AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035475

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035475.t1 MTR_1g047060 52.874 87 32 1 111 188 3 89 4.21e-22 87.0
MS.gene035475.t1 MTR_7g105170 28.161 174 101 7 1 152 1 172 1.07e-14 71.6
MS.gene035475.t1 MTR_4g094302 27.568 185 110 7 5 175 12 186 1.09e-14 70.1
MS.gene035475.t1 MTR_5g021710 31.169 154 86 6 5 145 9 155 1.54e-14 72.0
MS.gene035475.t1 MTR_8g059170 35.766 137 70 7 5 133 15 141 3.51e-14 70.9
MS.gene035475.t1 MTR_2g080010 31.618 136 81 4 5 134 16 145 5.06e-14 70.5
MS.gene035475.t1 MTR_4g094302 29.921 127 81 4 5 131 12 130 7.50e-14 67.4
MS.gene035475.t1 MTR_8g094580 34.074 135 74 7 5 133 8 133 1.20e-13 68.9
MS.gene035475.t1 MTR_4g035590 30.460 174 100 8 5 165 9 174 1.79e-13 68.9
MS.gene035475.t1 MTR_6g477900 28.977 176 93 8 5 165 7 165 2.19e-13 67.4
MS.gene035475.t1 MTR_3g064580 30.625 160 81 8 5 145 7 155 2.26e-13 69.3
MS.gene035475.t1 MTR_2g079990 34.058 138 73 7 5 134 17 144 3.89e-13 68.2
MS.gene035475.t1 MTR_2g079990 34.058 138 73 7 5 134 17 144 5.59e-13 67.8
MS.gene035475.t1 MTR_2g086880 29.530 149 80 7 6 142 20 155 6.46e-13 67.8
MS.gene035475.t1 MTR_2g086880 29.530 149 80 7 6 142 20 155 6.70e-13 67.8
MS.gene035475.t1 MTR_5g076850 31.111 135 77 6 5 131 7 133 8.90e-13 67.4
MS.gene035475.t1 MTR_3g096920 33.577 137 72 8 5 133 8 133 1.25e-12 66.2
MS.gene035475.t1 MTR_3g096920 33.577 137 72 8 5 133 8 133 1.54e-12 66.2
MS.gene035475.t1 MTR_8g023900 29.870 154 88 7 2 142 10 156 3.94e-12 65.5
MS.gene035475.t1 MTR_8g467490 30.827 133 78 5 6 131 16 141 4.11e-12 65.1
MS.gene035475.t1 MTR_8g023860 30.769 156 84 8 1 142 9 154 5.80e-12 64.7
MS.gene035475.t1 MTR_1g097300 33.333 135 72 6 6 131 21 146 7.79e-12 64.7
MS.gene035475.t1 MTR_1g069805 31.579 152 85 8 5 145 11 154 1.01e-11 63.5
MS.gene035475.t1 MTR_8g093790 29.885 174 96 9 5 174 11 162 1.13e-11 62.0
MS.gene035475.t1 MTR_8g023930 30.464 151 89 6 2 142 22 166 1.21e-11 63.9
MS.gene035475.t1 MTR_2g062730 30.769 156 85 7 5 145 11 158 1.99e-11 63.2
MS.gene035475.t1 MTR_6g011860 32.090 134 74 7 6 131 17 141 2.79e-11 62.8
MS.gene035475.t1 MTR_7g097090 32.237 152 84 8 5 145 11 154 2.98e-11 62.0
MS.gene035475.t1 MTR_3g088110 32.353 136 75 6 5 133 8 133 2.98e-11 62.0
MS.gene035475.t1 MTR_4g134460 35.075 134 70 7 6 131 56 180 4.07e-11 62.4
MS.gene035475.t1 MTR_5g014300 24.378 201 122 7 5 194 7 188 4.52e-11 62.0
MS.gene035475.t1 MTR_5g040420 31.343 134 76 5 6 131 16 141 5.25e-11 62.0
MS.gene035475.t1 MTR_7g005280 30.667 150 88 7 5 145 16 158 5.59e-11 61.2
MS.gene035475.t1 MTR_2g068880 32.168 143 86 6 2 137 20 158 6.93e-11 61.2
MS.gene035475.t1 MTR_8g023840 31.333 150 83 8 5 142 12 153 8.94e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035475.t1 AT4G36160 32.237 152 87 5 5 145 15 161 8.40e-16 75.9
MS.gene035475.t1 AT4G36160 32.237 152 87 5 5 145 11 157 9.91e-16 75.5
MS.gene035475.t1 AT4G36160 32.237 152 87 5 5 145 11 157 9.91e-16 75.5
MS.gene035475.t1 AT4G27410 31.677 161 85 8 5 150 15 165 2.99e-15 73.6
MS.gene035475.t1 AT1G71930 29.032 155 94 5 5 148 10 159 3.47e-15 73.6
MS.gene035475.t1 AT1G71930 29.032 155 94 5 5 148 10 159 3.47e-15 73.6
MS.gene035475.t1 AT2G18060 31.169 154 86 6 5 145 10 156 4.71e-15 73.6
MS.gene035475.t1 AT2G18060 31.169 154 86 6 5 145 10 156 4.71e-15 73.6
MS.gene035475.t1 AT1G76420 32.075 159 80 8 5 146 23 170 1.69e-14 72.0
MS.gene035475.t1 AT4G27410 29.379 177 85 9 5 150 15 182 5.25e-14 70.1
MS.gene035475.t1 AT3G17730 29.487 156 87 8 5 145 7 154 2.13e-13 67.8
MS.gene035475.t1 AT4G17980 30.435 138 80 4 5 133 7 137 2.18e-13 67.8
MS.gene035475.t1 AT4G17980 30.435 138 80 4 5 133 7 137 2.53e-13 68.2
MS.gene035475.t1 AT5G08790 35.294 136 71 7 5 133 8 133 4.28e-13 67.4
MS.gene035475.t1 AT5G17260 28.662 157 88 7 5 145 7 155 5.14e-13 68.2
MS.gene035475.t1 AT1G77450 36.496 137 68 7 5 133 11 136 6.00e-13 66.6
MS.gene035475.t1 AT5G63790 36.765 136 69 8 5 133 51 176 6.26e-13 67.0
MS.gene035475.t1 AT3G15500 32.593 135 75 6 5 132 15 140 6.71e-13 67.0
MS.gene035475.t1 AT5G63790 36.765 136 69 8 5 133 61 186 6.84e-13 67.0
MS.gene035475.t1 AT3G44290 26.241 141 95 4 6 142 16 151 9.23e-13 67.0
MS.gene035475.t1 AT1G32510 30.070 143 81 5 5 134 7 143 9.93e-13 66.2
MS.gene035475.t1 AT3G44290 26.241 141 95 4 6 142 16 151 1.02e-12 65.5
MS.gene035475.t1 AT3G10490 30.968 155 85 7 6 145 29 176 1.99e-12 65.1
MS.gene035475.t1 AT1G52890 33.088 136 73 7 5 132 15 140 2.00e-12 65.9
MS.gene035475.t1 AT4G10350 31.847 157 83 8 5 145 10 158 2.00e-12 65.9
MS.gene035475.t1 AT1G54330 28.758 153 89 7 5 145 7 151 2.50e-12 65.5
MS.gene035475.t1 AT1G01720 33.577 137 72 6 5 133 8 133 3.16e-12 65.1
MS.gene035475.t1 AT4G28530 29.651 172 94 6 5 153 11 178 5.49e-12 64.7
MS.gene035475.t1 AT5G39610 30.573 157 84 8 5 145 21 168 5.61e-12 64.3
MS.gene035475.t1 AT3G10490 30.968 155 85 7 6 145 29 176 5.95e-12 64.7
MS.gene035475.t1 AT3G29035 32.090 134 75 6 5 131 25 149 9.83e-12 63.9
MS.gene035475.t1 AT5G50820 33.333 135 74 6 5 134 18 141 1.27e-11 62.0
MS.gene035475.t1 AT1G33280 30.519 154 86 7 5 145 9 154 1.43e-11 63.2
MS.gene035475.t1 AT5G13180 30.000 150 90 6 5 145 15 158 1.66e-11 62.4
MS.gene035475.t1 AT5G61430 29.697 165 92 8 1 150 14 169 2.51e-11 62.8
MS.gene035475.t1 AT5G04395 30.000 150 85 6 5 137 29 175 2.69e-11 60.8
MS.gene035475.t1 AT2G02450 31.410 156 85 8 6 148 53 199 3.86e-11 62.4
MS.gene035475.t1 AT2G02450 31.410 156 85 8 6 148 53 199 5.06e-11 62.0
MS.gene035475.t1 AT3G10480 30.519 154 86 7 6 145 29 175 6.36e-11 62.0
MS.gene035475.t1 AT5G07680 30.000 160 88 8 1 145 15 165 9.35e-11 60.8

Find 49 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACTTATGCTTGCAATTA+TGG 0.158474 6.1:+34141414 MS.gene035475:CDS
ATGGATCTAGACTCTGAATT+TGG 0.204240 6.1:+34140281 MS.gene035475:CDS
GGCATTGTAAGACTGGATTT+TGG 0.240614 6.1:+34139949 MS.gene035475:CDS
CCATGGTTCTAACCGGATTA+AGG 0.244298 6.1:+34139815 MS.gene035475:CDS
CCTCCTCGGCTCCTCAGTTT+TGG 0.293397 6.1:-34139928 None:intergenic
TGGATCTAGACTCTGAATTT+GGG 0.333596 6.1:+34140282 MS.gene035475:CDS
CCTTGTGTGAGGGAAGAAAA+AGG 0.356880 6.1:-34139886 None:intergenic
TGTTGGAGCTGATGACGTTT+TGG 0.393392 6.1:+34140001 MS.gene035475:CDS
AAAACACAGCAAACAACAAA+AGG 0.433446 6.1:-34140242 None:intergenic
AGAATTTCCATGGTTCTAAC+CGG 0.433731 6.1:+34139808 MS.gene035475:CDS
CCGAGGAGGCATTGTAAGAC+TGG 0.484776 6.1:+34139942 MS.gene035475:CDS
TGAGCAAGATGTAATTCAAA+AGG 0.487325 6.1:-34139861 None:intergenic
ACAGTCTCAGCTCCTTAATC+CGG 0.488240 6.1:-34139827 None:intergenic
GAGTCTAGATCCATCCCTCT+AGG 0.505901 6.1:-34140272 None:intergenic
GGCTACTCAAGCCACTCCTC+AGG 0.507680 6.1:+34140400 MS.gene035475:CDS
ATAACGCGATCAAAACTAGT+TGG 0.523204 6.1:+34140069 MS.gene035475:CDS
GAAGAGGTTCAGAATTTCCA+TGG 0.526667 6.1:+34139798 MS.gene035475:CDS
GCTGTTAGAGACATTACTGT+TGG 0.528259 6.1:+34139984 MS.gene035475:CDS
CACTCTTGTTAACAATCCTC+AGG 0.528444 6.1:+34140106 MS.gene035475:CDS
ACCTTCATCGACATCAGATA+AGG 0.533255 6.1:-34140306 None:intergenic
TTGTTCATCAGAAGGGAAGA+AGG 0.533280 6.1:-34139745 None:intergenic
TCTGATGACAAGGCGAATGA+AGG 0.534755 6.1:+34140347 MS.gene035475:CDS
GCAATGGATTTGCCTCCACC+TGG 0.541606 6.1:+34139717 None:intergenic
CCAGTCTTACAATGCCTCCT+CGG 0.543084 6.1:-34139942 None:intergenic
CCACATGGAAGCTATGACGC+CGG 0.546830 6.1:+34141390 MS.gene035475:CDS
ATTGCAAGCATAAGTTGCTC+CGG 0.553025 6.1:-34141409 None:intergenic
AGGAGTGGCTTGAGTAGCCA+TGG 0.558328 6.1:-34140396 None:intergenic
CCTTAATCCGGTTAGAACCA+TGG 0.559295 6.1:-34139815 None:intergenic
AAGAAGGCAACACCAGGTGG+AGG 0.559720 6.1:-34139729 None:intergenic
CTAACAAGTTGTTCATCAGA+AGG 0.560217 6.1:-34139753 None:intergenic
AGTCTAGATCCATCCCTCTA+GGG 0.570873 6.1:-34140271 None:intergenic
AGGTGATGCATCGCATTCCA+TGG 0.574376 6.1:+34140379 MS.gene035475:CDS
CAGTAAAACTCCCTTGTGTG+AGG 0.577220 6.1:-34139897 None:intergenic
TAACAAGTTGTTCATCAGAA+GGG 0.581916 6.1:-34139752 None:intergenic
GAAGGGAAGAAGGCAACACC+AGG 0.588550 6.1:-34139735 None:intergenic
GCCTTATCTGATGTCGATGA+AGG 0.589295 6.1:+34140305 MS.gene035475:CDS
AGTAAAACTCCCTTGTGTGA+GGG 0.603789 6.1:-34139896 None:intergenic
GTAATTCAAAAGGATCATAC+CGG 0.608058 6.1:-34139851 None:intergenic
GCGAATGAAGGTGTTGAGCA+AGG 0.608609 6.1:+34140359 MS.gene035475:CDS
TTAAGGAGCTGAGACTGTAC+CGG 0.611881 6.1:+34139832 MS.gene035475:CDS
GGGAAGAAGGCAACACCAGG+TGG 0.613301 6.1:-34139732 None:intergenic
CTGCCAAAACTGAGGAGCCG+AGG 0.619650 6.1:+34139925 MS.gene035475:CDS
CCGGCGTCATAGCTTCCATG+TGG 0.629672 6.1:-34141390 None:intergenic
TGACTATGTTGTGTCCCACA+TGG 0.631632 6.1:+34141375 MS.gene035475:CDS
GGATCTAGACTCTGAATTTG+GGG 0.633562 6.1:+34140283 MS.gene035475:CDS
CCAAAACTGAGGAGCCGAGG+AGG 0.634705 6.1:+34139928 MS.gene035475:CDS
CGGCGTCATAGCTTCCATGT+GGG 0.637767 6.1:-34141389 None:intergenic
CTGCTTCACTGCCAAAACTG+AGG 0.647730 6.1:+34139917 MS.gene035475:CDS
TGATGACGACTCTGATGACA+AGG 0.678157 6.1:+34140337 MS.gene035475:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAATATAAATAATT+GGG + chr6.1:34140213-34140232 MS.gene035475:intron 0.0%
!!! TTTTTTTTAATATAAATAAT+TGG + chr6.1:34140212-34140231 MS.gene035475:intron 0.0%
!! AAACTTAAAGAAAAATAAAA+AGG - chr6.1:34141122-34141141 None:intergenic 10.0%
!! TACATATAATAAACAAAAAC+AGG - chr6.1:34140528-34140547 None:intergenic 15.0%
!!! ATTTTTCTTTAAGTTTTTTG+TGG + chr6.1:34141126-34141145 MS.gene035475:intron 15.0%
!! GTTTGATATTGATGTTTATT+AGG + chr6.1:34141333-34141352 MS.gene035475:intron 20.0%
!! TATTCAAAACCTTTATGTAT+TGG + chr6.1:34140732-34140751 MS.gene035475:intron 20.0%
!! TCGATGATTAATTTATGTAT+TGG + chr6.1:34140173-34140192 MS.gene035475:intron 20.0%
!! TTTAATATAAATAATTGGGC+AGG + chr6.1:34140217-34140236 MS.gene035475:intron 20.0%
!!! ATTGAAATATATTTTACCTG+AGG - chr6.1:34140419-34140438 None:intergenic 20.0%
!!! CATCTTTTCATGTAATTTTA+TGG + chr6.1:34141272-34141291 MS.gene035475:intron 20.0%
!!! CATGTTTTTTTTTTCCTTTT+TGG + chr6.1:34141010-34141029 MS.gene035475:intron 20.0%
!!! GATTAATTTATGTATTGGTA+AGG + chr6.1:34140178-34140197 MS.gene035475:intron 20.0%
!!! GTTTTGAATAACAACAAATA+TGG - chr6.1:34140722-34140741 None:intergenic 20.0%
!!! TTAATTTGTTACATGTTTTG+AGG - chr6.1:34141058-34141077 None:intergenic 20.0%
! ACAATTGTTGATGTAGAATT+CGG - chr6.1:34140834-34140853 None:intergenic 25.0%
! ATATGTATTTCACTTTCTGT+TGG + chr6.1:34140541-34140560 MS.gene035475:intron 25.0%
! CATAAATAATCCAAACACAA+TGG - chr6.1:34140793-34140812 None:intergenic 25.0%
! CATGAAAATCATTGATATTG+TGG - chr6.1:34140691-34140672 None:intergenic 25.0%
! GAAAAACTACCAATACATAA+AGG - chr6.1:34140744-34140763 None:intergenic 25.0%
! TCGATGAAATAAAGAACATA+CGG - chr6.1:34140157-34140176 None:intergenic 25.0%
! TTAATTTCTACACACCAAAA+AGG - chr6.1:34141027-34141046 None:intergenic 25.0%
!! AATAAAAAGGACATGTTTTG+AGG - chr6.1:34141109-34141128 None:intergenic 25.0%
!! CTTTTCATGTAATTTTATGG+TGG + chr6.1:34141275-34141294 MS.gene035475:intron 25.0%
!! TCTCTCTTCATTTTCTATAT+AGG + chr6.1:34140035-34140054 MS.gene035475:CDS 25.0%
!!! TTATGTATTGGTAGTTTTTC+TGG + chr6.1:34140744-34140763 MS.gene035475:intron 25.0%
AAAACACAGCAAACAACAAA+AGG - chr6.1:34140245-34140264 None:intergenic 30.0%
AACTCAATTCATTCATGTGA+TGG + chr6.1:34140938-34140957 MS.gene035475:intron 30.0%
GTAATTCAAAAGGATCATAC+CGG - chr6.1:34139854-34139873 None:intergenic 30.0%
TAACAAGTTGTTCATCAGAA+GGG - chr6.1:34139755-34139774 None:intergenic 30.0%
TGAGCAAGATGTAATTCAAA+AGG - chr6.1:34139864-34139883 None:intergenic 30.0%
TGTAAAATTCAGTTGCAAAG+AGG - chr6.1:34140919-34140938 None:intergenic 30.0%
TGTGACCATGATTTAAAAGT+TGG - chr6.1:34140636-34140655 None:intergenic 30.0%
TTGATGTAGAATTCGGTAAT+TGG - chr6.1:34140827-34140846 None:intergenic 30.0%
! AAGCATTGTAAAATTGTTGC+CGG - chr6.1:34140468-34140487 None:intergenic 30.0%
! AATATATTTTACCTGAGGAG+TGG - chr6.1:34140414-34140433 None:intergenic 30.0%
! CATGACCAACTTTTAAATCA+TGG + chr6.1:34140628-34140647 MS.gene035475:intron 30.0%
!!! AACCTCTTCATTGTTTTTGT+TGG - chr6.1:34139787-34139806 None:intergenic 30.0%
!!! CTCGATATTTATTTTGTCAC+CGG + chr6.1:34140446-34140465 MS.gene035475:intron 30.0%
AATCATGGTCACACTAATTG+TGG + chr6.1:34140643-34140662 MS.gene035475:intron 35.0%
AGAGGATCAACAACATTAAC+AGG - chr6.1:34140901-34140920 None:intergenic 35.0%
ATAACGCGATCAAAACTAGT+TGG + chr6.1:34140069-34140088 MS.gene035475:CDS 35.0%
ATATTGTGGCTGACATATGA+TGG - chr6.1:34140590-34140609 None:intergenic 35.0%
ATGGATCTAGACTCTGAATT+TGG + chr6.1:34140281-34140300 MS.gene035475:CDS 35.0%
CTAACAAGTTGTTCATCAGA+AGG - chr6.1:34139756-34139775 None:intergenic 35.0%
GCAACTTATGCTTGCAATTA+TGG + chr6.1:34141414-34141433 MS.gene035475:CDS 35.0%
TGCCAACAAAAACAATGAAG+AGG + chr6.1:34139782-34139801 MS.gene035475:CDS 35.0%
TGGATCTAGACTCTGAATTT+GGG + chr6.1:34140282-34140301 MS.gene035475:CDS 35.0%
! AATCCTCAGGTATGTCATTT+GGG + chr6.1:34140119-34140138 MS.gene035475:intron 35.0%
!! AGAATTTCCATGGTTCTAAC+CGG + chr6.1:34139808-34139827 MS.gene035475:CDS 35.0%
!!! CTTCATTGTTTTTGTTGGCA+AGG - chr6.1:34139782-34139801 None:intergenic 35.0%
!!! TAGTTTTGATCGCGTTATGA+GGG - chr6.1:34140067-34140086 None:intergenic 35.0%
ACCTTCATCGACATCAGATA+AGG - chr6.1:34140309-34140328 None:intergenic 40.0%
AGTAAAACTCCCTTGTGTGA+GGG - chr6.1:34139899-34139918 None:intergenic 40.0%
ATTGCAAGCATAAGTTGCTC+CGG - chr6.1:34141412-34141431 None:intergenic 40.0%
CAATCCTCAGGTATGTCATT+TGG + chr6.1:34140118-34140137 MS.gene035475:intron 40.0%
CACTCTTGTTAACAATCCTC+AGG + chr6.1:34140106-34140125 MS.gene035475:CDS 40.0%
CCAAACACAATGGAGCTATT+TGG - chr6.1:34140783-34140802 None:intergenic 40.0%
CCAAATAGCTCCATTGTGTT+TGG + chr6.1:34140780-34140799 MS.gene035475:intron 40.0%
GAAGAGGTTCAGAATTTCCA+TGG + chr6.1:34139798-34139817 MS.gene035475:CDS 40.0%
GCTGTTAGAGACATTACTGT+TGG + chr6.1:34139984-34140003 MS.gene035475:CDS 40.0%
GGATCTAGACTCTGAATTTG+GGG + chr6.1:34140283-34140302 MS.gene035475:CDS 40.0%
TTGTTCATCAGAAGGGAAGA+AGG - chr6.1:34139748-34139767 None:intergenic 40.0%
TTTCCCAAATGACATACCTG+AGG - chr6.1:34140125-34140144 None:intergenic 40.0%
! ACTGGATTTTGGATGCAGAA+GGG + chr6.1:34139960-34139979 MS.gene035475:CDS 40.0%
! CTTTTTCTTCCCTCACACAA+GGG + chr6.1:34139887-34139906 MS.gene035475:CDS 40.0%
! GGCATTGTAAGACTGGATTT+TGG + chr6.1:34139949-34139968 MS.gene035475:CDS 40.0%
!! AGTTTTGATCGCGTTATGAG+GGG - chr6.1:34140066-34140085 None:intergenic 40.0%
!!! AGGACATGTTTTGAGGAATC+TGG - chr6.1:34141102-34141121 None:intergenic 40.0%
!!! CTAGTTTTGATCGCGTTATG+AGG - chr6.1:34140068-34140087 None:intergenic 40.0%
ACAGTCTCAGCTCCTTAATC+CGG - chr6.1:34139830-34139849 None:intergenic 45.0%
AGTCTAGATCCATCCCTCTA+GGG - chr6.1:34140274-34140293 None:intergenic 45.0%
CAGTAAAACTCCCTTGTGTG+AGG - chr6.1:34139900-34139919 None:intergenic 45.0%
CCTTAATCCGGTTAGAACCA+TGG - chr6.1:34139818-34139837 None:intergenic 45.0%
CCTTGTGTGAGGGAAGAAAA+AGG - chr6.1:34139889-34139908 None:intergenic 45.0%
GCCTTATCTGATGTCGATGA+AGG + chr6.1:34140305-34140324 MS.gene035475:CDS 45.0%
TCTGATGACAAGGCGAATGA+AGG + chr6.1:34140347-34140366 MS.gene035475:CDS 45.0%
TGACTATGTTGTGTCCCACA+TGG + chr6.1:34141375-34141394 MS.gene035475:CDS 45.0%
TTAAGGAGCTGAGACTGTAC+CGG + chr6.1:34139832-34139851 MS.gene035475:CDS 45.0%
TTGTGGCTGACATATGATGG+AGG - chr6.1:34140587-34140606 None:intergenic 45.0%
! CCTTTTTCTTCCCTCACACA+AGG + chr6.1:34139886-34139905 MS.gene035475:CDS 45.0%
! GACTGGATTTTGGATGCAGA+AGG + chr6.1:34139959-34139978 MS.gene035475:CDS 45.0%
! TGATGACGACTCTGATGACA+AGG + chr6.1:34140337-34140356 MS.gene035475:CDS 45.0%
!! CCATGGTTCTAACCGGATTA+AGG + chr6.1:34139815-34139834 MS.gene035475:CDS 45.0%
!! GTGTTTTTGACTGCCCTAGA+GGG + chr6.1:34140258-34140277 MS.gene035475:CDS 45.0%
!! TGTGTTTTTGACTGCCCTAG+AGG + chr6.1:34140257-34140276 MS.gene035475:CDS 45.0%
!! TGTTGGAGCTGATGACGTTT+TGG + chr6.1:34140001-34140020 MS.gene035475:CDS 45.0%
AGGTGATGCATCGCATTCCA+TGG + chr6.1:34140379-34140398 MS.gene035475:CDS 50.0%
CCAGTCTTACAATGCCTCCT+CGG - chr6.1:34139945-34139964 None:intergenic 50.0%
CTGCTTCACTGCCAAAACTG+AGG + chr6.1:34139917-34139936 MS.gene035475:CDS 50.0%
GAGTCTAGATCCATCCCTCT+AGG - chr6.1:34140275-34140294 None:intergenic 50.0%
!! GCGAATGAAGGTGTTGAGCA+AGG + chr6.1:34140359-34140378 MS.gene035475:CDS 50.0%
!! GGAGCTGATGACGTTTTGGT+GGG + chr6.1:34140005-34140024 MS.gene035475:CDS 50.0%
!! TGGAGCTGATGACGTTTTGG+TGG + chr6.1:34140004-34140023 MS.gene035475:CDS 50.0%
!! TTTTGACTGCCCTAGAGGGA+TGG + chr6.1:34140262-34140281 MS.gene035475:CDS 50.0%
AAGAAGGCAACACCAGGTGG+AGG - chr6.1:34139732-34139751 None:intergenic 55.0%
AGGAGTGGCTTGAGTAGCCA+TGG - chr6.1:34140399-34140418 None:intergenic 55.0%
CCACATGGAAGCTATGACGC+CGG + chr6.1:34141390-34141409 MS.gene035475:CDS 55.0%
CGGCGTCATAGCTTCCATGT+GGG - chr6.1:34141392-34141411 None:intergenic 55.0%
GAAGGGAAGAAGGCAACACC+AGG - chr6.1:34139738-34139757 None:intergenic 55.0%
!! CCGAGGAGGCATTGTAAGAC+TGG + chr6.1:34139942-34139961 MS.gene035475:CDS 55.0%
CCAAAACTGAGGAGCCGAGG+AGG + chr6.1:34139928-34139947 MS.gene035475:CDS 60.0%
CCGGCGTCATAGCTTCCATG+TGG - chr6.1:34141393-34141412 None:intergenic 60.0%
CTGCCAAAACTGAGGAGCCG+AGG + chr6.1:34139925-34139944 MS.gene035475:CDS 60.0%
GGCTACTCAAGCCACTCCTC+AGG + chr6.1:34140400-34140419 MS.gene035475:CDS 60.0%
GGGAAGAAGGCAACACCAGG+TGG - chr6.1:34139735-34139754 None:intergenic 60.0%
! CCTCCTCGGCTCCTCAGTTT+TGG - chr6.1:34139931-34139950 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 34139720 34141456 34139720 ID=MS.gene035475
chr6.1 mRNA 34139720 34141456 34139720 ID=MS.gene035475.t1;Parent=MS.gene035475
chr6.1 exon 34139720 34140127 34139720 ID=MS.gene035475.t1.exon1;Parent=MS.gene035475.t1
chr6.1 CDS 34139720 34140127 34139720 ID=cds.MS.gene035475.t1;Parent=MS.gene035475.t1
chr6.1 exon 34140239 34140421 34140239 ID=MS.gene035475.t1.exon2;Parent=MS.gene035475.t1
chr6.1 CDS 34140239 34140421 34140239 ID=cds.MS.gene035475.t1;Parent=MS.gene035475.t1
chr6.1 exon 34141355 34141456 34141355 ID=MS.gene035475.t1.exon3;Parent=MS.gene035475.t1
chr6.1 CDS 34141355 34141456 34141355 ID=cds.MS.gene035475.t1;Parent=MS.gene035475.t1
Gene Sequence

>MS.gene035475

ATGGATTTGCCTCCACCTGGTGTTGCCTTCTTCCCTTCTGATGAACAACTTGTTAGCTACCTTGCCAACAAAAACAATGAAGAGGTTCAGAATTTCCATGGTTCTAACCGGATTAAGGAGCTGAGACTGTACCGGTATGATCCTTTTGAATTACATCTTGCTCAGTCCTTTTTCTTCCCTCACACAAGGGAGTTTTACTGCTTCACTGCCAAAACTGAGGAGCCGAGGAGGCATTGTAAGACTGGATTTTGGATGCAGAAGGGAGCTGTTAGAGACATTACTGTTGGAGCTGATGACGTTTTGGTGGGAAAGAAGTCTCTCTTCATTTTCTATATAGGAAATTCCCCTCATAACGCGATCAAAACTAGTTGGACTATGTACGAGTACACTCTTGTTAACAATCCTCAGGTATGTCATTTGGGAAAACTAACTTTCCGTATGTTCTTTATTTCATCGATGATTAATTTATGTATTGGTAAGGACTAACTATTTTTTTTTTTAATATAAATAATTGGGCAGGCTCCTTTTGTTGTTTGCTGTGTTTTTGACTGCCCTAGAGGGATGGATCTAGACTCTGAATTTGGGGCCTTATCTGATGTCGATGAAGGTGATGTTGCTGATGACGACTCTGATGACAAGGCGAATGAAGGTGTTGAGCAAGGTGATGCATCGCATTCCATGGCTACTCAAGCCACTCCTCAGGTAAAATATATTTCAATTTGATCTCTCGATATTTATTTTGTCACCGGCAACAATTTTACAATGCTTAAATTTGTTTGAAATTTAAATTCAAAATTGTTTTTTGCCTGTTTTTGTTTATTATATGTATTTCACTTTCTGTTGGACATAAGTTTTAATAAAATACCTCCATCATATGTCAGCCACAATATCAATGATTTTCATGTATGCATGACCAACTTTTAAATCATGGTCACACTAATTGTGGTAATTGCATGAAAATCATTGATATTGCAAATAATAATGATCAAGCTGCATTAGCCATATTTGTTGTTATTCAAAACCTTTATGTATTGGTAGTTTTTCTGGTAGATGTTTGAATCCAAATAGCTCCATTGTGTTTGGATTATTTATGTTTTGCAGTTGCCAATTACCGAATTCTACATCAACAATTGTATCAAAAATTATGTTTAATTGTTGAAGTCAACATTTGCAGTTAACCTGTTAATGTTGTTGATCCTCTTTGCAACTGAATTTTACAACTCAATTCATTCATGTGATGGTATTTTTTTTATTTTTTTTTGATCAATTTTATTGAATGTATCACTCTGACATGTTTTTTTTTTCCTTTTTGGTGTGTAGAAATTAAGTGATGATCCTCAAAACATGTAACAAATTAATAGTTAACATAGCTATTCTAACCAGATTCCTCAAAACATGTCCTTTTTATTTTTCTTTAAGTTTTTTGTGGTATTAAATTTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATGATCATCATCATCATCATCATCATCACTTTTTGACAAAGCATCTTTTCATGTAATTTTATGGTGGAAAAATAAAGTGTTGCTGAAATATTGAATTTCTTTGTTTGATATTGATGTTTATTAGGCGAGTTATACTGCATTTCTTGACTATGTTGTGTCCCACATGGAAGCTATGACGCCGGAGCAACTTATGCTTGCAATTATGGATGAGAAAGATTTTATTTAA
Protein sequence

>MS.gene035475.t1

MDLPPPGVAFFPSDEQLVSYLANKNNEEVQNFHGSNRIKELRLYRYDPFELHLAQSFFFPHTREFYCFTAKTEEPRRHCKTGFWMQKGAVRDITVGADDVLVGKKSLFIFYIGNSPHNAIKTSWTMYEYTLVNNPQAPFVVCCVFDCPRGMDLDSEFGALSDVDEGDVADDDSDDKANEGVEQGDASHSMATQATPQASYTAFLDYVVSHMEAMTPEQLMLAIMDEKDFI