AlfalfaGEDB | MS.gene03529

M. sativa cultivar XinJiangDaYe / MS.gene03529

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03529.t1	XP_013459264.1	93.7	303	19	0	1	303	1	303	5.10E-169	603.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03529.t1	Q9FLJ2	56.7	337	111	5	1	303	1	336	3.3e-100	366.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03529.t1	A0A072V531	93.7	303	19	0	1	303	1	303	3.6e-169	603.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene03529.t1	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03529.t1	MTR_3g435150	93.729	303	19	0	1	303	1	303	0.0	594
MS.gene03529.t1	MTR_4g108760	67.183	323	80	7	1	302	1	318	4.68e-144	410
MS.gene03529.t1	MTR_6g012670	52.795	322	128	9	1	303	5	321	8.45e-102	301
MS.gene03529.t1	MTR_7g085260	66.204	216	64	6	1	208	1	215	9.41e-92	278
MS.gene03529.t1	MTR_6g032770	63.285	207	70	5	6	207	8	213	4.68e-88	268
MS.gene03529.t1	MTR_6g084430	59.908	217	80	3	16	231	5	215	9.27e-88	265
MS.gene03529.t1	MTR_2g078700	64.352	216	55	3	16	209	23	238	7.08e-87	265
MS.gene03529.t1	MTR_7g011130	50.649	308	112	9	2	282	5	299	4.46e-82	252
MS.gene03529.t1	MTR_3g070030	61.650	206	75	2	2	203	5	210	4.65e-82	252
MS.gene03529.t1	MTR_7g011120	61.735	196	65	2	10	195	2	197	7.35e-77	237

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene03529.t1	AT5G61430	56.677	337	111	5	1	303	1	336	1.14e-126	365
MS.gene03529.t1	AT5G07680	55.132	341	103	9	1	303	1	329	7.79e-118	342
MS.gene03529.t1	AT5G07680	55.352	327	97	8	14	303	1	315	1.91e-114	333
MS.gene03529.t1	AT5G39610	68.224	214	65	1	4	217	8	218	2.57e-100	296
MS.gene03529.t1	AT3G29035	70.256	195	44	1	8	202	16	196	3.91e-93	279
MS.gene03529.t1	AT3G04060	66.176	204	62	4	2	198	4	207	3.59e-90	272
MS.gene03529.t1	AT5G18270	57.200	250	91	4	11	244	16	265	7.37e-90	271
MS.gene03529.t1	AT5G18270	56.800	250	92	4	11	244	16	265	4.83e-89	269
MS.gene03529.t1	AT5G61430	52.747	273	94	5	65	303	2	273	1.02e-88	266
MS.gene03529.t1	AT3G15170	59.111	225	80	4	11	230	15	232	2.80e-86	261

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Find 56 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTACCCAACTGGTTTG+AGG	0.496887	3.1:-27543002	MS.gene03529:CDS
AAAGATAAGGAGATTTACAG+AGG	0.584344	3.1:-27542937	MS.gene03529:CDS
AACTTACATGGCAAATCCCA+AGG	0.588889	3.1:+27543300	None:intergenic
AATGCATGAATATAGACTTG+AGG	0.610990	3.1:-27542836	MS.gene03529:CDS
ACTAATAGAGCTACTGAAGC+TGG	0.478520	3.1:-27542979	MS.gene03529:CDS
AGATTGAGTGGGTGATTTGC+AGG	0.374745	3.1:-27542331	MS.gene03529:CDS
AGATTTAGTGAATGATTTGA+AGG	0.460614	3.1:+27542083	None:intergenic
AGGAATCAAAGGTTCCAACT+TGG	0.513759	3.1:+27542129	None:intergenic
AGGTAATGGCAAATTTGTTG+AGG	0.565495	3.1:+27542001	None:intergenic
ATAATGAGGTTAGAATCTTT+TGG	0.317375	3.1:-27542263	MS.gene03529:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGATAAGGAGATTTACAG+AGG	-	chr3.1:27542439-27542458	MS.gene03529:intron	30.0%
AATGCATGAATATAGACTTG+AGG	-	chr3.1:27542540-27542559	MS.gene03529:intron	30.0%
AGGACTCATAATAACTTACA+TGG	+	chr3.1:27542091-27542110	None:intergenic	30.0%
ATGCATGAATATAGACTTGA+GGG	-	chr3.1:27542541-27542560	MS.gene03529:intron	30.0%
CAATGATCAAAAACACAAGT+AGG	+	chr3.1:27542111-27542130	None:intergenic	30.0%
CAATTGATCTATCTACCTTA+TGG	-	chr3.1:27543462-27543481	MS.gene03529:CDS	30.0%
CATAAGGTAGATAGATCAAT+TGG	+	chr3.1:27543464-27543483	None:intergenic	30.0%
CTAAAGGAGAGAAAAGTAAT+TGG	-	chr3.1:27542515-27542534	MS.gene03529:intron	30.0%
GATTTGAAGGAACTTCAAAA+TGG	+	chr3.1:27543283-27543302	None:intergenic	30.0%
GTTATAGGTAAAGCCAAAAT+GGG	-	chr3.1:27542316-27542335	MS.gene03529:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	27541905	27543493	27541905	ID=MS.gene03529
chr3.1	mRNA	27541905	27543493	27541905	ID=MS.gene03529.t1;Parent=MS.gene03529
chr3.1	exon	27543307	27543493	27543307	ID=MS.gene03529.t1.exon1;Parent=MS.gene03529.t1
chr3.1	CDS	27543307	27543493	27543307	ID=cds.MS.gene03529.t1;Parent=MS.gene03529.t1
chr3.1	exon	27542798	27543075	27542798	ID=MS.gene03529.t1.exon2;Parent=MS.gene03529.t1
chr3.1	CDS	27542798	27543075	27542798	ID=cds.MS.gene03529.t1;Parent=MS.gene03529.t1
chr3.1	exon	27541905	27542351	27541905	ID=MS.gene03529.t1.exon3;Parent=MS.gene03529.t1
chr3.1	CDS	27541905	27542351	27541905	ID=cds.MS.gene03529.t1;Parent=MS.gene03529.t1

Gene Sequence

>MS.gene03529

ATGGAAAATATTTCAGTTTTGTGTAAGGAAGAGGATCAGATGATTTTGCCTCCAGGGTTTAGGTTTCATCCAACTGATGAAGAGCTTATTAGTCATTACCTTTATAAAAAGGTAATTGACATAAACTTTTCTGCTAGGGCCATTGGAGAGGTTGATTTGAACAAATCTGAGCCTTGGGATTTGCCATGTAAGTTATTATGAGTCCTACTTGTGTTTTTGATCATTGATTTAATTCATATGCACGTAGATCGGGCAATTATATCTGTCTGATCATTAAAAATGTTTGACTTTCATCGCATGTAAATGATAGGTTTATATTAGGTTTCATAAAAAATATTCATTTTATTTTTATTGTGTTTGTAAAATGTAATTATTTTCTTTTTTTGTTACTTTTATGATATGATAAATTTGGTTATAGGTAAAGCCAAAATGGGTGAAAAAGAGTGGTATTTTTTTTGTGTGAGAGACAAAAAGTACCCAACTGGTTTGAGGACTAATAGAGCTACTGAAGCTGGATATTGGAAGGCAACTGGAAAAGATAAGGAGATTTACAGAGGAAAAGCTCTTGTTGGAATGAAGAAAACTCTTGTTTTCTACAAAGGAAGAGCACCTAAAGGAGAGAAAAGTAATTGGGTAATGCATGAATATAGACTTGAGGGTAAATTCTCTGTTTACAACCTCCCTAAAACTGCCAAGGTAAATTCTCTATTCCAAAAATGTATACCTATTTTTTTCGGCTTTAAATAAAGGTCTGCGATCGTAGTATAAGCCGTGGCATAAGGCTAATGTGATTGCAATTGAGGTCGCATCGACTACAATTGAGTTACAATCGCAATGATGCGATTGCAAACCTTACTAAGACCACTTTTAAAAATGTTGGTTGTGAATTTTTGTTCATTACTATTCTCTATAGCACCAACACATTTGATCTAAGGCGTGTCTGGACTCTCACATGTGTCTGTGTTCGAAACCAACATGACATGTGTTTGTGTCCAAAACCAACACGACCTGTTACATGTGATTACATTCAATTGATTTATTATTTGAAATTATCATTACTCATTAGTGTTGACCTGCAAGGGTCAGTGTCGTGTTGTGTCAGTGCTTCATTTCGACTAATTGGTTTTGGTCTTTTGTGGCAGATTGAGTGGGTGATTTGCAGGGTATTCAAGAAGAATTTATCAGGCAAAAAGACATGTCATTCTGGGATAATGAGGTTAGAATCTTTTGGAAATGAAATGAGTTCTTCTCTTCTTCCACCACTCACAGATTCATCACCTTATTCCATTGACAAAATCAAATCACCTTACGTGTCCTGCTTCTCCAATCCAATTGAAAGAAACCAAGTTGGAACCTTTGATTCCTTTAACAACACCCATTTTGAAGTTCCTTCAAATCATTCACTAAATCTTCCAAAAAATACACTTTCTATTGGTTCATTTTACTCTACTCAAGGAATTCAAGTTCATCCCTCAACAAATTTGCCATTACCTGGTTCTGTCTATAACAATGGATCAAACTTTGATAATCAGCACCATCAACCTGCTTCAACTGCACCAATTGATCTATCTACCTTATGGAATTACTGA

Protein sequence

>MS.gene03529.t1

MENISVLCKEEDQMILPPGFRFHPTDEELISHYLYKKVIDINFSARAIGEVDLNKSEPWDLPCKAKMGEKEWYFFCVRDKKYPTGLRTNRATEAGYWKATGKDKEIYRGKALVGMKKTLVFYKGRAPKGEKSNWVMHEYRLEGKFSVYNLPKTAKIEWVICRVFKKNLSGKKTCHSGIMRLESFGNEMSSSLLPPLTDSSPYSIDKIKSPYVSCFSNPIERNQVGTFDSFNNTHFEVPSNHSLNLPKNTLSIGSFYSTQGIQVHPSTNLPLPGSVYNNGSNFDNQHHQPASTAPIDLSTLWNY