AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034126

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034126.t1 MTR_4g009110 80.453 353 42 3 1 328 1 351 0.0 526
MS.gene034126.t1 MTR_4g009540 72.493 349 56 6 1 327 1 331 7.91e-161 452
MS.gene034126.t1 MTR_4g009540 71.341 328 54 6 1 306 1 310 2.35e-147 417
MS.gene034126.t1 MTR_2g104550 46.707 334 147 6 1 326 1 311 4.84e-84 256
MS.gene034126.t1 MTR_2g104490 46.707 334 141 9 1 325 1 306 2.02e-79 245
MS.gene034126.t1 MTR_2g104490 45.079 315 136 9 1 306 1 287 2.47e-68 216
MS.gene034126.t1 MTR_2g104500 39.365 315 143 11 17 327 2 272 1.85e-56 184
MS.gene034126.t1 MTR_8g009020 45.200 250 114 3 91 327 84 323 2.55e-56 186
MS.gene034126.t1 MTR_8g009010 37.117 326 120 8 24 324 4 269 1.13e-50 171
MS.gene034126.t1 MTR_2g104530 41.870 246 123 5 92 331 47 278 1.71e-50 169
MS.gene034126.t1 MTR_2g104560 46.875 192 87 4 141 327 44 225 3.53e-48 162
MS.gene034126.t1 MTR_5g014520 43.404 235 124 3 96 327 102 330 2.82e-47 162
MS.gene034126.t1 MTR_7g080780 33.898 354 182 10 1 327 1 329 1.38e-46 160
MS.gene034126.t1 MTR_0246s0020 42.412 257 120 8 96 327 122 375 1.98e-46 162
MS.gene034126.t1 MTR_0250s0040 46.667 180 87 5 151 327 88 261 3.11e-46 158
MS.gene034126.t1 MTR_7g080780 33.531 337 174 9 18 327 3 316 2.50e-44 154
MS.gene034126.t1 MTR_5g014600 37.736 265 136 6 85 326 76 334 4.20e-44 154
MS.gene034126.t1 MTR_8g069740 37.415 294 143 12 25 308 6 268 1.16e-43 151
MS.gene034126.t1 MTR_4g066460 39.224 232 123 5 100 327 108 325 9.74e-43 150
MS.gene034126.t1 MTR_4g097940 47.541 183 89 4 144 325 148 324 3.17e-42 149
MS.gene034126.t1 MTR_4g067010 42.347 196 103 3 134 327 130 317 4.17e-42 149
MS.gene034126.t1 MTR_0246s0020 42.570 249 113 9 96 317 122 367 5.45e-42 149
MS.gene034126.t1 MTR_6g047570 36.800 250 139 4 82 327 98 332 7.16e-42 148
MS.gene034126.t1 MTR_5g014560 45.506 178 92 2 151 327 131 304 3.36e-41 146
MS.gene034126.t1 MTR_0011s0260 38.866 247 141 5 87 327 114 356 3.44e-41 147
MS.gene034126.t1 MTR_5g014640 32.609 322 183 8 28 326 13 323 3.52e-40 144
MS.gene034126.t1 MTR_4g097920 45.355 183 93 4 144 325 133 309 1.27e-39 142
MS.gene034126.t1 MTR_4g097920 45.355 183 93 4 144 325 148 324 1.72e-39 142
MS.gene034126.t1 MTR_0011s0210 38.057 247 143 5 87 327 114 356 2.93e-39 142
MS.gene034126.t1 MTR_0246s0050 37.190 242 125 6 90 327 99 317 3.81e-38 138
MS.gene034126.t1 MTR_2g010450 47.191 178 88 3 147 322 173 346 5.18e-38 139
MS.gene034126.t1 MTR_4g098035 37.037 243 126 6 89 327 119 338 5.18e-38 138
MS.gene034126.t1 MTR_4g066380 39.320 206 118 2 122 327 124 322 6.49e-38 138
MS.gene034126.t1 MTR_2g104650 34.314 306 150 12 25 324 6 266 6.52e-37 134
MS.gene034126.t1 MTR_4g092700 43.258 178 92 3 151 325 158 329 4.01e-36 133
MS.gene034126.t1 MTR_2g104590 41.398 186 86 4 140 324 58 221 5.05e-36 130
MS.gene034126.t1 MTR_4g097950 35.593 236 143 4 94 322 12 245 5.65e-35 128
MS.gene034126.t1 MTR_7g451650 40.000 185 93 4 141 322 23 192 2.20e-32 119
MS.gene034126.t1 MTR_4g009510 42.604 169 76 5 161 325 52 203 3.14e-31 117
MS.gene034126.t1 MTR_7g050350 42.361 144 66 3 181 322 2 130 1.29e-28 108
MS.gene034126.t1 MTR_7g451650 42.361 144 66 3 181 322 2 130 1.29e-28 108
MS.gene034126.t1 MTR_4g009420 40.123 162 75 3 161 322 53 192 3.25e-28 108
MS.gene034126.t1 MTR_7g451650 39.053 169 85 4 141 306 23 176 2.59e-26 103
MS.gene034126.t1 MTR_4g009420 39.041 146 67 3 161 306 53 176 8.38e-22 92.0
MS.gene034126.t1 MTR_8g067280 32.768 177 107 3 124 290 435 609 1.87e-18 86.7
MS.gene034126.t1 MTR_6g047550 37.762 143 67 4 82 217 112 239 1.41e-17 81.6
MS.gene034126.t1 MTR_5g014550 39.370 127 72 2 180 305 53 175 9.63e-17 77.8
MS.gene034126.t1 MTR_5g030430 28.750 160 106 2 139 290 481 640 1.38e-15 78.2
MS.gene034126.t1 MTR_8g024790 27.957 186 116 6 111 290 255 428 2.39e-15 77.0
MS.gene034126.t1 MTR_1g072320 25.758 198 130 5 142 325 458 652 3.48e-15 76.6
MS.gene034126.t1 MTR_8g024770 27.273 176 113 5 141 306 265 435 6.23e-14 72.8
MS.gene034126.t1 MTR_7g083900 26.596 188 118 4 152 326 287 467 9.84e-13 68.9
MS.gene034126.t1 MTR_8g027495 30.769 117 66 2 150 264 457 560 1.16e-12 68.9
MS.gene034126.t1 MTR_7g083900 26.064 188 118 4 152 326 287 466 2.15e-12 68.2
MS.gene034126.t1 MTR_0194s0050 23.830 235 128 8 120 325 166 378 3.11e-12 67.4
MS.gene034126.t1 MTR_8g098275 25.000 204 120 4 150 327 243 439 5.62e-12 66.6
MS.gene034126.t1 MTR_3g498695 27.815 151 100 3 141 283 288 437 8.39e-12 66.2
MS.gene034126.t1 MTR_3g498695 27.815 151 100 3 141 283 288 437 8.69e-12 66.2
MS.gene034126.t1 MTR_8g098275 25.000 204 120 4 150 327 400 596 8.77e-12 66.2
MS.gene034126.t1 MTR_8g098275 25.000 204 120 4 150 327 307 503 1.13e-11 65.9
MS.gene034126.t1 MTR_7g117670 30.435 115 69 2 150 264 401 504 1.69e-11 65.5
MS.gene034126.t1 MTR_5g030770 28.470 281 153 11 23 283 18 270 5.01e-11 63.2
MS.gene034126.t1 MTR_5g030770 28.470 281 159 11 23 283 18 276 8.69e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034126.t1 AT4G37850 43.716 183 101 2 143 325 144 324 8.86e-41 145
MS.gene034126.t1 AT4G37850 43.716 183 101 2 143 325 144 324 8.86e-41 145
MS.gene034126.t1 AT2G22760 41.146 192 96 5 144 326 112 295 9.87e-36 131
MS.gene034126.t1 AT2G22770 38.249 217 111 5 130 325 104 318 3.15e-34 128
MS.gene034126.t1 AT2G22750 43.229 192 100 4 137 321 108 297 8.89e-34 126
MS.gene034126.t1 AT2G22750 43.229 192 100 4 137 321 132 321 1.08e-33 127
MS.gene034126.t1 AT2G22750 43.229 192 100 4 137 321 136 325 1.12e-33 127
MS.gene034126.t1 AT2G22760 43.275 171 81 4 144 306 112 274 5.74e-33 124
MS.gene034126.t1 AT2G22750 43.229 192 99 5 137 321 108 296 2.25e-32 122
MS.gene034126.t1 AT2G22750 43.229 192 99 5 137 321 136 324 2.89e-32 123
MS.gene034126.t1 AT5G46760 28.105 153 109 1 139 290 403 555 2.10e-15 77.4
MS.gene034126.t1 AT4G17880 29.936 157 97 3 139 290 404 552 3.05e-15 77.0
MS.gene034126.t1 AT1G63650 31.757 148 86 2 151 283 405 552 1.38e-14 75.1
MS.gene034126.t1 AT1G63650 31.757 148 86 2 151 283 405 552 1.38e-14 75.1
MS.gene034126.t1 AT1G63650 31.757 148 86 2 151 283 405 552 1.38e-14 75.1
MS.gene034126.t1 AT5G41315 30.719 153 86 4 151 283 441 593 1.12e-13 72.4
MS.gene034126.t1 AT5G41315 30.719 153 86 4 151 283 449 601 1.16e-13 72.4
MS.gene034126.t1 AT5G41315 30.719 153 86 4 151 283 446 598 1.20e-13 72.0
MS.gene034126.t1 AT1G32640 32.099 162 88 6 139 290 440 589 1.23e-13 72.0
MS.gene034126.t1 AT4G09820 25.926 189 115 4 138 326 350 513 1.24e-12 68.9
MS.gene034126.t1 AT3G61950 29.101 189 118 6 148 328 125 305 3.84e-12 66.6
MS.gene034126.t1 AT3G26744 29.189 185 116 5 110 283 266 446 4.11e-12 67.4
MS.gene034126.t1 AT3G61950 29.101 189 118 6 148 328 176 356 4.42e-12 66.6
MS.gene034126.t1 AT1G12860 30.714 140 84 3 152 283 268 402 4.70e-12 67.0
MS.gene034126.t1 AT1G12860 27.869 183 115 4 152 326 268 441 5.10e-12 67.0
MS.gene034126.t1 AT5G57150 27.848 158 108 3 143 294 47 204 5.46e-12 65.1
MS.gene034126.t1 AT2G46510 31.452 124 64 4 139 262 383 485 5.50e-12 67.0
MS.gene034126.t1 AT3G26744 29.189 185 116 5 110 283 266 446 5.56e-12 67.0
MS.gene034126.t1 AT3G26744 29.189 185 116 5 110 283 266 446 5.56e-12 67.0
MS.gene034126.t1 AT3G26744 29.189 185 116 5 110 283 266 446 5.56e-12 67.0
MS.gene034126.t1 AT5G57150 27.848 158 108 3 143 294 47 204 5.99e-12 64.7
MS.gene034126.t1 AT3G61950 33.793 145 81 5 148 285 125 261 6.29e-12 65.9
MS.gene034126.t1 AT5G57150 27.848 158 108 3 143 294 46 203 6.50e-12 64.7
MS.gene034126.t1 AT3G61950 33.793 145 81 5 148 285 176 312 9.67e-12 65.5
MS.gene034126.t1 AT5G57150 27.848 158 108 3 143 294 46 203 1.12e-11 64.3
MS.gene034126.t1 AT5G57150 27.848 158 108 3 143 294 47 204 1.12e-11 64.7
MS.gene034126.t1 AT1G01260 32.895 76 51 0 139 214 421 496 2.35e-11 65.1
MS.gene034126.t1 AT1G01260 32.895 76 51 0 139 214 421 496 2.35e-11 65.1
MS.gene034126.t1 AT1G01260 32.895 76 51 0 139 214 421 496 2.35e-11 65.1
MS.gene034126.t1 AT2G16910 22.801 307 165 9 38 293 190 475 2.51e-11 65.1
MS.gene034126.t1 AT5G46830 26.761 142 97 3 150 290 342 477 4.25e-11 64.3

Find 42 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACAACATCAAAACTTTC+TGG 0.157589 4.4:-89711339 None:intergenic
CTTGTGTTAATTTGAGTTCT+AGG 0.232838 4.4:+89711541 MS.gene034126:CDS
ATGACTCTTGCTTCAATATC+TGG 0.292442 4.4:-89713907 None:intergenic
ATCCATTGTGAGAAACAAAA+TGG 0.294137 4.4:+89713952 MS.gene034126:CDS
GCACTTTCAGCTACTATTCC+TGG 0.324686 4.4:+89711714 MS.gene034126:CDS
AAAGACAAAATGTATGTCTT+TGG 0.328953 4.4:-89711483 None:intergenic
GGTAGCAGTGTCTTGCCATT+TGG 0.333645 4.4:+89714027 MS.gene034126:CDS
GTAGCAGTGTCTTGCCATTT+GGG 0.353594 4.4:+89714028 MS.gene034126:CDS
CAATATCTGGAAGGATACTA+TGG 0.374591 4.4:-89713894 None:intergenic
TCACTTTAGCATCTTCTTTG+AGG 0.383325 4.4:-89714710 None:intergenic
AGAAACAAGAAGAAAATGTT+GGG 0.406463 4.4:+89713779 MS.gene034126:CDS
GAAATAGTTGATTTCCCAAA+TGG 0.414232 4.4:-89714042 None:intergenic
AATCATCATCGTCAGTGTTA+AGG 0.430939 4.4:-89711290 None:intergenic
TTTCCCAATGACTCCTCCAT+TGG 0.449389 4.4:-89711123 None:intergenic
TTCCATCACTATAATTGCTC+GGG 0.454181 4.4:+89714062 MS.gene034126:CDS
TTTCCAATGGAGGAGTCATT+GGG 0.455919 4.4:+89711120 None:intergenic
TTTATATTGCAGATGGGAGA+TGG 0.464639 4.4:+89714632 MS.gene034126:intron
CAATTTACCTTGCTCAAGCC+AGG 0.465848 4.4:-89711732 None:intergenic
CTCAAATTAACACAAGGTTC+TGG 0.471060 4.4:-89711534 None:intergenic
ACAGTGTATTGATCATATAA+TGG 0.475067 4.4:+89711650 MS.gene034126:CDS
TGTTCTTGTTCTTGTTCTTG+TGG 0.483186 4.4:-89711414 None:intergenic
GAGAAACAAGAAGAAAATGT+TGG 0.486811 4.4:+89713778 MS.gene034126:CDS
TTTCCATCACTATAATTGCT+CGG 0.489893 4.4:+89714061 MS.gene034126:CDS
ATATAATGGCAGAGAGAAAA+CGG 0.495978 4.4:+89711664 MS.gene034126:CDS
AAAACTAATCAACAAATCAA+TGG 0.499326 4.4:+89711603 MS.gene034126:CDS
ACACAAGGTTCTGGTGTAGC+TGG 0.499575 4.4:-89711525 None:intergenic
GATATTGAAGCAAGAGTCAT+GGG 0.509819 4.4:+89713910 MS.gene034126:CDS
AATCTTCAACTGTTTGTCAC+TGG 0.515596 4.4:+89714006 MS.gene034126:CDS
CAAATCAATGGAGCTAAGAA+AGG 0.524101 4.4:+89711615 MS.gene034126:CDS
TACCCGAGCAATTATAGTGA+TGG 0.541857 4.4:-89714064 None:intergenic
TTATATTGCAGATGGGAGAT+GGG 0.554862 4.4:+89714633 MS.gene034126:intron
AGATATTGAAGCAAGAGTCA+TGG 0.568127 4.4:+89713909 MS.gene034126:CDS
TACTATTCCTGGCTTGAGCA+AGG 0.585542 4.4:+89711725 MS.gene034126:CDS
AAAGCTTCAATACTTCAAGC+AGG 0.588462 4.4:+89713712 MS.gene034126:CDS
AACAAATCACAAAATTCATG+TGG 0.589942 4.4:+89713820 MS.gene034126:CDS
CTCTTGCTTCAATATCTGGA+AGG 0.599921 4.4:-89713903 None:intergenic
CTAGAACTCAAATTAACACA+AGG 0.614675 4.4:-89711540 None:intergenic
GTGTTGTAGGAATTGGATTG+CGG 0.620465 4.4:+89711237 MS.gene034126:intron
TGAAGCAAGAGTCATGGGAA+AGG 0.625639 4.4:+89713915 MS.gene034126:CDS
CAAGAACAAGAACAACAACA+TGG 0.670092 4.4:+89711423 MS.gene034126:CDS
GAAACAAGAAGAAAATGTTG+GGG 0.696078 4.4:+89713780 MS.gene034126:CDS
TTTGGAGAAGAACCTTTACG+AGG 0.704448 4.4:-89711465 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAATATATATAAAAAAA+TGG + chr4.4:89711854-89711873 MS.gene034126:intron 0.0%
!! AAGAAAAATGTATAAATAAA+AGG - chr4.4:89712943-89712962 None:intergenic 10.0%
!! AGAAAAATGTATAAATAAAA+GGG - chr4.4:89712942-89712961 None:intergenic 10.0%
!!! TATTAAATAATTAGTTTCAT+CGG + chr4.4:89711791-89711810 MS.gene034126:intron 10.0%
!! AAAAAAAATTGATACAAAAG+AGG - chr4.4:89713646-89713665 None:intergenic 15.0%
!! AAAAAAATTGATACAAAAGA+GGG - chr4.4:89713645-89713664 None:intergenic 15.0%
!! AAAATTGTGTCAATTAAATT+GGG + chr4.4:89712979-89712998 MS.gene034126:intron 15.0%
!! AATATTTCTTCAAATAACTA+AGG - chr4.4:89713460-89713479 None:intergenic 15.0%
!! ACTAATTATTTAATATGAGA+TGG - chr4.4:89711786-89711805 None:intergenic 15.0%
!! AGTTATTCAAAAAATTTAAG+AGG + chr4.4:89713174-89713193 MS.gene034126:intron 15.0%
!! ATATTTCTTCAAATAACTAA+GGG - chr4.4:89713459-89713478 None:intergenic 15.0%
!! ATTACTCAAAATTCTAATTT+AGG - chr4.4:89714431-89714450 None:intergenic 15.0%
!! ATTTAGTGTTATATTGTATT+GGG - chr4.4:89712878-89712897 None:intergenic 15.0%
!! TAATACGATGAAATAAAATA+AGG - chr4.4:89713533-89713552 None:intergenic 15.0%
!! TATTTAGTGTTATATTGTAT+TGG - chr4.4:89712879-89712898 None:intergenic 15.0%
!!! AATGCATCTTTTTAAATTTT+TGG - chr4.4:89713041-89713060 None:intergenic 15.0%
!!! ATTTTATTGATCAAAGATTA+GGG - chr4.4:89712426-89712445 None:intergenic 15.0%
!!! TATTTTATTGATCAAAGATT+AGG - chr4.4:89712427-89712446 None:intergenic 15.0%
!! AAAACTAATCAACAAATCAA+TGG + chr4.4:89711603-89711622 MS.gene034126:CDS 20.0%
!! AATTATTTAATATGAGATGG+AGG - chr4.4:89711783-89711802 None:intergenic 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG - chr4.4:89712846-89712865 None:intergenic 20.0%
!! ATATTACTTTATATTGCAGA+TGG + chr4.4:89714625-89714644 MS.gene034126:intron 20.0%
!! CAAAATTGTGTCAATTAAAT+TGG + chr4.4:89712978-89712997 MS.gene034126:intron 20.0%
!! CGGAGAAATTTATTAATTTA+CGG - chr4.4:89713296-89713315 None:intergenic 20.0%
!! CGTAAATTAATAAATTTCTC+CGG + chr4.4:89713294-89713313 MS.gene034126:intron 20.0%
!! CTCTATAAATTAATAATGTC+AGG - chr4.4:89713321-89713340 None:intergenic 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG - chr4.4:89712845-89712864 None:intergenic 20.0%
!! TATTACTTTATATTGCAGAT+GGG + chr4.4:89714626-89714645 MS.gene034126:intron 20.0%
!! TATTGTGTAGATTATTTGAA+AGG - chr4.4:89712297-89712316 None:intergenic 20.0%
!! TGTCAATTTGAAAAAGAAAA+AGG + chr4.4:89714296-89714315 MS.gene034126:intron 20.0%
!!! AAAAAGATGCATTAACTTTT+TGG + chr4.4:89713048-89713067 MS.gene034126:intron 20.0%
!!! AATGTCAATTTCAAGTTTAA+TGG + chr4.4:89713501-89713520 MS.gene034126:intron 20.0%
!!! AATTTCATTTCTTTTCTCTT+AGG + chr4.4:89713614-89713633 MS.gene034126:intron 20.0%
!!! CGGTTAATATATTTCATTTT+TGG - chr4.4:89712040-89712059 None:intergenic 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG - chr4.4:89712809-89712828 None:intergenic 20.0%
!!! TATATAATTTTGTGTGTTGT+AGG + chr4.4:89711224-89711243 MS.gene034126:intron 20.0%
!!! TCGAATTTTTAAGAATAAAC+AGG - chr4.4:89712263-89712282 None:intergenic 20.0%
!!! TGATTTTGATAATCATGAAA+AGG + chr4.4:89712659-89712678 MS.gene034126:intron 20.0%
! AAAGACAAAATGTATGTCTT+TGG - chr4.4:89711486-89711505 None:intergenic 25.0%
! AACAAATCACAAAATTCATG+TGG + chr4.4:89713820-89713839 MS.gene034126:CDS 25.0%
! AAGTATGGTTGAGAAAAATA+TGG + chr4.4:89714478-89714497 MS.gene034126:intron 25.0%
! AATGGTAACATATAAGATGT+TGG + chr4.4:89711872-89711891 MS.gene034126:intron 25.0%
! AGAAACAAGAAGAAAATGTT+GGG + chr4.4:89713779-89713798 MS.gene034126:CDS 25.0%
! ATTTAGCACTTATCAAAGTA+TGG + chr4.4:89714463-89714482 MS.gene034126:intron 25.0%
! GAAAGTGGAATATATTGTAT+TGG - chr4.4:89712920-89712939 None:intergenic 25.0%
! GGAGGTAGTATAATTTATTA+GGG - chr4.4:89711765-89711784 None:intergenic 25.0%
! TAAATTAATAATGTCAGGAC+CGG - chr4.4:89713316-89713335 None:intergenic 25.0%
! TAATCATACTGACGTATATA+CGG - chr4.4:89712727-89712746 None:intergenic 25.0%
! TGGAGGTAGTATAATTTATT+AGG - chr4.4:89711766-89711785 None:intergenic 25.0%
! TGTATAAATAAAAGGGAAAG+TGG - chr4.4:89712935-89712954 None:intergenic 25.0%
! TGTATTGTTAAAATCACATG+TGG + chr4.4:89713131-89713150 MS.gene034126:intron 25.0%
!! ACAGTGTATTGATCATATAA+TGG + chr4.4:89711650-89711669 MS.gene034126:CDS 25.0%
!! ACAGTTGAAGATTTTCAATA+TGG - chr4.4:89713999-89714018 None:intergenic 25.0%
!! ACATGGTTTGAAATCAAAAA+AGG + chr4.4:89711440-89711459 MS.gene034126:CDS 25.0%
!! TCATATTTTTCTGAGTTGTT+TGG - chr4.4:89712467-89712486 None:intergenic 25.0%
!!! AGAGTTTTAACTGTATTAGA+GGG + chr4.4:89713337-89713356 MS.gene034126:intron 25.0%
!!! ATGAATTTTGTGATTTGTTG+AGG - chr4.4:89713819-89713838 None:intergenic 25.0%
!!! CGCTATTTTACGTATAAAAA+CGG + chr4.4:89714387-89714406 MS.gene034126:intron 25.0%
!!! CTTTTTCAAATTGACATCAA+TGG - chr4.4:89714292-89714311 None:intergenic 25.0%
!!! CTTTTTTTTAGAAAATGGGT+CGG - chr4.4:89712805-89712824 None:intergenic 25.0%
!!! GACTAATTTGTCTTTTTGTT+TGG + chr4.4:89713201-89713220 MS.gene034126:intron 25.0%
!!! GCTCAATTTTTTTTGAGATA+TGG + chr4.4:89714502-89714521 MS.gene034126:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG - chr4.4:89712810-89712829 None:intergenic 25.0%
!!! TAGAGTTTTAACTGTATTAG+AGG + chr4.4:89713336-89713355 MS.gene034126:intron 25.0%
!!! TGAATTTTGTGATTTGTTGA+GGG - chr4.4:89713818-89713837 None:intergenic 25.0%
ACATGTGCAAATCAACTATA+TGG + chr4.4:89713575-89713594 MS.gene034126:intron 30.0%
ATATAATGGCAGAGAGAAAA+CGG + chr4.4:89711664-89711683 MS.gene034126:CDS 30.0%
ATCAAGATAAGATCAAGTGT+AGG + chr4.4:89712108-89712127 MS.gene034126:intron 30.0%
ATCCATTGTGAGAAACAAAA+TGG + chr4.4:89713952-89713971 MS.gene034126:CDS 30.0%
ATTTCGAACAAGTCATCATT+CGG + chr4.4:89713406-89713425 MS.gene034126:intron 30.0%
CTAGAACTCAAATTAACACA+AGG - chr4.4:89711543-89711562 None:intergenic 30.0%
GAAACAAGAAGAAAATGTTG+GGG + chr4.4:89713780-89713799 MS.gene034126:CDS 30.0%
GAGAAACAAGAAGAAAATGT+TGG + chr4.4:89713778-89713797 MS.gene034126:CDS 30.0%
TACACAATAACTAGCCAAAT+TGG + chr4.4:89712308-89712327 MS.gene034126:intron 30.0%
TATTCATGTTCTTGATGAGT+TGG + chr4.4:89714104-89714123 MS.gene034126:intron 30.0%
TCCAATTTGGCAATTTAAGA+GGG - chr4.4:89712364-89712383 None:intergenic 30.0%
TTGTGTCAATTAAATTGGGA+CGG + chr4.4:89712983-89713002 MS.gene034126:intron 30.0%
TTTCAAATATTGCCCTACAA+TGG - chr4.4:89714191-89714210 None:intergenic 30.0%
TTTCCATCACTATAATTGCT+CGG + chr4.4:89714061-89714080 MS.gene034126:CDS 30.0%
! AGTCTAGTTTACATCCAATT+TGG - chr4.4:89712325-89712344 None:intergenic 30.0%
! ATTTTGTGTGTTGTAGGAAT+TGG + chr4.4:89711230-89711249 MS.gene034126:intron 30.0%
! CTTGTGTTAATTTGAGTTCT+AGG + chr4.4:89711541-89711560 MS.gene034126:CDS 30.0%
! GAAATAGTTGATTTCCCAAA+TGG - chr4.4:89714045-89714064 None:intergenic 30.0%
! GAATTCCTCAAATTCTAAGA+TGG + chr4.4:89714147-89714166 MS.gene034126:intron 30.0%
! TAAAAAAAGGTACAATCACG+TGG - chr4.4:89713092-89713111 None:intergenic 30.0%
! TGTCTTTTGATCTTTAGTTG+TGG - chr4.4:89712168-89712187 None:intergenic 30.0%
! TTGAAATGGGTCAGTTATTT+TGG - chr4.4:89712832-89712851 None:intergenic 30.0%
!! TGAAATGGGTCAGTTATTTT+GGG - chr4.4:89712831-89712850 None:intergenic 30.0%
!! TTCCATTTTGTTTCTCACAA+TGG - chr4.4:89713957-89713976 None:intergenic 30.0%
!!! CACGATCATCATTGTTTTTA+TGG + chr4.4:89714252-89714271 MS.gene034126:intron 30.0%
!!! TTTTGATGTTGTTGGTAACA+TGG + chr4.4:89711347-89711366 MS.gene034126:CDS 30.0%
AAAGCTTCAATACTTCAAGC+AGG + chr4.4:89713712-89713731 MS.gene034126:CDS 35.0%
AATCATCATCGTCAGTGTTA+AGG - chr4.4:89711293-89711312 None:intergenic 35.0%
AATCTTCAACTGTTTGTCAC+TGG + chr4.4:89714006-89714025 MS.gene034126:CDS 35.0%
ACAAAACTCATACAAGCGAT+AGG + chr4.4:89712133-89712152 MS.gene034126:intron 35.0%
ACAATCACGTGGTTTATGAT+GGG - chr4.4:89713081-89713100 None:intergenic 35.0%
ACCCTCTTAAATTGCCAAAT+TGG + chr4.4:89712360-89712379 MS.gene034126:intron 35.0%
AGATATTGAAGCAAGAGTCA+TGG + chr4.4:89713909-89713928 MS.gene034126:CDS 35.0%
ATGACTCTTGCTTCAATATC+TGG - chr4.4:89713910-89713929 None:intergenic 35.0%
CAAAACTCATACAAGCGATA+GGG + chr4.4:89712134-89712153 MS.gene034126:intron 35.0%
CAAATCAATGGAGCTAAGAA+AGG + chr4.4:89711615-89711634 MS.gene034126:CDS 35.0%
CAAGAACAAGAACAACAACA+TGG + chr4.4:89711423-89711442 MS.gene034126:CDS 35.0%
CAATATCTGGAAGGATACTA+TGG - chr4.4:89713897-89713916 None:intergenic 35.0%
CCAACAACATCAAAACTTTC+TGG - chr4.4:89711342-89711361 None:intergenic 35.0%
CTCAAATTAACACAAGGTTC+TGG - chr4.4:89711537-89711556 None:intergenic 35.0%
CTCCAATTTGGCAATTTAAG+AGG - chr4.4:89712365-89712384 None:intergenic 35.0%
CTTGTATGTGTTTCCGAAAT+AGG - chr4.4:89712528-89712547 None:intergenic 35.0%
GATATTGAAGCAAGAGTCAT+GGG + chr4.4:89713910-89713929 MS.gene034126:CDS 35.0%
GCAATCCATCTTAGAATTTG+AGG - chr4.4:89714155-89714174 None:intergenic 35.0%
TACAATCACGTGGTTTATGA+TGG - chr4.4:89713082-89713101 None:intergenic 35.0%
TATGTGTTTCCGAAATAGGA+CGG - chr4.4:89712524-89712543 None:intergenic 35.0%
TCAAAAAAGGTTCCTCGTAA+AGG + chr4.4:89711453-89711472 MS.gene034126:CDS 35.0%
TGATAACAATGATACTCCAC+TGG + chr4.4:89712687-89712706 MS.gene034126:intron 35.0%
TGTTCTTGTTCTTGTTCTTG+TGG - chr4.4:89711417-89711436 None:intergenic 35.0%
TTATATTGCAGATGGGAGAT+GGG + chr4.4:89714633-89714652 MS.gene034126:intron 35.0%
TTCCATCACTATAATTGCTC+GGG + chr4.4:89714062-89714081 MS.gene034126:CDS 35.0%
TTTATATTGCAGATGGGAGA+TGG + chr4.4:89714632-89714651 MS.gene034126:intron 35.0%
! CTTTTGATCTTTAGTTGTGG+TGG - chr4.4:89712165-89712184 None:intergenic 35.0%
! GGGAAATTTTCATCTTGACA+TGG + chr4.4:89711140-89711159 MS.gene034126:CDS 35.0%
!! AAAGAGTATCCGTCCTATTT+CGG + chr4.4:89712512-89712531 MS.gene034126:intron 35.0%
!! AGATGGATTGCATTGATTGA+CGG + chr4.4:89714164-89714183 MS.gene034126:intron 35.0%
!! CCAGAAAGTTTTGATGTTGT+TGG + chr4.4:89711339-89711358 MS.gene034126:CDS 35.0%
!!! CAGCTTTTTGAGTACTTTCA+CGG - chr4.4:89711578-89711597 None:intergenic 35.0%
ATTGATTGACGGTCCATTGT+AGG + chr4.4:89714175-89714194 MS.gene034126:intron 40.0%
CGTATATACGGTATGACTGA+AGG - chr4.4:89712715-89712734 None:intergenic 40.0%
CTCTTGCTTCAATATCTGGA+AGG - chr4.4:89713906-89713925 None:intergenic 40.0%
GTCAATTAAATTGGGACGGA+GGG + chr4.4:89712987-89713006 MS.gene034126:intron 40.0%
GTGTTGTAGGAATTGGATTG+CGG + chr4.4:89711237-89711256 MS.gene034126:intron 40.0%
TACCCGAGCAATTATAGTGA+TGG - chr4.4:89714067-89714086 None:intergenic 40.0%
TAGGACGGATACTCTTTGTT+CGG - chr4.4:89712509-89712528 None:intergenic 40.0%
TCTGAGCAAGTTCTCCAATT+TGG - chr4.4:89712377-89712396 None:intergenic 40.0%
TGTCAATTAAATTGGGACGG+AGG + chr4.4:89712986-89713005 MS.gene034126:intron 40.0%
TTGATTGACGGTCCATTGTA+GGG + chr4.4:89714176-89714195 MS.gene034126:intron 40.0%
TTTGGAGAAGAACCTTTACG+AGG - chr4.4:89711468-89711487 None:intergenic 40.0%
! GAGGGAAGCTGTAGAATTTT+TGG + chr4.4:89713355-89713374 MS.gene034126:intron 40.0%
!! GTTTGGTCTTCGATGGATTA+TGG - chr4.4:89712450-89712469 None:intergenic 40.0%
AGGACGGATACTCTTTGTTC+GGG - chr4.4:89712508-89712527 None:intergenic 45.0%
ATGGGTCGGTTATTCCAAGA+CGG - chr4.4:89712791-89712810 None:intergenic 45.0%
CAATTTACCTTGCTCAAGCC+AGG - chr4.4:89711735-89711754 None:intergenic 45.0%
GCACTTTCAGCTACTATTCC+TGG + chr4.4:89711714-89711733 MS.gene034126:CDS 45.0%
TGAAGCAAGAGTCATGGGAA+AGG + chr4.4:89713915-89713934 MS.gene034126:CDS 45.0%
! ATCACGTGGTTTATGATGGG+CGG - chr4.4:89713078-89713097 None:intergenic 45.0%
! CTGAGTTGTTTGGTCTTCGA+TGG - chr4.4:89712457-89712476 None:intergenic 45.0%
! CTGGCTTGAGCAAGGTAAAT+TGG + chr4.4:89711733-89711752 MS.gene034126:intron 45.0%
! GTAGCAGTGTCTTGCCATTT+GGG + chr4.4:89714028-89714047 MS.gene034126:CDS 45.0%
! TACTATTCCTGGCTTGAGCA+AGG + chr4.4:89711725-89711744 MS.gene034126:CDS 45.0%
! TGTTGCTTACGGGGCATTAA+CGG - chr4.4:89712060-89712079 None:intergenic 45.0%
!! AGCACCCTATGTGTTGCTTA+CGG - chr4.4:89712071-89712090 None:intergenic 45.0%
!!! ACATTTTTATATATAAAAAA+AGG - chr4.4:89713105-89713124 None:intergenic 5.0%
!!! TTTTTTTATATATAAAAATG+TGG + chr4.4:89713104-89713123 MS.gene034126:intron 5.0%
ACGGAGGGAGTAATAGGACA+TGG - chr4.4:89712772-89712791 None:intergenic 50.0%
ACGGATACTCTTTGTTCGGG+TGG - chr4.4:89712505-89712524 None:intergenic 50.0%
ATGCCCCGTAAGCAACACAT+AGG + chr4.4:89712063-89712082 MS.gene034126:intron 50.0%
CGGATACTCTTTGTTCGGGT+GGG - chr4.4:89712504-89712523 None:intergenic 50.0%
GGTAGCAGTGTCTTGCCATT+TGG + chr4.4:89714027-89714046 MS.gene034126:CDS 50.0%
GGTATGACTGAAGGATCCAG+TGG - chr4.4:89712706-89712725 None:intergenic 50.0%
GTCGGTTATTCCAAGACGGA+GGG - chr4.4:89712787-89712806 None:intergenic 50.0%
TCCAAGACGGAGGGAGTAAT+AGG - chr4.4:89712778-89712797 None:intergenic 50.0%
TCCTATTACTCCCTCCGTCT+TGG + chr4.4:89712774-89712793 MS.gene034126:intron 50.0%
TGCCCCGTAAGCAACACATA+GGG + chr4.4:89712064-89712083 MS.gene034126:intron 50.0%
! ACACAAGGTTCTGGTGTAGC+TGG - chr4.4:89711528-89711547 None:intergenic 50.0%
!! CACCCTATGTGTTGCTTACG+GGG - chr4.4:89712069-89712088 None:intergenic 50.0%
!! GCACCCTATGTGTTGCTTAC+GGG - chr4.4:89712070-89712089 None:intergenic 50.0%
!! TCACGTGGTTTATGATGGGC+GGG - chr4.4:89713077-89713096 None:intergenic 50.0%
GGTCGGTTATTCCAAGACGG+AGG - chr4.4:89712788-89712807 None:intergenic 55.0%
!! CACGTGGTTTATGATGGGCG+GGG - chr4.4:89713076-89713095 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 89711126 89714727 89711126 ID=MS.gene034126
chr4.4 mRNA 89711126 89714727 89711126 ID=MS.gene034126.t1;Parent=MS.gene034126
chr4.4 exon 89711126 89711161 89711126 ID=MS.gene034126.t1.exon1;Parent=MS.gene034126.t1
chr4.4 CDS 89711126 89711161 89711126 ID=cds.MS.gene034126.t1;Parent=MS.gene034126.t1
chr4.4 exon 89711246 89711746 89711246 ID=MS.gene034126.t1.exon2;Parent=MS.gene034126.t1
chr4.4 CDS 89711246 89711746 89711246 ID=cds.MS.gene034126.t1;Parent=MS.gene034126.t1
chr4.4 exon 89713706 89714083 89713706 ID=MS.gene034126.t1.exon3;Parent=MS.gene034126.t1
chr4.4 CDS 89713706 89714083 89713706 ID=cds.MS.gene034126.t1;Parent=MS.gene034126.t1
chr4.4 exon 89714644 89714727 89714644 ID=MS.gene034126.t1.exon4;Parent=MS.gene034126.t1
chr4.4 CDS 89714644 89714727 89714644 ID=cds.MS.gene034126.t1;Parent=MS.gene034126.t1
Gene Sequence

>MS.gene034126

ATGGAGGAGTCATTGGGAAATTTTCATCTTGACATGGTAAAAATATTATTATTTTCATTATTTATTATGCATAGATATTTTAATTATTTGTCACACTGTATATAATTTTGTGTGTTGTAGGAATTGGATTGCGGTGATGATTATTTCATAGATGATTGTAACAACCTTAACACTGACGATGATGATTTTCTTAGAGAGATTTTGTTACAAACACCAGAAAGTTTTGATGTTGTTGGTAACATGGTTAAAAGTAACAGTTCAAACTCAATTGTGTCTCAACAACATCAACCACAAGAACAAGAACAAGAACAACAACATGGTTTGAAATCAAAAAAGGTTCCTCGTAAAGGTTCTTCTCCAAAGACATACATTTTGTCTTTTGACAATTCAACTATGATACCAGCTACACCAGAACCTTGTGTTAATTTGAGTTCTAGGAATAAACGTAGCCGTGAAAGTACTCAAAAAGCTGAAGTGAAAACTAATCAACAAATCAATGGAGCTAAGAAAGGTAGAAGCAGTTCACAGTGTATTGATCATATAATGGCAGAGAGAAAACGGAGACAAGAATTGTCTGAGAAATTCATTGCACTTTCAGCTACTATTCCTGGCTTGAGCAAGGTAAATTGGTAACTACCCTAATAAATTATACTACCTCCATCTCATATTAAATAATTAGTTTCATCGGATGCAGCATTCGTATAACATATTTTGATCATATTTTTCTATTATAATATATATAAAAAAATGGTAACATATAAGATGTTGGAATTAATCATAGTTCAAAGTCTATGAAATCATATCGCAAACTTTTTTATATAACTATCTAATTAGATTTTACTACAATCGAATTTACATAATCATATCATATGCTTTGTGTTTTTGAATGTTGTGTTATTTTTTTTAAGAAGCCAAAAATGAAATATATTAACCGTTAATGCCCCGTAAGCAACACATAGGGTGCTTGAAATGAGATTAAAGAATCAAGATAAGATCAAGTGTAGGTAACAAAACTCATACAAGCGATAGGGCTATGCCACCACAACTAAAGATCAAAAGACAAAACAATATATTTCGCTTTTAACAATGAGAATGTTAAAAGTTTTACTTTGTCAAGAAGATGATGAGTCGCAACCTGTTTATTCTTAAAAATTCGAGCATTTTTTTTCCTTTCAAATAATCTACACAATAACTAGCCAAATTGGATGTAAACTAGACTGAAGTTTCATCGAGTACCCTCTTAAATTGCCAAATTGGAGAACTTGCTCAGAGATACGCAAAGAAAAGACTATTGAATCCCTAATCTTTGATCAATAAAATACCATAATCCATCGAAGACCAAACAACTCAGAAAAATATGATCGATGTTTCTGCACCCACCCGAACAAAGAGTATCCGTCCTATTTCGGAAACACATACAAGTTTAATACTTATAATATTTAATCTTTTGAAATATAAGTTAAATTACTTTATTTTTTATATACATTTTCTATAAAAAAAAATATTAATTAATTCAACATAAACAAAAAATTATGATTTTGATAATCATGAAAAGGAAGACTGATAACAATGATACTCCACTGGATCCTTCAGTCATACCGTATATACGTCAGTATGATTAAACTCAGAAGAAGTACTGATTACCATGTCCTATTACTCCCTCCGTCTTGGAATAACCGACCCATTTTCTAAAAAAAAGTGTCCCAAAATAACTGACCCATTTCAATTTTCAATACAACTTTCATCATTCCCAATACAATATAACACTAAATAATACTACTTTTATCATTTCCAATACAATATATTCCACTTTCCCTTTTATTTATACATTTTTCTTAATATGTGTGAAAAACAAAATTGTGTCAATTAAATTGGGACGGAGGGAGTATCTTATATATGATGACTTTTTGTACCAAAAATTTAAAAAGATGCATTAACTTTTTGGTACCCCGCCCATCATAAACCACGTGATTGTACCTTTTTTTATATATAAAAATGTGGTTGTTGTATTGTTAAAATCACATGTGGACAAAACAAAATAAATAAAGAGTTATTCAAAAAATTTAAGAGGCATAGACTAATTTGTCTTTTTGTTTGGTATGTACATTATAATTACTTAAAAACTCTTTAAATTAATAATTATTAATTTATCGATAAATTAATAACTCCGTAAATTAATAAATTTCTCCGGTCCTGACATTATTAATTTATAGAGTTTTAACTGTATTAGAGGGAAGCTGTAGAATTTTTGGTGTTAAACTCAGAAATCGAACTAAAATTATTTCGAACAAGTCATCATTCGGTAGAGTTTATTAACGATTAACTTCATTCCCTTAGTTATTTGAAGAAATATTATAATACTAGTAAATTAGCGCAATGTCAATTTCAAGTTTAATGGAAATATCCTTATTTTATTTCATCGTATTAAAAAGCTATTGAAAATCGATTGACATGTGCAAATCAACTATATGGTTAAGAATATCAGTTTAATTTCATTTCTTTTCTCTTAGGCTTTTCCCTCTTTTGTATCAATTTTTTTTTGACGAAATGATCTAAAATTTTACGATTTCTTCTTTATAGACAGACAAAGCTTCAATACTTCAAGCAGGAATTGATTATGTGAAACAGCTTAAAGAACGTGTTGAGAAGCTAGAGAAACAAGAAGAAAATGTTGGGGTCACATCAATGACCCTCAACAAATCACAAAATTCATGTGGAATTAATGAAGACACAAATTCTAGCGAAACAAGTTGTGATGATGATTGTAACCATAGTATCCTTCCAGATATTGAAGCAAGAGTCATGGGAAAGGAAGTGCTTATTGAAATCCATTGTGAGAAACAAAATGGAATTGAGCTCAAATTACTTAACCATATTGAAAATCTTCAACTGTTTGTCACTGGTAGCAGTGTCTTGCCATTTGGGAAATCAACTATTTCCATCACTATAATTGCTCGGGTAAGCATCAGTAAAGTGTATATTCATGTTCTTGATGAGTTGGAAGAAAAAAAATTAAATATCGAATTCCTCAAATTCTAAGATGGATTGCATTGATTGACGGTCCATTGTAGGGCAATATTTGAAACTTAATTGATTGAAAATATTTTGTTTTAGAAGTAAAATATTCACGATCATCATTGTTTTTATGGTTGATATAGTGACTCCATTGATGTCAATTTGAAAAAGAAAAAGGAAAAATAAATAGACATCTCAAAATTCAAATTATATATTTTTTCGTTTTTTAACTATCATCAATCAGATCGCTATTTTACGTATAAAAACGGCTATGTGTCGTCGATAGTCCTAAATTAGAATTTTGAGTAATTTTTTAGTGATGATTTAGCACTTATCAAAGTATGGTTGAGAAAAATATGGAGCTCAATTTTTTTTGAGATATGGAGATATATTTGTTTTCTATAAAATGAAAAGTTTAATTACTTTCATATTTAATTATTGATTGTATAAGATGACAACAAATTGAAGTGTTGAATTGAAATATATATTACTTTATATTGCAGATGGGAGATGGGTGCAAAGTGACAGTGAATGATCTAGTGAAAAGCATTAGACAAGTGCTCTTGAAACCTCAAAGAAGATGCTAA
Protein sequence

>MS.gene034126.t1

MEESLGNFHLDMELDCGDDYFIDDCNNLNTDDDDFLREILLQTPESFDVVGNMVKSNSSNSIVSQQHQPQEQEQEQQHGLKSKKVPRKGSSPKTYILSFDNSTMIPATPEPCVNLSSRNKRSRESTQKAEVKTNQQINGAKKGRSSSQCIDHIMAERKRRQELSEKFIALSATIPGLSKTDKASILQAGIDYVKQLKERVEKLEKQEENVGVTSMTLNKSQNSCGINEDTNSSETSCDDDCNHSILPDIEARVMGKEVLIEIHCEKQNGIELKLLNHIENLQLFVTGSSVLPFGKSTISITIIARMGDGCKVTVNDLVKSIRQVLLKPQRRC