AlfalfaGEDB | MS.gene035320

M. sativa cultivar XinJiangDaYe / MS.gene035320

KEGG: K08065
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0046982 GO:0046983 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0110165 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035320.t1	AFK36846.1	97.7	129	3	0	1	129	1	129	3.80E-65	257.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035320.t1	O82248	78.9	95	20	0	22	116	51	145	5.2e-39	161.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035320.t1	G7JKC3	97.7	129	3	0	1	129	1	129	2.7e-65	257.3

TFs/TRs:

Gene ID	Type	Classification
MS.gene035320.t1	TF	NF-YB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035320.t1	MTR_4g119500	97.674	129	3	0	1	129	1	129	6.30e-93	264
MS.gene035320.t1	MTR_0392s0020	86.614	127	12	4	2	128	1	122	1.32e-71	211
MS.gene035320.t1	MTR_1g088860	74.107	112	29	0	5	116	19	130	4.40e-61	186
MS.gene035320.t1	MTR_4g133938	67.273	110	31	1	12	116	4	113	7.11e-51	158
MS.gene035320.t1	MTR_1g072790	66.990	103	34	0	21	123	28	130	6.70e-50	157
MS.gene035320.t1	MTR_2g026710	65.138	109	35	1	12	117	4	112	2.78e-49	155
MS.gene035320.t1	MTR_7g061270	62.162	111	40	1	7	115	9	119	1.04e-48	154
MS.gene035320.t1	MTR_7g100650	62.162	111	40	1	7	115	9	119	1.68e-48	153
MS.gene035320.t1	MTR_8g091720	62.385	109	41	0	21	129	27	135	4.04e-48	153
MS.gene035320.t1	MTR_3g058980	67.010	97	32	0	21	117	26	122	5.50e-48	152
MS.gene035320.t1	MTR_5g095740	67.010	97	32	0	21	117	22	118	1.09e-47	151
MS.gene035320.t1	MTR_1g083070	72.632	95	26	0	22	116	4	98	1.54e-47	149
MS.gene035320.t1	MTR_8g093920	69.892	93	28	0	21	113	39	131	3.89e-47	151
MS.gene035320.t1	MTR_4g133952	60.000	110	44	0	19	128	55	164	4.97e-47	152
MS.gene035320.t1	MTR_1g039040	63.830	94	34	0	20	113	4	97	3.42e-42	138
MS.gene035320.t1	MTR_1g029070	47.872	94	49	0	21	114	51	144	2.06e-26	97.4
MS.gene035320.t1	MTR_5g095900	46.237	93	50	0	19	111	13	105	4.94e-25	94.0
MS.gene035320.t1	MTR_1g028480	44.304	79	39	1	47	125	14	87	1.03e-18	75.9
MS.gene035320.t1	MTR_1g029100	36.250	80	51	0	27	106	7	86	5.71e-17	70.9
MS.gene035320.t1	MTR_2g056000	32.955	88	59	0	23	110	42	129	6.63e-16	70.5
MS.gene035320.t1	MTR_4g112380	30.682	88	61	0	23	110	12	99	4.38e-15	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene035320.t1	AT2G47810	77.551	98	22	0	19	116	48	145	1.03e-55	171
MS.gene035320.t1	AT2G38880	69.307	101	31	0	15	115	14	114	3.44e-51	159
MS.gene035320.t1	AT2G38880	69.307	101	31	0	15	115	14	114	4.72e-51	159
MS.gene035320.t1	AT2G38880	69.307	101	31	0	15	115	14	114	4.72e-51	159
MS.gene035320.t1	AT2G38880	69.307	101	31	0	15	115	14	114	4.72e-51	159
MS.gene035320.t1	AT4G14540	60.000	125	46	2	3	127	5	125	1.33e-50	158
MS.gene035320.t1	AT1G09030	71.429	98	28	0	20	117	1	98	7.14e-50	155
MS.gene035320.t1	AT5G47640	66.667	108	32	1	21	124	26	133	1.28e-49	157
MS.gene035320.t1	AT2G38880	68.367	98	31	0	15	112	14	111	1.82e-49	154
MS.gene035320.t1	AT2G38880	69.072	97	30	0	15	111	14	110	3.34e-49	154
MS.gene035320.t1	AT2G38880	68.367	98	31	0	15	112	14	111	6.55e-49	154
MS.gene035320.t1	AT2G38880	68.367	98	31	0	15	112	14	111	6.55e-49	154
MS.gene035320.t1	AT3G53340	66.337	101	34	0	20	120	27	127	7.51e-49	153
MS.gene035320.t1	AT3G53340	66.337	101	34	0	20	120	27	127	7.51e-49	153
MS.gene035320.t1	AT2G38880	68.367	98	31	0	15	112	14	111	7.99e-49	153
MS.gene035320.t1	AT2G38880	68.367	98	31	0	15	112	14	111	7.99e-49	153
MS.gene035320.t1	AT2G38880	68.367	98	31	0	15	112	14	111	7.99e-49	153
MS.gene035320.t1	AT3G53340	68.750	96	30	0	20	115	27	122	2.03e-48	153
MS.gene035320.t1	AT3G53340	68.750	96	30	0	20	115	27	122	2.03e-48	153
MS.gene035320.t1	AT2G37060	61.682	107	41	0	20	126	28	134	7.66e-48	152
MS.gene035320.t1	AT2G37060	61.682	107	41	0	20	126	28	134	7.66e-48	152
MS.gene035320.t1	AT2G37060	61.682	107	41	0	20	126	28	134	7.66e-48	152
MS.gene035320.t1	AT3G53340	68.817	93	29	0	20	112	27	119	2.80e-47	149
MS.gene035320.t1	AT2G13570	59.829	117	47	0	1	117	15	131	4.57e-47	151
MS.gene035320.t1	AT5G47670	61.905	105	36	1	16	116	19	123	6.59e-45	145
MS.gene035320.t1	AT5G47670	61.905	105	36	1	16	116	48	152	1.34e-44	145
MS.gene035320.t1	AT5G47670	61.905	105	36	1	16	116	48	152	1.34e-44	145
MS.gene035320.t1	AT1G21970	52.632	114	45	1	12	116	40	153	1.36e-40	135
MS.gene035320.t1	AT5G08190	30.612	98	66	1	22	117	11	108	1.21e-15	69.3
MS.gene035320.t1	AT5G08190	30.851	94	63	1	22	113	11	104	3.97e-15	68.2
MS.gene035320.t1	AT5G23090	30.928	97	65	1	23	117	12	108	4.35e-15	67.8
MS.gene035320.t1	AT5G23090	31.183	93	62	1	23	113	12	104	2.79e-14	65.9
MS.gene035320.t1	AT5G23090	31.183	93	62	1	23	113	12	104	2.79e-14	65.9
MS.gene035320.t1	AT5G23090	31.183	93	62	1	23	113	12	104	2.79e-14	65.9

Find 27 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGATATCTGTTGGGCTTT+GGG	0.254012	8.3:+7418844	MS.gene035320:CDS
TGATGATATCTGTTGGGCTT+TGG	0.268785	8.3:+7418843	MS.gene035320:CDS
AGAAATTAATCTCCTCTATC+TGG	0.292960	8.3:-7418967	None:intergenic
GCATAGATATAGAGAATTAG+AGG	0.332962	8.3:+7418912	MS.gene035320:CDS
ATCCTCCCAACATTTGCTAT+TGG	0.373158	8.3:-7418676	None:intergenic
TGTTGGGCTTTGGGGACACT+TGG	0.405725	8.3:+7418853	MS.gene035320:CDS
GGAATATTATTAGCAGTAGT+TGG	0.426129	8.3:-7418946	None:intergenic
CTATGCAAATACCTTCTCAT+TGG	0.433135	8.3:-7418895	None:intergenic
GCTAATAATATTCCAGATAG+AGG	0.450061	8.3:+7418955	MS.gene035320:CDS
TGAAAACGGAGGCATAATAA+AGG	0.463124	8.3:+7418636	MS.gene035320:CDS
GCATAATAAAGGAACAAGAT+AGG	0.472463	8.3:+7418647	MS.gene035320:CDS
TTGCTACCAATAGCAAATGT+TGG	0.481402	8.3:+7418670	MS.gene035320:CDS
TGCTACCAATAGCAAATGTT+GGG	0.486912	8.3:+7418671	MS.gene035320:CDS
GAAAGGAGAAAGACAGTGAA+TGG	0.488041	8.3:+7418820	MS.gene035320:CDS
ATGATATCTGTTGGGCTTTG+GGG	0.489965	8.3:+7418845	MS.gene035320:CDS
GTGAGGCATCAGAGAAATGC+AGG	0.490175	8.3:+7418794	MS.gene035320:CDS
TCCTTTAGTAATGATGAAAA+CGG	0.512602	8.3:+7418622	MS.gene035320:CDS
CAGAGAAATGCAGGAAGGAA+AGG	0.518431	8.3:+7418803	MS.gene035320:CDS
GAATGGTGATGATATCTGTT+GGG	0.569420	8.3:+7418837	MS.gene035320:CDS
TATAAGCTTTGTGACAAGTG+AGG	0.602260	8.3:+7418777	MS.gene035320:CDS
ATGATGGATAACAACGTTGG+AGG	0.607529	8.3:+7418595	MS.gene035320:CDS
TGAATGGTGATGATATCTGT+TGG	0.622578	8.3:+7418836	MS.gene035320:CDS
GAAATGATGGATAACAACGT+TGG	0.629287	8.3:+7418592	None:intergenic
TTTAGTAATGATGAAAACGG+AGG	0.646824	8.3:+7418625	MS.gene035320:CDS
ATTACGCAGAACCAATGAGA+AGG	0.655098	8.3:+7418884	MS.gene035320:CDS
TACCAATAGCAAATGTTGGG+AGG	0.664077	8.3:+7418674	MS.gene035320:CDS
GGCATCAGAGAAATGCAGGA+AGG	0.689891	8.3:+7418798	MS.gene035320:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAATCTCAAAGGAATCAA+AGG	+	chr8.3:7418726-7418745	MS.gene035320:CDS	25.0%
!	TCCTTTAGTAATGATGAAAA+CGG	+	chr8.3:7418622-7418641	MS.gene035320:CDS	25.0%
	ACAGAATGCAAAAATCTCAA+AGG	+	chr8.3:7418717-7418736	MS.gene035320:CDS	30.0%
	GCATAATAAAGGAACAAGAT+AGG	+	chr8.3:7418647-7418666	MS.gene035320:CDS	30.0%
	GCATAGATATAGAGAATTAG+AGG	+	chr8.3:7418912-7418931	MS.gene035320:CDS	30.0%
	GCTAATAATATTCCAGATAG+AGG	+	chr8.3:7418955-7418974	MS.gene035320:CDS	30.0%
	GGAATATTATTAGCAGTAGT+TGG	-	chr8.3:7418949-7418968	None:intergenic	30.0%
	TTTAGTAATGATGAAAACGG+AGG	+	chr8.3:7418625-7418644	MS.gene035320:CDS	30.0%
!	TCCGTTTTCATCATTACTAA+AGG	-	chr8.3:7418626-7418645	None:intergenic	30.0%
!!	TTTGAGATTTTTGCATTCTG+TGG	-	chr8.3:7418718-7418737	None:intergenic	30.0%
	CTATGCAAATACCTTCTCAT+TGG	-	chr8.3:7418898-7418917	None:intergenic	35.0%
	TGCTACCAATAGCAAATGTT+GGG	+	chr8.3:7418671-7418690	MS.gene035320:CDS	35.0%
	TTGCTACCAATAGCAAATGT+TGG	+	chr8.3:7418670-7418689	MS.gene035320:CDS	35.0%
!	GAATGGTGATGATATCTGTT+GGG	+	chr8.3:7418837-7418856	MS.gene035320:CDS	35.0%
!	TATAAGCTTTGTGACAAGTG+AGG	+	chr8.3:7418777-7418796	MS.gene035320:CDS	35.0%
!	TGAAAACGGAGGCATAATAA+AGG	+	chr8.3:7418636-7418655	MS.gene035320:CDS	35.0%
!	TGAATGGTGATGATATCTGT+TGG	+	chr8.3:7418836-7418855	MS.gene035320:CDS	35.0%
	ATGATATCTGTTGGGCTTTG+GGG	+	chr8.3:7418845-7418864	MS.gene035320:CDS	40.0%
	ATTACGCAGAACCAATGAGA+AGG	+	chr8.3:7418884-7418903	MS.gene035320:CDS	40.0%
	GAAAGGAGAAAGACAGTGAA+TGG	+	chr8.3:7418820-7418839	MS.gene035320:CDS	40.0%
	GATGATATCTGTTGGGCTTT+GGG	+	chr8.3:7418844-7418863	MS.gene035320:CDS	40.0%
	TACCAATAGCAAATGTTGGG+AGG	+	chr8.3:7418674-7418693	MS.gene035320:CDS	40.0%
	TGATGATATCTGTTGGGCTT+TGG	+	chr8.3:7418843-7418862	MS.gene035320:CDS	40.0%
!!	ATCCTCCCAACATTTGCTAT+TGG	-	chr8.3:7418679-7418698	None:intergenic	40.0%
!!	ATGATGGATAACAACGTTGG+AGG	+	chr8.3:7418595-7418614	MS.gene035320:CDS	40.0%
	CAGAGAAATGCAGGAAGGAA+AGG	+	chr8.3:7418803-7418822	MS.gene035320:CDS	45.0%
	GGCATCAGAGAAATGCAGGA+AGG	+	chr8.3:7418798-7418817	MS.gene035320:CDS	50.0%
!	GTGAGGCATCAGAGAAATGC+AGG	+	chr8.3:7418794-7418813	MS.gene035320:CDS	50.0%
!!	TGTTGGGCTTTGGGGACACT+TGG	+	chr8.3:7418853-7418872	MS.gene035320:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	7418595	7418984	7418595	ID=MS.gene035320
chr8.3	mRNA	7418595	7418984	7418595	ID=MS.gene035320.t1;Parent=MS.gene035320
chr8.3	exon	7418595	7418984	7418595	ID=MS.gene035320.t1.exon1;Parent=MS.gene035320.t1
chr8.3	CDS	7418595	7418984	7418595	ID=cds.MS.gene035320.t1;Parent=MS.gene035320.t1

Gene Sequence

>MS.gene035320

ATGATGGATAACAACGTTGGAGGAAGTTCCTTTAGTAATGATGAAAACGGAGGCATAATAAAGGAACAAGATAGGTTGCTACCAATAGCAAATGTTGGGAGGATAATGAAGCAGATATTACCACAGAATGCAAAAATCTCAAAGGAATCAAAGGAAACAATGCAAGAGTGTGTTTCAGAGTTTATAAGCTTTGTGACAAGTGAGGCATCAGAGAAATGCAGGAAGGAAAGGAGAAAGACAGTGAATGGTGATGATATCTGTTGGGCTTTGGGGACACTTGGTTTTGATGATTACGCAGAACCAATGAGAAGGTATTTGCATAGATATAGAGAATTAGAGGTAGATAGAACACCAACTACTGCTAATAATATTCCAGATAGAGGAGATTAA

Protein sequence

>MS.gene035320.t1

MMDNNVGGSSFSNDENGGIIKEQDRLLPIANVGRIMKQILPQNAKISKESKETMQECVSEFISFVTSEASEKCRKERRKTVNGDDICWALGTLGFDDYAEPMRRYLHRYRELEVDRTPTTANNIPDRGD