AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene03518

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03518.t1 MTR_8g019520 79.012 81 17 0 190 270 176 256 9.83e-32 118
MS.gene03518.t1 MTR_8g019520 79.012 81 17 0 190 270 177 257 1.03e-31 118
MS.gene03518.t1 MTR_2g099500 75.862 58 14 0 212 269 193 250 8.86e-27 105
MS.gene03518.t1 MTR_1g015185 76.364 55 12 1 216 270 210 263 2.46e-24 98.6
MS.gene03518.t1 MTR_2g040990 68.333 60 16 2 213 270 195 253 3.30e-22 92.8
MS.gene03518.t1 MTR_4g134510 69.231 52 16 0 215 266 201 252 1.31e-21 91.3
MS.gene03518.t1 MTR_4g015830 69.091 55 16 1 216 270 187 240 8.20e-21 89.0
MS.gene03518.t1 MTR_7g068590 65.455 55 18 1 216 270 189 242 2.14e-19 85.1
MS.gene03518.t1 MTR_0024s0330 52.273 88 38 2 1 88 96 179 7.55e-17 80.5
MS.gene03518.t1 MTR_6g017265 51.250 80 35 2 2 81 106 181 7.27e-14 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene03518.t1 AT5G20510 71.186 59 16 1 212 270 162 219 1.10e-24 98.6
MS.gene03518.t1 AT5G20510 71.186 59 16 1 212 270 203 260 1.90e-24 99.0
MS.gene03518.t1 AT3G42790 74.138 58 14 1 212 269 193 249 3.61e-24 98.2
MS.gene03518.t1 AT2G02470 74.545 55 13 1 216 270 203 256 7.88e-24 97.4
MS.gene03518.t1 AT2G02470 74.545 55 13 1 216 270 194 247 1.11e-23 96.7
MS.gene03518.t1 AT5G26210 75.000 56 13 1 214 269 200 254 3.19e-23 95.5
MS.gene03518.t1 AT1G14510 68.519 54 17 0 216 269 198 251 4.82e-22 92.4
MS.gene03518.t1 AT5G05610 63.636 55 20 0 212 266 184 238 1.87e-19 85.1
MS.gene03518.t1 AT5G05610 63.636 55 20 0 212 266 184 238 1.87e-19 85.1
MS.gene03518.t1 AT3G11200 62.745 51 19 0 212 262 189 239 5.82e-18 81.3
MS.gene03518.t1 AT3G11200 62.745 51 19 0 212 262 176 226 8.77e-18 80.5
MS.gene03518.t1 AT5G38690 26.512 215 141 3 1 198 82 296 6.64e-12 65.9
MS.gene03518.t1 AT5G38690 26.512 215 141 3 1 198 82 296 6.64e-12 65.9

Find 67 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAACTCATACAATAAAT+TGG 0.148698 6.2:-38048778 MS.gene03518:CDS
AAAGCATATTCTTCCTTTAT+AGG 0.158707 6.2:+38049080 None:intergenic
TGGGATGGCACCATTGTTAT+TGG 0.285100 6.2:-38048758 MS.gene03518:CDS
TCTTCCACATCCTCGTCTTT+AGG 0.289074 6.2:+38048426 None:intergenic
TACTAATGTTGGGCCTATAA+AGG 0.359306 6.2:-38049093 MS.gene03518:CDS
GTTATTGGTGATAATGATAT+TGG 0.371338 6.2:-38048743 MS.gene03518:CDS
GAAGTTGATCTATCTCCATA+TGG 0.371660 6.2:+38048708 None:intergenic
CAGAAAATCCAGAATTCATC+AGG 0.388348 6.2:+38048336 None:intergenic
GGCAATGGGTACCGAGGATT+GGG 0.397506 6.2:-38048837 MS.gene03518:CDS
TGCAAATGCCTCTACACTTC+GGG 0.407999 6.2:+38049356 None:intergenic
TAATTGATAATAATGCTATA+AGG 0.408256 6.2:-38048682 MS.gene03518:CDS
TATAAAGGAAGAATATGCTT+TGG 0.417141 6.2:-38049078 MS.gene03518:CDS
TCCTCAATTAGTTCTCTCTC+TGG 0.418949 6.2:+38048659 None:intergenic
TGGCAATGGGTACCGAGGAT+TGG 0.420412 6.2:-38048838 MS.gene03518:CDS
ATAAAGGAAGAATATGCTTT+GGG 0.425102 6.2:-38049077 MS.gene03518:CDS
ATGGGTAATAATACTAATGT+TGG 0.425773 6.2:-38049104 MS.gene03518:CDS
GTTCGTATAGCCAAGGCATC+TGG 0.430043 6.2:-38049185 MS.gene03518:CDS
TTCAACTCATACAATAAATT+GGG 0.436614 6.2:-38048777 MS.gene03518:CDS
TCGATATGCTTAGCCCTAGC+AGG 0.441225 6.2:+38048267 None:intergenic
CTAAAGCATCAGCCGCCTAC+AGG 0.441391 6.2:-38049215 MS.gene03518:CDS
GGTGCATGCGGTGAGCAATA+TGG 0.455947 6.2:-38048360 MS.gene03518:CDS
CTCTTGTTGTTATTGCATGA+TGG 0.456815 6.2:+38048231 None:intergenic
AATATGGCCCTGATGAATTC+TGG 0.457151 6.2:-38048344 MS.gene03518:CDS
CTCAGAAATGCTTAGCAAGA+TGG 0.474202 6.2:-38049150 MS.gene03518:CDS
TGGGTAATAATACTAATGTT+GGG 0.488773 6.2:-38049103 MS.gene03518:CDS
GAAGAAATTTCTCATCCCTC+TGG 0.489177 6.2:-38048638 MS.gene03518:CDS
ACCAAACATTGAAAATGTAC+TGG 0.490987 6.2:-38048615 MS.gene03518:CDS
ATGGGTACCGAGGATTGGGC+TGG 0.494204 6.2:-38048833 MS.gene03518:CDS
GTGTGAATATCACCCCTGCT+AGG 0.500091 6.2:-38048281 MS.gene03518:CDS
AAGTTGATCTATCTCCATAT+GGG 0.500138 6.2:+38048709 None:intergenic
TGGTAGACTGAATTCCCATA+TGG 0.509370 6.2:-38048723 MS.gene03518:CDS
TAAAGCATCAGCCGCCTACA+GGG 0.517220 6.2:-38049214 MS.gene03518:CDS
CCCATTGCCACACAATGAGT+TGG 0.522267 6.2:+38048851 None:intergenic
TTGCAAATGCCTCTACACTT+CGG 0.528376 6.2:+38049355 None:intergenic
CCATTGCCACACAATGAGTT+GGG 0.530914 6.2:+38048852 None:intergenic
CCCAACTCATTGTGTGGCAA+TGG 0.536825 6.2:-38048852 MS.gene03518:CDS
GTTACCTAAAGACGAGGATG+TGG 0.537974 6.2:-38048430 MS.gene03518:CDS
TCTGTGACATTTGTGAGAGG+TGG 0.541786 6.2:-38048317 MS.gene03518:CDS
TTTGTGAGAGGTGGTTCCAT+GGG 0.542875 6.2:-38048308 MS.gene03518:CDS
GCCAGAGAGAGAACTAATTG+AGG 0.544336 6.2:-38048660 MS.gene03518:CDS
ACACGTAAAACCAGATGCCT+TGG 0.548428 6.2:+38049175 None:intergenic
CCAACTCATTGTGTGGCAAT+GGG 0.564276 6.2:-38048851 MS.gene03518:CDS
TGTGAATATCACCCCTGCTA+GGG 0.567860 6.2:-38048280 MS.gene03518:CDS
AACATTTCCAGCCCAATCCT+CGG 0.568680 6.2:+38048826 None:intergenic
CGATATGCTTAGCCCTAGCA+GGG 0.574696 6.2:+38048268 None:intergenic
TGATATTCACACATTTCCCA+TGG 0.578749 6.2:+38048292 None:intergenic
TGAGTTGAAGACAAAGGACT+AGG 0.578822 6.2:+38048791 None:intergenic
ATTTGTGAGAGGTGGTTCCA+TGG 0.579292 6.2:-38048309 MS.gene03518:CDS
GAGGAAGATGAAGATGAAGA+CGG 0.585144 6.2:-38048396 MS.gene03518:CDS
ATTGTATGAGTTGAAGACAA+AGG 0.596124 6.2:+38048785 None:intergenic
AACAAGAGAGCTCGCCCTTG+AGG 0.603261 6.2:-38048216 None:intergenic
ATCATTATCACCAATAACAA+TGG 0.607015 6.2:+38048748 None:intergenic
GGCACTTGTTCGTATAGCCA+AGG 0.607034 6.2:-38049192 MS.gene03518:CDS
GGAAGAATATGCTTTGGGAA+AGG 0.614050 6.2:-38049072 MS.gene03518:intron
ACTCATACAATAAATTGGGA+TGG 0.616351 6.2:-38048773 MS.gene03518:CDS
TGGATGTGCCCGAAGTGTAG+AGG 0.628364 6.2:-38049364 MS.gene03518:CDS
GGATGTGGAAGAACATTGTG+AGG 0.641770 6.2:-38048415 MS.gene03518:CDS
AAAGCATCAGCCGCCTACAG+GGG 0.652248 6.2:-38049213 MS.gene03518:CDS
CGAACAAGTGCCCCTGTAGG+CGG 0.661590 6.2:+38049203 None:intergenic
TTGTGTGGCAATGGGTACCG+AGG 0.665786 6.2:-38048843 MS.gene03518:CDS
ATATTGCCCAACTCATTGTG+TGG 0.681307 6.2:-38048858 MS.gene03518:CDS
ATACGAACAAGTGCCCCTGT+AGG 0.687555 6.2:+38049200 None:intergenic
AGAAAATCCAGAATTCATCA+GGG 0.689840 6.2:+38048337 None:intergenic
GAAGACGGAGAGAACTCATG+TGG 0.691398 6.2:-38048381 MS.gene03518:CDS
GAGAACTCATGTGGTGCATG+CGG 0.697071 6.2:-38048372 MS.gene03518:CDS
TTTGGAGTTACCTAAAGACG+AGG 0.697923 6.2:-38048436 MS.gene03518:CDS
GATATGCTTAGCCCTAGCAG+GGG 0.762022 6.2:+38048269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAACATGTTTTATGATTT+TGG - chr6.2:38048275-38048294 MS.gene03518:CDS 15.0%
!!! TAATTGATAATAATGCTATA+AGG - chr6.2:38048897-38048916 MS.gene03518:intron 15.0%
!!! TTTTGAATTTTCTCTTAAAT+AGG - chr6.2:38049119-38049138 MS.gene03518:CDS 15.0%
!! TTCAACTCATACAATAAATT+GGG - chr6.2:38048802-38048821 MS.gene03518:CDS 20.0%
!!! TAGATTTTAACACATTGATT+GGG - chr6.2:38049032-38049051 MS.gene03518:intron 20.0%
! AAAGCATATTCTTCCTTTAT+AGG + chr6.2:38048502-38048521 None:intergenic 25.0%
! ATAAAGGAAGAATATGCTTT+GGG - chr6.2:38048502-38048521 MS.gene03518:intron 25.0%
! ATCATTATCACCAATAACAA+TGG + chr6.2:38048834-38048853 None:intergenic 25.0%
! ATGGGTAATAATACTAATGT+TGG - chr6.2:38048475-38048494 MS.gene03518:intron 25.0%
! CATATATCCAACAAGTATTA+AGG + chr6.2:38049081-38049100 None:intergenic 25.0%
! CTTCAACTCATACAATAAAT+TGG - chr6.2:38048801-38048820 MS.gene03518:CDS 25.0%
! GAAAAATTGGAACTTTCTAT+GGG - chr6.2:38048457-38048476 MS.gene03518:intron 25.0%
! TATAAAGGAAGAATATGCTT+TGG - chr6.2:38048501-38048520 MS.gene03518:intron 25.0%
! TGAAAAATTGGAACTTTCTA+TGG - chr6.2:38048456-38048475 MS.gene03518:intron 25.0%
! TGGGTAATAATACTAATGTT+GGG - chr6.2:38048476-38048495 MS.gene03518:intron 25.0%
!! ATTTTCTCTTAAATAGGCTT+TGG - chr6.2:38049125-38049144 MS.gene03518:CDS 25.0%
!! CTTAATACTTGTTGGATATA+TGG - chr6.2:38049079-38049098 MS.gene03518:CDS 25.0%
!! GTTATTGGTGATAATGATAT+TGG - chr6.2:38048836-38048855 MS.gene03518:CDS 25.0%
!! TCTTTATTTGATGCTGAATA+AGG - chr6.2:38048573-38048592 MS.gene03518:CDS 25.0%
!!! ATGTTTTATGATTTTGGTGT+TGG - chr6.2:38048281-38048300 MS.gene03518:CDS 25.0%
!!! CATATTTGTTATTATGCTAC+AGG - chr6.2:38048679-38048698 MS.gene03518:CDS 25.0%
!!! GTAGATTTTAACACATTGAT+TGG - chr6.2:38049031-38049050 MS.gene03518:intron 25.0%
ACAAGTATTAAGGATGTCAT+CGG + chr6.2:38049071-38049090 None:intergenic 30.0%
ACCAAACATTGAAAATGTAC+TGG - chr6.2:38048964-38048983 MS.gene03518:intron 30.0%
ACTCATACAATAAATTGGGA+TGG - chr6.2:38048806-38048825 MS.gene03518:CDS 30.0%
AGAAAATCCAGAATTCATCA+GGG + chr6.2:38049245-38049264 None:intergenic 30.0%
ATGACATCCTTAATACTTGT+TGG - chr6.2:38049071-38049090 MS.gene03518:intron 30.0%
GTGACAGCTACATAAAAATA+GGG + chr6.2:38048544-38048563 None:intergenic 30.0%
TGTGACAGCTACATAAAAAT+AGG + chr6.2:38048545-38048564 None:intergenic 30.0%
TTAACGAAGATTAACGATGT+AGG - chr6.2:38048338-38048357 MS.gene03518:CDS 30.0%
! AAGTTGATCTATCTCCATAT+GGG + chr6.2:38048873-38048892 None:intergenic 30.0%
! ATTGTATGAGTTGAAGACAA+AGG + chr6.2:38048797-38048816 None:intergenic 30.0%
! TCCAGTACATTTTCAATGTT+TGG + chr6.2:38048968-38048987 None:intergenic 30.0%
!! ATTTGTTATTATGCTACAGG+AGG - chr6.2:38048682-38048701 MS.gene03518:CDS 30.0%
!! TTTTAACACATTGATTGGGA+AGG - chr6.2:38049036-38049055 MS.gene03518:intron 30.0%
CAAGATGGAAGCTGAAAAAT+TGG - chr6.2:38048444-38048463 MS.gene03518:intron 35.0%
CAGAAAATCCAGAATTCATC+AGG + chr6.2:38049246-38049265 None:intergenic 35.0%
TACTAATGTTGGGCCTATAA+AGG - chr6.2:38048486-38048505 MS.gene03518:intron 35.0%
TGATATTCACACATTTCCCA+TGG + chr6.2:38049290-38049309 None:intergenic 35.0%
! ATTTTCAATGTTTGGTCCAG+AGG + chr6.2:38048960-38048979 None:intergenic 35.0%
! GAAGTTGATCTATCTCCATA+TGG + chr6.2:38048874-38048893 None:intergenic 35.0%
! TTTTCAATGTTTGGTCCAGA+GGG + chr6.2:38048959-38048978 None:intergenic 35.0%
! TTTTCTGTGACATTTGTGAG+AGG - chr6.2:38049259-38049278 MS.gene03518:intron 35.0%
!! CTCTTGTTGTTATTGCATGA+TGG + chr6.2:38049351-38049370 None:intergenic 35.0%
AATATGGCCCTGATGAATTC+TGG - chr6.2:38049235-38049254 MS.gene03518:intron 40.0%
ATATTGCCCAACTCATTGTG+TGG - chr6.2:38048721-38048740 MS.gene03518:CDS 40.0%
CTCAGAAATGCTTAGCAAGA+TGG - chr6.2:38048429-38048448 MS.gene03518:CDS 40.0%
GAAGAAATTTCTCATCCCTC+TGG - chr6.2:38048941-38048960 MS.gene03518:intron 40.0%
GAGGAAGATGAAGATGAAGA+CGG - chr6.2:38049183-38049202 MS.gene03518:CDS 40.0%
TAGTGTGTGCATGTAAGTTG+TGG - chr6.2:38048250-38048269 MS.gene03518:CDS 40.0%
TGGTAGACTGAATTCCCATA+TGG - chr6.2:38048856-38048875 MS.gene03518:CDS 40.0%
TTGCAAATGCCTCTACACTT+CGG + chr6.2:38048227-38048246 None:intergenic 40.0%
! TCCTCAATTAGTTCTCTCTC+TGG + chr6.2:38048923-38048942 None:intergenic 40.0%
! TGAGTTGAAGACAAAGGACT+AGG + chr6.2:38048791-38048810 None:intergenic 40.0%
! TTTGGAGTTACCTAAAGACG+AGG - chr6.2:38049143-38049162 MS.gene03518:CDS 40.0%
!! AGAATATGCTTTGGGAAAGG+TGG - chr6.2:38048510-38048529 MS.gene03518:intron 40.0%
!! GGAAGAATATGCTTTGGGAA+AGG - chr6.2:38048507-38048526 MS.gene03518:intron 40.0%
AACATTTCCAGCCCAATCCT+CGG + chr6.2:38048756-38048775 None:intergenic 45.0%
ACACGTAAAACCAGATGCCT+TGG + chr6.2:38048407-38048426 None:intergenic 45.0%
ATTTGTGAGAGGTGGTTCCA+TGG - chr6.2:38049270-38049289 MS.gene03518:intron 45.0%
CCAACTCATTGTGTGGCAAT+GGG - chr6.2:38048728-38048747 MS.gene03518:CDS 45.0%
CCATTGCCACACAATGAGTT+GGG + chr6.2:38048730-38048749 None:intergenic 45.0%
GCCAGAGAGAGAACTAATTG+AGG - chr6.2:38048919-38048938 MS.gene03518:intron 45.0%
GGATGTGGAAGAACATTGTG+AGG - chr6.2:38049164-38049183 MS.gene03518:CDS 45.0%
GTTACCTAAAGACGAGGATG+TGG - chr6.2:38049149-38049168 MS.gene03518:CDS 45.0%
TCTGTGACATTTGTGAGAGG+TGG - chr6.2:38049262-38049281 MS.gene03518:intron 45.0%
TCTTCCACATCCTCGTCTTT+AGG + chr6.2:38049156-38049175 None:intergenic 45.0%
TGCAAATGCCTCTACACTTC+GGG + chr6.2:38048226-38048245 None:intergenic 45.0%
TGGGATGGCACCATTGTTAT+TGG - chr6.2:38048821-38048840 MS.gene03518:CDS 45.0%
TGTGAATATCACCCCTGCTA+GGG - chr6.2:38049299-38049318 MS.gene03518:intron 45.0%
TTTGTGAGAGGTGGTTCCAT+GGG - chr6.2:38049271-38049290 MS.gene03518:intron 45.0%
ATACGAACAAGTGCCCCTGT+AGG + chr6.2:38048382-38048401 None:intergenic 50.0%
CCCAACTCATTGTGTGGCAA+TGG - chr6.2:38048727-38048746 MS.gene03518:CDS 50.0%
CCCATTGCCACACAATGAGT+TGG + chr6.2:38048731-38048750 None:intergenic 50.0%
CGATATGCTTAGCCCTAGCA+GGG + chr6.2:38049314-38049333 None:intergenic 50.0%
GAAGACGGAGAGAACTCATG+TGG - chr6.2:38049198-38049217 MS.gene03518:CDS 50.0%
GAGAACTCATGTGGTGCATG+CGG - chr6.2:38049207-38049226 MS.gene03518:CDS 50.0%
GATATGCTTAGCCCTAGCAG+GGG + chr6.2:38049313-38049332 None:intergenic 50.0%
GGCACTTGTTCGTATAGCCA+AGG - chr6.2:38048387-38048406 MS.gene03518:CDS 50.0%
GTGTGAATATCACCCCTGCT+AGG - chr6.2:38049298-38049317 MS.gene03518:intron 50.0%
TCGATATGCTTAGCCCTAGC+AGG + chr6.2:38049315-38049334 None:intergenic 50.0%
! GTTCGTATAGCCAAGGCATC+TGG - chr6.2:38048394-38048413 MS.gene03518:CDS 50.0%
! TAAAGCATCAGCCGCCTACA+GGG - chr6.2:38048365-38048384 MS.gene03518:CDS 50.0%
GGTGCATGCGGTGAGCAATA+TGG - chr6.2:38049219-38049238 MS.gene03518:CDS 55.0%
! AAAGCATCAGCCGCCTACAG+GGG - chr6.2:38048366-38048385 MS.gene03518:CDS 55.0%
! CTAAAGCATCAGCCGCCTAC+AGG - chr6.2:38048364-38048383 MS.gene03518:CDS 55.0%
! TTGTGTGGCAATGGGTACCG+AGG - chr6.2:38048736-38048755 MS.gene03518:CDS 55.0%
!! GGCAATGGGTACCGAGGATT+GGG - chr6.2:38048742-38048761 MS.gene03518:CDS 55.0%
!! TGGCAATGGGTACCGAGGAT+TGG - chr6.2:38048741-38048760 MS.gene03518:CDS 55.0%
CGAACAAGTGCCCCTGTAGG+CGG + chr6.2:38048379-38048398 None:intergenic 60.0%
!! ATGGGTACCGAGGATTGGGC+TGG - chr6.2:38048746-38048765 MS.gene03518:CDS 60.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 38048218 38049383 38048218 ID=MS.gene03518
chr6.2 mRNA 38048218 38049383 38048218 ID=MS.gene03518.t1;Parent=MS.gene03518
chr6.2 exon 38049340 38049383 38049340 ID=MS.gene03518.t1.exon1;Parent=MS.gene03518.t1
chr6.2 CDS 38049340 38049383 38049340 ID=cds.MS.gene03518.t1;Parent=MS.gene03518.t1
chr6.2 exon 38049073 38049241 38049073 ID=MS.gene03518.t1.exon2;Parent=MS.gene03518.t1
chr6.2 CDS 38049073 38049241 38049073 ID=cds.MS.gene03518.t1;Parent=MS.gene03518.t1
chr6.2 exon 38048544 38048900 38048544 ID=MS.gene03518.t1.exon3;Parent=MS.gene03518.t1
chr6.2 CDS 38048544 38048900 38048544 ID=cds.MS.gene03518.t1;Parent=MS.gene03518.t1
chr6.2 exon 38048218 38048460 38048218 ID=MS.gene03518.t1.exon4;Parent=MS.gene03518.t1
chr6.2 CDS 38048218 38048460 38048218 ID=cds.MS.gene03518.t1;Parent=MS.gene03518.t1
Gene Sequence

>MS.gene03518

ATGTGCCCGAAGTGTAGAGGCATTTGCAACTGTAGTGTGTGCATGTAAGTTGTGGTTTAAAACATGTTTTATGATTTTGGTGTTGGAGTTTTCATTTTGTAACATTTTGTGTATTAGATCTTAACGAAGATTAACGATGTAGGAGACTAAAGCATCAGCCGCCTACAGGGGCACTTGTTCGTATAGCCAAGGCATCTGGTTTTACGTGTGTCTCAGAAATGCTTAGCAAGATGGAAGCTGAAAAATTGGAACTTTCTATGGGTAATAATACTAATGTTGGGCCTATAAAGGAAGAATATGCTTTGGGAAAGGTGGTTTTCTTTCCCTATTTTTATGTAGCTGTCACAATAAGTTTTCTTTATTTGATGCTGAATAAGGCTCTTTTTATTTATTCAATTTCAAATAATGATAAAAAATGTAAGAGTGTGCTGATTATCATTGTATTTTAAATATTTGAATTTCATATTTGTTATTATGCTACAGGAGGTTTTAGTAGAAGAGAAATATTGCCCAACTCATTGTGTGGCAATGGGTACCGAGGATTGGGCTGGAAATGTTTTATTTTCTGATCCTAGTCCTTTGTCTTCAACTCATACAATAAATTGGGATGGCACCATTGTTATTGGTGATAATGATATTGGTAGACTGAATTCCCATATGGAGATAGATCAACTTCAAATAATTGATAATAATGCTATAAGGCCAGAGAGAGAACTAATTGAGGAAGAAATTTCTCATCCCTCTGGACCAAACATTGAAAATGTACTGGACGTTGAATTTCAACGAGAAGATGATGCGAGTGAAATGCAGTTTGTAGATTTTAACACATTGATTGGGAAGGTACGCATTTCCGATGACATCCTTAATACTTGTTGGATATATGGTAGTTATGTGTTTATAATTTTGAATTTTCTCTTAAATAGGCTTTGGAGTTACCTAAAGACGAGGATGTGGAAGAACATTGTGAGGAAGATGAAGATGAAGACGGAGAGAACTCATGTGGTGCATGCGGTGAGCAATATGGCCCTGATGAATTCTGGATTTTCTGTGACATTTGTGAGAGGTGGTTCCATGGGAAATGTGTGAATATCACCCCTGCTAGGGCTAAGCATATCGAGATATACAAGTGTCCATCATGCAATAACAACAAGAGAGCTCGCCCTTGA
Protein sequence

>MS.gene03518.t1

MCPKCRGICNCSVCMRLKHQPPTGALVRIAKASGFTCVSEMLSKMEAEKLELSMGNNTNVGPIKEEYALGKEVLVEEKYCPTHCVAMGTEDWAGNVLFSDPSPLSSTHTINWDGTIVIGDNDIGRLNSHMEIDQLQIIDNNAIRPERELIEEEISHPSGPNIENVLDVEFQREDDASEMQFVDFNTLIGKALELPKDEDVEEHCEEDEDEDGENSCGACGEQYGPDEFWIFCDICERWFHGKCVNITPARAKHIEIYKCPSCNNNKRARP