Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035428.t1 | XP_013465623.1 | 80.6 | 139 | 15 | 2 | 1 | 127 | 1 | 139 | 1.70E-52 | 215.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035428.t1 | Q9FKK2 | 35.8 | 137 | 69 | 4 | 8 | 125 | 3 | 139 | 2.7e-11 | 69.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035428.t1 | A0A072VC91 | 80.6 | 139 | 15 | 2 | 1 | 127 | 1 | 139 | 1.2e-52 | 215.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene035428.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035428.t1 | MTR_2g105290 | 80.576 | 139 | 15 | 2 | 1 | 127 | 1 | 139 | 1.56e-75 | 222 |
| MS.gene035428.t1 | MTR_4g127140 | 71.429 | 140 | 27 | 3 | 1 | 127 | 1 | 140 | 2.48e-54 | 168 |
| MS.gene035428.t1 | MTR_8g086290 | 56.115 | 139 | 49 | 2 | 1 | 127 | 1 | 139 | 4.48e-47 | 150 |
| MS.gene035428.t1 | MTR_7g028448 | 53.957 | 139 | 44 | 4 | 1 | 127 | 1 | 131 | 3.34e-36 | 122 |
| MS.gene035428.t1 | MTR_4g036915 | 53.957 | 139 | 44 | 4 | 1 | 127 | 1 | 131 | 3.34e-36 | 122 |
| MS.gene035428.t1 | MTR_3g052920 | 36.691 | 139 | 66 | 4 | 4 | 123 | 1 | 136 | 1.01e-22 | 89.4 |
| MS.gene035428.t1 | MTR_1g105910 | 37.410 | 139 | 65 | 4 | 4 | 123 | 1 | 136 | 2.09e-22 | 88.6 |
| MS.gene035428.t1 | MTR_1g012570 | 36.691 | 139 | 66 | 4 | 4 | 123 | 1 | 136 | 5.14e-22 | 87.4 |
| MS.gene035428.t1 | MTR_1g106070 | 35.000 | 140 | 69 | 4 | 4 | 124 | 1 | 137 | 1.00e-21 | 85.5 |
| MS.gene035428.t1 | MTR_1g105920 | 35.971 | 139 | 67 | 4 | 4 | 123 | 1 | 136 | 1.61e-21 | 86.3 |
| MS.gene035428.t1 | MTR_8g043650 | 37.778 | 135 | 62 | 5 | 8 | 123 | 5 | 136 | 2.63e-20 | 83.2 |
| MS.gene035428.t1 | MTR_2g030740 | 35.971 | 139 | 67 | 5 | 4 | 123 | 1 | 136 | 3.31e-20 | 82.8 |
| MS.gene035428.t1 | MTR_3g052870 | 36.434 | 129 | 60 | 4 | 4 | 113 | 1 | 126 | 5.61e-20 | 81.6 |
| MS.gene035428.t1 | MTR_1g108510 | 35.252 | 139 | 68 | 4 | 4 | 123 | 1 | 136 | 3.59e-18 | 77.4 |
| MS.gene035428.t1 | MTR_3g030770 | 34.532 | 139 | 69 | 4 | 4 | 123 | 1 | 136 | 5.18e-18 | 77.0 |
| MS.gene035428.t1 | MTR_2g093190 | 34.532 | 139 | 69 | 4 | 4 | 123 | 1 | 136 | 1.10e-17 | 75.9 |
| MS.gene035428.t1 | MTR_1g075600 | 34.286 | 140 | 68 | 4 | 4 | 123 | 1 | 136 | 6.50e-17 | 73.9 |
| MS.gene035428.t1 | MTR_1g108580 | 34.074 | 135 | 67 | 4 | 8 | 123 | 5 | 136 | 9.59e-17 | 73.6 |
| MS.gene035428.t1 | MTR_0121s0100 | 35.115 | 131 | 63 | 4 | 4 | 115 | 1 | 128 | 9.80e-17 | 71.2 |
| MS.gene035428.t1 | MTR_1g077360 | 34.532 | 139 | 69 | 4 | 4 | 123 | 1 | 136 | 1.02e-16 | 73.6 |
| MS.gene035428.t1 | MTR_5g045560 | 48.052 | 77 | 28 | 2 | 4 | 68 | 1 | 77 | 4.76e-16 | 71.6 |
| MS.gene035428.t1 | MTR_0121s0080 | 34.559 | 136 | 67 | 5 | 7 | 123 | 4 | 136 | 9.84e-16 | 70.9 |
| MS.gene035428.t1 | MTR_3g080940 | 33.333 | 132 | 72 | 3 | 8 | 123 | 5 | 136 | 3.10e-15 | 69.3 |
| MS.gene035428.t1 | MTR_8g046350 | 32.576 | 132 | 74 | 4 | 11 | 128 | 1 | 131 | 6.32e-15 | 67.4 |
| MS.gene035428.t1 | MTR_8g079502 | 35.200 | 125 | 64 | 4 | 11 | 121 | 1 | 122 | 4.33e-14 | 65.5 |
| MS.gene035428.t1 | MTR_5g053390 | 45.455 | 77 | 30 | 2 | 4 | 68 | 1 | 77 | 7.72e-14 | 65.9 |
| MS.gene035428.t1 | MTR_1g105905 | 32.787 | 122 | 60 | 4 | 21 | 123 | 1 | 119 | 1.15e-13 | 65.1 |
| MS.gene035428.t1 | MTR_2g085280 | 34.307 | 137 | 65 | 6 | 7 | 123 | 4 | 135 | 1.87e-13 | 64.7 |
| MS.gene035428.t1 | MTR_2g085250 | 34.307 | 137 | 65 | 6 | 7 | 123 | 4 | 135 | 1.89e-13 | 64.7 |
| MS.gene035428.t1 | MTR_1g047550 | 38.384 | 99 | 47 | 3 | 11 | 95 | 1 | 99 | 4.52e-13 | 62.8 |
| MS.gene035428.t1 | MTR_4g131030 | 39.000 | 100 | 47 | 3 | 11 | 96 | 1 | 100 | 5.48e-13 | 63.2 |
| MS.gene035428.t1 | MTR_5g055100 | 45.455 | 77 | 30 | 2 | 4 | 68 | 1 | 77 | 5.86e-13 | 63.5 |
| MS.gene035428.t1 | MTR_3g030780 | 34.815 | 135 | 64 | 6 | 4 | 118 | 1 | 131 | 6.36e-13 | 61.6 |
| MS.gene035428.t1 | MTR_4g094632 | 33.594 | 128 | 62 | 4 | 11 | 116 | 1 | 127 | 5.82e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035428.t1 | AT5G60440 | 35.252 | 139 | 71 | 4 | 8 | 127 | 3 | 141 | 7.58e-16 | 72.0 |
| MS.gene035428.t1 | AT1G65360 | 34.848 | 132 | 71 | 4 | 8 | 124 | 3 | 134 | 9.73e-15 | 68.2 |
| MS.gene035428.t1 | AT1G01530 | 34.783 | 138 | 63 | 5 | 7 | 123 | 2 | 133 | 1.86e-13 | 65.1 |
| MS.gene035428.t1 | AT2G24840 | 33.333 | 132 | 71 | 5 | 9 | 123 | 60 | 191 | 4.84e-13 | 64.3 |
| MS.gene035428.t1 | AT1G29962 | 34.043 | 141 | 64 | 6 | 8 | 123 | 4 | 140 | 2.04e-12 | 61.2 |
| MS.gene035428.t1 | AT5G10140 | 38.144 | 97 | 45 | 3 | 11 | 94 | 1 | 95 | 4.82e-12 | 60.1 |
| MS.gene035428.t1 | AT5G10140 | 38.144 | 97 | 45 | 3 | 11 | 94 | 1 | 95 | 8.42e-12 | 59.7 |
| MS.gene035428.t1 | AT5G10140 | 38.144 | 97 | 45 | 3 | 11 | 94 | 1 | 95 | 9.40e-12 | 59.7 |
| MS.gene035428.t1 | AT3G66656 | 35.433 | 127 | 50 | 5 | 11 | 111 | 1 | 121 | 9.97e-12 | 59.3 |
| MS.gene035428.t1 | AT5G10140 | 38.144 | 97 | 45 | 3 | 11 | 94 | 1 | 95 | 1.42e-11 | 59.3 |
| MS.gene035428.t1 | AT1G28460 | 32.609 | 138 | 67 | 5 | 8 | 123 | 4 | 137 | 1.50e-11 | 58.9 |
| MS.gene035428.t1 | AT4G36590 | 52.459 | 61 | 17 | 2 | 12 | 60 | 7 | 67 | 5.06e-11 | 58.5 |
Find 24 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAGAAGATGTTCAAAATA+TGG | 0.310043 | 8.2:-51956979 | MS.gene035428:CDS |
| TTTCACCATCACCATGGATA+TGG | 0.310081 | 8.2:-51957346 | None:intergenic |
| CCTTCTTGATCTCAATCTTC+TGG | 0.316747 | 8.2:+51957295 | None:intergenic |
| GCCCCATGTTCTTCTTTCCT+TGG | 0.338604 | 8.2:+51957319 | None:intergenic |
| TGAGAAAGTGACAAGTTTGT+TGG | 0.353316 | 8.2:+51957260 | None:intergenic |
| GGTGACAAACTCTACTGTTT+TGG | 0.359262 | 8.2:-51957152 | MS.gene035428:CDS |
| CAAAGGAACCACTGAGTTTG+AGG | 0.435112 | 8.2:-51957093 | MS.gene035428:CDS |
| CCACTGAGTTTGAGGATTCA+AGG | 0.438826 | 8.2:-51957085 | MS.gene035428:CDS |
| TTCCTTTGATGTAGCAATCA+AGG | 0.470950 | 8.2:+51957108 | None:intergenic |
| AAATTACAATAGACAATATG+AGG | 0.503552 | 8.2:-51957036 | MS.gene035428:CDS |
| TCCTTGGTTAACCATATCCA+TGG | 0.512893 | 8.2:+51957335 | None:intergenic |
| GTCCTTGATTGCTACATCAA+AGG | 0.537958 | 8.2:-51957110 | MS.gene035428:CDS |
| ATCAAGGACAACGTTAGTGT+CGG | 0.552254 | 8.2:+51957124 | None:intergenic |
| TCAAGGACAACGTTAGTGTC+GGG | 0.553835 | 8.2:+51957125 | None:intergenic |
| AACCAAGGAAAGAAGAACAT+GGG | 0.621148 | 8.2:-51957321 | MS.gene035428:CDS |
| GGCACAAAAGACGTTGGCGA+CGG | 0.625112 | 8.2:-51957012 | MS.gene035428:CDS |
| TAACCAAGGAAAGAAGAACA+TGG | 0.625296 | 8.2:-51957322 | MS.gene035428:CDS |
| CCAGAAGATTGAGATCAAGA+AGG | 0.650458 | 8.2:-51957295 | MS.gene035428:CDS |
| CCTTGAATCCTCAAACTCAG+TGG | 0.659592 | 8.2:+51957085 | None:intergenic |
| ACCATGGATATGGTTAACCA+AGG | 0.659881 | 8.2:-51957336 | MS.gene035428:CDS |
| ACAGTAGAGTTTGTCACCAG+CGG | 0.663634 | 8.2:+51957157 | None:intergenic |
| TGAGGAGGCACAAAAGACGT+TGG | 0.669475 | 8.2:-51957018 | MS.gene035428:CDS |
| ACCAAGGAAAGAAGAACATG+GGG | 0.676392 | 8.2:-51957320 | MS.gene035428:CDS |
| TTACAATAGACAATATGAGG+AGG | 0.685706 | 8.2:-51957033 | MS.gene035428:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTACAATAGACAATATG+AGG | - | chr8.2:51957226-51957245 | MS.gene035428:intron | 20.0% |
| ! | ACAAGAAGATGTTCAAAATA+TGG | - | chr8.2:51957283-51957302 | MS.gene035428:CDS | 25.0% |
| !! | CTATTGTAATTTTTGCAAGT+CGG | + | chr8.2:51957218-51957237 | None:intergenic | 25.0% |
| ACTTTCTCAAAAAGAAGACA+AGG | - | chr8.2:51957016-51957035 | MS.gene035428:CDS | 30.0% | |
| TTACAATAGACAATATGAGG+AGG | - | chr8.2:51957229-51957248 | MS.gene035428:intron | 30.0% | |
| ! | ATGGCTAAGATTTTCAACAA+GGG | - | chr8.2:51957302-51957321 | MS.gene035428:CDS | 30.0% |
| ! | TATGGCTAAGATTTTCAACA+AGG | - | chr8.2:51957301-51957320 | MS.gene035428:CDS | 30.0% |
| AACCAAGGAAAGAAGAACAT+GGG | - | chr8.2:51956941-51956960 | MS.gene035428:CDS | 35.0% | |
| TAACCAAGGAAAGAAGAACA+TGG | - | chr8.2:51956940-51956959 | MS.gene035428:CDS | 35.0% | |
| TGAGAAAGTGACAAGTTTGT+TGG | + | chr8.2:51957005-51957024 | None:intergenic | 35.0% | |
| TTCCTTTGATGTAGCAATCA+AGG | + | chr8.2:51957157-51957176 | None:intergenic | 35.0% | |
| AAAACACAATGATGGCTGCA+TGG | + | chr8.2:51957084-51957103 | None:intergenic | 40.0% | |
| ACCAAGGAAAGAAGAACATG+GGG | - | chr8.2:51956942-51956961 | MS.gene035428:CDS | 40.0% | |
| ATCAAGGACAACGTTAGTGT+CGG | + | chr8.2:51957141-51957160 | None:intergenic | 40.0% | |
| CCAGAAGATTGAGATCAAGA+AGG | - | chr8.2:51956967-51956986 | MS.gene035428:CDS | 40.0% | |
| CCTTCTTGATCTCAATCTTC+TGG | + | chr8.2:51956970-51956989 | None:intergenic | 40.0% | |
| GTCCTTGATTGCTACATCAA+AGG | - | chr8.2:51957152-51957171 | MS.gene035428:CDS | 40.0% | |
| ! | AGATTTTCAACAAGGGTGAC+TGG | - | chr8.2:51957309-51957328 | MS.gene035428:CDS | 40.0% |
| ! | GGTGACAAACTCTACTGTTT+TGG | - | chr8.2:51957110-51957129 | MS.gene035428:CDS | 40.0% |
| ACAGTAGAGTTTGTCACCAG+CGG | + | chr8.2:51957108-51957127 | None:intergenic | 45.0% | |
| CAAAGGAACCACTGAGTTTG+AGG | - | chr8.2:51957169-51957188 | MS.gene035428:CDS | 45.0% | |
| CCACTGAGTTTGAGGATTCA+AGG | - | chr8.2:51957177-51957196 | MS.gene035428:intron | 45.0% | |
| CCAGCGGAAAAACACAATGA+TGG | + | chr8.2:51957092-51957111 | None:intergenic | 45.0% | |
| CCTTGAATCCTCAAACTCAG+TGG | + | chr8.2:51957180-51957199 | None:intergenic | 45.0% | |
| TCAAGGACAACGTTAGTGTC+GGG | + | chr8.2:51957140-51957159 | None:intergenic | 45.0% | |
| ! | CCATCATTGTGTTTTTCCGC+TGG | - | chr8.2:51957089-51957108 | MS.gene035428:CDS | 45.0% |
| ! | TTTTCAACAAGGGTGACTGG+TGG | - | chr8.2:51957312-51957331 | MS.gene035428:CDS | 45.0% |
| GCCCCATGTTCTTCTTTCCT+TGG | + | chr8.2:51956946-51956965 | None:intergenic | 50.0% | |
| ! | TGAGGAGGCACAAAAGACGT+TGG | - | chr8.2:51957244-51957263 | MS.gene035428:CDS | 50.0% |
| GGCACAAAAGACGTTGGCGA+CGG | - | chr8.2:51957250-51957269 | MS.gene035428:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 51956929 | 51957355 | 51956929 | ID=MS.gene035428 |
| chr8.2 | mRNA | 51956929 | 51957355 | 51956929 | ID=MS.gene035428.t1;Parent=MS.gene035428 |
| chr8.2 | exon | 51957233 | 51957355 | 51957233 | ID=MS.gene035428.t1.exon1;Parent=MS.gene035428.t1 |
| chr8.2 | CDS | 51957233 | 51957355 | 51957233 | ID=cds.MS.gene035428.t1;Parent=MS.gene035428.t1 |
| chr8.2 | exon | 51956929 | 51957195 | 51956929 | ID=MS.gene035428.t1.exon2;Parent=MS.gene035428.t1 |
| chr8.2 | CDS | 51956929 | 51957195 | 51956929 | ID=cds.MS.gene035428.t1;Parent=MS.gene035428.t1 |
| Gene Sequence |
| Protein sequence |
