AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035561

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035561.t1 MTR_4g131580 72.513 593 104 5 1 537 1 590 0.0 822
MS.gene035561.t1 MTR_4g131570 93.484 399 22 2 144 542 202 596 0.0 761
MS.gene035561.t1 MTR_4g131600 65.378 595 145 3 1 542 4 590 0.0 761
MS.gene035561.t1 MTR_2g450070 32.548 679 278 23 3 533 4 650 3.46e-76 254
MS.gene035561.t1 MTR_2g450070 32.548 679 278 23 3 533 4 650 8.86e-76 253
MS.gene035561.t1 MTR_3g102600 50.598 251 92 3 11 261 2 220 4.66e-74 235
MS.gene035561.t1 MTR_2g034960 40.960 354 143 5 6 295 18 369 7.12e-74 246
MS.gene035561.t1 MTR_3g102600 60.215 186 70 1 11 196 2 183 2.12e-69 221
MS.gene035561.t1 MTR_8g079940 50.000 246 67 2 17 206 25 270 9.23e-68 228
MS.gene035561.t1 MTR_1g032570 42.568 296 98 4 16 242 10 302 6.01e-66 225
MS.gene035561.t1 MTR_4g121020 39.323 384 144 12 15 320 29 401 1.38e-64 223
MS.gene035561.t1 MTR_4g121020 39.323 384 144 12 15 320 29 401 1.45e-64 223
MS.gene035561.t1 MTR_3g102590 58.730 189 71 2 17 198 8 196 3.87e-62 204
MS.gene035561.t1 MTR_3g102590 58.378 185 70 2 17 194 8 192 3.57e-59 195
MS.gene035561.t1 MTR_3g086100 46.774 248 74 2 16 205 12 259 4.78e-59 206
MS.gene035561.t1 MTR_3g102600 48.768 203 72 3 59 261 1 171 9.00e-57 188
MS.gene035561.t1 MTR_3g106220 65.693 137 46 1 14 149 5 141 3.77e-54 194
MS.gene035561.t1 MTR_3g106220 45.714 140 64 2 144 279 197 328 1.05e-24 108
MS.gene035561.t1 MTR_1g013170 64.662 133 47 0 15 147 3 135 7.15e-54 190
MS.gene035561.t1 MTR_1g013170 43.448 145 73 2 144 279 190 334 8.30e-23 102
MS.gene035561.t1 MTR_3g102600 60.870 138 50 1 59 196 1 134 2.37e-53 177
MS.gene035561.t1 MTR_1g013180 59.459 148 59 1 2 148 140 287 8.81e-51 185
MS.gene035561.t1 MTR_1g013180 42.105 133 71 4 144 270 341 473 1.07e-18 90.1
MS.gene035561.t1 MTR_7g117705 61.940 134 50 1 15 147 3 136 3.53e-49 176
MS.gene035561.t1 MTR_7g117705 62.069 58 22 0 144 201 190 247 4.97e-17 84.3
MS.gene035561.t1 MTR_1g032570 40.254 236 69 4 76 242 1 233 9.94e-42 158
MS.gene035561.t1 MTR_4g021790 35.874 223 111 3 17 208 10 231 1.95e-39 145
MS.gene035561.t1 MTR_4g021855 34.978 223 113 2 17 208 10 231 4.55e-39 145
MS.gene035561.t1 MTR_4g021760 33.632 223 116 2 17 208 11 232 6.55e-39 144
MS.gene035561.t1 MTR_4g021845 35.885 209 102 3 17 194 10 217 9.59e-36 133
MS.gene035561.t1 MTR_6g045327 32.035 231 124 3 1 201 1 228 5.54e-35 135
MS.gene035561.t1 MTR_0450s0040 34.247 219 105 4 21 201 8 225 4.30e-33 129
MS.gene035561.t1 MTR_3g086100 41.489 188 52 2 76 205 1 188 9.94e-33 131
MS.gene035561.t1 MTR_8g105600 31.535 241 113 3 13 202 5 244 1.11e-30 126
MS.gene035561.t1 MTR_5g055260 36.548 197 106 5 17 202 10 198 1.87e-28 114
MS.gene035561.t1 MTR_7g026400 31.884 207 99 2 47 211 17 223 1.24e-26 114
MS.gene035561.t1 MTR_4g121020 32.673 303 115 12 96 320 6 297 1.25e-26 114
MS.gene035561.t1 MTR_1g013160 69.565 69 21 0 79 147 1 69 1.24e-25 103
MS.gene035561.t1 MTR_3g092780 35.714 126 78 2 21 143 56 181 5.62e-22 100
MS.gene035561.t1 MTR_3g092780 35.714 126 78 2 21 143 56 181 5.97e-22 100
MS.gene035561.t1 MTR_7g118260 36.154 130 80 2 18 144 11 140 1.02e-21 99.4
MS.gene035561.t1 MTR_7g118260 33.540 161 98 4 18 169 11 171 1.82e-21 98.6
MS.gene035561.t1 MTR_8g093040 26.966 267 154 6 1 244 1 249 2.24e-21 94.7
MS.gene035561.t1 MTR_3g092780 35.772 123 76 2 21 140 56 178 2.44e-21 98.2
MS.gene035561.t1 MTR_3g092780 35.772 123 76 2 21 140 56 178 2.45e-21 98.6
MS.gene035561.t1 MTR_6g016850 33.813 139 89 2 13 149 5 142 7.92e-21 90.1
MS.gene035561.t1 MTR_8g024260 37.600 125 75 2 23 144 38 162 1.77e-20 95.5
MS.gene035561.t1 MTR_4g061360 36.691 139 81 4 5 139 75 210 2.63e-19 92.4
MS.gene035561.t1 MTR_4g064730 55.844 77 34 0 126 202 112 188 3.20e-19 89.0
MS.gene035561.t1 MTR_1g067110 36.885 122 74 2 21 139 85 206 4.58e-19 91.3
MS.gene035561.t1 MTR_1g067110 36.885 122 74 2 21 139 85 206 5.83e-19 91.3
MS.gene035561.t1 MTR_3g037390 31.206 141 92 2 3 140 8 146 1.95e-17 85.9
MS.gene035561.t1 MTR_4g108880 32.520 123 80 1 21 140 33 155 2.58e-17 85.5
MS.gene035561.t1 MTR_7g089010 62.712 59 22 0 144 202 90 148 7.77e-17 81.6
MS.gene035561.t1 MTR_4g023980 29.927 137 65 2 63 168 35 171 2.11e-16 77.8
MS.gene035561.t1 MTR_8g032710 29.412 136 91 2 20 150 20 155 1.10e-15 80.1
MS.gene035561.t1 MTR_5g014040 38.095 126 74 2 15 139 12 134 1.59e-15 79.7
MS.gene035561.t1 MTR_5g014040 60.377 53 21 0 150 202 316 368 1.09e-13 73.9
MS.gene035561.t1 MTR_5g014040 38.095 126 74 2 15 139 12 134 1.78e-15 79.7
MS.gene035561.t1 MTR_5g014040 55.172 58 21 1 150 202 316 373 9.51e-12 67.8
MS.gene035561.t1 MTR_4g098870 36.508 126 76 2 15 139 12 134 4.75e-15 78.6
MS.gene035561.t1 MTR_4g098870 67.347 49 16 0 150 198 315 363 4.47e-14 75.5
MS.gene035561.t1 MTR_4g098870 36.508 126 76 2 15 139 32 154 6.17e-15 78.2
MS.gene035561.t1 MTR_4g098870 67.347 49 16 0 150 198 335 383 4.68e-14 75.5
MS.gene035561.t1 MTR_4g098870 36.508 126 76 2 15 139 51 173 6.33e-15 78.2
MS.gene035561.t1 MTR_4g098870 67.347 49 16 0 150 198 354 402 5.28e-14 75.1
MS.gene035561.t1 MTR_4g098870 36.508 126 76 2 15 139 12 134 8.97e-15 77.4
MS.gene035561.t1 MTR_4g098870 67.347 49 16 0 150 198 315 363 5.53e-14 75.1
MS.gene035561.t1 MTR_4g098870 36.508 126 76 2 15 139 32 154 1.23e-14 77.0
MS.gene035561.t1 MTR_4g098870 67.347 49 16 0 150 198 335 383 6.31e-14 74.7
MS.gene035561.t1 MTR_1g093080 49.254 67 33 1 139 204 205 271 6.25e-13 70.9
MS.gene035561.t1 MTR_4g078810 56.250 48 21 0 150 197 163 210 3.09e-12 68.9
MS.gene035561.t1 MTR_2g090675 56.250 48 21 0 150 197 166 213 6.17e-12 68.2
MS.gene035561.t1 MTR_5g014040 55.172 58 21 1 150 202 276 333 7.94e-12 68.2
MS.gene035561.t1 MTR_6g088950 28.467 137 92 2 19 154 5 136 1.24e-11 64.7
MS.gene035561.t1 MTR_1874s0010 31.858 113 73 2 19 129 2 112 3.69e-11 63.9
MS.gene035561.t1 MTR_4g086835 46.667 60 32 0 143 202 272 331 5.95e-11 65.1
MS.gene035561.t1 MTR_7g490310 28.105 153 87 3 23 154 11 161 8.80e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035561.t1 AT4G31920 47.352 321 112 8 1 268 1 317 9.59e-79 258
MS.gene035561.t1 AT5G58080 43.836 365 137 7 9 314 6 361 3.85e-75 250
MS.gene035561.t1 AT1G67710 49.393 247 66 7 18 205 8 254 4.23e-57 199
MS.gene035561.t1 AT2G25180 66.197 142 44 1 1 142 1 138 4.33e-56 198
MS.gene035561.t1 AT2G25180 40.659 182 96 6 149 319 199 379 4.37e-23 103
MS.gene035561.t1 AT3G16857 62.698 126 47 0 15 140 31 156 1.88e-46 173
MS.gene035561.t1 AT3G16857 40.152 132 75 2 144 271 236 367 8.59e-20 93.6
MS.gene035561.t1 AT3G16857 62.698 126 47 0 15 140 31 156 2.70e-46 172
MS.gene035561.t1 AT3G16857 40.152 132 75 2 144 271 236 367 8.42e-20 93.6
MS.gene035561.t1 AT2G01760 57.364 129 54 1 13 140 3 131 1.32e-42 157
MS.gene035561.t1 AT2G01760 61.538 65 25 0 143 207 198 262 9.20e-18 85.9
MS.gene035561.t1 AT5G49240 35.685 241 98 2 11 194 32 272 1.43e-40 149
MS.gene035561.t1 AT2G01760 47.742 155 52 2 15 140 5 159 9.53e-37 141
MS.gene035561.t1 AT2G01760 61.538 65 25 0 143 207 226 290 1.03e-17 85.9
MS.gene035561.t1 AT3G16857 68.235 85 27 0 56 140 2 86 3.29e-32 131
MS.gene035561.t1 AT3G16857 40.152 132 75 2 144 271 166 297 6.80e-20 94.0
MS.gene035561.t1 AT1G49190 28.990 307 139 9 17 260 30 320 6.95e-32 127
MS.gene035561.t1 AT1G67710 44.444 189 46 7 76 205 1 189 3.46e-31 126
MS.gene035561.t1 AT3G62670 32.862 283 125 9 22 249 42 314 3.62e-31 125
MS.gene035561.t1 AT1G49190 37.600 125 78 0 17 141 30 154 5.99e-23 103
MS.gene035561.t1 AT2G46790 37.984 129 77 2 21 146 37 165 2.60e-22 100
MS.gene035561.t1 AT2G46790 37.984 129 77 2 21 146 37 165 3.30e-22 100
MS.gene035561.t1 AT5G24470 36.000 125 77 2 19 140 48 172 6.72e-22 99.8
MS.gene035561.t1 AT2G01760 65.152 66 22 1 76 140 1 66 1.80e-20 92.8
MS.gene035561.t1 AT2G01760 61.538 65 25 0 143 207 133 197 4.47e-18 85.9
MS.gene035561.t1 AT5G60100 37.795 127 75 3 17 139 59 185 1.28e-19 91.7
MS.gene035561.t1 AT5G60100 37.795 127 75 3 17 139 59 185 1.28e-19 91.7
MS.gene035561.t1 AT5G60100 37.795 127 75 3 17 139 59 185 1.28e-19 91.7
MS.gene035561.t1 AT5G60100 37.795 127 75 3 17 139 59 185 2.42e-19 91.7
MS.gene035561.t1 AT5G60100 37.795 127 75 3 17 139 59 185 2.42e-19 91.7
MS.gene035561.t1 AT5G60100 37.795 127 75 3 17 139 59 185 2.42e-19 91.7
MS.gene035561.t1 AT5G60100 37.795 127 75 3 17 139 59 185 3.55e-19 91.3
MS.gene035561.t1 AT5G59570 66.667 60 20 0 143 202 139 198 5.26e-19 88.2
MS.gene035561.t1 AT5G59570 66.667 60 20 0 143 202 139 198 5.26e-19 88.2
MS.gene035561.t1 AT1G67710 39.286 168 43 7 97 205 13 180 5.56e-19 90.1
MS.gene035561.t1 AT3G46640 66.102 59 20 0 144 202 143 201 1.42e-18 87.4
MS.gene035561.t1 AT3G46640 66.102 59 20 0 144 202 143 201 1.42e-18 87.4
MS.gene035561.t1 AT3G46640 66.102 59 20 0 144 202 143 201 1.75e-18 87.0
MS.gene035561.t1 AT5G02810 37.705 122 73 2 21 139 78 199 7.78e-18 87.4
MS.gene035561.t1 AT5G61380 34.146 123 78 1 21 140 19 141 3.04e-17 85.5
MS.gene035561.t1 AT5G05090 62.712 59 22 0 144 202 80 138 3.09e-17 82.4
MS.gene035561.t1 AT2G40970 62.712 59 22 0 144 202 104 162 3.21e-17 82.0
MS.gene035561.t1 AT3G10760 62.712 59 22 0 144 202 104 162 5.00e-17 82.8
MS.gene035561.t1 AT5G44190 57.627 59 25 0 144 202 146 204 5.18e-14 73.9
MS.gene035561.t1 AT5G44190 57.627 59 25 0 144 202 146 204 5.74e-14 74.3
MS.gene035561.t1 AT5G07210 31.200 125 83 2 21 144 16 138 2.43e-13 73.2
MS.gene035561.t1 AT5G07210 48.649 74 31 1 134 207 218 284 2.85e-13 72.8
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 2.47e-13 72.8
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.21e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 2.47e-13 72.8
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.21e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 2.47e-13 72.8
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.21e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 2.47e-13 72.8
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.21e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 2.47e-13 72.8
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.21e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 2.47e-13 72.8
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 7.21e-12 68.2
MS.gene035561.t1 AT5G07210 48.649 74 31 1 134 207 226 292 2.88e-13 72.8
MS.gene035561.t1 AT5G07210 29.323 133 83 3 21 144 16 146 1.52e-11 67.4
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 3.45e-13 72.4
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.24e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 3.45e-13 72.4
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.24e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 3.45e-13 72.4
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.24e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 3.45e-13 72.4
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.24e-12 68.2
MS.gene035561.t1 AT4G18020 37.879 132 73 3 15 139 12 141 3.45e-13 72.4
MS.gene035561.t1 AT4G18020 53.125 64 23 1 138 201 296 352 8.24e-12 68.2
MS.gene035561.t1 AT2G20570 59.615 52 21 0 150 201 158 209 1.63e-12 70.1
MS.gene035561.t1 AT2G20570 59.615 52 21 0 150 201 174 225 1.90e-12 69.7
MS.gene035561.t1 AT2G03500 43.662 71 39 1 148 217 236 306 5.82e-12 68.2
MS.gene035561.t1 AT2G27070 52.542 59 28 0 150 208 230 288 1.52e-11 67.4

Find 109 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCGCTCAGTTCTCAATTT+AGG 0.173828 8.4:+2043129 MS.gene035561:CDS
AGCTTGAATCCATCCATTTC+TGG 0.203555 8.4:-2041418 None:intergenic
TTATAGTTTGTACCTGTAAA+TGG 0.224166 8.4:-2042212 None:intergenic
TGAAAACTCGAGTGCTTTAA+AGG 0.227005 8.4:+2042755 MS.gene035561:CDS
AAACAATGGCTGCTTGTAAC+TGG 0.250042 8.4:-2042724 None:intergenic
CGTCATTGATGTTCCTTTAG+AGG 0.255703 8.4:+2043181 MS.gene035561:CDS
TATTAGCTTGGGGTACATTA+TGG 0.266193 8.4:-2043343 None:intergenic
TCCTGTGGGAATGCGTGTTC+TGG 0.289810 8.4:+2040173 MS.gene035561:CDS
CTTCTCATGTGCAAGTATAA+TGG 0.318350 8.4:+2040240 MS.gene035561:CDS
GAGAAAGAAGATTGAAAGTA+AGG 0.340472 8.4:+2041712 MS.gene035561:CDS
TGAGGCATCTGAGTGATTAA+TGG 0.341513 8.4:-2043368 None:intergenic
CAATGTCAAGATGGATTACT+TGG 0.343348 8.4:+2043212 MS.gene035561:CDS
AAGAATTCAGTAGCTGGATA+TGG 0.366836 8.4:+2042540 MS.gene035561:CDS
AGTGCATTTACAGATTCTTC+TGG 0.369412 8.4:+2042864 MS.gene035561:CDS
TTATTCAGATGAAAGAACTA+TGG 0.373116 8.4:+2043403 MS.gene035561:CDS
CATTGCATCGTTTCCTCTAA+AGG 0.374710 8.4:-2043194 None:intergenic
AGGACTGGAAGCAAAGTTAT+AGG 0.378746 8.4:-2043048 None:intergenic
GATGGCTTTGGAGAATCCTT+TGG 0.383986 8.4:+2040122 None:intergenic
TCTCAAAAGGCCATCTAAAC+AGG 0.387755 8.4:+2042482 MS.gene035561:CDS
GATAAATGTTCCGGTGAAAC+TGG 0.391840 8.4:-2042803 None:intergenic
TCAGTAATGATACCTTTATT+CGG 0.392934 8.4:-2041737 None:intergenic
AAATGTGGCTAGCCATTTAC+AGG 0.398143 8.4:+2042200 MS.gene035561:CDS
CTCTTGAATTATGTGTTTGT+AGG 0.401274 8.4:-2042953 None:intergenic
TCTACTCTAGCCTGTTTAGA+TGG 0.401513 8.4:-2042492 None:intergenic
ATTCTGGTTCATGATGGCTT+TGG 0.405441 8.4:+2040110 None:intergenic
GAAAGTAAGGATCCGAATAA+AGG 0.416092 8.4:+2041725 MS.gene035561:CDS
TTCAGAATAGTTGTTTGCCT+TGG 0.419816 8.4:+2043480 MS.gene035561:CDS
CTTAAGATTCTTCTTGATCC+AGG 0.420041 8.4:-2042579 None:intergenic
TGTCGGCTGAACGCACCATC+TGG 0.422911 8.4:+2042639 MS.gene035561:CDS
TGAGCAAGTTGGACATGAAA+TGG 0.425491 8.4:+2041444 MS.gene035561:CDS
GAGCAATTGCTTGATGTAGA+TGG 0.429100 8.4:+2043452 MS.gene035561:CDS
TTTATAAATCACCCATCTGT+TGG 0.434200 8.4:+2042978 MS.gene035561:CDS
AATGCACTTGAATCATCAAT+TGG 0.435178 8.4:-2042849 None:intergenic
GATTAATGGACTATTAGCTT+GGG 0.435758 8.4:-2043354 None:intergenic
TACCAGGTGGATAAATGTTC+CGG 0.439267 8.4:-2042812 None:intergenic
CATCTAGTAACATGTATTGT+CGG 0.449068 8.4:+2042622 MS.gene035561:CDS
TCTCTTGCTTCTTCTAATGC+AGG 0.449172 8.4:+2043305 MS.gene035561:CDS
TAACATTAGCTCTGATGTCT+TGG 0.449900 8.4:-2042890 None:intergenic
CACCGGAACATTTATCCACC+TGG 0.455890 8.4:+2042810 MS.gene035561:CDS
TTGGAGGTTGAAGATGTCTT+CGG 0.457510 8.4:+2040141 MS.gene035561:CDS
TTATAGGAACCACTTGTAGC+AGG 0.464041 8.4:-2043032 None:intergenic
AATACAAACTTAGTCTCAAA+AGG 0.473606 8.4:+2042469 MS.gene035561:CDS
TGGAAGAGCTTAAGAACATA+TGG 0.475204 8.4:+2041678 MS.gene035561:CDS
GGAGATTTGTGTACCTTACC+TGG 0.477382 8.4:+2042561 MS.gene035561:CDS
TGATTAATGGACTATTAGCT+TGG 0.477988 8.4:-2043355 None:intergenic
TGATGTAAACATGCCAGAAA+TGG 0.478571 8.4:+2041405 MS.gene035561:CDS
TTCTTCCTTCAACAATACCT+TGG 0.480791 8.4:+2043280 MS.gene035561:CDS
TTAAGCTCTTCCAATCGGAT+TGG 0.487989 8.4:-2041668 None:intergenic
TGTTCTTAAGCTCTTCCAAT+CGG 0.493421 8.4:-2041673 None:intergenic
GGCTTTGGAGAATCCTTTGG+AGG 0.494937 8.4:+2040125 MS.gene035561:CDS
CTCACTCAATGGAGAAGGAC+TGG 0.495705 8.4:-2043063 None:intergenic
TGAATGTTGAAGGGCTTACA+AGG 0.497287 8.4:+2042175 MS.gene035561:CDS
GAAAGAACTATGGAATCAAA+TGG 0.497837 8.4:+2043413 MS.gene035561:CDS
CAGTCCTTCTCCATTGAGTG+AGG 0.499289 8.4:+2043064 MS.gene035561:CDS
CTACAAAAGAATTCAGTAGC+TGG 0.500934 8.4:+2042534 MS.gene035561:CDS
ATCTGTTGGAGGTCCTGCTA+TGG 0.503540 8.4:+2042992 MS.gene035561:CDS
GAAAACTCGAGTGCTTTAAA+GGG 0.504861 8.4:+2042756 MS.gene035561:CDS
AGAATCCAAGGTATTGTTGA+AGG 0.506294 8.4:-2043285 None:intergenic
TTATTAAAATCCTCACTCAA+TGG 0.507785 8.4:-2043074 None:intergenic
CAGGAAATGATGATAGAGAG+AGG 0.513398 8.4:+2041600 MS.gene035561:CDS
TGTGATCATATAGTGTTGTC+AGG 0.513792 8.4:+2041581 MS.gene035561:intron
AGAAGAAGCAAGAGAATCCA+AGG 0.514940 8.4:-2043297 None:intergenic
GGACTTAAGTTCATGTTACC+AGG 0.516544 8.4:-2042828 None:intergenic
TTCAAGCTCCTTGAGCAAGT+TGG 0.519096 8.4:+2041433 MS.gene035561:CDS
GATTCTTCTTGATCCAGGTA+AGG 0.519260 8.4:-2042574 None:intergenic
TCAAACTTGTCTCAAACTGT+TGG 0.520173 8.4:+2040209 MS.gene035561:CDS
AACAATGGCTGCTTGTAACT+GGG 0.528436 8.4:-2042723 None:intergenic
GAATAAAATTTCTCAACTTG+AGG 0.529905 8.4:-2043386 None:intergenic
GTCACATAAACTCACTATCA+AGG 0.536123 8.4:-2041985 None:intergenic
TCTTGTGAAACCAATCCGAT+TGG 0.545613 8.4:+2041658 MS.gene035561:CDS
TGTGACTTGATGAATGTTGA+AGG 0.547972 8.4:+2042165 MS.gene035561:CDS
TTCACTTATTGTGTCATCCA+AGG 0.551820 8.4:-2043497 None:intergenic
ATAAATCACCCATCTGTTGG+AGG 0.552382 8.4:+2042981 MS.gene035561:CDS
TTGAAGCGCTAGAATTGTTG+AGG 0.553092 8.4:+2041350 MS.gene035561:CDS
GTGTGTGAGCTTACTGATGA+AGG 0.556710 8.4:-2041473 None:intergenic
TAATGCAGGCACTAGTTCCA+TGG 0.559067 8.4:+2043319 MS.gene035561:CDS
TGAAGCGCTAGAATTGTTGA+GGG 0.561694 8.4:+2041351 MS.gene035561:CDS
AGGAAATGATGATAGAGAGA+GGG 0.563967 8.4:+2041601 MS.gene035561:CDS
TTTCATGTCCAACTTGCTCA+AGG 0.564395 8.4:-2041441 None:intergenic
TGGAAATTAACCAGTTTCAC+CGG 0.564960 8.4:+2042793 MS.gene035561:CDS
ATGTTACTAGATGCATATGT+TGG 0.572114 8.4:-2042612 None:intergenic
AAATCCTCACTCAATGGAGA+AGG 0.576468 8.4:-2043068 None:intergenic
ATAGCAGGACCTCCAACAGA+TGG 0.581262 8.4:-2042990 None:intergenic
GAAACGATGCAATGTCAAGA+TGG 0.581317 8.4:+2043203 MS.gene035561:CDS
CCATCTGGATTGAACTTGAG+AGG 0.585944 8.4:+2042654 MS.gene035561:CDS
GCCAGAACACGCATTCCCAC+AGG 0.587093 8.4:-2040174 None:intergenic
TATAGGAACCACTTGTAGCA+GGG 0.588786 8.4:-2043031 None:intergenic
AAAGGGTGTTCGAACGTCAG+TGG 0.592037 8.4:+2042773 MS.gene035561:CDS
ATTACTTGGGAATGTTGTAA+CGG 0.596089 8.4:+2043226 MS.gene035561:CDS
GATAGAGAGAGGGTGATGAA+AGG 0.596610 8.4:+2041611 MS.gene035561:CDS
CCTCTCAAGTTCAATCCAGA+TGG 0.601365 8.4:-2042654 None:intergenic
GTAAACATGCCAGAAATGGA+TGG 0.605525 8.4:+2041409 MS.gene035561:CDS
TAGCAGGACCTCCAACAGAT+GGG 0.607030 8.4:-2042989 None:intergenic
AAGACATCTTCAACCTCCAA+AGG 0.613959 8.4:-2040138 None:intergenic
CCTAAATTGAGAACTGAGCG+AGG 0.617995 8.4:-2043129 None:intergenic
GTGATGAAAGGTGTGATGAA+AGG 0.623017 8.4:+2041623 MS.gene035561:CDS
AGGGCTTACAAGGGAAAATG+TGG 0.634196 8.4:+2042185 MS.gene035561:CDS
ATTAATGGACTATTAGCTTG+GGG 0.634710 8.4:-2043353 None:intergenic
GTATCTATAGTTAGTCAACT+CGG 0.636343 8.4:+2041962 MS.gene035561:CDS
TGAGCTTACTGATGAAGGGT+AGG 0.644624 8.4:-2041468 None:intergenic
GTGACTTGATGAATGTTGAA+GGG 0.645884 8.4:+2042166 MS.gene035561:CDS
AATGTCAAGATGGATTACTT+GGG 0.659008 8.4:+2043213 MS.gene035561:CDS
TGTGTGAGCTTACTGATGAA+GGG 0.665662 8.4:-2041472 None:intergenic
CTTAAGTTCATGTTACCAGG+TGG 0.668465 8.4:-2042825 None:intergenic
GAATGTTGAAGGGCTTACAA+GGG 0.675399 8.4:+2042176 MS.gene035561:CDS
TTACACGCAGCAGCAAAACG+TGG 0.681302 8.4:+2043256 MS.gene035561:CDS
TTGGGGTACATTATGGACCA+TGG 0.694419 8.4:-2043336 None:intergenic
TACACGCAGCAGCAAAACGT+GGG 0.705438 8.4:+2043257 MS.gene035561:CDS
AGAACTATGGAATCAAATGG+TGG 0.707711 8.4:+2043416 MS.gene035561:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTATATCTAAAATCA+TGG - chr8.4:2041094-2041113 None:intergenic 10.0%
!!! AGATTTTAAAATTCAACTTT+AGG - chr8.4:2041151-2041170 None:intergenic 15.0%
!!! TATGAAATATTTTGACAAAA+GGG - chr8.4:2042242-2042261 None:intergenic 15.0%
!!! TTATGTTTCTACTAATTTTT+TGG - chr8.4:2040695-2040714 None:intergenic 15.0%
!! AAAATGAGAAAACAGTATTT+AGG - chr8.4:2040963-2040982 None:intergenic 20.0%
!! AAAGTCGAAAATAAAAATAG+AGG + chr8.4:2040825-2040844 MS.gene035561:intron 20.0%
!! ATTCACTTCTTATATTCATA+TGG + chr8.4:2041532-2041551 MS.gene035561:intron 20.0%
!! TAGGTCAACATATATTATTA+GGG - chr8.4:2040653-2040672 None:intergenic 20.0%
!!! AAAGATCACTACTATTTTAT+TGG + chr8.4:2041174-2041193 MS.gene035561:intron 20.0%
!!! AAATATTTTCGATGTAATCT+TGG + chr8.4:2040571-2040590 MS.gene035561:intron 20.0%
!!! AAGATCACTACTATTTTATT+GGG + chr8.4:2041175-2041194 MS.gene035561:intron 20.0%
!!! AATAATTCAGTTGTTTTGAT+TGG + chr8.4:2040862-2040881 MS.gene035561:intron 20.0%
!!! ATGAAATATTTTGACAAAAG+GGG - chr8.4:2042241-2042260 None:intergenic 20.0%
!!! GAGAGACATTTAAAATAAAA+AGG - chr8.4:2041275-2041294 None:intergenic 20.0%
!!! GTATGAAATATTTTGACAAA+AGG - chr8.4:2042243-2042262 None:intergenic 20.0%
!!! TAGTAAACCCTTTTTTTAAT+CGG + chr8.4:2040389-2040408 MS.gene035561:intron 20.0%
!!! TGTTACTTTACTAATTTGAT+TGG + chr8.4:2042024-2042043 MS.gene035561:intron 20.0%
! AAAAATAAAGGAACAAAGTG+AGG + chr8.4:2041823-2041842 MS.gene035561:intron 25.0%
! AATACAAACTTAGTCTCAAA+AGG + chr8.4:2042469-2042488 MS.gene035561:CDS 25.0%
! ACCCGAAATAAACAAAAAAT+TGG - chr8.4:2040602-2040621 None:intergenic 25.0%
! ACTGAAAGAAAGAAGAAAAA+AGG - chr8.4:2040274-2040293 None:intergenic 25.0%
! CAGTGGTATAAACTTATAAT+TGG + chr8.4:2040352-2040371 MS.gene035561:intron 25.0%
! CCCGAAATAAACAAAAAATT+GGG - chr8.4:2040601-2040620 None:intergenic 25.0%
! CCGAAATAAACAAAAAATTG+GGG - chr8.4:2040600-2040619 None:intergenic 25.0%
! CTGAAAGAAAGAAGAAAAAA+GGG - chr8.4:2040273-2040292 None:intergenic 25.0%
! GAATAAAATTTCTCAACTTG+AGG - chr8.4:2043389-2043408 None:intergenic 25.0%
! GTAGGTCAACATATATTATT+AGG - chr8.4:2040654-2040673 None:intergenic 25.0%
! GTTGAAATTTCAACGAATAA+TGG - chr8.4:2042384-2042403 None:intergenic 25.0%
! TAATACACATGCTAAAACAA+AGG - chr8.4:2041498-2041517 None:intergenic 25.0%
! TCAGTAATGATACCTTTATT+CGG - chr8.4:2041740-2041759 None:intergenic 25.0%
! TGATGATTGAAACTTACATA+TGG + chr8.4:2042078-2042097 MS.gene035561:intron 25.0%
! TGTAAGTTTCAATCATCAAA+TGG - chr8.4:2042076-2042095 None:intergenic 25.0%
! TTATTAAAATCCTCACTCAA+TGG - chr8.4:2043077-2043096 None:intergenic 25.0%
! TTATTCAGATGAAAGAACTA+TGG + chr8.4:2043403-2043422 MS.gene035561:CDS 25.0%
! TTTGGCAAATATACTTCTTT+GGG - chr8.4:2040677-2040696 None:intergenic 25.0%
!! ATCAAGTCACATATTTTCTT+AGG - chr8.4:2042156-2042175 None:intergenic 25.0%
!! CTCTTTTATGAAACATAAGT+GGG + chr8.4:2042407-2042426 MS.gene035561:intron 25.0%
!! TAACTTATAAGCTTTGTAGT+CGG + chr8.4:2040487-2040506 MS.gene035561:intron 25.0%
!! TCTCTTTTATGAAACATAAG+TGG + chr8.4:2042406-2042425 MS.gene035561:intron 25.0%
!! TTATAGTTTGTACCTGTAAA+TGG - chr8.4:2042215-2042234 None:intergenic 25.0%
!! TTTTCACTTGCAAAAAACAA+TGG - chr8.4:2042741-2042760 None:intergenic 25.0%
!! TTTTGGCAAATATACTTCTT+TGG - chr8.4:2040678-2040697 None:intergenic 25.0%
!!! AATAAAGGTATCATTACTGA+TGG + chr8.4:2041740-2041759 MS.gene035561:intron 25.0%
!!! AGTAAACCCTTTTTTTAATC+GGG + chr8.4:2040390-2040409 MS.gene035561:intron 25.0%
!!! ATAAAGGTATCATTACTGAT+GGG + chr8.4:2041741-2041760 MS.gene035561:intron 25.0%
!!! ATTTGATTGGCTTTAAAACA+TGG + chr8.4:2042037-2042056 MS.gene035561:intron 25.0%
!!! CCCAATTTTTTGTTTATTTC+GGG + chr8.4:2040598-2040617 MS.gene035561:intron 25.0%
!!! CCCCAATTTTTTGTTTATTT+CGG + chr8.4:2040597-2040616 MS.gene035561:intron 25.0%
!!! TCTATATTTGTTGTAAAGAC+CGG - chr8.4:2040772-2040791 None:intergenic 25.0%
AAATATCGTCGAAGTTAAAG+GGG + chr8.4:2042301-2042320 MS.gene035561:intron 30.0%
AACTTTGCTAGCAATGAAAA+TGG + chr8.4:2040795-2040814 MS.gene035561:intron 30.0%
AATGCACTTGAATCATCAAT+TGG - chr8.4:2042852-2042871 None:intergenic 30.0%
ATGTTACTAGATGCATATGT+TGG - chr8.4:2042615-2042634 None:intergenic 30.0%
ATTACTTGGGAATGTTGTAA+CGG + chr8.4:2043226-2043245 MS.gene035561:CDS 30.0%
ATTTGCCCCGATTAAAAAAA+GGG - chr8.4:2040399-2040418 None:intergenic 30.0%
CAAATATCGTCGAAGTTAAA+GGG + chr8.4:2042300-2042319 MS.gene035561:intron 30.0%
CATATGCTTGTATGCAAATA+TGG + chr8.4:2042340-2042359 MS.gene035561:intron 30.0%
CATCTAGTAACATGTATTGT+CGG + chr8.4:2042622-2042641 MS.gene035561:CDS 30.0%
CTCTTGAATTATGTGTTTGT+AGG - chr8.4:2042956-2042975 None:intergenic 30.0%
GAAAGAACTATGGAATCAAA+TGG + chr8.4:2043413-2043432 MS.gene035561:CDS 30.0%
GAGAAAGAAGATTGAAAGTA+AGG + chr8.4:2041712-2041731 MS.gene035561:CDS 30.0%
GTATCTATAGTTAGTCAACT+CGG + chr8.4:2041962-2041981 MS.gene035561:CDS 30.0%
GTCAACATATATTATTAGGG+TGG - chr8.4:2040650-2040669 None:intergenic 30.0%
TAGGAATAATAGACCTCATT+GGG + chr8.4:2041219-2041238 MS.gene035561:intron 30.0%
TGAATTACTACTTTGATGCT+TGG + chr8.4:2041297-2041316 MS.gene035561:intron 30.0%
TGATTGAATGTAACATGTGT+CGG - chr8.4:2040539-2040558 None:intergenic 30.0%
TTGGCAAATATACTTCTTTG+GGG - chr8.4:2040676-2040695 None:intergenic 30.0%
TTTATAAATCACCCATCTGT+TGG + chr8.4:2042978-2042997 MS.gene035561:CDS 30.0%
! AAAGATTTTTGTTCCATAGC+AGG - chr8.4:2043008-2043027 None:intergenic 30.0%
! AAATTCAACTTTAGGAGAAC+AGG - chr8.4:2041143-2041162 None:intergenic 30.0%
! AGTTGAAGAAAGTACATGTT+AGG - chr8.4:2042115-2042134 None:intergenic 30.0%
! GTTAGCAATATTTTCAGATG+AGG - chr8.4:2041783-2041802 None:intergenic 30.0%
!! AATGTCAAGATGGATTACTT+GGG + chr8.4:2043213-2043232 MS.gene035561:CDS 30.0%
!! ATCATCAAATGGTCTTGTTA+AGG - chr8.4:2042065-2042084 None:intergenic 30.0%
!! ATTAATGGACTATTAGCTTG+GGG - chr8.4:2043356-2043375 None:intergenic 30.0%
!! GATTAATGGACTATTAGCTT+GGG - chr8.4:2043357-2043376 None:intergenic 30.0%
!! TGATTAATGGACTATTAGCT+TGG - chr8.4:2043358-2043377 None:intergenic 30.0%
!!! GTAAACCCTTTTTTTAATCG+GGG + chr8.4:2040391-2040410 MS.gene035561:intron 30.0%
!!! GTTCCTTTATTTTTTGTCCA+CGG - chr8.4:2041817-2041836 None:intergenic 30.0%
!!! TTCCTTTATTTTTTGTCCAC+GGG - chr8.4:2041816-2041835 None:intergenic 30.0%
AACCCGTGGACAAAAAATAA+AGG + chr8.4:2041811-2041830 MS.gene035561:intron 35.0%
AAGAATTCAGTAGCTGGATA+TGG + chr8.4:2042540-2042559 MS.gene035561:CDS 35.0%
AGAACTATGGAATCAAATGG+TGG + chr8.4:2043416-2043435 MS.gene035561:CDS 35.0%
AGGAAATGATGATAGAGAGA+GGG + chr8.4:2041601-2041620 MS.gene035561:CDS 35.0%
AGTGCATTTACAGATTCTTC+TGG + chr8.4:2042864-2042883 MS.gene035561:CDS 35.0%
CATTTGCCCCGATTAAAAAA+AGG - chr8.4:2040400-2040419 None:intergenic 35.0%
CGACGATATTTGCAAAAATG+TGG - chr8.4:2042292-2042311 None:intergenic 35.0%
CTACAAAAGAATTCAGTAGC+TGG + chr8.4:2042534-2042553 MS.gene035561:CDS 35.0%
CTTAAGATTCTTCTTGATCC+AGG - chr8.4:2042582-2042601 None:intergenic 35.0%
CTTCTCATGTGCAAGTATAA+TGG + chr8.4:2040240-2040259 MS.gene035561:CDS 35.0%
GAAAACGCAGAAGAAGATAA+TGG + chr8.4:2041863-2041882 MS.gene035561:intron 35.0%
GAAAGTAAGGATCCGAATAA+AGG + chr8.4:2041725-2041744 MS.gene035561:CDS 35.0%
GCAAATATCGTCGAAGTTAA+AGG + chr8.4:2042299-2042318 MS.gene035561:intron 35.0%
GTAGGAATAATAGACCTCAT+TGG + chr8.4:2041218-2041237 MS.gene035561:intron 35.0%
GTCACATAAACTCACTATCA+AGG - chr8.4:2041988-2042007 None:intergenic 35.0%
GTGACTTGATGAATGTTGAA+GGG + chr8.4:2042166-2042185 MS.gene035561:CDS 35.0%
GTGTCGAAGAAAAATCAGTT+GGG - chr8.4:2043160-2043179 None:intergenic 35.0%
TAATGGTAGAGAGAATGATG+AGG + chr8.4:2041880-2041899 MS.gene035561:intron 35.0%
TCAAACTTGTCTCAAACTGT+TGG + chr8.4:2040209-2040228 MS.gene035561:CDS 35.0%
TGAAAACTCGAGTGCTTTAA+AGG + chr8.4:2042755-2042774 MS.gene035561:CDS 35.0%
TGATGTAAACATGCCAGAAA+TGG + chr8.4:2041405-2041424 MS.gene035561:CDS 35.0%
TGCTAGCAATGAAAATGGTA+AGG + chr8.4:2040800-2040819 MS.gene035561:intron 35.0%
TGGAAATTAACCAGTTTCAC+CGG + chr8.4:2042793-2042812 MS.gene035561:CDS 35.0%
TGGAAGAGCTTAAGAACATA+TGG + chr8.4:2041678-2041697 MS.gene035561:CDS 35.0%
TGTGACTTGATGAATGTTGA+AGG + chr8.4:2042165-2042184 MS.gene035561:CDS 35.0%
TGTGATCATATAGTGTTGTC+AGG + chr8.4:2041581-2041600 MS.gene035561:intron 35.0%
TGTTCTTAAGCTCTTCCAAT+CGG - chr8.4:2041676-2041695 None:intergenic 35.0%
TTCAGAATAGTTGTTTGCCT+TGG + chr8.4:2043480-2043499 MS.gene035561:CDS 35.0%
TTCTTCAACTGTTATTGCAG+AGG + chr8.4:2042125-2042144 MS.gene035561:intron 35.0%
TTCTTCCTTCAACAATACCT+TGG + chr8.4:2043280-2043299 MS.gene035561:CDS 35.0%
! ACTGAATTCTTTTGTAGGTG+AGG - chr8.4:2042531-2042550 None:intergenic 35.0%
! CAGCTACTGAATTCTTTTGT+AGG - chr8.4:2042536-2042555 None:intergenic 35.0%
! GAAAACTCGAGTGCTTTAAA+GGG + chr8.4:2042756-2042775 MS.gene035561:CDS 35.0%
! TATTAGCTTGGGGTACATTA+TGG - chr8.4:2043346-2043365 None:intergenic 35.0%
!! AGAATCCAAGGTATTGTTGA+AGG - chr8.4:2043288-2043307 None:intergenic 35.0%
!! CAAATATACTTCTTTGGGGT+AGG - chr8.4:2040672-2040691 None:intergenic 35.0%
!! CAATGTCAAGATGGATTACT+TGG + chr8.4:2043212-2043231 MS.gene035561:CDS 35.0%
!! TAACATTAGCTCTGATGTCT+TGG - chr8.4:2042893-2042912 None:intergenic 35.0%
!!! TCGTCTTGTTTTGACATTTC+CGG + chr8.4:2040750-2040769 MS.gene035561:intron 35.0%
AAACAATGGCTGCTTGTAAC+TGG - chr8.4:2042727-2042746 None:intergenic 40.0%
AAAGGCTTCCTGCGTTATAA+AGG - chr8.4:2041257-2041276 None:intergenic 40.0%
AAATCCTCACTCAATGGAGA+AGG - chr8.4:2043071-2043090 None:intergenic 40.0%
AACAATGGCTGCTTGTAACT+GGG - chr8.4:2042726-2042745 None:intergenic 40.0%
AAGACATCTTCAACCTCCAA+AGG - chr8.4:2040141-2040160 None:intergenic 40.0%
AAGTTATAACCAAGACGTCG+TGG - chr8.4:2040473-2040492 None:intergenic 40.0%
AGAAGAAGCAAGAGAATCCA+AGG - chr8.4:2043300-2043319 None:intergenic 40.0%
AGCTTGAATCCATCCATTTC+TGG - chr8.4:2041421-2041440 None:intergenic 40.0%
AGGACTGGAAGCAAAGTTAT+AGG - chr8.4:2043051-2043070 None:intergenic 40.0%
AGTTTATACCACTGCATGAG+AGG - chr8.4:2040346-2040365 None:intergenic 40.0%
ATAAATCACCCATCTGTTGG+AGG + chr8.4:2042981-2043000 MS.gene035561:CDS 40.0%
CAGGAAATGATGATAGAGAG+AGG + chr8.4:2041600-2041619 MS.gene035561:CDS 40.0%
CATTGCATCGTTTCCTCTAA+AGG - chr8.4:2043197-2043216 None:intergenic 40.0%
CGTCATTGATGTTCCTTTAG+AGG + chr8.4:2043181-2043200 MS.gene035561:CDS 40.0%
CGTGTCGAAGAAAAATCAGT+TGG - chr8.4:2043161-2043180 None:intergenic 40.0%
CTTAAGTTCATGTTACCAGG+TGG - chr8.4:2042828-2042847 None:intergenic 40.0%
GAAACGATGCAATGTCAAGA+TGG + chr8.4:2043203-2043222 MS.gene035561:CDS 40.0%
GAATGTTGAAGGGCTTACAA+GGG + chr8.4:2042176-2042195 MS.gene035561:CDS 40.0%
GAGCAATTGCTTGATGTAGA+TGG + chr8.4:2043452-2043471 MS.gene035561:CDS 40.0%
GATAAATGTTCCGGTGAAAC+TGG - chr8.4:2042806-2042825 None:intergenic 40.0%
GATTCTTCTTGATCCAGGTA+AGG - chr8.4:2042577-2042596 None:intergenic 40.0%
GCATGAGAGGAGAATAGTAT+GGG - chr8.4:2040333-2040352 None:intergenic 40.0%
GCTAACAAGAATAAAACCCG+TGG + chr8.4:2041797-2041816 MS.gene035561:intron 40.0%
GGACTTAAGTTCATGTTACC+AGG - chr8.4:2042831-2042850 None:intergenic 40.0%
GTAAACATGCCAGAAATGGA+TGG + chr8.4:2041409-2041428 MS.gene035561:CDS 40.0%
TACCAGGTGGATAAATGTTC+CGG - chr8.4:2042815-2042834 None:intergenic 40.0%
TATAGGAACCACTTGTAGCA+GGG - chr8.4:2043034-2043053 None:intergenic 40.0%
TCATCATCCCAAACTAAACG+AGG - chr8.4:2041926-2041945 None:intergenic 40.0%
TCTACTCTAGCCTGTTTAGA+TGG - chr8.4:2042495-2042514 None:intergenic 40.0%
TCTCAAAAGGCCATCTAAAC+AGG + chr8.4:2042482-2042501 MS.gene035561:CDS 40.0%
TCTCTTGCTTCTTCTAATGC+AGG + chr8.4:2043305-2043324 MS.gene035561:CDS 40.0%
TCTTGTGAAACCAATCCGAT+TGG + chr8.4:2041658-2041677 MS.gene035561:CDS 40.0%
TGAATGTTGAAGGGCTTACA+AGG + chr8.4:2042175-2042194 MS.gene035561:CDS 40.0%
TGCATGAGAGGAGAATAGTA+TGG - chr8.4:2040334-2040353 None:intergenic 40.0%
TGTGTGAGCTTACTGATGAA+GGG - chr8.4:2041475-2041494 None:intergenic 40.0%
TTAAGCTCTTCCAATCGGAT+TGG - chr8.4:2041671-2041690 None:intergenic 40.0%
TTATAGGAACCACTTGTAGC+AGG - chr8.4:2043035-2043054 None:intergenic 40.0%
TTCATTCTTTAGCGTGACGA+CGG + chr8.4:2041112-2041131 MS.gene035561:intron 40.0%
TTGACTGTCCTTTATAACGC+AGG + chr8.4:2041246-2041265 MS.gene035561:intron 40.0%
TTGGAGGTTGAAGATGTCTT+CGG + chr8.4:2040141-2040160 MS.gene035561:CDS 40.0%
! AAAGACACTGCTAGTCTGTT+TGG + chr8.4:2040886-2040905 MS.gene035561:intron 40.0%
! AAATGTGGCTAGCCATTTAC+AGG + chr8.4:2042200-2042219 MS.gene035561:CDS 40.0%
! AATGTAACATGTGTCGGTGT+TGG - chr8.4:2040533-2040552 None:intergenic 40.0%
! GAAGAAGCCTCGTTTAGTTT+GGG + chr8.4:2041916-2041935 MS.gene035561:intron 40.0%
! GACACATTAGTGTTGGAAGT+AGG + chr8.4:2041200-2041219 MS.gene035561:intron 40.0%
! GTGATGAAAGGTGTGATGAA+AGG + chr8.4:2041623-2041642 MS.gene035561:CDS 40.0%
! TCTTTTAACCCTGCTACAAG+TGG + chr8.4:2043023-2043042 MS.gene035561:CDS 40.0%
! TGAAGCGCTAGAATTGTTGA+GGG + chr8.4:2041351-2041370 MS.gene035561:CDS 40.0%
! TGAGCAAGTTGGACATGAAA+TGG + chr8.4:2041444-2041463 MS.gene035561:CDS 40.0%
! TGAGGCATCTGAGTGATTAA+TGG - chr8.4:2043371-2043390 None:intergenic 40.0%
! TTCGGAGAAGATTTTCCTGT+GGG + chr8.4:2040159-2040178 MS.gene035561:CDS 40.0%
! TTGAAGCGCTAGAATTGTTG+AGG + chr8.4:2041350-2041369 MS.gene035561:CDS 40.0%
! TTTCATGTCCAACTTGCTCA+AGG - chr8.4:2041444-2041463 None:intergenic 40.0%
!!! ATGTTTTGAGACTGAAGTGG+AGG - chr8.4:2042702-2042721 None:intergenic 40.0%
!!! GGAATGTTTTGAGACTGAAG+TGG - chr8.4:2042705-2042724 None:intergenic 40.0%
!!! TGTTTTGAGACTGAAGTGGA+GGG - chr8.4:2042701-2042720 None:intergenic 40.0%
ACTATTCTCCTCTCATGCAG+TGG + chr8.4:2040335-2040354 MS.gene035561:intron 45.0%
AGGGCTTACAAGGGAAAATG+TGG + chr8.4:2042185-2042204 MS.gene035561:CDS 45.0%
CCATCTGGATTGAACTTGAG+AGG + chr8.4:2042654-2042673 MS.gene035561:CDS 45.0%
CCTAAATTGAGAACTGAGCG+AGG - chr8.4:2043132-2043151 None:intergenic 45.0%
CCTCGCTCAGTTCTCAATTT+AGG + chr8.4:2043129-2043148 MS.gene035561:CDS 45.0%
CCTCTCAAGTTCAATCCAGA+TGG - chr8.4:2042657-2042676 None:intergenic 45.0%
GACAGTCAATTCACCCAATG+AGG - chr8.4:2041235-2041254 None:intergenic 45.0%
GATAGAGAGAGGGTGATGAA+AGG + chr8.4:2041611-2041630 MS.gene035561:CDS 45.0%
GGAAGAAGCCTCGTTTAGTT+TGG + chr8.4:2041915-2041934 MS.gene035561:intron 45.0%
GGAGATTTGTGTACCTTACC+TGG + chr8.4:2042561-2042580 MS.gene035561:CDS 45.0%
GTGTGTGAGCTTACTGATGA+AGG - chr8.4:2041476-2041495 None:intergenic 45.0%
TAATGCAGGCACTAGTTCCA+TGG + chr8.4:2043319-2043338 MS.gene035561:CDS 45.0%
TGAGCTTACTGATGAAGGGT+AGG - chr8.4:2041471-2041490 None:intergenic 45.0%
TTCAAGCTCCTTGAGCAAGT+TGG + chr8.4:2041433-2041452 MS.gene035561:CDS 45.0%
TTGGGCTGACACATTAGTGT+TGG + chr8.4:2041193-2041212 MS.gene035561:intron 45.0%
TTGGGGTACATTATGGACCA+TGG - chr8.4:2043339-2043358 None:intergenic 45.0%
! CTTCGGAGAAGATTTTCCTG+TGG + chr8.4:2040158-2040177 MS.gene035561:CDS 45.0%
ATAGCAGGACCTCCAACAGA+TGG - chr8.4:2042993-2043012 None:intergenic 50.0%
ATCTGTTGGAGGTCCTGCTA+TGG + chr8.4:2042992-2043011 MS.gene035561:CDS 50.0%
ATGATGAGGAACGCTCGACT+CGG + chr8.4:2041894-2041913 MS.gene035561:intron 50.0%
CACCGGAACATTTATCCACC+TGG + chr8.4:2042810-2042829 MS.gene035561:CDS 50.0%
CAGTCCTTCTCCATTGAGTG+AGG + chr8.4:2043064-2043083 MS.gene035561:CDS 50.0%
CTCACTCAATGGAGAAGGAC+TGG - chr8.4:2043066-2043085 None:intergenic 50.0%
GAGTTTGAACCACGACGTCT+TGG + chr8.4:2040461-2040480 MS.gene035561:intron 50.0%
TACACGCAGCAGCAAAACGT+GGG + chr8.4:2043257-2043276 MS.gene035561:CDS 50.0%
TAGCAGGACCTCCAACAGAT+GGG - chr8.4:2042992-2043011 None:intergenic 50.0%
TTACACGCAGCAGCAAAACG+TGG + chr8.4:2043256-2043275 MS.gene035561:CDS 50.0%
!! AAAGGGTGTTCGAACGTCAG+TGG + chr8.4:2042773-2042792 MS.gene035561:CDS 50.0%
!! GGCTTTGGAGAATCCTTTGG+AGG + chr8.4:2040125-2040144 MS.gene035561:CDS 50.0%
TCCTGTGGGAATGCGTGTTC+TGG + chr8.4:2040173-2040192 MS.gene035561:CDS 55.0%
GCCAGAACACGCATTCCCAC+AGG - chr8.4:2040177-2040196 None:intergenic 60.0%
! TGTCGGCTGAACGCACCATC+TGG + chr8.4:2042639-2042658 MS.gene035561:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 2040123 2043514 2040123 ID=MS.gene035561
chr8.4 mRNA 2040123 2043514 2040123 ID=MS.gene035561.t1;Parent=MS.gene035561
chr8.4 exon 2040123 2040261 2040123 ID=MS.gene035561.t1.exon1;Parent=MS.gene035561.t1
chr8.4 CDS 2040123 2040261 2040123 ID=cds.MS.gene035561.t1;Parent=MS.gene035561.t1
chr8.4 exon 2041329 2041481 2041329 ID=MS.gene035561.t1.exon2;Parent=MS.gene035561.t1
chr8.4 CDS 2041329 2041481 2041329 ID=cds.MS.gene035561.t1;Parent=MS.gene035561.t1
chr8.4 exon 2041594 2041746 2041594 ID=MS.gene035561.t1.exon3;Parent=MS.gene035561.t1
chr8.4 CDS 2041594 2041746 2041594 ID=cds.MS.gene035561.t1;Parent=MS.gene035561.t1
chr8.4 exon 2041933 2041992 2041933 ID=MS.gene035561.t1.exon4;Parent=MS.gene035561.t1
chr8.4 CDS 2041933 2041992 2041933 ID=cds.MS.gene035561.t1;Parent=MS.gene035561.t1
chr8.4 exon 2042145 2042221 2042145 ID=MS.gene035561.t1.exon5;Parent=MS.gene035561.t1
chr8.4 CDS 2042145 2042221 2042145 ID=cds.MS.gene035561.t1;Parent=MS.gene035561.t1
chr8.4 exon 2042468 2043514 2042468 ID=MS.gene035561.t1.exon6;Parent=MS.gene035561.t1
chr8.4 CDS 2042468 2043514 2042468 ID=cds.MS.gene035561.t1;Parent=MS.gene035561.t1
Gene Sequence

>MS.gene035561

ATGGCTTTGGAGAATCCTTTGGAGGTTGAAGATGTCTTCGGAGAAGATTTTCCTGTGGGAATGCGTGTTCTGGCTGTTGACGACGATCAAACTTGTCTCAAACTGTTGGAGAAGTTTCTTCTCATGTGCAAGTATAATGGTTCGTTTCCCTTTTTTCTTCTTTCTTTCAGTCAATTCATTTCTGCATTGATGATTCTTTGATGTTTTCCCATACTATTCTCCTCTCATGCAGTGGTATAAACTTATAATTGGAACTTTCATTGATATAGTAAACCCTTTTTTTAATCGGGGCAAATGTTACTTGTTAGTAATTTAGTGATGATTATATTAGTTTTTCAGAGTTTGAACCACGACGTCTTGGTTATAACTTATAAGCTTTGTAGTCGGTGCTGAGTGTGTGTTAGTATCCAACACCGACACATGTTACATTCAATCACTTGTTTTCTTAAAATATTTTCGATGTAATCTTGGCTACCCCAATTTTTTGTTTATTTCGGGTAAAATAAGTATACTTTTATGTTCAGCCACCCTAATAATATATGTTGACCTACCCCAAAGAAGTATATTTGCCAAAAAATTAGTAGAAACATAAAAGTTAAAATATTATTATTTAATTGATAATATTATTCGTCTTGTTTTGACATTTCCGGTCTTTACAACAAATATAGAGAGAACTTTGCTAGCAATGAAAATGGTAAGGTAAAAGTCGAAAATAAAAATAGAGGATGAGTTTCTTGCAAATAATTCAGTTGTTTTGATTGGAAAAGACACTGCTAGTCTGTTTGGTACAAATTATGAATGAATATGATTTTTTGAAATGTTGCTGAATTCAACTTTCCTAAATACTGTTTTCTCATTTTCTATTATTCTGTGCTGCTCTAATTTACATTTAAAATAAGTAAAAATTATTGCAAATTCTTGTAATGTAATTGTATATGTACATACACACACACATGAGTTGACGATTTCCATGATTTTAGATATAAAAATTCATTCTTTAGCGTGACGACGGTATTTCCTGTTCTCCTAAAGTTGAATTTTAAAATCTTTTAAAGATCACTACTATTTTATTGGGCTGACACATTAGTGTTGGAAGTAGGAATAATAGACCTCATTGGGTGAATTGACTGTCCTTTATAACGCAGGAAGCCTTTTTATTTTAAATGTCTCTCACTGAATTACTACTTTGATGCTTGGTTGTTGCAGTTACTACAACTATGAAGTCAGTTGAAGCGCTAGAATTGTTGAGGGAAAAGAGAGATATGTTTGACATTGTAATTAGTGATGTAAACATGCCAGAAATGGATGGATTCAAGCTCCTTGAGCAAGTTGGACATGAAATGGACCTACCCTTCATCAGTAAGCTCACACACCTTTGTTTTAGCATGTGTATTATTCAGCTAACTAAGATTCACTTCTTATATTCATATGGTATCTTTAAGTGATGATTTCTTTTTGTGTGATCATATAGTGTTGTCAGGAAATGATGATAGAGAGAGGGTGATGAAAGGTGTGATGAAAGGCGCTTGCGACTATCTTGTGAAACCAATCCGATTGGAAGAGCTTAAGAACATATGGCAACATGTAGTGAGAAAGAAGATTGAAAGTAAGGATCCGAATAAAGGTATCATTACTGATGGGATATGTAATCAAGACACCTCATCTGAAAATATTGCTAACAAGAATAAAACCCGTGGACAAAAAATAAAGGAACAAAGTGAGGATGAAGAAGATGAAGAAGAAAACGCAGAAGAAGATAATGGTAGAGAGAATGATGAGGAACGCTCGACTCGGAAGAAGCCTCGTTTAGTTTGGGATGATGAATTGCATAAAAAATTTGTATCTATAGTTAGTCAACTCGGCCTTGATAGTGAGTTTATGTGACAATCATTTTTTTTTTTTGTTACTTTACTAATTTGATTGGCTTTAAAACATGGAACCTTAACAAGACCATTTGATGATTGAAACTTACATATGGATATTATTGTTCCTAACATGTACTTTCTTCAACTGTTATTGCAGAGGCTTATCCTAAGAAAATATGTGACTTGATGAATGTTGAAGGGCTTACAAGGGAAAATGTGGCTAGCCATTTACAGGTACAAACTATAATTTCCCCTTTTGTCAAAATATTTCATACTAGTTTATCATTAAAATATTTGATCACCACATTTTTGCAAATATCGTCGAAGTTAAAGGGGATAATTTACTTTTAAACATATGCTTGTATGCAAATATGGTGTGAAAGTTTTTTATATCCATTATTCGTTGAAATTTCAACAGTCTCTTTTATGAAACATAAGTGGGAAAACTGCATGTAAGCTCGTTCTTGTTTTTTATTGCAGAAATACAAACTTAGTCTCAAAAGGCCATCTAAACAGGCTAGAGTAGATGCTGCATTAGATCCTCACCTACAAAAGAATTCAGTAGCTGGATATGGAGATTTGTGTACCTTACCTGGATCAAGAAGAATCTTAAGCAGCACATTGCCAACATATGCATCTAGTAACATGTATTGTCGGCTGAACGCACCATCTGGATTGAACTTGAGAGGAATGAGTTCTTCTGCATTAGTCCCTCCACTTCAGTCTCAAAACATTCCCAGTTACAAGCAGCCATTGTTTTTTGCAAGTGAAAACTCGAGTGCTTTAAAGGGTGTTCGAACGTCAGTGGAAATTAACCAGTTTCACCGGAACATTTATCCACCTGGTAACATGAACTTAAGTCCAATTGATGATTCAAGTGCATTTACAGATTCTTCTGGCTTCCAAGACATCAGAGCTAATGTTAATAATGCAAATAGTTTTCTTTCTTGCATCTCAAGCAATCACCTACAAACACATAATTCAAGAGCATTTATAAATCACCCATCTGTTGGAGGTCCTGCTATGGAACAAAAATCTTTTAACCCTGCTACAAGTGGTTCCTATAACTTTGCTTCCAGTCCTTCTCCATTGAGTGAGGATTTTAATAATGATCAAATATCTCACAATAGCTTAAAGTTTGCCTCGCTCAGTTCTCAATTTAGGAAAAGCCCAACTGATTTTTCTTCGACACGCGTCATTGATGTTCCTTTAGAGGAAACGATGCAATGTCAAGATGGATTACTTGGGAATGTTGTAACGGCTTCATGTTACACGCAGCAGCAAAACGTGGGTTCTTCCTTCAACAATACCTTGGATTCTCTTGCTTCTTCTAATGCAGGCACTAGTTCCATGGTCCATAATGTACCCCAAGCTAATAGTCCATTAATCACTCAGATGCCTCAAGTTGAGAAATTTTATTCAGATGAAAGAACTATGGAATCAAATGGTGGTGATTGCTTTTTTGAGCAATTGCTTGATGTAGATGGATTCGTTCAGAATAGTTGTTTGCCTTGGATGACACAATAA
Protein sequence

>MS.gene035561.t1

MALENPLEVEDVFGEDFPVGMRVLAVDDDQTCLKLLEKFLLMCKYNVTTTMKSVEALELLREKRDMFDIVISDVNMPEMDGFKLLEQVGHEMDLPFIMLSGNDDRERVMKGVMKGACDYLVKPIRLEELKNIWQHVVRKKIESKDPNKVWDDELHKKFVSIVSQLGLDKAYPKKICDLMNVEGLTRENVASHLQKYKLSLKRPSKQARVDAALDPHLQKNSVAGYGDLCTLPGSRRILSSTLPTYASSNMYCRLNAPSGLNLRGMSSSALVPPLQSQNIPSYKQPLFFASENSSALKGVRTSVEINQFHRNIYPPGNMNLSPIDDSSAFTDSSGFQDIRANVNNANSFLSCISSNHLQTHNSRAFINHPSVGGPAMEQKSFNPATSGSYNFASSPSPLSEDFNNDQISHNSLKFASLSSQFRKSPTDFSSTRVIDVPLEETMQCQDGLLGNVVTASCYTQQQNVGSSFNNTLDSLASSNAGTSSMVHNVPQANSPLITQMPQVEKFYSDERTMESNGGDCFFEQLLDVDGFVQNSCLPWMTQ