AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035555

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035555.t1 MTR_4g131600 91.534 567 37 1 1 556 24 590 0.0 1028
MS.gene035555.t1 MTR_4g131570 71.503 579 139 4 1 556 21 596 0.0 804
MS.gene035555.t1 MTR_4g131580 70.526 570 149 4 1 551 21 590 0.0 778
MS.gene035555.t1 MTR_2g450070 40.465 645 270 21 1 547 22 650 3.65e-119 367
MS.gene035555.t1 MTR_2g450070 40.465 645 270 21 1 547 22 650 1.09e-118 366
MS.gene035555.t1 MTR_3g106220 52.038 319 137 6 1 303 12 330 1.66e-89 289
MS.gene035555.t1 MTR_1g013170 48.640 331 139 6 1 303 9 336 3.29e-89 284
MS.gene035555.t1 MTR_1g032570 54.472 246 102 5 1 237 15 259 1.93e-80 265
MS.gene035555.t1 MTR_1g013180 48.265 317 136 6 1 292 160 473 7.21e-78 259
MS.gene035555.t1 MTR_4g121020 44.258 357 166 9 1 330 35 385 3.66e-77 257
MS.gene035555.t1 MTR_4g121020 44.258 357 166 9 1 330 35 385 3.98e-77 257
MS.gene035555.t1 MTR_3g086100 53.846 247 101 5 1 238 17 259 4.25e-77 254
MS.gene035555.t1 MTR_7g117705 46.488 299 153 4 1 292 9 307 2.40e-74 244
MS.gene035555.t1 MTR_8g079940 48.855 262 118 5 1 257 29 279 6.56e-74 245
MS.gene035555.t1 MTR_2g034960 53.361 238 110 1 1 237 33 270 3.17e-73 245
MS.gene035555.t1 MTR_1g032570 54.974 191 76 5 56 237 1 190 2.26e-58 204
MS.gene035555.t1 MTR_3g102590 49.569 232 69 3 1 231 12 196 1.80e-57 192
MS.gene035555.t1 MTR_3g102590 49.561 228 67 3 1 227 12 192 1.04e-54 183
MS.gene035555.t1 MTR_3g086100 51.042 192 81 5 56 238 1 188 4.74e-51 182
MS.gene035555.t1 MTR_4g021760 37.391 230 121 4 1 230 15 221 2.62e-46 165
MS.gene035555.t1 MTR_4g021790 36.508 252 131 4 1 252 14 236 9.89e-44 157
MS.gene035555.t1 MTR_3g102600 62.500 120 45 0 1 120 12 131 1.20e-43 154
MS.gene035555.t1 MTR_3g102600 60.377 53 21 0 177 229 131 183 4.22e-15 74.3
MS.gene035555.t1 MTR_3g102600 62.500 120 45 0 1 120 12 131 1.43e-43 155
MS.gene035555.t1 MTR_3g102600 42.056 107 45 2 177 283 131 220 7.66e-18 83.2
MS.gene035555.t1 MTR_4g021855 37.179 234 124 3 1 234 14 224 7.90e-42 152
MS.gene035555.t1 MTR_4g021845 37.719 228 117 5 1 227 14 217 8.31e-41 147
MS.gene035555.t1 MTR_6g045327 35.341 249 128 6 2 241 19 243 2.28e-40 151
MS.gene035555.t1 MTR_4g121020 37.943 282 142 9 76 330 6 281 1.64e-39 152
MS.gene035555.t1 MTR_0450s0040 36.364 242 136 6 1 242 8 231 1.80e-39 147
MS.gene035555.t1 MTR_1g013160 48.344 151 73 2 59 204 1 151 2.91e-36 132
MS.gene035555.t1 MTR_8g105600 34.914 232 146 3 1 231 13 240 3.38e-31 128
MS.gene035555.t1 MTR_3g102600 63.415 82 30 0 39 120 1 82 1.22e-30 117
MS.gene035555.t1 MTR_3g102600 60.377 53 21 0 177 229 82 134 2.63e-15 73.6
MS.gene035555.t1 MTR_7g026400 35.096 208 124 3 27 234 17 213 2.67e-30 125
MS.gene035555.t1 MTR_3g102600 63.415 82 30 0 39 120 1 82 7.39e-30 115
MS.gene035555.t1 MTR_3g102600 42.056 107 45 2 177 283 82 171 1.14e-17 81.3
MS.gene035555.t1 MTR_5g055260 33.333 231 102 8 1 230 14 193 1.04e-28 115
MS.gene035555.t1 MTR_8g032710 29.921 254 151 7 1 230 21 271 9.19e-27 114
MS.gene035555.t1 MTR_3g092780 32.979 188 105 5 1 174 56 236 3.48e-24 107
MS.gene035555.t1 MTR_3g092780 32.979 188 105 5 1 174 56 236 3.85e-24 107
MS.gene035555.t1 MTR_3g092780 32.240 183 112 4 1 174 56 235 2.15e-23 104
MS.gene035555.t1 MTR_3g092780 32.240 183 112 4 1 174 56 235 2.25e-23 104
MS.gene035555.t1 MTR_4g023980 31.875 160 85 3 43 201 35 171 3.54e-22 94.4
MS.gene035555.t1 MTR_8g093040 28.632 234 129 3 1 234 18 213 1.13e-21 95.5
MS.gene035555.t1 MTR_7g118260 36.154 130 74 2 1 124 14 140 3.50e-21 97.8
MS.gene035555.t1 MTR_7g118260 36.800 125 70 2 1 119 14 135 7.82e-21 96.7
MS.gene035555.t1 MTR_4g061360 37.705 122 73 1 1 119 89 210 1.29e-19 93.2
MS.gene035555.t1 MTR_4g064730 52.381 84 37 2 154 234 104 187 1.29e-18 87.4
MS.gene035555.t1 MTR_1g067110 34.426 122 77 1 1 119 85 206 2.11e-18 89.4
MS.gene035555.t1 MTR_6g016850 32.215 149 98 2 1 147 13 160 2.36e-18 83.2
MS.gene035555.t1 MTR_1g067110 34.426 122 77 1 1 119 85 206 2.37e-18 89.4
MS.gene035555.t1 MTR_8g024260 33.600 125 80 1 3 124 38 162 9.10e-18 87.4
MS.gene035555.t1 MTR_3g037390 31.707 123 81 1 1 120 24 146 3.10e-17 85.5
MS.gene035555.t1 MTR_7g089010 58.621 58 24 0 177 234 90 147 2.39e-16 80.5
MS.gene035555.t1 MTR_4g108880 30.081 123 83 1 1 120 33 155 2.87e-16 82.4
MS.gene035555.t1 MTR_5g014040 37.500 120 65 1 131 240 264 383 1.45e-15 80.1
MS.gene035555.t1 MTR_5g014040 35.714 126 75 3 1 124 18 139 5.76e-13 72.0
MS.gene035555.t1 MTR_4g098870 48.750 80 31 1 171 240 303 382 1.99e-15 79.7
MS.gene035555.t1 MTR_4g098870 33.333 120 76 2 1 119 18 134 1.78e-11 67.0
MS.gene035555.t1 MTR_4g098870 48.750 80 31 1 171 240 323 402 2.05e-15 79.7
MS.gene035555.t1 MTR_4g098870 33.333 120 76 2 1 119 38 154 2.28e-11 67.0
MS.gene035555.t1 MTR_4g098870 48.750 80 31 1 171 240 303 382 2.30e-15 79.3
MS.gene035555.t1 MTR_4g098870 33.333 120 76 2 1 119 18 134 1.09e-11 67.8
MS.gene035555.t1 MTR_4g098870 48.750 80 31 1 171 240 342 421 2.47e-15 79.3
MS.gene035555.t1 MTR_4g098870 33.333 120 76 2 1 119 57 173 1.57e-11 67.4
MS.gene035555.t1 MTR_4g098870 48.750 80 31 1 171 240 323 402 2.49e-15 79.3
MS.gene035555.t1 MTR_4g098870 33.333 120 76 2 1 119 38 154 1.48e-11 67.4
MS.gene035555.t1 MTR_7g115530 62.500 56 20 1 176 230 216 271 6.36e-14 73.2
MS.gene035555.t1 MTR_5g014040 32.484 157 84 4 131 273 224 372 1.00e-13 73.9
MS.gene035555.t1 MTR_7g115530 62.500 56 20 1 176 230 216 271 1.18e-13 72.8
MS.gene035555.t1 MTR_7g115530 62.500 56 20 1 176 230 216 271 1.21e-13 72.8
MS.gene035555.t1 MTR_5g014040 32.484 157 84 4 131 273 264 412 1.29e-13 73.9
MS.gene035555.t1 MTR_5g014040 35.714 126 75 3 1 124 18 139 5.97e-13 71.6
MS.gene035555.t1 MTR_7g115530 62.500 56 20 1 176 230 216 271 2.17e-13 72.8
MS.gene035555.t1 MTR_4g078810 42.857 84 46 1 176 259 156 237 2.96e-13 72.4
MS.gene035555.t1 MTR_1g080330 60.714 56 21 1 176 230 173 228 3.91e-13 70.5
MS.gene035555.t1 MTR_2g090675 36.752 117 48 4 114 230 123 213 4.15e-13 71.6
MS.gene035555.t1 MTR_1g080330 60.714 56 21 1 176 230 173 228 5.29e-13 71.2
MS.gene035555.t1 MTR_6g088950 30.827 133 82 3 27 155 34 160 2.32e-12 67.0
MS.gene035555.t1 MTR_7g115530 61.111 54 20 1 176 228 216 269 4.17e-12 67.4
MS.gene035555.t1 MTR_7g098250 48.000 75 37 2 178 250 262 336 4.21e-12 68.9
MS.gene035555.t1 MTR_4g081710 49.153 59 29 1 174 231 13 71 5.09e-12 67.8
MS.gene035555.t1 MTR_2g027860 55.000 60 25 2 178 236 49 107 5.49e-12 67.8
MS.gene035555.t1 MTR_6g032990 54.237 59 26 1 174 231 38 96 7.08e-12 67.8
MS.gene035555.t1 MTR_7g115530 60.000 55 21 1 176 229 216 270 1.04e-11 66.2
MS.gene035555.t1 MTR_2g023580 60.000 55 21 1 177 230 54 108 1.51e-11 65.1
MS.gene035555.t1 MTR_5g041350 50.847 59 28 1 174 231 11 69 1.51e-11 66.6
MS.gene035555.t1 MTR_1g093080 44.928 69 36 2 167 234 201 268 1.92e-11 66.2
MS.gene035555.t1 MTR_4g086835 48.276 58 30 0 177 234 273 330 2.45e-11 66.2
MS.gene035555.t1 MTR_2g086450 50.847 59 28 1 174 231 41 99 3.52e-11 65.5
MS.gene035555.t1 MTR_3g450310 49.296 71 29 2 167 230 2 72 5.35e-11 62.8
MS.gene035555.t1 MTR_4g113140 31.250 112 70 3 124 234 187 292 5.73e-11 65.1
MS.gene035555.t1 MTR_0223s0040 54.545 66 29 1 177 241 17 82 6.37e-11 63.9
MS.gene035555.t1 MTR_5g017980 46.552 58 31 0 177 234 191 248 8.42e-11 63.9
MS.gene035555.t1 MTR_5g017980 46.552 58 31 0 177 234 192 249 8.95e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035555.t1 AT2G25180 46.175 366 169 7 1 341 17 379 6.76e-87 281
MS.gene035555.t1 AT1G67710 54.800 250 104 4 1 244 11 257 2.50e-78 256
MS.gene035555.t1 AT4G31920 49.682 314 121 7 1 290 17 317 5.74e-78 256
MS.gene035555.t1 AT5G58080 48.875 311 136 8 1 293 18 323 4.04e-77 256
MS.gene035555.t1 AT3G16857 44.548 321 149 4 1 293 37 356 1.54e-76 255
MS.gene035555.t1 AT3G16857 44.548 321 149 4 1 293 37 356 2.89e-76 255
MS.gene035555.t1 AT2G01760 49.802 253 111 4 1 239 11 261 1.48e-66 221
MS.gene035555.t1 AT3G16857 43.706 286 132 4 36 293 2 286 1.39e-63 219
MS.gene035555.t1 AT2G01760 44.840 281 111 5 1 239 11 289 1.65e-60 206
MS.gene035555.t1 AT1G67710 52.308 195 84 4 56 244 1 192 3.35e-52 185
MS.gene035555.t1 AT5G49240 41.702 235 125 5 1 227 42 272 4.02e-49 172
MS.gene035555.t1 AT2G01760 48.990 198 85 4 56 239 1 196 1.29e-46 166
MS.gene035555.t1 AT3G62670 39.662 237 125 6 2 230 42 268 1.29e-39 150
MS.gene035555.t1 AT1G67710 47.701 174 82 4 77 244 13 183 8.14e-38 145
MS.gene035555.t1 AT1G49190 30.612 294 164 8 3 270 36 315 1.37e-37 144
MS.gene035555.t1 AT5G07210 29.965 287 155 5 1 246 16 297 5.29e-32 130
MS.gene035555.t1 AT5G07210 29.153 295 155 6 1 246 16 305 1.51e-29 123
MS.gene035555.t1 AT2G27070 29.965 287 158 8 1 246 16 300 1.30e-28 120
MS.gene035555.t1 AT2G46790 38.211 123 71 2 1 119 37 158 1.03e-21 98.6
MS.gene035555.t1 AT2G46790 38.211 123 71 2 1 119 37 158 1.14e-21 98.6
MS.gene035555.t1 AT5G24470 36.290 124 74 2 1 120 50 172 1.47e-21 99.0
MS.gene035555.t1 AT1G49190 36.975 119 75 0 3 121 36 154 2.55e-20 95.1
MS.gene035555.t1 AT5G60100 38.525 122 72 1 1 119 64 185 2.18e-19 90.9
MS.gene035555.t1 AT5G60100 38.525 122 72 1 1 119 64 185 2.18e-19 90.9
MS.gene035555.t1 AT5G60100 38.525 122 72 1 1 119 64 185 2.18e-19 90.9
MS.gene035555.t1 AT1G68210 24.444 270 170 4 1 237 13 281 2.53e-19 92.4
MS.gene035555.t1 AT5G60100 38.525 122 72 1 1 119 64 185 3.87e-19 90.9
MS.gene035555.t1 AT5G60100 38.525 122 72 1 1 119 64 185 3.87e-19 90.9
MS.gene035555.t1 AT5G60100 38.525 122 72 1 1 119 64 185 3.87e-19 90.9
MS.gene035555.t1 AT5G60100 38.525 122 72 1 1 119 64 185 6.25e-19 90.5
MS.gene035555.t1 AT5G05090 50.667 75 37 0 160 234 63 137 9.73e-18 84.0
MS.gene035555.t1 AT5G59570 40.385 104 60 1 131 234 96 197 1.26e-17 84.3
MS.gene035555.t1 AT5G59570 40.385 104 60 1 131 234 96 197 1.26e-17 84.3
MS.gene035555.t1 AT3G46640 58.333 60 25 0 177 236 143 202 2.15e-17 84.0
MS.gene035555.t1 AT3G46640 58.333 60 25 0 177 236 143 202 2.15e-17 84.0
MS.gene035555.t1 AT5G02810 34.426 122 77 1 1 119 78 199 3.03e-17 85.9
MS.gene035555.t1 AT3G46640 60.345 58 23 0 177 234 143 200 3.40e-17 83.2
MS.gene035555.t1 AT2G40970 58.621 58 24 0 177 234 104 161 4.65e-17 81.6
MS.gene035555.t1 AT3G10760 54.545 66 30 0 169 234 96 161 5.14e-17 82.8
MS.gene035555.t1 AT5G61380 31.707 123 81 1 1 120 19 141 2.39e-16 82.8
MS.gene035555.t1 AT5G44190 61.818 55 21 0 176 230 145 199 1.73e-15 79.0
MS.gene035555.t1 AT5G44190 61.818 55 21 0 176 230 145 199 1.74e-15 78.6
MS.gene035555.t1 AT2G20570 56.364 55 24 0 176 230 151 205 7.42e-13 71.2
MS.gene035555.t1 AT5G45580 50.000 70 32 2 173 241 4 71 1.00e-12 68.9
MS.gene035555.t1 AT5G45580 50.000 70 32 2 173 241 20 87 1.18e-12 68.9
MS.gene035555.t1 AT2G20570 62.500 48 18 0 183 230 174 221 2.97e-12 69.3
MS.gene035555.t1 AT2G01060 55.000 60 25 2 178 236 15 73 4.47e-12 67.4
MS.gene035555.t1 AT3G04030 52.727 55 25 1 178 231 45 99 2.71e-11 65.9
MS.gene035555.t1 AT4G37180 51.667 60 28 1 176 234 208 267 2.89e-11 65.9
MS.gene035555.t1 AT3G04030 52.727 55 25 1 178 231 45 99 2.91e-11 65.9
MS.gene035555.t1 AT4G37180 53.333 60 27 1 176 234 215 274 3.35e-11 65.5
MS.gene035555.t1 AT5G06800 50.000 66 31 2 178 242 192 256 4.12e-11 64.3
MS.gene035555.t1 AT5G18240 54.545 55 24 1 178 231 45 99 4.38e-11 65.5
MS.gene035555.t1 AT5G18240 54.545 55 24 1 178 231 45 99 4.38e-11 65.5
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 4.41e-11 65.9
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 4.41e-11 65.9
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 4.41e-11 65.9
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 4.41e-11 65.9
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 4.41e-11 65.9
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 4.41e-11 65.9
MS.gene035555.t1 AT5G18240 54.545 55 24 1 178 231 45 99 4.69e-11 65.5
MS.gene035555.t1 AT5G18240 54.545 55 24 1 178 231 45 99 4.69e-11 65.5
MS.gene035555.t1 AT5G18240 54.545 55 24 1 178 231 45 99 4.87e-11 65.5
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 5.82e-11 65.5
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 5.82e-11 65.5
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 5.82e-11 65.5
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 5.82e-11 65.5
MS.gene035555.t1 AT4G18020 36.283 113 69 2 19 130 42 152 5.82e-11 65.5

Find 105 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATTGGTGTCTCGAAAT+TGG 0.196977 8.4:-2005714 None:intergenic
GATTATCTTGCTTCTTCTAA+TGG 0.232806 8.4:+2006271 MS.gene035555:CDS
CCACTTCTGACAGGGTTAAA+AGG 0.242635 8.4:-2006013 None:intergenic
GGACTTAAATTAATGTTATC+AGG 0.249835 8.4:-2005818 None:intergenic
AAGGGTTCAGTAGGTGGATA+TGG 0.252390 8.4:+2005563 MS.gene035555:CDS
AAAAGAAGCCTCGTTTAGTT+TGG 0.279239 8.4:+2005061 MS.gene035555:CDS
CAATGTCAAGATGGCTTACT+TGG 0.323761 8.4:+2006181 MS.gene035555:CDS
TGATTAATGAACTATTAGTT+TGG 0.331944 8.4:-2006324 None:intergenic
CTCACTCAATGGAACAGAAT+TGG 0.343212 8.4:-2006053 None:intergenic
ATCATTAGTGATGGGGTATA+TGG 0.343848 8.4:+2004922 MS.gene035555:CDS
AAAGAAGCCTCGTTTAGTTT+GGG 0.355798 8.4:+2005062 MS.gene035555:CDS
CATTCCTTCCAAGTTCAAAC+TGG 0.363583 8.4:-2005648 None:intergenic
GATAAATATTCTGCTGAAAC+TGG 0.368030 8.4:-2005793 None:intergenic
CACACCATCCAGTTTGAACT+TGG 0.369083 8.4:+2005640 MS.gene035555:CDS
TATCAAATGGTTATCTAAAC+AGG 0.370425 8.4:+2005505 MS.gene035555:CDS
CTTCTCACATGCAACTATAA+TGG 0.375217 8.4:+2002732 MS.gene035555:CDS
GTTTCTGCAGTTAATCTTGT+CGG 0.381222 8.4:+2005108 MS.gene035555:CDS
AACATTGGTGTCTCGAAATT+GGG 0.390536 8.4:-2005713 None:intergenic
ATAAATCACTCTCCTGTTGA+AGG 0.396905 8.4:+2005971 MS.gene035555:CDS
GAGCAATTGCTTAATGTAGA+TGG 0.399600 8.4:+2006418 MS.gene035555:CDS
GATTAATGAACTATTAGTTT+GGG 0.399970 8.4:-2006323 None:intergenic
TCAGCAAGTCGGACTTGAAA+TGG 0.400458 8.4:+2004636 MS.gene035555:CDS
TCCAGCACTTTGAGATAAGT+TGG 0.404451 8.4:-2002702 None:intergenic
AAATGTATCCAGCCATTTGC+AGG 0.408981 8.4:+2005305 MS.gene035555:CDS
ACTGCATTTGAATCATCAAT+TGG 0.413674 8.4:-2005839 None:intergenic
TCAAGTTGAGAAGTTCTATT+CGG 0.435476 8.4:+2006357 MS.gene035555:CDS
ATATAATTTGTACCTGCAAA+TGG 0.440246 8.4:-2005317 None:intergenic
TTCACAGCAGAACCTTCAAC+AGG 0.443727 8.4:-2005983 None:intergenic
TGAATTGTTGAGAGAAAAGA+AGG 0.450895 8.4:+2004552 MS.gene035555:CDS
AGCTTGAAGCCATCCATGTC+TGG 0.455651 8.4:-2004610 None:intergenic
GAGAAGTTCTATTCGGATCA+AGG 0.456955 8.4:+2006364 MS.gene035555:CDS
ACATGGCATTCAAACGTCAA+TGG 0.462461 8.4:+2005763 MS.gene035555:CDS
AGAATTGGAAGCAAAGTTAG+AGG 0.465879 8.4:-2006038 None:intergenic
AGGGTTCAGTAGGTGGATAT+GGG 0.469935 8.4:+2005564 MS.gene035555:CDS
TACTACAACTACGAAGTCAT+TGG 0.471545 8.4:+2004522 MS.gene035555:CDS
TGACAGTGGCTCTGATGTCT+TGG 0.472341 8.4:-2005880 None:intergenic
CTTGACTTGATGAATGTTGA+AGG 0.474751 8.4:+2005270 MS.gene035555:CDS
GATACAGAATTGATATCAAA+TGG 0.480940 8.4:+2005492 MS.gene035555:CDS
TTAAGCTCTTCCATTCGAAT+CGG 0.481435 8.4:-2004841 None:intergenic
CAAGGAATCATGGAATCAAA+TGG 0.482889 8.4:+2006382 MS.gene035555:CDS
TTCAGAATAGTCGTGTGCCT+TGG 0.483059 8.4:+2006446 MS.gene035555:CDS
CATCGTTGCTGTTCCGTTGG+AGG 0.483957 8.4:+2006150 MS.gene035555:CDS
CATTGCATCGTTTCCTCCAA+CGG 0.484353 8.4:-2006163 None:intergenic
TTCCTCCTTCAACAATACCT+TGG 0.487555 8.4:+2006249 MS.gene035555:CDS
AATTTGTACCTGCAAATGGC+TGG 0.488638 8.4:-2005313 None:intergenic
TGGAAGAGCTTAAGAGCATA+TGG 0.489491 8.4:+2004851 MS.gene035555:CDS
GTTGGATCCCTACCAGCAAA+AGG 0.495630 8.4:+2005544 MS.gene035555:CDS
CAGCCGACAAAACATGTCAT+TGG 0.496819 8.4:-2005615 None:intergenic
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.4:+2005280 MS.gene035555:CDS
TTGTTAAAATCCTCACTCAA+TGG 0.497939 8.4:-2006064 None:intergenic
TAGAAAGAAGATTGAAAGCA+AGG 0.500776 8.4:+2004885 MS.gene035555:CDS
TTAGAGGAACCACTTCTGAC+AGG 0.501081 8.4:-2006022 None:intergenic
ATAATCCAAGGTATTGTTGA+AGG 0.508220 8.4:-2006254 None:intergenic
ATCAAGGTATCATTAGTGAT+GGG 0.510346 8.4:+2004914 MS.gene035555:CDS
AATCAAGGTATCATTAGTGA+TGG 0.511362 8.4:+2004913 MS.gene035555:CDS
TGTGTGATTTATAGTGTTGT+CGG 0.513795 8.4:+2004753 MS.gene035555:intron
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8.4:+2004898 MS.gene035555:CDS
CCATCCAGTTTGAACTTGGA+AGG 0.517311 8.4:+2005644 MS.gene035555:CDS
AATGCAGTTACAGATTCCTC+TGG 0.518088 8.4:+2005854 MS.gene035555:CDS
CTTTCACTTTCAGAAAACAT+TGG 0.525109 8.4:-2005728 None:intergenic
TCCAACTTATCTCAAAGTGC+TGG 0.528445 8.4:+2002701 MS.gene035555:CDS
CAATTCTGTTCCATTGAGTG+AGG 0.541688 8.4:+2006054 MS.gene035555:CDS
TGCTAACATCACTAATTACA+AGG 0.543736 8.4:-2004585 None:intergenic
TTCACTTATTGTGTTGTCCA+AGG 0.543954 8.4:-2006463 None:intergenic
CTATTCGGATCAAGGAATCA+TGG 0.544673 8.4:+2006372 MS.gene035555:CDS
CAGCAAAAGGGTTCAGTAGG+TGG 0.548262 8.4:+2005557 MS.gene035555:CDS
TGGTTATCTAAACAGGATAG+AGG 0.553617 8.4:+2005512 MS.gene035555:CDS
CCTTCCAAGTTCAAACTGGA+TGG 0.554980 8.4:-2005644 None:intergenic
AGACATCGTTGCTGTTCCGT+TGG 0.560284 8.4:+2006147 MS.gene035555:CDS
ATGTCATTGGATGCATATGC+TGG 0.565239 8.4:-2005602 None:intergenic
TAATGGAGACACTAGTTCCA+TGG 0.565315 8.4:+2006288 MS.gene035555:CDS
TTTCAAGTCCGACTTGCTGA+AGG 0.572445 8.4:-2004633 None:intergenic
TTGGATCCCTACCAGCAAAA+GGG 0.576563 8.4:+2005545 MS.gene035555:CDS
TTGACTTGATGAATGTTGAA+GGG 0.580486 8.4:+2005271 MS.gene035555:CDS
GAAACGATGCAATGTCAAGA+TGG 0.582127 8.4:+2006172 MS.gene035555:CDS
GGATAGAGGAGATGATGCGT+TGG 0.582696 8.4:+2005526 MS.gene035555:CDS
TTGGGCTAAACTTTGTACCA+TGG 0.585108 8.4:-2006305 None:intergenic
AAGAAAGGTACAAAGTGAGG+AGG 0.585901 8.4:+2004999 MS.gene035555:CDS
GAAAGTTCGAGTTCTTTACA+TGG 0.585974 8.4:+2005746 MS.gene035555:CDS
TTCAAGCTCCTTCAGCAAGT+CGG 0.589889 8.4:+2004625 MS.gene035555:CDS
AACATTTGCATTATTGACAG+TGG 0.591551 8.4:-2005894 None:intergenic
GGGTTCAGTAGGTGGATATG+GGG 0.592030 8.4:+2005565 MS.gene035555:CDS
AGAAGAAGCAAGATAATCCA+AGG 0.597269 8.4:-2006266 None:intergenic
TGATGTTAGCATGCCAGACA+TGG 0.598210 8.4:+2004597 MS.gene035555:CDS
TCTAGTGAAACCGATTCGAA+TGG 0.598674 8.4:+2004831 MS.gene035555:CDS
ATGTTATGAGACTGAAGTGG+AGG 0.606395 8.4:-2005689 None:intergenic
GAATAGAACTTCTCAACTTG+AGG 0.607973 8.4:-2006355 None:intergenic
GCTAACAAGAATAAAACGCA+TGG 0.616521 8.4:+2004970 MS.gene035555:CDS
GGTAAATGATGATATAGAGA+AGG 0.617327 8.4:+2004774 MS.gene035555:CDS
AATGTCAAGATGGCTTACTT+GGG 0.618703 8.4:+2006182 MS.gene035555:CDS
TACCAGCAAAAGGGTTCAGT+AGG 0.620378 8.4:+2005554 MS.gene035555:CDS
TAGAGGAACCACTTCTGACA+GGG 0.620617 8.4:-2006021 None:intergenic
ATCCAAGGTATTGTTGAAGG+AGG 0.626426 8.4:-2006251 None:intergenic
GTCACATAAACTCACTGTCA+AGG 0.629956 8.4:-2005131 None:intergenic
TCAAGGTATCATTAGTGATG+GGG 0.639460 8.4:+2004915 MS.gene035555:CDS
TGTGTGAGCTTACTGATGAA+AGG 0.640199 8.4:-2004664 None:intergenic
TCTGATGTCTTGGAAGCCAG+AGG 0.643398 8.4:-2005870 None:intergenic
CTTAAATTAATGTTATCAGG+CGG 0.647690 8.4:-2005815 None:intergenic
GTTAGCATGCCAGACATGGA+TGG 0.657571 8.4:+2004601 MS.gene035555:CDS
TGAGCTTACTGATGAAAGGT+AGG 0.657693 8.4:-2004660 None:intergenic
GTGATGAAGAGTGTGATACA+TGG 0.658673 8.4:+2004796 MS.gene035555:CDS
TGTTATGAGACTGAAGTGGA+GGG 0.664363 8.4:-2005688 None:intergenic
GGAATGTTATGAGACTGAAG+TGG 0.672395 8.4:-2005692 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.4:+2005281 MS.gene035555:CDS
TCATGATCCCAAACTAAACG+AGG 0.706682 8.4:-2005069 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAAATCTAAAACAAAA+GGG + chr8.4:2004230-2004249 MS.gene035555:intron 10.0%
!!! TTAAAAAAATCTAAAACAAA+AGG + chr8.4:2004229-2004248 MS.gene035555:intron 10.0%
!!! TTTTTATGATAGTAAATAAA+TGG - chr8.4:2003998-2004017 None:intergenic 10.0%
!!! TTTTTTCTTTTTTTTTTTTG+AGG - chr8.4:2003786-2003805 None:intergenic 10.0%
!! AAATAAATGGAAATTATCTT+CGG - chr8.4:2003985-2004004 None:intergenic 15.0%
!! CAATTTACTAAAAAAAACTA+AGG - chr8.4:2002913-2002932 None:intergenic 15.0%
!!! CTTAGTTTTTTTTAGTAAAT+TGG + chr8.4:2002911-2002930 MS.gene035555:intron 15.0%
!! AAATATCCGAATATAAGAAA+TGG - chr8.4:2004729-2004748 None:intergenic 20.0%
!! AATGATTATTACTGCTTTAT+TGG + chr8.4:2004296-2004315 MS.gene035555:intron 20.0%
!! ATAAGAAATGGATCTTAATT+AGG - chr8.4:2004717-2004736 None:intergenic 20.0%
!! GAAGAGACATTTAAATAAAA+AGG - chr8.4:2004462-2004481 None:intergenic 20.0%
!! TAAGATCCATTTCTTATATT+CGG + chr8.4:2004720-2004739 MS.gene035555:intron 20.0%
!! TGATGAATGATTCTTAAATA+TGG + chr8.4:2005183-2005202 MS.gene035555:intron 20.0%
!! TGATTAATGAACTATTAGTT+TGG - chr8.4:2006327-2006346 None:intergenic 20.0%
!!! AAAAATTTGTTTGTTCAGTT+CGG + chr8.4:2003094-2003113 MS.gene035555:intron 20.0%
!!! AGATGTGTTACAATTTTTTA+GGG - chr8.4:2003425-2003444 None:intergenic 20.0%
!!! ATGATTATTACTGCTTTATT+GGG + chr8.4:2004297-2004316 MS.gene035555:intron 20.0%
!!! ATTCTTGTTAGCAATATTTT+CGG - chr8.4:2004962-2004981 None:intergenic 20.0%
!!! GATTAATGAACTATTAGTTT+GGG - chr8.4:2006326-2006345 None:intergenic 20.0%
!!! TTTGAGATTTTTTGACAAAA+GGG - chr8.4:2005351-2005370 None:intergenic 20.0%
!!! TTTTTAGTAAATTGGTGATT+AGG + chr8.4:2002919-2002938 MS.gene035555:intron 20.0%
! AAAAAAATCACTAACATGTC+AGG - chr8.4:2004323-2004342 None:intergenic 25.0%
! AACTACAACCTATTTATCAT+AGG + chr8.4:2003635-2003654 MS.gene035555:intron 25.0%
! AAGTTACTCAATAGTTATAG+TGG - chr8.4:2003162-2003181 None:intergenic 25.0%
! ACACCAATAAAACTTATTTG+TGG + chr8.4:2002985-2003004 MS.gene035555:intron 25.0%
! AGTGTATATTTATGCTTCAT+AGG + chr8.4:2003725-2003744 MS.gene035555:intron 25.0%
! AGTTGAAGAAACTACATATT+AGG - chr8.4:2005220-2005239 None:intergenic 25.0%
! ATTGAATGAACGAAAAAGAA+AGG - chr8.4:2002765-2002784 None:intergenic 25.0%
! CTTAAATTAATGTTATCAGG+CGG - chr8.4:2005818-2005837 None:intergenic 25.0%
! GATACAGAATTGATATCAAA+TGG + chr8.4:2005492-2005511 MS.gene035555:CDS 25.0%
! GGACTTAAATTAATGTTATC+AGG - chr8.4:2005821-2005840 None:intergenic 25.0%
! GTTCTAGTATGATTTAAACA+TGG - chr8.4:2003612-2003631 None:intergenic 25.0%
! TATCAAATGGTTATCTAAAC+AGG + chr8.4:2005505-2005524 MS.gene035555:CDS 25.0%
! TTACATAGCCTATGATAAAT+AGG - chr8.4:2003646-2003665 None:intergenic 25.0%
!! AATTTTCATATGAGAATTGC+TGG + chr8.4:2004412-2004431 MS.gene035555:intron 25.0%
!! ATATAATTTGTACCTGCAAA+TGG - chr8.4:2005320-2005339 None:intergenic 25.0%
!! ATCAAGTCAAGTATTTTCTT+AGG - chr8.4:2005261-2005280 None:intergenic 25.0%
!! GGTCTTTTATATAATAATGC+TGG + chr8.4:2004433-2004452 MS.gene035555:intron 25.0%
!! TTTTCTGCATCATTAAAAAC+CGG + chr8.4:2003468-2003487 MS.gene035555:intron 25.0%
!! TTTTTCCTTCTTTTTCTGAA+TGG + chr8.4:2004342-2004361 MS.gene035555:intron 25.0%
!!! CAAAACAGATTTTAAGAGAA+AGG - chr8.4:2004690-2004709 None:intergenic 25.0%
!!! GAAACCTTTTTTTTTCTTTG+AGG + chr8.4:2002872-2002891 MS.gene035555:intron 25.0%
!!! GAGATGTGTTACAATTTTTT+AGG - chr8.4:2003426-2003445 None:intergenic 25.0%
!!! GTTTGAGATTTTTTGACAAA+AGG - chr8.4:2005352-2005371 None:intergenic 25.0%
!!! TTGAATAACTTTTCGATTTG+AGG + chr8.4:2004095-2004114 MS.gene035555:intron 25.0%
AACATTTGCATTATTGACAG+TGG - chr8.4:2005897-2005916 None:intergenic 30.0%
AACGCCTCAAAGAAAAAAAA+AGG - chr8.4:2002879-2002898 None:intergenic 30.0%
ACTCAATAGTTATAGTGGTA+CGG - chr8.4:2003157-2003176 None:intergenic 30.0%
ACTGCATTTGAATCATCAAT+TGG - chr8.4:2005842-2005861 None:intergenic 30.0%
ACTTCTGTCTCGTAAAAAAT+CGG - chr8.4:2003518-2003537 None:intergenic 30.0%
ATAGGCTATGTAAATGTATC+CGG + chr8.4:2003653-2003672 MS.gene035555:intron 30.0%
ATCCTAATAGTGTTTCTCTA+AGG + chr8.4:2003243-2003262 MS.gene035555:intron 30.0%
ATGTAGAGAGTAGAAAAACA+AGG - chr8.4:2003355-2003374 None:intergenic 30.0%
CTTTCACTTTCAGAAAACAT+TGG - chr8.4:2005731-2005750 None:intergenic 30.0%
CTTTGGCAAAAGAATGAATT+AGG - chr8.4:2003191-2003210 None:intergenic 30.0%
GATAAATATTCTGCTGAAAC+TGG - chr8.4:2005796-2005815 None:intergenic 30.0%
GATTATCTTGCTTCTTCTAA+TGG + chr8.4:2006271-2006290 MS.gene035555:CDS 30.0%
GGTAAATGATGATATAGAGA+AGG + chr8.4:2004774-2004793 MS.gene035555:CDS 30.0%
TAAACCTTGTATCAGATGAA+AGG - chr8.4:2003384-2003403 None:intergenic 30.0%
TAGAAACCACAACAAAACTA+GGG - chr8.4:2003329-2003348 None:intergenic 30.0%
TAGAAAGAAGATTGAAAGCA+AGG + chr8.4:2004885-2004904 MS.gene035555:CDS 30.0%
TCAAAACCACACCTAAAATT+GGG + chr8.4:2003539-2003558 MS.gene035555:intron 30.0%
TGAATTGTTGAGAGAAAAGA+AGG + chr8.4:2004552-2004571 MS.gene035555:CDS 30.0%
TGCAATCGAAAATGAAAATG+AGG - chr8.4:2005470-2005489 None:intergenic 30.0%
TGCTAACATCACTAATTACA+AGG - chr8.4:2004588-2004607 None:intergenic 30.0%
TGTAGAGAGTAGAAAAACAA+GGG - chr8.4:2003354-2003373 None:intergenic 30.0%
TGTGTGATTTATAGTGTTGT+CGG + chr8.4:2004753-2004772 MS.gene035555:intron 30.0%
TTAGAAACCACAACAAAACT+AGG - chr8.4:2003330-2003349 None:intergenic 30.0%
TTCTTCAACTATTATTGCAG+AGG + chr8.4:2005230-2005249 MS.gene035555:intron 30.0%
TTGTTAAAATCCTCACTCAA+TGG - chr8.4:2006067-2006086 None:intergenic 30.0%
TTTCAACCGTATTTGCAAAA+AGG - chr8.4:2005400-2005419 None:intergenic 30.0%
! AAAAAGAAAGGTACAAAGTG+AGG + chr8.4:2004996-2005015 MS.gene035555:CDS 30.0%
! AATCAAGGTATCATTAGTGA+TGG + chr8.4:2004913-2004932 MS.gene035555:CDS 30.0%
! ATCAAGGTATCATTAGTGAT+GGG + chr8.4:2004914-2004933 MS.gene035555:CDS 30.0%
! TAAATCACTAGCTTGATTCT+TGG + chr8.4:2004484-2004503 MS.gene035555:intron 30.0%
! TTGACTTGATGAATGTTGAA+GGG + chr8.4:2005271-2005290 MS.gene035555:CDS 30.0%
! TTTTGATGTTGTGAGTAACA+TGG - chr8.4:2006223-2006242 None:intergenic 30.0%
! TTTTGCCATTCAGAAAAAGA+AGG - chr8.4:2004350-2004369 None:intergenic 30.0%
!! ATAATCCAAGGTATTGTTGA+AGG - chr8.4:2006257-2006276 None:intergenic 30.0%
!! GCTCCACAAATAAGTTTTAT+TGG - chr8.4:2002991-2003010 None:intergenic 30.0%
!! TCAAGTTGAGAAGTTCTATT+CGG + chr8.4:2006357-2006376 MS.gene035555:CDS 30.0%
!! TTGGAAGTAGCTTTGATATA+AGG + chr8.4:2002850-2002869 MS.gene035555:intron 30.0%
AAAAGAAGCCTCGTTTAGTT+TGG + chr8.4:2005061-2005080 MS.gene035555:CDS 35.0%
AACGCATGGACAAAAAAGAA+AGG + chr8.4:2004984-2005003 MS.gene035555:CDS 35.0%
AATGTCAAGATGGCTTACTT+GGG + chr8.4:2006182-2006201 MS.gene035555:CDS 35.0%
ACTATTAGGATTGAGTCTCT+TGG - chr8.4:2003234-2003253 None:intergenic 35.0%
AGAAGAAGCAAGATAATCCA+AGG - chr8.4:2006269-2006288 None:intergenic 35.0%
AGAATTGGAAGCAAAGTTAG+AGG - chr8.4:2006041-2006060 None:intergenic 35.0%
ATAAATCACTCTCCTGTTGA+AGG + chr8.4:2005971-2005990 MS.gene035555:CDS 35.0%
CAAGGAATCATGGAATCAAA+TGG + chr8.4:2006382-2006401 MS.gene035555:CDS 35.0%
CACCTTAGAGAAACACTATT+AGG - chr8.4:2003248-2003267 None:intergenic 35.0%
CACTAATACATATCAGACTC+CGG - chr8.4:2003675-2003694 None:intergenic 35.0%
CTCAAAACCACACCTAAAAT+TGG + chr8.4:2003538-2003557 MS.gene035555:intron 35.0%
CTTCTCACATGCAACTATAA+TGG + chr8.4:2002732-2002751 MS.gene035555:CDS 35.0%
GAAAGCAAGGATCAAAATCA+AGG + chr8.4:2004898-2004917 MS.gene035555:CDS 35.0%
GAATAGAACTTCTCAACTTG+AGG - chr8.4:2006358-2006377 None:intergenic 35.0%
GAGCAATTGCTTAATGTAGA+TGG + chr8.4:2006418-2006437 MS.gene035555:CDS 35.0%
GCAACGGTGTAAACATATAT+TGG + chr8.4:2002831-2002850 MS.gene035555:intron 35.0%
GCTAACAAGAATAAAACGCA+TGG + chr8.4:2004970-2004989 MS.gene035555:CDS 35.0%
GTCGAAGAAAAATGAGAACT+GGG - chr8.4:2006127-2006146 None:intergenic 35.0%
GTTTCTGCAGTTAATCTTGT+CGG + chr8.4:2005108-2005127 MS.gene035555:CDS 35.0%
TACTACAACTACGAAGTCAT+TGG + chr8.4:2004522-2004541 MS.gene035555:CDS 35.0%
TCTCTTTGCAACAAACACTA+GGG - chr8.4:2003294-2003313 None:intergenic 35.0%
TGGTTATCTAAACAGGATAG+AGG + chr8.4:2005512-2005531 MS.gene035555:CDS 35.0%
TGTCGAAGAAAAATGAGAAC+TGG - chr8.4:2006128-2006147 None:intergenic 35.0%
TTAAGCTCTTCCATTCGAAT+CGG - chr8.4:2004844-2004863 None:intergenic 35.0%
TTCTCTTTGCAACAAACACT+AGG - chr8.4:2003295-2003314 None:intergenic 35.0%
! AAACATTGGTGTCTCGAAAT+TGG - chr8.4:2005717-2005736 None:intergenic 35.0%
! AAAGAAGCCTCGTTTAGTTT+GGG + chr8.4:2005062-2005081 MS.gene035555:CDS 35.0%
! AACATTGGTGTCTCGAAATT+GGG - chr8.4:2005716-2005735 None:intergenic 35.0%
! ATCATTAGTGATGGGGTATA+TGG + chr8.4:2004922-2004941 MS.gene035555:CDS 35.0%
! CATCCAATGACATGTTTTGT+CGG + chr8.4:2005612-2005631 MS.gene035555:CDS 35.0%
! CTTGACTTGATGAATGTTGA+AGG + chr8.4:2005270-2005289 MS.gene035555:CDS 35.0%
! GAAAGTTCGAGTTCTTTACA+TGG + chr8.4:2005746-2005765 MS.gene035555:CDS 35.0%
! GTTAGCAATATTTTCGGATG+AGG - chr8.4:2004956-2004975 None:intergenic 35.0%
! TCAAGGTATCATTAGTGATG+GGG + chr8.4:2004915-2004934 MS.gene035555:CDS 35.0%
! TCACCTCCTTTTTGCAAATA+CGG + chr8.4:2005391-2005410 MS.gene035555:intron 35.0%
! TTTGAGGTAGCTTAGACATT+CGG - chr8.4:2003770-2003789 None:intergenic 35.0%
!!! GGTGATTAGGTTAGTTTTCA+CGG + chr8.4:2002932-2002951 MS.gene035555:intron 35.0%
!!! GTTAGTTTTCACGGTTTGAA+CGG + chr8.4:2002941-2002960 MS.gene035555:intron 35.0%
!!! TACAAACCCTAGTTTTGTTG+TGG + chr8.4:2003320-2003339 MS.gene035555:intron 35.0%
AAATGTATCCAGCCATTTGC+AGG + chr8.4:2005305-2005324 MS.gene035555:CDS 40.0%
AATGCAGTTACAGATTCCTC+TGG + chr8.4:2005854-2005873 MS.gene035555:CDS 40.0%
AATTTGTACCTGCAAATGGC+TGG - chr8.4:2005316-2005335 None:intergenic 40.0%
ACATGGCATTCAAACGTCAA+TGG + chr8.4:2005763-2005782 MS.gene035555:CDS 40.0%
ACTATTCTCCTCTCATGCAA+CGG + chr8.4:2002815-2002834 MS.gene035555:intron 40.0%
ATCAAGCAACCAAAACAACG+TGG + chr8.4:2003017-2003036 MS.gene035555:intron 40.0%
ATGTCATTGGATGCATATGC+TGG - chr8.4:2005605-2005624 None:intergenic 40.0%
ATGTTATGAGACTGAAGTGG+AGG - chr8.4:2005692-2005711 None:intergenic 40.0%
CAACCGTATTTGCAAAAAGG+AGG - chr8.4:2005397-2005416 None:intergenic 40.0%
CAACTGATATGTGTGTGAGT+AGG - chr8.4:2003825-2003844 None:intergenic 40.0%
CAATGTCAAGATGGCTTACT+TGG + chr8.4:2006181-2006200 MS.gene035555:CDS 40.0%
CAATTCTGTTCCATTGAGTG+AGG + chr8.4:2006054-2006073 MS.gene035555:CDS 40.0%
CATTCCTTCCAAGTTCAAAC+TGG - chr8.4:2005651-2005670 None:intergenic 40.0%
CTATTCGGATCAAGGAATCA+TGG + chr8.4:2006372-2006391 MS.gene035555:CDS 40.0%
CTCACCTTTCATCTGATACA+AGG + chr8.4:2003377-2003396 MS.gene035555:intron 40.0%
CTCACTCAATGGAACAGAAT+TGG - chr8.4:2006056-2006075 None:intergenic 40.0%
GAAACGATGCAATGTCAAGA+TGG + chr8.4:2006172-2006191 MS.gene035555:CDS 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.4:2005281-2005300 MS.gene035555:CDS 40.0%
GAGAAGTTCTATTCGGATCA+AGG + chr8.4:2006364-2006383 MS.gene035555:CDS 40.0%
GGAATGTTATGAGACTGAAG+TGG - chr8.4:2005695-2005714 None:intergenic 40.0%
GTCACATAAACTCACTGTCA+AGG - chr8.4:2005134-2005153 None:intergenic 40.0%
TAATGGAGACACTAGTTCCA+TGG + chr8.4:2006288-2006307 MS.gene035555:CDS 40.0%
TCAAGCAACCAAAACAACGT+GGG + chr8.4:2003018-2003037 MS.gene035555:intron 40.0%
TCATGATCCCAAACTAAACG+AGG - chr8.4:2005072-2005091 None:intergenic 40.0%
TCCAACTTATCTCAAAGTGC+TGG + chr8.4:2002701-2002720 MS.gene035555:CDS 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.4:2005280-2005299 MS.gene035555:CDS 40.0%
TGAGCTTACTGATGAAAGGT+AGG - chr8.4:2004663-2004682 None:intergenic 40.0%
TGTGTGAGCTTACTGATGAA+AGG - chr8.4:2004667-2004686 None:intergenic 40.0%
TGTTATGAGACTGAAGTGGA+GGG - chr8.4:2005691-2005710 None:intergenic 40.0%
TTCCTCCTTCAACAATACCT+TGG + chr8.4:2006249-2006268 MS.gene035555:CDS 40.0%
TTGAGGTAGCTTAGACATTC+GGG - chr8.4:2003769-2003788 None:intergenic 40.0%
TTGCAACAAACACTAGGGTT+GGG - chr8.4:2003289-2003308 None:intergenic 40.0%
TTGGGCTAAACTTTGTACCA+TGG - chr8.4:2006308-2006327 None:intergenic 40.0%
TTTGCAACAAACACTAGGGT+TGG - chr8.4:2003290-2003309 None:intergenic 40.0%
! AAGAAAGGTACAAAGTGAGG+AGG + chr8.4:2004999-2005018 MS.gene035555:CDS 40.0%
! ACTAAACGAGGCTTCTTTTG+AGG - chr8.4:2005060-2005079 None:intergenic 40.0%
! GTGATGAAGAGTGTGATACA+TGG + chr8.4:2004796-2004815 MS.gene035555:CDS 40.0%
! TCTGAAACTGCCAAAATCGA+CGG - chr8.4:2003585-2003604 None:intergenic 40.0%
! TCTTTTGCCAAGACTGTTGT+GGG + chr8.4:2003878-2003897 MS.gene035555:intron 40.0%
! TGGAAGAGCTTAAGAGCATA+TGG + chr8.4:2004851-2004870 MS.gene035555:CDS 40.0%
! TGTTGTGGGTGTGTTTGTTT+TGG + chr8.4:2003892-2003911 MS.gene035555:intron 40.0%
! TTCTTTTGCCAAGACTGTTG+TGG + chr8.4:2003877-2003896 MS.gene035555:intron 40.0%
!! ATCCAAGGTATTGTTGAAGG+AGG - chr8.4:2006254-2006273 None:intergenic 40.0%
!! TCCAGCACTTTGAGATAAGT+TGG - chr8.4:2002705-2002724 None:intergenic 40.0%
!! TCTAGTGAAACCGATTCGAA+TGG + chr8.4:2004831-2004850 MS.gene035555:CDS 40.0%
AAACACACCCACAACAGTCT+TGG - chr8.4:2003888-2003907 None:intergenic 45.0%
AAGGGTTCAGTAGGTGGATA+TGG + chr8.4:2005563-2005582 MS.gene035555:CDS 45.0%
AGGATTGAGTCTCTTGGTCA+CGG - chr8.4:2003228-2003247 None:intergenic 45.0%
AGGGTTCAGTAGGTGGATAT+GGG + chr8.4:2005564-2005583 MS.gene035555:CDS 45.0%
CACACCATCCAGTTTGAACT+TGG + chr8.4:2005640-2005659 MS.gene035555:CDS 45.0%
CAGCCGACAAAACATGTCAT+TGG - chr8.4:2005618-2005637 None:intergenic 45.0%
CATTGCATCGTTTCCTCCAA+CGG - chr8.4:2006166-2006185 None:intergenic 45.0%
CCAATACTGACCTACTTTGG+AGG + chr8.4:2003118-2003137 MS.gene035555:intron 45.0%
CCACTTCTGACAGGGTTAAA+AGG - chr8.4:2006016-2006035 None:intergenic 45.0%
CCATCCAGTTTGAACTTGGA+AGG + chr8.4:2005644-2005663 MS.gene035555:CDS 45.0%
CCTCCAAAGTAGGTCAGTAT+TGG - chr8.4:2003121-2003140 None:intergenic 45.0%
CCTTCCAAGTTCAAACTGGA+TGG - chr8.4:2005647-2005666 None:intergenic 45.0%
CGGTTTGAACGGTTGTGAAT+CGG + chr8.4:2002952-2002971 MS.gene035555:intron 45.0%
CTTGGTCACGGAAAGAGATT+CGG - chr8.4:2003216-2003235 None:intergenic 45.0%
GAAGTGAGAAAATCGGAGTC+CGG - chr8.4:2003490-2003509 None:intergenic 45.0%
GGTCCAATACTGACCTACTT+TGG + chr8.4:2003115-2003134 MS.gene035555:intron 45.0%
TACCAGCAAAAGGGTTCAGT+AGG + chr8.4:2005554-2005573 MS.gene035555:CDS 45.0%
TAGAGGAACCACTTCTGACA+GGG - chr8.4:2006024-2006043 None:intergenic 45.0%
TCAGCAAGTCGGACTTGAAA+TGG + chr8.4:2004636-2004655 MS.gene035555:CDS 45.0%
TGATGTTAGCATGCCAGACA+TGG + chr8.4:2004597-2004616 MS.gene035555:CDS 45.0%
TGTTTACACCGTTGCATGAG+AGG - chr8.4:2002826-2002845 None:intergenic 45.0%
TTAGAGGAACCACTTCTGAC+AGG - chr8.4:2006025-2006044 None:intergenic 45.0%
TTCAAGCTCCTTCAGCAAGT+CGG + chr8.4:2004625-2004644 MS.gene035555:CDS 45.0%
TTCACAGCAGAACCTTCAAC+AGG - chr8.4:2005986-2006005 None:intergenic 45.0%
TTCAGAATAGTCGTGTGCCT+TGG + chr8.4:2006446-2006465 MS.gene035555:CDS 45.0%
TTGGATCCCTACCAGCAAAA+GGG + chr8.4:2005545-2005564 MS.gene035555:CDS 45.0%
TTGGTCACGGAAAGAGATTC+GGG - chr8.4:2003215-2003234 None:intergenic 45.0%
! ACTGAACCCTTTTGCTGGTA+GGG - chr8.4:2005554-2005573 None:intergenic 45.0%
! AGCAATCGAGGCCCAATTTT+AGG - chr8.4:2003553-2003572 None:intergenic 45.0%
! CCTTTTAACCCTGTCAGAAG+TGG + chr8.4:2006013-2006032 MS.gene035555:CDS 45.0%
! GACTCCGATTTTCTCACTTC+GGG + chr8.4:2003490-2003509 MS.gene035555:intron 45.0%
! GGACTCCGATTTTCTCACTT+CGG + chr8.4:2003489-2003508 MS.gene035555:intron 45.0%
! GTGTGAGTAGGTAGCTTTAC+TGG - chr8.4:2003813-2003832 None:intergenic 45.0%
! TACTGAACCCTTTTGCTGGT+AGG - chr8.4:2005555-2005574 None:intergenic 45.0%
!! TTTCAAGTCCGACTTGCTGA+AGG - chr8.4:2004636-2004655 None:intergenic 45.0%
!!! AAAATATTTTGTTAAAAAAA+TGG - chr8.4:2004133-2004152 None:intergenic 5.0%
!!! AAATTATATAAGTATTTTTT+TGG + chr8.4:2004180-2004199 MS.gene035555:intron 5.0%
AGCAACCAAAACAACGTGGG+TGG + chr8.4:2003021-2003040 MS.gene035555:intron 50.0%
AGCTTGAAGCCATCCATGTC+TGG - chr8.4:2004613-2004632 None:intergenic 50.0%
CAGCAAAAGGGTTCAGTAGG+TGG + chr8.4:2005557-2005576 MS.gene035555:CDS 50.0%
CGGAAAGAGATTCGGGACTT+TGG - chr8.4:2003208-2003227 None:intergenic 50.0%
GGATAGAGGAGATGATGCGT+TGG + chr8.4:2005526-2005545 MS.gene035555:CDS 50.0%
GGGTTCAGTAGGTGGATATG+GGG + chr8.4:2005565-2005584 MS.gene035555:CDS 50.0%
GTTAGCATGCCAGACATGGA+TGG + chr8.4:2004601-2004620 MS.gene035555:CDS 50.0%
GTTGGATCCCTACCAGCAAA+AGG + chr8.4:2005544-2005563 MS.gene035555:CDS 50.0%
TCTGATGTCTTGGAAGCCAG+AGG - chr8.4:2005873-2005892 None:intergenic 50.0%
! AGACATCGTTGCTGTTCCGT+TGG + chr8.4:2006147-2006166 MS.gene035555:CDS 50.0%
! CACCTACTGAACCCTTTTGC+TGG - chr8.4:2005559-2005578 None:intergenic 50.0%
!! TCGAGGCCCAATTTTAGGTG+TGG - chr8.4:2003548-2003567 None:intergenic 50.0%
!! TGACAGTGGCTCTGATGTCT+TGG - chr8.4:2005883-2005902 None:intergenic 50.0%
!!! TCCAAATCGGCCGTCGATTT+TGG + chr8.4:2003572-2003591 MS.gene035555:intron 50.0%
GCCAAAATCGACGGCCGATT+TGG - chr8.4:2003576-2003595 None:intergenic 55.0%
GCTGCTCTCTCCTCCAAAGT+AGG - chr8.4:2003131-2003150 None:intergenic 55.0%
GGCGCCCGAAGTGAGAAAAT+CGG - chr8.4:2003497-2003516 None:intergenic 55.0%
GGGCCTCGATTGCTCCAAAT+CGG + chr8.4:2003559-2003578 MS.gene035555:intron 55.0%
! AGCTGCCACCCACGTTGTTT+TGG - chr8.4:2003029-2003048 None:intergenic 55.0%
! CATCGTTGCTGTTCCGTTGG+AGG + chr8.4:2006150-2006169 MS.gene035555:CDS 55.0%
!! CGGCCGATTTGGAGCAATCG+AGG - chr8.4:2003565-2003584 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 2002675 2006480 2002675 ID=MS.gene035555
chr8.4 mRNA 2002675 2006480 2002675 ID=MS.gene035555.t1;Parent=MS.gene035555
chr8.4 exon 2002675 2002753 2002675 ID=MS.gene035555.t1.exon1;Parent=MS.gene035555.t1
chr8.4 CDS 2002675 2002753 2002675 ID=cds.MS.gene035555.t1;Parent=MS.gene035555.t1
chr8.4 exon 2004521 2004673 2004521 ID=MS.gene035555.t1.exon2;Parent=MS.gene035555.t1
chr8.4 CDS 2004521 2004673 2004521 ID=cds.MS.gene035555.t1;Parent=MS.gene035555.t1
chr8.4 exon 2004767 2005138 2004767 ID=MS.gene035555.t1.exon3;Parent=MS.gene035555.t1
chr8.4 CDS 2004767 2005138 2004767 ID=cds.MS.gene035555.t1;Parent=MS.gene035555.t1
chr8.4 exon 2005250 2005326 2005250 ID=MS.gene035555.t1.exon4;Parent=MS.gene035555.t1
chr8.4 CDS 2005250 2005326 2005250 ID=cds.MS.gene035555.t1;Parent=MS.gene035555.t1
chr8.4 exon 2005491 2006480 2005491 ID=MS.gene035555.t1.exon5;Parent=MS.gene035555.t1
chr8.4 CDS 2005491 2006480 2005491 ID=cds.MS.gene035555.t1;Parent=MS.gene035555.t1
Gene Sequence

>MS.gene035555

ATGCGTGTTCTGGCAGTTGATGACGATCCAACTTATCTCAAAGTGCTGGAGAAGCAACTTCTCACATGCAACTATAATGGTTCGTTGCCTTTCTTTTTCGTTCATTCAATATGATTACGCATTGATTATTCTTTATGTTTACTATTCTCCTCTCATGCAACGGTGTAAACATATATTGGAAGTAGCTTTGATATAAGGAAACCTTTTTTTTTCTTTGAGGCGTTGATATAGTAAACCTTAGTTTTTTTTAGTAAATTGGTGATTAGGTTAGTTTTCACGGTTTGAACGGTTGTGAATCGGTTAAAAAATCACACCAATAAAACTTATTTGTGGAGCAAATGAATCAAGCAACCAAAACAACGTGGGTGGCAGCTCTAAACACAATCAAAAGATAGAACAACAAAAGAGAAAGAACACACAAAAATTTGTTTGTTCAGTTCGGTCCAATACTGACCTACTTTGGAGGAGAGAGCAGCTCTCCGTACCACTATAACTATTGAGTAACTTTACAAACCTAATTCATTCTTTTGCCAAAGTCCCGAATCTCTTTCCGTGACCAAGAGACTCAATCCTAATAGTGTTTCTCTAAGGTGAATCAATGATGACTCAATCCCAACCCTAGTGTTTGTTGCAAAGAGAAAACTCTACAAACCCTAGTTTTGTTGTGGTTTCTAAACCCTTGTTTTTCTACTCTCTACATCTCTCACCTTTCATCTGATACAAGGTTTATATTTATAGTAAAAGCATCCCTAAAAAATTGTAACACATCTCATGCTGAAAATACGTTTATGTTTTTTCTGCATCATTAAAAACCGGACTCCGATTTTCTCACTTCGGGCGCCGATTTTTTACGAGACAGAAGTCTCAAAACCACACCTAAAATTGGGCCTCGATTGCTCCAAATCGGCCGTCGATTTTGGCAGTTTCAGAAACACCATGTTTAAATCATACTAGAACATGAACTACAACCTATTTATCATAGGCTATGTAAATGTATCCGGAGTCTGATATGTATTAGTGTCTGATACAATTTGACGCATGTCATGTGTTAGTGTATATTTATGCTTCATAGGTTATAAGTCTTCTAGTGTCCCGAATGTCTAAGCTACCTCAAAAAAAAAAAAGAAAAAAACTACCAGTAAAGCTACCTACTCACACACATATCAGTTGACAATTTTCTTGATTTTAAATACATAAATTCATTCTTTTGCCAAGACTGTTGTGGGTGTGTTTGTTTTGGCTTAAAATAAAATTATTTTGTTTTTATAAAATATAAATATTATTTTTGAGATCTATAAAAAAAAAAGCCGAAGATAATTTCCATTTATTTACTATCATAAAAATAATTTTTTTAAGATTTAAAACACTTACACTTGAGTTTCTTGTTTTTTAAAAGAAAAATAGATTTTGAAAAAGCTATTTGAATAACTTTTCGATTTGAGGCTAAAAATTACTCCATTTTTTTAACAAAATATTTTTAAAAAAAATCTGAAACAAACACTATAAAATTATATAAGTATTTTTTTGGTTTAAAAAATTGATTTTTTGTTTCTTTTAAAAAAATCTAAAACAAAAGGGTCTGTGTTTTCTCTACTTCTAAAGTTGAAATTTTAAACTTTTTAATGATTATTACTGCTTTATTGGGCCTGACATGTTAGTGATTTTTTTTTTTCCTTCTTTTTCTGAATGGCAAAAGAAAATTTTATAAAATAAGATGATACTAATCTTATTTAATAAAATTTTCATATGAGAATTGCTGGTCTTTTATATAATAATGCTGGAAGCCTTTTTATTTAAATGTCTCTTCACTAAATCACTAGCTTGATTCTTGGTTGCATTGTTGCAGTTACTACAACTACGAAGTCATTGGAAGCGCTTGAATTGTTGAGAGAAAAGAAGGATATGTTTGACCTTGTAATTAGTGATGTTAGCATGCCAGACATGGATGGCTTCAAGCTCCTTCAGCAAGTCGGACTTGAAATGGACCTACCTTTCATCAGTAAGCTCACACACCTTTCTCTTAAAATCTGTTTTGTTTACCTAATTAAGATCCATTTCTTATATTCGGATATTTTGTGTGTGTGATTTATAGTGTTGTCGGTAAATGATGATATAGAGAAGGTGATGAAGAGTGTGATACATGGTGCTTGCAACTATCTAGTGAAACCGATTCGAATGGAAGAGCTTAAGAGCATATGGCAACATGTAGTTAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCATTAGTGATGGGGTATATGGTCAAGACACCTCATCCGAAAATATTGCTAACAAGAATAAAACGCATGGACAAAAAAGAAAGGTACAAAGTGAGGAGGAAGAAGCAGAACAAGATAATGATGAGAAACGTTCGCCTCAAAAGAAGCCTCGTTTAGTTTGGGATCATGAGTTGCATCGAAAATTTGTTTCTGCAGTTAATCTTGTCGGCCTTGACAGTGAGTTTATGTGACAATGAAATTATTTTTTTACTTTGCTAATTTGATGAATGATTCTTAAATATGGATATTATTGTTCCTAATATGTAGTTTCTTCAACTATTATTGCAGAGGCTTCGCCTAAGAAAATACTTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTATCCAGCCATTTGCAGGTACAAATTATATATAACTTTCCCTTTTGTCAAAAAATCTCAAACTAGTTTATCATTAAAATATTCACCTCCTTTTTGCAAATACGGTTGAAACTTCAACAAGCAAGTTTATGAAACGTAAGTATAAAAACTGTGTGTAACCTCATTTTCATTTTCGATTGCAGAGATACAGAATTGATATCAAATGGTTATCTAAACAGGATAGAGGAGATGATGCGTTGGATCCCTACCAGCAAAAGGGTTCAGTAGGTGGATATGGGGATTTTTGCACACTGCCAGCATATGCATCCAATGACATGTTTTGTCGGCTGAACACACCATCCAGTTTGAACTTGGAAGGAATGAGTTCTTCTGCACTAGTCCCTCCACTTCAGTCTCATAACATTCCCAATTTCGAGACACCAATGTTTTCTGAAAGTGAAAGTTCGAGTTCTTTACATGGCATTCAAACGTCAATGGAAATTAACCAGTTTCAGCAGAATATTTATCCGCCTGATAACATTAATTTAAGTCCAATTGATGATTCAAATGCAGTTACAGATTCCTCTGGCTTCCAAGACATCAGAGCCACTGTCAATAATGCAAATGTTTCTCTTTCTTGCATCTCAAGCAATCACATACAAACACATAAATCAGAAGCATTTATAAATCACTCTCCTGTTGAAGGTTCTGCTGTGAAAACACAACCTTTTAACCCTGTCAGAAGTGGTTCCTCTAACTTTGCTTCCAATTCTGTTCCATTGAGTGAGGATTTTAACAATGATCAAATGTTGCACAATAACTACAAGTTTACTTCTCCCAGTTCTCATTTTTCTTCGACAGACATCGTTGCTGTTCCGTTGGAGGAAACGATGCAATGTCAAGATGGCTTACTTGGGAATGTTATAAATGCTCCATGTTACTCACAACATCAAAATGCGAGTTCCTCCTTCAACAATACCTTGGATTATCTTGCTTCTTCTAATGGAGACACTAGTTCCATGGTACAAAGTTTAGCCCAAACTAATAGTTCATTAATCACTCAGATGCCTCAAGTTGAGAAGTTCTATTCGGATCAAGGAATCATGGAATCAAATGGTGATTGCTTTGTTGAGCAATTGCTTAATGTAGATGGATTCATTCAGAATAGTCGTGTGCCTTGGACAACACAATAA
Protein sequence

>MS.gene035555.t1

MRVLAVDDDPTYLKVLEKQLLTCNYNVTTTTKSLEALELLREKKDMFDLVISDVSMPDMDGFKLLQQVGLEMDLPFIMLSVNDDIEKVMKSVIHGACNYLVKPIRMEELKSIWQHVVRKKIESKDQNQGIISDGVYGQDTSSENIANKNKTHGQKRKVQSEEEEAEQDNDEKRSPQKKPRLVWDHELHRKFVSAVNLVGLDKASPKKILDLMNVEGLTRENVSSHLQRYRIDIKWLSKQDRGDDALDPYQQKGSVGGYGDFCTLPAYASNDMFCRLNTPSSLNLEGMSSSALVPPLQSHNIPNFETPMFSESESSSSLHGIQTSMEINQFQQNIYPPDNINLSPIDDSNAVTDSSGFQDIRATVNNANVSLSCISSNHIQTHKSEAFINHSPVEGSAVKTQPFNPVRSGSSNFASNSVPLSEDFNNDQMLHNNYKFTSPSSHFSSTDIVAVPLEETMQCQDGLLGNVINAPCYSQHQNASSSFNNTLDYLASSNGDTSSMVQSLAQTNSSLITQMPQVEKFYSDQGIMESNGDCFVEQLLNVDGFIQNSRVPWTTQ