AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034537


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034537.t1 MTR_1g054285 97.059 204 3 3 1 201 1 204 3.78e-141 395
MS.gene034537.t1 MTR_7g093430 60.377 212 71 8 1 201 1 210 8.57e-69 212
MS.gene034537.t1 MTR_2g061030 42.366 262 78 10 1 201 1 250 1.08e-33 123
MS.gene034537.t1 MTR_4g100550 53.659 123 46 2 81 201 57 170 4.45e-33 120
MS.gene034537.t1 MTR_5g013010 53.488 129 39 4 81 201 71 186 1.63e-32 116
MS.gene034537.t1 MTR_5g013010 53.488 129 39 4 81 201 71 186 1.03e-31 117
MS.gene034537.t1 MTR_4g097600 80.000 65 11 1 139 201 113 177 2.94e-28 107
MS.gene034537.t1 MTR_8g006705 54.054 111 45 1 97 201 86 196 2.18e-25 100
MS.gene034537.t1 MTR_5g014890 75.410 61 14 1 142 201 113 173 2.02e-23 94.4
MS.gene034537.t1 MTR_1g017090 44.444 135 55 5 79 201 54 180 5.24e-21 88.2
MS.gene034537.t1 MTR_3g103590 68.333 60 19 0 142 201 136 195 8.54e-21 88.2
MS.gene034537.t1 MTR_4g084750 73.684 57 14 1 146 201 59 115 1.17e-19 82.8
MS.gene034537.t1 MTR_4g126900 69.388 49 15 0 153 201 90 138 1.02e-16 75.5
MS.gene034537.t1 MTR_2g038625 57.353 68 28 1 134 201 66 132 3.95e-16 73.9
MS.gene034537.t1 MTR_2g038580 57.353 68 28 1 134 201 66 132 3.95e-16 73.9
MS.gene034537.t1 MTR_4g107650 50.602 83 37 2 119 201 67 145 3.19e-14 70.1
MS.gene034537.t1 MTR_7g010020 42.391 92 52 1 110 201 40 130 6.48e-14 68.9
MS.gene034537.t1 MTR_5g038280 48.611 72 34 1 130 201 69 137 2.83e-12 64.7
MS.gene034537.t1 MTR_8g089895 43.617 94 49 2 108 201 15 104 3.76e-12 64.3
MS.gene034537.t1 MTR_1g061660 50.909 55 27 0 147 201 55 109 6.07e-12 62.8
MS.gene034537.t1 MTR_6g007647 58.824 51 21 0 151 201 33 83 6.26e-12 63.2
MS.gene034537.t1 MTR_6g007647 58.824 51 21 0 151 201 67 117 8.15e-12 63.2
MS.gene034537.t1 MTR_3g086790 40.196 102 59 2 101 201 10 110 9.75e-12 62.8
MS.gene034537.t1 MTR_8g469430 54.902 51 23 0 151 201 89 139 4.18e-11 61.2
MS.gene034537.t1 MTR_5g039000 50.847 59 29 0 143 201 69 127 4.35e-11 60.8
MS.gene034537.t1 MTR_7g103340 48.276 58 30 0 144 201 56 113 5.20e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034537.t1 AT5G06710 41.406 256 79 7 1 201 1 240 7.50e-40 137
MS.gene034537.t1 AT5G06710 41.406 256 79 7 1 201 1 240 2.98e-39 137
MS.gene034537.t1 AT5G47370 48.795 166 66 8 48 201 22 180 1.76e-34 124
MS.gene034537.t1 AT4G17460 44.262 183 55 8 48 201 21 185 4.64e-30 112
MS.gene034537.t1 AT3G60390 51.429 140 46 6 81 201 76 212 5.64e-30 112
MS.gene034537.t1 AT4G16780 55.645 124 40 3 81 201 68 179 6.88e-28 106
MS.gene034537.t1 AT2G44910 48.905 137 47 4 81 201 84 213 9.67e-27 104
MS.gene034537.t1 AT2G22800 76.667 60 14 0 142 201 104 163 8.97e-25 98.2
MS.gene034537.t1 AT4G37790 76.667 60 14 0 142 201 117 176 2.55e-24 97.1
MS.gene034537.t1 AT5G06710 35.652 230 77 7 1 175 1 214 2.29e-20 85.9
MS.gene034537.t1 AT5G06710 35.652 230 77 7 1 175 1 214 2.29e-20 85.9
MS.gene034537.t1 AT2G01430 60.294 68 27 0 134 201 122 189 2.87e-20 85.1
MS.gene034537.t1 AT2G01430 60.294 68 27 0 134 201 122 189 9.28e-20 84.7
MS.gene034537.t1 AT1G70920 65.079 63 20 1 141 201 27 89 4.51e-17 75.5
MS.gene034537.t1 AT1G70920 65.079 63 20 1 141 201 57 119 7.70e-17 75.9
MS.gene034537.t1 AT2G36610 45.312 64 27 1 146 201 66 129 5.23e-11 59.7
MS.gene034537.t1 AT3G01470 56.863 51 22 0 151 201 68 118 6.77e-11 60.5

Find 0 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAATAAGAAATTTAC+AGG - 10640:4435-4454 MS.gene034537:intergenic 10.0%
!! AAATATATAAAACCATTTAG+AGG - 10640:3796-3815 MS.gene034537:intergenic 15.0%
!!! TGAAATGTCAATTTTTTTAA+CGG + 10640:3993-4012 MS.gene034537:intron 15.0%
!!! TTGTTTCTTCTTTTTAATTA+AGG + 10640:4467-4486 MS.gene034537:intron 15.0%
!! ATAGTAGATAGAAAATTACA+TGG - 10640:3759-3778 MS.gene034537:intergenic 20.0%
!!! TTTTTGTTTTTGTTATCATC+AGG + 10640:4120-4139 MS.gene034537:intron 20.0%
! AGATAGAAAATTACATGGTT+GGG - 10640:3754-3773 MS.gene034537:intergenic 25.0%
! TAGATAGAAAATTACATGGT+TGG - 10640:3755-3774 MS.gene034537:intergenic 25.0%
!! AGAAAAGAGATAGGTAATTT+TGG - 10640:3561-3580 MS.gene034537:intergenic 25.0%
!! ATCATTTTCTTTCCTCTAAA+TGG + 10640:3781-3800 MS.gene034537:intron 25.0%
!! TTAAAGTGCTTGAAAATTGT+GGG + 10640:3896-3915 MS.gene034537:intron 25.0%
!! TTTAAAGTGCTTGAAAATTG+TGG + 10640:3895-3914 MS.gene034537:intron 25.0%
!!! TCAATTTTTTTAACGGATCT+AGG + 10640:4000-4019 MS.gene034537:intron 25.0%
AAAACCTTGAACCAAATCTT+GGG - 10640:3934-3953 MS.gene034536:intergenic 30.0%
AAAGCTTCAGAAGAAAAAAG+AGG + 10640:3633-3652 MS.gene034537:CDS 30.0%
AATAACAATGCTGAATATGG+AGG + 10640:4267-4286 MS.gene034537:CDS 30.0%
AGAAACAAGAGAGAAAATGA+AGG + 10640:4291-4310 MS.gene034537:CDS 30.0%
AGGAATAACAATGCTGAATA+TGG + 10640:4264-4283 MS.gene034537:CDS 30.0%
ATACTGAAATCCATTGCAAA+TGG - 10640:4243-4262 MS.gene034537:intergenic 30.0%
GAAATTTACAGGATTAAGAG+TGG - 10640:4424-4443 MS.gene034537:intergenic 30.0%
! GAAGTGATGATGATGAAAAT+GGG + 10640:4328-4347 MS.gene034537:CDS 30.0%
!!! TTGTTTTTGTTATCATCAGG+TGG + 10640:4123-4142 MS.gene034537:intron 30.0%
ATGATGAAAATGGGTCAACT+AGG + 10640:4337-4356 MS.gene034537:CDS 35.0%
CAAAACCTTGAACCAAATCT+TGG - 10640:3935-3954 MS.gene034537:intergenic 35.0%
GGTTTGTCAAGAAAAGAGAT+AGG - 10640:3570-3589 MS.gene034537:intergenic 35.0%
TTGAATTTGCACCCAAGATT+TGG + 10640:3920-3939 MS.gene034537:intron 35.0%
! GGAAGTGATGATGATGAAAA+TGG + 10640:4327-4346 MS.gene034537:CDS 35.0%
! TTGCAATGGATTTCAGTATG+AGG + 10640:4244-4263 MS.gene034537:CDS 35.0%
!!! TTCTGAAGCTTTTCCTTGTT+GGG - 10640:3625-3644 MS.gene034537:intergenic 35.0%
AACCAAACTTCAACTTGACG+AGG - 10640:4572-4591 MS.gene034537:intergenic 40.0%
AATGAAGGTGAAGCTGAAAG+AGG + 10640:4306-4325 MS.gene034537:CDS 40.0%
AGAAGGTCTAGTTGAATAGG+TGG - 10640:3669-3688 MS.gene034537:intergenic 40.0%
AGTTTCTTCTCAACCCAACA+AGG + 10640:3609-3628 MS.gene034537:CDS 40.0%
GATCTAAGAACAGGAGAGAA+AGG - 10640:3693-3712 MS.gene034537:intergenic 40.0%
GGAAGAAGGTCTAGTTGAAT+AGG - 10640:3672-3691 MS.gene034537:intergenic 40.0%
GTAGAAGCAAAAACTTGCAC+TGG + 10640:4526-4545 MS.gene034537:intron 40.0%
GTTGAGAAGAAACTTGGTAG+TGG - 10640:3603-3622 MS.gene034537:intergenic 40.0%
TTCTGTGTCACCATTTGCAA+TGG + 10640:4230-4249 MS.gene034537:CDS 40.0%
! ATGGAACTTGCTTTGAGCTT+AGG + 10640:3528-3547 MS.gene034537:CDS 40.0%
! TTTGTTATCATCAGGTGGAC+CGG + 10640:4128-4147 MS.gene034537:intron 40.0%
!! ACTTGGTAGTGGATCTTTGT+TGG - 10640:3592-3611 MS.gene034537:intergenic 40.0%
!! CTTGGTAGTGGATCTTTGTT+GGG - 10640:3591-3610 MS.gene034537:intergenic 40.0%
!! TGTTGGGTTGAGAAGAAACT+TGG - 10640:3609-3628 MS.gene034537:intergenic 40.0%
!!! CTTCTGAAGCTTTTCCTTGT+TGG - 10640:3626-3645 MS.gene034537:intergenic 40.0%
AAATGGTGACACAGAACTGC+TGG - 10640:4226-4245 MS.gene034537:intergenic 45.0%
AATGGTGACACAGAACTGCT+GGG - 10640:4225-4244 MS.gene034537:intergenic 45.0%
GAACAGGAGAGAAAGGAAGA+AGG - 10640:3686-3705 MS.gene034537:intergenic 45.0%
! TGCACCCAAGATTTGGTTCA+AGG + 10640:3927-3946 MS.gene034537:intron 45.0%
!! GTCCTCGTCAAGTTGAAGTT+TGG + 10640:4567-4586 MS.gene034537:CDS 45.0%
ATGGTGACACAGAACTGCTG+GGG - 10640:4224-4243 MS.gene034537:intergenic 50.0%
GAATGGGGAGGTGAGAAGAA+GGG - 10640:3722-3741 MS.gene034537:intergenic 50.0%
GGGTGAGGAGATCTAAGAAC+AGG - 10640:3702-3721 MS.gene034537:intergenic 50.0%
TTACATGGTTGGGTGAGCCA+AGG - 10640:3744-3763 MS.gene034537:intergenic 50.0%
! GCTAGACGTGAACCGTTTTC+CGG + 10640:4158-4177 MS.gene034537:CDS 50.0%
ACTGCTGGGGGATGACAATG+AGG - 10640:4211-4230 MS.gene034537:intergenic 55.0%
ATCATCAGGTGGACCGGTGA+GGG + 10640:4134-4153 MS.gene034537:intron 55.0%
CTTCTCACCTCCCCATTCCT+TGG + 10640:3724-3743 MS.gene034537:CDS 55.0%
GGAATGGGGAGGTGAGAAGA+AGG - 10640:3723-3742 MS.gene034537:intergenic 55.0%
GGTTGGGTGAGCCAAGGAAT+GGG - 10640:3738-3757 MS.gene034537:intergenic 55.0%
GTTGGGTGAGCCAAGGAATG+GGG - 10640:3737-3756 MS.gene034537:intergenic 55.0%
TATCATCAGGTGGACCGGTG+AGG + 10640:4133-4152 MS.gene034537:intron 55.0%
TGGTGACACAGAACTGCTGG+GGG - 10640:4223-4242 MS.gene034537:intergenic 55.0%
TGGTTGGGTGAGCCAAGGAA+TGG - 10640:3739-3758 MS.gene034537:intergenic 55.0%
! TTCACGTCTAGCTCCCTCAC+CGG - 10640:4150-4169 MS.gene034537:intergenic 55.0%
ACGACCGAAGACGTCGACGA+CGG + 10640:4183-4202 MS.gene034537:CDS 60.0%
CTTCGGTCGTCGCCGGAAAA+CGG - 10640:4173-4192 MS.gene034537:intergenic 60.0%
GGGAGGTGAGAAGAAGGGTG+AGG - 10640:3717-3736 MS.gene034537:intergenic 60.0%
GGTACCGTCGTCGACGTCTT+CGG - 10640:4190-4209 MS.gene034537:intergenic 60.0%
GGGTGAGCCAAGGAATGGGG+AGG - 10640:3734-3753 MS.gene034537:intergenic 65.0%
TCGACGTCTTCGGTCGTCGC+CGG - 10640:4180-4199 MS.gene034537:intergenic 65.0%


Chromosome Type Strat End Strand Name
10640 gene 3528 4609 3528 ID=MS.gene034537
10640 mRNA 3528 4609 3528 ID=MS.gene034537.t1;Parent=MS.gene034537
10640 exon 3528 3759 3528 ID=MS.gene034537.t1.exon1;Parent=MS.gene034537.t1
10640 CDS 3528 3759 3528 ID=cds.MS.gene034537.t1;Parent=MS.gene034537.t1
10640 exon 4142 4434 4142 ID=MS.gene034537.t1.exon2;Parent=MS.gene034537.t1
10640 CDS 4142 4434 4142 ID=cds.MS.gene034537.t1;Parent=MS.gene034537.t1
10640 exon 4530 4609 4530 ID=MS.gene034537.t1.exon3;Parent=MS.gene034537.t1
10640 CDS 4530 4609 4530 ID=cds.MS.gene034537.t1;Parent=MS.gene034537.t1
Gene Sequence

>MS.gene034537

ATGGAACTTGCTTTGAGCTTAGGTGACACACCAAAATTACCTATCTCTTTTCTTGACAAACCCAACAAAGATCCACTACCAAGTTTCTTCTCAACCCAACAAGGAAAAGCTTCAGAAGAAAAAAGAGGTTCTTCAGATCCACCTATTCAACTAGACCTTCTTCCTTTCTCTCCTGTTCTTAGATCTCCTCACCCTTCTTCTCACCTCCCCATTCCTTGGCTCACCCAACCATGTAATTTTCTATCTACTATAAATCATTTTCTTTCCTCTAAATGGTTTTATATATTTGTTTAATATCTAGATAAATTTGTTTAATTTTATTTTGTTTTTATAGATTTAATTTTATCTTTTTTGTTTTTTTTTTTGTTTTAAAGTGCTTGAAAATTGTGGGTTTGAATTTGCACCCAAGATTTGGTTCAAGGTTTTGCATATTCTTGACTAATGCTTATTGAAAAATATTTGTTATGAAATGTCAATTTTTTTAACGGATCTAGGTTACTTCGAGATATTAGTCTTTGCAGTTACGCGCATGAATTCATATTGTTATAAAAAAAAATTGAAAATATATTTAAAGTTGTATATACTCAATCATTTTTTGTTTTTGTTATCATCAGGTGGACCGGTGAGGGAGCTAGACGTGAACCGTTTTCCGGCGACGACCGAAGACGTCGACGACGGTACCTCATTGTCATCCCCCAGCAGTTCTGTGTCACCATTTGCAATGGATTTCAGTATGAGGAATAACAATGCTGAATATGGAGGAAGAAACAAGAGAGAAAATGAAGGTGAAGCTGAAAGAGGAAGTGATGATGATGAAAATGGGTCAACTAGGAAGAAACTAAGACTCTCTAAAGATCAATCTGCTTTTCTTGAAGAAAGTTTCAAAGAACACACCACTCTTAATCCTGTAAATTTCTTATTTAATTTTTGTTTATTTACTTGTTTCTTCTTTTTAATTAAGGTTTATTTGTTAATTAATCATGTTTATTTATTTGCTTGTAGAAGCAAAAACTTGCACTGGCAAAACAGTTGAATCTTAGTCCTCGTCAAGTTGAAGTTTGGTTTCAAAATAGAAGAGCAAG

Protein sequence

>MS.gene034537.t1

MELALSLGDTPKLPISFLDKPNKDPLPSFFSTQQGKASEEKRGSSDPPIQLDLLPFSPVLRSPHPSSHLPIPWLTQPCGPVRELDVNRFPATTEDVDDGTSLSSPSSSVSPFAMDFSMRNNNAEYGGRNKRENEGEAERGSDDDENGSTRKKLRLSKDQSAFLEESFKEHTTLNPKQKLALAKQLNLSPRQVEVWFQNRRA