AlfalfaGEDB | MS.gene035159

M. sativa cultivar XinJiangDaYe / MS.gene035159

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035159.t1	XP_003614286.1	95.5	156	7	0	1	156	1	156	1.40E-74	288.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035159.t1	Q9FJK3	47.4	156	81	1	1	155	1	156	3.7e-31	136.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035159.t1	G7JY85	95.5	156	7	0	1	156	1	156	1.0e-74	288.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene035159.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035159.t1	MTR_5g047580	95.513	156	7	0	1	156	1	156	2.64e-108	305
MS.gene035159.t1	MTR_1g084950	85.897	156	22	0	1	156	1	156	9.98e-88	253
MS.gene035159.t1	MTR_5g047560	81.410	156	13	1	1	156	1	140	8.27e-87	250
MS.gene035159.t1	MTR_7g011950	75.000	156	39	0	1	156	1	156	1.05e-75	223
MS.gene035159.t1	MTR_4g028720	75.000	156	39	0	1	156	1	156	4.86e-75	221
MS.gene035159.t1	MTR_1g077390	68.590	156	49	0	1	156	1	156	1.36e-74	220
MS.gene035159.t1	MTR_1g077320	72.436	156	43	0	1	156	1	156	3.02e-73	216
MS.gene035159.t1	MTR_1g077300	71.154	156	43	1	1	156	1	154	2.17e-67	202
MS.gene035159.t1	MTR_1g090783	64.052	153	55	0	3	155	2	154	1.37e-65	197
MS.gene035159.t1	MTR_1g090697	58.710	155	64	0	1	155	1	155	1.92e-61	186
MS.gene035159.t1	MTR_1g090710	56.410	156	67	1	1	155	1	156	7.06e-59	180
MS.gene035159.t1	MTR_3g065100	51.282	156	75	1	1	155	1	156	2.16e-42	141
MS.gene035159.t1	MTR_3g466980	44.805	154	84	1	1	153	1	154	9.47e-40	134
MS.gene035159.t1	MTR_4g032620	40.909	154	89	2	1	152	1	154	2.97e-39	132
MS.gene035159.t1	MTR_4g063790	42.857	154	86	2	1	152	1	154	4.55e-36	122
MS.gene035159.t1	MTR_3g466830	42.857	154	87	1	3	155	2	155	6.72e-34	117
MS.gene035159.t1	MTR_8g036130	42.767	159	87	2	1	155	1	159	2.02e-33	118
MS.gene035159.t1	MTR_5g075380	35.897	156	99	1	1	155	1	156	1.70e-32	113
MS.gene035159.t1	MTR_4g031910	43.590	156	86	2	1	154	1	156	2.81e-32	115
MS.gene035159.t1	MTR_3g031240	37.580	157	96	2	1	155	1	157	8.50e-31	109
MS.gene035159.t1	MTR_3g467080	38.217	157	95	2	1	155	1	157	1.84e-30	108
MS.gene035159.t1	MTR_3g466890	39.610	154	92	1	3	155	2	155	7.45e-30	106
MS.gene035159.t1	MTR_4g032260	42.949	156	87	2	1	154	1	156	8.74e-29	103
MS.gene035159.t1	MTR_3g031100	37.342	158	95	3	1	155	1	157	1.09e-28	106
MS.gene035159.t1	MTR_2g035610	45.763	118	63	1	1	117	1	118	1.10e-28	102
MS.gene035159.t1	MTR_4g032290	44.231	156	85	2	1	154	1	156	4.35e-28	104
MS.gene035159.t1	MTR_2g016210	37.086	151	93	2	1	149	1	151	2.49e-26	100
MS.gene035159.t1	MTR_4g028800	53.425	73	32	1	1	73	1	71	3.04e-23	87.4
MS.gene035159.t1	MTR_2g035580	34.194	155	101	1	1	154	1	155	4.62e-23	89.4
MS.gene035159.t1	MTR_3g465410	38.889	126	75	2	29	152	1	126	5.24e-23	88.6
MS.gene035159.t1	MTR_4g019670	34.899	149	85	4	1	145	1	141	1.23e-18	82.0
MS.gene035159.t1	MTR_7g106510	32.639	144	88	3	3	145	6	141	9.28e-18	79.3
MS.gene035159.t1	MTR_3g466900	34.400	125	81	1	32	155	2	126	2.47e-17	73.9
MS.gene035159.t1	MTR_3g466930	34.400	125	81	1	32	155	2	126	2.93e-17	73.6
MS.gene035159.t1	MTR_1g033930	45.161	93	50	1	64	155	22	114	6.81e-17	72.4
MS.gene035159.t1	MTR_7g055800	35.043	117	49	2	1	117	1	90	2.26e-16	70.1
MS.gene035159.t1	MTR_7g055790	39.640	111	48	2	1	110	59	151	6.48e-16	70.5
MS.gene035159.t1	MTR_1g033370	43.000	100	56	1	57	155	21	120	6.66e-15	67.4
MS.gene035159.t1	MTR_1g033790	42.222	90	51	1	64	152	22	111	2.44e-13	63.2
MS.gene035159.t1	MTR_6g005450	31.481	108	68	3	1	102	1	108	1.88e-12	63.9
MS.gene035159.t1	MTR_7g055940	34.314	102	40	2	1	102	1	75	2.75e-12	59.3
MS.gene035159.t1	MTR_6g018920	26.875	160	97	6	1	154	1	146	1.96e-11	60.8
MS.gene035159.t1	MTR_3g093900	28.829	111	78	1	3	112	6	116	2.29e-11	61.2
MS.gene035159.t1	MTR_1g114730	28.440	109	70	3	1	102	1	108	2.87e-11	60.5
MS.gene035159.t1	MTR_5g066180	34.513	113	57	3	1	113	1	96	7.11e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035159.t1	AT5G26630	46.154	156	83	1	1	155	1	156	5.52e-39	132
MS.gene035159.t1	AT5G48670	47.436	156	81	1	1	155	1	156	1.41e-36	128
MS.gene035159.t1	AT1G65330	42.857	154	87	1	3	155	2	155	2.78e-36	126
MS.gene035159.t1	AT1G65300	40.909	154	90	1	3	155	2	155	1.04e-34	122
MS.gene035159.t1	AT3G05860	41.611	149	86	1	1	148	1	149	1.79e-34	120
MS.gene035159.t1	AT3G05860	41.611	149	86	1	1	148	1	149	6.53e-34	119
MS.gene035159.t1	AT3G05860	41.611	149	86	1	1	148	1	149	1.14e-33	119
MS.gene035159.t1	AT5G26650	37.908	153	94	1	4	155	2	154	3.88e-30	112
MS.gene035159.t1	AT5G27960	36.601	153	96	1	4	155	2	154	4.42e-28	106
MS.gene035159.t1	AT1G31630	31.126	151	102	2	3	152	2	151	3.62e-25	99.0
MS.gene035159.t1	AT1G22590	33.333	156	100	3	1	155	1	153	8.89e-23	89.0
MS.gene035159.t1	AT1G31640	34.000	150	97	2	3	151	2	150	1.19e-22	93.2
MS.gene035159.t1	AT2G28700	38.017	121	68	2	1	117	1	118	3.04e-21	88.2
MS.gene035159.t1	AT5G27810	43.333	90	50	1	29	117	1	90	6.05e-21	82.8
MS.gene035159.t1	AT5G26580	34.194	155	89	1	1	155	1	142	1.75e-20	86.3
MS.gene035159.t1	AT2G40210	36.154	130	78	2	1	127	1	128	4.05e-16	74.3
MS.gene035159.t1	AT5G06500	34.247	146	83	5	1	141	1	138	2.69e-15	70.9
MS.gene035159.t1	AT5G58890	35.417	96	55	2	1	89	1	96	1.66e-11	60.8
MS.gene035159.t1	AT1G47760	33.333	111	67	3	1	110	1	105	2.38e-11	59.3
MS.gene035159.t1	AT5G55690	29.167	120	78	2	1	113	1	120	4.45e-11	59.7
MS.gene035159.t1	AT5G55690	29.167	120	78	2	1	113	1	120	4.45e-11	59.7

Find 25 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATCTTTCATTGATGAGTTC+TGG	0.252705	5.2:+37965719	None:intergenic
CTCTAATGATTCGGCAAGAA+AGG	0.394804	5.2:-37965899	MS.gene035159:CDS
GAGCTGAACAATCTTATGAT+TGG	0.404054	5.2:-37965601	MS.gene035159:CDS
CTTTAGTAATCCTCTGCAAT+AGG	0.413033	5.2:+37965668	None:intergenic
GTACCTCTCAATTACCTGTC+TGG	0.443679	5.2:+37965741	None:intergenic
TGAGAAACAGATAATTCATC+TGG	0.457651	5.2:+37965556	None:intergenic
AGCTGAACAATCTTATGATT+GGG	0.458403	5.2:-37965600	MS.gene035159:CDS
AGAGAACTCACCATTCTTTG+TGG	0.496533	5.2:-37965835	MS.gene035159:CDS
GCTCCTTCTCACGATTATCA+TGG	0.497017	5.2:+37965620	None:intergenic
TGCTGGAATACCACAAAGAA+TGG	0.514499	5.2:+37965825	None:intergenic
TGAGAAAATTCTGAAGGACA+TGG	0.544670	5.2:-37965509	MS.gene035159:CDS
TTGGAAATTATAGCACATGC+TGG	0.559487	5.2:+37965808	None:intergenic
TCTGCAATAGGAAACTCTCT+TGG	0.562811	5.2:+37965680	None:intergenic
AGAGGTGTGGCCAGATCTAG+AGG	0.572723	5.2:-37965767	MS.gene035159:CDS
CAACGCCATGACTAGGAAGA+AGG	0.575303	5.2:-37965938	None:intergenic
GCTTATTGAGAAAATTCTGA+AGG	0.584834	5.2:-37965515	MS.gene035159:CDS
AAGAGAGTTTCCTATTGCAG+AGG	0.589804	5.2:-37965678	MS.gene035159:CDS
TTAATTCTAAAACAGAGGTG+TGG	0.602716	5.2:-37965780	MS.gene035159:CDS
TGCATCACAACGCCATGACT+AGG	0.604632	5.2:-37965945	None:intergenic
GATAACAAGATCGATGCACT+TGG	0.609178	5.2:-37965487	MS.gene035159:CDS
GAGGTGTGGCCAGATCTAGA+GGG	0.615970	5.2:-37965766	MS.gene035159:CDS
AAGCCATGATAATCGTGAGA+AGG	0.628086	5.2:-37965623	MS.gene035159:CDS
AGGTGTGGCCAGATCTAGAG+GGG	0.656006	5.2:-37965765	MS.gene035159:CDS
GGGCCAGACAGGTAATTGAG+AGG	0.686598	5.2:-37965744	MS.gene035159:CDS
GGTGTGGCCAGATCTAGAGG+GGG	0.723497	5.2:-37965764	MS.gene035159:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACCTACAATAAAAGAAAAA+AGG	-	chr5.2:37965539-37965558	MS.gene035159:CDS	20.0%
!!	ACCTACAATAAAAGAAAAAA+GGG	-	chr5.2:37965540-37965559	MS.gene035159:CDS	20.0%
!!!	TGTTTTAGAATTAAAAGGAT+TGG	+	chr5.2:37965628-37965647	None:intergenic	20.0%
!!	TGCTTTTATCTCTAATGATT+CGG	-	chr5.2:37965506-37965525	MS.gene035159:CDS	25.0%
!!!	ACCCTTTTTTCTTTTATTGT+AGG	+	chr5.2:37965544-37965563	None:intergenic	25.0%
!!!	ACCTCTGTTTTAGAATTAAA+AGG	+	chr5.2:37965633-37965652	None:intergenic	25.0%
!!!	TCCTTTTAATTCTAAAACAG+AGG	-	chr5.2:37965629-37965648	MS.gene035159:CDS	25.0%
	AGCTGAACAATCTTATGATT+GGG	-	chr5.2:37965814-37965833	MS.gene035159:CDS	30.0%
	GCTTATTGAGAAAATTCTGA+AGG	-	chr5.2:37965899-37965918	MS.gene035159:CDS	30.0%
	TGAGAAACAGATAATTCATC+TGG	+	chr5.2:37965861-37965880	None:intergenic	30.0%
!!	AAAAAAGGGTATCATCAAGA+AGG	-	chr5.2:37965554-37965573	MS.gene035159:CDS	30.0%
!!	TTAATTCTAAAACAGAGGTG+TGG	-	chr5.2:37965634-37965653	MS.gene035159:CDS	30.0%
	CATCTTTCATTGATGAGTTC+TGG	+	chr5.2:37965698-37965717	None:intergenic	35.0%
	CTTTAGTAATCCTCTGCAAT+AGG	+	chr5.2:37965749-37965768	None:intergenic	35.0%
	GAGCTGAACAATCTTATGAT+TGG	-	chr5.2:37965813-37965832	MS.gene035159:CDS	35.0%
	TTGGAAATTATAGCACATGC+TGG	+	chr5.2:37965609-37965628	None:intergenic	35.0%
!	TGAGAAAATTCTGAAGGACA+TGG	-	chr5.2:37965905-37965924	MS.gene035159:CDS	35.0%
	AAGAGAGTTTCCTATTGCAG+AGG	-	chr5.2:37965736-37965755	MS.gene035159:CDS	40.0%
	AAGCCATGATAATCGTGAGA+AGG	-	chr5.2:37965791-37965810	MS.gene035159:CDS	40.0%
	AGAGAACTCACCATTCTTTG+TGG	-	chr5.2:37965579-37965598	MS.gene035159:CDS	40.0%
	GATAACAAGATCGATGCACT+TGG	-	chr5.2:37965927-37965946	MS.gene035159:CDS	40.0%
	TCTGCAATAGGAAACTCTCT+TGG	+	chr5.2:37965737-37965756	None:intergenic	40.0%
	TGCTGGAATACCACAAAGAA+TGG	+	chr5.2:37965592-37965611	None:intergenic	40.0%
!	CTCTAATGATTCGGCAAGAA+AGG	-	chr5.2:37965515-37965534	MS.gene035159:CDS	40.0%
!!!	TTGCTTTTGAAGTTGCTCTC+TGG	+	chr5.2:37965772-37965791	None:intergenic	40.0%
	GCTCCTTCTCACGATTATCA+TGG	+	chr5.2:37965797-37965816	None:intergenic	45.0%
	GTACCTCTCAATTACCTGTC+TGG	+	chr5.2:37965676-37965695	None:intergenic	45.0%
	AGGTGTGGCCAGATCTAGAG+GGG	-	chr5.2:37965649-37965668	MS.gene035159:CDS	55.0%
	GAGGTGTGGCCAGATCTAGA+GGG	-	chr5.2:37965648-37965667	MS.gene035159:CDS	55.0%
	GGGCCAGACAGGTAATTGAG+AGG	-	chr5.2:37965670-37965689	MS.gene035159:CDS	55.0%
!	AGAGGTGTGGCCAGATCTAG+AGG	-	chr5.2:37965647-37965666	MS.gene035159:CDS	55.0%
	AGATCTAGAGGGGGCCAGAC+AGG	-	chr5.2:37965659-37965678	MS.gene035159:CDS	60.0%
	GGTGTGGCCAGATCTAGAGG+GGG	-	chr5.2:37965650-37965669	MS.gene035159:CDS	60.0%
	TGTCTGGCCCCCTCTAGATC+TGG	+	chr5.2:37965660-37965679	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	37965483	37965953	37965483	ID=MS.gene035159
chr5.2	mRNA	37965483	37965953	37965483	ID=MS.gene035159.t1;Parent=MS.gene035159
chr5.2	exon	37965483	37965953	37965483	ID=MS.gene035159.t1.exon1;Parent=MS.gene035159.t1
chr5.2	CDS	37965483	37965953	37965483	ID=cds.MS.gene035159.t1;Parent=MS.gene035159.t1

Gene Sequence

>MS.gene035159

ATGACTAGGAAGAAGGTAAAACTTGCTTTTATCTCTAATGATTCGGCAAGAAAGGCAACCTACAATAAAAGAAAAAAGGGTATCATCAAGAAGGTTAGAGAACTCACCATTCTTTGTGGTATTCCAGCATGTGCTATAATTTCCAATCCTTTTAATTCTAAAACAGAGGTGTGGCCAGATCTAGAGGGGGCCAGACAGGTAATTGAGAGGTACCAGAACTCATCAATGAAAGATGAAACAAAGAATGTGAACCAAGAGAGTTTCCTATTGCAGAGGATTACTAAAGCCAGAGAGCAACTTCAAAAGCAAAGCCATGATAATCGTGAGAAGGAGCTGAACAATCTTATGATTGGGTATATGAAAAATAAAAAGCTACCAGATGAATTATCTGTTTCTCAATTGAAAGACTTTGATAAGCTTATTGAGAAAATTCTGAAGGACATGGATAACAAGATCGATGCACTTGGTTGA

Protein sequence

>MS.gene035159.t1

MTRKKVKLAFISNDSARKATYNKRKKGIIKKVRELTILCGIPACAIISNPFNSKTEVWPDLEGARQVIERYQNSSMKDETKNVNQESFLLQRITKAREQLQKQSHDNREKELNNLMIGYMKNKKLPDELSVSQLKDFDKLIEKILKDMDNKIDALG