intron 25.0%
!!! TGCTTTTGTAATTTTGTTTG+GGG - chr7.1:21126711-21126730 MS.gene033704:intron 25.0%
!!! TTCTTGTTTCTGAAATTTAC+AGG - chr7.1:21127279-21127298 MS.gene033704:CDS 25.0%
AAATAGAACGTGCTAATGTT+AGG - chr7.1:21126811-21126830 MS.gene033704:CDS 30.0%
AAGGCAAACAAAAATTACCT+TGG + chr7.1:21127092-21127111 None:intergenic 30.0%
AATTATTTGGACGGTAATTG+AGG - chr7.1:21127242-21127261 MS.gene033704:CDS 30.0%
ACATGGCAAATAACTGAATA+AGG - chr7.1:21127206-21127225 MS.gene033704:CDS 30.0%
AGGCAAACAAAAATTACCTT+GGG + chr7.1:21127091-21127110 None:intergenic 30.0%
ATAATACTCTCACTATACCT+TGG - chr7.1:21125358-21125377 MS.gene033704:CDS 30.0%
ATGAGAAAATTGTCTGGTAA+GGG + chr7.1:21125603-21125622 None:intergenic 30.0%
ATGATAGGTTGTATATGCTT+AGG - chr7.1:21126240-21126259 MS.gene033704:intron 30.0%
CACAAAAAACAACCTAAAAG+AGG + chr7.1:21127571-21127590 None:intergenic 30.0%
CATGGCAAATAACTGAATAA+GGG - chr7.1:21127207-21127226 MS.gene033704:CDS 30.0%
CATGTTACTGGTATAGATAA+AGG + chr7.1:21127191-21127210 None:intergenic 30.0%
CCTAAAATTAGCAAGGTATA+TGG - chr7.1:21126272-21126291 MS.gene033704:intron 30.0%
CTTAAAACAACTTCGCATTA+AGG + chr7.1:21127111-21127130 None:intergenic 30.0%
CTTTATCTATACCAGTAACA+TGG - chr7.1:21127189-21127208 MS.gene033704:CDS 30.0%
GTACGTTGATGCAAATTATT+TGG - chr7.1:21127229-21127248 MS.gene033704:CDS 30.0%
GTTGATGCAAATTATTTGGA+CGG - chr7.1:21127233-21127252 MS.gene033704:CDS 30.0%
TACCAATAACCCATTTGAAA+TGG - chr7.1:21125710-21125729 MS.gene033704:CDS 30.0%
TTTCAAACCCCATTTCAAAT+GGG + chr7.1:21125722-21125741 None:intergenic 30.0%
! AAGTTGTTGTTGTTGTTATC+AGG + chr7.1:21125537-21125556 None:intergenic 30.0%
! AATCTATGGTGTTTGTGTTT+TGG + chr7.1:21125629-21125648 None:intergenic 30.0%
! ACCAATAACCCATTTGAAAT+GGG - chr7.1:21125711-21125730 MS.gene033704:CDS 30.0%
! CCATATACCTTGCTAATTTT+AGG + chr7.1:21126275-21126294 None:intergenic 30.0%
! GATGTTTATATGCCTCTTTT+AGG - chr7.1:21127556-21127575 MS.gene033704:CDS 30.0%
! GTTTGTGACAATAATTTGCA+TGG + chr7.1:21127167-21127186 None:intergenic 30.0%
! TTTGAAATGGGGTTTGAAAA+TGG - chr7.1:21125723-21125742 MS.gene033704:CDS 30.0%
! TTTTCAAACCCCATTTCAAA+TGG + chr7.1:21125723-21125742 None:intergenic 30.0%
!! AGAGTATTATGAACTTGTTG+AGG + chr7.1:21125348-21125367 None:intergenic 30.0%
!! TTGTTTCTGAAATTTACAGG+TGG - chr7.1:21127282-21127301 MS.gene033704:CDS 30.0%
AACAAGAATCACCTTCTTCA+TGG - chr7.1:21125400-21125419 MS.gene033704:intron 35.0%
CAAAAATTACCTTGGGAGAT+TGG + chr7.1:21127084-21127103 None:intergenic 35.0%
CATGAGAAAATTGTCTGGTA+AGG + chr7.1:21125604-21125623 None:intergenic 35.0%
CATTGCAATAGCTATTGCTA+TGG - chr7.1:21125467-21125486 MS.gene033704:intron 35.0%
TATCATTGAGTTTCTTCCTC+CGG + chr7.1:21126226-21126245 None:intergenic 35.0%
TCAGTTATTTGCCATGTTAC+TGG + chr7.1:21127203-21127222 None:intergenic 35.0%
TGTTGTGCCTAAAATTAGCA+AGG - chr7.1:21126265-21126284 MS.gene033704:intron 35.0%
TTGTTGTGAAAGAAAGAGTG+TGG + chr7.1:21125663-21125682 None:intergenic 35.0%
! CCAATAACCCATTTGAAATG+GGG - chr7.1:21125712-21125731 MS.gene033704:CDS 35.0%
! GCTGTTATCAGTTGTTTCAA+TGG - chr7.1:21127421-21127440 MS.gene033704:CDS 35.0%
! GTTGTTGTTGTTATCAGGTT+CGG + chr7.1:21125532-21125551 None:intergenic 35.0%
! TCAAATTCCCCTGTTTTCAT+GGG - chr7.1:21125771-21125790 MS.gene033704:intron 35.0%
! TGAGGTATTAGTTCTTTCTC+CGG - chr7.1:21127468-21127487 MS.gene033704:CDS 35.0%
! TGATGATGATGATGTTGTTG+TGG + chr7.1:21125906-21125925 None:intergenic 35.0%
! TTCAAATTCCCCTGTTTTCA+TGG - chr7.1:21125770-21125789 MS.gene033704:intron 35.0%
! TTGTTGTTGTTATCAGGTTC+GGG + chr7.1:21125531-21125550 None:intergenic 35.0%
! TTTTTATGTGACCGCGATTA+AGG + chr7.1:21126458-21126477 None:intergenic 35.0%
!! ACTTTTGCTATCTACCATGA+GGG - chr7.1:21127366-21127385 MS.gene033704:CDS 35.0%
!! ATAGAAAACGAAGCACTGAA+AGG + chr7.1:21125858-21125877 None:intergenic 35.0%
!! GATTCTTGTTGTTGTTGTTG+TGG + chr7.1:21125390-21125409 None:intergenic 35.0%
!! GTATTATGAACTTGTTGAGG+TGG + chr7.1:21125345-21125364 None:intergenic 35.0%
ACAGGTGGAAGTTAGAGTAA+GGG - chr7.1:21127297-21127316 MS.gene033704:CDS 40.0%
AGATGCAGTTGACTACTTGA+AGG - chr7.1:21126886-21126905 MS.gene033704:CDS 40.0%
AGGAAACTCAGAAGCTGAAA+GGG + chr7.1:21125802-21125821 None:intergenic 40.0%
ATGGTAGATAGCAAAAGTCC+TGG + chr7.1:21127364-21127383 None:intergenic 40.0%
GAACAAGAAGAAGAGAAAGG+TGG - chr7.1:21126010-21126029 MS.gene033704:CDS 40.0%
GCAAATTAAAGCAGTGCTGT+TGG - chr7.1:21127703-21127722 MS.gene033704:CDS 40.0%
GCTTCCTGCAAAGAATTTGA+TGG - chr7.1:21126190-21126209 MS.gene033704:CDS 40.0%
GGAGGAAGAAACTCAATGAT+AGG - chr7.1:21126225-21126244 MS.gene033704:intron 40.0%
GTCAAAGAGGATCTATATCC+CGG - chr7.1:21127037-21127056 MS.gene033704:CDS 40.0%
GTTTGGGGAATCTAGTGTTT+GGG - chr7.1:21126726-21126745 MS.gene033704:intron 40.0%
TACAGGTGGAAGTTAGAGTA+AGG - chr7.1:21127296-21127315 MS.gene033704:CDS 40.0%
TCAAATGGGACTGTTGTTAC+TGG - chr7.1:21126140-21126159 MS.gene033704:CDS 40.0%
TCAGCCATCAAATTCTTTGC+AGG + chr7.1:21126197-21126216 None:intergenic 40.0%
TGGAAGTTAGAGTAAGGGAA+GGG - chr7.1:21127302-21127321 MS.gene033704:CDS 40.0%
TGGTGCATGAGAAAATTGTC+TGG + chr7.1:21125609-21125628 None:intergenic 40.0%
TGTTCGTGGTTGTTGTGTTT+AGG + chr7.1:21125444-21125463 None:intergenic 40.0%
TGTTTGGGGAATCTAGTGTT+TGG - chr7.1:21126725-21126744 MS.gene033704:intron 40.0%
TTAAATTGCGGTCGCAGTAA+TGG - chr7.1:21126381-21126400 MS.gene033704:intron 40.0%
! CAAAGAATTTGATGGCTGAG+AGG - chr7.1:21126198-21126217 MS.gene033704:intron 40.0%
! CTATTGCAATGTCTTGTTCG+TGG + chr7.1:21125458-21125477 None:intergenic 40.0%
! GTGGTTGTTGTGTTTAGGTT+CGG + chr7.1:21125439-21125458 None:intergenic 40.0%
! TGATGATGTTGTTGTGGTTG+TGG + chr7.1:21125900-21125919 None:intergenic 40.0%
! TTGTGTTTAGGTTCGGAGTT+AGG + chr7.1:21125432-21125451 None:intergenic 40.0%
!! AGCACTGCTTTAATTTGCTC+GGG + chr7.1:21127701-21127720 None:intergenic 40.0%
!! ATGAACTTGTTGAGGTGGTA+AGG + chr7.1:21125340-21125359 None:intergenic 40.0%
!! CAGCACTGCTTTAATTTGCT+CGG + chr7.1:21127702-21127721 None:intergenic 40.0%
!! CCCCATTTCAAATGGGTTAT+TGG + chr7.1:21125715-21125734 None:intergenic 40.0%
!! GACTTTTGCTATCTACCATG+AGG - chr7.1:21127365-21127384 MS.gene033704:CDS 40.0%
!! TGAACTTGTTGAGGTGGTAA+GGG + chr7.1:21125339-21125358 None:intergenic 40.0%
!!! ACCTTGGGAGATTGGTTTTT+GGG + chr7.1:21127076-21127095 None:intergenic 40.0%
!!! TACCTTGGGAGATTGGTTTT+TGG + chr7.1:21127077-21127096 None:intergenic 40.0%
AAGGTTATCCAAAGCCCTCA+TGG + chr7.1:21127383-21127402 None:intergenic 45.0%
AATGGGACTGTTGTTACTGG+TGG - chr7.1:21126143-21126162 MS.gene033704:CDS 45.0%
AGATCAGAAGGGGAAGAAGA+AGG - chr7.1:21126166-21126185 MS.gene033704:CDS 45.0%
ATCAGAAGGGGAAGAAGAAG+GGG - chr7.1:21126168-21126187 MS.gene033704:CDS 45.0%
ATGGACAGGGCTTCAATACT+CGG - chr7.1:21126863-21126882 MS.gene033704:CDS 45.0%
ATTAGACGTGTTCAGAGCTG+AGG - chr7.1:21127450-21127469 MS.gene033704:CDS 45.0%
GAGGAAACTCAGAAGCTGAA+AGG + chr7.1:21125803-21125822 None:intergenic 45.0%
GATCAGAAGGGGAAGAAGAA+GGG - chr7.1:21126167-21126186 MS.gene033704:CDS 45.0%
GCAATAGCTATTGCTATGGC+TGG - chr7.1:21125471-21125490 MS.gene033704:intron 45.0%
GGCATCACACAAGCATTTCA+AGG + chr7.1:21126684-21126703 None:intergenic 45.0%
GGGGAACAAGAAGAAGAGAA+AGG - chr7.1:21126007-21126026 MS.gene033704:CDS 45.0%
GTGGAAGTTAGAGTAAGGGA+AGG - chr7.1:21127301-21127320 MS.gene033704:CDS 45.0%
GTTACTGGTGGAGATCAGAA+GGG - chr7.1:21126155-21126174 MS.gene033704:CDS 45.0%
GTTTGAGTGAAGCTTGCAAC+CGG + chr7.1:21127490-21127509 None:intergenic 45.0%
TCTCTTCTTCTTGTTCCCCA+CGG + chr7.1:21126006-21126025 None:intergenic 45.0%
TCTTAGCAGCAATGCAGAGA+AGG - chr7.1:21127662-21127681 MS.gene033704:CDS 45.0%
TGAAAGCTTGCACTTGCAGA+AGG + chr7.1:21126980-21126999 None:intergenic 45.0%
TGTTACTGGTGGAGATCAGA+AGG - chr7.1:21126154-21126173 MS.gene033704:CDS 45.0%
TGTTGTTGTGGTTCAGTCCA+AGG + chr7.1:21125378-21125397 None:intergenic 45.0%
TTACTGGTGGAGATCAGAAG+GGG - chr7.1:21126156-21126175 MS.gene033704:CDS 45.0%
TTAGGTGTGGACCATGAAGA+AGG + chr7.1:21125414-21125433 None:intergenic 45.0%
! AATTCCCCTGTTTTCATGGG+TGG - chr7.1:21125774-21125793 MS.gene033704:intron 45.0%
! AGATCCTCTTTGACTCGACA+GGG + chr7.1:21127031-21127050 None:intergenic 45.0%
! ATGTTTTGTACTCGCAGACC+AGG - chr7.1:21127343-21127362 MS.gene033704:CDS 45.0%
! ATTCCCCTGTTTTCATGGGT+GGG - chr7.1:21125775-21125794 MS.gene033704:intron 45.0%
! GGTTGATGTAGCCTTAATCG+CGG - chr7.1:21126444-21126463 MS.gene033704:intron 45.0%
! GTGGTTCGGGTTTGAAGAAA+GGG + chr7.1:21125506-21125525 None:intergenic 45.0%
! TAGATCCTCTTTGACTCGAC+AGG + chr7.1:21127032-21127051 None:intergenic 45.0%
! TGTGGTTCGGGTTTGAAGAA+AGG + chr7.1:21125507-21125526 None:intergenic 45.0%
! TTTAGGTTCGGAGTTAGGTG+TGG + chr7.1:21125427-21125446 None:intergenic 45.0%
!! ATGAGGGCTTTGGATAACCT+TGG - chr7.1:21127382-21127401 MS.gene033704:CDS 45.0%
!! GAAAACGAAGCACTGAAAGG+TGG + chr7.1:21125855-21125874 None:intergenic 45.0%
!! GCACTGCTTTAATTTGCTCG+GGG + chr7.1:21127700-21127719 None:intergenic 45.0%
!! GGCTTTGGATAACCTTGGAT+TGG - chr7.1:21127387-21127406 MS.gene033704:CDS 45.0%
!! TTGTTATCAGGTTCGGGTTG+TGG + chr7.1:21125525-21125544 None:intergenic 45.0%
!!! CCTTGGGAGATTGGTTTTTG+GGG + chr7.1:21127075-21127094 None:intergenic 45.0%
AGAGAAAGGTGGAGAAGAGC+TGG - chr7.1:21126021-21126040 MS.gene033704:CDS 50.0%
AGCTGGGAAGAAGAAGGAAG+TGG - chr7.1:21126038-21126057 MS.gene033704:CDS 50.0%
CCCCAAAAACCAATCTCCCA+AGG - chr7.1:21127072-21127091 MS.gene033704:CDS 50.0%
CCTGCTGAACATCCAATCCA+AGG + chr7.1:21127402-21127421 None:intergenic 50.0%
GAACTGGAATCAACTCCACC+TGG - chr7.1:21126941-21126960 MS.gene033704:CDS 50.0%
GAAGAAGAAGGAAGTGGTGG+TGG - chr7.1:21126044-21126063 MS.gene033704:CDS 50.0%
GAGAAAGGTGGAGAAGAGCT+GGG - chr7.1:21126022-21126041 MS.gene033704:CDS 50.0%
GAGAAGAGCTGGGAAGAAGA+AGG - chr7.1:21126032-21126051 MS.gene033704:CDS 50.0%
GCTATCTACCATGAGGGCTT+TGG - chr7.1:21127372-21127391 MS.gene033704:CDS 50.0%
TCTTCCCTGTCGAGTCAAAG+AGG - chr7.1:21127024-21127043 MS.gene033704:CDS 50.0%
TGGGAAGAAGAAGGAAGTGG+TGG - chr7.1:21126041-21126060 MS.gene033704:CDS 50.0%
TGTGGTTGAGCTAGCTCAAG+AGG + chr7.1:21125882-21125901 None:intergenic 50.0%
! AGCAGTGCTGTTGGATTCAG+CGG - chr7.1:21127712-21127731 MS.gene033704:CDS 50.0%
! CCTTGGATTGGATGTTCAGC+AGG - chr7.1:21127399-21127418 MS.gene033704:CDS 50.0%
! GCAGCATCGAGTTCAAGACT+CGG + chr7.1:21125825-21125844 None:intergenic 50.0%
! TTGACTCGACAGGGAAGAGT+CGG + chr7.1:21127022-21127041 None:intergenic 50.0%
! TTTGGGGCTTAGCAAGTCAC+CGG + chr7.1:21127059-21127078 None:intergenic 50.0%
!! ATCAGGTTCGGGTTGTGGTT+CGG + chr7.1:21125520-21125539 None:intergenic 50.0%
!! GAAGCACTGAAAGGTGGTAC+TGG + chr7.1:21125849-21125868 None:intergenic 50.0%
ACTTGAAGGAGCTGCTGCAG+CGG - chr7.1:21126900-21126919 MS.gene033704:CDS 55.0%
AGGGACCCACCCATGAAAAC+AGG + chr7.1:21125783-21125802 None:intergenic 55.0%
ATTCCGAGGTTGGAGACCGT+GGG - chr7.1:21125987-21126006 MS.gene033704:CDS 55.0%
GCATCGAGTTCAAGACTCGG+AGG + chr7.1:21125822-21125841 None:intergenic 55.0%
GGACCCACCCATGAAAACAG+GGG + chr7.1:21125781-21125800 None:intergenic 55.0%
GGGACCCACCCATGAAAACA+GGG + chr7.1:21125782-21125801 None:intergenic 55.0%
TTGGGGCTTAGCAAGTCACC+GGG + chr7.1:21127058-21127077 None:intergenic 55.0%
! ATTTGATGGCTGAGAGGCGC+CGG - chr7.1:21126204-21126223 MS.gene033704:intron 55.0%
! GCAGTGCTGTTGGATTCAGC+GGG - chr7.1:21127713-21127732 MS.gene033704:CDS 55.0%
! TTTGCTCGGGGAGTACATCC+TGG + chr7.1:21127688-21127707 None:intergenic 55.0%
!! ACGCTGTTTCAGAAGCGTCG+TGG - chr7.1:21125954-21125973 MS.gene033704:intron 55.0%
!! ACGCTTCTGAAACAGCGTCG+TGG + chr7.1:21125952-21125971 None:intergenic 55.0%
!! CGCTGTTTCAGAAGCGTCGT+GGG - chr7.1:21125955-21125974 MS.gene033704:intron 55.0%
!! CGCTTCTGAAACAGCGTCGT+GGG + chr7.1:21125951-21125970 None:intergenic 55.0%
!! GAAGAAGGAAGTGGTGGTGG+TGG - chr7.1:21126047-21126066 MS.gene033704:CDS 55.0%
!! GCTGTTTCAGAAGCGTCGTG+GGG - chr7.1:21125956-21125975 MS.gene033704:intron 55.0%
!! TCAGGTTCGGGTTGTGGTTC+GGG + chr7.1:21125519-21125538 None:intergenic 55.0%
!! TTGGATTCAGCGGGCTACCA+TGG - chr7.1:21127722-21127741 MS.gene033704:CDS 55.0%
ACAGGGAAGAGTCGGTGGAG+TGG + chr7.1:21127014-21127033 None:intergenic 60.0%
ACTCGACAGGGAAGAGTCGG+TGG + chr7.1:21127019-21127038 None:intergenic 60.0%
CAGGGAAGAGTCGGTGGAGT+GGG + chr7.1:21127013-21127032 None:intergenic 60.0%
GAAGGTTGCAGCAGTGAGCC+AGG + chr7.1:21126962-21126981 None:intergenic 60.0%
GATTCCGAGGTTGGAGACCG+TGG - chr7.1:21125986-21126005 MS.gene033704:CDS 60.0%
GCAGCAATGCAGAGAAGGCC+AGG - chr7.1:21127667-21127686 MS.gene033704:CDS 60.0%
GTCGGTGGAGTGGGTGTCAA+CGG + chr7.1:21127004-21127023 None:intergenic 60.0%
TCGGTGGAGTGGGTGTCAAC+GGG + chr7.1:21127003-21127022 None:intergenic 60.0%
TTCCCCACGGTCTCCAACCT+CGG + chr7.1:21125993-21126012 None:intergenic 60.0%
TTCCGAGGTTGGAGACCGTG+GGG - chr7.1:21125988-21126007 MS.gene033704:CDS 60.0%
! GGCGTTGGAGATTCCGAGGT+TGG - chr7.1:21125977-21125996 MS.gene033704:intron 60.0%
!! GAAGGAAGTGGTGGTGGTGG+TGG - chr7.1:21126050-21126069 MS.gene033704:CDS 60.0%
GGTTGCAGCAGTGAGCCAGG+TGG + chr7.1:21126959-21126978 None:intergenic 65.0%
!! GTGGGGCGTTGGAGATTCCG+AGG - chr7.1:21125973-21125992 MS.gene033704:intron 65.0%
!! TCAGAAGCGTCGTGGGGCGT+TGG - chr7.1:21125962-21125981 MS.gene033704:intron 65.0%
!! TGATGGCTGAGAGGCGCCGG+AGG - chr7.1:21126207-21126226 MS.gene033704:intron 70.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 21125318 21127754 21125318 ID=MS.gene033704
chr7.1 mRNA 21125318 21127754 21125318 ID=MS.gene033704.t1;Parent=MS.gene033704
chr7.1 exon 21126786 21127754 21126786 ID=MS.gene033704.t1.exon1;Parent=MS.gene033704.t1
chr7.1 CDS 21126786 21127754 21126786 ID=cds.MS.gene033704.t1;Parent=MS.gene033704.t1
chr7.1 exon 21125979 21126209 21125979 ID=MS.gene033704.t1.exon2;Parent=MS.gene033704.t1
chr7.1 CDS 21125979 21126209 21125979 ID=cds.MS.gene033704.t1;Parent=MS.gene033704.t1
chr7.1 exon 21125601 21125771 21125601 ID=MS.gene033704.t1.exon3;Parent=MS.gene033704.t1
chr7.1 CDS 21125601 21125771 21125601 ID=cds.MS.gene033704.t1;Parent=MS.gene033704.t1
chr7.1 exon 21125318 21125401 21125318 ID=MS.gene033704.t1.exon4;Parent=MS.gene033704.t1
chr7.1 CDS 21125318 21125401 21125318 ID=cds.MS.gene033704.t1;Parent=MS.gene033704.t1
Gene Sequence

>MS.gene033704

ATGGAGAGATACACGTTACCCTTACCACCTCAACAAGTTCATAATACTCTCACTATACCTTGGACTGAACCACAACAACAACAACAAGAATCACCTTCTTCATGGTCCACACCTAACTCCGAACCTAAACACAACAACCACGAACAAGACATTGCAATAGCTATTGCTATGGCTGGTTCTTCTATCCCTTTCTTCAAACCCGAACCACAACCCGAACCTGATAACAACAACAACAACTTCTACAACAACAACAACAACGTTGTTAATGTTAATAATGTTATTCCCTTACCAGACAATTTTCTCATGCACCAAAACACAAACACCATAGATTCAATTTCAAATCCACACTCTTTCTTTCACAACAATAACAACTACTTCTTCAACAACAATAATACCAATAACCCATTTGAAATGGGGTTTGAAAATGGATTCTTTATGGGAAACAACAACACTTCAAATTCCCCTGTTTTCATGGGTGGGTCCCTTTCAGCTTCTGAGTTTCCTCCGAGTCTTGAACTCGATGCTGCTCCAGTACCACCTTTCAGTGCTTCGTTTTCTATGCCTCTTGAGCTAGCTCAACCACAACCACAACAACATCATCATCATCATCAGCAACAACAACAACAACAACCCACGACGCTGTTTCAGAAGCGTCGTGGGGCGTTGGAGATTCCGAGGTTGGAGACCGTGGGGAACAAGAAGAAGAGAAAGGTGGAGAAGAGCTGGGAAGAAGAAGGAAGTGGTGGTGGTGGTGGTGATGATGTTGATGATTTTTCTGAGTTGAATTATGATTCTGATGAGAATGGGAATGATTTGAATAATTCAAATGGGACTGTTGTTACTGGTGGAGATCAGAAGGGGAAGAAGAAGGGGCTTCCTGCAAAGAATTTGATGGCTGAGAGGCGCCGGAGGAAGAAACTCAATGATAGGTTGTATATGCTTAGGTCTGTTGTGCCTAAAATTAGCAAGGTATATGGTAGAGATTTATTTACAAAATATTGTGAAATTGGATCCTTTTAGAAAAAATATTTGATGCATATTTATATTAACTTCCATTAATGTTTTAAATTGCGGTCGCAGTAATGGATTGTGATTCTTGATATTGCAAAAAATTGTTGTCAAATGCGGTTGATGTAGCCTTAATCGCGGTCACATAAAAATAAAAATCTTATCTTGATGTCTCATGTCTTTAGACAGAATCAATGTCTCCTATAATTAGAAATGCCATTGAATAAATAGTAAAATTAGTTTTTTTTCCTTCAAAAAACATAACATTTTTGTAGTCCTTGATTTGAATAATAGAAATCATGAAGGGAATTTTATTTATCAAACTAGTTTTTTGTATTTTGTTGGTCTAATTAGTCCTTGAAATGCTTGTGTGATGCCATCTTGTTGCTTTTGTAATTTTGTTTGGGGAATCTAGTGTTTGGGAATGTTGTTGCTTATGTTATTGGCAAGTGTTGCATTTGCTTTGTGTTAATTAACAACTTAGAAAATAGAACGTGCTAATGTTAGGTATTTTCATAAATTTCATTTATTTTATAGATGGACAGGGCTTCAATACTCGGAGATGCAGTTGACTACTTGAAGGAGCTGCTGCAGCGGATTAATAATCTTCATAATGAACTGGAATCAACTCCACCTGGCTCACTGCTGCAACCTTCTGCAAGTGCAAGCTTTCACCCGTTGACACCCACTCCACCGACTCTTCCCTGTCGAGTCAAAGAGGATCTATATCCCGGTGACTTGCTAAGCCCCAAAAACCAATCTCCCAAGGTAATTTTTGTTTGCCTTAATGCGAAGTTGTTTTAAGATACTGTCAGAATTGTAGTTTGTTGCTTGCAAACCATGCAAATTATTGTCACAAACGCCTTTATCTATACCAGTAACATGGCAAATAACTGAATAAGGGTACGTTGATGCAAATTATTTGGACGGTAATTGAGGAAATTGTTTAAACTTTCTTGTTTCTGAAATTTACAGGTGGAAGTTAGAGTAAGGGAAGGGAGAGCCGTCAACATTCATATGTTTTGTACTCGCAGACCAGGACTTTTGCTATCTACCATGAGGGCTTTGGATAACCTTGGATTGGATGTTCAGCAGGCTGTTATCAGTTGTTTCAATGGCTTTGCATTAGACGTGTTCAGAGCTGAGGTATTAGTTCTTTCTCCGGTTGCAAGCTTCACTCAAACATATCCATCATAAACCTTTCTTACATTTTACTGTATGCTTGCAATTGATGTTTATATGCCTCTTTTAGGTTGTTTTTTGTGAAGTATTTTGAGTTTAAATATTTCGAGTTCACTAATTATTGGATTTCTGGTGTTGATTTAGTCTTTTTGTTTCTTAGCAGCAATGCAGAGAAGGCCAGGATGTACTCCCCGAGCAAATTAAAGCAGTGCTGTTGGATTCAGCGGGCTACCATGGTTTGAATTGA
Protein sequence

>MS.gene033704.t1

MERYTLPLPPQQVHNTLTIPWTEPQQQQQESPSSWSTPNSEPKHNNHEQDIAIAIAMAGSSIPFFKPEPQPEPDNNNNNFYNNNNNVVNVNNVIPLPDNFLMHQNTNTIDSISNPHSFFHNNNNYFFNNNNTNNPFEMGFENGFFMGNNNTSNSPVFMGGSLSASEFPPSLELDAAPVPPFSASFSMPLELAQPQPQQHHHHHQQQQQQQPTTLFQKRRGALEIPRLETVGNKKKRKVEKSWEEEGSGGGGGDDVDDFSELNYDSDENGNDLNNSNGTVVTGGDQKGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAVDYLKELLQRINNLHNELESTPPGSLLQPSASASFHPLTPTPPTLPCRVKEDLYPGDLLSPKNQSPKVEVRVREGRAVNIHMFCTRRPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQCREGQDVLPEQIKAVLLDSAGYHGLN