AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035959

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035959.t1 MTR_5g060990 75.197 254 16 2 1 209 49 300 6.90e-125 356
MS.gene035959.t1 MTR_3g010660 29.787 282 130 14 7 230 51 322 9.98e-18 80.9
MS.gene035959.t1 MTR_8g043960 30.252 238 107 9 35 230 93 313 1.03e-17 80.5
MS.gene035959.t1 MTR_7g088090 30.040 253 116 10 28 230 79 320 2.13e-17 79.7
MS.gene035959.t1 MTR_2g086390 33.152 184 92 8 30 206 72 231 3.72e-13 67.4
MS.gene035959.t1 MTR_2g086390 33.152 184 92 8 30 206 72 231 3.90e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACTCAAGTTCCATTTAT+TGG 0.183062 5.3:+48092629 MS.gene035959:CDS
TTTGGTTCAACTCCAATAAA+TGG 0.213001 5.3:-48092641 None:intergenic
GAGAACAAACTTATGGAAAT+TGG 0.227423 5.3:+48092848 MS.gene035959:CDS
TATTGGAGTTGAACCAAATT+TGG 0.228912 5.3:+48092646 MS.gene035959:CDS
TGTGAAGCCATGACCAAATT+TGG 0.273930 5.3:-48092659 None:intergenic
GGTTTATGAGAACAAACTTA+TGG 0.279232 5.3:+48092841 MS.gene035959:CDS
CATGCTAAGGCTATTAATAT+TGG 0.286901 5.3:+48092575 MS.gene035959:CDS
CTGAGCTAACACGCCGTAAT+TGG 0.291240 5.3:-48095908 None:intergenic
ATTAATTCTTCATTTCTATA+TGG 0.291977 5.3:+48092380 MS.gene035959:CDS
CAAGAAAATGTTCATGCTAT+TGG 0.325704 5.3:+48092602 MS.gene035959:CDS
CTCACTCCATACCTCATTGA+TGG 0.344051 5.3:-48092459 None:intergenic
AGATTTCGGTGTTGCAAATT+CGG 0.363775 5.3:+48092820 MS.gene035959:CDS
GAATTTGCAACACCGAAATC+TGG 0.383257 5.3:-48092818 None:intergenic
ATAGGGTTGTGTCCAGATTT+CGG 0.384705 5.3:+48092806 MS.gene035959:intron
GGTGCTGGTGGAAGCGGCGT+TGG 0.410177 5.3:+48092938 MS.gene035959:CDS
TTTCTATATGGCAACAATAG+TGG 0.416152 5.3:+48092392 MS.gene035959:CDS
AGAAATGAAGAATTAATATC+AGG 0.418535 5.3:-48092374 None:intergenic
GAGATCAAGGGCAAAGAAAC+AGG 0.423260 5.3:+48093048 MS.gene035959:CDS
GCGAATCTGCTGCGAGATCA+AGG 0.428490 5.3:+48093035 MS.gene035959:CDS
TCACTCCAACATACCAATTA+CGG 0.443876 5.3:+48095895 MS.gene035959:CDS
AAGGGAGGTGCTGGTGGAAG+CGG 0.452209 5.3:+48092932 MS.gene035959:CDS
CAATTTGCAACAATCAATGT+TGG 0.470147 5.3:+48092523 MS.gene035959:CDS
AAGAATTAATATCAGGTGTA+GGG 0.473272 5.3:-48092367 None:intergenic
TTGATCTCGCAGCAGATTCG+CGG 0.487789 5.3:-48093033 None:intergenic
AACAATAGTGGTAGTGGTAG+TGG 0.488487 5.3:+48092404 MS.gene035959:CDS
AATTTGCAACAATCAATGTT+GGG 0.488793 5.3:+48092524 MS.gene035959:CDS
GAAGAATTAATATCAGGTGT+AGG 0.490677 5.3:-48092368 None:intergenic
ATCTACATGTTCAGATTCAA+AGG 0.498171 5.3:+48092913 MS.gene035959:CDS
TCAGATTCAAAGGGAGGTGC+TGG 0.512549 5.3:+48092923 MS.gene035959:CDS
CAAGTATTCAGATGAGATGA+TGG 0.513206 5.3:+48092970 MS.gene035959:CDS
CTGGTGGAAGCGGCGTTGGA+AGG 0.516752 5.3:+48092942 MS.gene035959:CDS
AATGTTGGGAGAAACAACTT+TGG 0.520689 5.3:+48092538 MS.gene035959:CDS
ACAAAACCATCAATGAGGTA+TGG 0.522632 5.3:+48092453 MS.gene035959:CDS
TATGGCAACAATAGTGGTAG+TGG 0.526788 5.3:+48092398 MS.gene035959:CDS
GTTACTCAGAAATACCAATC+GGG 0.531399 5.3:+48092870 MS.gene035959:CDS
GGTTACTCAGAAATACCAAT+CGG 0.533750 5.3:+48092869 MS.gene035959:CDS
AGTTGAACCAAATTTGGTCA+TGG 0.554386 5.3:+48092652 MS.gene035959:CDS
TGTGTTGTCGTTGCCCCGAT+TGG 0.555305 5.3:-48092884 None:intergenic
ACACGCCGTAATTGGTATGT+TGG 0.562876 5.3:-48095900 None:intergenic
GATTCAAAGGGAGGTGCTGG+TGG 0.564466 5.3:+48092926 MS.gene035959:CDS
TGATGGAAAAGACTATTGAG+AGG 0.567476 5.3:+48092987 MS.gene035959:CDS
CGAATCTGCTGCGAGATCAA+GGG 0.577446 5.3:+48093036 MS.gene035959:CDS
TGAGAGGAGGCAGAAGCGAA+TGG 0.590383 5.3:+48093003 MS.gene035959:CDS
TCTACATGTTCAGATTCAAA+GGG 0.597077 5.3:+48092914 MS.gene035959:CDS
GTCATGGCTTCACAGCCAGA+AGG 0.598955 5.3:+48092668 MS.gene035959:CDS
ACATGTTCAGATTCAAAGGG+AGG 0.661738 5.3:+48092917 MS.gene035959:CDS
TGGAAAAGACTATTGAGAGG+AGG 0.667614 5.3:+48092990 MS.gene035959:CDS
GTGTAACAAAACCATCAATG+AGG 0.668143 5.3:+48092448 MS.gene035959:CDS
TTACTCAGAAATACCAATCG+GGG 0.689219 5.3:+48092871 MS.gene035959:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTATATTACAATTTGA+AGG + chr5.3:48095380-48095399 MS.gene035959:intron 10.0%
!! GTAATATAAATATAAATTTG+TGG - chr5.3:48095373-48095392 None:intergenic 10.0%
!!! TCTTTGAAAAAAATTATTTT+AGG + chr5.3:48095211-48095230 MS.gene035959:intron 10.0%
!!! TTTAACAAATCAATTTTATT+AGG + chr5.3:48095118-48095137 MS.gene035959:intron 10.0%
!! AAATAAAAATGATGAATTGT+TGG + chr5.3:48095732-48095751 MS.gene035959:intron 15.0%
!! ATTAATTCTTCATTTCTATA+TGG + chr5.3:48092380-48092399 MS.gene035959:CDS 15.0%
!! ATTTCCCTAAAAAAAATTAT+TGG + chr5.3:48095143-48095162 MS.gene035959:intron 15.0%
!! TTATTGTAAAAAAATTGTGA+TGG + chr5.3:48095336-48095355 MS.gene035959:intron 15.0%
!! TTTCCCTAAAAAAAATTATT+GGG + chr5.3:48095144-48095163 MS.gene035959:intron 15.0%
!!! AAACCCAATAATTTTTTTTA+GGG - chr5.3:48095150-48095169 None:intergenic 15.0%
!!! TAAACCCAATAATTTTTTTT+AGG - chr5.3:48095151-48095170 None:intergenic 15.0%
!! AACAAATAAAACAGAATGTA+TGG - chr5.3:48093261-48093280 None:intergenic 20.0%
!! AACCAACAAAAAAATGTAAT+TGG + chr5.3:48092485-48092504 MS.gene035959:CDS 20.0%
!! AAGTATCAAATAAAGAAGAA+TGG - chr5.3:48093152-48093171 None:intergenic 20.0%
!! ACCAACAAAAAAATGTAATT+GGG + chr5.3:48092486-48092505 MS.gene035959:CDS 20.0%
!! AGAAATGAAGAATTAATATC+AGG - chr5.3:48092377-48092396 None:intergenic 20.0%
!! AGCAAATAAAGGTTATAAAA+TGG - chr5.3:48095028-48095047 None:intergenic 20.0%
!! CATATTTACAGATAATAGAA+AGG - chr5.3:48095184-48095203 None:intergenic 20.0%
!! CTCAAGGAATTATATATATA+GGG + chr5.3:48093602-48093621 MS.gene035959:intron 20.0%
!! GCAAATAAAGGTTATAAAAT+GGG - chr5.3:48095027-48095046 None:intergenic 20.0%
!! TATTCTCTTATAACAAACAT+AGG - chr5.3:48094880-48094899 None:intergenic 20.0%
!! TCATTATTATTGATCAAGTT+TGG + chr5.3:48094009-48094028 MS.gene035959:intron 20.0%
!! TGAAAATATATCTTCTAATG+TGG + chr5.3:48095826-48095845 MS.gene035959:intron 20.0%
!! TTTATTAATATTGCAGAAAG+TGG + chr5.3:48095851-48095870 MS.gene035959:intron 20.0%
!!! ATGAAACTGTTATTTGAATT+TGG + chr5.3:48093465-48093484 MS.gene035959:intron 20.0%
!!! CTTCTTTATTTGATACTTTA+AGG + chr5.3:48093154-48093173 MS.gene035959:intron 20.0%
!!! TATATCTAATGTTTTCTCAT+AGG + chr5.3:48093639-48093658 MS.gene035959:intron 20.0%
!!! TTGGTATTTGAATTTTCAAT+GGG + chr5.3:48094424-48094443 MS.gene035959:intron 20.0%
!!! TTTGGTATTTGAATTTTCAA+TGG + chr5.3:48094423-48094442 MS.gene035959:intron 20.0%
! AAAAAATGTAATTGGGTCAA+TGG + chr5.3:48092493-48092512 MS.gene035959:CDS 25.0%
! AAGAATTAATATCAGGTGTA+GGG - chr5.3:48092370-48092389 None:intergenic 25.0%
! AATTAGAACACATTTACTTG+TGG + chr5.3:48093778-48093797 MS.gene035959:intron 25.0%
! AATTTGCAACAATCAATGTT+GGG + chr5.3:48092524-48092543 MS.gene035959:CDS 25.0%
! ACAAATTGAGATATTGCATT+AGG - chr5.3:48093331-48093350 None:intergenic 25.0%
! ACACATAAATAAGTTACAGA+TGG + chr5.3:48093178-48093197 MS.gene035959:intron 25.0%
! AGAAAAGATAGAAAATGATG+AGG - chr5.3:48095063-48095082 None:intergenic 25.0%
! ATATATAATTCCTTGAGGTT+TGG - chr5.3:48093599-48093618 None:intergenic 25.0%
! ATCAAAATCAACCGATAATA+GGG + chr5.3:48092789-48092808 MS.gene035959:intron 25.0%
! ATTTGAATTTGGATGCATTT+AGG + chr5.3:48093476-48093495 MS.gene035959:intron 25.0%
! CATATGATTTATCTTACAGT+TGG - chr5.3:48093757-48093776 None:intergenic 25.0%
! CCTATATATATAATTCCTTG+AGG - chr5.3:48093604-48093623 None:intergenic 25.0%
! CCTCAAGGAATTATATATAT+AGG + chr5.3:48093601-48093620 MS.gene035959:intron 25.0%
! GATGATAACTAAGTTATATG+AGG - chr5.3:48094219-48094238 None:intergenic 25.0%
! GTAATTAAGATTTGTCATTG+TGG + chr5.3:48093070-48093089 MS.gene035959:intron 25.0%
! GTTGTACACAAACAAATATT+AGG + chr5.3:48093975-48093994 MS.gene035959:intron 25.0%
! GTTGTACACAAAGAAATATT+AGG + chr5.3:48094806-48094825 MS.gene035959:intron 25.0%
! TATATAATTCCTTGAGGTTT+GGG - chr5.3:48093598-48093617 None:intergenic 25.0%
! TCAGTTGGAGAAAAAAAATA+CGG + chr5.3:48093208-48093227 MS.gene035959:intron 25.0%
! TGCATTCACAAAAAATTATG+TGG - chr5.3:48093432-48093451 None:intergenic 25.0%
!! CATAGGTAGAATCAATTTTT+GGG - chr5.3:48094913-48094932 None:intergenic 25.0%
!! CTGGTATTTGAATTTTCAAT+GGG + chr5.3:48094127-48094146 MS.gene035959:intron 25.0%
!! TCATAGGTAGAATCAATTTT+TGG - chr5.3:48094914-48094933 None:intergenic 25.0%
!! TCTGGTATTTGAATTTTCAA+TGG + chr5.3:48093871-48093890 MS.gene035959:intron 25.0%
!! TGGTATTTGAATTTTCAATG+GGG + chr5.3:48094128-48094147 MS.gene035959:intron 25.0%
!! TTAAACCTTTATGTGGTTTT+AGG + chr5.3:48095482-48095501 MS.gene035959:intron 25.0%
!! TTTTTGCTTAAACCTTTATG+TGG + chr5.3:48095475-48095494 MS.gene035959:intron 25.0%
!!! ACCCAATTACATTTTTTTGT+TGG - chr5.3:48092490-48092509 None:intergenic 25.0%
AAATCTTTGTGACATGAGAT+TGG + chr5.3:48094079-48094098 MS.gene035959:intron 30.0%
AACAAATAAATCCGAGTGAA+AGG - chr5.3:48095090-48095109 None:intergenic 30.0%
AATCTTTGTGACATGAGATT+GGG + chr5.3:48094080-48094099 MS.gene035959:intron 30.0%
AATTAGAACACACTTACTTG+TGG + chr5.3:48094329-48094348 MS.gene035959:intron 30.0%
AGGAAAAATTTATTCCCAAG+AGG + chr5.3:48094971-48094990 MS.gene035959:intron 30.0%
ATAACTATGTTACGAGGTTA+TGG - chr5.3:48093918-48093937 None:intergenic 30.0%
ATACGTGATAAACCAAATTG+AGG - chr5.3:48095630-48095649 None:intergenic 30.0%
ATATAATTCCTTGAGGTTTG+GGG - chr5.3:48093597-48093616 None:intergenic 30.0%
ATCTACATGTTCAGATTCAA+AGG + chr5.3:48092913-48092932 MS.gene035959:CDS 30.0%
ATCTCATGTCACAAAGATTT+CGG - chr5.3:48094079-48094098 None:intergenic 30.0%
ATTTGCATTTGCAATGGAAT+TGG - chr5.3:48092719-48092738 None:intergenic 30.0%
CAAGAAAATGTTCATGCTAT+TGG + chr5.3:48092602-48092621 MS.gene035959:CDS 30.0%
CAATTTGCAACAATCAATGT+TGG + chr5.3:48092523-48092542 MS.gene035959:CDS 30.0%
CATCAAAATCAACCGATAAT+AGG + chr5.3:48092788-48092807 MS.gene035959:intron 30.0%
CATGCTAAGGCTATTAATAT+TGG + chr5.3:48092575-48092594 MS.gene035959:CDS 30.0%
GAAAAGAAGAGAGCAAATAA+AGG - chr5.3:48095039-48095058 None:intergenic 30.0%
GAAACTCAAGTTCCATTTAT+TGG + chr5.3:48092629-48092648 MS.gene035959:CDS 30.0%
GAAGAATTAATATCAGGTGT+AGG - chr5.3:48092371-48092390 None:intergenic 30.0%
GAGAACAAACTTATGGAAAT+TGG + chr5.3:48092848-48092867 MS.gene035959:CDS 30.0%
GCTAATAGCTAACCTTTAAT+TGG - chr5.3:48095667-48095686 None:intergenic 30.0%
GGAGAAAAGGAAATACAAAA+AGG + chr5.3:48095503-48095522 MS.gene035959:intron 30.0%
GGGTACAAAATATAGTGTTA+AGG - chr5.3:48093577-48093596 None:intergenic 30.0%
GGTTATAAAATGGGAATTGT+AGG - chr5.3:48095018-48095037 None:intergenic 30.0%
GGTTTATGAGAACAAACTTA+TGG + chr5.3:48092841-48092860 MS.gene035959:CDS 30.0%
TAAATATTGAGGGTTTAGGA+GGG + chr5.3:48094544-48094563 MS.gene035959:intron 30.0%
TACGGAACTCAAATTGATAA+AGG + chr5.3:48093226-48093245 MS.gene035959:intron 30.0%
TACGTGATAAACCAAATTGA+GGG - chr5.3:48095629-48095648 None:intergenic 30.0%
TATTGGAGTTGAACCAAATT+TGG + chr5.3:48092646-48092665 MS.gene035959:CDS 30.0%
TCCTAAACCCTCAATATTTA+GGG - chr5.3:48094544-48094563 None:intergenic 30.0%
TCTACATGTTCAGATTCAAA+GGG + chr5.3:48092914-48092933 MS.gene035959:CDS 30.0%
TCTCTTTGTCATCACTTTAA+CGG - chr5.3:48094470-48094489 None:intergenic 30.0%
TGGATGATAATTAGGTTATG+AGG - chr5.3:48094514-48094533 None:intergenic 30.0%
TTATCACGTATGAGATTGAT+GGG + chr5.3:48095639-48095658 MS.gene035959:intron 30.0%
TTTATCACGTATGAGATTGA+TGG + chr5.3:48095638-48095657 MS.gene035959:intron 30.0%
TTTCTATATGGCAACAATAG+TGG + chr5.3:48092392-48092411 MS.gene035959:CDS 30.0%
TTTCTCCTAAAACCACATAA+AGG - chr5.3:48095490-48095509 None:intergenic 30.0%
! AAGGTTAGCTATTAGCAAAT+TGG + chr5.3:48095671-48095690 MS.gene035959:intron 30.0%
! ATGAAAACTGAGCTTCATTT+TGG - chr5.3:48095586-48095605 None:intergenic 30.0%
! TAATTTTCTTGCACATGCTA+AGG + chr5.3:48092562-48092581 MS.gene035959:CDS 30.0%
! TGAAAACTGAGCTTCATTTT+GGG - chr5.3:48095585-48095604 None:intergenic 30.0%
! TTTGGTTCAACTCCAATAAA+TGG - chr5.3:48092644-48092663 None:intergenic 30.0%
!!! GTCACATATATTTTGACCTA+AGG + chr5.3:48093349-48093368 MS.gene035959:intron 30.0%
!!! TTATGTGGTTTTAGGAGAAA+AGG + chr5.3:48095490-48095509 MS.gene035959:intron 30.0%
AAATATTGAGGGTTTAGGAG+GGG + chr5.3:48094545-48094564 MS.gene035959:intron 35.0%
AACTCCTTCTGATCTTATGT+TGG + chr5.3:48094947-48094966 MS.gene035959:intron 35.0%
AATGTTGGGAGAAACAACTT+TGG + chr5.3:48092538-48092557 MS.gene035959:CDS 35.0%
AATTAGAACACGCTTACTTG+TGG + chr5.3:48094032-48094051 MS.gene035959:intron 35.0%
ACAAAACCATCAATGAGGTA+TGG + chr5.3:48092453-48092472 MS.gene035959:CDS 35.0%
ACAGATGGACAAATTTCAGT+TGG + chr5.3:48093193-48093212 MS.gene035959:intron 35.0%
ACCCTAAATATTGAGGGTTT+AGG + chr5.3:48094540-48094559 MS.gene035959:intron 35.0%
ACTCCTTCTGATCTTATGTT+GGG + chr5.3:48094948-48094967 MS.gene035959:intron 35.0%
AGTTGAACCAAATTTGGTCA+TGG + chr5.3:48092652-48092671 MS.gene035959:CDS 35.0%
ATCAGAAGGAGTTACAAAGT+CGG - chr5.3:48094940-48094959 None:intergenic 35.0%
ATCGTGAACCCTAAATATTG+AGG + chr5.3:48094533-48094552 MS.gene035959:intron 35.0%
ATCTCCTTTCAACAAACCTT+AGG - chr5.3:48093368-48093387 None:intergenic 35.0%
ATTGGCATTTGCATTTGCAA+TGG - chr5.3:48092701-48092720 None:intergenic 35.0%
CAAGTATTCAGATGAGATGA+TGG + chr5.3:48092970-48092989 MS.gene035959:CDS 35.0%
CACTACCTACATTAGATTCT+CGG - chr5.3:48093399-48093418 None:intergenic 35.0%
CTAAATATTGAGGGTTTAGG+AGG + chr5.3:48094543-48094562 MS.gene035959:intron 35.0%
CTCCTAAACCCTCAATATTT+AGG - chr5.3:48094545-48094564 None:intergenic 35.0%
GATAAACCAAATTGAGGGAA+TGG - chr5.3:48095624-48095643 None:intergenic 35.0%
GATGATCTTGATGTTGTTGA+TGG - chr5.3:48092757-48092776 None:intergenic 35.0%
GGTTACTCAGAAATACCAAT+CGG + chr5.3:48092869-48092888 MS.gene035959:CDS 35.0%
GTGTAACAAAACCATCAATG+AGG + chr5.3:48092448-48092467 MS.gene035959:CDS 35.0%
GTTACTCAGAAATACCAATC+GGG + chr5.3:48092870-48092889 MS.gene035959:CDS 35.0%
GTTCACGATGGATGATAATT+AGG - chr5.3:48094522-48094541 None:intergenic 35.0%
TAAGACTTAGTCTCACATCA+TGG - chr5.3:48093833-48093852 None:intergenic 35.0%
TAAGATTTGTCATTGTGGTC+AGG + chr5.3:48093075-48093094 MS.gene035959:intron 35.0%
TATGTTACGAGGTTATGGTT+AGG - chr5.3:48093913-48093932 None:intergenic 35.0%
TCACTCCAACATACCAATTA+CGG + chr5.3:48095895-48095914 MS.gene035959:CDS 35.0%
TCGTGAACCCTAAATATTGA+GGG + chr5.3:48094534-48094553 MS.gene035959:intron 35.0%
TGATGGAAAAGACTATTGAG+AGG + chr5.3:48092987-48093006 MS.gene035959:CDS 35.0%
TGGATGATAACTATGTTACG+AGG - chr5.3:48093924-48093943 None:intergenic 35.0%
TTACTCAGAAATACCAATCG+GGG + chr5.3:48092871-48092890 MS.gene035959:CDS 35.0%
TTTGGATGAAGGGTTTATGA+GGG - chr5.3:48094758-48094777 None:intergenic 35.0%
! CTTTTCTTTCTCCTTTCACT+CGG + chr5.3:48095076-48095095 MS.gene035959:intron 35.0%
! TATAACGATTAGCTCGTTTG+CGG - chr5.3:48094158-48094177 None:intergenic 35.0%
! TCAATATTTAGGGTTCACGA+TGG - chr5.3:48094534-48094553 None:intergenic 35.0%
! TCTTTTCCATTCCCTCAATT+TGG + chr5.3:48095615-48095634 MS.gene035959:intron 35.0%
! TGTTGATGGATACTGATAGA+AGG - chr5.3:48092743-48092762 None:intergenic 35.0%
!! AGATTTCGGTGTTGCAAATT+CGG + chr5.3:48092820-48092839 MS.gene035959:CDS 35.0%
!! TTGACCTAAGGTTTGTTGAA+AGG + chr5.3:48093361-48093380 MS.gene035959:intron 35.0%
!! TTTAACGGTTAGCTTGTTTG+CGG - chr5.3:48094455-48094474 None:intergenic 35.0%
AAGATATGTGCGAGTAGTGT+CGG + chr5.3:48093514-48093533 MS.gene035959:intron 40.0%
AATATTGAGGGTTTAGGAGG+GGG + chr5.3:48094546-48094565 MS.gene035959:intron 40.0%
ACATGTTCAGATTCAAAGGG+AGG + chr5.3:48092917-48092936 MS.gene035959:CDS 40.0%
ATAGGGTTGTGTCCAGATTT+CGG + chr5.3:48092806-48092825 MS.gene035959:intron 40.0%
ATGGGAATTGTAGGTAGTAG+TGG - chr5.3:48095009-48095028 None:intergenic 40.0%
ATTAGCGTGTGTTTGGATGA+AGG - chr5.3:48094769-48094788 None:intergenic 40.0%
CCAAACACACGCTAATAGTT+GGG + chr5.3:48094773-48094792 MS.gene035959:intron 40.0%
CTTTGCATGCTCACATACTT+TGG + chr5.3:48094405-48094424 MS.gene035959:intron 40.0%
CTTTGCATGTACACATACTC+TGG + chr5.3:48094108-48094127 MS.gene035959:intron 40.0%
CTTTGCATTCTCACATACTC+TGG + chr5.3:48093853-48093872 MS.gene035959:intron 40.0%
GAATTTGCAACACCGAAATC+TGG - chr5.3:48092821-48092840 None:intergenic 40.0%
GATTTCGGCAAAGGTAATCT+TGG - chr5.3:48094064-48094083 None:intergenic 40.0%
GGTCTCTTAGTGATCATGAA+TGG - chr5.3:48093556-48093575 None:intergenic 40.0%
GTAACTGCAACGTTGATGTT+TGG + chr5.3:48095233-48095252 MS.gene035959:intron 40.0%
GTCGTCATAATTACCGTTGT+TGG + chr5.3:48094354-48094373 MS.gene035959:intron 40.0%
GTTACAAAGTCGGGTTTCAT+AGG - chr5.3:48094930-48094949 None:intergenic 40.0%
GTTTGGATGAAGGGTTTATG+AGG - chr5.3:48094759-48094778 None:intergenic 40.0%
TAAGACTCAGTCTCACATCA+CGG - chr5.3:48094385-48094404 None:intergenic 40.0%
TACATTAGATTCTCGGCAAC+AGG - chr5.3:48093392-48093411 None:intergenic 40.0%
TATGGCAACAATAGTGGTAG+TGG + chr5.3:48092398-48092417 MS.gene035959:CDS 40.0%
TCAGAAGGAGTTACAAAGTC+GGG - chr5.3:48094939-48094958 None:intergenic 40.0%
TCCAAACACACGCTAATAGT+TGG + chr5.3:48094772-48094791 MS.gene035959:intron 40.0%
TCTGGACACAACCCTATTAT+CGG - chr5.3:48092803-48092822 None:intergenic 40.0%
TGGAAAAGACTATTGAGAGG+AGG + chr5.3:48092990-48093009 MS.gene035959:CDS 40.0%
TGGGAATTGTAGGTAGTAGT+GGG - chr5.3:48095008-48095027 None:intergenic 40.0%
TGTCACAAAGATTTCGGCAA+AGG - chr5.3:48094073-48094092 None:intergenic 40.0%
TGTGAAGCCATGACCAAATT+TGG - chr5.3:48092662-48092681 None:intergenic 40.0%
TGTTGCCGAGAATCTAATGT+AGG + chr5.3:48093391-48093410 MS.gene035959:intron 40.0%
TTAGCGTGTGTTTGGATGAA+GGG - chr5.3:48094768-48094787 None:intergenic 40.0%
TTGGATGAAGGGTTTATGAG+GGG - chr5.3:48094757-48094776 None:intergenic 40.0%
! ATTTTGTACCCCAAACCTCA+AGG + chr5.3:48093586-48093605 MS.gene035959:intron 40.0%
! GGTTATGGTTAGGTTGTTTG+CGG - chr5.3:48093903-48093922 None:intergenic 40.0%
!! AACAATAGTGGTAGTGGTAG+TGG + chr5.3:48092404-48092423 MS.gene035959:CDS 40.0%
!! GAAGGCATTTGCATTTGCAA+TGG - chr5.3:48092725-48092744 None:intergenic 40.0%
!! GATTGATGGGCTCCAATTAA+AGG + chr5.3:48095652-48095671 MS.gene035959:intron 40.0%
ACACGCCGTAATTGGTATGT+TGG - chr5.3:48095903-48095922 None:intergenic 45.0%
ACACTATAGACCCAACCACT+AGG - chr5.3:48093955-48093974 None:intergenic 45.0%
ACATCCATGATGAACGAACG+AGG + chr5.3:48095540-48095559 MS.gene035959:intron 45.0%
AGATATGTGCGAGTAGTGTC+GGG + chr5.3:48093515-48093534 MS.gene035959:intron 45.0%
AGTCTCACATCATGGTGATC+CGG - chr5.3:48093825-48093844 None:intergenic 45.0%
AGTGTGTCCTAATGTGGATC+AGG - chr5.3:48093687-48093706 None:intergenic 45.0%
ATGACCTCGTTCGTTCATCA+TGG - chr5.3:48095547-48095566 None:intergenic 45.0%
CACTATAGACCCAACCACTA+GGG - chr5.3:48093954-48093973 None:intergenic 45.0%
CATCACGGTGATTCCAACAA+CGG - chr5.3:48094370-48094389 None:intergenic 45.0%
CCCAACTATTAGCGTGTGTT+TGG - chr5.3:48094776-48094795 None:intergenic 45.0%
CCTCCCAACATAAGATCAGA+AGG - chr5.3:48094954-48094973 None:intergenic 45.0%
CCTTCTGATCTTATGTTGGG+AGG + chr5.3:48094951-48094970 MS.gene035959:intron 45.0%
CTCACTCCATACCTCATTGA+TGG - chr5.3:48092462-48092481 None:intergenic 45.0%
GAGATCAAGGGCAAAGAAAC+AGG + chr5.3:48093048-48093067 MS.gene035959:CDS 45.0%
GATCCACATTAGGACACACT+AGG + chr5.3:48093687-48093706 MS.gene035959:intron 45.0%
GATCCTAGTGTGTCCTAATG+TGG - chr5.3:48093693-48093712 None:intergenic 45.0%
GCTCGACTAAGTTTGGAAGA+GGG - chr5.3:48093721-48093740 None:intergenic 45.0%
GGGAATTGTAGGTAGTAGTG+GGG - chr5.3:48095007-48095026 None:intergenic 45.0%
GGTTTATGAGGGGAAAGGAA+GGG - chr5.3:48094747-48094766 None:intergenic 45.0%
GTTGTCATGATTACCGTTGC+CGG + chr5.3:48093803-48093822 MS.gene035959:intron 45.0%
TGAAGGGTTTATGAGGGGAA+AGG - chr5.3:48094752-48094771 None:intergenic 45.0%
TGATGAACGAACGAGGTCAT+TGG + chr5.3:48095547-48095566 MS.gene035959:intron 45.0%
TGCAATGGTAACCAACCTTC+TGG - chr5.3:48092686-48092705 None:intergenic 45.0%
TTCCAAACTTAGTCGAGCGA+TGG + chr5.3:48093723-48093742 MS.gene035959:intron 45.0%
! TCACTAGCCTGATCCACATT+AGG + chr5.3:48093677-48093696 MS.gene035959:intron 45.0%
!! TAATATAAATATAAATTTGT+GGG - chr5.3:48095372-48095391 None:intergenic 5.0%
!!! TTTAAAAAATAATGAATTTT+CGG - chr5.3:48094301-48094320 None:intergenic 5.0%
!!! TTTTTTTATTAAAAAAATTG+TGG + chr5.3:48095302-48095321 MS.gene035959:intron 5.0%
ACATCATGGTGATCCGGCAA+CGG - chr5.3:48093819-48093838 None:intergenic 50.0%
AGCCATCGCTCGACTAAGTT+TGG - chr5.3:48093728-48093747 None:intergenic 50.0%
CATCGTGAACCCTAGTGGTT+GGG + chr5.3:48093942-48093961 MS.gene035959:intron 50.0%
CGAATCTGCTGCGAGATCAA+GGG + chr5.3:48093036-48093055 MS.gene035959:CDS 50.0%
CGCTCGACTAAGTTTGGAAG+AGG - chr5.3:48093722-48093741 None:intergenic 50.0%
CTGAGCTAACACGCCGTAAT+TGG - chr5.3:48095911-48095930 None:intergenic 50.0%
GATATGTGCGAGTAGTGTCG+GGG + chr5.3:48093516-48093535 MS.gene035959:intron 50.0%
GGGTTTATGAGGGGAAAGGA+AGG - chr5.3:48094748-48094767 None:intergenic 50.0%
GTGGATCAGGCTAGTGATGT+GGG - chr5.3:48093674-48093693 None:intergenic 50.0%
TCATCCATCGTGAACCCTAG+TGG + chr5.3:48093937-48093956 MS.gene035959:intron 50.0%
TGTGGATCAGGCTAGTGATG+TGG - chr5.3:48093675-48093694 None:intergenic 50.0%
TGTGGTCAGGCATAAGCATC+AGG + chr5.3:48093088-48093107 MS.gene035959:intron 50.0%
TTGATCTCGCAGCAGATTCG+CGG - chr5.3:48093036-48093055 None:intergenic 50.0%
! TCAGATTCAAAGGGAGGTGC+TGG + chr5.3:48092923-48092942 MS.gene035959:CDS 50.0%
CCAACCACTAGGGTTCACGA+TGG - chr5.3:48093944-48093963 None:intergenic 55.0%
CCATCGTGAACCCTAGTGGT+TGG + chr5.3:48093941-48093960 MS.gene035959:intron 55.0%
GCGAATCTGCTGCGAGATCA+AGG + chr5.3:48093035-48093054 MS.gene035959:CDS 55.0%
GGGGCAACACAGTACCTCTT+GGG - chr5.3:48094988-48095007 None:intergenic 55.0%
GGTTTAGGAGGGGGAAAGGA+GGG + chr5.3:48094555-48094574 MS.gene035959:intron 55.0%
GTCATGGCTTCACAGCCAGA+AGG + chr5.3:48092668-48092687 MS.gene035959:CDS 55.0%
GTTTAGGAGGGGGAAAGGAG+GGG + chr5.3:48094556-48094575 MS.gene035959:intron 55.0%
TGAGAGGAGGCAGAAGCGAA+TGG + chr5.3:48093003-48093022 MS.gene035959:CDS 55.0%
TGAGGGTTTAGGAGGGGGAA+AGG + chr5.3:48094551-48094570 MS.gene035959:intron 55.0%
TGGCTTCACAGCCAGAAGGT+TGG + chr5.3:48092672-48092691 MS.gene035959:intron 55.0%
TGGGGCAACACAGTACCTCT+TGG - chr5.3:48094989-48095008 None:intergenic 55.0%
TGTGTTGTCGTTGCCCCGAT+TGG - chr5.3:48092887-48092906 None:intergenic 55.0%
!! GATTCAAAGGGAGGTGCTGG+TGG + chr5.3:48092926-48092945 MS.gene035959:CDS 55.0%
GGGTTTAGGAGGGGGAAAGG+AGG + chr5.3:48094554-48094573 MS.gene035959:intron 60.0%
!! AAGGGAGGTGCTGGTGGAAG+CGG + chr5.3:48092932-48092951 MS.gene035959:CDS 60.0%
! CTGGTGGAAGCGGCGTTGGA+AGG + chr5.3:48092942-48092961 MS.gene035959:CDS 65.0%
!! GGTGCTGGTGGAAGCGGCGT+TGG + chr5.3:48092938-48092957 MS.gene035959:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 48092332 48095941 48092332 ID=MS.gene035959
chr5.3 mRNA 48092332 48095941 48092332 ID=MS.gene035959.t1;Parent=MS.gene035959
chr5.3 exon 48092332 48092689 48092332 ID=MS.gene035959.t1.exon1;Parent=MS.gene035959.t1
chr5.3 CDS 48092332 48092689 48092332 ID=cds.MS.gene035959.t1;Parent=MS.gene035959.t1
chr5.3 exon 48092810 48093069 48092810 ID=MS.gene035959.t1.exon2;Parent=MS.gene035959.t1
chr5.3 CDS 48092810 48093069 48092810 ID=cds.MS.gene035959.t1;Parent=MS.gene035959.t1
chr5.3 exon 48095867 48095941 48095867 ID=MS.gene035959.t1.exon3;Parent=MS.gene035959.t1
chr5.3 CDS 48095867 48095941 48095867 ID=cds.MS.gene035959.t1;Parent=MS.gene035959.t1
Gene Sequence

>MS.gene035959

ATGATTTCAACTGAAAGTGATCAACTTATACAAAACCCTACACCTGATATTAATTCTTCATTTCTATATGGCAACAATAGTGGTAGTGGTAGTGGTAATAATTTGAATGAAACAGTGTGTAACAAAACCATCAATGAGGTATGGAGTGAGATTAACCAACAAAAAAATGTAATTGGGTCAATGGATCATAACAATTTGCAACAATCAATGTTGGGAGAAACAACTTTGGATAATTTTCTTGCACATGCTAAGGCTATTAATATTGGAAATCAAGAAAATGTTCATGCTATTGGTGATGAAACTCAAGTTCCATTTATTGGAGTTGAACCAAATTTGGTCATGGCTTCACAGCCAGAAGGTTGGTTACCATTGCAAATGCAAATGCCAATTCCATTGCAAATGCAAATGCCTTCTATCAGTATCCATCAACAACATCAAGATCATCATCATCGTCTTCATCAAAATCAACCGATAATAGGGTTGTGTCCAGATTTCGGTGTTGCAAATTCGGTTTATGAGAACAAACTTATGGAAATTGGTTACTCAGAAATACCAATCGGGGCAACGACAACACATTTATCATCTACATGTTCAGATTCAAAGGGAGGTGCTGGTGGAAGCGGCGTTGGAAGGAAGCACAAGTATTCAGATGAGATGATGGAAAAGACTATTGAGAGGAGGCAGAAGCGAATGGCGAAGAACCGCGAATCTGCTGCGAGATCAAGGGCAAAGAAACAGGTAATTAAGATTTGTCATTGTGGTCAGGCATAAGCATCAGGTTACTCAATGATATCTTATTCTACACATTGTATATTTACCATTCTTCTTTATTTGATACTTTAAGGAACACATAAATAAGTTACAGATGGACAAATTTCAGTTGGAGAAAAAAAATACGGAACTCAAATTGATAAAGGTACACACTTCCATACATTCTGTTTTATTTGTTTGTGTTGTATTGATCATAATTTATTATATATACTTTATTGTGATGTTCCTAATGCAATATCTCAATTTGTCACATATATTTTGACCTAAGGTTTGTTGAAAGGAGATTCCTGTTGCCGAGAATCTAATGTAGGTAGTGTATTTATGTACCACATAATTTTTTGTGAATGCATTTATGTACTGAGATGAAACTGTTATTTGAATTTGGATGCATTTAGGTGCTTACATGTATGAAAGATATGTGCGAGTAGTGTCGGGGCGTCAATAGTTTAACCATTCATGATCACTAAGAGACCTTAACACTATATTTTGTACCCCAAACCTCAAGGAATTATATATATAGGGCATCTCTCTTATACTATATCTAATGTTTTCTCATAGGTGTGAGTCACCCACATCACTAGCCTGATCCACATTAGGACACACTAGGATCGCGCTCCCTCTTCCAAACTTAGTCGAGCGATGGCTCTGATACCAACTGTAAGATAAATCATATGAAATTAGAACACATTTACTTGTGGAAGTTGTCATGATTACCGTTGCCGGATCACCATGATGTGAGACTAAGTCTTACTTTGCATTCTCACATACTCTGGTATTTGAATTTTCAAAAGAGCATACCGCAAACAACCTAACCATAACCTCGTAACATAGTTATCATCCATCGTGAACCCTAGTGGTTGGGTCTATAGTGTGTTGTACACAAACAAATATTAGGTGTGCAAAAATTCATTATTATTGATCAAGTTATGAATTAGAACACGCTTACTTGTGGAAGTCGCCAAGATTACCTTTGCCGAAATCTTTGTGACATGAGATTGGGTCTTGCTTTGCATGTACACATACTCTGGTATTTGAATTTTCAATGGGGCAAACCGCAAACGAGCTAATCGTTATAGAGTGATGACAAAGAGAACACAATCTCTATGTTTATAACCTCATATAACTTAGTTATCATCCATCGTGAACCCTAGTGGTTGGGTCTATAGTGTGTTGTACTCAAACAAATATTACGTGTCCGAAAATTCATTATTTTTTAAAAGTTAAGAAATTAGAACACACTTACTTGTGGAAGTCGTCATAATTACCGTTGTTGGAATCACCGTGATGTGAGACTGAGTCTTACTTTGCATGCTCACATACTTTGGTATTTGAATTTTCAATGGGGAATACCGCAAACAAGCTAACCGTTAAAGTGATGACAAAGAGAACATAATCTCTATGTTTATAACCTCATAACCTAATTATCATCCATCGTGAACCCTAAATATTGAGGGTTTAGGAGGGGGAAAGGAGGGGAGAACTCACTTTTATTTTTTAAATTACGAACAATGTAAAATGTTTTTAAAAAATAAATTAAGTGTTTTTGAACTATTATATATATGATCATTTGTCATGTTATTAATTCAATTTTCTAAAAATCAATTTATAATGTCTATAATTTAAAATAGAAAACTAAACTCTCCCTTCCTTTCCCCTCATAAACCCTTCATCCAAACACACGCTAATAGTTGGGTCTATAGTGTGTTGTACACAAAGAAATATTAGGTGTACAAAAATTCATTATTATTGATCAAGTTTGGCAGAAATAAGTATTCCTATGTTTGTTATAAGAGAATAACATTTTGTTCCCAAAAATTGATTCTACCTATGAAACCCGACTTTGTAACTCCTTCTGATCTTATGTTGGGAGGAAAAATTTATTCCCAAGAGGTACTGTGTTGCCCCACTACTACCTACAATTCCCATTTTATAACCTTTATTTGCTCTCTTCTTTTCACCTCATCATTTTCTATCTTTTCTTTCTCCTTTCACTCGGATTTATTTGTTTGTGTTTATTTAACAAATCAATTTTATTAGGTTATTTCCCTAAAAAAAATTATTGGGTTTATTATTATAATCCTTTCTATTATCTGTAAATATGTTAGTTTTCTTTGAAAAAAATTATTTTAGGTAACTGCAACGTTGATGTTTGGTTTGAAAAATTAATTGTGAGTAAAACATTTGAATTAATAAATGTTGTTTTTTTATTAAAAAAATTGTGGCTCTTTTTTTTTTATTGTAAAAAAATTGTGATGGAGAATTTAAACCCACAAATTTATATTTATATTACAATTTGAAGGTTTTTATAATAATAGTTTAGACTTTTTTTTTTTCTTTTTCTTATAGTATAGACTTTTTTGTTCTTTTTCTTTTTTTTGCTTAAACCTTTATGTGGTTTTAGGAGAAAAGGAAATACAAAAAGGAGAGATGAGCAATAACATCCATGATGAACGAACGAGGTCATTGGTCAATGAGTTTACCCAAAATGAAGCTCAGTTTTCATGCTTTCATTTCTTTTCCATTCCCTCAATTTGGTTTATCACGTATGAGATTGATGGGCTCCAATTAAAGGTTAGCTATTAGCAAATTGGAGTTCAAATTAAAAAGAAAAAGAAGATTAAATATATAAAAATAAAAATGATGAATTGTTGGTTGATTAGATTAGATTTATTTTCATCTACATATATATTGATATTTATCTTAATGTGTTTTTCTTCTTTATATGAAAATATATCTTCTAATGTGGTTTTTATTAATATTGCAGAAAGTGGAAGAACAATTATTTTCAAATCTCACTCCAACATACCAATTACGGCGTGTTAGCTCAGCTAAATTCTAG
Protein sequence

>MS.gene035959.t1

MISTESDQLIQNPTPDINSSFLYGNNSGSGSGNNLNETVCNKTINEVWSEINQQKNVIGSMDHNNLQQSMLGETTLDNFLAHAKAINIGNQENVHAIGDETQVPFIGVEPNLVMASQPEGLCPDFGVANSVYENKLMEIGYSEIPIGATTTHLSSTCSDSKGGAGGSGVGRKHKYSDEMMEKTIERRQKRMAKNRESAARSRAKKQKVEEQLFSNLTPTYQLRRVSSAKF