AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035466

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035466.t1 MTR_1g047060 54.000 100 37 3 110 207 1 93 2.76e-27 101
MS.gene035466.t1 MTR_4g101680 33.758 157 81 6 4 146 7 154 2.54e-17 80.5
MS.gene035466.t1 MTR_4g094302 29.508 183 91 7 4 172 11 169 3.68e-17 77.4
MS.gene035466.t1 MTR_5g021710 33.117 154 86 5 4 146 8 155 8.72e-17 79.0
MS.gene035466.t1 MTR_1g069805 34.641 153 82 7 4 146 10 154 3.13e-16 76.6
MS.gene035466.t1 MTR_7g100990 33.750 160 80 7 4 145 11 162 5.90e-16 76.3
MS.gene035466.t1 MTR_1g008740 33.758 157 86 6 2 146 25 175 9.48e-16 75.9
MS.gene035466.t1 MTR_2g080010 31.737 167 83 7 4 146 15 174 1.22e-15 75.5
MS.gene035466.t1 MTR_1g008740 33.758 157 86 6 2 146 11 161 1.23e-15 75.5
MS.gene035466.t1 MTR_8g076110 34.194 155 83 6 4 146 6 153 1.46e-15 75.1
MS.gene035466.t1 MTR_4g094302 33.333 129 77 4 4 132 11 130 1.91e-15 72.0
MS.gene035466.t1 MTR_5g041940 30.625 160 85 6 4 146 3 153 2.50e-15 73.6
MS.gene035466.t1 MTR_8g023840 34.416 154 80 8 4 145 11 155 3.06e-15 74.7
MS.gene035466.t1 MTR_4g089135 35.556 135 74 5 4 132 15 142 4.16e-15 73.9
MS.gene035466.t1 MTR_2g093810 31.875 160 83 6 4 145 14 165 4.91e-15 73.6
MS.gene035466.t1 MTR_4g035590 29.798 198 112 10 4 188 8 191 5.52e-15 73.9
MS.gene035466.t1 MTR_3g109340 33.121 157 86 6 2 146 20 169 6.71e-15 73.6
MS.gene035466.t1 MTR_3g093040 34.161 161 76 10 4 146 108 256 7.06e-15 73.2
MS.gene035466.t1 MTR_3g093050 33.742 163 80 10 1 146 19 170 1.24e-14 73.2
MS.gene035466.t1 MTR_8g023900 33.544 158 82 8 2 145 10 158 1.70e-14 72.8
MS.gene035466.t1 MTR_8g023900 29.255 188 108 8 7 180 196 372 1.34e-11 64.3
MS.gene035466.t1 MTR_3g093050 34.375 160 77 10 4 146 22 170 1.91e-14 72.8
MS.gene035466.t1 MTR_8g467490 25.630 238 132 9 2 203 12 240 2.54e-14 72.0
MS.gene035466.t1 MTR_8g023860 31.410 156 88 6 1 145 9 156 4.84e-14 71.2
MS.gene035466.t1 MTR_3g064580 33.962 159 80 9 4 146 6 155 4.87e-14 71.6
MS.gene035466.t1 MTR_1g090720 34.356 163 79 7 4 146 42 196 1.31e-13 70.1
MS.gene035466.t1 MTR_5g014300 30.769 195 109 10 4 180 6 192 1.47e-13 69.7
MS.gene035466.t1 MTR_6g011860 31.515 165 83 9 4 146 15 171 3.18e-13 68.9
MS.gene035466.t1 MTR_5g069030 34.161 161 76 10 4 146 25 173 3.70e-13 68.9
MS.gene035466.t1 MTR_3g116070 31.293 147 89 5 4 144 23 163 4.39e-13 68.2
MS.gene035466.t1 MTR_7g005280 31.613 155 83 7 4 146 15 158 8.07e-13 66.6
MS.gene035466.t1 MTR_5g076850 32.704 159 81 9 4 146 6 154 8.34e-13 68.2
MS.gene035466.t1 MTR_8g024480 27.381 168 88 7 4 146 15 173 1.85e-12 66.6
MS.gene035466.t1 MTR_6g084430 32.051 156 86 7 2 146 3 149 2.71e-12 65.9
MS.gene035466.t1 MTR_2g079990 35.294 136 71 7 4 132 16 141 2.85e-12 65.9
MS.gene035466.t1 MTR_7g085220 28.571 168 87 8 4 146 8 167 7.60e-12 64.7
MS.gene035466.t1 MTR_2g078700 30.818 159 86 6 4 146 23 173 1.50e-11 63.9
MS.gene035466.t1 MTR_3g096400 29.518 166 93 5 4 152 7 165 3.04e-11 61.6
MS.gene035466.t1 MTR_3g070030 28.824 170 93 7 4 154 19 179 9.29e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035466.t1 AT4G36160 33.544 158 88 5 4 150 10 161 1.03e-18 84.7
MS.gene035466.t1 AT4G36160 33.544 158 88 5 4 150 10 161 1.03e-18 84.7
MS.gene035466.t1 AT4G36160 33.544 158 88 5 4 150 14 165 1.17e-18 84.3
MS.gene035466.t1 AT1G71930 33.121 157 88 5 4 149 9 159 6.78e-18 81.6
MS.gene035466.t1 AT1G71930 33.121 157 88 5 4 149 9 159 6.78e-18 81.6
MS.gene035466.t1 AT2G24430 35.669 157 82 6 2 146 14 163 1.54e-17 80.9
MS.gene035466.t1 AT2G24430 35.669 157 82 6 2 146 14 163 1.54e-17 80.9
MS.gene035466.t1 AT2G18060 33.117 154 86 5 4 146 9 156 2.98e-17 80.5
MS.gene035466.t1 AT2G18060 33.117 154 86 5 4 146 9 156 2.98e-17 80.5
MS.gene035466.t1 AT4G28530 33.133 166 85 5 4 147 10 171 3.34e-16 77.4
MS.gene035466.t1 AT1G69490 32.500 160 82 7 4 146 9 159 6.17e-16 75.5
MS.gene035466.t1 AT1G26870 33.974 156 86 6 2 149 14 160 7.19e-16 76.6
MS.gene035466.t1 AT3G10490 33.544 158 82 7 4 146 27 176 1.86e-15 73.9
MS.gene035466.t1 AT4G10350 33.962 159 80 9 4 146 9 158 3.96e-15 74.3
MS.gene035466.t1 AT2G27300 29.452 146 93 4 4 145 14 153 4.11e-15 73.9
MS.gene035466.t1 AT4G17980 33.333 159 82 7 4 145 6 157 4.65e-15 73.2
MS.gene035466.t1 AT4G17980 33.125 160 83 7 3 145 5 157 5.82e-15 73.2
MS.gene035466.t1 AT5G63790 33.333 162 80 7 4 147 50 201 1.15e-14 72.4
MS.gene035466.t1 AT5G63790 33.333 162 80 7 4 147 60 211 1.35e-14 72.4
MS.gene035466.t1 AT3G03200 34.375 160 78 9 4 146 6 155 1.51e-14 73.2
MS.gene035466.t1 AT1G54330 32.026 153 87 6 4 146 6 151 1.86e-14 72.0
MS.gene035466.t1 AT5G64530 33.588 131 74 4 4 134 3 120 2.61e-14 69.7
MS.gene035466.t1 AT5G64530 33.588 131 74 4 4 134 3 120 2.94e-14 68.9
MS.gene035466.t1 AT3G10480 33.121 157 83 7 4 146 27 175 5.06e-14 71.2
MS.gene035466.t1 AT4G35580 31.902 163 89 8 1 149 6 160 6.73e-14 71.2
MS.gene035466.t1 AT4G35580 31.902 163 89 8 1 149 6 160 6.79e-14 71.2
MS.gene035466.t1 AT4G35580 31.902 163 89 8 1 149 6 160 8.03e-14 70.9
MS.gene035466.t1 AT5G39610 32.278 158 83 7 4 146 20 168 1.16e-13 69.3
MS.gene035466.t1 AT1G61110 34.058 138 75 6 4 134 16 144 1.80e-13 69.3
MS.gene035466.t1 AT5G04410 31.646 158 84 6 4 146 9 157 2.25e-13 69.7
MS.gene035466.t1 AT5G39820 33.835 133 75 5 6 132 22 147 3.29e-13 68.6
MS.gene035466.t1 AT1G32770 28.729 181 85 8 4 149 16 187 4.83e-13 68.2
MS.gene035466.t1 AT3G10500 32.278 158 83 7 4 146 9 157 4.87e-13 68.6
MS.gene035466.t1 AT2G46770 26.368 201 107 8 4 175 16 204 7.18e-13 67.8
MS.gene035466.t1 AT3G15170 31.447 159 85 7 3 145 19 169 7.53e-13 67.4
MS.gene035466.t1 AT3G18400 31.646 158 87 8 2 147 3 151 1.04e-12 67.0
MS.gene035466.t1 AT3G29035 31.447 159 83 7 4 146 24 172 1.34e-12 66.6
MS.gene035466.t1 AT4G27410 29.240 171 86 7 4 146 14 177 1.77e-12 66.2
MS.gene035466.t1 AT5G61430 32.278 158 83 7 4 146 16 164 2.21e-12 66.2
MS.gene035466.t1 AT3G15510 29.814 161 82 7 10 146 23 176 2.36e-12 66.2
MS.gene035466.t1 AT5G22290 28.571 147 90 6 6 145 23 161 2.62e-12 65.9
MS.gene035466.t1 AT5G24590 30.380 158 87 7 4 146 13 162 4.15e-12 65.9
MS.gene035466.t1 AT2G17040 30.323 155 82 7 6 146 8 150 4.22e-12 65.1
MS.gene035466.t1 AT1G32870 32.331 133 79 5 4 132 10 135 8.11e-12 65.1
MS.gene035466.t1 AT3G04060 33.333 159 81 9 4 146 20 169 8.59e-12 64.3
MS.gene035466.t1 AT1G32870 32.331 133 79 5 4 132 10 135 9.02e-12 64.7
MS.gene035466.t1 AT1G32870 32.331 133 79 5 4 132 46 171 1.30e-11 64.3
MS.gene035466.t1 AT5G53950 29.814 161 89 6 2 146 15 167 2.13e-11 63.5
MS.gene035466.t1 AT5G46590 32.374 139 80 6 4 135 6 137 2.33e-11 62.8
MS.gene035466.t1 AT5G04395 31.333 150 80 6 4 135 28 172 4.14e-11 60.8

Find 49 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACTAATGCTTTCAATTA+TGG 0.184656 6.1:+33936408 MS.gene035466:CDS
CAATGGTTCTGACTTGATTA+AGG 0.258416 6.1:+33934179 MS.gene035466:CDS
GGCAATGTAAGATTGGATTT+TGG 0.279853 6.1:+33934313 MS.gene035466:CDS
CCTTGGGTGACGGAAGAAAA+AGG 0.350861 6.1:-33934250 None:intergenic
ATTGAAAGCATTAGTTGCTC+TGG 0.380713 6.1:-33936403 None:intergenic
ATGGATCTGGATGTTGAGTT+TGG 0.395501 6.1:+33934639 MS.gene035466:CDS
CCTCATCTGCTCATCAACTT+TGG 0.414860 6.1:-33934292 None:intergenic
GAAGAGGTTCAAAATTTCAA+TGG 0.434760 6.1:+33934162 MS.gene035466:CDS
GGAGTAAGACGCAATTCAAA+TGG 0.441267 6.1:-33934225 None:intergenic
GCTGTTCGAGACATTATTGT+TGG 0.457958 6.1:+33934348 MS.gene035466:CDS
GCAATTCAAATGGGTCATAC+TGG 0.459319 6.1:-33934215 None:intergenic
CAAATGGGTCATACTGGTAC+AGG 0.462094 6.1:-33934209 None:intergenic
CAGATGAGGCAATGTAAGAT+TGG 0.462721 6.1:+33934306 MS.gene035466:CDS
TTGAAAGCATTAGTTGCTCT+GGG 0.473709 6.1:-33936402 None:intergenic
CGAAGTTACTCAGAAACCTA+TGG 0.482750 6.1:+33934716 MS.gene035466:CDS
GAATTGCTTCCACCTGGTGT+TGG 0.483908 6.1:+33934084 MS.gene035466:CDS
AACACACGACAAAGAACAAA+AGG 0.484780 6.1:-33934600 None:intergenic
TCAATTAATTTACACAAGAT+CGG 0.501330 6.1:-33936452 None:intergenic
GTGAAGCAGTAAAATTCCCT+TGG 0.511128 6.1:-33934267 None:intergenic
AGAATGGCATCAAAACTAGT+TGG 0.512078 6.1:+33934433 MS.gene035466:CDS
CTGCCCACCAGAGATGGATC+TGG 0.513629 6.1:+33934626 MS.gene035466:CDS
TCAACATCCAGATCCATCTC+TGG 0.528153 6.1:-33934633 None:intergenic
CACTCTTGCTAACAATCCTC+AGG 0.528444 6.1:+33934470 MS.gene035466:CDS
GTTGATGAAGGTGATGCTAC+TGG 0.539280 6.1:+33934669 MS.gene035466:CDS
AGTAAAATTCCCTTGGGTGA+CGG 0.539767 6.1:-33934260 None:intergenic
GAAGGTGATGCTACTGGAGA+TGG 0.546862 6.1:+33934675 MS.gene035466:CDS
GAGTAAGACGCAATTCAAAT+GGG 0.549774 6.1:-33934224 None:intergenic
TGAAGCAGTAAAATTCCCTT+GGG 0.559532 6.1:-33934266 None:intergenic
CCAAAGTTGATGAGCAGATG+AGG 0.560720 6.1:+33934292 MS.gene035466:CDS
ACATCCAGATCCATCTCTGG+TGG 0.568257 6.1:-33934630 None:intergenic
GAAGTTACTCAGAAACCTAT+GGG 0.580237 6.1:+33934717 MS.gene035466:CDS
GAAGGGATGAAGCCAACACC+AGG 0.588550 6.1:-33934096 None:intergenic
TTTGGAGCAGATGTTGATGA+AGG 0.590115 6.1:+33934657 MS.gene035466:CDS
ATAGGAAATTCCGCTCAGAA+TGG 0.595921 6.1:+33934417 MS.gene035466:CDS
GTTTGACTGCCCACCAGAGA+TGG 0.602762 6.1:+33934620 MS.gene035466:CDS
TGTTGGAACTGATAACGTGT+TGG 0.614058 6.1:+33934365 MS.gene035466:CDS
TAACAAGGAGTTCATCAGAA+GGG 0.616296 6.1:-33934113 None:intergenic
GCAATGGAATTGCTTCCACC+TGG 0.624048 6.1:+33934078 None:intergenic
TGGAACTGATAACGTGTTGG+TGG 0.634013 6.1:+33934368 MS.gene035466:CDS
TGACGATGTTGTCTTGCACA+TGG 0.636821 6.1:+33936369 MS.gene035466:CDS
TGGCAAGGTAGTAGATAACA+AGG 0.641793 6.1:-33934128 None:intergenic
ATAACAAGGAGTTCATCAGA+AGG 0.650392 6.1:-33934114 None:intergenic
CATCCAGATCCATCTCTGGT+GGG 0.656418 6.1:-33934629 None:intergenic
GGGATGAAGCCAACACCAGG+TGG 0.665656 6.1:-33934093 None:intergenic
GGAACTGATAACGTGTTGGT+GGG 0.675682 6.1:+33934369 MS.gene035466:CDS
AAGTTACTCAGAAACCTATG+GGG 0.699471 6.1:+33934718 MS.gene035466:CDS
AGTGCTGCTGTTGAGCAACG+CGG 0.701822 6.1:+33934762 MS.gene035466:CDS
TGAAAGCATTAGTTGCTCTG+GGG 0.714933 6.1:-33936401 None:intergenic
AGTTACTCAGAAACCTATGG+GGG 0.738835 6.1:+33934719 MS.gene035466:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATTAAATTGTAATG+AGG - chr6.1:33935755-33935774 None:intergenic 10.0%
!! ATATGAATTAAAATAATCTT+AGG + chr6.1:33935664-33935683 MS.gene035466:intron 10.0%
!! TATTATTATTATTATGAGTT+AGG + chr6.1:33935249-33935268 MS.gene035466:intron 10.0%
!!! TTTTTTTTAAATTATTATGC+AGG + chr6.1:33934575-33934594 MS.gene035466:intron 10.0%
!! ATAATAATAATAATAGAGTC+AGG - chr6.1:33936134-33936153 None:intergenic 15.0%
!! CTTTGATTAAATAAAATCAA+CGG + chr6.1:33936061-33936080 MS.gene035466:intron 15.0%
!!! GTATTTTAATTATTCAGATA+TGG + chr6.1:33935891-33935910 MS.gene035466:intron 15.0%
!!! TTTTAATTCATATTCAATCA+TGG - chr6.1:33935657-33935676 None:intergenic 15.0%
!! AAAAAAATACATCACATGAA+TGG - chr6.1:33934928-33934947 None:intergenic 20.0%
!! AATACATTAAATAATCATCG+AGG - chr6.1:33934538-33934557 None:intergenic 20.0%
!! AATATAAAGAGAGATCAAAA+TGG - chr6.1:33934837-33934856 None:intergenic 20.0%
!! AGAAGAAGAATAATATTCAT+AGG - chr6.1:33935476-33935495 None:intergenic 20.0%
!! ATAAGATCAACAACATTAAT+TGG - chr6.1:33934862-33934881 None:intergenic 20.0%
!! CTTATGATAACAATTACAAT+TGG + chr6.1:33935565-33935584 MS.gene035466:intron 20.0%
!! TGATAATTCTCAAAACATAT+AGG + chr6.1:33935041-33935060 MS.gene035466:intron 20.0%
!!! AAAGAAACATGGATTAATTA+TGG - chr6.1:33935322-33935341 None:intergenic 20.0%
!!! AAGAAACATGGATTAATTAT+GGG - chr6.1:33935321-33935340 None:intergenic 20.0%
!!! CAACTTTTCATTTCATATTA+TGG + chr6.1:33936265-33936284 MS.gene035466:intron 20.0%
!!! CGTTGATTTTATTTAATCAA+AGG - chr6.1:33936063-33936082 None:intergenic 20.0%
!!! TCTCTTTTTATGTTCTATAT+AGG + chr6.1:33934399-33934418 MS.gene035466:CDS 20.0%
! AAAAACTCAAACTGAAAACA+TGG + chr6.1:33935395-33935414 MS.gene035466:intron 25.0%
! AAGAATAATATTCATAGGAG+TGG - chr6.1:33935471-33935490 None:intergenic 25.0%
! AATACTTCAACAATACATTC+TGG - chr6.1:33936297-33936316 None:intergenic 25.0%
! AGAACAAAGAGAATTATGAT+AGG + chr6.1:33935937-33935956 MS.gene035466:intron 25.0%
! AGAATAATATTCATAGGAGT+GGG - chr6.1:33935470-33935489 None:intergenic 25.0%
! ATACACAAGAAAAAGAAACA+TGG - chr6.1:33935333-33935352 None:intergenic 25.0%
! ATAGGATTATGTTTCCAATT+TGG + chr6.1:33935955-33935974 MS.gene035466:intron 25.0%
! ATAGTGTGTGATTGAAAAAA+AGG + chr6.1:33936002-33936021 MS.gene035466:intron 25.0%
! TAAAAGCAATAATTGTGTGT+GGG - chr6.1:33935367-33935386 None:intergenic 25.0%
! TAGGATTATGTTTCCAATTT+GGG + chr6.1:33935956-33935975 MS.gene035466:intron 25.0%
! TTACATTTAGTAGCAAAGAT+TGG - chr6.1:33934957-33934976 None:intergenic 25.0%
!! TCAAATTTCACAGATTTTGA+AGG + chr6.1:33934789-33934808 MS.gene035466:CDS 25.0%
!!! AACCTCTTCATTATTTTTGT+TGG - chr6.1:33934151-33934170 None:intergenic 25.0%
AATAATATTCATAGGAGTGG+GGG - chr6.1:33935468-33935487 None:intergenic 30.0%
AATGATTACCAAACCCAAAT+TGG - chr6.1:33935972-33935991 None:intergenic 30.0%
ATACGAGGAGTTAAATAAGA+GGG - chr6.1:33935512-33935531 None:intergenic 30.0%
ATTACAATTGGAGAGGAATA+TGG + chr6.1:33935577-33935596 MS.gene035466:intron 30.0%
CTAAAAGCAATAATTGTGTG+TGG - chr6.1:33935368-33935387 None:intergenic 30.0%
GAAGAGGTTCAAAATTTCAA+TGG + chr6.1:33934162-33934181 MS.gene035466:CDS 30.0%
GAATAATATTCATAGGAGTG+GGG - chr6.1:33935469-33935488 None:intergenic 30.0%
GATAACAATTACAATTGGAG+AGG + chr6.1:33935570-33935589 MS.gene035466:intron 30.0%
GCAACTAATGCTTTCAATTA+TGG + chr6.1:33936408-33936427 MS.gene035466:CDS 30.0%
TAAAATCAACGGTTGAGATT+TGG + chr6.1:33936072-33936091 MS.gene035466:intron 30.0%
TGCCAACAAAAATAATGAAG+AGG + chr6.1:33934146-33934165 MS.gene035466:CDS 30.0%
TGTCTACAAAATGAATCGTA+TGG + chr6.1:33935711-33935730 MS.gene035466:intron 30.0%
! AATTGGTTCAATTAGCATGT+AGG + chr6.1:33935834-33935853 MS.gene035466:intron 30.0%
! ATACTCAACGATTCTCTTTT+GGG + chr6.1:33935425-33935444 MS.gene035466:intron 30.0%
! ATGGAATATATTTTACCTGC+AGG - chr6.1:33934818-33934837 None:intergenic 30.0%
! CAAAATGAATCGTATGGTTT+TGG + chr6.1:33935717-33935736 MS.gene035466:intron 30.0%
! TGGAATATATTTTACCTGCA+GGG - chr6.1:33934817-33934836 None:intergenic 30.0%
! TTATGTTTCCAATTTGGGTT+TGG + chr6.1:33935961-33935980 MS.gene035466:intron 30.0%
!! TGAATTGCTTTGCTTGTTTT+TGG + chr6.1:33936316-33936335 MS.gene035466:intron 30.0%
!!! CTTCATTATTTTTGTTGGCA+AGG - chr6.1:33934146-33934165 None:intergenic 30.0%
AACAAAGTCACCACAAGTTT+GGG + chr6.1:33935801-33935820 MS.gene035466:intron 35.0%
AACACACGACAAAGAACAAA+AGG - chr6.1:33934603-33934622 None:intergenic 35.0%
AAGAACTGACGAATAAGCTT+TGG - chr6.1:33935286-33935305 None:intergenic 35.0%
AAGTTACTCAGAAACCTATG+GGG + chr6.1:33934718-33934737 MS.gene035466:CDS 35.0%
AATCCTCAGGTATGGAATTT+GGG + chr6.1:33934483-33934502 MS.gene035466:intron 35.0%
AGAATGGCATCAAAACTAGT+TGG + chr6.1:33934433-33934452 MS.gene035466:CDS 35.0%
AGGAGTTAAATAAGAGGGAA+AGG - chr6.1:33935507-33935526 None:intergenic 35.0%
ATAACAAGGAGTTCATCAGA+AGG - chr6.1:33934117-33934136 None:intergenic 35.0%
CATACGAGGAGTTAAATAAG+AGG - chr6.1:33935513-33935532 None:intergenic 35.0%
GAAGTTACTCAGAAACCTAT+GGG + chr6.1:33934717-33934736 MS.gene035466:CDS 35.0%
GAGTAAGACGCAATTCAAAT+GGG - chr6.1:33934227-33934246 None:intergenic 35.0%
GTATGTATTGCAACACATGA+AGG + chr6.1:33935528-33935547 MS.gene035466:intron 35.0%
TAACAAGGAGTTCATCAGAA+GGG - chr6.1:33934116-33934135 None:intergenic 35.0%
TGAAGCAGTAAAATTCCCTT+GGG - chr6.1:33934269-33934288 None:intergenic 35.0%
! ATGGATTAATTATGGGCCTT+TGG - chr6.1:33935314-33935333 None:intergenic 35.0%
! ATTGGATTTTGGATGCAGAA+GGG + chr6.1:33934324-33934343 MS.gene035466:CDS 35.0%
! CGTCAGTTCTTTTAATCCAA+AGG + chr6.1:33935295-33935314 MS.gene035466:intron 35.0%
! GATACTCAACGATTCTCTTT+TGG + chr6.1:33935424-33935443 MS.gene035466:intron 35.0%
! GGCAATGTAAGATTGGATTT+TGG + chr6.1:33934313-33934332 MS.gene035466:CDS 35.0%
!! ATTGAAAGCATTAGTTGCTC+TGG - chr6.1:33936406-33936425 None:intergenic 35.0%
!! CAATGGTTCTGACTTGATTA+AGG + chr6.1:33934179-33934198 MS.gene035466:CDS 35.0%
!! TTGAAAGCATTAGTTGCTCT+GGG - chr6.1:33936405-33936424 None:intergenic 35.0%
!!! ATCGTATGGTTTTGGTAGAA+TGG + chr6.1:33935725-33935744 MS.gene035466:intron 35.0%
!!! GCTTGTTTTTGGTGTTTATC+AGG + chr6.1:33936327-33936346 MS.gene035466:intron 35.0%
AGGACGTGATTTGAAGAATC+TGG - chr6.1:33935097-33935116 None:intergenic 40.0%
AGTAAAATTCCCTTGGGTGA+CGG - chr6.1:33934263-33934282 None:intergenic 40.0%
AGTTACTCAGAAACCTATGG+GGG + chr6.1:33934719-33934738 MS.gene035466:CDS 40.0%
ATAGGAAATTCCGCTCAGAA+TGG + chr6.1:33934417-33934436 MS.gene035466:CDS 40.0%
ATCGAGGAAATCACGAACAA+AGG - chr6.1:33934522-33934541 None:intergenic 40.0%
ATGGATCTGGATGTTGAGTT+TGG + chr6.1:33934639-33934658 MS.gene035466:CDS 40.0%
CAACAAAGTCACCACAAGTT+TGG + chr6.1:33935800-33935819 MS.gene035466:intron 40.0%
CAATCCTCAGGTATGGAATT+TGG + chr6.1:33934482-33934501 MS.gene035466:intron 40.0%
CAGATGAGGCAATGTAAGAT+TGG + chr6.1:33934306-33934325 MS.gene035466:CDS 40.0%
CATGTGTTGCAATACATACG+AGG - chr6.1:33935527-33935546 None:intergenic 40.0%
CATTGTCAAAAAGTGACGAC+AGG - chr6.1:33936245-33936264 None:intergenic 40.0%
CGAAGTTACTCAGAAACCTA+TGG + chr6.1:33934716-33934735 MS.gene035466:CDS 40.0%
GAGAGGAATATGGTGAAAGT+AGG + chr6.1:33935587-33935606 MS.gene035466:intron 40.0%
GCAATTCAAATGGGTCATAC+TGG - chr6.1:33934218-33934237 None:intergenic 40.0%
GCTGTTCGAGACATTATTGT+TGG + chr6.1:33934348-33934367 MS.gene035466:CDS 40.0%
GGAGTAAGACGCAATTCAAA+TGG - chr6.1:33934228-33934247 None:intergenic 40.0%
GTGAAGCAGTAAAATTCCCT+TGG - chr6.1:33934270-33934289 None:intergenic 40.0%
TCGAGGAAATCACGAACAAA+GGG - chr6.1:33934521-33934540 None:intergenic 40.0%
TGGCAAGGTAGTAGATAACA+AGG - chr6.1:33934131-33934150 None:intergenic 40.0%
TTGCTAACAATCCTCAGGTA+TGG + chr6.1:33934475-33934494 MS.gene035466:intron 40.0%
TTTCCCAAATTCCATACCTG+AGG - chr6.1:33934489-33934508 None:intergenic 40.0%
! GATTGGATTTTGGATGCAGA+AGG + chr6.1:33934323-33934342 MS.gene035466:CDS 40.0%
! TGTTGGAACTGATAACGTGT+TGG + chr6.1:33934365-33934384 MS.gene035466:CDS 40.0%
! TTGGATTTTGGATGCAGAAG+GGG + chr6.1:33934325-33934344 MS.gene035466:CDS 40.0%
! TTTGGAGCAGATGTTGATGA+AGG + chr6.1:33934657-33934676 MS.gene035466:CDS 40.0%
!! AGTTTTGATGCCATTCTGAG+CGG - chr6.1:33934430-33934449 None:intergenic 40.0%
!! CGAATAAGCTTTGGTGTCAA+CGG - chr6.1:33935277-33935296 None:intergenic 40.0%
!! TGAAAGCATTAGTTGCTCTG+GGG - chr6.1:33936404-33936423 None:intergenic 40.0%
AAGTCACCACAAGTTTGGGA+AGG + chr6.1:33935805-33935824 MS.gene035466:intron 45.0%
AGTCACCACAAGTTTGGGAA+GGG + chr6.1:33935806-33935825 MS.gene035466:intron 45.0%
CAAATGGGTCATACTGGTAC+AGG - chr6.1:33934212-33934231 None:intergenic 45.0%
CACTCTTGCTAACAATCCTC+AGG + chr6.1:33934470-33934489 MS.gene035466:CDS 45.0%
CCTCATCTGCTCATCAACTT+TGG - chr6.1:33934295-33934314 None:intergenic 45.0%
CGAGGAAATCACGAACAAAG+GGG - chr6.1:33934520-33934539 None:intergenic 45.0%
GTTTGGGAAGGGTTGTGAAT+TGG + chr6.1:33935817-33935836 MS.gene035466:intron 45.0%
TCAACATCCAGATCCATCTC+TGG - chr6.1:33934636-33934655 None:intergenic 45.0%
TGACGATGTTGTCTTGCACA+TGG + chr6.1:33936369-33936388 MS.gene035466:CDS 45.0%
! CTTTTTCTTCCGTCACCCAA+GGG + chr6.1:33934251-33934270 MS.gene035466:CDS 45.0%
! GGAACTGATAACGTGTTGGT+GGG + chr6.1:33934369-33934388 MS.gene035466:CDS 45.0%
! GTTGATGAAGGTGATGCTAC+TGG + chr6.1:33934669-33934688 MS.gene035466:CDS 45.0%
! TGGAACTGATAACGTGTTGG+TGG + chr6.1:33934368-33934387 MS.gene035466:CDS 45.0%
! TGGATTTTGGATGCAGAAGG+GGG + chr6.1:33934326-33934345 MS.gene035466:CDS 45.0%
!! CCAAAGTTGATGAGCAGATG+AGG + chr6.1:33934292-33934311 MS.gene035466:CDS 45.0%
!! AAATTTAAAGAAAAATAAAA+AGG - chr6.1:33935117-33935136 None:intergenic 5.0%
ACATCCAGATCCATCTCTGG+TGG - chr6.1:33934633-33934652 None:intergenic 50.0%
CACAACCCTTCCCAAACTTG+TGG - chr6.1:33935814-33935833 None:intergenic 50.0%
CATCCAGATCCATCTCTGGT+GGG - chr6.1:33934632-33934651 None:intergenic 50.0%
GAAGGTGATGCTACTGGAGA+TGG + chr6.1:33934675-33934694 MS.gene035466:CDS 50.0%
GAATTGCTTCCACCTGGTGT+TGG + chr6.1:33934084-33934103 MS.gene035466:CDS 50.0%
GGAAATCACGAACAAAGGGG+AGG - chr6.1:33934517-33934536 None:intergenic 50.0%
TCAACGAATCCGTTGACACC+TGG + chr6.1:33936097-33936116 MS.gene035466:intron 50.0%
TCAGAAACCTATGGGGGCTA+GGG + chr6.1:33934725-33934744 MS.gene035466:CDS 50.0%
! CCTTTTTCTTCCGTCACCCA+AGG + chr6.1:33934250-33934269 MS.gene035466:CDS 50.0%
!! CCTTGGGTGACGGAAGAAAA+AGG - chr6.1:33934253-33934272 None:intergenic 50.0%
!! GGGTGACGGAAGAAAAAGGA+CGG - chr6.1:33934249-33934268 None:intergenic 50.0%
!!! AGATTTTGAAGGCACCCTGC+AGG + chr6.1:33934800-33934819 MS.gene035466:CDS 50.0%
AAACCTATGGGGGCTAGGGA+TGG + chr6.1:33934729-33934748 MS.gene035466:CDS 55.0%
AACCTATGGGGGCTAGGGAT+GGG + chr6.1:33934730-33934749 MS.gene035466:CDS 55.0%
AGTCAGGATCCGTTGACACC+AGG - chr6.1:33936118-33936137 None:intergenic 55.0%
CCGTTGACACCAGGTGTCAA+CGG - chr6.1:33936109-33936128 None:intergenic 55.0%
CTCAGAAACCTATGGGGGCT+AGG + chr6.1:33934724-33934743 MS.gene035466:CDS 55.0%
GAAGGGATGAAGCCAACACC+AGG - chr6.1:33934099-33934118 None:intergenic 55.0%
GTTTGACTGCCCACCAGAGA+TGG + chr6.1:33934620-33934639 MS.gene035466:CDS 55.0%
! AGTGCTGCTGTTGAGCAACG+CGG + chr6.1:33934762-33934781 MS.gene035466:CDS 55.0%
! CCGTTGACACCTGGTGTCAA+CGG + chr6.1:33936106-33936125 MS.gene035466:intron 55.0%
ACCTATGGGGGCTAGGGATG+GGG + chr6.1:33934731-33934750 MS.gene035466:CDS 60.0%
CTGCCCACCAGAGATGGATC+TGG + chr6.1:33934626-33934645 MS.gene035466:CDS 60.0%
GGGATGAAGCCAACACCAGG+TGG - chr6.1:33934096-33934115 None:intergenic 60.0%
ATGGGGGCTAGGGATGGGGC+TGG + chr6.1:33934735-33934754 MS.gene035466:CDS 70.0%
GCCCCATCCCTAGCCCCCAT+AGG - chr6.1:33934735-33934754 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 33934081 33936465 33934081 ID=MS.gene035466
chr6.1 mRNA 33934081 33936465 33934081 ID=MS.gene035466.t1;Parent=MS.gene035466
chr6.1 exon 33934081 33934491 33934081 ID=MS.gene035466.t1.exon1;Parent=MS.gene035466.t1
chr6.1 CDS 33934081 33934491 33934081 ID=cds.MS.gene035466.t1;Parent=MS.gene035466.t1
chr6.1 exon 33934597 33934821 33934597 ID=MS.gene035466.t1.exon2;Parent=MS.gene035466.t1
chr6.1 CDS 33934597 33934821 33934597 ID=cds.MS.gene035466.t1;Parent=MS.gene035466.t1
chr6.1 exon 33936349 33936465 33936349 ID=MS.gene035466.t1.exon3;Parent=MS.gene035466.t1
chr6.1 CDS 33936349 33936465 33936349 ID=cds.MS.gene035466.t1;Parent=MS.gene035466.t1
Gene Sequence

>MS.gene035466

ATGGAATTGCTTCCACCTGGTGTTGGCTTCATCCCTTCTGATGAACTCCTTGTTATCTACTACCTTGCCAACAAAAATAATGAAGAGGTTCAAAATTTCAATGGTTCTGACTTGATTAAGGAGCTAAACCTGTACCAGTATGACCCATTTGAATTGCGTCTTACTCCGTCCTTTTTCTTCCGTCACCCAAGGGAATTTTACTGCTTCACTGCCAAAGTTGATGAGCAGATGAGGCAATGTAAGATTGGATTTTGGATGCAGAAGGGGGCTGTTCGAGACATTATTGTTGGAACTGATAACGTGTTGGTGGGAAAGAAGTCTCTTTTTATGTTCTATATAGGAAATTCCGCTCAGAATGGCATCAAAACTAGTTGGACTATGTACGAGTACACTCTTGCTAACAATCCTCAGGTATGGAATTTGGGAAAACTAACCTCCCCTTTGTTCGTGATTTCCTCGATGATTATTTAATGTATTAGCAACTACTAATTGTTTTTTTTTTAAATTATTATGCAGGCTCCTTTTGTTCTTTGTCGTGTGTTTGACTGCCCACCAGAGATGGATCTGGATGTTGAGTTTGGAGCAGATGTTGATGAAGGTGATGCTACTGGAGATGGCTCTGCTAACAAGCTGAACGAAGTTACTCAGAAACCTATGGGGGCTAGGGATGGGGCTGGTACTAGTGCTGCTGTTGAGCAACGCGGTGCATCAAATTTCACAGATTTTGAAGGCACCCTGCAGGTAAAATATATTCCATTTTGATCTCTCTTTATATTAACCAATTAATGTTGTTGATCTTATTTGCAACTGATTCTTTAAAATTTCGAAAATTTTACAACTTAATCCATTCATGTGATGTATTTTTTTATCGCACCAATCTTTGCTACTAAATGTAACATGTCAATTGTATGTATCACTCTGCACATGTATTTTTTGTTTTGTTTGTGTGTATAAATTAAGTGATAATTCTCAAAACATATAGGAATTTTGATTAGTTAATGTAGCTATTCTTACCAGATTCTTCAAATCACGTCCTTTTTATTTTTCTTTAAATTTTTTGCTATGATAAATTCATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATGAGTTAGGATCCGTTGACACCAAAGCTTATTCGTCAGTTCTTTTAATCCAAAGGCCCATAATTAATCCATGTTTCTTTTTCTTGTGTATCATTTCTCTCACCCACACACAATTATTGCTTTTAGTGATAAAAAAAACTCAAACTGAAAACATGGAGTTATGATACTCAACGATTCTCTTTTGGGAAAATTGTTTTTCTCTTCCCCCACTCCTATGAATATTATTCTTCTTCTTTGTATCTCCTTTCCCTCTTATTTAACTCCTCGTATGTATTGCAACACATGAAGGAAATTTATGTCAAGCTTATGATAACAATTACAATTGGAGAGGAATATGGTGAAAGTAGGAGATTGTTGATGAGTTTAATTTGCTTTTAATTTTATGTGTTTTACCATGATTGAATATGAATTAAAATAATCTTAGGATTTTATCGTTTTAAACGATTAGATGTCTACAAAATGAATCGTATGGTTTTGGTAGAATGGAAAACCTCATTACAATTTAATTTTATTATTTACAGCATAAACAATGAAATCTCAACAAAGTCACCACAAGTTTGGGAAGGGTTGTGAATTGGTTCAATTAGCATGTAGGAGAAGAGATCATTCTAAATAGTACTATCTTAAAAGTATTTTAATTATTCAGATATGGAGTATTTCAATTTTGTAGACAAGAGAACAAAGAGAATTATGATAGGATTATGTTTCCAATTTGGGTTTGGTAATCATTAAAAACAATAATAGTGTGTGATTGAAAAAAAGGATACGTAATAAATAAAAATGTGACACTATTATTAACCTTTGATTAAATAAAATCAACGGTTGAGATTTGGTGTCAACGAATCCGTTGACACCTGGTGTCAACGGATCCTGACTCTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTTTGTTGTTCCTGTCGTCACTTTTTGACAATGCAACTTTTCATTTCATATTATGGTAAAAACCAGAATGTATTGTTGAAGTATTGAATTGCTTTGCTTGTTTTTGGTGTTTATCAGGCGAGTTATGCTGCATTTCTTGACGATGTTGTCTTGCACATGGAAGCTATGACCCCAGAGCAACTAATGCTTTCAATTATGGATGACGATTTTCTTGAAATAGCCGATCTTGTGTAA
Protein sequence

>MS.gene035466.t1

MELLPPGVGFIPSDELLVIYYLANKNNEEVQNFNGSDLIKELNLYQYDPFELRLTPSFFFRHPREFYCFTAKVDEQMRQCKIGFWMQKGAVRDIIVGTDNVLVGKKSLFMFYIGNSAQNGIKTSWTMYEYTLANNPQAPFVLCRVFDCPPEMDLDVEFGADVDEGDATGDGSANKLNEVTQKPMGARDGAGTSAAVEQRGASNFTDFEGTLQASYAAFLDDVVLHMEAMTPEQLMLSIMDDDFLEIADLV