AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035472

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035472.t1 MTR_1g047060 53.125 96 36 2 111 206 3 89 8.33e-24 92.0
MS.gene035472.t1 MTR_4g094302 28.108 185 109 7 5 175 12 186 3.72e-15 72.0
MS.gene035472.t1 MTR_5g021710 31.818 154 85 6 5 145 9 155 4.17e-15 73.9
MS.gene035472.t1 MTR_4g101680 32.903 155 82 6 5 145 8 154 9.61e-15 73.2
MS.gene035472.t1 MTR_8g059170 35.766 137 70 7 5 133 15 141 1.72e-14 72.0
MS.gene035472.t1 MTR_7g105170 28.161 174 101 7 1 152 1 172 1.72e-14 71.2
MS.gene035472.t1 MTR_4g094302 30.709 127 80 4 5 131 12 130 2.11e-14 68.9
MS.gene035472.t1 MTR_2g080010 31.618 136 81 4 5 134 16 145 2.98e-14 71.6
MS.gene035472.t1 MTR_8g094580 34.074 135 74 7 5 133 8 133 5.74e-14 70.1
MS.gene035472.t1 MTR_2g079990 34.058 138 73 7 5 134 17 144 2.36e-13 68.9
MS.gene035472.t1 MTR_1g008740 32.468 154 85 7 5 145 28 175 2.39e-13 68.9
MS.gene035472.t1 MTR_1g008740 32.468 154 85 7 5 145 14 161 2.83e-13 68.9
MS.gene035472.t1 MTR_4g035590 30.460 174 100 8 5 165 9 174 3.36e-13 68.6
MS.gene035472.t1 MTR_2g079990 34.058 138 73 7 5 134 17 144 3.45e-13 68.6
MS.gene035472.t1 MTR_2g086880 26.316 209 109 10 6 189 20 208 4.37e-13 68.6
MS.gene035472.t1 MTR_2g086880 29.530 149 80 7 6 142 20 155 4.52e-13 68.6
MS.gene035472.t1 MTR_3g109340 31.169 154 86 7 5 145 23 169 7.66e-13 67.4
MS.gene035472.t1 MTR_3g096920 33.577 137 72 8 5 133 8 133 9.62e-13 66.6
MS.gene035472.t1 MTR_3g064580 30.000 160 82 8 5 145 7 155 1.11e-12 67.8
MS.gene035472.t1 MTR_3g096920 33.577 137 72 8 5 133 8 133 1.33e-12 66.6
MS.gene035472.t1 MTR_2g064090 30.657 137 81 6 5 134 7 136 8.61e-12 64.3
MS.gene035472.t1 MTR_1g069805 32.026 153 83 9 5 145 11 154 9.92e-12 63.5
MS.gene035472.t1 MTR_4g134460 35.075 134 70 7 6 131 56 180 1.10e-11 64.3
MS.gene035472.t1 MTR_6g011860 30.827 133 77 6 6 131 17 141 1.14e-11 64.3
MS.gene035472.t1 MTR_3g088110 32.353 136 75 6 5 133 8 133 1.52e-11 63.2
MS.gene035472.t1 MTR_5g040420 30.075 133 79 4 6 131 16 141 2.47e-11 63.2
MS.gene035472.t1 MTR_1g097300 32.593 135 73 6 6 131 21 146 2.59e-11 63.5
MS.gene035472.t1 MTR_8g023860 28.846 156 87 7 1 142 9 154 2.70e-11 63.2
MS.gene035472.t1 MTR_7g097090 32.680 153 82 9 5 145 11 154 2.73e-11 62.8
MS.gene035472.t1 MTR_8g023930 29.801 151 90 6 2 142 22 166 4.02e-11 62.8
MS.gene035472.t1 MTR_3g109340 29.412 153 89 7 5 145 23 168 4.24e-11 62.4
MS.gene035472.t1 MTR_4g108760 29.375 160 89 8 1 145 15 165 5.76e-11 62.0
MS.gene035472.t1 MTR_4g075980 29.530 149 90 6 6 145 17 159 6.00e-11 61.6
MS.gene035472.t1 MTR_8g093790 29.310 174 97 9 5 174 11 162 6.72e-11 60.1
MS.gene035472.t1 MTR_5g014300 26.452 155 95 6 5 144 7 157 6.73e-11 61.6
MS.gene035472.t1 MTR_2g068920 32.168 143 86 6 2 137 19 157 9.30e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035472.t1 AT4G36160 32.468 154 84 6 5 145 11 157 2.04e-16 78.2
MS.gene035472.t1 AT4G36160 32.468 154 84 6 5 145 11 157 2.04e-16 78.2
MS.gene035472.t1 AT4G36160 32.468 154 84 6 5 145 15 161 2.09e-16 78.2
MS.gene035472.t1 AT1G71930 29.677 155 93 5 5 148 10 159 8.04e-16 75.9
MS.gene035472.t1 AT1G71930 29.677 155 93 5 5 148 10 159 8.04e-16 75.9
MS.gene035472.t1 AT2G18060 31.818 154 85 6 5 145 10 156 1.03e-15 75.9
MS.gene035472.t1 AT2G18060 31.818 154 85 6 5 145 10 156 1.03e-15 75.9
MS.gene035472.t1 AT4G27410 31.677 161 85 8 5 150 15 165 1.74e-15 74.7
MS.gene035472.t1 AT4G27410 29.379 177 85 9 5 150 15 182 3.25e-14 71.2
MS.gene035472.t1 AT5G08790 29.108 213 118 10 5 196 8 208 1.00e-13 69.7
MS.gene035472.t1 AT4G17980 30.435 138 80 4 5 133 7 137 1.13e-13 69.3
MS.gene035472.t1 AT4G17980 30.435 138 80 4 5 133 7 137 1.43e-13 69.3
MS.gene035472.t1 AT3G15500 32.593 135 75 6 5 132 15 140 4.04e-13 68.2
MS.gene035472.t1 AT5G63790 36.765 136 69 8 5 133 51 176 4.39e-13 68.2
MS.gene035472.t1 AT5G63790 36.765 136 69 8 5 133 61 186 4.95e-13 67.8
MS.gene035472.t1 AT3G44290 25.874 143 93 5 6 142 16 151 5.34e-13 66.6
MS.gene035472.t1 AT3G44290 26.241 141 95 4 6 142 16 151 5.38e-13 67.8
MS.gene035472.t1 AT1G77450 36.496 137 68 7 5 133 11 136 5.46e-13 67.0
MS.gene035472.t1 AT1G52890 33.094 139 69 7 5 132 15 140 9.42e-13 67.0
MS.gene035472.t1 AT5G64530 33.077 130 69 5 5 131 4 118 1.41e-12 64.3
MS.gene035472.t1 AT1G01720 33.577 137 72 6 5 133 8 133 1.66e-12 66.2
MS.gene035472.t1 AT5G64530 27.604 192 106 5 5 175 4 183 2.91e-12 63.9
MS.gene035472.t1 AT5G17260 28.025 157 89 7 5 145 7 155 3.47e-12 65.9
MS.gene035472.t1 AT3G29035 32.090 134 75 6 5 131 25 149 4.97e-12 65.1
MS.gene035472.t1 AT3G03200 28.662 157 88 7 5 145 7 155 5.35e-12 65.5
MS.gene035472.t1 AT4G10350 31.210 157 84 8 5 145 10 158 7.11e-12 64.7
MS.gene035472.t1 AT4G28530 29.651 172 94 6 5 153 11 178 7.35e-12 64.7
MS.gene035472.t1 AT5G39610 30.573 157 84 8 5 145 21 168 7.71e-12 64.3
MS.gene035472.t1 AT5G04395 31.293 147 81 6 5 134 29 172 9.70e-12 62.4
MS.gene035472.t1 AT5G50820 34.074 135 73 7 5 134 18 141 9.76e-12 62.8
MS.gene035472.t1 AT1G26870 29.231 130 80 4 6 129 18 141 1.33e-11 64.3
MS.gene035472.t1 AT3G49530 26.994 163 91 9 1 145 10 162 1.39e-11 63.9
MS.gene035472.t1 AT3G49530 26.994 163 91 9 1 145 10 162 1.55e-11 63.9
MS.gene035472.t1 AT5G61430 29.697 165 92 8 1 150 14 169 1.88e-11 63.5
MS.gene035472.t1 AT5G64060 26.923 234 129 11 6 222 8 216 2.57e-11 63.2
MS.gene035472.t1 AT5G07680 30.000 160 88 8 1 145 15 165 4.90e-11 62.0
MS.gene035472.t1 AT1G33280 29.870 154 87 7 5 145 9 154 6.50e-11 61.6
MS.gene035472.t1 AT5G07680 30.000 160 88 8 1 145 1 151 6.52e-11 61.6

Find 55 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACTTATGCTTGCAATTA+TGG 0.158474 6.1:+34215674 MS.gene035472:CDS
ATGGATCTAGACTCTGAATT+TGG 0.204240 6.1:+34214495 MS.gene035472:CDS
GGCATTGTAAGACTGGATTT+TGG 0.240614 6.1:+34214165 MS.gene035472:CDS
CCATGGTTCTGACCGGATTA+AGG 0.270785 6.1:+34214031 MS.gene035472:CDS
CCTCCTCGGCTCCTCAGTTT+TGG 0.293397 6.1:-34214144 None:intergenic
TGGATCTAGACTCTGAATTT+GGG 0.333596 6.1:+34214496 MS.gene035472:CDS
CCTTGTGTGAGGGAAGAAAA+AGG 0.356880 6.1:-34214102 None:intergenic
TGTTGGAGCTGATGACGTTT+TGG 0.393392 6.1:+34214217 MS.gene035472:CDS
AAAACACAGCAAACAACAAA+AGG 0.433446 6.1:-34214456 None:intergenic
TGAAGGAACTCAGCAACTTA+AGG 0.444240 6.1:+34214578 MS.gene035472:CDS
ACAGTCTCAGCTCCTTAATC+CGG 0.483053 6.1:-34214043 None:intergenic
CCGAGGAGGCATTGTAAGAC+TGG 0.484776 6.1:+34214158 MS.gene035472:CDS
TGAGCAAGATGTAATTCAAA+AGG 0.487325 6.1:-34214077 None:intergenic
ACTCAGCAACTTAAGGAGGC+TGG 0.487489 6.1:+34214585 MS.gene035472:CDS
CTCAGCAACTTAAGGAGGCT+GGG 0.496847 6.1:+34214586 MS.gene035472:CDS
GTTGGTGTTGGTGTTGAGCA+AGG 0.501020 6.1:+34214627 MS.gene035472:CDS
GAGTCTAGATCCATCCCTCT+AGG 0.505901 6.1:-34214486 None:intergenic
GGCTACTCAAGCCACTCCTC+AGG 0.507680 6.1:+34214668 MS.gene035472:CDS
TCTAGTGCAGGTGTTGGTGT+TGG 0.508728 6.1:+34214615 MS.gene035472:CDS
GAAGAGGTTCAGAATTTCCA+TGG 0.526667 6.1:+34214014 MS.gene035472:CDS
AGAACGCGATCAAAACTAGT+TGG 0.526855 6.1:+34214285 MS.gene035472:CDS
GCTGTTAGAGACATTACTGT+TGG 0.528259 6.1:+34214200 MS.gene035472:CDS
CACTCTTGTTAACAATCCTC+AGG 0.528444 6.1:+34214322 MS.gene035472:CDS
AGAATTTCCATGGTTCTGAC+CGG 0.533129 6.1:+34214024 MS.gene035472:CDS
ACCTTCATCGACATCAGATA+AGG 0.533255 6.1:-34214520 None:intergenic
TTGTTCATCAGAAGGGAAGA+AGG 0.533280 6.1:-34213961 None:intergenic
TCTGATGACAATGCGAATGA+AGG 0.540561 6.1:+34214561 MS.gene035472:CDS
GCAATGGATTTGCCTCCACC+TGG 0.541606 6.1:+34213933 None:intergenic
CCAGTCTTACAATGCCTCCT+CGG 0.543084 6.1:-34214158 None:intergenic
CCACATGGAAGCTATGACGC+CGG 0.546830 6.1:+34215650 MS.gene035472:CDS
ATTGCAAGCATAAGTTGCTC+CGG 0.553025 6.1:-34215669 None:intergenic
AGGAGTGGCTTGAGTAGCCA+TGG 0.558328 6.1:-34214664 None:intergenic
AAGAAGGCAACACCAGGTGG+AGG 0.559720 6.1:-34213945 None:intergenic
CTAACAAGTTGTTCATCAGA+AGG 0.560217 6.1:-34213969 None:intergenic
GCTGGGACTAGTTCTAGTGC+AGG 0.570005 6.1:+34214603 MS.gene035472:CDS
AGTCTAGATCCATCCCTCTA+GGG 0.570873 6.1:-34214485 None:intergenic
AGGTGATGCATCGCATTCCA+TGG 0.574376 6.1:+34214647 MS.gene035472:CDS
CAGTAAAACTCCCTTGTGTG+AGG 0.577220 6.1:-34214113 None:intergenic
CCTTAATCCGGTCAGAACCA+TGG 0.580530 6.1:-34214031 None:intergenic
TAACAAGTTGTTCATCAGAA+GGG 0.581916 6.1:-34213968 None:intergenic
ACTAGTTCTAGTGCAGGTGT+TGG 0.583083 6.1:+34214609 MS.gene035472:CDS
GAAGGGAAGAAGGCAACACC+AGG 0.588550 6.1:-34213951 None:intergenic
GCCTTATCTGATGTCGATGA+AGG 0.589295 6.1:+34214519 MS.gene035472:CDS
AGTAAAACTCCCTTGTGTGA+GGG 0.603789 6.1:-34214112 None:intergenic
GTAATTCAAAAGGATCATAC+CGG 0.608058 6.1:-34214067 None:intergenic
TTAAGGAGCTGAGACTGTAC+CGG 0.611881 6.1:+34214048 MS.gene035472:CDS
GGGAAGAAGGCAACACCAGG+TGG 0.613301 6.1:-34213948 None:intergenic
CTGCCAAAACTGAGGAGCCG+AGG 0.619650 6.1:+34214141 MS.gene035472:CDS
CCGGCGTCATAGCTTCCATG+TGG 0.629672 6.1:-34215650 None:intergenic
TGACTATGTTGTGTCCCACA+TGG 0.631632 6.1:+34215635 MS.gene035472:CDS
GGATCTAGACTCTGAATTTG+GGG 0.633562 6.1:+34214497 MS.gene035472:CDS
CCAAAACTGAGGAGCCGAGG+AGG 0.634705 6.1:+34214144 MS.gene035472:CDS
CGGCGTCATAGCTTCCATGT+GGG 0.637767 6.1:-34215649 None:intergenic
CTGCTTCACTGCCAAAACTG+AGG 0.647730 6.1:+34214133 MS.gene035472:CDS
AGGAACTCAGCAACTTAAGG+AGG 0.651240 6.1:+34214581 MS.gene035472:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAATATAAATAATT+GGG + chr6.1:34214427-34214446 MS.gene035472:intron 0.0%
!!! TTTTTTTTAATATAAATAAT+TGG + chr6.1:34214426-34214445 MS.gene035472:intron 0.0%
!! AAACTTAAAGAAAAATAAAA+AGG - chr6.1:34215391-34215410 None:intergenic 10.0%
!! TACATATAATAAACAAAAAC+AGG - chr6.1:34214796-34214815 None:intergenic 15.0%
!!! ATGTTTTTTTTTTTCCTTTT+TGG + chr6.1:34215279-34215298 MS.gene035472:intron 15.0%
!!! ATTTTTCTTTAAGTTTTTTG+TGG + chr6.1:34215395-34215414 MS.gene035472:intron 15.0%
!! GTTTGATATTGATGTTTATT+AGG + chr6.1:34215593-34215612 MS.gene035472:intron 20.0%
!! TATTCAAAACCTTTATGTAT+TGG + chr6.1:34215000-34215019 MS.gene035472:intron 20.0%
!! TCGATGATTAATTTATGTAT+TGG + chr6.1:34214389-34214408 MS.gene035472:intron 20.0%
!! TTTAATATAAATAATTGGGC+AGG + chr6.1:34214431-34214450 MS.gene035472:intron 20.0%
!!! ATTGAAATATATTTTACCTG+AGG - chr6.1:34214687-34214706 None:intergenic 20.0%
!!! CATAATTTTTGATACAATTG+TGG - chr6.1:34215115-34215134 None:intergenic 20.0%
!!! CATCTTTTCATGTAATTTTA+TGG + chr6.1:34215532-34215551 MS.gene035472:intron 20.0%
!!! GATTAATTTATGTATTGGTA+AGG + chr6.1:34214394-34214413 MS.gene035472:intron 20.0%
!!! GTTTTGAATAACAACAAATA+TGG - chr6.1:34214990-34215009 None:intergenic 20.0%
!!! TTAATTTGTTACATGTTTTG+AGG - chr6.1:34215327-34215346 None:intergenic 20.0%
! ATATGTATTTCACTTTCTGT+TGG + chr6.1:34214809-34214828 MS.gene035472:intron 25.0%
! CATAAATAATCCAAACACAA+TGG - chr6.1:34215061-34215080 None:intergenic 25.0%
! CATGAAAATCATTGATATTG+TGG - chr6.1:34214959-34214940 None:intergenic 25.0%
! GAAAAACTACCAATACATAA+AGG - chr6.1:34215012-34215031 None:intergenic 25.0%
! TCGATGAAATAAAGAACATA+CGG - chr6.1:34214373-34214392 None:intergenic 25.0%
! TTAATTTCTACACACCAAAA+AGG - chr6.1:34215296-34215315 None:intergenic 25.0%
!! AATAAAAAGGACATGTTTTG+AGG - chr6.1:34215378-34215397 None:intergenic 25.0%
!! CTTTTCATGTAATTTTATGG+TGG + chr6.1:34215535-34215554 MS.gene035472:intron 25.0%
!! TCTCTCTTCATTTTCTATAT+AGG + chr6.1:34214251-34214270 MS.gene035472:CDS 25.0%
!!! TTATGTATTGGTAGTTTTTC+TGG + chr6.1:34215012-34215031 MS.gene035472:intron 25.0%
AAAACACAGCAAACAACAAA+AGG - chr6.1:34214459-34214478 None:intergenic 30.0%
AACTCAATTCATTCATGTGA+TGG + chr6.1:34215206-34215225 MS.gene035472:intron 30.0%
ACAATTGTGGATGTAGAATT+CGG - chr6.1:34215102-34215121 None:intergenic 30.0%
GTAATTCAAAAGGATCATAC+CGG - chr6.1:34214070-34214089 None:intergenic 30.0%
TAACAAGTTGTTCATCAGAA+GGG - chr6.1:34213971-34213990 None:intergenic 30.0%
TGAGCAAGATGTAATTCAAA+AGG - chr6.1:34214080-34214099 None:intergenic 30.0%
TGTAAAATTCAGTTGCAAAG+AGG - chr6.1:34215187-34215206 None:intergenic 30.0%
TGTGACCATGATTTAAAAGT+TGG - chr6.1:34214904-34214923 None:intergenic 30.0%
! AAGCATTGTAAAATTGTTGC+CGG - chr6.1:34214736-34214755 None:intergenic 30.0%
! AATATATTTTACCTGAGGAG+TGG - chr6.1:34214682-34214701 None:intergenic 30.0%
! CATGACCAACTTTTAAATCA+TGG + chr6.1:34214896-34214915 MS.gene035472:intron 30.0%
!!! AACCTCTTCATTGTTTTTGT+TGG - chr6.1:34214003-34214022 None:intergenic 30.0%
!!! CTCGATATTTATTTTGTCAC+CGG + chr6.1:34214714-34214733 MS.gene035472:intron 30.0%
AATCATGGTCACACTAATTG+TGG + chr6.1:34214911-34214930 MS.gene035472:intron 35.0%
AGAGGATCAACAACATTAAC+AGG - chr6.1:34215169-34215188 None:intergenic 35.0%
ATATTGTGGCTGACATATGA+TGG - chr6.1:34214858-34214877 None:intergenic 35.0%
ATGGATCTAGACTCTGAATT+TGG + chr6.1:34214495-34214514 MS.gene035472:CDS 35.0%
CTAACAAGTTGTTCATCAGA+AGG - chr6.1:34213972-34213991 None:intergenic 35.0%
GCAACTTATGCTTGCAATTA+TGG + chr6.1:34215674-34215693 MS.gene035472:CDS 35.0%
TGCCAACAAAAACAATGAAG+AGG + chr6.1:34213998-34214017 MS.gene035472:CDS 35.0%
TGGATCTAGACTCTGAATTT+GGG + chr6.1:34214496-34214515 MS.gene035472:CDS 35.0%
TGGATGTAGAATTCGGTAAT+TGG - chr6.1:34215095-34215114 None:intergenic 35.0%
! AATCCTCAGGTATGTCATTT+GGG + chr6.1:34214335-34214354 MS.gene035472:intron 35.0%
!!! CTTCATTGTTTTTGTTGGCA+AGG - chr6.1:34213998-34214017 None:intergenic 35.0%
ACCTTCATCGACATCAGATA+AGG - chr6.1:34214523-34214542 None:intergenic 40.0%
AGAACGCGATCAAAACTAGT+TGG + chr6.1:34214285-34214304 MS.gene035472:CDS 40.0%
AGTAAAACTCCCTTGTGTGA+GGG - chr6.1:34214115-34214134 None:intergenic 40.0%
ATTGCAAGCATAAGTTGCTC+CGG - chr6.1:34215672-34215691 None:intergenic 40.0%
CAATCCTCAGGTATGTCATT+TGG + chr6.1:34214334-34214353 MS.gene035472:intron 40.0%
CACTCTTGTTAACAATCCTC+AGG + chr6.1:34214322-34214341 MS.gene035472:CDS 40.0%
CCAAACACAATGGAGCTATT+TGG - chr6.1:34215051-34215070 None:intergenic 40.0%
CCAAATAGCTCCATTGTGTT+TGG + chr6.1:34215048-34215067 MS.gene035472:intron 40.0%
GAAGAGGTTCAGAATTTCCA+TGG + chr6.1:34214014-34214033 MS.gene035472:CDS 40.0%
GCTGTTAGAGACATTACTGT+TGG + chr6.1:34214200-34214219 MS.gene035472:CDS 40.0%
GGATCTAGACTCTGAATTTG+GGG + chr6.1:34214497-34214516 MS.gene035472:CDS 40.0%
TCTGATGACAATGCGAATGA+AGG + chr6.1:34214561-34214580 MS.gene035472:CDS 40.0%
TGAAGGAACTCAGCAACTTA+AGG + chr6.1:34214578-34214597 MS.gene035472:CDS 40.0%
TTGTTCATCAGAAGGGAAGA+AGG - chr6.1:34213964-34213983 None:intergenic 40.0%
TTTCCCAAATGACATACCTG+AGG - chr6.1:34214341-34214360 None:intergenic 40.0%
! ACTGGATTTTGGATGCAGAA+GGG + chr6.1:34214176-34214195 MS.gene035472:CDS 40.0%
! CTTTTTCTTCCCTCACACAA+GGG + chr6.1:34214103-34214122 MS.gene035472:CDS 40.0%
! GGCATTGTAAGACTGGATTT+TGG + chr6.1:34214165-34214184 MS.gene035472:CDS 40.0%
!! AGAATTTCCATGGTTCTGAC+CGG + chr6.1:34214024-34214043 MS.gene035472:CDS 40.0%
!!! AGGACATGTTTTGAGGAATC+TGG - chr6.1:34215371-34215390 None:intergenic 40.0%
!!! TAGTTTTGATCGCGTTCTGA+GGG - chr6.1:34214283-34214302 None:intergenic 40.0%
ACAGTCTCAGCTCCTTAATC+CGG - chr6.1:34214046-34214065 None:intergenic 45.0%
AGGAACTCAGCAACTTAAGG+AGG + chr6.1:34214581-34214600 MS.gene035472:CDS 45.0%
AGTCTAGATCCATCCCTCTA+GGG - chr6.1:34214488-34214507 None:intergenic 45.0%
CAGTAAAACTCCCTTGTGTG+AGG - chr6.1:34214116-34214135 None:intergenic 45.0%
CCTTGTGTGAGGGAAGAAAA+AGG - chr6.1:34214105-34214124 None:intergenic 45.0%
GCCTTATCTGATGTCGATGA+AGG + chr6.1:34214519-34214538 MS.gene035472:CDS 45.0%
TGACTATGTTGTGTCCCACA+TGG + chr6.1:34215635-34215654 MS.gene035472:CDS 45.0%
TTAAGGAGCTGAGACTGTAC+CGG + chr6.1:34214048-34214067 MS.gene035472:CDS 45.0%
TTGTGGCTGACATATGATGG+AGG - chr6.1:34214855-34214874 None:intergenic 45.0%
! CCTTTTTCTTCCCTCACACA+AGG + chr6.1:34214102-34214121 MS.gene035472:CDS 45.0%
! GACTGGATTTTGGATGCAGA+AGG + chr6.1:34214175-34214194 MS.gene035472:CDS 45.0%
!! ACTAGTTCTAGTGCAGGTGT+TGG + chr6.1:34214609-34214628 MS.gene035472:CDS 45.0%
!! AGTTTTGATCGCGTTCTGAG+GGG - chr6.1:34214282-34214301 None:intergenic 45.0%
!! GTGTTTTTGACTGCCCTAGA+GGG + chr6.1:34214472-34214491 MS.gene035472:CDS 45.0%
!! TGTGTTTTTGACTGCCCTAG+AGG + chr6.1:34214471-34214490 MS.gene035472:CDS 45.0%
!! TGTTGGAGCTGATGACGTTT+TGG + chr6.1:34214217-34214236 MS.gene035472:CDS 45.0%
!!! CTAGTTTTGATCGCGTTCTG+AGG - chr6.1:34214284-34214303 None:intergenic 45.0%
ACTCAGCAACTTAAGGAGGC+TGG + chr6.1:34214585-34214604 MS.gene035472:CDS 50.0%
AGGTGATGCATCGCATTCCA+TGG + chr6.1:34214647-34214666 MS.gene035472:CDS 50.0%
CCAGTCTTACAATGCCTCCT+CGG - chr6.1:34214161-34214180 None:intergenic 50.0%
CCTTAATCCGGTCAGAACCA+TGG - chr6.1:34214034-34214053 None:intergenic 50.0%
CTCAGCAACTTAAGGAGGCT+GGG + chr6.1:34214586-34214605 MS.gene035472:CDS 50.0%
CTGCTTCACTGCCAAAACTG+AGG + chr6.1:34214133-34214152 MS.gene035472:CDS 50.0%
GAGTCTAGATCCATCCCTCT+AGG - chr6.1:34214489-34214508 None:intergenic 50.0%
!! CCATGGTTCTGACCGGATTA+AGG + chr6.1:34214031-34214050 MS.gene035472:CDS 50.0%
!! GGAGCTGATGACGTTTTGGT+GGG + chr6.1:34214221-34214240 MS.gene035472:CDS 50.0%
!! GTTGGTGTTGGTGTTGAGCA+AGG + chr6.1:34214627-34214646 MS.gene035472:CDS 50.0%
!! TCTAGTGCAGGTGTTGGTGT+TGG + chr6.1:34214615-34214634 MS.gene035472:CDS 50.0%
!! TGGAGCTGATGACGTTTTGG+TGG + chr6.1:34214220-34214239 MS.gene035472:CDS 50.0%
!! TTTTGACTGCCCTAGAGGGA+TGG + chr6.1:34214476-34214495 MS.gene035472:CDS 50.0%
AAGAAGGCAACACCAGGTGG+AGG - chr6.1:34213948-34213967 None:intergenic 55.0%
AGGAGTGGCTTGAGTAGCCA+TGG - chr6.1:34214667-34214686 None:intergenic 55.0%
CCACATGGAAGCTATGACGC+CGG + chr6.1:34215650-34215669 MS.gene035472:CDS 55.0%
CGGCGTCATAGCTTCCATGT+GGG - chr6.1:34215652-34215671 None:intergenic 55.0%
GAAGGGAAGAAGGCAACACC+AGG - chr6.1:34213954-34213973 None:intergenic 55.0%
!! CCGAGGAGGCATTGTAAGAC+TGG + chr6.1:34214158-34214177 MS.gene035472:CDS 55.0%
!! GCTGGGACTAGTTCTAGTGC+AGG + chr6.1:34214603-34214622 MS.gene035472:CDS 55.0%
CCAAAACTGAGGAGCCGAGG+AGG + chr6.1:34214144-34214163 MS.gene035472:CDS 60.0%
CCGGCGTCATAGCTTCCATG+TGG - chr6.1:34215653-34215672 None:intergenic 60.0%
CTGCCAAAACTGAGGAGCCG+AGG + chr6.1:34214141-34214160 MS.gene035472:CDS 60.0%
GGCTACTCAAGCCACTCCTC+AGG + chr6.1:34214668-34214687 MS.gene035472:CDS 60.0%
GGGAAGAAGGCAACACCAGG+TGG - chr6.1:34213951-34213970 None:intergenic 60.0%
! CCTCCTCGGCTCCTCAGTTT+TGG - chr6.1:34214147-34214166 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 34213936 34215716 34213936 ID=MS.gene035472
chr6.1 mRNA 34213936 34215716 34213936 ID=MS.gene035472.t1;Parent=MS.gene035472
chr6.1 exon 34213936 34214343 34213936 ID=MS.gene035472.t1.exon1;Parent=MS.gene035472.t1
chr6.1 CDS 34213936 34214343 34213936 ID=cds.MS.gene035472.t1;Parent=MS.gene035472.t1
chr6.1 exon 34214453 34214689 34214453 ID=MS.gene035472.t1.exon2;Parent=MS.gene035472.t1
chr6.1 CDS 34214453 34214689 34214453 ID=cds.MS.gene035472.t1;Parent=MS.gene035472.t1
chr6.1 exon 34215615 34215716 34215615 ID=MS.gene035472.t1.exon3;Parent=MS.gene035472.t1
chr6.1 CDS 34215615 34215716 34215615 ID=cds.MS.gene035472.t1;Parent=MS.gene035472.t1
Gene Sequence

>MS.gene035472

ATGGATTTGCCTCCACCTGGTGTTGCCTTCTTCCCTTCTGATGAACAACTTGTTAGCTACCTTGCCAACAAAAACAATGAAGAGGTTCAGAATTTCCATGGTTCTGACCGGATTAAGGAGCTGAGACTGTACCGGTATGATCCTTTTGAATTACATCTTGCTCAGTCCTTTTTCTTCCCTCACACAAGGGAGTTTTACTGCTTCACTGCCAAAACTGAGGAGCCGAGGAGGCATTGTAAGACTGGATTTTGGATGCAGAAGGGAGCTGTTAGAGACATTACTGTTGGAGCTGATGACGTTTTGGTGGGAAAGAAGTCTCTCTTCATTTTCTATATAGGAAATTCCCCTCAGAACGCGATCAAAACTAGTTGGACTATGTACGAGTACACTCTTGTTAACAATCCTCAGGTATGTCATTTGGGAAAACTAACTTTCCGTATGTTCTTTATTTCATCGATGATTAATTTATGTATTGGTAAGGACTAACTATTTTTTTTTAATATAAATAATTGGGCAGGCTCCTTTTGTTGTTTGCTGTGTTTTTGACTGCCCTAGAGGGATGGATCTAGACTCTGAATTTGGGGCCTTATCTGATGTCGATGAAGGTGATGTTGCTGATGACGACTCTGATGACAATGCGAATGAAGGAACTCAGCAACTTAAGGAGGCTGGGACTAGTTCTAGTGCAGGTGTTGGTGTTGGTGTTGAGCAAGGTGATGCATCGCATTCCATGGCTACTCAAGCCACTCCTCAGGTAAAATATATTTCAATTTGATCTCTCGATATTTATTTTGTCACCGGCAACAATTTTACAATGCTTAAATTTGTTTGAAATTTAAATTCAAAATTGTTTTTTGCCTGTTTTTGTTTATTATATGTATTTCACTTTCTGTTGGACATAAGTTTTAATAAAATACCTCCATCATATGTCAGCCACAATATCAATGATTTTCATGTATGCATGACCAACTTTTAAATCATGGTCACACTAATTGTGGTAATTGCATGAAAATCATTGATATTGCAAATAATAATGATCAAGCTGCATTAGCCATATTTGTTGTTATTCAAAACCTTTATGTATTGGTAGTTTTTCTGGTAGATGTTTGAATCCAAATAGCTCCATTGTGTTTGGATTATTTATGTTTTGCAGTTGCCAATTACCGAATTCTACATCCACAATTGTATCAAAAATTATGTTTAATTGTTGAAGTCAACATTTGCAGTTAACCTGTTAATGTTGTTGATCCTCTTTGCAACTGAATTTTACAACTCAATTCATTCATGTGATGGTATTTTTTTTTATTTTTTTTGATCAATTTTATTGAATGTATCACTCTGACATGTTTTTTTTTTTCCTTTTTGGTGTGTAGAAATTAAGTGATGATCCTCAAAACATGTAACAAATTAATAGTTAACATAGCTATTCTAACCAGATTCCTCAAAACATGTCCTTTTTATTTTTCTTTAAGTTTTTTGTGGTATTAAATTTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATGATCATCATCATCATCATCATCATCACTTTTTGACAAAGCATCTTTTCATGTAATTTTATGGTGGAAAAATAAAGTGTTGCTGAAATATTGAATTTCTTTGTTTGATATTGATGTTTATTAGGAGAGTTATACTGCATTTCTTGACTATGTTGTGTCCCACATGGAAGCTATGACGCCGGAGCAACTTATGCTTGCAATTATGGATGAGAAAGATTTTATTTAA
Protein sequence

>MS.gene035472.t1

MDLPPPGVAFFPSDEQLVSYLANKNNEEVQNFHGSDRIKELRLYRYDPFELHLAQSFFFPHTREFYCFTAKTEEPRRHCKTGFWMQKGAVRDITVGADDVLVGKKSLFIFYIGNSPQNAIKTSWTMYEYTLVNNPQAPFVVCCVFDCPRGMDLDSEFGALSDVDEGDVADDDSDDNANEGTQQLKEAGTSSSAGVGVGVEQGDASHSMATQATPQESYTAFLDYVVSHMEAMTPEQLMLAIMDEKDFI