Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034811.t1 | XP_013441486.1 | 91.8 | 182 | 13 | 1 | 1 | 180 | 1 | 182 | 2.60E-67 | 265 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034811.t1 | Q3EAL1 | 42.5 | 193 | 64 | 4 | 1 | 184 | 1 | 155 | 1.6e-28 | 127.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034811.t1 | A0A072TE55 | 91.8 | 182 | 13 | 1 | 1 | 180 | 1 | 182 | 1.9e-67 | 265.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene034811.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034811.t1 | MTR_0907s0020 | 91.209 | 182 | 14 | 1 | 1 | 180 | 1 | 182 | 1.09e-105 | 301 |
| MS.gene034811.t1 | MTR_7g105790 | 58.947 | 190 | 65 | 4 | 1 | 179 | 1 | 188 | 3.48e-63 | 194 |
| MS.gene034811.t1 | MTR_4g052040 | 36.667 | 180 | 93 | 4 | 1 | 170 | 1 | 169 | 7.00e-26 | 98.2 |
| MS.gene034811.t1 | MTR_5g079710 | 39.024 | 164 | 89 | 3 | 5 | 168 | 7 | 159 | 1.74e-25 | 99.0 |
| MS.gene034811.t1 | MTR_4g052060 | 37.059 | 170 | 92 | 4 | 1 | 166 | 1 | 159 | 8.07e-23 | 90.1 |
| MS.gene034811.t1 | MTR_8g103520 | 54.167 | 72 | 32 | 1 | 95 | 166 | 220 | 290 | 2.60e-19 | 83.2 |
| MS.gene034811.t1 | MTR_5g075210 | 53.226 | 62 | 29 | 0 | 100 | 161 | 280 | 341 | 8.57e-17 | 77.0 |
| MS.gene034811.t1 | MTR_4g028930 | 44.928 | 69 | 38 | 0 | 93 | 161 | 307 | 375 | 7.50e-16 | 74.7 |
| MS.gene034811.t1 | MTR_7g072875 | 31.737 | 167 | 83 | 8 | 22 | 161 | 118 | 280 | 7.64e-15 | 71.2 |
| MS.gene034811.t1 | MTR_7g072630 | 31.737 | 167 | 83 | 8 | 22 | 161 | 180 | 342 | 1.89e-14 | 70.5 |
| MS.gene034811.t1 | MTR_3g111780 | 56.061 | 66 | 29 | 0 | 100 | 165 | 198 | 263 | 4.30e-14 | 68.9 |
| MS.gene034811.t1 | MTR_8g087530 | 48.438 | 64 | 33 | 0 | 98 | 161 | 256 | 319 | 9.84e-14 | 68.6 |
| MS.gene034811.t1 | MTR_2g018030 | 38.961 | 77 | 43 | 1 | 103 | 175 | 280 | 356 | 8.47e-13 | 65.9 |
| MS.gene034811.t1 | MTR_4g132740 | 42.424 | 66 | 38 | 0 | 102 | 167 | 83 | 148 | 6.28e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034811.t1 | AT3G52525 | 43.523 | 193 | 62 | 5 | 1 | 184 | 1 | 155 | 2.31e-39 | 132 |
| MS.gene034811.t1 | AT2G36026 | 44.099 | 161 | 71 | 2 | 8 | 167 | 11 | 153 | 2.53e-34 | 120 |
| MS.gene034811.t1 | AT2G18500 | 54.412 | 68 | 31 | 0 | 97 | 164 | 223 | 290 | 9.89e-21 | 87.4 |
| MS.gene034811.t1 | AT5G19650 | 52.239 | 67 | 32 | 0 | 100 | 166 | 154 | 220 | 1.15e-20 | 85.5 |
| MS.gene034811.t1 | AT1G06920 | 39.394 | 99 | 50 | 2 | 75 | 173 | 226 | 314 | 5.30e-16 | 74.7 |
| MS.gene034811.t1 | AT2G30400 | 51.613 | 62 | 30 | 0 | 100 | 161 | 252 | 313 | 2.32e-15 | 72.8 |
| MS.gene034811.t1 | AT5G01840 | 53.448 | 58 | 27 | 0 | 104 | 161 | 202 | 259 | 2.78e-14 | 69.3 |
| MS.gene034811.t1 | AT4G18830 | 47.059 | 68 | 36 | 0 | 95 | 162 | 277 | 344 | 4.09e-14 | 69.7 |
| MS.gene034811.t1 | AT5G58360 | 47.458 | 59 | 31 | 0 | 103 | 161 | 227 | 285 | 4.37e-14 | 68.9 |
| MS.gene034811.t1 | AT5G22240 | 43.750 | 64 | 36 | 0 | 99 | 162 | 95 | 158 | 5.46e-13 | 64.7 |
Find 0 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGAACTAGAATTGTTATTA+AGG | - | 8685:174691-174710 | None:intergenic | 20.0% |
| ! | AATGTCGAAAATCAAGATAA+GGG | - | 8685:174874-174893 | None:intergenic | 25.0% |
| !! | AAATCATCTTTGAAAGGATT+TGG | + | 8685:174803-174822 | MS.gene034811:CDS | 25.0% |
| !! | TTGAATATGAAACCAAGTTT+TGG | - | 8685:174617-174636 | None:intergenic | 25.0% |
| !!! | GTTTAAGGTTAGTTTTTTTC+TGG | - | 8685:174555-174574 | None:intergenic | 25.0% |
| !!! | TATTAAGGTGATGTTTTTTG+TGG | - | 8685:174676-174695 | None:intergenic | 25.0% |
| GAATGTCGAAAATCAAGATA+AGG | - | 8685:174875-174894 | None:intergenic | 30.0% | |
| TCAGAGAAATCATCTTTGAA+AGG | + | 8685:174797-174816 | MS.gene034811:CDS | 30.0% | |
| TTCAATGTTGCAGATGATAT+TGG | + | 8685:174892-174911 | MS.gene034811:CDS | 30.0% | |
| ! | TTAGAGCTTTTACTGAGATT+TGG | + | 8685:175002-175021 | MS.gene034811:CDS | 30.0% |
| !! | ACAATTCTAGTTCAACCATT+TGG | + | 8685:174696-174715 | MS.gene034811:CDS | 30.0% |
| !!! | TAAGGTTAGTTTTTTTCTGG+AGG | - | 8685:174552-174571 | None:intergenic | 30.0% |
| !!! | TTAAGTCTGGTTTTTCATCT+GGG | + | 8685:175041-175060 | MS.gene034811:CDS | 30.0% |
| !!! | TTTTTGTGGTTTTGGTAAGT+GGG | - | 8685:174662-174681 | None:intergenic | 30.0% |
| !!! | TTTTTTGTGGTTTTGGTAAG+TGG | - | 8685:174663-174682 | None:intergenic | 30.0% |
| ACATGTACAAGACCAAAACT+TGG | + | 8685:174602-174621 | MS.gene034811:CDS | 35.0% | |
| GTTTATGGAGACAGTGTTTA+AGG | - | 8685:174570-174589 | None:intergenic | 35.0% | |
| TCTGTCTTTCTGTTTCCAAA+TGG | - | 8685:174714-174733 | None:intergenic | 35.0% | |
| TGAATTCGAAGGAACATCAT+GGG | + | 8685:174972-174991 | MS.gene034811:CDS | 35.0% | |
| TTGAAAGGATTTGGAAGAGT+TGG | + | 8685:174812-174831 | MS.gene034811:CDS | 35.0% | |
| TTTGGAAGAGTTGGAAATGA+AGG | + | 8685:174821-174840 | MS.gene034811:CDS | 35.0% | |
| ! | GCTTTTACTGAGATTTGGAA+TGG | + | 8685:175007-175026 | MS.gene034811:CDS | 35.0% |
| ! | TAAGTGGGGTTTTTGTGTTT+TGG | - | 8685:174647-174666 | None:intergenic | 35.0% |
| ! | TTTTCTTCAGCTGAATTCGA+AGG | + | 8685:174961-174980 | MS.gene034811:CDS | 35.0% |
| !!! | GGTGATGTTTTTTGTGGTTT+TGG | - | 8685:174670-174689 | None:intergenic | 35.0% |
| !!! | GTTAAGTCTGGTTTTTCATC+TGG | + | 8685:175040-175059 | MS.gene034811:CDS | 35.0% |
| !!! | TTTTGTGGTTTTGGTAAGTG+GGG | - | 8685:174661-174680 | None:intergenic | 35.0% |
| AACACTGTCTCCATAAACCT+TGG | + | 8685:174572-174591 | MS.gene034811:CDS | 40.0% | |
| CCGAATGATGTGTTTGTTCT+CGG | - | 8685:174776-174795 | None:intergenic | 40.0% | |
| CCGAGAACAAACACATCATT+CGG | + | 8685:174773-174792 | MS.gene034811:CDS | 40.0% | |
| CTGAATTCGAAGGAACATCA+TGG | + | 8685:174971-174990 | MS.gene034811:CDS | 40.0% | |
| TGTTCCACAACCAAGGTTTA+TGG | - | 8685:174585-174604 | None:intergenic | 40.0% | |
| TTGTACATGTTCCACAACCA+AGG | - | 8685:174592-174611 | None:intergenic | 40.0% | |
| ! | GGAGTTTTCTCTGTTAAGTC+TGG | + | 8685:175028-175047 | MS.gene034811:CDS | 40.0% |
| GTCTCCATAAACCTTGGTTG+TGG | + | 8685:174578-174597 | MS.gene034811:CDS | 45.0% | |
| ! | TGGAAATGAAGGTGTAGCTG+TGG | + | 8685:174832-174851 | MS.gene034811:CDS | 45.0% |
| !! | GTTTGTTCTCGGTGTTGTGT+TGG | - | 8685:174765-174784 | None:intergenic | 45.0% |
| !! | CGGTGTTGTGTTGGTTGTGC+TGG | - | 8685:174756-174775 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8685 | gene | 174542 | 175096 | 174542 | ID=MS.gene034811 |
| 8685 | mRNA | 174542 | 175096 | 174542 | ID=MS.gene034811.t1;Parent=MS.gene034811 |
| 8685 | exon | 174542 | 175096 | 174542 | ID=MS.gene034811.t1.exon1;Parent=MS.gene034811.t1 |
| 8685 | CDS | 174542 | 175096 | 174542 | ID=cds.MS.gene034811.t1;Parent=MS.gene034811.t1 |
| Gene Sequence |
| Protein sequence |
