AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034456

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034456.t1 MTR_2g086450 98.128 374 5 2 1 373 17 389 0.0 744
MS.gene034456.t1 MTR_6g032990 49.254 402 114 12 22 373 35 396 1.41e-104 313
MS.gene034456.t1 MTR_5g041350 52.941 204 87 1 22 225 8 202 2.62e-67 216
MS.gene034456.t1 MTR_4g081710 53.623 207 82 4 22 225 10 205 1.94e-63 206
MS.gene034456.t1 MTR_6g444980 56.395 172 55 5 19 189 25 177 2.88e-53 181
MS.gene034456.t1 MTR_5g027440 50.802 187 81 4 22 208 18 193 6.58e-52 174
MS.gene034456.t1 MTR_6g444980 57.051 156 47 5 19 173 25 161 2.60e-47 164
MS.gene034456.t1 MTR_7g088070 43.655 197 84 3 28 221 49 221 3.13e-43 151
MS.gene034456.t1 MTR_7g088070 44.670 197 86 5 28 221 49 225 9.18e-43 150
MS.gene034456.t1 MTR_2g027800 40.079 252 123 9 11 245 19 259 5.28e-41 147
MS.gene034456.t1 MTR_2g027860 46.023 176 72 3 11 184 31 185 1.41e-40 146
MS.gene034456.t1 MTR_7g098250 45.251 179 72 4 5 178 233 390 2.13e-39 146
MS.gene034456.t1 MTR_2g027800 38.889 252 112 8 11 245 19 245 6.53e-39 140
MS.gene034456.t1 MTR_1g053835 43.850 187 73 5 11 188 236 399 6.05e-38 141
MS.gene034456.t1 MTR_1g090670 41.026 195 102 4 11 205 26 207 2.09e-37 137
MS.gene034456.t1 MTR_1g080330 43.293 164 72 2 15 178 161 303 1.30e-36 137
MS.gene034456.t1 MTR_7g093030 43.871 155 65 3 28 182 211 343 8.97e-35 132
MS.gene034456.t1 MTR_7g115530 43.590 156 66 3 29 184 218 351 4.47e-34 131
MS.gene034456.t1 MTR_1g080330 41.958 143 62 2 15 157 161 282 1.89e-28 112
MS.gene034456.t1 MTR_7g068600 43.506 154 63 5 25 175 9 141 8.96e-28 109
MS.gene034456.t1 MTR_7g115530 42.636 129 53 2 29 157 218 325 6.13e-26 106
MS.gene034456.t1 MTR_1g053800 38.372 172 84 6 17 188 165 314 3.07e-25 104
MS.gene034456.t1 MTR_5g041350 53.846 91 42 0 135 225 1 91 1.56e-24 100
MS.gene034456.t1 MTR_5g041350 52.688 93 44 0 133 225 21 113 2.96e-24 100
MS.gene034456.t1 MTR_7g115530 64.912 57 20 0 29 85 218 274 1.89e-21 93.6
MS.gene034456.t1 MTR_7g115530 64.912 57 20 0 29 85 218 274 7.40e-21 92.8
MS.gene034456.t1 MTR_7g093030 60.345 58 23 0 28 85 211 268 3.61e-20 90.1
MS.gene034456.t1 MTR_1g053830 52.381 63 30 0 25 87 14 76 7.94e-20 84.0
MS.gene034456.t1 MTR_7g115530 67.308 52 17 0 29 80 218 269 3.26e-19 87.0
MS.gene034456.t1 MTR_7g069660 62.500 56 21 0 30 85 72 127 1.77e-18 85.9
MS.gene034456.t1 MTR_7g115530 66.667 51 17 0 29 79 218 268 2.34e-18 84.7
MS.gene034456.t1 MTR_2g023580 64.151 53 19 0 30 82 56 108 7.34e-18 82.4
MS.gene034456.t1 MTR_2g084230 52.778 72 32 1 14 85 84 153 9.41e-17 81.3
MS.gene034456.t1 MTR_2g084230 58.929 56 23 0 30 85 98 153 1.04e-16 80.5
MS.gene034456.t1 MTR_8g086410 58.929 56 23 0 30 85 110 165 2.00e-16 80.5
MS.gene034456.t1 MTR_8g086410 58.929 56 23 0 30 85 112 167 2.04e-16 80.5
MS.gene034456.t1 MTR_1g112370 62.264 53 20 0 30 82 23 75 2.86e-16 79.3
MS.gene034456.t1 MTR_3g450310 50.794 63 28 1 29 91 19 78 3.51e-16 77.0
MS.gene034456.t1 MTR_0223s0040 60.377 53 21 0 30 82 19 71 4.98e-16 77.8
MS.gene034456.t1 MTR_6g071625 53.125 64 30 0 30 93 203 266 1.19e-15 77.8
MS.gene034456.t1 MTR_4g021790 52.174 69 31 2 16 83 154 221 1.30e-15 76.6
MS.gene034456.t1 MTR_2g016220 57.143 56 24 0 30 85 90 145 2.64e-15 77.0
MS.gene034456.t1 MTR_2g016220 57.143 56 24 0 30 85 90 145 2.74e-15 77.0
MS.gene034456.t1 MTR_5g029470 58.929 56 23 0 30 85 220 275 4.32e-15 76.6
MS.gene034456.t1 MTR_3g064840 58.929 56 23 0 30 85 275 330 6.15e-15 76.3
MS.gene034456.t1 MTR_8g086410 56.604 53 23 0 30 82 112 164 7.05e-15 75.9
MS.gene034456.t1 MTR_1g021520 44.318 88 42 2 30 110 127 214 1.43e-14 73.9
MS.gene034456.t1 MTR_8g066310 57.143 56 24 0 30 85 174 229 3.95e-14 73.2
MS.gene034456.t1 MTR_1g090670 34.483 145 82 4 61 205 1 132 4.47e-14 71.2
MS.gene034456.t1 MTR_2g034960 55.932 59 25 1 25 83 207 264 4.63e-14 73.9
MS.gene034456.t1 MTR_4g121020 55.932 59 25 1 25 83 107 164 6.42e-14 73.6
MS.gene034456.t1 MTR_4g121020 55.932 59 25 1 25 83 211 268 7.30e-14 73.6
MS.gene034456.t1 MTR_4g121020 55.932 59 25 1 25 83 211 268 7.44e-14 73.6
MS.gene034456.t1 MTR_4g021855 56.364 55 23 1 29 83 168 221 9.16e-14 71.6
MS.gene034456.t1 MTR_2g450070 54.237 59 26 1 25 83 209 266 9.80e-14 73.2
MS.gene034456.t1 MTR_2g450070 54.237 59 26 1 25 83 209 266 9.89e-14 73.2
MS.gene034456.t1 MTR_2g016220 58.000 50 21 0 30 79 90 139 1.22e-13 72.0
MS.gene034456.t1 MTR_7g093010 33.333 147 76 3 28 173 212 337 1.63e-13 71.2
MS.gene034456.t1 MTR_1g032570 52.542 59 27 1 25 83 127 184 2.69e-13 71.6
MS.gene034456.t1 MTR_1g032570 52.542 59 27 1 25 83 196 253 2.91e-13 71.6
MS.gene034456.t1 MTR_4g021760 55.556 54 23 1 29 82 169 221 4.46e-13 69.7
MS.gene034456.t1 MTR_4g064730 43.333 90 49 2 26 114 128 216 6.90e-13 69.3
MS.gene034456.t1 MTR_7g089010 58.182 55 22 1 29 83 91 144 9.49e-13 68.6
MS.gene034456.t1 MTR_4g113140 55.172 58 26 0 26 83 232 289 1.15e-12 69.3
MS.gene034456.t1 MTR_1g093080 56.364 55 24 0 29 83 211 265 1.19e-12 68.9
MS.gene034456.t1 MTR_4g021845 56.863 51 21 1 29 79 168 217 2.38e-12 66.2
MS.gene034456.t1 MTR_4g086835 53.448 58 27 0 26 83 271 328 4.75e-12 67.4
MS.gene034456.t1 MTR_1g013170 56.364 55 23 1 29 83 191 244 5.19e-12 67.4
MS.gene034456.t1 MTR_5g017980 44.737 76 42 0 29 104 193 268 8.06e-12 66.2
MS.gene034456.t1 MTR_4g078810 53.704 54 24 1 29 82 158 210 1.01e-11 66.2
MS.gene034456.t1 MTR_4g131600 50.847 59 28 1 25 83 197 254 1.07e-11 66.6
MS.gene034456.t1 MTR_4g131570 53.333 60 27 1 25 84 199 257 1.43e-11 66.2
MS.gene034456.t1 MTR_8g079940 46.667 75 33 2 29 97 209 282 1.45e-11 66.2
MS.gene034456.t1 MTR_2g090675 53.846 52 23 1 31 82 163 213 1.73e-11 65.5
MS.gene034456.t1 MTR_5g054300 38.947 95 49 2 12 106 186 271 2.59e-11 64.7
MS.gene034456.t1 MTR_7g026400 52.727 55 25 1 29 83 157 210 3.25e-11 65.1
MS.gene034456.t1 MTR_4g131580 53.333 60 27 1 25 84 196 254 4.46e-11 64.7
MS.gene034456.t1 MTR_3g086100 49.153 59 29 1 25 83 124 181 4.94e-11 64.3
MS.gene034456.t1 MTR_3g106220 49.153 59 29 1 25 83 194 251 5.04e-11 64.7
MS.gene034456.t1 MTR_3g086100 49.153 59 29 1 25 83 195 252 6.03e-11 64.3
MS.gene034456.t1 MTR_4g086835 53.448 58 26 1 26 83 271 327 7.21e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034456.t1 AT3G04030 53.788 396 141 12 1 373 18 394 1.86e-120 354
MS.gene034456.t1 AT5G18240 52.840 405 139 15 1 373 18 402 3.96e-119 351
MS.gene034456.t1 AT5G18240 52.840 405 139 15 1 373 18 402 3.96e-119 351
MS.gene034456.t1 AT3G04030 53.283 396 142 13 1 373 18 393 3.47e-117 345
MS.gene034456.t1 AT5G18240 51.852 405 141 15 1 373 18 400 1.08e-115 342
MS.gene034456.t1 AT3G04030 52.273 396 141 13 1 373 18 388 3.29e-113 335
MS.gene034456.t1 AT5G18240 50.864 405 141 15 1 373 18 396 8.45e-112 332
MS.gene034456.t1 AT5G18240 50.864 405 141 15 1 373 18 396 8.45e-112 332
MS.gene034456.t1 AT3G04030 48.810 336 131 11 61 373 1 318 1.44e-81 252
MS.gene034456.t1 AT3G04030 47.024 336 131 12 61 373 1 312 2.71e-74 233
MS.gene034456.t1 AT5G18240 46.377 345 128 15 61 373 1 320 1.17e-72 229
MS.gene034456.t1 AT1G69580 52.018 223 94 4 15 229 20 237 1.46e-65 211
MS.gene034456.t1 AT1G69580 51.802 222 95 4 15 229 20 236 5.62e-65 210
MS.gene034456.t1 AT4G13640 47.244 254 111 6 19 258 27 271 1.05e-61 200
MS.gene034456.t1 AT3G24120 49.528 212 86 2 3 208 15 211 3.12e-60 196
MS.gene034456.t1 AT4G13640 46.693 257 111 7 19 258 27 274 1.38e-59 195
MS.gene034456.t1 AT3G24120 48.837 215 86 3 3 208 15 214 3.74e-58 191
MS.gene034456.t1 AT1G79430 54.706 170 59 3 19 188 24 175 1.21e-52 179
MS.gene034456.t1 AT5G45580 44.949 198 86 3 14 203 8 190 1.31e-47 163
MS.gene034456.t1 AT3G12730 50.595 168 64 4 18 182 12 163 2.78e-46 158
MS.gene034456.t1 AT5G45580 45.503 189 80 3 23 203 1 174 9.45e-46 158
MS.gene034456.t1 AT2G01060 40.517 232 108 7 28 251 14 223 5.19e-42 149
MS.gene034456.t1 AT5G29000 44.805 154 67 2 28 181 187 322 8.25e-37 137
MS.gene034456.t1 AT5G29000 44.805 154 67 2 28 181 187 322 8.25e-37 137
MS.gene034456.t1 AT5G29000 44.805 154 67 2 28 181 230 365 1.51e-36 137
MS.gene034456.t1 AT3G04450 44.586 157 69 2 25 181 194 332 4.18e-36 135
MS.gene034456.t1 AT3G04450 44.586 157 69 2 25 181 194 332 4.18e-36 135
MS.gene034456.t1 AT3G04450 44.586 157 69 2 25 181 234 372 6.91e-36 136
MS.gene034456.t1 AT4G28610 38.462 208 105 5 28 235 224 408 8.34e-36 135
MS.gene034456.t1 AT5G06800 45.455 154 65 2 29 181 192 327 6.77e-35 132
MS.gene034456.t1 AT3G13040 44.654 159 67 3 29 185 241 380 6.14e-34 130
MS.gene034456.t1 AT3G13040 44.654 159 67 3 29 185 241 380 6.14e-34 130
MS.gene034456.t1 AT3G13040 44.654 159 67 3 29 185 241 380 6.14e-34 130
MS.gene034456.t1 AT5G06800 44.444 153 63 2 29 181 192 322 1.94e-33 128
MS.gene034456.t1 AT2G20400 40.854 164 69 3 31 194 233 368 6.73e-31 121
MS.gene034456.t1 AT2G20400 40.854 164 70 4 31 194 233 369 8.35e-31 121
MS.gene034456.t1 AT1G79430 48.438 128 48 3 61 188 1 110 1.54e-26 107
MS.gene034456.t1 AT2G01060 37.245 196 93 7 64 251 1 174 4.60e-24 99.8
MS.gene034456.t1 AT3G12730 44.800 125 50 4 61 182 1 109 8.47e-22 92.0
MS.gene034456.t1 AT3G04450 60.656 61 24 0 25 85 234 294 3.69e-21 93.2
MS.gene034456.t1 AT5G29000 57.746 71 28 1 28 98 230 298 4.70e-21 92.8
MS.gene034456.t1 AT5G06800 61.017 59 23 0 29 87 192 250 7.44e-20 89.0
MS.gene034456.t1 AT2G06020 48.193 83 39 2 11 89 65 147 1.81e-18 85.5
MS.gene034456.t1 AT2G38300 55.072 69 30 1 30 98 55 122 5.73e-18 84.3
MS.gene034456.t1 AT2G42660 58.065 62 25 1 30 91 51 111 1.22e-17 82.0
MS.gene034456.t1 AT1G14600 60.714 56 22 0 30 85 24 79 4.87e-17 80.5
MS.gene034456.t1 AT4G04605 60.377 53 21 0 30 82 15 67 9.35e-17 79.0
MS.gene034456.t1 AT2G40260 58.929 56 23 0 30 85 83 138 1.08e-16 81.3
MS.gene034456.t1 AT2G02060 62.264 53 20 0 30 82 30 82 1.49e-16 79.0
MS.gene034456.t1 AT2G02060 62.264 53 20 0 30 82 30 82 1.49e-16 79.0
MS.gene034456.t1 AT5G42630 55.072 69 30 1 17 85 94 161 8.70e-16 76.3
MS.gene034456.t1 AT5G16560 58.929 56 23 0 30 85 220 275 1.37e-15 78.2
MS.gene034456.t1 AT4G04580 54.717 53 24 0 30 82 15 67 1.66e-15 73.9
MS.gene034456.t1 AT5G42630 55.072 69 30 1 17 85 94 161 2.13e-15 76.3
MS.gene034456.t1 AT1G32240 58.929 56 23 0 30 85 214 269 2.24e-15 77.0
MS.gene034456.t1 AT1G32240 58.929 56 23 0 30 85 214 269 2.29e-15 77.4
MS.gene034456.t1 AT4G17695 51.562 64 31 0 30 93 165 228 6.42e-15 75.5
MS.gene034456.t1 AT5G05090 58.182 55 22 1 29 83 81 134 2.69e-13 69.7
MS.gene034456.t1 AT5G59570 55.172 58 25 1 26 83 138 194 2.95e-13 70.1
MS.gene034456.t1 AT5G59570 55.172 58 25 1 26 83 138 194 2.95e-13 70.1
MS.gene034456.t1 AT2G40970 56.897 58 24 1 26 83 102 158 3.47e-13 69.3
MS.gene034456.t1 AT5G45580 34.074 135 66 3 77 203 16 135 3.92e-13 68.6
MS.gene034456.t1 AT3G10760 50.000 70 34 1 14 83 90 158 4.70e-13 70.1
MS.gene034456.t1 AT3G16857 54.545 55 24 1 29 83 167 220 5.89e-13 70.5
MS.gene034456.t1 AT3G16857 54.545 55 24 1 29 83 237 290 6.37e-13 70.5
MS.gene034456.t1 AT3G16857 54.545 55 24 1 29 83 237 290 6.38e-13 70.5
MS.gene034456.t1 AT3G46640 56.364 55 23 1 29 83 144 197 8.23e-13 68.9
MS.gene034456.t1 AT3G46640 56.364 55 23 1 29 83 144 197 9.56e-13 68.9
MS.gene034456.t1 AT3G46640 56.364 55 23 1 29 83 144 197 9.56e-13 68.9
MS.gene034456.t1 AT2G03500 56.364 55 24 0 29 83 233 287 1.22e-12 69.3
MS.gene034456.t1 AT1G25550 56.364 55 24 0 29 83 209 263 3.11e-12 67.4
MS.gene034456.t1 AT2G01760 49.231 65 31 2 29 92 135 198 3.13e-12 67.4
MS.gene034456.t1 AT5G58080 51.471 68 32 1 16 83 181 247 4.11e-12 68.2
MS.gene034456.t1 AT2G01760 49.231 65 31 2 29 92 200 263 4.65e-12 67.4
MS.gene034456.t1 AT2G01760 49.231 65 31 2 29 92 228 291 6.68e-12 67.0
MS.gene034456.t1 AT2G20570 55.769 52 22 1 31 82 155 205 1.82e-11 65.5
MS.gene034456.t1 AT2G20570 55.769 52 22 1 31 82 171 221 2.04e-11 65.5
MS.gene034456.t1 AT1G49560 39.583 96 55 1 29 121 192 287 2.58e-11 64.7
MS.gene034456.t1 AT5G44190 50.000 54 26 1 29 82 147 199 3.14e-11 64.7
MS.gene034456.t1 AT3G25790 38.202 89 55 0 34 122 201 289 3.29e-11 64.3
MS.gene034456.t1 AT5G44190 50.000 54 26 1 29 82 147 199 4.14e-11 64.3
MS.gene034456.t1 AT3G62670 48.438 64 29 1 25 84 207 270 4.48e-11 64.3
MS.gene034456.t1 AT1G68670 53.448 58 27 0 26 83 212 269 5.11e-11 63.9

Find 59 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTCAAAATGGAGGGTT+TGG 0.270343 2.3:+12527412 MS.gene034456:CDS
ACACTTGGTAGACAGAATTT+AGG 0.292229 2.3:+12527205 MS.gene034456:CDS
ATGCAAAACCTAGATTAAAA+TGG 0.295286 2.3:+12526142 MS.gene034456:CDS
AAAACAATGAAGTACTAAAA+TGG 0.316678 2.3:+12527527 MS.gene034456:CDS
GGAAATGGTTCTAGTGATTC+AGG 0.325156 2.3:+12526102 MS.gene034456:CDS
GTTGAGAATCATTCAGATAT+TGG 0.332253 2.3:+12527649 MS.gene034456:CDS
CAATAACAACCATGTGTTCA+TGG 0.338392 2.3:+12527453 MS.gene034456:CDS
GAACAAGAAATCATTCAAAA+TGG 0.339387 2.3:+12527403 MS.gene034456:CDS
ATGACTCTTTAAATGATATA+AGG 0.363326 2.3:-12526386 None:intergenic
TAGAACCATTTCCTGTTTGA+AGG 0.369263 2.3:-12526092 None:intergenic
GTAAACAACTTGATTTGAAT+AGG 0.370306 2.3:+12527746 MS.gene034456:CDS
ATGAAACTCATGGGAATTCC+AGG 0.370323 2.3:+12526358 MS.gene034456:CDS
ACAAAGGAACACTGCAAGAT+TGG 0.379529 2.3:+12527681 MS.gene034456:CDS
AGACAGAATTTAGGAATAGT+AGG 0.380211 2.3:+12527214 MS.gene034456:CDS
TCATTTAAAGAGTCATCTTC+AGG 0.380557 2.3:+12526393 MS.gene034456:CDS
ATGTTCCTTCAAACAGGAAA+TGG 0.408546 2.3:+12526087 MS.gene034456:CDS
ATCGAGCCGTCGTTTGGTTG+AGG 0.427880 2.3:-12527340 None:intergenic
TTTATTCATGTGAGTTCCAT+TGG 0.431480 2.3:-12526689 None:intergenic
CTTTCTCAAGCACTGATTGA+AGG 0.431720 2.3:-12527174 None:intergenic
TTGATTTGAATAGGTTCAGT+TGG 0.449179 2.3:+12527755 MS.gene034456:CDS
ATAAACCTTGCATGAAGATC+AGG 0.452639 2.3:-12526168 None:intergenic
GGAAATTTGTCTATGAGCAT+TGG 0.455723 2.3:+12527625 MS.gene034456:CDS
CTGTCCATCGAGCCGTCGTT+TGG 0.455742 2.3:-12527346 None:intergenic
CTTTCCTTTCCATGAACACA+TGG 0.455778 2.3:-12527462 None:intergenic
AGGCACATGTTCCTTCAAAC+AGG 0.475299 2.3:+12526081 MS.gene034456:CDS
AATGAATTAGAAAGAAATCA+TGG 0.479388 2.3:+12527604 MS.gene034456:CDS
TACAAAGACACTTGCAGCTA+AGG 0.482388 2.3:+12527131 MS.gene034456:CDS
ATAGAAGCAGTTAATCAGTT+AGG 0.490373 2.3:+12526189 MS.gene034456:CDS
CACATGAATAAATTAACACT+TGG 0.490644 2.3:+12526700 MS.gene034456:CDS
AATTCATTATTATTTCCTAA+AGG 0.491373 2.3:-12527589 None:intergenic
ATCGTCTGACCGATCACAAA+AGG 0.496813 2.3:+12527381 MS.gene034456:CDS
GACAGAATTTAGGAATAGTA+GGG 0.543038 2.3:+12527215 MS.gene034456:CDS
CAAGAAATCATTCAAAATGG+AGG 0.547960 2.3:+12527406 MS.gene034456:CDS
AAATGATATAAGGTAAGCCC+TGG 0.549274 2.3:-12526376 None:intergenic
AAGAAATCATTCAAAATGGA+GGG 0.551325 2.3:+12527407 MS.gene034456:CDS
TCAGAGATGAAGGAGTTACA+AGG 0.555449 2.3:+12527310 MS.gene034456:CDS
CAACAAGCAACTGAATTAGC+AGG 0.559201 2.3:+12527487 MS.gene034456:CDS
GGCGCTGCAAATGCAGATTG+AGG 0.562019 2.3:+12526874 MS.gene034456:CDS
TCAACCAAACGACGGCTCGA+TGG 0.568019 2.3:+12527342 MS.gene034456:CDS
ACAACCATGTGTTCATGGAA+AGG 0.581829 2.3:+12527458 MS.gene034456:CDS
GAGAGACTTTCTGAAACCAA+TGG 0.583065 2.3:+12526673 MS.gene034456:CDS
TTGTTGTTGTTATTAGTCTG+TGG 0.584414 2.3:-12526724 None:intergenic
AAAACAGTAATGAAACTCAT+GGG 0.594613 2.3:+12526349 MS.gene034456:CDS
AGGAACATGTGCCTCTCAGA+TGG 0.596232 2.3:-12526072 None:intergenic
AAGACTCAATGAACAACTTG+AGG 0.597565 2.3:+12526904 MS.gene034456:CDS
AGATTAAGCAAGAATCTGCA+TGG 0.602970 2.3:+12526500 MS.gene034456:CDS
GAGAAAGCTCAAGAAACACT+TGG 0.604326 2.3:+12527190 MS.gene034456:CDS
CAGCTAAGGATAGAAGCTCA+AGG 0.609742 2.3:+12527145 MS.gene034456:CDS
AGTCGTGGAGATAACAATGA+AGG 0.610907 2.3:+12527712 MS.gene034456:CDS
GGACACCTGATCTTCATGCA+AGG 0.639163 2.3:+12526163 MS.gene034456:CDS
GAAGCAGTTAATCAGTTAGG+CGG 0.649355 2.3:+12526192 MS.gene034456:CDS
AGATTGGATCTAAACAGTCG+TGG 0.649789 2.3:+12527697 MS.gene034456:CDS
TGAAACTCATGGGAATTCCA+GGG 0.657200 2.3:+12526359 MS.gene034456:CDS
TGTTGAATTCAGACACTGTG+AGG 0.662262 2.3:-12527284 None:intergenic
TAGAGATTTACACATAAGCG+AGG 0.677842 2.3:+12526853 MS.gene034456:intron
AGTTAATCAGTTAGGCGGAG+CGG 0.682383 2.3:+12526197 MS.gene034456:CDS
AGTACTAAAATGGTGTGTTG+AGG 0.684708 2.3:+12527537 MS.gene034456:CDS
GAATGTCAATACCATCTGAG+AGG 0.692919 2.3:+12526061 None:intergenic
ATATTGGTGAGTTTCAACAA+AGG 0.741956 2.3:+12527665 MS.gene034456:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCATTATTATTTCCTAA+AGG - chr2.3:12527592-12527611 None:intergenic 15.0%
!! AATGAATTAGAAAGAAATCA+TGG + chr2.3:12527604-12527623 MS.gene034456:CDS 20.0%
!! ATGACTCTTTAAATGATATA+AGG - chr2.3:12526389-12526408 None:intergenic 20.0%
!!! AAAACAATGAAGTACTAAAA+TGG + chr2.3:12527527-12527546 MS.gene034456:CDS 20.0%
!!! ATGAAGAGTGTAATTAAAAA+AGG - chr2.3:12526766-12526785 None:intergenic 20.0%
!!! TGAAGAGTGTAATTAAAAAA+GGG - chr2.3:12526765-12526784 None:intergenic 20.0%
!!! TTAGTACTTCATTGTTTTTA+AGG - chr2.3:12527525-12527544 None:intergenic 20.0%
!!! TTTTTTTACCATACTTTGTA+TGG + chr2.3:12526435-12526454 MS.gene034456:intron 20.0%
! AAAAACAGTAATGAAACTCA+TGG + chr2.3:12526348-12526367 MS.gene034456:CDS 25.0%
! AAAACAGTAATGAAACTCAT+GGG + chr2.3:12526349-12526368 MS.gene034456:CDS 25.0%
! AAGAAATCATTCAAAATGGA+GGG + chr2.3:12527407-12527426 MS.gene034456:CDS 25.0%
! ATGCAAAACCTAGATTAAAA+TGG + chr2.3:12526142-12526161 MS.gene034456:CDS 25.0%
! ATGTGTAAATCTCTATCAAA+AGG - chr2.3:12526848-12526867 None:intergenic 25.0%
! CACATGAATAAATTAACACT+TGG + chr2.3:12526700-12526719 MS.gene034456:CDS 25.0%
! GAACAAGAAATCATTCAAAA+TGG + chr2.3:12527403-12527422 MS.gene034456:CDS 25.0%
! TGTGTAAATCTCTATCAAAA+GGG - chr2.3:12526847-12526866 None:intergenic 25.0%
!! ATGAGTTTCATTACTGTTTT+TGG - chr2.3:12526349-12526368 None:intergenic 25.0%
!! ATTACAAACTCATTCTCTTT+TGG + chr2.3:12526239-12526258 MS.gene034456:intron 25.0%
!! GTAAACAACTTGATTTGAAT+AGG + chr2.3:12527746-12527765 MS.gene034456:CDS 25.0%
AAACCAATATTCAAAACCTG+TGG + chr2.3:12527104-12527123 MS.gene034456:intron 30.0%
AGAAACAAAATTAACGCGTT+TGG - chr2.3:12527073-12527092 None:intergenic 30.0%
AGACAGAATTTAGGAATAGT+AGG + chr2.3:12527214-12527233 MS.gene034456:CDS 30.0%
ATAGAAGCAGTTAATCAGTT+AGG + chr2.3:12526189-12526208 MS.gene034456:CDS 30.0%
ATATTGGTGAGTTTCAACAA+AGG + chr2.3:12527665-12527684 MS.gene034456:CDS 30.0%
CAAGAAATCATTCAAAATGG+AGG + chr2.3:12527406-12527425 MS.gene034456:CDS 30.0%
GACAGAATTTAGGAATAGTA+GGG + chr2.3:12527215-12527234 MS.gene034456:CDS 30.0%
GTATAAAATCTTAGAGTGGT+AGG - chr2.3:12526812-12526831 None:intergenic 30.0%
GTCAGTATAAAATCTTAGAG+TGG - chr2.3:12526816-12526835 None:intergenic 30.0%
GTTGAGAATCATTCAGATAT+TGG + chr2.3:12527649-12527668 MS.gene034456:CDS 30.0%
TAATTGCACCATACAAAGTA+TGG - chr2.3:12526446-12526465 None:intergenic 30.0%
TCATTTAAAGAGTCATCTTC+AGG + chr2.3:12526393-12526412 MS.gene034456:CDS 30.0%
TTTATTCATGTGAGTTCCAT+TGG - chr2.3:12526692-12526711 None:intergenic 30.0%
! AGTAATTTTCTCACTCCTTT+AGG + chr2.3:12527574-12527593 MS.gene034456:CDS 30.0%
! TTCAACATTTTCAGAGATGA+AGG + chr2.3:12527300-12527319 MS.gene034456:CDS 30.0%
! TTGATTTGAATAGGTTCAGT+TGG + chr2.3:12527755-12527774 MS.gene034456:CDS 30.0%
! TTGTTGTTGTTATTAGTCTG+TGG - chr2.3:12526727-12526746 None:intergenic 30.0%
! TTTCTTGTTCCTTTTGTGAT+CGG - chr2.3:12527393-12527412 None:intergenic 30.0%
!! AAACTCATTCTCTTTTGGTT+TGG + chr2.3:12526244-12526263 MS.gene034456:intron 30.0%
AAATGATATAAGGTAAGCCC+TGG - chr2.3:12526379-12526398 None:intergenic 35.0%
AAGACTCAATGAACAACTTG+AGG + chr2.3:12526904-12526923 MS.gene034456:CDS 35.0%
ACACTTGGTAGACAGAATTT+AGG + chr2.3:12527205-12527224 MS.gene034456:CDS 35.0%
AGATTAAGCAAGAATCTGCA+TGG + chr2.3:12526500-12526519 MS.gene034456:CDS 35.0%
AGTACTAAAATGGTGTGTTG+AGG + chr2.3:12527537-12527556 MS.gene034456:CDS 35.0%
ATAAACCTTGCATGAAGATC+AGG - chr2.3:12526171-12526190 None:intergenic 35.0%
ATCATTCAAAATGGAGGGTT+TGG + chr2.3:12527412-12527431 MS.gene034456:CDS 35.0%
ATGTTCCTTCAAACAGGAAA+TGG + chr2.3:12526087-12526106 MS.gene034456:CDS 35.0%
CAATAACAACCATGTGTTCA+TGG + chr2.3:12527453-12527472 MS.gene034456:CDS 35.0%
GGAAATTTGTCTATGAGCAT+TGG + chr2.3:12527625-12527644 MS.gene034456:CDS 35.0%
TAGAACCATTTCCTGTTTGA+AGG - chr2.3:12526095-12526114 None:intergenic 35.0%
TAGAGATTTACACATAAGCG+AGG + chr2.3:12526853-12526872 MS.gene034456:intron 35.0%
TCTTGCTTAATCTGTACTTC+TGG - chr2.3:12526493-12526512 None:intergenic 35.0%
TTGCTTCTGCAATAGAAAGA+TGG - chr2.3:12526324-12526343 None:intergenic 35.0%
! TTTTATCCTCAACCAAACGA+CGG + chr2.3:12527334-12527353 MS.gene034456:CDS 35.0%
!! TCAGGTGTCCATTTTAATCT+AGG - chr2.3:12526153-12526172 None:intergenic 35.0%
ACAAAGGAACACTGCAAGAT+TGG + chr2.3:12527681-12527700 MS.gene034456:CDS 40.0%
ACAACCATGTGTTCATGGAA+AGG + chr2.3:12527458-12527477 MS.gene034456:CDS 40.0%
AGATTGGATCTAAACAGTCG+TGG + chr2.3:12527697-12527716 MS.gene034456:CDS 40.0%
AGTCGTGGAGATAACAATGA+AGG + chr2.3:12527712-12527731 MS.gene034456:CDS 40.0%
ATGAAACTCATGGGAATTCC+AGG + chr2.3:12526358-12526377 MS.gene034456:CDS 40.0%
CAACAAGCAACTGAATTAGC+AGG + chr2.3:12527487-12527506 MS.gene034456:CDS 40.0%
CAATATTCAAAACCTGTGGC+AGG + chr2.3:12527108-12527127 MS.gene034456:intron 40.0%
CTTTCCTTTCCATGAACACA+TGG - chr2.3:12527465-12527484 None:intergenic 40.0%
GAAGCAGTTAATCAGTTAGG+CGG + chr2.3:12526192-12526211 MS.gene034456:CDS 40.0%
GAGAAAGCTCAAGAAACACT+TGG + chr2.3:12527190-12527209 MS.gene034456:CDS 40.0%
TACAAAGACACTTGCAGCTA+AGG + chr2.3:12527131-12527150 MS.gene034456:CDS 40.0%
TCAGAGATGAAGGAGTTACA+AGG + chr2.3:12527310-12527329 MS.gene034456:CDS 40.0%
TCTATGTTACTCGAGATTCG+AGG - chr2.3:12526272-12526291 None:intergenic 40.0%
TGAAACTCATGGGAATTCCA+GGG + chr2.3:12526359-12526378 MS.gene034456:CDS 40.0%
TGTTGAATTCAGACACTGTG+AGG - chr2.3:12527287-12527306 None:intergenic 40.0%
! CTGTACTTCTGGTGATGAAA+AGG - chr2.3:12526482-12526501 None:intergenic 40.0%
! GAACAACTTGAGGTGAGTTT+TGG + chr2.3:12526914-12526933 MS.gene034456:intron 40.0%
!! CTTTCTCAAGCACTGATTGA+AGG - chr2.3:12527177-12527196 None:intergenic 40.0%
!! GAGAGACTTTCTGAAACCAA+TGG + chr2.3:12526673-12526692 MS.gene034456:CDS 40.0%
!! GGAAATGGTTCTAGTGATTC+AGG + chr2.3:12526102-12526121 MS.gene034456:CDS 40.0%
!!! CTGCCACAGGTTTTGAATAT+TGG - chr2.3:12527110-12527129 None:intergenic 40.0%
AGGCACATGTTCCTTCAAAC+AGG + chr2.3:12526081-12526100 MS.gene034456:CDS 45.0%
AGTTAATCAGTTAGGCGGAG+CGG + chr2.3:12526197-12526216 MS.gene034456:CDS 45.0%
ATCGTCTGACCGATCACAAA+AGG + chr2.3:12527381-12527400 MS.gene034456:CDS 45.0%
CAGCTAAGGATAGAAGCTCA+AGG + chr2.3:12527145-12527164 MS.gene034456:CDS 45.0%
TATCAGATCCTCTACAGCGA+CGG + chr2.3:12526975-12526994 MS.gene034456:intron 45.0%
AGGAACATGTGCCTCTCAGA+TGG - chr2.3:12526075-12526094 None:intergenic 50.0%
AGTGTCTTTGTACCTGCCAC+AGG - chr2.3:12527123-12527142 None:intergenic 50.0%
CGCGTTTGGATTCTCTGCAA+CGG - chr2.3:12527059-12527078 None:intergenic 50.0%
GGACACCTGATCTTCATGCA+AGG + chr2.3:12526163-12526182 MS.gene034456:CDS 50.0%
ATCGAGCCGTCGTTTGGTTG+AGG - chr2.3:12527343-12527362 None:intergenic 55.0%
GGCGCTGCAAATGCAGATTG+AGG + chr2.3:12526874-12526893 MS.gene034456:CDS 55.0%
TCAACCAAACGACGGCTCGA+TGG + chr2.3:12527342-12527361 MS.gene034456:CDS 55.0%
TCTCTGCAACGGCTGCAGAA+CGG - chr2.3:12527048-12527067 None:intergenic 55.0%
CAGCTCTACCGTCGCTGTAG+AGG - chr2.3:12526986-12527005 None:intergenic 60.0%
CTGTCCATCGAGCCGTCGTT+TGG - chr2.3:12527349-12527368 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 12526063 12527783 12526063 ID=MS.gene034456
chr2.3 mRNA 12526063 12527783 12526063 ID=MS.gene034456.t1;Parent=MS.gene034456
chr2.3 exon 12526063 12526222 12526063 ID=MS.gene034456.t1.exon1;Parent=MS.gene034456.t1
chr2.3 CDS 12526063 12526222 12526063 ID=cds.MS.gene034456.t1;Parent=MS.gene034456.t1
chr2.3 exon 12526338 12526414 12526338 ID=MS.gene034456.t1.exon2;Parent=MS.gene034456.t1
chr2.3 CDS 12526338 12526414 12526338 ID=cds.MS.gene034456.t1;Parent=MS.gene034456.t1
chr2.3 exon 12526494 12526554 12526494 ID=MS.gene034456.t1.exon3;Parent=MS.gene034456.t1
chr2.3 CDS 12526494 12526554 12526494 ID=cds.MS.gene034456.t1;Parent=MS.gene034456.t1
chr2.3 exon 12526647 12526746 12526647 ID=MS.gene034456.t1.exon4;Parent=MS.gene034456.t1
chr2.3 CDS 12526647 12526746 12526647 ID=cds.MS.gene034456.t1;Parent=MS.gene034456.t1
chr2.3 exon 12526856 12526925 12526856 ID=MS.gene034456.t1.exon5;Parent=MS.gene034456.t1
chr2.3 CDS 12526856 12526925 12526856 ID=cds.MS.gene034456.t1;Parent=MS.gene034456.t1
chr2.3 exon 12527130 12527783 12527130 ID=MS.gene034456.t1.exon6;Parent=MS.gene034456.t1
chr2.3 CDS 12527130 12527783 12527130 ID=cds.MS.gene034456.t1;Parent=MS.gene034456.t1
Gene Sequence

>MS.gene034456

ATGTCAATACCATCTGAGAGGCACATGTTCCTTCAAACAGGAAATGGTTCTAGTGATTCAGGACTTGTGCTCTCAACTGATGCAAAACCTAGATTAAAATGGACACCTGATCTTCATGCAAGGTTTATAGAAGCAGTTAATCAGTTAGGCGGAGCGGACAGTAAGTATATATACATATTACAAACTCATTCTCTTTTGGTTTGGATCCTCGAATCTCGAGTAACATAGAATTTTAATTCACTAATGAAAATTTAATTACCATCTTTCTATTGCAGAAGCAACTCCAAAAACAGTAATGAAACTCATGGGAATTCCAGGGCTTACCTTATATCATTTAAAGAGTCATCTTCAGGTAAAAATATCATTGATCTTTTTTTTTACCATACTTTGTATGGTGCAATTACTGATCATCTAATCCTTTTCATCACCAGAAGTACAGATTAAGCAAGAATCTGCATGGACAAAGCAGCAGTAATGTGACACACAAAATAAGTAAGAAACAAATTTCTTACATTTCTTCTAATATAAAGTTTAATTAATCAAAGTGCTATTTAATAACAACATACTTATATTTAATTTAGCAGACACTCATGCAGCTTCAGTGAGTGATGAGAGACTTTCTGAAACCAATGGAACTCACATGAATAAATTAACACTTGGACCACAGACTAATAACAACAACAAGTAAGAAAAACAAAACCCTTTTTTAATTACACTCTTCATGTAATCTTGTTTTATATAAAAATCCTACCACTCTAAGATTTTATACTGACTTTCATATCCCTTTTGATAGAGATTTACACATAAGCGAGGCGCTGCAAATGCAGATTGAGGTTCAGAGAAGACTCAATGAACAACTTGAGGTGAGTTTTGGCAACTTAAACTATATATGCATGTTAATTTTTTTTATCATATCAGATCCTCTACAGCGACGGTAGAGCTGTTAGATCTTAGTATCATTGATTTTGAATGTAAGATCTAACCGTTCTGCAGCCGTTGCAGAGAATCCAAACGCGTTAATTTTGTTTCTTGAATTGTAAGAAACCAATATTCAAAACCTGTGGCAGGTACAAAGACACTTGCAGCTAAGGATAGAAGCTCAAGGAAAATACCTTCAATCAGTGCTTGAGAAAGCTCAAGAAACACTTGGTAGACAGAATTTAGGAATAGTAGGGCTTGAAGCAGCAAAAGTTCAACTATCAGAGCTTGTGTCAAAAGTATCCTCACAGTGTCTGAATTCAACATTTTCAGAGATGAAGGAGTTACAAGGATTTTATCCTCAACCAAACGACGGCTCGATGGACAGTAGTTGCTTAACATCGTCTGACCGATCACAAAAGGAACAAGAAATCATTCAAAATGGAGGGTTTGGATTAAGACACTTCAACAACAATAACAACCATGTGTTCATGGAAAGGAAAGAACAACAAGCAACTGAATTAGCAGGTTCTGTTCAAAACCTTAAAAACAATGAAGTACTAAAATGGTGTGTTGAGGAAGTGAAAAAGAACAGTAATTTTCTCACTCCTTTAGGAAATAATAATGAATTAGAAAGAAATCATGGAAATTTGTCTATGAGCATTGGAGTTGAGAATCATTCAGATATTGGTGAGTTTCAACAAAGGAACACTGCAAGATTGGATCTAAACAGTCGTGGAGATAACAATGAAGGTGCAACAACTTGTAAACAACTTGATTTGAATAGGTTCAGTTGGAACTGA
Protein sequence

>MS.gene034456.t1

MSIPSERHMFLQTGNGSSDSGLVLSTDAKPRLKWTPDLHARFIEAVNQLGGADKATPKTVMKLMGIPGLTLYHLKSHLQKYRLSKNLHGQSSSNVTHKINTHAASVSDERLSETNGTHMNKLTLGPQTNNNNKDLHISEALQMQIEVQRRLNEQLEVQRHLQLRIEAQGKYLQSVLEKAQETLGRQNLGIVGLEAAKVQLSELVSKVSSQCLNSTFSEMKELQGFYPQPNDGSMDSSCLTSSDRSQKEQEIIQNGGFGLRHFNNNNNHVFMERKEQQATELAGSVQNLKNNEVLKWCVEEVKKNSNFLTPLGNNNELERNHGNLSMSIGVENHSDIGEFQQRNTARLDLNSRGDNNEGATTCKQLDLNRFSWN