AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035560.t1 MTR_4g131580 94.807 597 31 0 1 597 1 597 0.0 1125
MS.gene035560.t1 MTR_4g131570 77.234 593 126 4 1 588 1 589 0.0 889
MS.gene035560.t1 MTR_4g131600 71.864 590 158 3 1 590 4 585 0.0 818
MS.gene035560.t1 MTR_2g450070 44.150 641 268 19 3 566 4 631 6.25e-128 390
MS.gene035560.t1 MTR_2g450070 44.150 641 268 19 3 566 4 631 7.08e-128 391
MS.gene035560.t1 MTR_3g106220 44.888 401 182 9 17 394 8 392 9.57e-93 298
MS.gene035560.t1 MTR_1g013170 47.135 384 176 10 17 382 5 379 5.14e-92 292
MS.gene035560.t1 MTR_1g032570 48.464 293 138 6 17 297 11 302 1.78e-83 274
MS.gene035560.t1 MTR_4g121020 42.559 383 184 7 17 375 31 401 8.26e-82 271
MS.gene035560.t1 MTR_4g121020 42.559 383 184 7 17 375 31 401 8.44e-82 271
MS.gene035560.t1 MTR_1g013180 49.186 307 146 3 17 314 156 461 1.32e-79 265
MS.gene035560.t1 MTR_2g034960 46.687 332 158 7 9 326 21 347 1.46e-79 263
MS.gene035560.t1 MTR_7g117705 49.823 283 135 5 15 292 3 283 3.05e-78 255
MS.gene035560.t1 MTR_8g079940 44.311 334 147 9 15 327 23 338 2.61e-77 255
MS.gene035560.t1 MTR_3g086100 51.908 262 93 4 17 260 13 259 4.07e-75 250
MS.gene035560.t1 MTR_1g032570 47.863 234 109 6 76 297 1 233 1.08e-58 206
MS.gene035560.t1 MTR_3g102590 47.541 244 78 2 11 253 2 196 5.14e-58 194
MS.gene035560.t1 MTR_3g102590 46.667 240 78 2 11 249 2 192 1.48e-54 184
MS.gene035560.t1 MTR_3g086100 50.246 203 68 4 76 260 1 188 3.94e-50 181
MS.gene035560.t1 MTR_3g102600 63.710 124 45 0 17 140 8 131 1.94e-47 164
MS.gene035560.t1 MTR_3g102600 59.677 62 24 1 191 251 122 183 1.16e-16 79.0
MS.gene035560.t1 MTR_3g102600 63.710 124 45 0 17 140 8 131 4.55e-47 165
MS.gene035560.t1 MTR_3g102600 64.706 68 23 1 191 257 122 189 2.36e-20 90.5
MS.gene035560.t1 MTR_4g021760 34.274 248 138 3 16 263 10 232 7.00e-44 159
MS.gene035560.t1 MTR_4g021790 36.437 247 132 4 17 263 10 231 1.14e-43 157
MS.gene035560.t1 MTR_4g121020 36.842 304 156 7 96 375 6 297 6.51e-43 162
MS.gene035560.t1 MTR_0450s0040 36.154 260 133 5 21 279 8 235 9.32e-42 154
MS.gene035560.t1 MTR_4g021855 36.032 247 133 5 17 263 10 231 3.25e-41 151
MS.gene035560.t1 MTR_4g021845 37.021 235 121 6 16 249 9 217 6.68e-40 145
MS.gene035560.t1 MTR_6g045327 33.600 250 139 3 15 263 12 235 1.44e-39 149
MS.gene035560.t1 MTR_1g013160 50.000 148 71 2 79 223 1 148 8.68e-37 134
MS.gene035560.t1 MTR_3g102600 64.634 82 29 0 59 140 1 82 2.55e-31 119
MS.gene035560.t1 MTR_3g102600 61.017 59 22 1 194 251 76 134 1.76e-16 77.0
MS.gene035560.t1 MTR_3g102600 64.634 82 29 0 59 140 1 82 3.27e-31 120
MS.gene035560.t1 MTR_3g102600 64.706 68 23 1 191 257 73 140 1.22e-20 90.1
MS.gene035560.t1 MTR_8g105600 34.298 242 152 3 17 257 9 244 4.60e-31 128
MS.gene035560.t1 MTR_7g026400 34.685 222 128 3 47 266 17 223 2.08e-29 123
MS.gene035560.t1 MTR_5g055260 32.365 241 111 6 17 257 10 198 7.49e-29 115
MS.gene035560.t1 MTR_8g032710 25.769 260 172 4 16 255 16 274 3.98e-24 106
MS.gene035560.t1 MTR_3g092780 37.795 127 74 2 21 143 56 181 1.22e-23 105
MS.gene035560.t1 MTR_3g092780 37.795 127 74 2 21 143 56 181 1.31e-23 105
MS.gene035560.t1 MTR_3g092780 37.903 124 72 2 21 140 56 178 4.36e-23 104
MS.gene035560.t1 MTR_3g092780 37.903 124 72 2 21 140 56 178 4.63e-23 104
MS.gene035560.t1 MTR_7g118260 36.090 133 76 2 18 144 11 140 2.11e-22 102
MS.gene035560.t1 MTR_4g023980 33.540 161 83 4 63 223 35 171 3.78e-22 94.4
MS.gene035560.t1 MTR_7g118260 36.719 128 72 2 18 139 11 135 4.53e-22 100
MS.gene035560.t1 MTR_4g061360 39.231 130 75 2 14 139 81 210 6.45e-22 100
MS.gene035560.t1 MTR_8g024260 36.000 125 77 1 23 144 38 162 3.03e-21 98.6
MS.gene035560.t1 MTR_1g067110 38.525 122 72 1 21 139 85 206 6.87e-21 97.4
MS.gene035560.t1 MTR_1g067110 38.525 122 72 1 21 139 85 206 7.61e-21 97.4
MS.gene035560.t1 MTR_4g064730 61.842 76 26 1 182 257 116 188 9.70e-21 93.6
MS.gene035560.t1 MTR_8g093040 28.049 246 135 6 17 261 14 218 2.28e-20 92.0
MS.gene035560.t1 MTR_6g016850 32.836 134 87 2 17 148 9 141 9.87e-19 84.3
MS.gene035560.t1 MTR_7g089010 53.846 78 36 0 180 257 71 148 1.14e-18 87.4
MS.gene035560.t1 MTR_3g037390 33.333 123 79 1 21 140 24 146 7.40e-18 87.4
MS.gene035560.t1 MTR_4g108880 33.333 123 79 1 21 140 33 155 8.08e-18 87.4
MS.gene035560.t1 MTR_5g014040 39.516 124 71 2 17 139 14 134 5.45e-16 81.6
MS.gene035560.t1 MTR_5g014040 59.016 61 25 0 193 253 304 364 7.94e-15 77.8
MS.gene035560.t1 MTR_5g014040 39.516 124 71 2 17 139 14 134 5.97e-16 81.6
MS.gene035560.t1 MTR_5g014040 54.545 66 25 1 193 253 304 369 7.16e-13 71.6
MS.gene035560.t1 MTR_4g098870 62.069 58 22 0 196 253 306 363 5.65e-15 78.6
MS.gene035560.t1 MTR_4g098870 36.290 124 75 2 17 139 14 134 1.69e-14 77.0
MS.gene035560.t1 MTR_4g098870 62.069 58 22 0 196 253 326 383 5.74e-15 78.6
MS.gene035560.t1 MTR_4g098870 36.290 124 75 2 17 139 34 154 1.99e-14 76.6
MS.gene035560.t1 MTR_4g098870 62.069 58 22 0 196 253 306 363 5.92e-15 78.6
MS.gene035560.t1 MTR_4g098870 36.290 124 75 2 17 139 14 134 2.49e-14 76.3
MS.gene035560.t1 MTR_4g098870 62.069 58 22 0 196 253 326 383 6.50e-15 78.2
MS.gene035560.t1 MTR_4g098870 36.290 124 75 2 17 139 34 154 3.00e-14 76.3
MS.gene035560.t1 MTR_4g098870 60.656 61 24 0 193 253 342 402 6.73e-15 78.2
MS.gene035560.t1 MTR_4g098870 36.290 124 75 2 17 139 53 173 2.27e-14 76.6
MS.gene035560.t1 MTR_1g093080 51.471 68 32 1 193 259 204 271 1.78e-14 75.9
MS.gene035560.t1 MTR_4g113140 46.429 84 41 2 197 279 232 312 4.36e-14 75.1
MS.gene035560.t1 MTR_5g014040 54.545 66 25 1 193 253 264 329 6.39e-13 71.6
MS.gene035560.t1 MTR_4g086835 52.459 61 29 0 197 257 271 331 7.82e-13 71.2
MS.gene035560.t1 MTR_7g490310 30.612 147 79 3 23 148 11 155 1.16e-12 68.6
MS.gene035560.t1 MTR_7g115530 54.839 62 27 1 198 258 216 277 1.81e-12 68.9
MS.gene035560.t1 MTR_7g115530 54.839 62 27 1 198 258 216 277 3.06e-12 68.6
MS.gene035560.t1 MTR_4g078810 40.476 84 48 1 198 281 156 237 3.83e-12 68.9
MS.gene035560.t1 MTR_7g115530 54.839 62 27 1 198 258 216 277 4.37e-12 68.2
MS.gene035560.t1 MTR_7g115530 56.667 60 25 1 200 258 218 277 4.91e-12 68.6
MS.gene035560.t1 MTR_7g098250 46.835 79 40 2 196 272 258 336 5.96e-12 68.6
MS.gene035560.t1 MTR_5g017980 50.847 59 29 0 199 257 191 249 6.76e-12 67.4
MS.gene035560.t1 MTR_2g090675 41.333 75 43 1 178 252 140 213 6.92e-12 68.2
MS.gene035560.t1 MTR_5g017980 50.847 59 29 0 199 257 192 250 7.07e-12 67.4
MS.gene035560.t1 MTR_1g080330 51.562 64 30 1 196 258 171 234 7.80e-12 66.6
MS.gene035560.t1 MTR_1g080330 51.562 64 30 1 196 258 171 234 9.21e-12 67.4
MS.gene035560.t1 MTR_5g036480 26.163 172 107 4 21 172 16 187 1.87e-11 64.7
MS.gene035560.t1 MTR_4g086835 51.613 62 29 1 197 257 271 332 2.21e-11 66.6
MS.gene035560.t1 MTR_1874s0010 31.858 113 73 2 19 129 2 112 2.54e-11 64.3
MS.gene035560.t1 MTR_6g088950 29.545 132 85 2 47 175 34 160 2.76e-11 63.9
MS.gene035560.t1 MTR_5g054300 49.180 61 30 1 198 257 196 256 3.98e-11 65.5
MS.gene035560.t1 MTR_2g086450 53.333 60 27 1 196 254 41 100 6.80e-11 64.7
MS.gene035560.t1 MTR_5g041350 52.542 59 27 1 196 253 11 69 6.99e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035560.t1 AT2G25180 48.205 390 175 10 1 374 1 379 1.09e-94 302
MS.gene035560.t1 AT4G31920 51.562 320 131 6 17 325 13 319 2.49e-90 290
MS.gene035560.t1 AT5G58080 44.416 394 195 8 9 395 6 382 4.89e-81 268
MS.gene035560.t1 AT3G16857 47.987 298 128 3 15 288 31 325 4.58e-80 266
MS.gene035560.t1 AT3G16857 47.987 298 128 3 15 288 31 325 8.39e-80 266
MS.gene035560.t1 AT1G67710 55.253 257 96 5 16 260 5 254 4.29e-77 254
MS.gene035560.t1 AT2G01760 49.810 263 108 5 17 262 7 262 1.10e-66 222
MS.gene035560.t1 AT3G16857 47.471 257 108 3 56 288 2 255 5.63e-66 227
MS.gene035560.t1 AT2G01760 45.017 291 108 6 17 262 7 290 1.29e-60 207
MS.gene035560.t1 AT1G67710 53.571 196 73 4 76 260 1 189 3.28e-51 183
MS.gene035560.t1 AT5G49240 40.816 245 135 3 11 249 32 272 1.50e-50 176
MS.gene035560.t1 AT2G01760 50.000 204 78 5 76 262 1 197 1.96e-45 163
MS.gene035560.t1 AT3G62670 36.700 297 164 9 11 300 31 310 8.56e-40 151
MS.gene035560.t1 AT1G49190 29.967 307 168 7 17 300 30 312 1.83e-38 147
MS.gene035560.t1 AT1G67710 49.143 175 71 4 97 260 13 180 4.40e-37 144
MS.gene035560.t1 AT5G07210 29.710 276 153 4 21 262 16 284 2.21e-29 123
MS.gene035560.t1 AT5G07210 28.873 284 153 5 21 262 16 292 4.68e-27 116
MS.gene035560.t1 AT2G27070 30.182 275 158 6 21 263 16 288 5.25e-25 109
MS.gene035560.t1 AT5G24470 38.095 126 73 2 19 140 48 172 3.83e-23 103
MS.gene035560.t1 AT2G46790 36.806 144 83 4 3 139 16 158 3.99e-23 103
MS.gene035560.t1 AT2G46790 36.806 144 83 4 3 139 16 158 4.51e-23 103
MS.gene035560.t1 AT1G49190 36.800 125 79 0 17 141 30 154 1.02e-22 103
MS.gene035560.t1 AT5G59570 61.250 80 30 1 178 257 120 198 1.09e-22 99.4
MS.gene035560.t1 AT5G59570 61.250 80 30 1 178 257 120 198 1.09e-22 99.4
MS.gene035560.t1 AT5G60100 38.462 130 76 2 14 139 56 185 6.05e-22 99.0
MS.gene035560.t1 AT5G60100 38.462 130 76 2 14 139 56 185 6.05e-22 99.0
MS.gene035560.t1 AT5G60100 38.462 130 76 2 14 139 56 185 6.05e-22 99.0
MS.gene035560.t1 AT5G60100 38.462 130 76 2 14 139 56 185 1.10e-21 99.0
MS.gene035560.t1 AT5G60100 38.462 130 76 2 14 139 56 185 1.10e-21 99.0
MS.gene035560.t1 AT5G60100 38.462 130 76 2 14 139 56 185 1.10e-21 99.0
MS.gene035560.t1 AT5G60100 38.462 130 76 2 14 139 56 185 1.70e-21 98.6
MS.gene035560.t1 AT3G46640 62.667 75 27 1 183 257 128 201 1.83e-21 96.3
MS.gene035560.t1 AT3G46640 62.667 75 27 1 183 257 128 201 1.83e-21 96.3
MS.gene035560.t1 AT3G46640 62.667 75 27 1 183 257 128 201 2.37e-21 95.9
MS.gene035560.t1 AT5G05090 59.459 74 30 0 184 257 65 138 2.13e-20 91.7
MS.gene035560.t1 AT5G02810 36.885 122 74 1 21 139 78 199 9.60e-20 94.0
MS.gene035560.t1 AT3G10760 63.636 66 24 0 192 257 97 162 1.27e-19 90.9
MS.gene035560.t1 AT2G40970 63.077 65 24 0 193 257 98 162 4.74e-19 87.4
MS.gene035560.t1 AT1G68210 28.148 270 162 6 21 259 13 281 1.45e-18 90.5
MS.gene035560.t1 AT5G61380 34.959 123 77 1 21 140 19 141 1.19e-17 87.0
MS.gene035560.t1 AT5G44190 58.333 60 25 0 193 252 140 199 2.61e-15 78.6
MS.gene035560.t1 AT5G44190 58.333 60 25 0 193 252 140 199 3.02e-15 78.2
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.47e-14 75.9
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.46e-11 65.9
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.47e-14 75.9
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.46e-11 65.9
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.47e-14 75.9
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.46e-11 65.9
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.47e-14 75.9
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.46e-11 65.9
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.47e-14 75.9
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.46e-11 65.9
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 3.47e-14 75.9
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.46e-11 65.9
MS.gene035560.t1 AT1G25550 44.828 87 46 2 189 274 199 284 4.22e-14 74.3
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 4.59e-14 75.5
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.19e-11 66.2
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 4.59e-14 75.5
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.19e-11 66.2
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 4.59e-14 75.5
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.19e-11 66.2
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 4.59e-14 75.5
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.19e-11 66.2
MS.gene035560.t1 AT4G18020 37.063 143 81 3 15 150 12 152 4.59e-14 75.5
MS.gene035560.t1 AT4G18020 57.895 57 22 1 196 252 294 348 4.19e-11 66.2
MS.gene035560.t1 AT2G20570 48.101 79 38 1 174 252 130 205 2.38e-13 72.8
MS.gene035560.t1 AT2G03500 45.205 73 39 1 199 270 232 304 2.85e-13 72.8
MS.gene035560.t1 AT1G68670 44.304 79 42 2 182 259 198 275 3.02e-13 72.0
MS.gene035560.t1 AT4G37180 43.373 83 46 1 176 257 186 268 1.65e-12 69.7
MS.gene035560.t1 AT4G37180 43.373 83 46 1 176 257 193 275 1.82e-12 69.7
MS.gene035560.t1 AT3G13040 51.562 64 30 1 199 261 240 303 8.65e-12 68.2
MS.gene035560.t1 AT3G13040 51.562 64 30 1 199 261 240 303 8.65e-12 68.2
MS.gene035560.t1 AT3G13040 51.562 64 30 1 199 261 240 303 8.65e-12 68.2
MS.gene035560.t1 AT2G20570 62.500 48 18 0 205 252 174 221 1.08e-11 67.8
MS.gene035560.t1 AT3G25790 51.724 58 27 1 204 260 200 257 1.87e-11 66.6
MS.gene035560.t1 AT1G49560 45.070 71 38 1 199 268 191 261 3.88e-11 65.1
MS.gene035560.t1 AT1G79430 39.130 115 49 4 196 308 30 125 3.92e-11 65.5
MS.gene035560.t1 AT3G04030 53.333 60 27 1 196 254 41 100 4.51e-11 65.5
MS.gene035560.t1 AT3G12730 45.238 84 43 3 179 261 3 84 4.85e-11 63.5
MS.gene035560.t1 AT3G04030 53.333 60 27 1 196 254 41 100 4.88e-11 65.5

Find 107 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAACAATGATCAAATTTC+TGG 0.121819 8.4:+2037525 MS.gene035560:CDS
AGCTTGAAGCCATCCATTTC+TGG 0.175471 8.4:-2035864 None:intergenic
GTAGCAGGATTAAAAGATTT+TGG 0.205735 8.4:-2037453 None:intergenic
CCTCTCCCAGTTCTCACTTT+AGG 0.237398 8.4:+2037565 MS.gene035560:CDS
AAACATCGGCTGCTTGAAAC+TGG 0.273444 8.4:-2037160 None:intergenic
TCCGGTTGGTATGCGTGTTC+TGG 0.308828 8.4:+2034999 MS.gene035560:CDS
TGAGGCATCTGAGTCGTTAA+TGG 0.327980 8.4:-2037801 None:intergenic
AAGGGTTCAGTAGGTGGATA+TGG 0.338432 8.4:+2036976 MS.gene035560:CDS
TCGTTAATGGACTATTAGTT+TGG 0.342387 8.4:-2037788 None:intergenic
TTCGACACTTGCTGTTCCTT+CGG 0.359113 8.4:+2037608 MS.gene035560:CDS
CGTTAATGGACTATTAGTTT+GGG 0.359733 8.4:-2037787 None:intergenic
GAGCAACTGCTTGCTCTAGA+TGG 0.366890 8.4:+2037882 MS.gene035560:CDS
CTTCTCACATGCAAGTATAA+TGG 0.379037 8.4:+2035066 MS.gene035560:CDS
TTTGTAAATCACTCTTCTGT+TGG 0.381156 8.4:+2037414 MS.gene035560:CDS
TGAGCAAGTTGGACTTGAAA+TGG 0.392446 8.4:+2035890 MS.gene035560:CDS
GATAAATGTTCCGCTGAAAC+TGG 0.395287 8.4:-2037239 None:intergenic
AAAGCACTTGTATAATCAAT+TGG 0.414899 8.4:-2037285 None:intergenic
CACCTATCAACACATACTTC+AGG 0.430472 8.4:+2037387 MS.gene035560:CDS
GTTTCTATAGTTAATCTGCT+TGG 0.431269 8.4:+2036391 MS.gene035560:CDS
CTTACTTGACAATGTTGTAA+AGG 0.437919 8.4:+2037656 MS.gene035560:CDS
AGTGAAGATGTTACCGAAGA+AGG 0.438022 8.4:+2034973 MS.gene035560:CDS
CCACCGATGTTCGAACGCCC+TGG 0.438585 8.4:-2037209 None:intergenic
ACTGGATCCTCTCCAGCAAA+AGG 0.442603 8.4:+2036957 MS.gene035560:CDS
GTAAATCACTCTTCTGTTGG+AGG 0.444455 8.4:+2037417 MS.gene035560:CDS
ATCACTCAATGGAGAAGGAT+TGG 0.449366 8.4:-2037499 None:intergenic
TCTCAAACGACCGTCTAAAC+AGG 0.454349 8.4:+2036918 MS.gene035560:CDS
GCCAGAACACGCATACCAAC+CGG 0.455924 8.4:-2035000 None:intergenic
CTTAAGATTCTTCTAGATCC+AGG 0.456501 8.4:-2037015 None:intergenic
TAACAGTATCTCTGATGTCT+TGG 0.462540 8.4:-2037326 None:intergenic
TTCTGTTGGAGGTGCTTATG+TGG 0.465336 8.4:+2037428 MS.gene035560:CDS
ACCAACCGGAAAACCTTCTT+CGG 0.468377 8.4:-2034986 None:intergenic
TGAGAATAGTTGTGTGTCCT+TGG 0.476712 8.4:+2037911 MS.gene035560:CDS
TGATGTAAACATGCCAGAAA+TGG 0.478571 8.4:+2035851 MS.gene035560:CDS
CTTAAATTCATGTTACTAAG+TGG 0.478822 8.4:-2037261 None:intergenic
TTCTTCCTTCAACAATACCT+TGG 0.480661 8.4:+2037713 MS.gene035560:CDS
GATGGCTGTGGAGGATCGTT+TGG 0.483919 8.4:+2034948 None:intergenic
TTAGATGAACCATTTGTAGC+AGG 0.485124 8.4:-2037468 None:intergenic
TTGGTGTGATATAGTGTTGT+CGG 0.488347 8.4:+2036030 MS.gene035560:intron
GAGAAAGAAGATTGAAAGCA+AGG 0.491146 8.4:+2036162 MS.gene035560:CDS
CACCGATGTTCGAACGCCCT+GGG 0.491277 8.4:-2037208 None:intergenic
TGGTGTGATATAGTGTTGTC+GGG 0.492589 8.4:+2036031 MS.gene035560:intron
TGGAGGAGCTTAAGAACATA+TGG 0.492597 8.4:+2036128 MS.gene035560:CDS
GGCTAAAGTAGATGCTGCAC+TGG 0.493832 8.4:+2036939 MS.gene035560:CDS
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.4:+2036609 MS.gene035560:CDS
TTATAATTTGTACCTGCAGA+TGG 0.497679 8.4:-2036646 None:intergenic
ATCAAGGTATCGTTAGTGAT+GGG 0.497943 8.4:+2036191 MS.gene035560:CDS
TTAAGCTCCTCCAATCGAAT+CGG 0.500658 8.4:-2036118 None:intergenic
TTAGCTTACTAATGAAAGGC+AGG 0.500886 8.4:-2035914 None:intergenic
GAATAAAATTTCTCAACTTG+AGG 0.503061 8.4:-2037819 None:intergenic
AGAATCCAAGGTATTGTTGA+AGG 0.506294 8.4:-2037718 None:intergenic
TTCCCAGGGCGTTCGAACAT+CGG 0.508389 8.4:+2037206 MS.gene035560:CDS
CGGGACATGATGATAGAGAA+AGG 0.508606 8.4:+2036050 MS.gene035560:CDS
GAAAGCAAGGATCAAAATCA+AGG 0.515217 8.4:+2036175 MS.gene035560:CDS
TCAGACTAGTCTCAAAGTGC+TGG 0.521547 8.4:+2035035 MS.gene035560:CDS
TGGTTCCATCACTCTTGCAT+TGG 0.525055 8.4:-2037841 None:intergenic
CATTGCATCGCTTCCTCCGA+AGG 0.526768 8.4:-2037624 None:intergenic
TTTGTTAGCTTACTAATGAA+AGG 0.526782 8.4:-2035918 None:intergenic
AATCAAGGTATCGTTAGTGA+TGG 0.527040 8.4:+2036190 MS.gene035560:CDS
AGTCAAACCGATTCGATTGG+AGG 0.530770 8.4:+2036111 MS.gene035560:CDS
TTATTCCAATGCAAGAGTGA+TGG 0.531742 8.4:+2037836 MS.gene035560:CDS
CGTGACTTGATGAATGTTGA+AGG 0.537018 8.4:+2036599 MS.gene035560:CDS
CAGCAAAAGGGTTCAGTAGG+TGG 0.537224 8.4:+2036970 MS.gene035560:CDS
TGGAAGCGCTTGAATTGTTG+AGG 0.538411 8.4:+2035796 MS.gene035560:CDS
ATTCTGGTTGATGATGGCTG+TGG 0.540952 8.4:+2034936 None:intergenic
CTGGATCCTCTCCAGCAAAA+GGG 0.541917 8.4:+2036958 MS.gene035560:CDS
TGAGACTGAAGTGGAGGGAC+TGG 0.545626 8.4:-2037129 None:intergenic
TTCAAGCTCCTTGAGCAAGT+TGG 0.547513 8.4:+2035879 MS.gene035560:CDS
GACACTTGCTGTTCCTTCGG+AGG 0.551078 8.4:+2037611 MS.gene035560:CDS
AAATGTAGCTAGCCATCTGC+AGG 0.551114 8.4:+2036634 MS.gene035560:CDS
TTGTTAAAATGATCACTCAA+TGG 0.551340 8.4:-2037510 None:intergenic
TCTAGTCAAACCGATTCGAT+TGG 0.552115 8.4:+2036108 MS.gene035560:CDS
AACATCGGCTGCTTGAAACT+GGG 0.553633 8.4:-2037159 None:intergenic
GGAAGCGCTTGAATTGTTGA+GGG 0.554887 8.4:+2035797 MS.gene035560:CDS
ATCGTTAGTGATGGGGTATG+TGG 0.558412 8.4:+2036199 MS.gene035560:CDS
CTGGTTGATGATGGCTGTGG+AGG 0.558423 8.4:+2034939 None:intergenic
TTTCAAGTCCAACTTGCTCA+AGG 0.564395 8.4:-2035887 None:intergenic
TCTACTTTAGCCTGTTTAGA+CGG 0.566286 8.4:-2036928 None:intergenic
TTAACTTATTGTGTCATCCA+AGG 0.570842 8.4:-2037928 None:intergenic
CTCCTGAAGTATGTGTTGAT+AGG 0.571194 8.4:-2037389 None:intergenic
AAGAAAGGAACAAAGTGAGG+AGG 0.573387 8.4:+2036276 MS.gene035560:CDS
ATAAGAAGCAAGAGAATCCA+AGG 0.574630 8.4:-2037730 None:intergenic
AAATGATCACTCAATGGAGA+AGG 0.574716 8.4:-2037504 None:intergenic
TAATGAAGACACTAGTTCCA+TGG 0.575646 8.4:+2037752 MS.gene035560:CDS
GATTCTTCTAGATCCAGGTA+AGG 0.579926 8.4:-2037010 None:intergenic
CCAGGGCGTTCGAACATCGG+TGG 0.583592 8.4:+2037209 MS.gene035560:CDS
GTCATATAAACTCACTGTCA+AGG 0.583894 8.4:-2036414 None:intergenic
GAAGCGATGCAATGTCAAGA+TGG 0.589336 8.4:+2037633 MS.gene035560:CDS
GGGACATGATGATAGAGAAA+GGG 0.593987 8.4:+2036051 MS.gene035560:CDS
CTTTCACTTGCAGAAAACAT+CGG 0.600525 8.4:-2037174 None:intergenic
TTGGGGTACACTTTGTACCA+TGG 0.600741 8.4:-2037769 None:intergenic
CCTAAAGTGAGAACTGGGAG+AGG 0.607515 8.4:-2037565 None:intergenic
GCTAACAAGAAGAAAATGCA+TGG 0.607753 8.4:+2036247 MS.gene035560:CDS
TACTACAACTACGAATTCAG+TGG 0.615187 8.4:+2035776 MS.gene035560:CDS
CTCCAGCAAAAGGGTTCAGT+AGG 0.616386 8.4:+2036967 MS.gene035560:CDS
ATGACGAGGAACACTCAACT+CGG 0.622969 8.4:+2036323 MS.gene035560:CDS
ATGTCACTAGATGCATATGT+TGG 0.628893 8.4:-2037048 None:intergenic
GTTAATGGACTATTAGTTTG+GGG 0.635315 8.4:-2037786 None:intergenic
GATAGAGAAAGGGTGATGAA+AGG 0.639508 8.4:+2036061 MS.gene035560:CDS
TCAAGGTATCGTTAGTGATG+GGG 0.646944 8.4:+2036192 MS.gene035560:CDS
GTGATGAAAGGTGTGATGAA+AGG 0.648487 8.4:+2036073 MS.gene035560:CDS
GTAAACATGCCAGAAATGGA+TGG 0.659081 8.4:+2035855 MS.gene035560:CDS
GTGACTTGATGAATGTTGAA+GGG 0.669266 8.4:+2036600 MS.gene035560:CDS
TGGAAATTAACCAGTTTCAG+CGG 0.669997 8.4:+2037229 MS.gene035560:CDS
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.4:+2036610 MS.gene035560:CDS
TCATTATCCCAAACAAAACG+CGG 0.696392 8.4:-2036352 None:intergenic
TACTGATGAAGAGAATGACG+AGG 0.706123 8.4:+2036309 MS.gene035560:CDS
GCAAGAGTGATGGAACCACG+TGG 0.841092 8.4:+2037846 MS.gene035560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAAAGGGAAAAAAA+GGG - chr8.4:2035220-2035239 None:intergenic 15.0%
!! AATTAATCATATTTGCAAAA+AGG - chr8.4:2036732-2036751 None:intergenic 15.0%
!! CAAATATGATTAATTTAAAG+GGG + chr8.4:2036737-2036756 MS.gene035560:intron 15.0%
!! CCGATAAAATAAAATAAAAA+GGG - chr8.4:2035230-2035249 None:intergenic 15.0%
!! GCAAATATGATTAATTTAAA+GGG + chr8.4:2036736-2036755 MS.gene035560:intron 15.0%
!! TAAAATAAAAAGGGAAAAAA+AGG - chr8.4:2035221-2035240 None:intergenic 15.0%
!! TGCAAATATGATTAATTTAA+AGG + chr8.4:2036735-2036754 MS.gene035560:intron 15.0%
!!! CCCTTTTTATTTTATTTTAT+CGG + chr8.4:2035227-2035246 MS.gene035560:intron 15.0%
!!! TGAGATACATTTAAAATAAA+AGG - chr8.4:2035722-2035741 None:intergenic 15.0%
!! AAGATGTAAATTTCAACTTT+AGG - chr8.4:2035569-2035588 None:intergenic 20.0%
!! ATGAAGAAACTACATATATT+AGG - chr8.4:2036547-2036566 None:intergenic 20.0%
!! CAATCATGTAAACATATAAT+TGG + chr8.4:2035173-2035192 MS.gene035560:intron 20.0%
!! GCCGATAAAATAAAATAAAA+AGG - chr8.4:2035231-2035250 None:intergenic 20.0%
!! TACTATCTAAATATAAGAAG+TGG - chr8.4:2035987-2036006 None:intergenic 20.0%
!! TGATGATTGATACTTAAATA+GGG + chr8.4:2036510-2036529 MS.gene035560:intron 20.0%
!! TTATAAGCTATAAGAATGTT+CGG + chr8.4:2035319-2035338 MS.gene035560:intron 20.0%
!! TTTAACAATGATCAAATTTC+TGG + chr8.4:2037525-2037544 MS.gene035560:CDS 20.0%
!! TTTAAGTATCAATCATCAAA+TGG - chr8.4:2036508-2036527 None:intergenic 20.0%
!!! AGTGATTATATTAGTTTCTA+GGG + chr8.4:2035276-2035295 MS.gene035560:intron 20.0%
!!! ATTCAACATTTGCTATTATT+GGG + chr8.4:2035666-2035685 MS.gene035560:intron 20.0%
!!! TTAGTGATGATTTTTTCTTT+TGG + chr8.4:2036011-2036030 MS.gene035560:intron 20.0%
!!! TTGATGATTGATACTTAAAT+AGG + chr8.4:2036509-2036528 MS.gene035560:intron 20.0%
! ACACATGTTAAGGAAAATTA+AGG - chr8.4:2035944-2035963 None:intergenic 25.0%
! ATTGAACGAATGAAAAAGAA+AGG - chr8.4:2035099-2035118 None:intergenic 25.0%
! CTTAAATTCATGTTACTAAG+TGG - chr8.4:2037264-2037283 None:intergenic 25.0%
! GAATAAAATTTCTCAACTTG+AGG - chr8.4:2037822-2037841 None:intergenic 25.0%
! TAAGTGTGATGTGTATATAT+AGG + chr8.4:2035409-2035428 MS.gene035560:intron 25.0%
! TAATCATATTTGCAAAAAGG+CGG - chr8.4:2036729-2036748 None:intergenic 25.0%
! TATAGGTTATATAAGTCTTC+CGG + chr8.4:2035426-2035445 MS.gene035560:intron 25.0%
! TTGTTAAAATGATCACTCAA+TGG - chr8.4:2037513-2037532 None:intergenic 25.0%
!! AAAGCACTTGTATAATCAAT+TGG - chr8.4:2037288-2037307 None:intergenic 25.0%
!! AAATTCATTCTTTTGCAAGA+TGG + chr8.4:2035522-2035541 MS.gene035560:intron 25.0%
!! GAGTGATTATATTAGTTTCT+AGG + chr8.4:2035275-2035294 MS.gene035560:intron 25.0%
!! TTTGTTAGCTTACTAATGAA+AGG - chr8.4:2035921-2035940 None:intergenic 25.0%
!!! AGTCTGAAACTTTTTACTAA+AGG - chr8.4:2036681-2036700 None:intergenic 25.0%
!!! ATCAAGTCACGTATTTTTTT+AGG - chr8.4:2036590-2036609 None:intergenic 25.0%
!!! ATTACTACTTTTTATTGTGC+CGG + chr8.4:2035595-2035614 MS.gene035560:intron 25.0%
!!! GATTCAACATTTGCTATTAT+TGG + chr8.4:2035665-2035684 MS.gene035560:intron 25.0%
!!! GTCTGAAACTTTTTACTAAA+GGG - chr8.4:2036680-2036699 None:intergenic 25.0%
!!! TCTGAAACTTTTTACTAAAG+GGG - chr8.4:2036679-2036698 None:intergenic 25.0%
AAAAAGAAAGGAACAAAGTG+AGG + chr8.4:2036273-2036292 MS.gene035560:CDS 30.0%
ACACATGTGTTAATTAGCAT+CGG + chr8.4:2035346-2035365 MS.gene035560:intron 30.0%
ACAGTGAGTTTATATGACAA+TGG + chr8.4:2036419-2036438 MS.gene035560:intron 30.0%
AGAGAATGTAAGTGATTGAA+TGG - chr8.4:2035379-2035398 None:intergenic 30.0%
ATACGCTTGCATACAAATAA+TGG + chr8.4:2036777-2036796 MS.gene035560:intron 30.0%
CTTACTTGACAATGTTGTAA+AGG + chr8.4:2037656-2037675 MS.gene035560:CDS 30.0%
GAGAATGTAAGTGATTGAAT+GGG - chr8.4:2035378-2035397 None:intergenic 30.0%
GCTGAATAATACACATGTTA+AGG - chr8.4:2035954-2035973 None:intergenic 30.0%
GTTGAATCAAAATAGTGATG+AGG - chr8.4:2035653-2035672 None:intergenic 30.0%
GTTTCTATAGTTAATCTGCT+TGG + chr8.4:2036391-2036410 MS.gene035560:CDS 30.0%
TGAATCACTACTTTGATTCT+TGG + chr8.4:2035743-2035762 MS.gene035560:intron 30.0%
TTAACTTATTGTGTCATCCA+AGG - chr8.4:2037931-2037950 None:intergenic 30.0%
TTCTTCATCTATTATTGCAG+AGG + chr8.4:2036559-2036578 MS.gene035560:intron 30.0%
TTTGTAAATCACTCTTCTGT+TGG + chr8.4:2037414-2037433 MS.gene035560:CDS 30.0%
! GTAGCAGGATTAAAAGATTT+TGG - chr8.4:2037456-2037475 None:intergenic 30.0%
! GTTAGCAATATTTTCAGATG+AGG - chr8.4:2036233-2036252 None:intergenic 30.0%
! TCTTTTAATCCTGCTACAAA+TGG + chr8.4:2037459-2037478 MS.gene035560:CDS 30.0%
! TTATAATTTGTACCTGCAGA+TGG - chr8.4:2036649-2036668 None:intergenic 30.0%
!! CGTTAATGGACTATTAGTTT+GGG - chr8.4:2037790-2037809 None:intergenic 30.0%
!! GTTAATGGACTATTAGTTTG+GGG - chr8.4:2037789-2037808 None:intergenic 30.0%
!! TCGTTAATGGACTATTAGTT+TGG - chr8.4:2037791-2037810 None:intergenic 30.0%
AAATGATCACTCAATGGAGA+AGG - chr8.4:2037507-2037526 None:intergenic 35.0%
AATGCATGGACGAAAAAGAA+AGG + chr8.4:2036261-2036280 MS.gene035560:CDS 35.0%
ATAAGAAGCAAGAGAATCCA+AGG - chr8.4:2037733-2037752 None:intergenic 35.0%
ATGTCACTAGATGCATATGT+TGG - chr8.4:2037051-2037070 None:intergenic 35.0%
CTTAAGATTCTTCTAGATCC+AGG - chr8.4:2037018-2037037 None:intergenic 35.0%
CTTCTCACATGCAAGTATAA+TGG + chr8.4:2035066-2035085 MS.gene035560:CDS 35.0%
CTTTCACTTGCAGAAAACAT+CGG - chr8.4:2037177-2037196 None:intergenic 35.0%
GAAAGCAAGGATCAAAATCA+AGG + chr8.4:2036175-2036194 MS.gene035560:CDS 35.0%
GAGAAAGAAGATTGAAAGCA+AGG + chr8.4:2036162-2036181 MS.gene035560:CDS 35.0%
GCTAACAAGAAGAAAATGCA+TGG + chr8.4:2036247-2036266 MS.gene035560:CDS 35.0%
GGTTTCTATTACTAATGTGC+CGG - chr8.4:2035617-2035636 None:intergenic 35.0%
GTCATATAAACTCACTGTCA+AGG - chr8.4:2036417-2036436 None:intergenic 35.0%
GTGACTTGATGAATGTTGAA+GGG + chr8.4:2036600-2036619 MS.gene035560:CDS 35.0%
TAACAGTATCTCTGATGTCT+TGG - chr8.4:2037329-2037348 None:intergenic 35.0%
TAATGAAGACACTAGTTCCA+TGG + chr8.4:2037752-2037771 MS.gene035560:CDS 35.0%
TACTACAACTACGAATTCAG+TGG + chr8.4:2035776-2035795 MS.gene035560:CDS 35.0%
TCATTATCCCAAACAAAACG+CGG - chr8.4:2036355-2036374 None:intergenic 35.0%
TCTACTTTAGCCTGTTTAGA+CGG - chr8.4:2036931-2036950 None:intergenic 35.0%
TGATGAGGTTTATAGTGATG+AGG - chr8.4:2035638-2035657 None:intergenic 35.0%
TGATGTAAACATGCCAGAAA+TGG + chr8.4:2035851-2035870 MS.gene035560:CDS 35.0%
TGGAAATTAACCAGTTTCAG+CGG + chr8.4:2037229-2037248 MS.gene035560:CDS 35.0%
TTAGATGAACCATTTGTAGC+AGG - chr8.4:2037471-2037490 None:intergenic 35.0%
TTAGCTTACTAATGAAAGGC+AGG - chr8.4:2035917-2035936 None:intergenic 35.0%
TTATTCCAATGCAAGAGTGA+TGG + chr8.4:2037836-2037855 MS.gene035560:CDS 35.0%
TTCTTCCTTCAACAATACCT+TGG + chr8.4:2037713-2037732 MS.gene035560:CDS 35.0%
TTGGTGTGATATAGTGTTGT+CGG + chr8.4:2036030-2036049 MS.gene035560:intron 35.0%
! ACTTTTCCTAAAGTGAGAAC+TGG - chr8.4:2037574-2037593 None:intergenic 35.0%
! AGTGCTTTTACAGTTTCTTC+TGG + chr8.4:2037300-2037319 MS.gene035560:CDS 35.0%
! AGTGTCGAAGAAAAATCAAC+TGG - chr8.4:2037597-2037616 None:intergenic 35.0%
! CATCTAGTGACATGTTTTCT+CGG + chr8.4:2037058-2037077 MS.gene035560:CDS 35.0%
! CTTTTCCTAAAGTGAGAACT+GGG - chr8.4:2037573-2037592 None:intergenic 35.0%
!! AATCAAGGTATCGTTAGTGA+TGG + chr8.4:2036190-2036209 MS.gene035560:CDS 35.0%
!! AGAATCCAAGGTATTGTTGA+AGG - chr8.4:2037721-2037740 None:intergenic 35.0%
!! ATCAAGGTATCGTTAGTGAT+GGG + chr8.4:2036191-2036210 MS.gene035560:CDS 35.0%
AAAGGCTTCCTGCGTTATAA+AGG - chr8.4:2035704-2035723 None:intergenic 40.0%
AAGAAAGGAACAAAGTGAGG+AGG + chr8.4:2036276-2036295 MS.gene035560:CDS 40.0%
ACCTATATGTAGCTGATGTG+AGG - chr8.4:2035456-2035475 None:intergenic 40.0%
AGTGAAGATGTTACCGAAGA+AGG + chr8.4:2034973-2034992 MS.gene035560:CDS 40.0%
ATCACTCAATGGAGAAGGAT+TGG - chr8.4:2037502-2037521 None:intergenic 40.0%
CACCTATCAACACATACTTC+AGG + chr8.4:2037387-2037406 MS.gene035560:CDS 40.0%
CCTATATGTAGCTGATGTGA+GGG - chr8.4:2035455-2035474 None:intergenic 40.0%
CGTGACTTGATGAATGTTGA+AGG + chr8.4:2036599-2036618 MS.gene035560:CDS 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.4:2036610-2036629 MS.gene035560:CDS 40.0%
GATAAATGTTCCGCTGAAAC+TGG - chr8.4:2037242-2037261 None:intergenic 40.0%
GATAGAGAAAGGGTGATGAA+AGG + chr8.4:2036061-2036080 MS.gene035560:CDS 40.0%
GATTCTTCTAGATCCAGGTA+AGG - chr8.4:2037013-2037032 None:intergenic 40.0%
GCTATTATTGGGTGAATCGA+CGG + chr8.4:2035677-2035696 MS.gene035560:intron 40.0%
GGGACATGATGATAGAGAAA+GGG + chr8.4:2036051-2036070 MS.gene035560:CDS 40.0%
GGGTTTGAACAATGACTTCT+TGG + chr8.4:2035296-2035315 MS.gene035560:intron 40.0%
GTAAACATGCCAGAAATGGA+TGG + chr8.4:2035855-2035874 MS.gene035560:CDS 40.0%
GTAAATCACTCTTCTGTTGG+AGG + chr8.4:2037417-2037436 MS.gene035560:CDS 40.0%
TACTGATGAAGAGAATGACG+AGG + chr8.4:2036309-2036328 MS.gene035560:CDS 40.0%
TCAAAAAAGCAATCACCACG+TGG - chr8.4:2037864-2037883 None:intergenic 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.4:2036609-2036628 MS.gene035560:CDS 40.0%
TGAGAATAGTTGTGTGTCCT+TGG + chr8.4:2037911-2037930 MS.gene035560:CDS 40.0%
TGGAGGAGCTTAAGAACATA+TGG + chr8.4:2036128-2036147 MS.gene035560:CDS 40.0%
TGGTGTGATATAGTGTTGTC+GGG + chr8.4:2036031-2036050 MS.gene035560:intron 40.0%
TTAAGCTCCTCCAATCGAAT+CGG - chr8.4:2036121-2036140 None:intergenic 40.0%
! CTCCTGAAGTATGTGTTGAT+AGG - chr8.4:2037392-2037411 None:intergenic 40.0%
! GAAAGCTTGAGTTTTTCCCA+GGG + chr8.4:2037192-2037211 MS.gene035560:CDS 40.0%
! GTGATGAAAGGTGTGATGAA+AGG + chr8.4:2036073-2036092 MS.gene035560:CDS 40.0%
! TGAAAGCTTGAGTTTTTCCC+AGG + chr8.4:2037191-2037210 MS.gene035560:CDS 40.0%
! TGAGCAAGTTGGACTTGAAA+TGG + chr8.4:2035890-2035909 MS.gene035560:CDS 40.0%
! TGTTACCGAAGAAGGTTTTC+CGG + chr8.4:2034981-2035000 MS.gene035560:CDS 40.0%
! TTTCAAGTCCAACTTGCTCA+AGG - chr8.4:2035890-2035909 None:intergenic 40.0%
!! TCAAGGTATCGTTAGTGATG+GGG + chr8.4:2036192-2036211 MS.gene035560:CDS 40.0%
!! TCTAGTCAAACCGATTCGAT+TGG + chr8.4:2036108-2036127 MS.gene035560:CDS 40.0%
!!! ATGTTTTGAGACTGAAGTGG+AGG - chr8.4:2037138-2037157 None:intergenic 40.0%
!!! GGAATGTTTTGAGACTGAAG+TGG - chr8.4:2037141-2037160 None:intergenic 40.0%
!!! TGTTTTGAGACTGAAGTGGA+GGG - chr8.4:2037137-2037156 None:intergenic 40.0%
AAATGTAGCTAGCCATCTGC+AGG + chr8.4:2036634-2036653 MS.gene035560:CDS 45.0%
AAGGGTTCAGTAGGTGGATA+TGG + chr8.4:2036976-2036995 MS.gene035560:CDS 45.0%
ACCAACCGGAAAACCTTCTT+CGG - chr8.4:2034989-2035008 None:intergenic 45.0%
AGCTTGAAGCCATCCATTTC+TGG - chr8.4:2035867-2035886 None:intergenic 45.0%
ATGACGAGGAACACTCAACT+CGG + chr8.4:2036323-2036342 MS.gene035560:CDS 45.0%
CCCTCACATCAGCTACATAT+AGG + chr8.4:2035452-2035471 MS.gene035560:intron 45.0%
CGGGACATGATGATAGAGAA+AGG + chr8.4:2036050-2036069 MS.gene035560:CDS 45.0%
GAAGCGATGCAATGTCAAGA+TGG + chr8.4:2037633-2037652 MS.gene035560:CDS 45.0%
TCTCAAACGACCGTCTAAAC+AGG + chr8.4:2036918-2036937 MS.gene035560:CDS 45.0%
TGGTTCCATCACTCTTGCAT+TGG - chr8.4:2037844-2037863 None:intergenic 45.0%
TTCAAGCTCCTTGAGCAAGT+TGG + chr8.4:2035879-2035898 MS.gene035560:CDS 45.0%
TTGGGGTACACTTTGTACCA+TGG - chr8.4:2037772-2037791 None:intergenic 45.0%
! AAACATCGGCTGCTTGAAAC+TGG - chr8.4:2037163-2037182 None:intergenic 45.0%
! AACATCGGCTGCTTGAAACT+GGG - chr8.4:2037162-2037181 None:intergenic 45.0%
! ATCGTTAGTGATGGGGTATG+TGG + chr8.4:2036199-2036218 MS.gene035560:CDS 45.0%
! TGAGGCATCTGAGTCGTTAA+TGG - chr8.4:2037804-2037823 None:intergenic 45.0%
!! AGTCAAACCGATTCGATTGG+AGG + chr8.4:2036111-2036130 MS.gene035560:CDS 45.0%
!! GGAAGCGCTTGAATTGTTGA+GGG + chr8.4:2035797-2035816 MS.gene035560:CDS 45.0%
!! TCAGACTAGTCTCAAAGTGC+TGG + chr8.4:2035035-2035054 MS.gene035560:CDS 45.0%
!! TGGAAGCGCTTGAATTGTTG+AGG + chr8.4:2035796-2035815 MS.gene035560:CDS 45.0%
!! TTCGACACTTGCTGTTCCTT+CGG + chr8.4:2037608-2037627 MS.gene035560:CDS 45.0%
!! TTCTGTTGGAGGTGCTTATG+TGG + chr8.4:2037428-2037447 MS.gene035560:CDS 45.0%
!!! GAAGAAGCCGCGTTTTGTTT+GGG + chr8.4:2036345-2036364 MS.gene035560:CDS 45.0%
ACTGGATCCTCTCCAGCAAA+AGG + chr8.4:2036957-2036976 MS.gene035560:CDS 50.0%
CAGCAAAAGGGTTCAGTAGG+TGG + chr8.4:2036970-2036989 MS.gene035560:CDS 50.0%
CCTAAAGTGAGAACTGGGAG+AGG - chr8.4:2037568-2037587 None:intergenic 50.0%
CCTCTCCCAGTTCTCACTTT+AGG + chr8.4:2037565-2037584 MS.gene035560:CDS 50.0%
CTCCAGCAAAAGGGTTCAGT+AGG + chr8.4:2036967-2036986 MS.gene035560:CDS 50.0%
CTGGATCCTCTCCAGCAAAA+GGG + chr8.4:2036958-2036977 MS.gene035560:CDS 50.0%
GGCTAAAGTAGATGCTGCAC+TGG + chr8.4:2036939-2036958 MS.gene035560:CDS 50.0%
TCGACGGTCCTTTATAACGC+AGG + chr8.4:2035693-2035712 MS.gene035560:intron 50.0%
! ACCGAAGAAGGTTTTCCGGT+TGG + chr8.4:2034985-2035004 MS.gene035560:CDS 50.0%
! ACTGAACCCTTTTGCTGGAG+AGG - chr8.4:2036967-2036986 None:intergenic 50.0%
! CACCTACTGAACCCTTTTGC+TGG - chr8.4:2036972-2036991 None:intergenic 50.0%
! GAGCAACTGCTTGCTCTAGA+TGG + chr8.4:2037882-2037901 MS.gene035560:CDS 50.0%
! GGAGATTTTCGCACCTTACC+TGG + chr8.4:2036997-2037016 MS.gene035560:CDS 50.0%
!! GGAAGAAGCCGCGTTTTGTT+TGG + chr8.4:2036344-2036363 MS.gene035560:CDS 50.0%
CATTGCATCGCTTCCTCCGA+AGG - chr8.4:2037627-2037646 None:intergenic 55.0%
GCCAGAACACGCATACCAAC+CGG - chr8.4:2035003-2035022 None:intergenic 55.0%
GTAGCTGATGTGAGGGACAC+CGG - chr8.4:2035448-2035467 None:intergenic 55.0%
TGAGACTGAAGTGGAGGGAC+TGG - chr8.4:2037132-2037151 None:intergenic 55.0%
! GCAAGAGTGATGGAACCACG+TGG + chr8.4:2037846-2037865 MS.gene035560:CDS 55.0%
! TCCGGTTGGTATGCGTGTTC+TGG + chr8.4:2034999-2035018 MS.gene035560:CDS 55.0%
! TTCCCAGGGCGTTCGAACAT+CGG + chr8.4:2037206-2037225 MS.gene035560:CDS 55.0%
!! GACACTTGCTGTTCCTTCGG+AGG + chr8.4:2037611-2037630 MS.gene035560:CDS 55.0%
CACCGATGTTCGAACGCCCT+GGG - chr8.4:2037211-2037230 None:intergenic 60.0%
CCACCGATGTTCGAACGCCC+TGG - chr8.4:2037212-2037231 None:intergenic 65.0%
! CCAGGGCGTTCGAACATCGG+TGG + chr8.4:2037209-2037228 MS.gene035560:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 2034949 2037950 2034949 ID=MS.gene035560
chr8.4 mRNA 2034949 2037950 2034949 ID=MS.gene035560.t1;Parent=MS.gene035560
chr8.4 exon 2034949 2035087 2034949 ID=MS.gene035560.t1.exon1;Parent=MS.gene035560.t1
chr8.4 CDS 2034949 2035087 2034949 ID=cds.MS.gene035560.t1;Parent=MS.gene035560.t1
chr8.4 exon 2035775 2035927 2035775 ID=MS.gene035560.t1.exon2;Parent=MS.gene035560.t1
chr8.4 CDS 2035775 2035927 2035775 ID=cds.MS.gene035560.t1;Parent=MS.gene035560.t1
chr8.4 exon 2036044 2036421 2036044 ID=MS.gene035560.t1.exon3;Parent=MS.gene035560.t1
chr8.4 CDS 2036044 2036421 2036044 ID=cds.MS.gene035560.t1;Parent=MS.gene035560.t1
chr8.4 exon 2036579 2036655 2036579 ID=MS.gene035560.t1.exon4;Parent=MS.gene035560.t1
chr8.4 CDS 2036579 2036655 2036579 ID=cds.MS.gene035560.t1;Parent=MS.gene035560.t1
chr8.4 exon 2036904 2037950 2036904 ID=MS.gene035560.t1.exon5;Parent=MS.gene035560.t1
chr8.4 CDS 2036904 2037950 2036904 ID=cds.MS.gene035560.t1;Parent=MS.gene035560.t1
Gene Sequence

>MS.gene035560

ATGGCTGTGGAGGATCGTTTGGTGAGTGAAGATGTTACCGAAGAAGGTTTTCCGGTTGGTATGCGTGTTCTGGCTGTTGATGACGATCAGACTAGTCTCAAAGTGCTGGAGAAGCATCTTCTCACATGCAAGTATAATGGTTCGTTGCCTTTCTTTTTCATTCGTTCAATTTGATTCTGCTTTGATGATGCTTTCATGTTTTCACTTACTATTCTTCTCTCATGCAATCATGTAAACATATAATTGGAAGTATCTTTGATATAGCAAACCCTTTTTTTCCCTTTTTATTTTATTTTATCGGCAAGTGTTAGTTGTTAGTGATTAGTGAGTGATTATATTAGTTTCTAGGGTTTGAACAATGACTTCTTGGTTATAAGCTATAAGAATGTTCGGACTCACACATGTGTTAATTAGCATCGGACACAACCCATTCAATCACTTACATTCTCTCAAATTATTATAAGTGTGATGTGTATATATAGGTTATATAAGTCTTCCGGTGTCCCTCACATCAGCTACATATAGGTTATATATACACACATCAGTCTACAGTTTTCATGAATTTAGATACAAAAATTCATTCTTTTGCAAGATGGCTGTGTTTTAATTTCTATTATCCTAAAGTTGAAATTTACATCTTTTAAAGATTACTACTTTTTATTGTGCCGGCACATTAGTAATAGAAACCTCATCACTATAAACCTCATCACTATTTTGATTCAACATTTGCTATTATTGGGTGAATCGACGGTCCTTTATAACGCAGGAAGCCTTTTATTTTAAATGTATCTCACTGAATCACTACTTTGATTCTTGGTTGTTGCAGTTACTACAACTACGAATTCAGTGGAAGCGCTTGAATTGTTGAGGGAAAAGAGAAGTATGTTTGATCTTATAATTAGTGATGTAAACATGCCAGAAATGGATGGCTTCAAGCTCCTTGAGCAAGTTGGACTTGAAATGGACCTGCCTTTCATTAGTAAGCTAACAAACCTTAATTTTCCTTAACATGTGTATTATTCAGCTAATTAAGATCCACTTCTTATATTTAGATAGTATCTTTTAGTGATGATTTTTTCTTTTGGTGTGATATAGTGTTGTCGGGACATGATGATAGAGAAAGGGTGATGAAAGGTGTGATGAAAGGCGCTTGCGACTATCTAGTCAAACCGATTCGATTGGAGGAGCTTAAGAACATATGGCAACATGTAGTGAGAAAGAAGATTGAAAGCAAGGATCAAAATCAAGGTATCGTTAGTGATGGGGTATGTGGTCAAGACACCTCATCTGAAAATATTGCTAACAAGAAGAAAATGCATGGACGAAAAAGAAAGGAACAAAGTGAGGAGGAAGAAGCAGATACTGATGAAGAGAATGACGAGGAACACTCAACTCGGAAGAAGCCGCGTTTTGTTTGGGATAATGAGTTGCACAAAAAATTTGTTTCTATAGTTAATCTGCTTGGCCTTGACAGTGAGTTTATATGACAATGGTTTTTTTTTATTTCTTTTTACTTTGTTAACTTGCTTGACTTGAAAACATGAAACTTAATAAGACCATTTGATGATTGATACTTAAATAGGGATATTATTGTTCCTAATATATGTAGTTTCTTCATCTATTATTGCAGAGGCTTATCCTAAAAAAATACGTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTAGCTAGCCATCTGCAGGTACAAATTATAAATAACTTCCCCTTTAGTAAAAAGTTTCAGACTAGTTGATCATTAAAGTATTTGATCACCGCCTTTTTGCAAATATGATTAATTTAAAGGGGATAGTTTACTTTTAAACATACGCTTGCATACAAATAATGGTGTGAAATTTTTTTAGATCAGTTTTTCATTGAATTTCAACAAGCTAGTGTATGAAACAAAAGTATGAAAACAGTGTGTAAGCTCATTCTCGTTTTCGATTGCAGAAATACAGACTTAGTCTCAAACGACCGTCTAAACAGGCTAAAGTAGATGCTGCACTGGATCCTCTCCAGCAAAAGGGTTCAGTAGGTGGATATGGAGATTTTCGCACCTTACCTGGATCTAGAAGAATCTTAAGTAGCACACTGCCAACATATGCATCTAGTGACATGTTTTCTCGGCTGAACGCATCATCTAGTTTGAACTTTAGACGAATGAGTTCTTCTGCACCAGTCCCTCCACTTCAGTCTCAAAACATTCCCAGTTTCAAGCAGCCGATGTTTTCTGCAAGTGAAAGCTTGAGTTTTTCCCAGGGCGTTCGAACATCGGTGGAAATTAACCAGTTTCAGCGGAACATTTATCCACTTAGTAACATGAATTTAAGTCCAATTGATTATACAAGTGCTTTTACAGTTTCTTCTGGCTTCCAAGACATCAGAGATACTGTTAATATTGCAAATAGTTCTCTTTCTTGCATCTCAAGCAGTCACCTATCAACACATACTTCAGGAGCATTTGTAAATCACTCTTCTGTTGGAGGTGCTTATGTGGAACCAAAATCTTTTAATCCTGCTACAAATGGTTCATCTAACTTTGCTTCCAATCCTTCTCCATTGAGTGATCATTTTAACAATGATCAAATTTCTGGCAATAGCTTAAATTTTGCCTCTCCCAGTTCTCACTTTAGGAAAAGTCCAGTTGATTTTTCTTCGACACTTGCTGTTCCTTCGGAGGAAGCGATGCAATGTCAAGATGGCTTACTTGACAATGTTGTAAAGGCTTCATGTTACACGACGCAGCAGCAAAATGTCAGTTCTTCCTTCAACAATACCTTGGATTCTCTTGCTTCTTATAATGAAGACACTAGTTCCATGGTACAAAGTGTACCCCAAACTAATAGTCCATTAACGACTCAGATGCCTCAAGTTGAGAAATTTTATTCCAATGCAAGAGTGATGGAACCACGTGGTGATTGCTTTTTTGAGCAACTGCTTGCTCTAGATGGATTGATTGAGAATAGTTGTGTGTCCTTGGATGACACAATAAGTTAA
Protein sequence

>MS.gene035560.t1

MAVEDRLVSEDVTEEGFPVGMRVLAVDDDQTSLKVLEKHLLTCKYNVTTTTNSVEALELLREKRSMFDLIISDVNMPEMDGFKLLEQVGLEMDLPFIMLSGHDDRERVMKGVMKGACDYLVKPIRLEELKNIWQHVVRKKIESKDQNQGIVSDGVCGQDTSSENIANKKKMHGRKRKEQSEEEEADTDEENDEEHSTRKKPRFVWDNELHKKFVSIVNLLGLDKAYPKKIRDLMNVEGLTRENVASHLQKYRLSLKRPSKQAKVDAALDPLQQKGSVGGYGDFRTLPGSRRILSSTLPTYASSDMFSRLNASSSLNFRRMSSSAPVPPLQSQNIPSFKQPMFSASESLSFSQGVRTSVEINQFQRNIYPLSNMNLSPIDYTSAFTVSSGFQDIRDTVNIANSSLSCISSSHLSTHTSGAFVNHSSVGGAYVEPKSFNPATNGSSNFASNPSPLSDHFNNDQISGNSLNFASPSSHFRKSPVDFSSTLAVPSEEAMQCQDGLLDNVVKASCYTTQQQNVSSSFNNTLDSLASYNEDTSSMVQSVPQTNSPLTTQMPQVEKFYSNARVMEPRGDCFFEQLLALDGLIENSCVSLDDTIS