Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034813.t1 | XP_013467912.2 | 93 | 230 | 16 | 0 | 1 | 230 | 1 | 230 | 7.70E-112 | 413.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034813.t1 | Q9FMC8 | 31.6 | 256 | 129 | 9 | 3 | 230 | 4 | 241 | 1.5e-18 | 94.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034813.t1 | A0A396JMH3 | 93.0 | 230 | 16 | 0 | 1 | 230 | 1 | 230 | 5.6e-112 | 413.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene034813.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034813.t1 | MTR_1g058000 | 93.363 | 226 | 15 | 0 | 5 | 230 | 1 | 226 | 1.97e-130 | 367 |
| MS.gene034813.t1 | MTR_7g105780 | 45.771 | 201 | 93 | 7 | 3 | 195 | 5 | 197 | 1.77e-43 | 148 |
| MS.gene034813.t1 | MTR_1g111640 | 41.232 | 211 | 91 | 6 | 3 | 195 | 4 | 199 | 1.84e-42 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034813.t1 | AT5G04820 | 33.333 | 246 | 136 | 8 | 3 | 225 | 4 | 244 | 4.14e-30 | 113 |
| MS.gene034813.t1 | AT2G36050 | 36.994 | 173 | 75 | 8 | 27 | 195 | 29 | 171 | 4.91e-15 | 72.8 |
| MS.gene034813.t1 | AT3G52540 | 39.316 | 117 | 56 | 5 | 82 | 195 | 94 | 198 | 1.23e-11 | 63.5 |
Find 0 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAAATATTAATTATTTCTT+TGG | + | 8685:202523-202542 | None:intergenic | 10.0% |
| !! | ATAAGAAAATTCAGTTAATA+TGG | + | 8685:202394-202413 | None:intergenic | 15.0% |
| !! | GTAAGTTAAGGAATATTAAA+AGG | - | 8685:202890-202909 | MS.gene034813:CDS | 20.0% |
| !! | TAACAACTTATTATTACCAT+TGG | - | 8685:202569-202588 | MS.gene034813:CDS | 20.0% |
| !!! | ACTGTATTTTGTTGAAAAAA+TGG | - | 8685:202931-202950 | MS.gene034813:CDS | 20.0% |
| !!! | TTTTCTTATGAGTATTTCAA+AGG | - | 8685:202405-202424 | MS.gene034813:intron | 20.0% |
| ! | AACAAAGAAAACAAAACCAT+TGG | + | 8685:203093-203112 | None:intergenic | 25.0% |
| ! | AATATTAAAAGGTGGAGATT+GGG | - | 8685:202901-202920 | MS.gene034813:CDS | 25.0% |
| ! | AGTTAAGGAATATTAAAAGG+TGG | - | 8685:202893-202912 | MS.gene034813:CDS | 25.0% |
| ! | ATTCACTACTCAAAATCATT+TGG | + | 8685:202958-202977 | None:intergenic | 25.0% |
| ! | GAACTATAAGATGAAATACA+AGG | + | 8685:202864-202883 | None:intergenic | 25.0% |
| ! | GTTAATGAGAAAAGAAATCA+TGG | - | 8685:202726-202745 | MS.gene034813:CDS | 25.0% |
| ! | TAATATTCCTTAACTTACCA+AGG | + | 8685:202888-202907 | None:intergenic | 25.0% |
| !! | TCTCTTTTCAAAAACAAAGA+TGG | - | 8685:202222-202241 | MS.gene034813:intron | 25.0% |
| !!! | TAAAAGAAAGTGTTTTTGGT+TGG | + | 8685:202284-202303 | None:intergenic | 25.0% |
| AGACTTACAAATTCTTCCAA+TGG | - | 8685:203074-203093 | MS.gene034813:CDS | 30.0% | |
| AGATTTCAAAGATTCTATCG+TGG | - | 8685:202635-202654 | MS.gene034813:CDS | 30.0% | |
| GAATATTAAAAGGTGGAGAT+TGG | - | 8685:202900-202919 | MS.gene034813:CDS | 30.0% | |
| GAGTATTTCAAAGGTAATAG+TGG | - | 8685:202414-202433 | MS.gene034813:intron | 30.0% | |
| TCAATAGAGACAATGATTTG+CGG | - | 8685:202441-202460 | MS.gene034813:intron | 30.0% | |
| TTTCATCTTATAGTTCACCT+TGG | - | 8685:202868-202887 | MS.gene034813:CDS | 30.0% | |
| ! | AATATCGAGCTTGTTTCATT+CGG | + | 8685:202495-202514 | None:intergenic | 30.0% |
| !! | AGTTTCTAGGATATCTTTGT+CGG | + | 8685:202364-202383 | None:intergenic | 30.0% |
| !! | GAATCTTTGAAATCTTCACA+AGG | + | 8685:202630-202649 | None:intergenic | 30.0% |
| !!! | AAAGCTTTTGAGTTGGTATT+TGG | - | 8685:202701-202720 | MS.gene034813:CDS | 30.0% |
| !!! | GCTCTAAAAGAAAGTGTTTT+TGG | + | 8685:202288-202307 | None:intergenic | 30.0% |
| ATATGGAGTCAGAAGTTTCT+AGG | + | 8685:202377-202396 | None:intergenic | 35.0% | |
| ATGGAAAATCCTCTTCTTCA+TGG | - | 8685:202241-202260 | MS.gene034813:intron | 35.0% | |
| CAAAGATTCTATCGTGGAAA+TGG | - | 8685:202641-202660 | MS.gene034813:CDS | 35.0% | |
| CGAATCCATTGAAAACTCAA+GGG | + | 8685:202601-202620 | None:intergenic | 35.0% | |
| GTTCTTCTCTTGATTCATTG+TGG | - | 8685:202832-202851 | MS.gene034813:CDS | 35.0% | |
| TAGTTCACCTTGGTAAGTTA+AGG | - | 8685:202878-202897 | MS.gene034813:CDS | 35.0% | |
| ! | AATGATTTGCGGTTTGTGTT+CGG | - | 8685:202452-202471 | MS.gene034813:intron | 35.0% |
| ! | ACCAACTCAAAAGCTTTTCT+AGG | + | 8685:202698-202717 | None:intergenic | 35.0% |
| ! | CCAACTCAAAAGCTTTTCTA+GGG | + | 8685:202697-202716 | None:intergenic | 35.0% |
| !! | TGGGTTGATGGATTTGAATA+CGG | + | 8685:202325-202344 | None:intergenic | 35.0% |
| !!! | GTTTTTGGTTGGTTACAAGA+TGG | + | 8685:202273-202292 | None:intergenic | 35.0% |
| AATCCTCTTCTTCATGGTCT+TGG | - | 8685:202247-202266 | MS.gene034813:intron | 40.0% | |
| AGATTCTATCGTGGAAATGG+TGG | - | 8685:202644-202663 | MS.gene034813:CDS | 40.0% | |
| GCGAATCCATTGAAAACTCA+AGG | + | 8685:202602-202621 | None:intergenic | 40.0% | |
| ! | GAACACCCTTGAGTTTTCAA+TGG | - | 8685:202593-202612 | MS.gene034813:CDS | 40.0% |
| ! | TCCATCAACCCAACTTTTGT+AGG | - | 8685:202333-202352 | MS.gene034813:intron | 40.0% |
| !! | ACCTACAAAAGTTGGGTTGA+TGG | + | 8685:202337-202356 | None:intergenic | 40.0% |
| !! | CCCTAGAAAAGCTTTTGAGT+TGG | - | 8685:202694-202713 | MS.gene034813:CDS | 40.0% |
| !! | TTCTAGGATATCTTTGTCGG+TGG | + | 8685:202361-202380 | None:intergenic | 40.0% |
| !!! | GAATACGGTGTCGTTTTTGT+TGG | + | 8685:202310-202329 | None:intergenic | 40.0% |
| AGGCTCATGGCATTAACGAT+TGG | - | 8685:202667-202686 | MS.gene034813:CDS | 45.0% | |
| CGGTGGAACCTACAAAAGTT+GGG | + | 8685:202344-202363 | None:intergenic | 45.0% | |
| GGCTCATGGCATTAACGATT+GGG | - | 8685:202668-202687 | MS.gene034813:CDS | 45.0% | |
| TCGGTGGAACCTACAAAAGT+TGG | + | 8685:202345-202364 | None:intergenic | 45.0% | |
| TTCTATCGTGGAAATGGTGG+AGG | - | 8685:202647-202666 | MS.gene034813:CDS | 45.0% | |
| TTTGCGGTTTGTGTTCGGAT+AGG | - | 8685:202457-202476 | MS.gene034813:intron | 45.0% | |
| !! | AACTCAAGGGTGTTCTCCAA+TGG | + | 8685:202588-202607 | None:intergenic | 45.0% |
| TGGCCAAGACCATGAAGAAG+AGG | + | 8685:202253-202272 | None:intergenic | 50.0% | |
| ! | GTGGAAATGGTGGAGGCTCA+TGG | - | 8685:202654-202673 | MS.gene034813:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8685 | gene | 202198 | 203163 | 202198 | ID=MS.gene034813 |
| 8685 | mRNA | 202198 | 203163 | 202198 | ID=MS.gene034813.t1;Parent=MS.gene034813 |
| 8685 | exon | 202472 | 203163 | 202472 | ID=MS.gene034813.t1.exon1;Parent=MS.gene034813.t1 |
| 8685 | CDS | 202472 | 203163 | 202472 | ID=cds.MS.gene034813.t1;Parent=MS.gene034813.t1 |
| 8685 | exon | 202198 | 202234 | 202198 | ID=MS.gene034813.t1.exon2;Parent=MS.gene034813.t1 |
| 8685 | CDS | 202198 | 202234 | 202198 | ID=cds.MS.gene034813.t1;Parent=MS.gene034813.t1 |
| Gene Sequence |
| Protein sequence |
