Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034817.t1 | KEH41952.1 | 92.3 | 91 | 5 | 1 | 1 | 89 | 1 | 91 | 1.60E-38 | 168.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034817.t1 | A0A072VJI7 | 92.3 | 91 | 5 | 1 | 1 | 89 | 1 | 91 | 1.2e-38 | 168.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene034817.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034817.t1 | MTR_1g058090 | 92.308 | 91 | 5 | 1 | 1 | 89 | 1 | 91 | 6.91e-58 | 172 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGTAGTGATCATTTCTTTCA+TGG | - | 8685:230906-230925 | None:intergenic | 30.0% | |
| !! | ATTGATTTGGAACTAAGACT+TGG | + | 8685:230992-231011 | MS.gene034817:CDS | 30.0% |
| AGGAGGAGAAGAAATTGATT+TGG | + | 8685:230979-230998 | MS.gene034817:CDS | 35.0% | |
| CATTCACTCTTGAAAAAGTC+AGG | - | 8685:230838-230857 | None:intergenic | 35.0% | |
| ATTTCTTCTCCTCCTTGACT+TGG | - | 8685:230974-230993 | None:intergenic | 40.0% | |
| TCAAGAGTGAATGCCTTGAA+TGG | + | 8685:230845-230864 | MS.gene034817:CDS | 40.0% | |
| !!! | GACTTGGATTGGTGACTTTT+TGG | - | 8685:230952-230971 | None:intergenic | 40.0% |
| AATCCAAGTCCAAGTCAAGG+AGG | + | 8685:230962-230981 | MS.gene034817:CDS | 45.0% | |
| ACCAATCCAAGTCCAAGTCA+AGG | + | 8685:230959-230978 | MS.gene034817:CDS | 45.0% | |
| CACTACTCTCAACCAACATG+AGG | + | 8685:230919-230938 | MS.gene034817:CDS | 45.0% | |
| CCACAGGGAGGAGAAAAATA+AGG | + | 8685:230880-230899 | MS.gene034817:CDS | 45.0% | |
| GACTCTCATGTGACCATTCA+AGG | - | 8685:230861-230880 | None:intergenic | 45.0% | |
| TCTAGGTCCAACTTAGTGCT+AGG | - | 8685:231034-231053 | None:intergenic | 45.0% | |
| ! | ACTAAGACTTGGTCCAACCA+AGG | + | 8685:231003-231022 | MS.gene034817:CDS | 45.0% |
| !! | TCCTTGACTTGGACTTGGAT+TGG | - | 8685:230963-230982 | None:intergenic | 45.0% |
| !! | TTTGGTTCTTCGCCTCATGT+TGG | - | 8685:230934-230953 | None:intergenic | 45.0% |
| !!! | CCTTATTTTTCTCCTCCCTG+TGG | - | 8685:230883-230902 | None:intergenic | 45.0% |
| ATGGTCACATGAGAGTCCAC+AGG | + | 8685:230864-230883 | MS.gene034817:CDS | 50.0% | |
| CTTAGTGCTAGGAGTAGCCT+TGG | - | 8685:231023-231042 | None:intergenic | 50.0% | |
| GGCTACTCCTAGCACTAAGT+TGG | + | 8685:231024-231043 | MS.gene034817:CDS | 50.0% | |
| TGGTCACATGAGAGTCCACA+GGG | + | 8685:230865-230884 | MS.gene034817:CDS | 50.0% | |
| !! | TCTCCTCCTTGACTTGGACT+TGG | - | 8685:230968-230987 | None:intergenic | 50.0% |
| TCACATGAGAGTCCACAGGG+AGG | + | 8685:230868-230887 | MS.gene034817:CDS | 55.0% | |
| ! | GTGCTAGGAGTAGCCTTGGT+TGG | - | 8685:231019-231038 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8685 | gene | 230797 | 231066 | 230797 | ID=MS.gene034817 |
| 8685 | mRNA | 230797 | 231066 | 230797 | ID=MS.gene034817.t1;Parent=MS.gene034817 |
| 8685 | exon | 230797 | 231066 | 230797 | ID=MS.gene034817.t1.exon1;Parent=MS.gene034817.t1 |
| 8685 | CDS | 230797 | 231066 | 230797 | ID=cds.MS.gene034817.t1;Parent=MS.gene034817.t1 |
| Gene Sequence |
| Protein sequence |
