AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034236

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034236.t1 MTR_4g007130 89.731 409 14 2 1 381 1 409 0.0 746
MS.gene034236.t1 MTR_4g095700 44.444 414 166 10 23 381 7 411 1.22e-105 317
MS.gene034236.t1 MTR_5g015810 43.317 404 175 8 23 381 9 403 1.80e-102 308
MS.gene034236.t1 MTR_3g465010 43.424 403 172 14 18 380 1 387 6.75e-91 278
MS.gene034236.t1 MTR_5g015810 42.308 338 143 7 88 380 1 331 1.06e-78 245
MS.gene034236.t1 MTR_5g015810 41.237 291 132 6 122 380 2 285 3.61e-64 206
MS.gene034236.t1 MTR_4g122030 29.798 396 191 7 20 381 8 350 5.49e-44 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034236.t1 AT4G37580 44.853 408 168 8 24 381 3 403 1.24e-106 319
MS.gene034236.t1 AT2G23060 43.269 416 172 8 23 381 5 413 1.32e-106 320
MS.gene034236.t1 AT5G67430 39.750 400 177 11 23 380 8 385 2.43e-88 272
MS.gene034236.t1 AT4G37580 42.000 350 147 7 81 381 4 346 3.23e-82 255
MS.gene034236.t1 AT5G67430 38.209 335 150 9 88 380 1 320 7.31e-69 220
MS.gene034236.t1 AT2G30090 36.387 393 191 12 25 380 15 385 1.99e-64 210
MS.gene034236.t1 AT2G23060 42.353 255 132 5 135 381 32 279 9.07e-64 205

Find 83 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTAATTTGGGCACATTTA+TGG 0.122350 4.1:-88314125 MS.gene034236:CDS
TTTGTCAAACAAACTTAATT+TGG 0.149236 4.1:-88314137 MS.gene034236:CDS
TCCAAACAGGTTGCAGAATA+TGG 0.225082 4.1:-88315481 MS.gene034236:intron
ATGTGGATTTGTACATAATA+TGG 0.244584 4.1:-88313819 MS.gene034236:CDS
TTCTCTTGTTACTGACCTAT+TGG 0.257298 4.1:-88315963 MS.gene034236:CDS
GTGTTCAAATTGCAAGTTAA+AGG 0.270012 4.1:-88314010 MS.gene034236:CDS
GAAGAGTGGTGCAAACAAAA+AGG 0.293479 4.1:-88314400 MS.gene034236:CDS
TTGAAAATAGAACCTGTGTT+TGG 0.321183 4.1:+88315351 None:intergenic
CACATTGGAGGAAATTATCT+TGG 0.324781 4.1:-88313724 MS.gene034236:CDS
CGCCTACCATCGCGCTTGGC+CGG 0.331091 4.1:-88314220 MS.gene034236:CDS
AGAAGTTGTAGGAGTGATAA+GGG 0.332414 4.1:-88315449 MS.gene034236:CDS
TTGTCAAACAAACTTAATTT+GGG 0.336499 4.1:-88314136 MS.gene034236:CDS
TTTGGCACATACAGGAGATT+TGG 0.340135 4.1:-88314451 MS.gene034236:intron
TCTTCAACCTTCTTTGATGA+TGG 0.344515 4.1:+88316082 None:intergenic
TTTGGGATTTATCTAATGTA+TGG 0.345767 4.1:-88313887 MS.gene034236:CDS
TGTGGGATCACTTCTCTAAC+TGG 0.350327 4.1:+88313743 None:intergenic
GCTAGAGATGATGGTGGTTG+TGG 0.374585 4.1:-88313797 MS.gene034236:CDS
GGAGAAGAAGGAGAAGTTGT+AGG 0.384818 4.1:-88315460 MS.gene034236:CDS
TGAAAATAGAACCTGTGTTT+GGG 0.387719 4.1:+88315352 None:intergenic
AAAATCAAATCTATGTCTCT+TGG 0.388928 4.1:+88314157 None:intergenic
TCTCTTGTTACTGACCTATT+GGG 0.400220 4.1:-88315962 MS.gene034236:CDS
GCCTACCATCGCGCTTGGCC+GGG 0.411093 4.1:-88314219 MS.gene034236:CDS
GAGAAGTTGTAGGAGTGATA+AGG 0.411618 4.1:-88315450 MS.gene034236:CDS
ATTCTGCATTGGCAAACATA+TGG 0.414829 4.1:+88314185 None:intergenic
AAATCAAATCTATGTCTCTT+GGG 0.426207 4.1:+88314158 None:intergenic
TGAAGATAGCAGCTGAAACT+TGG 0.430463 4.1:-88316111 MS.gene034236:CDS
TTGGAAAGGCTTTGTGAGGT+TGG 0.433195 4.1:-88316001 MS.gene034236:CDS
CTAGAGATGATGGTGGTTGT+GGG 0.441863 4.1:-88313796 MS.gene034236:CDS
CTTTCAATTACATGTTATGC+TGG 0.442954 4.1:-88315915 MS.gene034236:intron
ACAGCAATGTTTGTGCTGAT+TGG 0.452904 4.1:+88314247 None:intergenic
AAGTGACGAATTCTGCATAT+AGG 0.481918 4.1:+88315935 None:intergenic
CTTAATATCGCATAGCTTGG+AGG 0.484798 4.1:+88314052 None:intergenic
GTTGCAGAATATGGAGAAGA+AGG 0.487530 4.1:-88315472 MS.gene034236:CDS
CAACTTATGAAGTCATTATG+TGG 0.488738 4.1:-88313836 MS.gene034236:CDS
CCTTCGCCTACCATCGCGCT+TGG 0.489542 4.1:-88314224 MS.gene034236:CDS
ACACAACATGCATGTACAAA+TGG 0.489795 4.1:+88313980 None:intergenic
TACATTAGATAAATCCCAAA+TGG 0.496017 4.1:+88313890 None:intergenic
GGCTTGTAGTGAGCATGAAC+AGG 0.497937 4.1:+88314268 None:intergenic
CATAATATGGCTAGAGATGA+TGG 0.502811 4.1:-88313806 MS.gene034236:CDS
ACATTGGAGGAAATTATCTT+GGG 0.506598 4.1:-88313723 MS.gene034236:CDS
TGCATATAGGATCACCCAAT+AGG 0.510258 4.1:+88315948 None:intergenic
GAGTCTTGAGCACATGAAGA+AGG 0.531250 4.1:-88313678 MS.gene034236:CDS
TGTAGTGAGCATGAACAGGC+TGG 0.531502 4.1:+88314272 None:intergenic
ACACTTAATATCGCATAGCT+TGG 0.542925 4.1:+88314049 None:intergenic
TTGTGACTGAAGTGAGTCAA+AGG 0.543039 4.1:-88313769 MS.gene034236:CDS
CATATGGTTGTATGTCGGCC+CGG 0.545386 4.1:+88314201 None:intergenic
GTCGGCCCGGCCAAGCGCGA+TGG 0.548720 4.1:+88314214 None:intergenic
GAGGAAATTATCTTGGGCTG+AGG 0.553326 4.1:-88313717 MS.gene034236:CDS
TCTATAATTGTACCAATCTG+AGG 0.555825 4.1:+88313631 None:intergenic
CTTTGTGAGGTTGGACAAAG+AGG 0.556865 4.1:-88315992 MS.gene034236:CDS
ATGAATGTATGCCATGGCTA+AGG 0.560501 4.1:-88313940 MS.gene034236:CDS
TAGTTGAACATTTAGAAGAG+TGG 0.560980 4.1:-88314414 MS.gene034236:CDS
TTAATATCGCATAGCTTGGA+GGG 0.562899 4.1:+88314053 None:intergenic
TAGAGATGATGGTGGTTGTG+GGG 0.570180 4.1:-88313795 MS.gene034236:CDS
AAGATAATTTCCTCCAATGT+GGG 0.570772 4.1:+88313726 None:intergenic
TTACACATGGAGGGTAAATG+TGG 0.575651 4.1:-88313863 MS.gene034236:CDS
AAAATTGGAAAGGCTTTGTG+AGG 0.581130 4.1:-88316005 MS.gene034236:CDS
TGTGACTGAAGTGAGTCAAA+GGG 0.585966 4.1:-88313768 MS.gene034236:CDS
CAAGATAATTTCCTCCAATG+TGG 0.604095 4.1:+88313725 None:intergenic
AATAGGTCAGTAACAAGAGA+AGG 0.604941 4.1:+88315965 None:intergenic
GAGAAGTGATCCCACATTGG+AGG 0.607408 4.1:-88313736 MS.gene034236:CDS
GCCCGGCCAAGCGCGATGGT+AGG 0.614800 4.1:+88314218 None:intergenic
ATGTATGGATTACACATGGA+GGG 0.621344 4.1:-88313872 MS.gene034236:CDS
CTATAATTGTACCAATCTGA+GGG 0.622462 4.1:+88313632 None:intergenic
GTGGGATCACTTCTCTAACT+GGG 0.623674 4.1:+88313744 None:intergenic
TTGACTCACTTCAGTCACAA+TGG 0.630592 4.1:+88313772 None:intergenic
AATGAAGGTAACCTTAGCCA+TGG 0.631481 4.1:+88313929 None:intergenic
GCTATGCGATATTAAGTGTG+TGG 0.632178 4.1:-88314045 MS.gene034236:CDS
TTTGTACATGCATGTTGTGT+AGG 0.632512 4.1:-88313977 MS.gene034236:CDS
TATTAGATGAATGTATGCCA+TGG 0.633115 4.1:-88313946 MS.gene034236:CDS
GCAAACATATGGTTGTATGT+CGG 0.634118 4.1:+88314196 None:intergenic
CCAAGCGCGATGGTAGGCGA+AGG 0.634299 4.1:+88314224 None:intergenic
GAAGTTGTAGGAGTGATAAG+GGG 0.639263 4.1:-88315448 MS.gene034236:CDS
ACATACAGGAGATTTGGCAT+TGG 0.644852 4.1:-88314445 MS.gene034236:intron
TCTAATGTATGGATTACACA+TGG 0.650598 4.1:-88313876 MS.gene034236:CDS
GTGTGAAAACAGTTACAAGA+GGG 0.661903 4.1:-88315423 MS.gene034236:CDS
TTAGAGAAGTGATCCCACAT+TGG 0.665411 4.1:-88313739 MS.gene034236:CDS
TCAGAGTCTCTCCCAAACAC+AGG 0.668522 4.1:-88315363 MS.gene034236:intron
TGATGAAGAGAAACACAAAG+TGG 0.670036 4.1:-88316035 MS.gene034236:CDS
TATCTTGGGCTGAGGACATG+TGG 0.676374 4.1:-88313709 MS.gene034236:CDS
TGTGTGAAAACAGTTACAAG+AGG 0.678804 4.1:-88315424 MS.gene034236:CDS
AATGTATGGATTACACATGG+AGG 0.692644 4.1:-88313873 MS.gene034236:CDS
AATATGGCTAGAGATGATGG+TGG 0.797279 4.1:-88313803 MS.gene034236:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTTATTTATTCATAAAAG+TGG + chr4.1:88314971-88314990 None:intergenic 15.0%
!! TTCTTTGTAAGAAAAATAAA+AGG - chr4.1:88314670-88314689 MS.gene034236:intron 15.0%
!!! AATAACTAAAAGATTGTTTT+TGG - chr4.1:88315238-88315257 MS.gene034236:intron 15.0%
!!! ATTTTTTTATGCTTTTAGTT+AGG + chr4.1:88314406-88314425 None:intergenic 15.0%
!! AAAAAATTCAAATATCTTCG+AGG + chr4.1:88313904-88313923 None:intergenic 20.0%
!! ATGAACCTTATAAAAACTAA+GGG - chr4.1:88314608-88314627 MS.gene034236:intron 20.0%
!! TACTAAGAAACTATAGTAAA+TGG - chr4.1:88314790-88314809 MS.gene034236:intron 20.0%
!! TATGAACCTTATAAAAACTA+AGG - chr4.1:88314607-88314626 MS.gene034236:intron 20.0%
!! TTGTCAAACAAACTTAATTT+GGG - chr4.1:88315571-88315590 MS.gene034236:intron 20.0%
!! TTTGTCAAACAAACTTAATT+TGG - chr4.1:88315570-88315589 MS.gene034236:intron 20.0%
!!! AATATCCCTTAGTTTTTATA+AGG + chr4.1:88314616-88314635 None:intergenic 20.0%
! AAAATCAAATCTATGTCTCT+TGG + chr4.1:88315553-88315572 None:intergenic 25.0%
! AAATCAAATCTATGTCTCTT+GGG + chr4.1:88315552-88315571 None:intergenic 25.0%
! AACAAAAATTACTGATGAAG+AGG + chr4.1:88316103-88316122 None:intergenic 25.0%
! ACAAAATGTGATCCAAAAAA+TGG - chr4.1:88315625-88315644 MS.gene034236:intron 25.0%
! AGAAACTATAGTAAATGGAT+AGG - chr4.1:88314795-88314814 MS.gene034236:intron 25.0%
! ATGTGGATTTGTACATAATA+TGG - chr4.1:88315888-88315907 MS.gene034236:intron 25.0%
! ATGTTATATTTGTTCCAAAC+AGG - chr4.1:88314213-88314232 MS.gene034236:CDS 25.0%
! CAAGAATTAGAAGTAAGAAT+AGG + chr4.1:88314919-88314938 None:intergenic 25.0%
! CTAAAAACATTAGGAAATGA+AGG + chr4.1:88315796-88315815 None:intergenic 25.0%
! GATAGTATACATGAAAAATG+TGG - chr4.1:88316051-88316070 MS.gene034236:CDS 25.0%
! TAAAACAATAGAGAAAGAAG+TGG - chr4.1:88314635-88314654 MS.gene034236:intron 25.0%
! TACATTAGATAAATCCCAAA+TGG + chr4.1:88315820-88315839 None:intergenic 25.0%
! TCTTAGTAAAGTAATCATAG+TGG + chr4.1:88314778-88314797 None:intergenic 25.0%
! TTAGGTTAATAGTTAGAGTT+TGG - chr4.1:88315170-88315189 MS.gene034236:intron 25.0%
! TTTGTGAACAAATTGATTGA+TGG + chr4.1:88315373-88315392 None:intergenic 25.0%
!! CATTTTGTTAGGTCTTTTTT+AGG + chr4.1:88315613-88315632 None:intergenic 25.0%
!! CTTCATTTCCTAATGTTTTT+AGG - chr4.1:88315794-88315813 MS.gene034236:intron 25.0%
!! TCTTAACCTCTATTTTATAG+AGG - chr4.1:88315000-88315019 MS.gene034236:intron 25.0%
!! TTTGGGATTTATCTAATGTA+TGG - chr4.1:88315820-88315839 MS.gene034236:intron 25.0%
!! TTTTTGGATCACATTTTGTT+AGG + chr4.1:88315624-88315643 None:intergenic 25.0%
!!! GTAAAACTTGCTTATGTTTT+AGG - chr4.1:88314319-88314338 MS.gene034236:intron 25.0%
AAGATAATTTCCTCCAATGT+GGG + chr4.1:88315984-88316003 None:intergenic 30.0%
AATGTGAGTACTCAAAATTC+AGG - chr4.1:88315395-88315414 MS.gene034236:CDS 30.0%
ACATTGGAGGAAATTATCTT+GGG - chr4.1:88315984-88316003 MS.gene034236:CDS 30.0%
AGTATTTATGACTCAAGACA+CGG + chr4.1:88314161-88314180 None:intergenic 30.0%
AGTTCTTCATCTAGACAATA+AGG - chr4.1:88314864-88314883 MS.gene034236:intron 30.0%
ATATTAGTTGTCAGCATGTT+AGG - chr4.1:88315152-88315171 MS.gene034236:intron 30.0%
CAACTTATGAAGTCATTATG+TGG - chr4.1:88315871-88315890 MS.gene034236:intron 30.0%
CAACTTTCTCATACTTCATT+TGG - chr4.1:88314836-88314855 MS.gene034236:intron 30.0%
CTAAATCATAATGTCGCATT+AGG + chr4.1:88314064-88314083 None:intergenic 30.0%
CTAGTTTCACAGTTTGATAT+TGG - chr4.1:88314182-88314201 MS.gene034236:CDS 30.0%
CTATAATTGTACCAATCTGA+GGG + chr4.1:88316078-88316097 None:intergenic 30.0%
CTTTCAATTACATGTTATGC+TGG - chr4.1:88313792-88313811 MS.gene034236:CDS 30.0%
GAATCATGGTTGAAATAGTT+CGG + chr4.1:88315077-88315096 None:intergenic 30.0%
GCATTAGGTATACATCAATA+TGG + chr4.1:88314049-88314068 None:intergenic 30.0%
GTGTTCAAATTGCAAGTTAA+AGG - chr4.1:88315697-88315716 MS.gene034236:intron 30.0%
TAGTTGAACATTTAGAAGAG+TGG - chr4.1:88315293-88315312 MS.gene034236:intron 30.0%
TATTAGATGAATGTATGCCA+TGG - chr4.1:88315761-88315780 MS.gene034236:intron 30.0%
TATTGTCTAGATGAAGAACT+TGG + chr4.1:88314864-88314883 None:intergenic 30.0%
TCCAAAGTTGTTCAAAAAGA+AGG + chr4.1:88315204-88315223 None:intergenic 30.0%
TCCACAAAAAACCTTACTTT+AGG - chr4.1:88314887-88314906 MS.gene034236:intron 30.0%
TCTATAATTGTACCAATCTG+AGG + chr4.1:88316079-88316098 None:intergenic 30.0%
TCTCTTCCTCTATAAAATAG+AGG + chr4.1:88315009-88315028 None:intergenic 30.0%
TGAAAATAGAACCTGTGTTT+GGG + chr4.1:88314358-88314377 None:intergenic 30.0%
TTGAAAATAGAACCTGTGTT+TGG + chr4.1:88314359-88314378 None:intergenic 30.0%
! ACCTTCTTTTTGAACAACTT+TGG - chr4.1:88315200-88315219 MS.gene034236:intron 30.0%
! ACTTAATTTGGGCACATTTA+TGG - chr4.1:88315582-88315601 MS.gene034236:intron 30.0%
! TCTAATGTATGGATTACACA+TGG - chr4.1:88315831-88315850 MS.gene034236:intron 30.0%
! TTCTTTTTGAACAACTTTGG+AGG - chr4.1:88315203-88315222 MS.gene034236:intron 30.0%
!!! AAGTTTTGAAATGACTTGTG+TGG + chr4.1:88314529-88314548 None:intergenic 30.0%
!!! CTAATGTTTTTAGGCCATTT+GGG - chr4.1:88315803-88315822 MS.gene034236:intron 30.0%
!!! TGTTAGGTCTTTTTTAGGTA+TGG + chr4.1:88315608-88315627 None:intergenic 30.0%
AACAATAGAGAAAGAAGTGG+TGG - chr4.1:88314638-88314657 MS.gene034236:intron 35.0%
AAGAGTGGTGCAAACAAAAA+GGG - chr4.1:88315308-88315327 MS.gene034236:intron 35.0%
AAGTGACGAATTCTGCATAT+AGG + chr4.1:88313775-88313794 None:intergenic 35.0%
AATAGGTCAGTAACAAGAGA+AGG + chr4.1:88313745-88313764 None:intergenic 35.0%
ACAATAGAGAAAGAAGTGGT+GGG - chr4.1:88314639-88314658 MS.gene034236:intron 35.0%
ACACAACATGCATGTACAAA+TGG + chr4.1:88315730-88315749 None:intergenic 35.0%
ACACTTAATATCGCATAGCT+TGG + chr4.1:88315661-88315680 None:intergenic 35.0%
AGAAGTTGTAGGAGTGATAA+GGG - chr4.1:88314258-88314277 MS.gene034236:CDS 35.0%
ATAGGATGATGCCTAAAGTA+AGG + chr4.1:88314901-88314920 None:intergenic 35.0%
CAAAGTGGCAGTAGAAAAAT+TGG - chr4.1:88313687-88313706 MS.gene034236:CDS 35.0%
CAAGATAATTTCCTCCAATG+TGG + chr4.1:88315985-88316004 None:intergenic 35.0%
CAAGTTGTTATGTGCATTCT+TGG - chr4.1:88314568-88314587 MS.gene034236:intron 35.0%
CACATTGGAGGAAATTATCT+TGG - chr4.1:88315983-88316002 MS.gene034236:CDS 35.0%
CATAATATGGCTAGAGATGA+TGG - chr4.1:88315901-88315920 MS.gene034236:intron 35.0%
CCAAATGGCCTAAAAACATT+AGG + chr4.1:88315805-88315824 None:intergenic 35.0%
GCAAAAACCATCATCAAAGA+AGG - chr4.1:88313618-88313637 MS.gene034236:CDS 35.0%
GCAAACATATGGTTGTATGT+CGG + chr4.1:88315514-88315533 None:intergenic 35.0%
GTATTTATGACTCAAGACAC+GGG + chr4.1:88314160-88314179 None:intergenic 35.0%
GTGTGAAAACAGTTACAAGA+GGG - chr4.1:88314284-88314303 MS.gene034236:CDS 35.0%
TATACATGTGTCAATGACAG+AGG + chr4.1:88314747-88314766 None:intergenic 35.0%
TATTTATGACTCAAGACACG+GGG + chr4.1:88314159-88314178 None:intergenic 35.0%
TCTCTTGTTACTGACCTATT+GGG - chr4.1:88313745-88313764 MS.gene034236:CDS 35.0%
TCTTCAACCTTCTTTGATGA+TGG + chr4.1:88313628-88313647 None:intergenic 35.0%
TCTTGGGAAAAATTCTGCAT+TGG + chr4.1:88315536-88315555 None:intergenic 35.0%
TGATGAAGAGAAACACAAAG+TGG - chr4.1:88313672-88313691 MS.gene034236:CDS 35.0%
TGGCAGTAGAAAAATTGGAA+AGG - chr4.1:88313692-88313711 MS.gene034236:CDS 35.0%
TGTGTGAAAACAGTTACAAG+AGG - chr4.1:88314283-88314302 MS.gene034236:CDS 35.0%
TTAATATCGCATAGCTTGGA+GGG + chr4.1:88315657-88315676 None:intergenic 35.0%
TTCTCTTGTTACTGACCTAT+TGG - chr4.1:88313744-88313763 MS.gene034236:CDS 35.0%
TTTGTACATGCATGTTGTGT+AGG - chr4.1:88315730-88315749 MS.gene034236:intron 35.0%
! AAAATTGGAAAGGCTTTGTG+AGG - chr4.1:88313702-88313721 MS.gene034236:CDS 35.0%
! AATGTATGGATTACACATGG+AGG - chr4.1:88315834-88315853 MS.gene034236:intron 35.0%
! ATGTATGGATTACACATGGA+GGG - chr4.1:88315835-88315854 MS.gene034236:intron 35.0%
! ATTCTGCATTGGCAAACATA+TGG + chr4.1:88315525-88315544 None:intergenic 35.0%
! GAGAATTCGAGTTTGAATCA+TGG + chr4.1:88315091-88315110 None:intergenic 35.0%
!!! AGATTGTTTTTGGCACATAC+AGG - chr4.1:88315248-88315267 MS.gene034236:intron 35.0%
!!! CCTAATGTTTTTAGGCCATT+TGG - chr4.1:88315802-88315821 MS.gene034236:intron 35.0%
!!! GCCTAAAGTAAGGTTTTTTG+TGG + chr4.1:88314891-88314910 None:intergenic 35.0%
!!! GGAGGGAAAATACCATTTTT+TGG + chr4.1:88315640-88315659 None:intergenic 35.0%
AAAAATGTGGACCCTCAGAT+TGG - chr4.1:88316064-88316083 MS.gene034236:CDS 40.0%
AATAGAGAAAGAAGTGGTGG+GGG - chr4.1:88314641-88314660 MS.gene034236:intron 40.0%
AATGAAGGTAACCTTAGCCA+TGG + chr4.1:88315781-88315800 None:intergenic 40.0%
ACATACAGGAGATTTGGCAT+TGG - chr4.1:88315262-88315281 MS.gene034236:intron 40.0%
AGAGTGGTGCAAACAAAAAG+GGG - chr4.1:88315309-88315328 MS.gene034236:intron 40.0%
AGGGGCTAAGTATGCATATA+TGG - chr4.1:88315327-88315346 MS.gene034236:intron 40.0%
ATGAATGTATGCCATGGCTA+AGG - chr4.1:88315767-88315786 MS.gene034236:intron 40.0%
CAATAGAGAAAGAAGTGGTG+GGG - chr4.1:88314640-88314659 MS.gene034236:intron 40.0%
CTTAATATCGCATAGCTTGG+AGG + chr4.1:88315658-88315677 None:intergenic 40.0%
GAAGAGTGGTGCAAACAAAA+AGG - chr4.1:88315307-88315326 MS.gene034236:intron 40.0%
GAAGTTGTAGGAGTGATAAG+GGG - chr4.1:88314259-88314278 MS.gene034236:CDS 40.0%
GAGAAGTTGTAGGAGTGATA+AGG - chr4.1:88314257-88314276 MS.gene034236:CDS 40.0%
GCTATGCGATATTAAGTGTG+TGG - chr4.1:88315662-88315681 MS.gene034236:intron 40.0%
GTTGCAGAATATGGAGAAGA+AGG - chr4.1:88314235-88314254 MS.gene034236:CDS 40.0%
TCCAAACAGGTTGCAGAATA+TGG - chr4.1:88314226-88314245 MS.gene034236:CDS 40.0%
TCCATATTCTGCAACCTGTT+TGG + chr4.1:88314230-88314249 None:intergenic 40.0%
TGAAGATAGCAGCTGAAACT+TGG - chr4.1:88313596-88313615 MS.gene034236:CDS 40.0%
TGCATATAGGATCACCCAAT+AGG + chr4.1:88313762-88313781 None:intergenic 40.0%
TGTGACTGAAGTGAGTCAAA+GGG - chr4.1:88315939-88315958 MS.gene034236:CDS 40.0%
TTACACATGGAGGGTAAATG+TGG - chr4.1:88315844-88315863 MS.gene034236:intron 40.0%
TTAGAGAAGTGATCCCACAT+TGG - chr4.1:88315968-88315987 MS.gene034236:CDS 40.0%
TTGACTCACTTCAGTCACAA+TGG + chr4.1:88315938-88315957 None:intergenic 40.0%
TTGTGACTGAAGTGAGTCAA+AGG - chr4.1:88315938-88315957 MS.gene034236:CDS 40.0%
TTTGGCACATACAGGAGATT+TGG - chr4.1:88315256-88315275 MS.gene034236:intron 40.0%
! AATATGGCTAGAGATGATGG+TGG - chr4.1:88315904-88315923 MS.gene034236:intron 40.0%
! ACAGCAATGTTTGTGCTGAT+TGG + chr4.1:88315463-88315482 None:intergenic 40.0%
AGACACGGGGAGAAAATCAT+CGG + chr4.1:88314146-88314165 None:intergenic 45.0%
CTTTGTGAGGTTGGACAAAG+AGG - chr4.1:88313715-88313734 MS.gene034236:CDS 45.0%
GAGGAAATTATCTTGGGCTG+AGG - chr4.1:88315990-88316009 MS.gene034236:CDS 45.0%
GAGTCTTGAGCACATGAAGA+AGG - chr4.1:88316029-88316048 MS.gene034236:CDS 45.0%
GGAGAAGAAGGAGAAGTTGT+AGG - chr4.1:88314247-88314266 MS.gene034236:CDS 45.0%
GTGGGATCACTTCTCTAACT+GGG + chr4.1:88315966-88315985 None:intergenic 45.0%
GTTTACGAGATATCAGCTCG+TGG + chr4.1:88313948-88313967 None:intergenic 45.0%
TGTGGGATCACTTCTCTAAC+TGG + chr4.1:88315967-88315986 None:intergenic 45.0%
! TTGGAAAGGCTTTGTGAGGT+TGG - chr4.1:88313706-88313725 MS.gene034236:CDS 45.0%
!! CTAGAGATGATGGTGGTTGT+GGG - chr4.1:88315911-88315930 MS.gene034236:intron 45.0%
!! TAGAGATGATGGTGGTTGTG+GGG - chr4.1:88315912-88315931 MS.gene034236:intron 45.0%
!! AAAAAAATAATTAATAGTTT+AGG - chr4.1:88314418-88314437 MS.gene034236:CDS 5.0%
CATATGGTTGTATGTCGGCC+CGG + chr4.1:88315509-88315528 None:intergenic 50.0%
GAGAAGTGATCCCACATTGG+AGG - chr4.1:88315971-88315990 MS.gene034236:CDS 50.0%
GGCTTGTAGTGAGCATGAAC+AGG + chr4.1:88315442-88315461 None:intergenic 50.0%
TATCTTGGGCTGAGGACATG+TGG - chr4.1:88315998-88316017 MS.gene034236:CDS 50.0%
TCAGAGTCTCTCCCAAACAC+AGG - chr4.1:88314344-88314363 MS.gene034236:intron 50.0%
TGTAGTGAGCATGAACAGGC+TGG + chr4.1:88315438-88315457 None:intergenic 50.0%
!! GCTAGAGATGATGGTGGTTG+TGG - chr4.1:88315910-88315929 MS.gene034236:intron 50.0%
CCAAGCGCGATGGTAGGCGA+AGG + chr4.1:88315486-88315505 None:intergenic 65.0%
CCTTCGCCTACCATCGCGCT+TGG - chr4.1:88315483-88315502 MS.gene034236:intron 65.0%
CGCCTACCATCGCGCTTGGC+CGG - chr4.1:88315487-88315506 MS.gene034236:intron 70.0%
GCCTACCATCGCGCTTGGCC+GGG - chr4.1:88315488-88315507 MS.gene034236:intron 70.0%
GCCCGGCCAAGCGCGATGGT+AGG + chr4.1:88315492-88315511 None:intergenic 75.0%
! GTCGGCCCGGCCAAGCGCGA+TGG + chr4.1:88315496-88315515 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 88313589 88316140 88313589 ID=MS.gene034236
chr4.1 mRNA 88313589 88316140 88313589 ID=MS.gene034236.t1;Parent=MS.gene034236
chr4.1 exon 88315916 88316140 88315916 ID=MS.gene034236.t1.exon1;Parent=MS.gene034236.t1
chr4.1 CDS 88315916 88316140 88315916 ID=cds.MS.gene034236.t1;Parent=MS.gene034236.t1
chr4.1 exon 88315364 88315494 88315364 ID=MS.gene034236.t1.exon2;Parent=MS.gene034236.t1
chr4.1 CDS 88315364 88315494 88315364 ID=cds.MS.gene034236.t1;Parent=MS.gene034236.t1
chr4.1 exon 88314394 88314459 88314394 ID=MS.gene034236.t1.exon3;Parent=MS.gene034236.t1
chr4.1 CDS 88314394 88314459 88314394 ID=cds.MS.gene034236.t1;Parent=MS.gene034236.t1
chr4.1 exon 88313589 88314312 88313589 ID=MS.gene034236.t1.exon4;Parent=MS.gene034236.t1
chr4.1 CDS 88313589 88314312 88313589 ID=cds.MS.gene034236.t1;Parent=MS.gene034236.t1
Gene Sequence

>MS.gene034236

ATGTCTTTGAAGATAGCAGCTGAAACTTGGCAAAAACCATCATCAAAGAAGGTTGAAGAAGCAAAGATAGTGATAAGAAGTTATGATGAAGAGAAACACAAAGTGGCAGTAGAAAAATTGGAAAGGCTTTGTGAGGTTGGACAAAGAGGAAAACCTTCTCTTGTTACTGACCTATTGGGTGATCCTATATGCAGAATTCGTCACTTTCAATTACATGTTATGCTGGTAACTATATCTACATGTTTCTTATTATACTCATAACTGTTTTTCATTTTCTTGATTTTTTTATTTTTTTAAAGTTGATCGAAGAGTCCTCGAAGATATTTGAATTTTTTTCTTACTGAGTCAAGCTCTTACCACGAGCTGATATCTCGTAAACACGTTATCTTGAAGTTGAATCATATAACAACGAAAAGATTATATTGACATATAAATGTGCACACTAAGAGTTGACACACCATATTGATGTATACCTAATGCGACATTATGATTTAGTGTTGAATATCGTAGTTGAACACAACAAATTGTAAATTGTAATGAATATATATTTTTTTCCGATGATTTTCTCCCCGTGTCTTGAGTCATAAATACTACTAGTTTCACAGTTTGATATTGGACTAATCAATGTTATATTTGTTCCAAACAGGTTGCAGAATATGGAGAAGAAGGAGAAGTTGTAGGAGTGATAAGGGGTTGTGTGAAAACAGTTACAAGAGGGAATTCAGCTTATGTAAAACTTGCTTATGTTTTAGGACTCAGAGTCTCTCCCAAACACAGGTTCTATTTTCAACTCTCATAAGTTTTTTGTACATTACCTAACTAAAAGCATAAAAAAATAATTAATAGTTTAGGATGTTAAATTTGCATGAGTTTTATTTTTGAGATAAAAATATCAAAAGCATGAGTCAAAGTATATATTTTTTCTCTCTATCTTAATCCACACAAGTCATTTCAAAACTTTTGCTTCTATTCTGTTTGTCAAGTTGTTATGTGCATTCTTGGCTCTAAAAAATGTCATTATGAACCTTATAAAAACTAAGGGATATTAAAACAATAGAGAAAGAAGTGGTGGGGGATTAGATTCTTTGTAAGAAAAATAAAAGGTTAATGTTTCTCGTATCATAGAAAGATGAAAAAAGTATAGTTAAGCATGCTCCTCTGTCATTGACACATGTATATGATGTATCCACTATGATTACTTTACTAAGAAACTATAGTAAATGGATAGGATGTCTTATTCTTATTTGCAACTTTCTCATACTTCATTTGGTACCAAGTTCTTCATCTAGACAATAAGGTCCACAAAAAACCTTACTTTAGGCATCATCCTATTCTTACTTCTAATTCTTGTTTGATTTTTATTTTTTTAATCTATAAATCCACTTTTATGAATAAATAAATGTTTTTTTTGTCTTAACCTCTATTTTATAGAGGAAGAGACTCTTGATAATCTTGAATTCAACTGTAAAATAAGAAAAGTCTTGACCGAACTATTTCAACCATGATTCAAACTCGAATTCTCTCGTATGATAAATAAATGTTTTCGTATATTTTTATAGACAAATATTAGTTGTCAGCATGTTAGGTTAATAGTTAGAGTTTGGACTCTAAACCTTCTTTTTGAACAACTTTGGAGGCAACAATAAACTAATAACTAAAAGATTGTTTTTGGCACATACAGGAGATTTGGCATTGGCACAAAATTAGTTGAACATTTAGAAGAGTGGTGCAAACAAAAAGGGGCTAAGTATGCATATATGGCAACTGATTGTACAAATGAGCCATCAATCAATTTGTTCACAAAAAAATGTGAGTACTCAAAATTCAGGACTCTAACAATGCTAGTCCAGCCTGTTCATGCTCACTACAAGCCAATCAGCACAAACATTGCTGTCCTTCGCCTACCATCGCGCTTGGCCGGGCCGACATACAACCATATGTTTGCCAATGCAGAATTTTTCCCAAGAGACATAGATTTGATTTTGTCAAACAAACTTAATTTGGGCACATTTATGGCCATACCTAAAAAAGACCTAACAAAATGTGATCCAAAAAATGGTATTTTCCCTCCAAGCTATGCGATATTAAGTGTGTGGAACACAAAAGAAGTGTTCAAATTGCAAGTTAAAGGAGCATCACCATTTGTACATGCATGTTGTGTAGGTACAAGATTATTAGATGAATGTATGCCATGGCTAAGGTTACCTTCATTTCCTAATGTTTTTAGGCCATTTGGGATTTATCTAATGTATGGATTACACATGGAGGGTAAATGTGGAAAACAACTTATGAAGTCATTATGTGGATTTGTACATAATATGGCTAGAGATGATGGTGGTTGTGGGGCCATTGTGACTGAAGTGAGTCAAAGGGACCCAGTTAGAGAAGTGATCCCACATTGGAGGAAATTATCTTGGGCTGAGGACATGTGGTGCATTAAGAGTCTTGAGCACATGAAGAAGGATAGTATACATGAAAAATGTGGACCCTCAGATTGGTACAATTATAGATCCTCTTCATCAGTAATTTTTGTTGATCCTCGTGATTTTTAA
Protein sequence

>MS.gene034236.t1

MSLKIAAETWQKPSSKKVEEAKIVIRSYDEEKHKVAVEKLERLCEVGQRGKPSLVTDLLGDPICRIRHFQLHVMLVAEYGEEGEVVGVIRGCVKTVTRGNSAYVKLAYVLGLRVSPKHRRFGIGTKLVEHLEEWCKQKGAKTLTMLVQPVHAHYKPISTNIAVLRLPSRLAGPTYNHMFANAEFFPRDIDLILSNKLNLGTFMAIPKKDLTKCDPKNGIFPPSYAILSVWNTKEVFKLQVKGASPFVHACCVGTRLLDECMPWLRLPSFPNVFRPFGIYLMYGLHMEGKCGKQLMKSLCGFVHNMARDDGGCGAIVTEVSQRDPVREVIPHWRKLSWAEDMWCIKSLEHMKKDSIHEKCGPSDWYNYRSSSSVIFVDPRDF