AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035388

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035388.t1 MTR_4g120380 65.909 88 30 0 2 89 243 330 1.32e-39 134
MS.gene035388.t1 MTR_4g120380 65.909 88 30 0 2 89 243 330 7.06e-39 133
MS.gene035388.t1 MTR_4g120380 81.356 59 11 0 31 89 351 409 1.23e-28 106
MS.gene035388.t1 MTR_4g119580 76.136 88 21 0 2 89 243 330 5.00e-36 125
MS.gene035388.t1 MTR_2g437020 55.056 89 40 0 1 89 241 329 1.55e-30 110
MS.gene035388.t1 MTR_2g437040 54.023 87 40 0 3 89 242 328 2.19e-29 107
MS.gene035388.t1 MTR_2g437030 56.818 88 38 0 2 89 327 414 3.29e-29 108
MS.gene035388.t1 MTR_2g436460 53.409 88 41 0 2 89 247 334 5.76e-29 106
MS.gene035388.t1 MTR_2g437200 54.545 88 40 0 2 89 269 356 6.84e-29 106
MS.gene035388.t1 MTR_2g437990 53.409 88 41 0 2 89 253 340 1.53e-28 106
MS.gene035388.t1 MTR_2g076320 52.273 88 42 0 2 89 254 341 2.57e-28 105
MS.gene035388.t1 MTR_2g437080 54.545 88 40 0 2 89 241 328 2.95e-28 104
MS.gene035388.t1 MTR_2g438010 56.098 82 36 0 8 89 280 361 3.43e-28 105
MS.gene035388.t1 MTR_2g436400 54.023 87 40 0 3 89 249 335 4.33e-28 104
MS.gene035388.t1 MTR_2g437090 53.409 88 41 0 2 89 29 116 5.45e-28 99.8
MS.gene035388.t1 MTR_2g437060 51.136 88 43 0 2 89 256 343 5.50e-28 104
MS.gene035388.t1 MTR_2g437940 53.409 88 41 0 2 89 225 312 8.85e-28 103
MS.gene035388.t1 MTR_2g437180 51.136 88 43 0 2 89 253 340 1.16e-26 100
MS.gene035388.t1 MTR_2g437960 50.000 88 44 0 2 89 257 344 2.02e-26 100
MS.gene035388.t1 MTR_2g437960 50.000 88 44 0 2 89 257 344 2.12e-26 100
MS.gene035388.t1 MTR_2g437150 50.000 88 44 0 2 89 261 348 7.62e-26 98.6
MS.gene035388.t1 MTR_2g437130 47.727 88 46 0 2 89 242 329 9.66e-26 98.2
MS.gene035388.t1 MTR_2g436440 51.136 88 43 0 2 89 247 334 2.48e-25 97.1
MS.gene035388.t1 MTR_2g437240 50.588 85 42 0 5 89 272 356 2.80e-25 97.1
MS.gene035388.t1 MTR_2g437170 48.864 88 45 0 2 89 261 348 5.60e-25 96.3
MS.gene035388.t1 MTR_2g437260 46.067 89 48 0 1 89 236 324 8.17e-25 95.5
MS.gene035388.t1 MTR_2g438020 51.136 88 43 0 2 89 308 395 9.42e-25 96.3
MS.gene035388.t1 MTR_2g437120 48.837 86 44 0 4 89 256 341 2.12e-24 94.7
MS.gene035388.t1 MTR_2g437160 48.864 88 45 0 2 89 175 262 2.16e-23 90.9
MS.gene035388.t1 MTR_2g437160 48.864 88 45 0 2 89 266 353 4.62e-23 90.9
MS.gene035388.t1 MTR_2g437100 46.591 88 47 0 2 89 266 353 7.38e-23 90.5
MS.gene035388.t1 MTR_4g119550 46.591 88 47 0 2 89 251 338 1.63e-22 89.4
MS.gene035388.t1 MTR_2g019810 46.512 86 46 0 4 89 233 318 1.68e-19 80.9
MS.gene035388.t1 MTR_2g019840 45.122 82 45 0 8 89 249 330 3.58e-19 80.1
MS.gene035388.t1 MTR_4g119570 46.591 88 14 1 2 89 135 189 1.45e-18 76.6
MS.gene035388.t1 MTR_3g450500 33.721 86 57 0 3 88 244 329 1.42e-12 61.6
MS.gene035388.t1 MTR_3g085240 45.614 57 31 0 33 89 176 232 9.65e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035388.t1 AT5G07900 40.000 85 51 0 4 88 275 359 1.31e-17 75.9
MS.gene035388.t1 AT5G64950 36.471 85 54 0 2 86 244 328 6.00e-13 62.8

Find 0 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATACTAAATCCGAAAATATC+TGG - 32542:18695-18714 MS.gene035388:intergenic 25.0%
TCGGATTTAGTATAGAGAAA+AGG + 32542:18702-18721 MS.gene035388:CDS 30.0%
! TTGAAAGCTCCAGATATTTT+CGG + 32542:18683-18702 MS.gene035388:CDS 30.0%
AGAGAAAAGGCTTATTCCAA+GGG + 32542:18715-18734 MS.gene035388:CDS 35.0%
TAGAGAAAAGGCTTATTCCA+AGG + 32542:18714-18733 MS.gene035388:CDS 35.0%
TTTGATCCAAAAACGCATCA+CGG - 32542:18636-18655 MS.gene035388:intergenic 35.0%
! ATCAACTTTTGCATCCCAAT+GGG - 32542:18519-18538 MS.gene035388:intergenic 35.0%
TTGGATCAAACAGCATGGTT+GGG + 32542:18646-18665 MS.gene035388:CDS 40.0%
TTTGGATCAAACAGCATGGT+TGG + 32542:18645-18664 MS.gene035388:CDS 40.0%
! AGTTGATGCCTTCAAGAGTT+GGG + 32542:18532-18551 MS.gene035388:CDS 40.0%
! CATCAACTTTTGCATCCCAA+TGG - 32542:18520-18539 MS.gene035388:intergenic 40.0%
! TTTTGCATCCCAATGGGATT+TGG - 32542:18513-18532 MS.gene035388:intergenic 40.0%
!! AAGTTGATGCCTTCAAGAGT+TGG + 32542:18531-18550 MS.gene035388:CDS 40.0%
!! CGTTTTTGGATCAAACAGCA+TGG + 32542:18641-18660 MS.gene035388:CDS 40.0%
!! GAAAGCCTAGCTTTATGCTA+CGG + 32542:18591-18610 MS.gene035388:CDS 40.0%
!! TAGCTTTATGCTACGGTCTA+GGG + 32542:18598-18617 MS.gene035388:CDS 40.0%
AGAACAACCCCAACTCTTGA+AGG - 32542:18543-18562 MS.gene035387:intergenic 45.0%
CTAGACCGTAGCATAAAGCT+AGG - 32542:18599-18618 MS.gene035388:intergenic 45.0%
GGCTATCTCCAAATCCCATT+GGG + 32542:18502-18521 MS.gene035388:CDS 45.0%
GTTGATGCCTTCAAGAGTTG+GGG + 32542:18533-18552 MS.gene035388:CDS 45.0%
TGGCTATCTCCAAATCCCAT+TGG + 32542:18501-18520 MS.gene035388:CDS 45.0%
! TCAATGCCGTGATGCGTTTT+TGG + 32542:18627-18646 MS.gene035388:CDS 45.0%
!! CTAGCTTTATGCTACGGTCT+AGG + 32542:18597-18616 MS.gene035388:CDS 45.0%
TACTGGACAACAGAAGCCCT+TGG - 32542:18734-18753 MS.gene035388:intergenic 50.0%
! GGAGCTTTCAAGAGCACGAA+AGG - 32542:18674-18693 MS.gene035388:intergenic 50.0%


Chromosome Type Strat End Strand Name
32542 gene 18500 18767 18500 ID=MS.gene035388
32542 mRNA 18500 18767 18500 ID=MS.gene035388.t1;Parent=MS.gene035388
32542 exon 18500 18767 18500 ID=MS.gene035388.t1.exon1;Parent=MS.gene035388.t1
32542 CDS 18500 18767 18500 ID=cds.MS.gene035388.t1;Parent=MS.gene035388.t1
Gene Sequence

>MS.gene035388

ATGGCTATCTCCAAATCCCATTGGGATGCAAAAGTTGATGCCTTCAAGAGTTGGGGTTGTTCTGAAGATGATATTTTGACTGCATTTAAAAGAAAGCCTAGCTTTATGCTACGGTCTAGGGACAAACTCAATGCCGTGATGCGTTTTTGGATCAAACAGCATGGTTGGGATCCTTTCGTGCTCTTGAAAGCTCCAGATATTTTCGGATTTAGTATAGAGAAAAGGCTTATTCCAAGGGCTTCTGTTGTCCAGTATCTTCTTTCCAAAG
Protein sequence

>MS.gene035388.t1

MAISKSHWDAKVDAFKSWGCSEDDILTAFKRKPSFMLRSRDKLNAVMRFWIKQHGWDPFVLLKAPDIFGFSIEKRLIPRASVVQYLLSK