Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035710.t1 | AES92327.2 | 96.9 | 547 | 17 | 0 | 1 | 547 | 93 | 639 | 0.00E+00 | 1099.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035710.t1 | Q9XI84 | 24.1 | 278 | 192 | 9 | 144 | 410 | 47 | 316 | 9.4e-13 | 76.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035710.t1 | G7JVG8 | 96.9 | 547 | 17 | 0 | 1 | 547 | 93 | 639 | 0.0e+00 | 1099.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene035710.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035710.t1 | MTR_4g128240 | 96.892 | 547 | 17 | 0 | 1 | 547 | 93 | 639 | 0.0 | 1103 |
| MS.gene035710.t1 | MTR_3g082690 | 22.195 | 410 | 277 | 12 | 142 | 518 | 63 | 463 | 1.96e-18 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035710.t1 | AT2G18850 | 52.060 | 534 | 250 | 4 | 5 | 537 | 10 | 538 | 0.0 | 556 |
| MS.gene035710.t1 | AT2G18850 | 51.685 | 534 | 245 | 5 | 5 | 537 | 10 | 531 | 0.0 | 548 |
| MS.gene035710.t1 | AT3G07670 | 23.840 | 474 | 310 | 18 | 82 | 520 | 15 | 472 | 2.64e-21 | 97.8 |
| MS.gene035710.t1 | AT3G07670 | 24.843 | 318 | 199 | 11 | 234 | 520 | 26 | 334 | 5.95e-16 | 80.1 |
Find 118 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATTTAATTTCTGATTT+TGG | 0.061221 | 8.4:+6075336 | MS.gene035710:CDS |
| TGGTCTTCCCTCTCCATTAT+TGG | 0.251856 | 8.4:+6082947 | MS.gene035710:CDS |
| ATACATTATCTTCATGCTAT+TGG | 0.253277 | 8.4:-6081934 | None:intergenic |
| GTTTCAACTTCTTGCTGTAC+TGG | 0.267684 | 8.4:+6083919 | MS.gene035710:CDS |
| TAATTTGCAGGATTGAGTTT+TGG | 0.273161 | 8.4:+6078857 | MS.gene035710:intron |
| GTACTCCCTTCATATTGTTT+TGG | 0.278110 | 8.4:-6075362 | None:intergenic |
| CCGTATGATGTCATTCCTTT+AGG | 0.285448 | 8.4:+6082676 | MS.gene035710:CDS |
| GAAAATGAGCTAGAAGTTCT+TGG | 0.287372 | 8.4:+6083366 | MS.gene035710:CDS |
| CTGAGACAATATTGCTGTTA+TGG | 0.304721 | 8.4:+6076328 | MS.gene035710:CDS |
| GAGAGCCTCCAACTCATTCC+TGG | 0.355681 | 8.4:-6075226 | None:intergenic |
| TCAGAAATTACTACGATGTA+AGG | 0.359589 | 8.4:+6074795 | MS.gene035710:intron |
| AATCACAGCATATTCTATTA+TGG | 0.363717 | 8.4:+6082927 | MS.gene035710:CDS |
| AGTGCTCCTCAGGACCTTCT+TGG | 0.369830 | 8.4:+6074848 | MS.gene035710:CDS |
| ACATGTTTGATACCTCTTGC+TGG | 0.371762 | 8.4:+6081975 | MS.gene035710:CDS |
| TCCACAGTTGCAAGGAAACT+TGG | 0.382837 | 8.4:+6083344 | MS.gene035710:intron |
| TGACGACATGATGGACAGTA+TGG | 0.401765 | 8.4:+6083407 | MS.gene035710:CDS |
| ATCTCTGGAGGAAATATATC+AGG | 0.406816 | 8.4:-6081867 | None:intergenic |
| CTCCATTATTGGATCATCTT+CGG | 0.411384 | 8.4:+6082958 | MS.gene035710:CDS |
| CTATCAAGACCATGCCGATC+TGG | 0.413805 | 8.4:+6082571 | MS.gene035710:CDS |
| ATAGAATATGCTGTGATTGT+TGG | 0.416388 | 8.4:-6082922 | None:intergenic |
| CACCATGTGAGTCGTCCATT+TGG | 0.419396 | 8.4:-6082883 | None:intergenic |
| TGCCCACATATTACACACTA+TGG | 0.422578 | 8.4:+6082511 | MS.gene035710:CDS |
| TTCTCCCAAGTGTAAATCTC+TGG | 0.423678 | 8.4:-6081882 | None:intergenic |
| CTCCGAAGATGATCCAATAA+TGG | 0.426430 | 8.4:-6082960 | None:intergenic |
| AATTTCAATGAATTTGTTGA+AGG | 0.427682 | 8.4:-6082544 | None:intergenic |
| GCTTGTGCTTGAAACTGATA+TGG | 0.430090 | 8.4:+6075695 | MS.gene035710:CDS |
| AGATGTTGAAGGAGCAGGCC+GGG | 0.432091 | 8.4:+6075596 | MS.gene035710:intron |
| GCAGGCCGGGGAATGATAGC+AGG | 0.433260 | 8.4:+6075609 | MS.gene035710:CDS |
| AAAGTCTTTGTTTGCATCAC+AGG | 0.436089 | 8.4:-6082993 | None:intergenic |
| TTACTACGATGTAAGGGGTT+TGG | 0.439522 | 8.4:+6074802 | MS.gene035710:CDS |
| AAGCTCCTCAGAAATGATAA+TGG | 0.442652 | 8.4:-6075676 | None:intergenic |
| TTTCAACTTCTTGCTGTACT+GGG | 0.444329 | 8.4:+6083920 | MS.gene035710:CDS |
| CTCACATGGTGCGTGGTACC+TGG | 0.452049 | 8.4:+6082895 | MS.gene035710:CDS |
| TCCTTTCTTTGATGAAAAGA+AGG | 0.455845 | 8.4:+6073880 | MS.gene035710:CDS |
| GAATGAACTGCACAAGTGTT+TGG | 0.459339 | 8.4:+6083957 | MS.gene035710:CDS |
| TGTTTGGCTGAGGACATTGT+AGG | 0.459596 | 8.4:+6083973 | MS.gene035710:CDS |
| ACCCTTGCAGGATGTTTGTA+TGG | 0.460432 | 8.4:-6075299 | None:intergenic |
| TTGGTTCCTGCTTTGTGCAA+TGG | 0.460579 | 8.4:+6081840 | MS.gene035710:CDS |
| TTAATCTACACAGATGTTGA+AGG | 0.462913 | 8.4:+6075585 | MS.gene035710:intron |
| ATACATCTCAAGTGCTCCTC+AGG | 0.464633 | 8.4:+6074838 | MS.gene035710:CDS |
| AGGATAAACCTGTGTCCAAA+TGG | 0.465266 | 8.4:+6082868 | MS.gene035710:CDS |
| TTGAGATGTATCCATTCCTT+GGG | 0.465915 | 8.4:-6074826 | None:intergenic |
| CTTGAGATGTATCCATTCCT+TGG | 0.467813 | 8.4:-6074827 | None:intergenic |
| GTGAAGTCGATCTTGATAAC+AGG | 0.473430 | 8.4:+6083430 | MS.gene035710:CDS |
| TTCTGATGTTGATTCTATTG+AGG | 0.474846 | 8.4:+6082848 | MS.gene035710:CDS |
| TCAGTATCGTCTCTTGAAAG+AGG | 0.476597 | 8.4:+6076290 | MS.gene035710:intron |
| GAGAAGAACACCTGATTGAG+TGG | 0.477724 | 8.4:+6075409 | MS.gene035710:CDS |
| GCAGGGAAAGATCTAAAAGT+TGG | 0.483249 | 8.4:+6075627 | MS.gene035710:CDS |
| TGGGGACCAAAACAATATGA+AGG | 0.487327 | 8.4:+6075356 | MS.gene035710:CDS |
| GAAGAATGCTGCTTAAGCTA+TGG | 0.488333 | 8.4:+6082595 | MS.gene035710:CDS |
| CGTCTCTTGAAAGAGGTTGA+TGG | 0.489397 | 8.4:+6076297 | MS.gene035710:CDS |
| AGCAAGAAGTTGAAACAGAA+TGG | 0.489908 | 8.4:-6083912 | None:intergenic |
| GAAGCGATTACAATGCTAGA+TGG | 0.490904 | 8.4:+6078884 | MS.gene035710:CDS |
| CCTAAAGGAATGACATCATA+CGG | 0.505016 | 8.4:-6082676 | None:intergenic |
| GCTGTGATTGTTGGAGAGCC+AGG | 0.506463 | 8.4:-6082913 | None:intergenic |
| CAGCCCCAGGAATGAGTTGG+AGG | 0.515961 | 8.4:+6075221 | MS.gene035710:CDS |
| AGAGCCTCCAACTCATTCCT+GGG | 0.519139 | 8.4:-6075225 | None:intergenic |
| TCCTCCAGAGATTTACACTT+GGG | 0.521467 | 8.4:+6081878 | MS.gene035710:CDS |
| TACACAGATGTTGAAGGAGC+AGG | 0.521506 | 8.4:+6075591 | MS.gene035710:intron |
| AAAAGCCATGGTGTAAAGAC+AGG | 0.525288 | 8.4:+6075435 | MS.gene035710:CDS |
| ACGATATCATTCATCCAAGA+AGG | 0.525319 | 8.4:-6074862 | None:intergenic |
| AAATGTAATAAAATGAGAGC+TGG | 0.529710 | 8.4:-6082626 | None:intergenic |
| GGAAAACCATTGCACAAAGC+AGG | 0.530912 | 8.4:-6081846 | None:intergenic |
| CAGAAATTACTACGATGTAA+GGG | 0.531459 | 8.4:+6074796 | MS.gene035710:intron |
| ATGTAAGGGGTTTGGTCCCA+AGG | 0.532723 | 8.4:+6074810 | MS.gene035710:CDS |
| AGGGGTTTGGTCCCAAGGAA+TGG | 0.533877 | 8.4:+6074815 | MS.gene035710:CDS |
| CGTGTCCATTATCATTTCTG+AGG | 0.534034 | 8.4:+6075671 | MS.gene035710:CDS |
| CAGATGTTGAAGGAGCAGGC+CGG | 0.534056 | 8.4:+6075595 | MS.gene035710:intron |
| CTTTCCCTGCTATCATTCCC+CGG | 0.534319 | 8.4:-6075614 | None:intergenic |
| AAGAATGCTGCTTAAGCTAT+GGG | 0.536764 | 8.4:+6082596 | MS.gene035710:CDS |
| TTCTCAGAGAAAGCTACACC+TGG | 0.537382 | 8.4:+6083842 | MS.gene035710:intron |
| GCAAGAAGTTGAAACAGAAT+GGG | 0.537385 | 8.4:-6083911 | None:intergenic |
| TTACAGCCCCAGGAATGAGT+TGG | 0.537964 | 8.4:+6075218 | MS.gene035710:CDS |
| AGCAGCATTCTTCTCCAGAT+CGG | 0.542200 | 8.4:-6082585 | None:intergenic |
| ACAACTAGAAAGTGATTTGT+CGG | 0.544339 | 8.4:-6075268 | None:intergenic |
| GCACTCCTCGTCACTATCCA+TGG | 0.550146 | 8.4:-6073828 | None:intergenic |
| AGAGATAATGCAATCAAGAC+AGG | 0.553032 | 8.4:+6078922 | MS.gene035710:CDS |
| AGACATCTTTGACGACATGA+TGG | 0.557106 | 8.4:+6083398 | MS.gene035710:CDS |
| TTCCATACAAACATCCTGCA+AGG | 0.560101 | 8.4:+6075297 | MS.gene035710:CDS |
| TGAGTCGTCCATTTGGACAC+AGG | 0.564379 | 8.4:-6082876 | None:intergenic |
| CATTATCTCTTCAAACAGCA+AGG | 0.565535 | 8.4:-6078909 | None:intergenic |
| CTCAGAAATGATAATGGACA+CGG | 0.571725 | 8.4:-6075670 | None:intergenic |
| GCATGCTCAGTATGATGAGT+TGG | 0.574500 | 8.4:+6081821 | MS.gene035710:CDS |
| AGATGATCCAATAATGGAGA+GGG | 0.579934 | 8.4:-6082954 | None:intergenic |
| TTCCTCCAGAGATTTACACT+TGG | 0.582164 | 8.4:+6081877 | MS.gene035710:CDS |
| TTGCCATAGTGTGTAATATG+TGG | 0.582672 | 8.4:-6082514 | None:intergenic |
| AGAAGAACACCTGATTGAGT+GGG | 0.587488 | 8.4:+6075410 | MS.gene035710:CDS |
| AGAAATTACTACGATGTAAG+GGG | 0.589340 | 8.4:+6074797 | MS.gene035710:intron |
| GGGGACCAAAACAATATGAA+GGG | 0.590986 | 8.4:+6075357 | MS.gene035710:CDS |
| TCCATACAAACATCCTGCAA+GGG | 0.600641 | 8.4:+6075298 | MS.gene035710:CDS |
| ATGAAGATAATGTATTCAGA+TGG | 0.601114 | 8.4:+6081942 | MS.gene035710:CDS |
| GATGTTGAAGGAGCAGGCCG+GGG | 0.613770 | 8.4:+6075597 | MS.gene035710:intron |
| TGATTAAGAAAGCCAGCAAG+AGG | 0.615019 | 8.4:-6081987 | None:intergenic |
| TCCCAAGTGTAAATCTCTGG+AGG | 0.620554 | 8.4:-6081879 | None:intergenic |
| TTAATCCTGTCTTTACACCA+TGG | 0.622253 | 8.4:-6075440 | None:intergenic |
| CATTCTTCTCCAGATCGGCA+TGG | 0.630402 | 8.4:-6082580 | None:intergenic |
| GAGCCTCCAACTCATTCCTG+GGG | 0.630829 | 8.4:-6075224 | None:intergenic |
| TGCTGAAGACGATGCCTCGG+TGG | 0.645814 | 8.4:+6075191 | MS.gene035710:CDS |
| TGGACGACTCACATGGTGCG+TGG | 0.646565 | 8.4:+6082888 | MS.gene035710:CDS |
| CTAAAGGAATGACATCATAC+GGG | 0.653617 | 8.4:-6082675 | None:intergenic |
| AGGATGTTTGTATGGAAATG+TGG | 0.655140 | 8.4:-6075291 | None:intergenic |
| TCAGAAATGATAATGGACAC+GGG | 0.657279 | 8.4:-6075669 | None:intergenic |
| TGCCATAGTGTGTAATATGT+GGG | 0.660167 | 8.4:-6082513 | None:intergenic |
| CAGGCCGGGGAATGATAGCA+GGG | 0.660857 | 8.4:+6075610 | MS.gene035710:CDS |
| AAGATGATCCAATAATGGAG+AGG | 0.665042 | 8.4:-6082955 | None:intergenic |
| GTTCTTCTCTCTCACAACTG+TGG | 0.667911 | 8.4:-6075395 | None:intergenic |
| TGCAGCCATGGATAGTGACG+AGG | 0.679797 | 8.4:+6073823 | None:intergenic |
| GTCCAAATGGACGACTCACA+TGG | 0.681562 | 8.4:+6082881 | MS.gene035710:CDS |
| TCATACGGGTTGTCTCCTTG+AGG | 0.685652 | 8.4:-6082661 | None:intergenic |
| ATTGAGTGGGGTAAAAGCCA+TGG | 0.686263 | 8.4:+6075423 | MS.gene035710:CDS |
| TCTCAGAGAAAGCTACACCT+GGG | 0.686701 | 8.4:+6083843 | MS.gene035710:intron |
| GACGACATGATGGACAGTAT+GGG | 0.690308 | 8.4:+6083408 | MS.gene035710:CDS |
| GAAGAACACCTGATTGAGTG+GGG | 0.693168 | 8.4:+6075411 | MS.gene035710:CDS |
| ACTGCACAAGTGTTTGGCTG+AGG | 0.693657 | 8.4:+6083963 | MS.gene035710:CDS |
| ATTCATCCAAGAAGGTCCTG+AGG | 0.699592 | 8.4:-6074854 | None:intergenic |
| TGTATTCAGATGGAAAACTG+AGG | 0.723155 | 8.4:+6081952 | MS.gene035710:CDS |
| AAGAATCATACAGTTAAACG+AGG | 0.733021 | 8.4:+6074906 | MS.gene035710:CDS |
| GGGGCTGTAAAACTCCACCG+AGG | 0.821008 | 8.4:-6075205 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 6073830 | 6083999 | 6073830 | ID=MS.gene035710 |
| chr8.4 | mRNA | 6073830 | 6083999 | 6073830 | ID=MS.gene035710.t1;Parent=MS.gene035710 |
| chr8.4 | exon | 6073830 | 6073901 | 6073830 | ID=MS.gene035710.t1.exon1;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6073830 | 6073901 | 6073830 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6074799 | 6074927 | 6074799 | ID=MS.gene035710.t1.exon2;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6074799 | 6074927 | 6074799 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6075177 | 6075471 | 6075177 | ID=MS.gene035710.t1.exon3;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6075177 | 6075471 | 6075177 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6075598 | 6075716 | 6075598 | ID=MS.gene035710.t1.exon4;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6075598 | 6075716 | 6075598 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6076294 | 6076426 | 6076294 | ID=MS.gene035710.t1.exon5;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6076294 | 6076426 | 6076294 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6078867 | 6078943 | 6078867 | ID=MS.gene035710.t1.exon6;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6078867 | 6078943 | 6078867 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6081816 | 6082013 | 6081816 | ID=MS.gene035710.t1.exon7;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6081816 | 6082013 | 6081816 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6082508 | 6082697 | 6082508 | ID=MS.gene035710.t1.exon8;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6082508 | 6082697 | 6082508 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6082838 | 6083016 | 6082838 | ID=MS.gene035710.t1.exon9;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6082838 | 6083016 | 6082838 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6083351 | 6083451 | 6083351 | ID=MS.gene035710.t1.exon10;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6083351 | 6083451 | 6083351 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| chr8.4 | exon | 6083849 | 6083999 | 6083849 | ID=MS.gene035710.t1.exon11;Parent=MS.gene035710.t1 |
| chr8.4 | CDS | 6083849 | 6083999 | 6083849 | ID=cds.MS.gene035710.t1;Parent=MS.gene035710.t1 |
| Gene Sequence |
| Protein sequence |
