AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035065


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035065.t1 MTR_7g033800 97.093 172 5 0 1 172 1 172 2.20e-126 352
MS.gene035065.t1 MTR_6g005070 89.091 110 12 0 4 113 2 111 3.02e-69 207
MS.gene035065.t1 MTR_3g077240 86.087 115 15 1 1 115 1 114 5.29e-69 207
MS.gene035065.t1 MTR_6g005080 85.714 112 16 0 4 115 3 114 6.41e-68 203
MS.gene035065.t1 MTR_4g105170 63.953 172 47 3 1 157 1 172 1.77e-67 204
MS.gene035065.t1 MTR_8g040900 71.560 109 31 0 6 114 2 110 4.69e-50 160
MS.gene035065.t1 MTR_5g017950 66.364 110 37 0 5 114 2 111 1.79e-49 162
MS.gene035065.t1 MTR_5g083230 62.393 117 40 1 9 125 4 116 2.39e-49 157
MS.gene035065.t1 MTR_3g073690 66.667 111 37 0 5 115 3 113 1.87e-48 156
MS.gene035065.t1 MTR_5g083010 50.000 150 69 1 8 157 8 151 1.52e-47 153
MS.gene035065.t1 MTR_4g083680 64.078 103 37 0 10 112 14 116 5.59e-47 151
MS.gene035065.t1 MTR_2g068760 63.107 103 38 0 10 112 12 114 1.10e-46 150
MS.gene035065.t1 MTR_3g452660 67.290 107 35 0 8 114 4 110 1.63e-46 154
MS.gene035065.t1 MTR_3g071590 59.829 117 43 1 8 124 5 117 9.63e-46 147
MS.gene035065.t1 MTR_3g071420 67.010 97 32 0 8 104 11 107 4.65e-45 147
MS.gene035065.t1 MTR_3g031660 60.577 104 41 0 9 112 5 108 1.49e-44 145
MS.gene035065.t1 MTR_5g083960 59.821 112 45 0 3 114 31 142 2.08e-44 145
MS.gene035065.t1 MTR_3g094690 61.905 105 40 0 10 114 46 150 3.65e-44 146
MS.gene035065.t1 MTR_2g093310 59.223 103 42 0 10 112 32 134 1.05e-41 139
MS.gene035065.t1 MTR_3g094690 60.952 105 40 1 10 114 46 149 1.32e-41 139
MS.gene035065.t1 MTR_7g096530 60.000 115 42 2 1 111 1 115 1.44e-40 136
MS.gene035065.t1 MTR_4g060950 54.464 112 50 1 2 112 15 126 1.84e-40 135
MS.gene035065.t1 MTR_5g075020 63.810 105 38 0 10 114 42 146 1.69e-39 134
MS.gene035065.t1 MTR_5g075020 63.810 105 38 0 10 114 46 150 2.26e-39 134
MS.gene035065.t1 MTR_8g036085 59.048 105 42 1 11 114 23 127 7.11e-39 132
MS.gene035065.t1 MTR_1g095850 57.426 101 43 0 12 112 73 173 1.68e-38 131
MS.gene035065.t1 MTR_1g070220 57.265 117 45 2 1 112 1 117 3.72e-38 130
MS.gene035065.t1 MTR_8g036105 59.804 102 40 1 11 111 18 119 9.24e-36 124
MS.gene035065.t1 MTR_7g074990 60.417 96 37 1 11 105 20 115 2.21e-35 124
MS.gene035065.t1 MTR_7g028905 50.980 102 50 0 10 111 4 105 1.02e-34 118
MS.gene035065.t1 MTR_5g080470 55.670 97 42 1 10 105 6 102 9.51e-34 118
MS.gene035065.t1 MTR_8g079660 50.000 104 52 0 11 114 8 111 3.56e-33 119
MS.gene035065.t1 MTR_6g027710 51.923 104 49 1 10 112 39 142 5.55e-33 117
MS.gene035065.t1 MTR_7g096610 57.143 98 41 1 8 104 5 102 9.61e-33 116
MS.gene035065.t1 MTR_5g093010 46.552 116 58 1 12 127 6 117 2.58e-32 113
MS.gene035065.t1 MTR_7g075230 53.043 115 51 2 11 122 12 126 2.62e-32 114
MS.gene035065.t1 MTR_1g070205 54.737 95 42 1 11 104 12 106 5.57e-32 115
MS.gene035065.t1 MTR_8g079620 46.154 104 56 0 11 114 8 111 2.23e-29 108
MS.gene035065.t1 MTR_4g107450 36.306 157 88 4 12 161 11 162 1.85e-28 107
MS.gene035065.t1 MTR_6g011200 44.898 98 53 1 9 105 7 104 3.56e-28 105
MS.gene035065.t1 MTR_6g027700 48.515 101 51 1 11 110 7 107 4.61e-28 104
MS.gene035065.t1 MTR_6g011230 43.000 100 57 0 8 107 8 107 6.59e-27 100
MS.gene035065.t1 MTR_8g079580 46.667 105 55 1 11 114 8 112 1.15e-26 100
MS.gene035065.t1 MTR_6g011250 44.762 105 57 1 8 111 6 110 3.06e-26 99.0
MS.gene035065.t1 MTR_8g017090 40.385 104 62 0 8 111 9 112 2.24e-24 96.7
MS.gene035065.t1 MTR_4g099230 38.462 104 62 2 12 114 3 105 5.10e-23 89.7
MS.gene035065.t1 MTR_2g100020 41.667 96 56 0 12 107 20 115 1.23e-21 89.0
MS.gene035065.t1 MTR_5g085390 39.423 104 62 1 9 111 7 110 3.64e-20 84.3
MS.gene035065.t1 MTR_4g088035 35.556 90 58 0 10 99 5 94 1.86e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035065.t1 AT3G27650 84.071 113 18 0 5 117 33 145 9.44e-69 206
MS.gene035065.t1 AT5G63090 83.051 118 20 0 1 118 1 118 2.19e-67 203
MS.gene035065.t1 AT5G63090 83.051 118 20 0 1 118 1 118 2.19e-67 203
MS.gene035065.t1 AT5G63090 83.051 118 20 0 1 118 1 118 2.19e-67 203
MS.gene035065.t1 AT5G63090 83.051 118 20 0 1 118 1 118 2.19e-67 203
MS.gene035065.t1 AT1G65620 68.966 116 34 1 1 116 1 114 7.30e-50 159
MS.gene035065.t1 AT1G65620 68.966 116 34 1 1 116 1 114 7.30e-50 159
MS.gene035065.t1 AT1G65620 68.966 116 34 1 1 116 1 114 7.30e-50 159
MS.gene035065.t1 AT1G65620 68.966 116 34 1 1 116 1 114 7.30e-50 159
MS.gene035065.t1 AT1G65620 68.966 116 34 1 1 116 1 114 7.30e-50 159
MS.gene035065.t1 AT5G66870 65.455 110 38 0 6 115 2 111 1.46e-49 162
MS.gene035065.t1 AT2G30130 62.281 114 39 1 9 122 6 115 7.26e-48 154
MS.gene035065.t1 AT2G23660 59.813 107 43 0 8 114 2 108 2.75e-44 149
MS.gene035065.t1 AT2G23660 59.813 107 43 0 8 114 2 108 2.75e-44 149
MS.gene035065.t1 AT2G23660 59.813 107 43 0 8 114 2 108 2.75e-44 149
MS.gene035065.t1 AT1G16530 61.607 112 42 1 10 120 13 124 3.11e-44 144
MS.gene035065.t1 AT1G31320 59.322 118 46 1 10 125 12 129 3.87e-44 144
MS.gene035065.t1 AT2G30340 55.932 118 47 1 10 122 52 169 2.84e-43 145
MS.gene035065.t1 AT2G30340 55.932 118 47 1 10 122 51 168 3.15e-43 144
MS.gene035065.t1 AT3G11090 59.259 108 44 0 8 115 8 115 7.07e-43 140
MS.gene035065.t1 AT1G07900 59.223 103 42 0 10 112 32 134 9.65e-42 138
MS.gene035065.t1 AT2G28500 59.223 103 42 0 10 112 54 156 3.05e-41 138
MS.gene035065.t1 AT2G40470 61.905 105 40 0 10 114 19 123 5.38e-40 134
MS.gene035065.t1 AT2G40470 61.905 105 40 0 10 114 44 148 1.65e-39 134
MS.gene035065.t1 AT3G26660 51.304 115 55 1 12 125 6 120 1.94e-39 130
MS.gene035065.t1 AT3G26620 56.000 100 44 0 12 111 6 105 3.93e-39 129
MS.gene035065.t1 AT2G42430 55.372 121 49 2 1 116 1 121 9.01e-38 130
MS.gene035065.t1 AT3G03760 57.692 104 43 1 10 112 50 153 2.27e-37 130
MS.gene035065.t1 AT4G00210 55.357 112 49 1 1 111 1 112 2.08e-36 125
MS.gene035065.t1 AT4G00220 60.606 99 38 1 14 111 20 118 2.43e-36 125
MS.gene035065.t1 AT2G45420 60.606 99 38 1 14 111 40 138 4.31e-36 126
MS.gene035065.t1 AT5G06080 51.402 107 51 1 10 115 6 112 4.16e-34 119
MS.gene035065.t1 AT2G45410 44.937 158 79 4 14 170 19 169 1.49e-33 117
MS.gene035065.t1 AT2G31310 55.208 96 42 1 10 104 6 101 5.74e-33 116
MS.gene035065.t1 AT3G58190 55.660 106 46 1 1 105 1 106 8.57e-33 116
MS.gene035065.t1 AT2G42440 52.525 99 46 1 8 105 4 102 1.36e-32 117
MS.gene035065.t1 AT3G50510 47.170 106 56 0 9 114 10 115 7.63e-30 108
MS.gene035065.t1 AT3G50510 47.170 106 56 0 9 114 10 115 7.63e-30 108
MS.gene035065.t1 AT5G35900 41.071 112 66 0 12 123 6 117 9.01e-27 100
MS.gene035065.t1 AT3G13850 35.433 127 82 0 12 138 37 163 4.95e-26 100
MS.gene035065.t1 AT3G47870 37.615 109 68 0 8 116 33 141 1.08e-25 100
MS.gene035065.t1 AT1G72980 40.541 111 66 0 1 111 3 113 4.31e-24 94.0
MS.gene035065.t1 AT1G06280 43.011 93 52 1 12 104 25 116 3.59e-20 83.6
MS.gene035065.t1 AT4G22700 36.937 111 68 2 12 121 6 115 8.02e-18 77.0
MS.gene035065.t1 AT2G19820 37.363 91 56 1 8 97 9 99 6.84e-16 70.5
MS.gene035065.t1 AT5G15060 43.243 74 38 2 12 82 18 90 1.70e-14 67.0

Find 43 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATGTTCACAAAATATT+TGG 0.149406 7.4:-67884643 MS.gene035065:CDS
GCAAATTTCTGTGGCTCTTC+TGG 0.194109 7.4:+67884664 None:intergenic
AGGATTAAAGATCCAGTTTA+TGG 0.225345 7.4:-67884532 MS.gene035065:CDS
GGCTCTTCTGGTGGGAAATA+TGG 0.297872 7.4:+67884676 None:intergenic
AATCCTTGCCTCGGCTTCAT+AGG 0.305194 7.4:+67884549 None:intergenic
TAGTAGAATCAAGTTCCTTT+TGG 0.319682 7.4:+67884464 None:intergenic
GGAGCCTAAGAACTTGTCTT+TGG 0.328487 7.4:+67884485 None:intergenic
ATCATGATATCGCAGCTATA+AGG 0.383935 7.4:-67884399 MS.gene035065:CDS
GCACCAACACAACCATAAAC+TGG 0.396732 7.4:+67884520 None:intergenic
TCCTCCAAAGACAAGTTCTT+AGG 0.431787 7.4:-67884489 MS.gene035065:CDS
ATAAGGATCGGTGGGGAAGA+TGG 0.446744 7.4:-67884382 MS.gene035065:CDS
TTGTCTTTGGAGGACTGAAA+TGG 0.450349 7.4:+67884498 None:intergenic
TATGGAGCAAATATGCAATC+TGG 0.454516 7.4:+67884694 None:intergenic
AATTTCTGTGGCTCTTCTGG+TGG 0.462397 7.4:+67884667 None:intergenic
GCCTAAGAACTTGTCTTTGG+AGG 0.492984 7.4:+67884488 None:intergenic
TCTCGGTCAATCTCCTCCTC+CGG 0.512178 7.4:-67884347 MS.gene035065:CDS
TGGATCTTTAATCCTTGCCT+CGG 0.516855 7.4:+67884540 None:intergenic
ACAAGTTCTTAGGCTCCAAA+AGG 0.517159 7.4:-67884479 MS.gene035065:CDS
GATGGAGGTGGTTCTTCTCT+CGG 0.519166 7.4:-67884364 MS.gene035065:CDS
TCATTATTGTTCCAAGGAGA+AGG 0.524865 7.4:+67884313 None:intergenic
ATAGCTGCGATATCATGATT+CGG 0.525283 7.4:+67884403 None:intergenic
TGAAGTGCAACAACATCAAA+GGG 0.528377 7.4:-67884593 MS.gene035065:CDS
GATGGTTATCACACGGGAGG+AGG 0.542341 7.4:-67884280 MS.gene035065:CDS
GATCCAGTTTATGGTTGTGT+TGG 0.554848 7.4:-67884523 MS.gene035065:CDS
TCGCAGCTATAAGGATCGGT+GGG 0.558420 7.4:-67884390 MS.gene035065:CDS
ATGAAGTGCAACAACATCAA+AGG 0.561125 7.4:-67884594 MS.gene035065:CDS
ATTTCTGTGGCTCTTCTGGT+GGG 0.562695 7.4:+67884668 None:intergenic
CTCTCTTGCCTATGAAGCCG+AGG 0.568060 7.4:-67884557 MS.gene035065:CDS
AGGATCGGTGGGGAAGATGG+AGG 0.572677 7.4:-67884379 MS.gene035065:CDS
AGTGGATCATTATTGTTCCA+AGG 0.579950 7.4:+67884307 None:intergenic
TGAACATTTGCAAATTTCTG+TGG 0.582165 7.4:+67884655 None:intergenic
ATCGGTGGGGAAGATGGAGG+TGG 0.584704 7.4:-67884376 MS.gene035065:CDS
ATCGCAGCTATAAGGATCGG+TGG 0.589681 7.4:-67884391 MS.gene035065:CDS
TTGCCTATGAAGCCGAGGCA+AGG 0.591021 7.4:-67884552 MS.gene035065:CDS
AATAATGATCCACTTGGCGA+TGG 0.592711 7.4:-67884298 MS.gene035065:CDS
TGGAACAATAATGATCCACT+TGG 0.604864 7.4:-67884304 MS.gene035065:CDS
AGCACATGGTGAATTTGAGT+AGG 0.632061 7.4:+67884747 None:intergenic
GATATCGCAGCTATAAGGAT+CGG 0.642191 7.4:-67884394 MS.gene035065:CDS
CTTGGCGATGGTTATCACAC+GGG 0.658586 7.4:-67884286 MS.gene035065:CDS
ACTTGGCGATGGTTATCACA+CGG 0.661040 7.4:-67884287 MS.gene035065:CDS
GGCGATGGTTATCACACGGG+AGG 0.663949 7.4:-67884283 MS.gene035065:CDS
GTGTGATAACCATCGCCAAG+TGG 0.665247 7.4:+67884289 None:intergenic
CGCAGCTATAAGGATCGGTG+GGG 0.674584 7.4:-67884389 MS.gene035065:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GCAAATGTTCACAAAATATT+TGG - chr7.4:67884387-67884406 MS.gene035065:CDS 25.0%
AGGATTAAAGATCCAGTTTA+TGG - chr7.4:67884498-67884517 MS.gene035065:CDS 30.0%
TGAACATTTGCAAATTTCTG+TGG + chr7.4:67884378-67884397 None:intergenic 30.0%
! TAGTAGAATCAAGTTCCTTT+TGG + chr7.4:67884569-67884588 None:intergenic 30.0%
AGTGGATCATTATTGTTCCA+AGG + chr7.4:67884726-67884745 None:intergenic 35.0%
ATAGCTGCGATATCATGATT+CGG + chr7.4:67884630-67884649 None:intergenic 35.0%
ATCATGATATCGCAGCTATA+AGG - chr7.4:67884631-67884650 MS.gene035065:CDS 35.0%
ATGAAGTGCAACAACATCAA+AGG - chr7.4:67884436-67884455 MS.gene035065:CDS 35.0%
TATGGAGCAAATATGCAATC+TGG + chr7.4:67884339-67884358 None:intergenic 35.0%
TCATTATTGTTCCAAGGAGA+AGG + chr7.4:67884720-67884739 None:intergenic 35.0%
TGAAGTGCAACAACATCAAA+GGG - chr7.4:67884437-67884456 MS.gene035065:CDS 35.0%
TGGAACAATAATGATCCACT+TGG - chr7.4:67884726-67884745 MS.gene035065:CDS 35.0%
! CTTTTTACTATCCTTCTCCT+TGG - chr7.4:67884706-67884725 MS.gene035065:CDS 35.0%
AATAATGATCCACTTGGCGA+TGG - chr7.4:67884732-67884751 MS.gene035065:CDS 40.0%
ACAAGTTCTTAGGCTCCAAA+AGG - chr7.4:67884551-67884570 MS.gene035065:CDS 40.0%
AGCACATGGTGAATTTGAGT+AGG + chr7.4:67884286-67884305 None:intergenic 40.0%
GATATCGCAGCTATAAGGAT+CGG - chr7.4:67884636-67884655 MS.gene035065:CDS 40.0%
GATCCAGTTTATGGTTGTGT+TGG - chr7.4:67884507-67884526 MS.gene035065:CDS 40.0%
GGAGAAGGATAGTAAAAAGC+CGG + chr7.4:67884705-67884724 None:intergenic 40.0%
TCCTCCAAAGACAAGTTCTT+AGG - chr7.4:67884541-67884560 MS.gene035065:CDS 40.0%
TGGATCTTTAATCCTTGCCT+CGG + chr7.4:67884493-67884512 None:intergenic 40.0%
! TTGTCTTTGGAGGACTGAAA+TGG + chr7.4:67884535-67884554 None:intergenic 40.0%
AAAAACTTGCACGCAGCACA+TGG + chr7.4:67884300-67884319 None:intergenic 45.0%
AATTTCTGTGGCTCTTCTGG+TGG + chr7.4:67884366-67884385 None:intergenic 45.0%
ACTTGGCGATGGTTATCACA+CGG - chr7.4:67884743-67884762 MS.gene035065:CDS 45.0%
ATTTCTGTGGCTCTTCTGGT+GGG + chr7.4:67884365-67884384 None:intergenic 45.0%
GAAGGATAGTAAAAAGCCGG+AGG + chr7.4:67884702-67884721 None:intergenic 45.0%
GCAAATTTCTGTGGCTCTTC+TGG + chr7.4:67884369-67884388 None:intergenic 45.0%
GCACCAACACAACCATAAAC+TGG + chr7.4:67884513-67884532 None:intergenic 45.0%
GCCTAAGAACTTGTCTTTGG+AGG + chr7.4:67884545-67884564 None:intergenic 45.0%
GGAGCCTAAGAACTTGTCTT+TGG + chr7.4:67884548-67884567 None:intergenic 45.0%
!! CTGCGTGCAAGTTTTTGAGA+AGG - chr7.4:67884304-67884323 MS.gene035065:CDS 45.0%
AATCCTTGCCTCGGCTTCAT+AGG + chr7.4:67884484-67884503 None:intergenic 50.0%
ATAAGGATCGGTGGGGAAGA+TGG - chr7.4:67884648-67884667 MS.gene035065:CDS 50.0%
ATCGCAGCTATAAGGATCGG+TGG - chr7.4:67884639-67884658 MS.gene035065:CDS 50.0%
CTTGGCGATGGTTATCACAC+GGG - chr7.4:67884744-67884763 MS.gene035065:CDS 50.0%
GATGGAGGTGGTTCTTCTCT+CGG - chr7.4:67884666-67884685 MS.gene035065:CDS 50.0%
GGATAGTAAAAAGCCGGAGG+AGG + chr7.4:67884699-67884718 None:intergenic 50.0%
GGCTCTTCTGGTGGGAAATA+TGG + chr7.4:67884357-67884376 None:intergenic 50.0%
GTGTGATAACCATCGCCAAG+TGG + chr7.4:67884744-67884763 None:intergenic 50.0%
TCGCAGCTATAAGGATCGGT+GGG - chr7.4:67884640-67884659 MS.gene035065:CDS 50.0%
CGCAGCTATAAGGATCGGTG+GGG - chr7.4:67884641-67884660 MS.gene035065:CDS 55.0%
CTCTCTTGCCTATGAAGCCG+AGG - chr7.4:67884473-67884492 MS.gene035065:CDS 55.0%
GATGGTTATCACACGGGAGG+AGG - chr7.4:67884750-67884769 MS.gene035065:CDS 55.0%
TCTCGGTCAATCTCCTCCTC+CGG - chr7.4:67884683-67884702 MS.gene035065:CDS 55.0%
TTGCCTATGAAGCCGAGGCA+AGG - chr7.4:67884478-67884497 MS.gene035065:CDS 55.0%
AGGATCGGTGGGGAAGATGG+AGG - chr7.4:67884651-67884670 MS.gene035065:CDS 60.0%
ATCGGTGGGGAAGATGGAGG+TGG - chr7.4:67884654-67884673 MS.gene035065:CDS 60.0%
GGCGATGGTTATCACACGGG+AGG - chr7.4:67884747-67884766 MS.gene035065:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 67884267 67884785 67884267 ID=MS.gene035065
chr7.4 mRNA 67884267 67884785 67884267 ID=MS.gene035065.t1;Parent=MS.gene035065
chr7.4 exon 67884267 67884785 67884267 ID=MS.gene035065.t1.exon1;Parent=MS.gene035065.t1
chr7.4 CDS 67884267 67884785 67884267 ID=cds.MS.gene035065.t1;Parent=MS.gene035065.t1
Gene Sequence

>MS.gene035065

ATGGCATCTTCAAGCGCCTACTCAAATTCACCATGTGCTGCGTGCAAGTTTTTGAGAAGGAAGTGCATGCCAGATTGCATATTTGCTCCATATTTCCCACCAGAAGAGCCACAGAAATTTGCAAATGTTCACAAAATATTTGGTGCAAGCAATGTGAGTAAACTTCTGAATGAAGTGCAACAACATCAAAGGGAAGATGCAGTGAACTCTCTTGCCTATGAAGCCGAGGCAAGGATTAAAGATCCAGTTTATGGTTGTGTTGGTGCCATTTCAGTCCTCCAAAGACAAGTTCTTAGGCTCCAAAAGGAACTTGATTCTACTAACGCTGATTTGATTCGCTATACTACTTGCACTGAAATTCCGAATCATGATATCGCAGCTATAAGGATCGGTGGGGAAGATGGAGGTGGTTCTTCTCTCGGTCAATCTCCTCCTCCGGCTTTTTACTATCCTTCTCCTTGGAACAATAATGATCCACTTGGCGATGGTTATCACACGGGAGGAGGTGACAATATATAA

Protein sequence

>MS.gene035065.t1

MASSSAYSNSPCAACKFLRRKCMPDCIFAPYFPPEEPQKFANVHKIFGASNVSKLLNEVQQHQREDAVNSLAYEAEARIKDPVYGCVGAISVLQRQVLRLQKELDSTNADLIRYTTCTEIPNHDIAAIRIGGEDGGGSSLGQSPPPAFYYPSPWNNNDPLGDGYHTGGGDNI