Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034562.t1 | XP_004513765.1 | 87 | 54 | 7 | 0 | 1 | 54 | 195 | 248 | 5.30E-18 | 100.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034562.t1 | Q8VZ42 | 52.7 | 55 | 21 | 1 | 1 | 55 | 190 | 239 | 3.3e-08 | 58.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034562.t1 | A0A392R514 | 89.5 | 57 | 6 | 0 | 1 | 57 | 59 | 115 | 1.1e-20 | 108.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene034562.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034562.t1 | MTR_0100s0160 | 91.489 | 94 | 2 | 1 | 1 | 88 | 193 | 286 | 1.32e-55 | 173 |
| MS.gene034562.t1 | MTR_7g091810 | 60.563 | 71 | 20 | 1 | 25 | 87 | 198 | 268 | 3.48e-23 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034562.t1 | AT2G41835 | 51.064 | 94 | 35 | 2 | 1 | 88 | 190 | 278 | 4.02e-26 | 97.4 |
Find 25 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTAGTGATTGAGGAAAACT+TGG | 0.473429 | 1.1:-41421798 | None:intergenic |
| CACAGATCCCGTGTCCCTTG+TGG | 0.520415 | 1.1:+41421898 | MS.gene034562:CDS |
| GCGTGTCCTAAGTGTAGTAA+AGG | 0.526636 | 1.1:+41421872 | MS.gene034562:CDS |
| CCCTCTCAACATGCTCCACA+AGG | 0.529390 | 1.1:-41421913 | None:intergenic |
| AAAACTTGGCACCACATTGA+GGG | 0.531828 | 1.1:-41421784 | None:intergenic |
| GTGAATCCTTTACTACACTT+AGG | 0.538962 | 1.1:-41421878 | None:intergenic |
| ATCGATTAAAGTAGTGATTG+AGG | 0.539536 | 1.1:-41421807 | None:intergenic |
| GTGGTGAAATTAACCAAGCA+TGG | 0.560055 | 1.1:+41421705 | MS.gene034562:CDS |
| TGGTGAAATTAACCAAGCAT+GGG | 0.560973 | 1.1:+41421706 | MS.gene034562:CDS |
| GAAAACTTGGCACCACATTG+AGG | 0.568886 | 1.1:-41421785 | None:intergenic |
| AACATGCTCCACAAGGGACA+CGG | 0.573079 | 1.1:-41421906 | None:intergenic |
| TGGTGGCACTTCTAGAACAT+AGG | 0.575559 | 1.1:+41421940 | MS.gene034562:CDS |
| GAGCAACGACAGTGGTCAAG+TGG | 0.579833 | 1.1:+41421754 | MS.gene034562:CDS |
| ACATGCTCCACAAGGGACAC+GGG | 0.604172 | 1.1:-41421905 | None:intergenic |
| ACTTAGGACACGCATCAGAT+CGG | 0.613401 | 1.1:-41421862 | None:intergenic |
| GAGCATGTTGAGAGGGAACA+TGG | 0.625868 | 1.1:+41421920 | MS.gene034562:CDS |
| CCCTTGTGGAGCATGTTGAG+AGG | 0.626858 | 1.1:+41421912 | MS.gene034562:CDS |
| CCTTGTGGAGCATGTTGAGA+GGG | 0.637426 | 1.1:+41421913 | MS.gene034562:CDS |
| GGGAGAAAGAGCAACGACAG+TGG | 0.676386 | 1.1:+41421746 | MS.gene034562:CDS |
| GGTGAAATTAACCAAGCATG+GGG | 0.687152 | 1.1:+41421707 | MS.gene034562:CDS |
| CCTCTCAACATGCTCCACAA+GGG | 0.702395 | 1.1:-41421912 | None:intergenic |
| GCTGGAATGTCAAAGTTGAG+TGG | 0.723894 | 1.1:+41421686 | None:intergenic |
| GTGAAATTAACCAAGCATGG+GGG | 0.745364 | 1.1:+41421708 | MS.gene034562:CDS |
| CATGTTGAGAGGGAACATGG+TGG | 0.749762 | 1.1:+41421923 | MS.gene034562:CDS |
| GTGGAGCAATGCCCTCAATG+TGG | 0.769864 | 1.1:+41421773 | MS.gene034562:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCGATTAAAGTAGTGATTG+AGG | - | chr1.1:41421810-41421829 | None:intergenic | 30.0% | |
| CAAAAAGTTCATGAAAGAAG+TGG | + | chr1.1:41421836-41421855 | MS.gene034562:CDS | 30.0% | |
| AGTAGTGATTGAGGAAAACT+TGG | - | chr1.1:41421801-41421820 | None:intergenic | 35.0% | |
| GTGAATCCTTTACTACACTT+AGG | - | chr1.1:41421881-41421900 | None:intergenic | 35.0% | |
| TGGTGAAATTAACCAAGCAT+GGG | + | chr1.1:41421706-41421725 | MS.gene034562:CDS | 35.0% | |
| AAAACTTGGCACCACATTGA+GGG | - | chr1.1:41421787-41421806 | None:intergenic | 40.0% | |
| GGTGAAATTAACCAAGCATG+GGG | + | chr1.1:41421707-41421726 | MS.gene034562:CDS | 40.0% | |
| GTGAAATTAACCAAGCATGG+GGG | + | chr1.1:41421708-41421727 | MS.gene034562:CDS | 40.0% | |
| GTGGTGAAATTAACCAAGCA+TGG | + | chr1.1:41421705-41421724 | MS.gene034562:CDS | 40.0% | |
| ACTTAGGACACGCATCAGAT+CGG | - | chr1.1:41421865-41421884 | None:intergenic | 45.0% | |
| GAAAACTTGGCACCACATTG+AGG | - | chr1.1:41421788-41421807 | None:intergenic | 45.0% | |
| GCGTGTCCTAAGTGTAGTAA+AGG | + | chr1.1:41421872-41421891 | MS.gene034562:CDS | 45.0% | |
| AACATGCTCCACAAGGGACA+CGG | - | chr1.1:41421909-41421928 | None:intergenic | 50.0% | |
| CATGTTGAGAGGGAACATGG+TGG | + | chr1.1:41421923-41421942 | MS.gene034562:CDS | 50.0% | |
| CCTCTCAACATGCTCCACAA+GGG | - | chr1.1:41421915-41421934 | None:intergenic | 50.0% | |
| CCTTGTGGAGCATGTTGAGA+GGG | + | chr1.1:41421913-41421932 | MS.gene034562:CDS | 50.0% | |
| GAGCATGTTGAGAGGGAACA+TGG | + | chr1.1:41421920-41421939 | MS.gene034562:CDS | 50.0% | |
| ACATGCTCCACAAGGGACAC+GGG | - | chr1.1:41421908-41421927 | None:intergenic | 55.0% | |
| CCCTCTCAACATGCTCCACA+AGG | - | chr1.1:41421916-41421935 | None:intergenic | 55.0% | |
| CCCTTGTGGAGCATGTTGAG+AGG | + | chr1.1:41421912-41421931 | MS.gene034562:CDS | 55.0% | |
| GAGCAACGACAGTGGTCAAG+TGG | + | chr1.1:41421754-41421773 | MS.gene034562:CDS | 55.0% | |
| GGGAGAAAGAGCAACGACAG+TGG | + | chr1.1:41421746-41421765 | MS.gene034562:CDS | 55.0% | |
| GTGGAGCAATGCCCTCAATG+TGG | + | chr1.1:41421773-41421792 | MS.gene034562:CDS | 55.0% | |
| CACAGATCCCGTGTCCCTTG+TGG | + | chr1.1:41421898-41421917 | MS.gene034562:CDS | 60.0% | |
| CGCTTGATGTCCCCCATGCT+TGG | - | chr1.1:41421721-41421740 | None:intergenic | 60.0% | |
| GCATGGGGGACATCAAGCGA+TGG | + | chr1.1:41421722-41421741 | MS.gene034562:CDS | 60.0% | |
| GGGGGACATCAAGCGATGGT+GGG | + | chr1.1:41421726-41421745 | MS.gene034562:CDS | 60.0% | |
| TGGGGGACATCAAGCGATGG+TGG | + | chr1.1:41421725-41421744 | MS.gene034562:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 41421692 | 41421961 | 41421692 | ID=MS.gene034562 |
| chr1.1 | mRNA | 41421692 | 41421961 | 41421692 | ID=MS.gene034562.t1;Parent=MS.gene034562 |
| chr1.1 | exon | 41421692 | 41421961 | 41421692 | ID=MS.gene034562.t1.exon1;Parent=MS.gene034562.t1 |
| chr1.1 | CDS | 41421692 | 41421961 | 41421692 | ID=cds.MS.gene034562.t1;Parent=MS.gene034562.t1 |
| Gene Sequence |
| Protein sequence |
