AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035421

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035421.t1 MTR_8g442410 95.896 536 22 0 6 541 1 536 0.0 1053
MS.gene035421.t1 MTR_4g104020 46.649 388 186 8 154 530 143 520 6.55e-103 320
MS.gene035421.t1 MTR_7g027190 41.604 399 196 6 152 530 292 673 2.30e-93 299
MS.gene035421.t1 MTR_3g065980 35.606 396 217 10 154 531 166 541 6.40e-77 253
MS.gene035421.t1 MTR_3g089055 34.921 378 227 6 161 530 199 565 3.17e-66 225
MS.gene035421.t1 MTR_3g089055 34.921 378 227 6 161 530 204 570 3.24e-66 225
MS.gene035421.t1 MTR_5g094450 33.592 387 234 7 156 530 157 532 2.06e-64 219
MS.gene035421.t1 MTR_1g029420 34.037 379 229 7 161 530 226 592 7.87e-64 219
MS.gene035421.t1 MTR_5g097480 33.594 384 234 8 156 530 173 544 8.39e-64 218
MS.gene035421.t1 MTR_2g082090 36.061 391 215 9 156 530 208 579 1.41e-63 218
MS.gene035421.t1 MTR_3g056110 32.552 384 238 7 156 530 171 542 4.32e-63 216
MS.gene035421.t1 MTR_4g064150 30.467 407 257 7 140 532 341 735 2.74e-58 207
MS.gene035421.t1 MTR_7g074650 35.501 369 218 8 164 530 442 792 8.74e-58 206
MS.gene035421.t1 MTR_4g102790 32.990 388 240 11 152 529 284 661 1.06e-57 204
MS.gene035421.t1 MTR_4g064160 30.827 399 251 8 148 532 299 686 3.12e-56 200
MS.gene035421.t1 MTR_4g133660 32.041 387 237 7 155 529 170 542 3.35e-56 197
MS.gene035421.t1 MTR_4g064120 32.383 386 242 9 152 527 246 622 6.71e-56 198
MS.gene035421.t1 MTR_4g064180 31.510 384 248 6 152 527 246 622 1.01e-55 198
MS.gene035421.t1 MTR_2g097410 29.360 453 292 7 85 529 308 740 6.95e-54 194
MS.gene035421.t1 MTR_4g064200 30.990 384 243 7 155 529 279 649 7.23e-53 190
MS.gene035421.t1 MTR_4g076020 35.000 380 213 11 164 538 88 438 1.05e-52 185
MS.gene035421.t1 MTR_2g097467 29.688 384 249 6 154 529 283 653 6.91e-52 187
MS.gene035421.t1 MTR_2g097310 28.916 415 256 11 134 532 249 640 2.19e-51 186
MS.gene035421.t1 MTR_7g062120 29.899 398 259 6 144 532 356 742 9.28e-51 186
MS.gene035421.t1 MTR_2g097463 29.793 386 250 6 155 532 285 657 1.51e-50 184
MS.gene035421.t1 MTR_2g097473 28.941 387 254 6 154 532 283 656 1.66e-49 181
MS.gene035421.t1 MTR_1g069725 33.590 390 219 11 152 529 101 462 1.78e-49 177
MS.gene035421.t1 MTR_2g097390 29.949 394 234 10 155 529 270 640 1.90e-49 181
MS.gene035421.t1 MTR_2g097350 30.127 395 232 12 155 529 269 639 7.39e-49 179
MS.gene035421.t1 MTR_2g026250 29.091 385 253 7 155 530 223 596 4.57e-47 173
MS.gene035421.t1 MTR_2g034280 31.606 386 250 9 153 530 196 575 4.30e-45 167
MS.gene035421.t1 MTR_2g034250 30.848 389 252 10 153 530 203 585 1.22e-44 166
MS.gene035421.t1 MTR_3g022830 28.738 428 230 11 161 530 26 436 1.95e-44 163
MS.gene035421.t1 MTR_2g097380 28.686 373 217 9 165 529 229 560 2.99e-44 165
MS.gene035421.t1 MTR_3g072710 32.840 405 228 15 152 529 112 499 1.08e-43 162
MS.gene035421.t1 MTR_5g058860 29.240 513 303 18 44 529 17 496 1.66e-42 159
MS.gene035421.t1 MTR_5g015490 28.272 382 256 7 161 530 115 490 1.30e-41 156
MS.gene035421.t1 MTR_4g097080 28.684 380 251 11 161 530 134 503 2.33e-41 156
MS.gene035421.t1 MTR_2g034260 30.075 399 241 11 153 530 203 584 5.79e-41 156
MS.gene035421.t1 MTR_7g057230 31.563 339 207 9 200 529 334 656 3.32e-39 152
MS.gene035421.t1 MTR_1g096030 29.369 412 230 17 154 530 74 459 6.91e-38 145
MS.gene035421.t1 MTR_4g076140 45.270 148 79 1 385 530 322 469 1.75e-37 144
MS.gene035421.t1 MTR_4g076140 34.222 225 126 7 153 372 41 248 4.12e-27 114
MS.gene035421.t1 MTR_1g086970 28.087 413 257 13 139 530 85 478 2.49e-37 144
MS.gene035421.t1 MTR_5g009080 43.421 152 84 1 382 531 328 479 1.03e-36 142
MS.gene035421.t1 MTR_5g009080 33.333 225 128 7 153 372 49 256 3.82e-27 115
MS.gene035421.t1 MTR_4g077760 28.831 385 244 10 157 530 190 555 2.29e-34 137
MS.gene035421.t1 MTR_6g047750 30.591 389 233 14 159 530 294 662 6.37e-33 133
MS.gene035421.t1 MTR_4g095500 27.163 416 248 12 161 529 60 467 1.17e-32 130
MS.gene035421.t1 MTR_2g099110 28.462 390 246 10 161 530 75 451 4.15e-32 129
MS.gene035421.t1 MTR_2g089100 27.685 419 254 9 146 529 38 442 7.70e-32 128
MS.gene035421.t1 MTR_5g015950 27.098 417 249 15 161 529 36 445 1.23e-31 127
MS.gene035421.t1 MTR_3g053270 27.098 417 249 15 161 529 36 445 1.23e-31 127
MS.gene035421.t1 MTR_7g109580 26.031 388 264 12 152 530 181 554 4.33e-28 118
MS.gene035421.t1 MTR_8g093070 25.628 398 255 14 157 540 136 506 2.61e-25 109
MS.gene035421.t1 MTR_0092s0100 24.352 386 254 10 157 530 372 731 4.88e-22 100
MS.gene035421.t1 MTR_0092s0100 24.352 386 254 10 157 530 369 728 5.41e-22 100
MS.gene035421.t1 MTR_4g026485 22.922 397 263 12 147 530 258 624 5.44e-18 87.8
MS.gene035421.t1 MTR_8g077940 23.514 387 261 14 156 530 178 541 6.38e-16 81.3
MS.gene035421.t1 MTR_8g020840 22.362 398 262 13 161 531 172 549 1.23e-15 80.1
MS.gene035421.t1 MTR_3g022580 35.385 130 74 4 409 530 56 183 1.49e-15 75.5
MS.gene035421.t1 MTR_3g022005 37.634 93 55 2 440 530 34 125 1.02e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035421.t1 AT1G66350 37.598 383 210 11 154 531 149 507 2.78e-76 250
MS.gene035421.t1 AT3G03450 36.719 384 225 8 154 532 177 547 1.41e-74 246
MS.gene035421.t1 AT1G14920 37.143 385 217 10 154 532 166 531 1.35e-73 243
MS.gene035421.t1 AT2G01570 37.150 393 214 13 154 536 218 587 3.35e-72 241
MS.gene035421.t1 AT5G17490 32.252 493 283 15 69 532 48 518 2.40e-67 227
MS.gene035421.t1 AT2G04890 34.115 384 225 7 156 530 49 413 1.60e-63 213
MS.gene035421.t1 AT5G48150 33.420 383 237 6 156 530 118 490 6.28e-62 211
MS.gene035421.t1 AT5G48150 33.420 383 237 6 156 530 118 490 6.28e-62 211
MS.gene035421.t1 AT5G48150 33.420 383 237 6 156 530 118 490 6.28e-62 211
MS.gene035421.t1 AT5G48150 33.420 383 237 6 156 530 118 490 6.28e-62 211
MS.gene035421.t1 AT3G54220 37.197 371 210 8 164 529 297 649 1.82e-61 214
MS.gene035421.t1 AT1G21450 29.828 466 302 7 76 530 142 593 8.20e-60 208
MS.gene035421.t1 AT1G50600 32.552 384 238 7 156 530 155 526 4.90e-58 202
MS.gene035421.t1 AT5G41920 34.737 380 229 9 154 530 37 400 8.13e-58 198
MS.gene035421.t1 AT1G50600 32.552 384 238 7 156 530 226 597 2.98e-57 201
MS.gene035421.t1 AT1G50600 32.552 384 238 7 156 530 230 601 3.50e-57 201
MS.gene035421.t1 AT1G07530 28.605 430 285 8 110 529 347 764 2.35e-56 202
MS.gene035421.t1 AT2G29060 30.846 402 255 7 140 529 264 654 3.71e-53 191
MS.gene035421.t1 AT4G17230 32.995 394 237 8 149 530 147 525 8.72e-53 188
MS.gene035421.t1 AT4G17230 32.995 394 237 8 149 530 147 525 8.72e-53 188
MS.gene035421.t1 AT2G29060 30.846 402 255 7 140 529 301 691 1.06e-52 191
MS.gene035421.t1 AT2G29065 27.216 485 320 12 65 529 156 627 1.57e-51 186
MS.gene035421.t1 AT1G07520 26.856 458 293 9 85 529 100 528 5.70e-50 180
MS.gene035421.t1 AT1G07520 26.856 458 293 9 85 529 100 528 5.70e-50 180
MS.gene035421.t1 AT1G07520 26.856 458 293 9 85 529 264 692 3.09e-49 181
MS.gene035421.t1 AT1G07520 26.856 458 293 9 85 529 265 693 3.35e-49 181
MS.gene035421.t1 AT5G59450 28.406 433 283 10 111 532 184 600 5.82e-49 179
MS.gene035421.t1 AT1G55580 32.447 376 217 10 189 530 73 445 2.71e-48 174
MS.gene035421.t1 AT1G63100 31.006 358 231 7 151 503 243 589 7.05e-48 176
MS.gene035421.t1 AT1G63100 31.006 358 231 7 151 503 268 614 8.72e-48 176
MS.gene035421.t1 AT3G46600 29.188 394 252 8 152 533 204 582 2.24e-47 174
MS.gene035421.t1 AT3G46600 29.188 394 252 8 152 533 172 550 5.14e-47 172
MS.gene035421.t1 AT3G46600 29.351 385 245 8 161 533 5 374 6.14e-47 168
MS.gene035421.t1 AT2G37650 28.278 389 259 6 150 530 325 701 5.85e-46 172
MS.gene035421.t1 AT2G37650 28.278 389 259 6 150 530 337 713 6.43e-46 172
MS.gene035421.t1 AT4G37650 27.805 410 270 12 141 530 126 529 7.27e-43 160
MS.gene035421.t1 AT5G66770 29.793 386 241 10 157 530 217 584 3.21e-39 151
MS.gene035421.t1 AT4G08250 29.668 391 249 14 152 529 99 476 1.09e-38 148
MS.gene035421.t1 AT3G50650 30.168 358 217 9 188 530 203 542 2.33e-38 148
MS.gene035421.t1 AT5G52510 31.579 342 197 10 217 530 308 640 1.12e-37 147
MS.gene035421.t1 AT1G50420 43.137 153 85 1 380 530 327 479 6.08e-35 137
MS.gene035421.t1 AT1G50420 32.444 225 138 4 153 375 50 262 4.24e-29 120
MS.gene035421.t1 AT3G49950 27.708 397 239 15 161 529 31 407 2.95e-31 125
MS.gene035421.t1 AT3G60630 26.710 307 201 10 238 530 326 622 7.76e-22 99.8
MS.gene035421.t1 AT4G00150 27.714 350 219 11 186 530 234 554 1.31e-19 92.8
MS.gene035421.t1 AT4G36710 22.750 400 257 13 152 530 117 485 1.89e-18 88.6
MS.gene035421.t1 AT2G45160 24.459 462 287 19 99 530 210 639 1.53e-17 86.7
MS.gene035421.t1 AT3G13840 24.878 410 262 17 141 530 127 510 1.58e-15 79.7

Find 102 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAAATGAACGGCAATTT+TGG 0.207673 8.2:-52037004 MS.gene035421:CDS
CTCAATACACATAATTGATT+TGG 0.225391 8.2:-52036588 MS.gene035421:CDS
CACAATGGACCTTTCTTTCT+TGG 0.232793 8.2:-52036185 MS.gene035421:CDS
TTCCTTTCCAACCAAGTAAA+AGG 0.254662 8.2:+52035862 None:intergenic
TTCAATGGCCTTCGCTAATA+AGG 0.263625 8.2:-52036550 MS.gene035421:CDS
TTTGTTACTTGGTAAAATTT+TGG 0.264110 8.2:-52036870 MS.gene035421:CDS
GCCGGGCAAAACCTTCTAAT+AGG 0.275207 8.2:+52037340 None:intergenic
ATCCTGATTGGACTATTTCT+TGG 0.280256 8.2:+52036101 None:intergenic
TGATCTTGTTCCATTCTTGC+CGG 0.294596 8.2:+52037322 None:intergenic
ACAACACCTTATATTACTTT+TGG 0.300835 8.2:-52036659 MS.gene035421:CDS
GATCAATGGAGAAGACAATT+AGG 0.324260 8.2:-52035990 MS.gene035421:CDS
TCATGTCTCTCCATTCTATC+AGG 0.340101 8.2:+52036020 None:intergenic
ATGATGTTTCTTTGATTACT+AGG 0.342089 8.2:-52036718 MS.gene035421:CDS
GACACTTTCATTCATATAGA+AGG 0.344185 8.2:+52037375 None:intergenic
TTGGCTTTGTCAAGTCCTTC+AGG 0.354507 8.2:-52036851 MS.gene035421:CDS
ATGAAACCAAAAGTAATATA+AGG 0.362911 8.2:+52036653 None:intergenic
CCTCCAAAGCTCAGGATAAC+AGG 0.367635 8.2:-52036503 MS.gene035421:CDS
CCTGTTATCCTGAGCTTTGG+AGG 0.384703 8.2:+52036503 None:intergenic
ACCTTGATTTCCCTATTAGA+AGG 0.391553 8.2:-52037351 MS.gene035421:CDS
CTGAGCTTTGGAGGCCCCTC+AGG 0.392034 8.2:+52036512 None:intergenic
TCAATACACATAATTGATTT+GGG 0.393109 8.2:-52036587 MS.gene035421:CDS
TTGGTGAATGATGATGTCTT+TGG 0.396171 8.2:-52037103 MS.gene035421:CDS
TGACTTGGTCCATACGCTAC+TGG 0.405114 8.2:-52036936 MS.gene035421:CDS
ATATACTTTGTCATGTGAAA+AGG 0.405685 8.2:-52035889 MS.gene035421:CDS
CCGGGCAAAACCTTCTAATA+GGG 0.412886 8.2:+52037341 None:intergenic
TCGACAACTGTGAGTGCAAT+TGG 0.432243 8.2:+52036221 None:intergenic
AAGTAATATAAGGTGTTGTT+TGG 0.439313 8.2:+52036663 None:intergenic
AATCCTGTTATCCTGAGCTT+TGG 0.439914 8.2:+52036500 None:intergenic
AATCAGGCGAATTTGTTACT+TGG 0.447598 8.2:-52036881 MS.gene035421:CDS
GATCTTGTTCCATTCTTGCC+GGG 0.448030 8.2:+52037323 None:intergenic
TTGTCGATTGTAAGAAGAGA+AGG 0.450373 8.2:+52036362 None:intergenic
TGTAGAGGAAGCACTCTCAT+TGG 0.457377 8.2:-52036426 MS.gene035421:CDS
GACATGAAAGAGTAGATCAA+TGG 0.459154 8.2:-52036004 MS.gene035421:CDS
ATGGACCTTTCTTTCTTGGT+AGG 0.464231 8.2:-52036181 MS.gene035421:CDS
GCTATATGCCAAGGATCAAA+AGG 0.466005 8.2:-52036617 MS.gene035421:CDS
CTATTTGAAAATACATTATG+TGG 0.466093 8.2:+52036764 None:intergenic
GAAAGGAAGGTCTATTATGA+TGG 0.467802 8.2:-52035847 MS.gene035421:CDS
ACCTTCTAATAGGGAAATCA+AGG 0.468486 8.2:+52037350 None:intergenic
GTAGAGGAAGCACTCTCATT+GGG 0.469254 8.2:-52036425 MS.gene035421:CDS
TGCCAAGAAATAGTCCAATC+AGG 0.469563 8.2:-52036103 MS.gene035421:CDS
CTGGCTTGTGCCGAGGCCGT+TGG 0.479503 8.2:-52036917 MS.gene035421:CDS
TGATGTTTCTTTGATTACTA+GGG 0.480820 8.2:-52036717 MS.gene035421:CDS
TCTTCATCCTCCATTGTGGT+TGG 0.481380 8.2:+52037073 None:intergenic
GCCTTGGTTCATACCGAGAC+TGG 0.481981 8.2:+52036961 None:intergenic
GATTACTAGGGAGAATAAGT+TGG 0.489189 8.2:-52036705 MS.gene035421:CDS
CATCATCAAGAACTGTCTCT+TGG 0.496418 8.2:+52037281 None:intergenic
GAATGGAGCATGCTCTTCAA+TGG 0.496563 8.2:-52036565 MS.gene035421:CDS
TTGCCACAGCATTGAAGTGT+AGG 0.499786 8.2:-52036799 MS.gene035421:CDS
GTCTCTGCAACCAACGGCCT+CGG 0.503923 8.2:+52036907 None:intergenic
AGAAGACAATTAGGAAGAGC+AGG 0.503981 8.2:-52035981 MS.gene035421:CDS
CTGATATTATGCGAAATTCA+AGG 0.506362 8.2:+52036396 None:intergenic
TAATAAGGAACCTCGCTTCG+AGG 0.507019 8.2:-52036535 MS.gene035421:CDS
CCGAGACTGGCTTGTGATTG+TGG 0.515572 8.2:+52036974 None:intergenic
GCCCCTCAGGCCTCGAAGCG+AGG 0.518387 8.2:+52036525 None:intergenic
CTTTCTGTTTATGATTGTGA+TGG 0.518538 8.2:-52035912 MS.gene035421:CDS
CTATTATGATGGCTTCTGCT+TGG 0.519258 8.2:-52035836 MS.gene035421:CDS
TATACTTTGTCATGTGAAAA+GGG 0.523287 8.2:-52035888 MS.gene035421:CDS
CGGCACAAGCCAGTAGCGTA+TGG 0.524974 8.2:+52036927 None:intergenic
CTGAGGGGCCTCCAAAGCTC+AGG 0.525835 8.2:-52036511 MS.gene035421:CDS
AGCAATATTTGATTCTCTTG+AGG 0.528353 8.2:-52036132 MS.gene035421:CDS
TATGGACCAAGTCAAGGCCT+TGG 0.530054 8.2:+52036945 None:intergenic
ACACATAATTGATTTGGGAA+TGG 0.530660 8.2:-52036582 MS.gene035421:CDS
AAGTCATCAACTTCTGAAGA+AGG 0.531009 8.2:+52037214 None:intergenic
AGAAACCTACCAAGAAAGAA+AGG 0.532154 8.2:+52036176 None:intergenic
AACCTCGCTTCGAGGCCTGA+GGG 0.533889 8.2:-52036527 MS.gene035421:CDS
ATTCACCTTCTCTCAAGTTG+AGG 0.536949 8.2:+52036339 None:intergenic
TTGTGAGGGTCCTGATAGAA+TGG 0.538774 8.2:-52036030 MS.gene035421:CDS
TTGCAACATTGTAGCTTGTG+AGG 0.544201 8.2:-52036045 MS.gene035421:CDS
GTAAGAAGAGAAGGAGTTGT+CGG 0.546160 8.2:+52036371 None:intergenic
CAAGAACTGTCTCTTGGTCA+TGG 0.547218 8.2:+52037287 None:intergenic
AAATTCTTCATCCTCCATTG+TGG 0.547730 8.2:+52037069 None:intergenic
TGCAACATTGTAGCTTGTGA+GGG 0.548434 8.2:-52036044 MS.gene035421:CDS
CAATGCTGTGGCAAAGCAGT+AGG 0.548768 8.2:+52036808 None:intergenic
TGTTGATAGCTTCATCAACA+TGG 0.554822 8.2:-52037191 MS.gene035421:CDS
TAGCATGAATTTGCTTGTAG+AGG 0.564272 8.2:-52036441 MS.gene035421:CDS
TACGCTACTGGCTTGTGCCG+AGG 0.566964 8.2:-52036924 MS.gene035421:CDS
GAACCTCGCTTCGAGGCCTG+AGG 0.569227 8.2:-52036528 MS.gene035421:CDS
TGGTTGCAGAGACAACAATC+AGG 0.574071 8.2:-52036897 MS.gene035421:CDS
GACGAAGAAGTTGAAATGAA+CGG 0.577431 8.2:-52037013 MS.gene035421:CDS
GCTTGACTTGTACACTTGAG+TGG 0.583171 8.2:+52035945 None:intergenic
GCGAGGTTCCTTATTAGCGA+AGG 0.586588 8.2:+52036542 None:intergenic
TATGAACCAAGGCCTTGACT+TGG 0.587577 8.2:-52036951 MS.gene035421:CDS
CCACAATCACAAGCCAGTCT+CGG 0.590657 8.2:-52036974 MS.gene035421:CDS
ACCTCGCTTCGAGGCCTGAG+GGG 0.592685 8.2:-52036526 MS.gene035421:CDS
AGGTAATAAACAAGGAGTGG+TGG 0.593777 8.2:+52037234 None:intergenic
GATGGCTTCTGCTTGGCAAG+TGG 0.608972 8.2:-52035829 MS.gene035421:CDS
GACAACCTCAACTTGAGAGA+AGG 0.621509 8.2:-52036344 MS.gene035421:CDS
GCAAATGAAGCTATATGCCA+AGG 0.622841 8.2:-52036626 MS.gene035421:CDS
ATTGTTGTCTCTGCAACCAA+CGG 0.634384 8.2:+52036901 None:intergenic
GCCAGTCTCGGTATGAACCA+AGG 0.638041 8.2:-52036962 MS.gene035421:CDS
GTAGCGTATGGACCAAGTCA+AGG 0.641162 8.2:+52036939 None:intergenic
CTTTGTAACGAATCTCCTGA+AGG 0.642692 8.2:+52036836 None:intergenic
TAACCTACACTTCAATGCTG+TGG 0.647062 8.2:+52036796 None:intergenic
CAATGGAAGACTTAAACATG+TGG 0.648915 8.2:-52037402 MS.gene035421:CDS
AGAAGGTAATAAACAAGGAG+TGG 0.651035 8.2:+52037231 None:intergenic
GAGCAAGACACGAACCACAA+TGG 0.663179 8.2:-52036200 MS.gene035421:CDS
TGGATATGATCCAACCACAA+TGG 0.666133 8.2:-52037083 MS.gene035421:CDS
TCTGAAGAAGGTAATAAACA+AGG 0.670330 8.2:+52037226 None:intergenic
ATATGATCCAACCACAATGG+AGG 0.672820 8.2:-52037080 MS.gene035421:CDS
AAATATGTGAAAGAAAGTAG+AGG 0.675405 8.2:-52036284 MS.gene035421:CDS
CAAGAAAGAAAGGTCCATTG+TGG 0.695513 8.2:+52036186 None:intergenic
AGAATGGAACAAGATCAAGA+AGG 0.697974 8.2:-52037316 MS.gene035421:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAACCAAAAGTAATATA+AGG + chr8.2:52036547-52036566 None:intergenic 20.0%
!! CTATTTGAAAATACATTATG+TGG + chr8.2:52036436-52036455 None:intergenic 20.0%
!!! TCAATACACATAATTGATTT+GGG - chr8.2:52036610-52036629 MS.gene035421:CDS 20.0%
!!! TTATATTACTTTTGGTTTCA+TGG - chr8.2:52036546-52036565 MS.gene035421:CDS 20.0%
!!! TTTGTTACTTGGTAAAATTT+TGG - chr8.2:52036327-52036346 MS.gene035421:CDS 20.0%
! AAATATGTGAAAGAAAGTAG+AGG - chr8.2:52036913-52036932 MS.gene035421:CDS 25.0%
! ATATACTTTGTCATGTGAAA+AGG - chr8.2:52037308-52037327 MS.gene035421:CDS 25.0%
! ATGATGTTTCTTTGATTACT+AGG - chr8.2:52036479-52036498 MS.gene035421:CDS 25.0%
! CTCAATACACATAATTGATT+TGG - chr8.2:52036609-52036628 MS.gene035421:CDS 25.0%
! TATACTTTGTCATGTGAAAA+GGG - chr8.2:52037309-52037328 MS.gene035421:CDS 25.0%
! TGATGTTTCTTTGATTACTA+GGG - chr8.2:52036480-52036499 MS.gene035421:CDS 25.0%
!! ACAACACCTTATATTACTTT+TGG - chr8.2:52036538-52036557 MS.gene035421:CDS 25.0%
!!! AAGTAATATAAGGTGTTGTT+TGG + chr8.2:52036537-52036556 None:intergenic 25.0%
!!! AAGTTTTGATCATCACTTTT+TGG - chr8.2:52036075-52036094 MS.gene035421:CDS 25.0%
AGCAATATTTGATTCTCTTG+AGG - chr8.2:52037065-52037084 MS.gene035421:CDS 30.0%
CTGATATTATGCGAAATTCA+AGG + chr8.2:52036804-52036823 None:intergenic 30.0%
CTTTCTGTTTATGATTGTGA+TGG - chr8.2:52037285-52037304 MS.gene035421:CDS 30.0%
GACACTTTCATTCATATAGA+AGG + chr8.2:52035825-52035844 None:intergenic 30.0%
TCTGAAGAAGGTAATAAACA+AGG + chr8.2:52035974-52035993 None:intergenic 30.0%
!! ACACATAATTGATTTGGGAA+TGG - chr8.2:52036615-52036634 MS.gene035421:CDS 30.0%
AAATTCTTCATCCTCCATTG+TGG + chr8.2:52036131-52036150 None:intergenic 35.0%
ACCTTCTAATAGGGAAATCA+AGG + chr8.2:52035850-52035869 None:intergenic 35.0%
ACCTTGATTTCCCTATTAGA+AGG - chr8.2:52035846-52035865 MS.gene035421:CDS 35.0%
AGAAACCTACCAAGAAAGAA+AGG + chr8.2:52037024-52037043 None:intergenic 35.0%
AGAAGGTAATAAACAAGGAG+TGG + chr8.2:52035969-52035988 None:intergenic 35.0%
AGAATGGAACAAGATCAAGA+AGG - chr8.2:52035881-52035900 MS.gene035421:CDS 35.0%
GAAAGGAAGGTCTATTATGA+TGG - chr8.2:52037350-52037369 MS.gene035421:CDS 35.0%
GACATGAAAGAGTAGATCAA+TGG - chr8.2:52037193-52037212 MS.gene035421:CDS 35.0%
GACGAAGAAGTTGAAATGAA+CGG - chr8.2:52036184-52036203 MS.gene035421:CDS 35.0%
GATCAATGGAGAAGACAATT+AGG - chr8.2:52037207-52037226 MS.gene035421:CDS 35.0%
GATTACTAGGGAGAATAAGT+TGG - chr8.2:52036492-52036511 MS.gene035421:CDS 35.0%
TTCCTTTCCAACCAAGTAAA+AGG + chr8.2:52037338-52037357 None:intergenic 35.0%
! AAGTCATCAACTTCTGAAGA+AGG + chr8.2:52035986-52036005 None:intergenic 35.0%
! AGCATTTTCCTTTTGATCCT+TGG + chr8.2:52036591-52036610 None:intergenic 35.0%
! ATCCTGATTGGACTATTTCT+TGG + chr8.2:52037099-52037118 None:intergenic 35.0%
! CTCTCTATTTTCATCCTGAT+TGG + chr8.2:52037111-52037130 None:intergenic 35.0%
! GTTGAAATGAACGGCAATTT+TGG - chr8.2:52036193-52036212 MS.gene035421:CDS 35.0%
! TAGCATGAATTTGCTTGTAG+AGG - chr8.2:52036756-52036775 MS.gene035421:CDS 35.0%
! TTGGTGAATGATGATGTCTT+TGG - chr8.2:52036094-52036113 MS.gene035421:CDS 35.0%
! TTGTCGATTGTAAGAAGAGA+AGG + chr8.2:52036838-52036857 None:intergenic 35.0%
! TTTTACTTGGTTGGAAAGGA+AGG - chr8.2:52037337-52037356 MS.gene035421:CDS 35.0%
!! AATCAGGCGAATTTGTTACT+TGG - chr8.2:52036316-52036335 MS.gene035421:CDS 35.0%
!! TGTTGATAGCTTCATCAACA+TGG - chr8.2:52036006-52036025 MS.gene035421:CDS 35.0%
AATCCTGTTATCCTGAGCTT+TGG + chr8.2:52036700-52036719 None:intergenic 40.0%
AGAAGACAATTAGGAAGAGC+AGG - chr8.2:52037216-52037235 MS.gene035421:CDS 40.0%
AGGTAATAAACAAGGAGTGG+TGG + chr8.2:52035966-52035985 None:intergenic 40.0%
ATATGATCCAACCACAATGG+AGG - chr8.2:52036117-52036136 MS.gene035421:CDS 40.0%
ATGGACCTTTCTTTCTTGGT+AGG - chr8.2:52037016-52037035 MS.gene035421:CDS 40.0%
ATTGTTGTCTCTGCAACCAA+CGG + chr8.2:52036299-52036318 None:intergenic 40.0%
CAAGAAAGAAAGGTCCATTG+TGG + chr8.2:52037014-52037033 None:intergenic 40.0%
CACAATGGACCTTTCTTTCT+TGG - chr8.2:52037012-52037031 MS.gene035421:CDS 40.0%
CATCATCAAGAACTGTCTCT+TGG + chr8.2:52035919-52035938 None:intergenic 40.0%
CTATTATGATGGCTTCTGCT+TGG - chr8.2:52037361-52037380 MS.gene035421:CDS 40.0%
CTTTGTAACGAATCTCCTGA+AGG + chr8.2:52036364-52036383 None:intergenic 40.0%
GCAAATGAAGCTATATGCCA+AGG - chr8.2:52036571-52036590 MS.gene035421:CDS 40.0%
GCTATATGCCAAGGATCAAA+AGG - chr8.2:52036580-52036599 MS.gene035421:CDS 40.0%
GTAAGAAGAGAAGGAGTTGT+CGG + chr8.2:52036829-52036848 None:intergenic 40.0%
TAACCTACACTTCAATGCTG+TGG + chr8.2:52036404-52036423 None:intergenic 40.0%
TCATGTCTCTCCATTCTATC+AGG + chr8.2:52037180-52037199 None:intergenic 40.0%
TGATCTTGTTCCATTCTTGC+CGG + chr8.2:52035878-52035897 None:intergenic 40.0%
TGCCAAGAAATAGTCCAATC+AGG - chr8.2:52037094-52037113 MS.gene035421:CDS 40.0%
TGGATATGATCCAACCACAA+TGG - chr8.2:52036114-52036133 MS.gene035421:CDS 40.0%
TTCAATGGCCTTCGCTAATA+AGG - chr8.2:52036647-52036666 MS.gene035421:CDS 40.0%
! ATTCACCTTCTCTCAAGTTG+AGG + chr8.2:52036861-52036880 None:intergenic 40.0%
! GAAAAGGGTTGCCTTTTACT+TGG - chr8.2:52037324-52037343 MS.gene035421:CDS 40.0%
! TGCAACATTGTAGCTTGTGA+GGG - chr8.2:52037153-52037172 MS.gene035421:CDS 40.0%
! TGCCTTTTACTTGGTTGGAA+AGG - chr8.2:52037333-52037352 MS.gene035421:CDS 40.0%
! TTGCAACATTGTAGCTTGTG+AGG - chr8.2:52037152-52037171 MS.gene035421:CDS 40.0%
CAAGAACTGTCTCTTGGTCA+TGG + chr8.2:52035913-52035932 None:intergenic 45.0%
CCGGGCAAAACCTTCTAATA+GGG + chr8.2:52035859-52035878 None:intergenic 45.0%
GAATGGAGCATGCTCTTCAA+TGG - chr8.2:52036632-52036651 MS.gene035421:CDS 45.0%
GACAACCTCAACTTGAGAGA+AGG - chr8.2:52036853-52036872 MS.gene035421:CDS 45.0%
GATCTTGTTCCATTCTTGCC+GGG + chr8.2:52035877-52035896 None:intergenic 45.0%
TAATAAGGAACCTCGCTTCG+AGG - chr8.2:52036662-52036681 MS.gene035421:CDS 45.0%
TATGAACCAAGGCCTTGACT+TGG - chr8.2:52036246-52036265 MS.gene035421:CDS 45.0%
TCGACAACTGTGAGTGCAAT+TGG + chr8.2:52036979-52036998 None:intergenic 45.0%
TCTTCATCCTCCATTGTGGT+TGG + chr8.2:52036127-52036146 None:intergenic 45.0%
TGGTTGCAGAGACAACAATC+AGG - chr8.2:52036300-52036319 MS.gene035421:CDS 45.0%
TTGTGAGGGTCCTGATAGAA+TGG - chr8.2:52037167-52037186 MS.gene035421:CDS 45.0%
! AGGGTTGCCTTTTACTTGGT+TGG - chr8.2:52037328-52037347 MS.gene035421:CDS 45.0%
! CCCTATTAGAAGGTTTTGCC+CGG - chr8.2:52035856-52035875 MS.gene035421:CDS 45.0%
! TTGCCACAGCATTGAAGTGT+AGG - chr8.2:52036398-52036417 MS.gene035421:CDS 45.0%
!! GCTTGACTTGTACACTTGAG+TGG + chr8.2:52037255-52037274 None:intergenic 45.0%
!! GTAGAGGAAGCACTCTCATT+GGG - chr8.2:52036772-52036791 MS.gene035421:CDS 45.0%
!! TGTAGAGGAAGCACTCTCAT+TGG - chr8.2:52036771-52036790 MS.gene035421:CDS 45.0%
!! TTGGCTTTGTCAAGTCCTTC+AGG - chr8.2:52036346-52036365 MS.gene035421:CDS 45.0%
CAATGCTGTGGCAAAGCAGT+AGG + chr8.2:52036392-52036411 None:intergenic 50.0%
CCACAATCACAAGCCAGTCT+CGG - chr8.2:52036223-52036242 MS.gene035421:CDS 50.0%
CCTCCAAAGCTCAGGATAAC+AGG - chr8.2:52036694-52036713 MS.gene035421:CDS 50.0%
GAGCAAGACACGAACCACAA+TGG - chr8.2:52036997-52037016 MS.gene035421:CDS 50.0%
GCCGGGCAAAACCTTCTAAT+AGG + chr8.2:52035860-52035879 None:intergenic 50.0%
GCGAGGTTCCTTATTAGCGA+AGG + chr8.2:52036658-52036677 None:intergenic 50.0%
GTAGCGTATGGACCAAGTCA+AGG + chr8.2:52036261-52036280 None:intergenic 50.0%
TATGGACCAAGTCAAGGCCT+TGG + chr8.2:52036255-52036274 None:intergenic 50.0%
! AAGGTTTTGCCCGGCAAGAA+TGG - chr8.2:52035865-52035884 MS.gene035421:CDS 50.0%
! CCTGTTATCCTGAGCTTTGG+AGG + chr8.2:52036697-52036716 None:intergenic 50.0%
!! TGACTTGGTCCATACGCTAC+TGG - chr8.2:52036261-52036280 MS.gene035421:CDS 50.0%
GATGGCTTCTGCTTGGCAAG+TGG - chr8.2:52037368-52037387 MS.gene035421:CDS 55.0%
GCCAGTCTCGGTATGAACCA+AGG - chr8.2:52036235-52036254 MS.gene035421:CDS 55.0%
GCCTTGGTTCATACCGAGAC+TGG + chr8.2:52036239-52036258 None:intergenic 55.0%
!! CCGAGACTGGCTTGTGATTG+TGG + chr8.2:52036226-52036245 None:intergenic 55.0%
AACCTCGCTTCGAGGCCTGA+GGG - chr8.2:52036670-52036689 MS.gene035421:CDS 60.0%
CGGCACAAGCCAGTAGCGTA+TGG + chr8.2:52036273-52036292 None:intergenic 60.0%
GTCTCTGCAACCAACGGCCT+CGG + chr8.2:52036293-52036312 None:intergenic 60.0%
!! TACGCTACTGGCTTGTGCCG+AGG - chr8.2:52036273-52036292 MS.gene035421:CDS 60.0%
ACCTCGCTTCGAGGCCTGAG+GGG - chr8.2:52036671-52036690 MS.gene035421:CDS 65.0%
CTGAGGGGCCTCCAAAGCTC+AGG - chr8.2:52036686-52036705 MS.gene035421:CDS 65.0%
GAACCTCGCTTCGAGGCCTG+AGG - chr8.2:52036669-52036688 MS.gene035421:CDS 65.0%
!! CTGAGCTTTGGAGGCCCCTC+AGG + chr8.2:52036688-52036707 None:intergenic 65.0%
! CTGGCTTGTGCCGAGGCCGT+TGG - chr8.2:52036280-52036299 MS.gene035421:CDS 70.0%
GCCCCTCAGGCCTCGAAGCG+AGG + chr8.2:52036675-52036694 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 52035797 52037422 52035797 ID=MS.gene035421
chr8.2 mRNA 52035797 52037422 52035797 ID=MS.gene035421.t1;Parent=MS.gene035421
chr8.2 exon 52035797 52037422 52035797 ID=MS.gene035421.t1.exon1;Parent=MS.gene035421.t1
chr8.2 CDS 52035797 52037422 52035797 ID=cds.MS.gene035421.t1;Parent=MS.gene035421.t1
Gene Sequence

>MS.gene035421

ATGGAAGACTTAAACATGTGGCTTTCCTTCTATATGAATGAAAGTGTCAACCTTGATTTCCCTATTAGAAGGTTTTGCCCGGCAAGAATGGAACAAGATCAAGAAGGAGATTGCCATGACCAAGAGACAGTTCTTGATGATGTTGAATTGTGTACTTTTGATACTACCACCACTCCTTGTTTATTACCTTCTTCAGAAGTTGATGACTTTGTTGATAGCTTCATCAACATGGATCAATATGAAGATCATGAGTCTCAAGAGAAGAATCATCAAAGTCAAAGTTTTGATCATCACTTTTTGGTGAATGATGATGTCTTTGGATATGATCCAACCACAATGGAGGATGAAGAATTTGAGATCTGCGAAAATACAACAATCACAAATGAAGACGAAGAAGTTGAAATGAACGGCAATTTTGGTGCTATTCCACAATCACAAGCCAGTCTCGGTATGAACCAAGGCCTTGACTTGGTCCATACGCTACTGGCTTGTGCCGAGGCCGTTGGTTGCAGAGACAACAATCAGGCGAATTTGTTACTTGGTAAAATTTTGGCTTTGTCAAGTCCTTCAGGAGATTCGTTACAAAGAGTCTCCTACTGCTTTGCCACAGCATTGAAGTGTAGGTTATCACTTTATCCACATAATGTATTTTCAAATAGTACACTCACAACAAGTACTTCTAATGATGTTTCTTTGATTACTAGGGAGAATAAGTTGGAAGCTTTTCAACTTCTTTACCAAACAACACCTTATATTACTTTTGGTTTCATGGCTGCAAATGAAGCTATATGCCAAGGATCAAAAGGAAAATGCTCAATACACATAATTGATTTGGGAATGGAGCATGCTCTTCAATGGCCTTCGCTAATAAGGAACCTCGCTTCGAGGCCTGAGGGGCCTCCAAAGCTCAGGATAACAGGATTCAGTACGAATGAAGAAAACAACACAAAACTTCGCGCTAGCATGAATTTGCTTGTAGAGGAAGCACTCTCATTGGGAATAGTCCTTGAATTTCGCATAATATCAGAGCCGACAACTCCTTCTCTTCTTACAATCGACAACCTCAACTTGAGAGAAGGTGAATCTTTATTTGTCAATAGCATCTTGAAATTGCACAAATATGTGAAAGAAAGTAGAGGTTATCTAAAATCAATTCTTCAGTCAATCAAGAAGCTAAGTCCAATTGCACTCACAGTTGTCGAGCAAGACACGAACCACAATGGACCTTTCTTTCTTGGTAGGTTTCTAGAATCACTTCACTATTACTCAGCAATATTTGATTCTCTTGAGGCAAGTATGCCAAGAAATAGTCCAATCAGGATGAAAATAGAGAGAGTACATTTTGCAGAAGAAATTTGCAACATTGTAGCTTGTGAGGGTCCTGATAGAATGGAGAGACATGAAAGAGTAGATCAATGGAGAAGACAATTAGGAAGAGCAGGATTTCAAGTAATGCCACTCAAGTGTACAAGTCAAGCTAGAATGATGCTTTCTGTTTATGATTGTGATGGATATACTTTGTCATGTGAAAAGGGTTGCCTTTTACTTGGTTGGAAAGGAAGGTCTATTATGATGGCTTCTGCTTGGCAAGTGGCAAATTTGTCATATACTGATAGAGCCTCTTAA
Protein sequence

>MS.gene035421.t1

MEDLNMWLSFYMNESVNLDFPIRRFCPARMEQDQEGDCHDQETVLDDVELCTFDTTTTPCLLPSSEVDDFVDSFINMDQYEDHESQEKNHQSQSFDHHFLVNDDVFGYDPTTMEDEEFEICENTTITNEDEEVEMNGNFGAIPQSQASLGMNQGLDLVHTLLACAEAVGCRDNNQANLLLGKILALSSPSGDSLQRVSYCFATALKCRLSLYPHNVFSNSTLTTSTSNDVSLITRENKLEAFQLLYQTTPYITFGFMAANEAICQGSKGKCSIHIIDLGMEHALQWPSLIRNLASRPEGPPKLRITGFSTNEENNTKLRASMNLLVEEALSLGIVLEFRIISEPTTPSLLTIDNLNLREGESLFVNSILKLHKYVKESRGYLKSILQSIKKLSPIALTVVEQDTNHNGPFFLGRFLESLHYYSAIFDSLEASMPRNSPIRMKIERVHFAEEICNIVACEGPDRMERHERVDQWRRQLGRAGFQVMPLKCTSQARMMLSVYDCDGYTLSCEKGCLLLGWKGRSIMMASAWQVANLSYTDRAS