Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035285.t1 | XP_003609586.1 | 91.2 | 181 | 16 | 0 | 1 | 181 | 1 | 181 | 2.50E-78 | 301.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035285.t1 | G7JJ33 | 91.2 | 181 | 16 | 0 | 1 | 181 | 1 | 181 | 1.8e-78 | 301.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene035285.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035285.t1 | MTR_4g118830 | 91.160 | 181 | 16 | 0 | 1 | 181 | 1 | 181 | 2.05e-116 | 328 |
| MS.gene035285.t1 | MTR_4g118430 | 45.946 | 111 | 41 | 3 | 77 | 181 | 1 | 98 | 3.27e-21 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTCTCTGTTACGGTTAC+TGG | 0.183266 | 8.1:-9075643 | MS.gene035285:CDS |
| AGTAACCGTAACAGAGAAAT+TGG | 0.195649 | 8.1:+9075645 | None:intergenic |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.266041 | 8.1:-9075619 | MS.gene035285:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8.1:-9075672 | MS.gene035285:CDS |
| GAGAGAGATTATGAGTTTGT+AGG | 0.295578 | 8.1:-9075434 | None:intergenic |
| ATCACTGCTTTGGTTCAAAA+TGG | 0.315294 | 8.1:-9075478 | MS.gene035285:CDS |
| TAACAAATCAGTTTCTGAAA+TGG | 0.363545 | 8.1:-9076081 | MS.gene035285:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8.1:-9075488 | MS.gene035285:CDS |
| AACAAATCAGTTTCTGAAAT+GGG | 0.410347 | 8.1:-9076080 | MS.gene035285:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8.1:+9075952 | None:intergenic |
| GAATCCCAATTTCTCTGTTA+CGG | 0.433893 | 8.1:-9075650 | MS.gene035285:CDS |
| TAATGATGGTAATTTGATCT+TGG | 0.443216 | 8.1:-9075503 | MS.gene035285:CDS |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8.1:-9075734 | MS.gene035285:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8.1:+9075951 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8.1:-9075704 | MS.gene035285:CDS |
| TGAAGAAAAGAAGAAAATGT+TGG | 0.489430 | 8.1:-9075746 | MS.gene035285:CDS |
| GCTAATGTTTCTGTTAATGA+TGG | 0.496957 | 8.1:-9075517 | MS.gene035285:CDS |
| TTTCTTTGGCATTGAATCTG+TGG | 0.500456 | 8.1:-9075605 | MS.gene035285:CDS |
| TTCAACGTTTAGAACCTCTT+GGG | 0.500668 | 8.1:+9076028 | None:intergenic |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8.1:+9075967 | None:intergenic |
| GTTCAACGTTTAGAACCTCT+TGG | 0.509820 | 8.1:+9076027 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8.1:-9075595 | MS.gene035285:CDS |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8.1:-9075706 | MS.gene035285:CDS |
| AGAAAAGAAGAAAATGTTGG+AGG | 0.526690 | 8.1:-9075743 | MS.gene035285:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8.1:-9075705 | MS.gene035285:CDS |
| GTAACCGTAACAGAGAAATT+GGG | 0.551700 | 8.1:+9075646 | None:intergenic |
| TAAACGTTGAACGTGAAAGA+AGG | 0.570116 | 8.1:-9076016 | MS.gene035285:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8.1:-9075703 | MS.gene035285:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8.1:+9075577 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8.1:-9075629 | MS.gene035285:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8.1:+9075959 | None:intergenic |
| TACACAGGAAGTGATTGTGA+AGG | 0.673700 | 8.1:-9075794 | MS.gene035285:CDS |
| TTGGGATTCCTATTACGACA+CGG | 0.678110 | 8.1:+9075664 | None:intergenic |
| CACTACTACTAAAACCCAAG+AGG | 0.682406 | 8.1:-9076042 | MS.gene035285:CDS |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8.1:-9076065 | MS.gene035285:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAATAAAAGAGAAAA+TGG | + | chr8.1:9075619-9075638 | None:intergenic | 15.0% |
| !! | ATAACAAATCAAACTATAAT+GGG | + | chr8.1:9075691-9075710 | None:intergenic | 15.0% |
| !!! | TTTATTTTCATTCTTCATTT+GGG | - | chr8.1:9075627-9075646 | MS.gene035285:CDS | 15.0% |
| !!! | TTTTATTTTCATTCTTCATT+TGG | - | chr8.1:9075626-9075645 | MS.gene035285:CDS | 15.0% |
| !! | AAAATAAAAGAGAAAATGGA+AGG | + | chr8.1:9075615-9075634 | None:intergenic | 20.0% |
| !! | GATAACAAATCAAACTATAA+TGG | + | chr8.1:9075692-9075711 | None:intergenic | 20.0% |
| !! | TGCATTTAAACATGAAAAAT+TGG | + | chr8.1:9075656-9075675 | None:intergenic | 20.0% |
| !!! | TTTGATTTGTTATCTGAAAT+GGG | - | chr8.1:9075698-9075717 | MS.gene035285:CDS | 20.0% |
| ! | TAATGATGGTAATTTGATCT+TGG | - | chr8.1:9076027-9076046 | MS.gene035285:CDS | 25.0% |
| ! | TGAAGAAAAGAAGAAAATGT+TGG | - | chr8.1:9075784-9075803 | MS.gene035285:CDS | 25.0% |
| !! | TAACAAATCAGTTTCTGAAA+TGG | - | chr8.1:9075449-9075468 | MS.gene035285:CDS | 25.0% |
| !! | TTTGATTACTGTGATTTTGA+AGG | - | chr8.1:9075958-9075977 | MS.gene035285:CDS | 25.0% |
| !!! | AACAAATCAGTTTCTGAAAT+GGG | - | chr8.1:9075450-9075469 | MS.gene035285:CDS | 25.0% |
| !!! | GTTTGATTTGTTATCTGAAA+TGG | - | chr8.1:9075697-9075716 | MS.gene035285:CDS | 25.0% |
| !!! | TGAAATGGGTTTTTGTTTTT+AGG | - | chr8.1:9075712-9075731 | MS.gene035285:CDS | 25.0% |
| AAAATCACAGTAATCAAACC+AGG | + | chr8.1:9075956-9075975 | None:intergenic | 30.0% | |
| AAATCAAAGAAACCTGTTGA+AGG | - | chr8.1:9075824-9075843 | MS.gene035285:intron | 30.0% | |
| AATCAAAGAAACCTGTTGAA+GGG | - | chr8.1:9075825-9075844 | MS.gene035285:intron | 30.0% | |
| AGAAAAGAAGAAAATGTTGG+AGG | - | chr8.1:9075787-9075806 | MS.gene035285:CDS | 30.0% | |
| AGGAATGATGTTAGTTACAT+TGG | + | chr8.1:9075595-9075614 | None:intergenic | 30.0% | |
| GAATGATGTTAGTTACATTG+GGG | + | chr8.1:9075593-9075612 | None:intergenic | 30.0% | |
| GGAATGATGTTAGTTACATT+GGG | + | chr8.1:9075594-9075613 | None:intergenic | 30.0% | |
| ! | GCTAATGTTTCTGTTAATGA+TGG | - | chr8.1:9076013-9076032 | MS.gene035285:CDS | 30.0% |
| !!! | TTTTTGTTTTTAGGCTACAC+AGG | - | chr8.1:9075721-9075740 | MS.gene035285:CDS | 30.0% |
| AAATTAAAACCCCCTTCAAC+AGG | + | chr8.1:9075839-9075858 | None:intergenic | 35.0% | |
| AATTTCTCTGTTACGGTTAC+TGG | - | chr8.1:9075887-9075906 | MS.gene035285:intron | 35.0% | |
| AGTAACCGTAACAGAGAAAT+TGG | + | chr8.1:9075888-9075907 | None:intergenic | 35.0% | |
| ATCAAAGAAACCTGTTGAAG+GGG | - | chr8.1:9075826-9075845 | MS.gene035285:intron | 35.0% | |
| GAATCCCAATTTCTCTGTTA+CGG | - | chr8.1:9075880-9075899 | MS.gene035285:intron | 35.0% | |
| GTAACCGTAACAGAGAAATT+GGG | + | chr8.1:9075887-9075906 | None:intergenic | 35.0% | |
| TAAACGTTGAACGTGAAAGA+AGG | - | chr8.1:9075514-9075533 | MS.gene035285:CDS | 35.0% | |
| ! | ATCACTGCTTTGGTTCAAAA+TGG | - | chr8.1:9076052-9076071 | MS.gene035285:CDS | 35.0% |
| ! | TTCAACGTTTAGAACCTCTT+GGG | + | chr8.1:9075505-9075524 | None:intergenic | 35.0% |
| ! | TTTCTTTGGCATTGAATCTG+TGG | - | chr8.1:9075925-9075944 | MS.gene035285:intron | 35.0% |
| !! | GTTTTTGAAGAATGAAGCTG+AGG | - | chr8.1:9075982-9076001 | MS.gene035285:CDS | 35.0% |
| !!! | TGAAGGGGGTTTTAATTTGT+TGG | - | chr8.1:9075841-9075860 | MS.gene035285:intron | 35.0% |
| ATTGAATCTGTGGCTAAACC+TGG | - | chr8.1:9075935-9075954 | MS.gene035285:intron | 40.0% | |
| CACTACTACTAAAACCCAAG+AGG | - | chr8.1:9075488-9075507 | MS.gene035285:CDS | 40.0% | |
| GAAAATGTTGGAGGAGATGA+AGG | - | chr8.1:9075796-9075815 | MS.gene035285:CDS | 40.0% | |
| GAAATGGGTAACAGAAAACG+TGG | - | chr8.1:9075465-9075484 | MS.gene035285:CDS | 40.0% | |
| TACACAGGAAGTGATTGTGA+AGG | - | chr8.1:9075736-9075755 | MS.gene035285:CDS | 40.0% | |
| TCAAAGAAACCTGTTGAAGG+GGG | - | chr8.1:9075827-9075846 | MS.gene035285:intron | 40.0% | |
| TCAAGCAATGTGGCTTTCTT+TGG | - | chr8.1:9075911-9075930 | MS.gene035285:intron | 40.0% | |
| TTACATTGGGGTAGAGAGTT+GGG | + | chr8.1:9075581-9075600 | None:intergenic | 40.0% | |
| TTGGGATTCCTATTACGACA+CGG | + | chr8.1:9075869-9075888 | None:intergenic | 40.0% | |
| ! | GTTCAACGTTTAGAACCTCT+TGG | + | chr8.1:9075506-9075525 | None:intergenic | 40.0% |
| GTTACATTGGGGTAGAGAGT+TGG | + | chr8.1:9075582-9075601 | None:intergenic | 45.0% | |
| !! | GGTTACTGGTTCAAGCAATG+TGG | - | chr8.1:9075901-9075920 | MS.gene035285:intron | 45.0% |
| !! | TGTTGGTTCCGTGTCGTAAT+AGG | - | chr8.1:9075858-9075877 | MS.gene035285:intron | 45.0% |
| GATCTTGGCGATCACTGCTT+TGG | - | chr8.1:9076042-9076061 | MS.gene035285:CDS | 50.0% | |
| ! | GAGTTGGGACAGTGGTAGTG+AGG | + | chr8.1:9075566-9075585 | None:intergenic | 55.0% |
| GGGGTAGAGAGTTGGGACAG+TGG | + | chr8.1:9075574-9075593 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 9075435 | 9076117 | 9075435 | ID=MS.gene035285 |
| chr8.1 | mRNA | 9075435 | 9076117 | 9075435 | ID=MS.gene035285.t1;Parent=MS.gene035285 |
| chr8.1 | exon | 9075956 | 9076117 | 9075956 | ID=MS.gene035285.t1.exon1;Parent=MS.gene035285.t1 |
| chr8.1 | CDS | 9075956 | 9076117 | 9075956 | ID=cds.MS.gene035285.t1;Parent=MS.gene035285.t1 |
| chr8.1 | exon | 9075435 | 9075818 | 9075435 | ID=MS.gene035285.t1.exon2;Parent=MS.gene035285.t1 |
| chr8.1 | CDS | 9075435 | 9075818 | 9075435 | ID=cds.MS.gene035285.t1;Parent=MS.gene035285.t1 |
| Gene Sequence |
| Protein sequence |
