AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035960.t1 MTR_5g060950 97.486 358 9 0 16 373 1 358 0.0 727
MS.gene035960.t1 MTR_3g073560 66.379 348 111 3 16 361 1 344 9.27e-158 456
MS.gene035960.t1 MTR_3g087940 49.587 363 172 3 4 361 2 358 3.63e-117 344
MS.gene035960.t1 MTR_8g091650 47.126 348 177 4 14 361 13 353 4.16e-99 298
MS.gene035960.t1 MTR_8g091650 43.307 381 179 4 14 361 13 389 2.83e-94 287
MS.gene035960.t1 MTR_8g091650 52.347 277 131 1 85 361 5 280 7.02e-93 280
MS.gene035960.t1 MTR_7g092750 47.333 300 151 2 69 361 60 359 5.90e-91 278
MS.gene035960.t1 MTR_8g040120 52.613 287 125 3 82 361 181 463 6.94e-90 278
MS.gene035960.t1 MTR_7g092750 47.333 300 151 2 69 361 161 460 1.14e-89 278
MS.gene035960.t1 MTR_7g089800 51.568 287 128 4 82 361 138 420 1.37e-89 276
MS.gene035960.t1 MTR_7g089800 51.568 287 128 4 82 361 167 449 2.25e-89 276
MS.gene035960.t1 MTR_2g086420 51.786 280 128 2 82 361 194 466 3.92e-89 277
MS.gene035960.t1 MTR_2g086420 51.786 280 128 2 82 361 193 465 4.48e-89 277
MS.gene035960.t1 MTR_4g053250 45.981 311 155 5 52 361 160 458 3.14e-83 262
MS.gene035960.t1 MTR_5g054900 45.302 298 152 2 71 361 57 350 1.54e-81 253
MS.gene035960.t1 MTR_5g054900 45.302 298 152 2 71 361 138 431 1.44e-80 253
MS.gene035960.t1 MTR_5g054900 44.631 298 150 3 71 361 57 346 1.45e-77 243
MS.gene035960.t1 MTR_7g092750 43.333 300 137 3 69 361 161 434 1.17e-76 243
MS.gene035960.t1 MTR_5g054900 44.631 298 150 3 71 361 168 457 2.64e-76 243
MS.gene035960.t1 MTR_1g111310 46.181 288 151 2 77 360 416 703 3.63e-74 244
MS.gene035960.t1 MTR_1g111310 44.912 285 147 2 77 358 49 326 1.26e-66 223
MS.gene035960.t1 MTR_0126s0080 40.118 339 177 6 4 317 122 459 1.05e-66 218
MS.gene035960.t1 MTR_3g044820 42.286 175 86 3 77 240 40 210 2.20e-42 147
MS.gene035960.t1 MTR_1g090370 31.000 200 115 6 163 357 47 228 1.27e-14 72.8
MS.gene035960.t1 MTR_1g111320 64.000 50 18 0 82 131 146 195 1.27e-12 67.0
MS.gene035960.t1 MTR_7g068860 25.698 179 117 3 188 354 45 219 2.86e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035960.t1 AT1G77920 52.520 377 149 6 1 361 1 363 9.90e-123 359
MS.gene035960.t1 AT1G22070 50.538 372 159 4 14 365 17 383 4.48e-118 347
MS.gene035960.t1 AT5G10030 50.416 361 173 3 4 361 2 359 4.50e-116 342
MS.gene035960.t1 AT5G10030 50.416 361 173 3 4 361 2 359 4.50e-116 342
MS.gene035960.t1 AT5G10030 50.416 361 173 3 4 361 2 359 4.50e-116 342
MS.gene035960.t1 AT5G10030 50.416 361 173 3 4 361 2 359 4.50e-116 342
MS.gene035960.t1 AT5G10030 50.416 361 173 3 4 361 2 359 4.50e-116 342
MS.gene035960.t1 AT5G65210 48.919 370 182 4 4 369 2 368 5.60e-116 342
MS.gene035960.t1 AT5G65210 48.919 370 182 4 4 369 2 368 5.60e-116 342
MS.gene035960.t1 AT5G65210 48.919 370 182 4 4 369 2 368 5.60e-116 342
MS.gene035960.t1 AT5G65210 48.919 370 182 4 4 369 2 368 5.60e-116 342
MS.gene035960.t1 AT5G65210 48.919 370 182 4 4 369 2 368 5.60e-116 342
MS.gene035960.t1 AT5G65210 48.919 370 182 4 4 369 2 368 5.60e-116 342
MS.gene035960.t1 AT5G65210 53.512 299 135 2 72 369 3 298 1.24e-105 313
MS.gene035960.t1 AT1G08320 47.706 327 152 6 46 361 8 326 2.10e-92 281
MS.gene035960.t1 AT1G08320 47.706 327 152 6 46 361 8 326 2.10e-92 281
MS.gene035960.t1 AT1G08320 47.706 327 152 6 46 361 132 450 7.57e-91 281
MS.gene035960.t1 AT1G08320 47.706 327 152 6 46 361 132 450 7.57e-91 281
MS.gene035960.t1 AT1G08320 47.706 327 152 6 46 361 132 450 7.57e-91 281
MS.gene035960.t1 AT5G06839 45.614 342 165 5 37 361 75 412 2.19e-87 270
MS.gene035960.t1 AT5G06839 47.541 305 145 2 72 361 151 455 6.04e-87 270
MS.gene035960.t1 AT3G12250 49.650 286 134 2 82 361 19 300 2.97e-86 263
MS.gene035960.t1 AT5G06839 44.898 343 167 5 37 361 75 413 3.69e-86 267
MS.gene035960.t1 AT3G12250 49.650 286 134 2 82 361 46 327 4.52e-86 264
MS.gene035960.t1 AT3G12250 49.650 286 134 2 82 361 46 327 4.52e-86 264
MS.gene035960.t1 AT3G12250 49.650 286 134 2 82 361 46 327 4.52e-86 264
MS.gene035960.t1 AT3G12250 49.650 286 134 2 82 361 46 327 4.52e-86 264
MS.gene035960.t1 AT3G12250 49.650 286 134 2 82 361 40 321 4.60e-86 264
MS.gene035960.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.43e-86 264
MS.gene035960.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.43e-86 264
MS.gene035960.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.43e-86 264
MS.gene035960.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.43e-86 264
MS.gene035960.t1 AT5G06950 48.951 286 136 2 82 361 46 327 5.43e-86 264
MS.gene035960.t1 AT3G12250 49.650 286 134 2 82 361 71 352 1.93e-85 263
MS.gene035960.t1 AT5G06960 48.601 286 137 2 82 361 26 307 1.67e-83 256
MS.gene035960.t1 AT5G06960 48.601 286 137 2 82 361 46 327 1.88e-83 257
MS.gene035960.t1 AT5G06960 48.601 286 137 2 82 361 46 327 1.88e-83 257
MS.gene035960.t1 AT1G68640 44.295 298 154 3 72 361 156 449 4.60e-72 232
MS.gene035960.t1 AT1G58330 30.412 194 112 6 178 354 36 223 3.08e-14 72.0

Find 66 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTGGATGTCGGTTATA+TGG 0.193824 5.3:-48064232 MS.gene035960:CDS
TCTTTGTTTGAAATTGAATA+TGG 0.229069 5.3:-48064102 MS.gene035960:CDS
TTTATACAGAAGCAGTTTAT+TGG 0.255260 5.3:-48064247 MS.gene035960:CDS
CAAGCTGATCCCTTGGATTT+CGG 0.267505 5.3:-48063279 MS.gene035960:CDS
TTGCAGCTTCTGCTTTCATT+TGG 0.310238 5.3:+48063956 None:intergenic
TGCGCAAGGCTTGTTGGCTA+TGG 0.324120 5.3:-48062965 MS.gene035960:CDS
ATCCTCCTATCCAAAGAAAA+AGG 0.354680 5.3:+48063881 None:intergenic
TTATGTTCATCTTCAACATT+GGG 0.356119 5.3:+48065154 None:intergenic
CCCTATTGATAGTGCATCTT+CGG 0.357661 5.3:+48063338 None:intergenic
TCATTCAAATTCTCAAGTTT+CGG 0.363992 5.3:+48063408 None:intergenic
GAAGCTCATGCAGTTGGAAT+TGG 0.366242 5.3:-48064398 MS.gene035960:CDS
CTTATGTTCATCTTCAACAT+TGG 0.379233 5.3:+48065153 None:intergenic
TTTGGAAAAGATTTGAGTAT+TGG 0.386257 5.3:+48063974 None:intergenic
GCGCAAGGCTTGTTGGCTAT+GGG 0.386417 5.3:-48062964 MS.gene035960:CDS
CTCAACTAATCGCGCCGAAA+AGG 0.387136 5.3:-48065263 MS.gene035960:CDS
GTCGGTTATATGGGATCGTC+TGG 0.405902 5.3:-48064222 MS.gene035960:CDS
ACAGGCTTATGTTCAACAAC+TGG 0.406023 5.3:-48064434 MS.gene035960:intron
GCCGAAGATGCACTATCAAT+AGG 0.415437 5.3:-48063339 MS.gene035960:CDS
CTTTACAGGCAGATCACCTT+AGG 0.419843 5.3:-48063035 MS.gene035960:intron
TTATTGGATGTCGGTTATAT+GGG 0.421066 5.3:-48064231 MS.gene035960:CDS
CCTCCCACATAGTCACTTGA+AGG 0.428980 5.3:+48065220 None:intergenic
TCAAGCTGCGCAAGGCTTGT+TGG 0.435023 5.3:-48062971 MS.gene035960:CDS
AAGGTTCTCTTGATTCTTGA+GGG 0.435161 5.3:+48065005 None:intergenic
CCCTTGGATTTCGGAAACTA+CGG 0.436857 5.3:-48063270 MS.gene035960:CDS
CAAGAATCAAGAGAACCTTC+TGG 0.437311 5.3:-48065001 MS.gene035960:CDS
GACAAAGGAGAAGCAGTAGA+AGG 0.441538 5.3:-48063225 MS.gene035960:CDS
TATCGAGAAAACAAGAAAAC+AGG 0.442585 5.3:-48064374 MS.gene035960:intron
AATTGTATCACCTTAGGAAC+AGG 0.450989 5.3:+48064953 None:intergenic
CAACATTCAAGCTGATCCCT+TGG 0.462368 5.3:-48063286 MS.gene035960:CDS
TCTTAATTTGAGTGTTGAGT+AGG 0.465347 5.3:+48062874 None:intergenic
GAAGGTTCTCTTGATTCTTG+AGG 0.474792 5.3:+48065004 None:intergenic
TGTTGTTGATCATACTCTGC+TGG 0.478454 5.3:+48063304 None:intergenic
TCTATTTAATCTCAGGCGTA+TGG 0.483216 5.3:-48063921 MS.gene035960:CDS
CGCAAGGCTTGTTGGCTATG+GGG 0.490281 5.3:-48062963 MS.gene035960:CDS
CTTAATTTGAGTGTTGAGTA+GGG 0.496496 5.3:+48062875 None:intergenic
CCGAAGATGCACTATCAATA+GGG 0.508074 5.3:-48063338 MS.gene035960:CDS
CTTTCAGATGGCTGATGCAA+TGG 0.508443 5.3:-48063247 MS.gene035960:CDS
CATAAGTAGCATGGAGAACA+AGG 0.509417 5.3:-48065136 MS.gene035960:intron
CACATAGTCACTTGAAGGAA+TGG 0.522645 5.3:+48065225 None:intergenic
CATTCCTTCAAGTGACTATG+TGG 0.523116 5.3:-48065224 MS.gene035960:CDS
ATAACAGAGTTTGTTGCCTA+AGG 0.529419 5.3:+48063019 None:intergenic
TTGAAATTGAATATGGACGT+TGG 0.536052 5.3:-48064095 MS.gene035960:CDS
AGAAGCAGTTTATTGGATGT+CGG 0.539030 5.3:-48064240 MS.gene035960:CDS
TTCGCACTCTTAGTTCATTG+TGG 0.540430 5.3:-48062924 MS.gene035960:CDS
TATTCAAGAACCTGTTCCTA+AGG 0.542554 5.3:-48064963 MS.gene035960:intron
CGGAAACTACGGCTTTCAGA+TGG 0.543160 5.3:-48063259 MS.gene035960:CDS
TAGATTGAAGCTCATGCAGT+TGG 0.543282 5.3:-48064404 MS.gene035960:CDS
AATGAAAGCAGAAGCTGCAA+AGG 0.554661 5.3:-48063953 MS.gene035960:CDS
AGATGCACTATCAATAGGGT+TGG 0.557521 5.3:-48063334 MS.gene035960:CDS
TCGATTGGTCAAGCTGCGCA+AGG 0.570513 5.3:-48062979 MS.gene035960:CDS
ATGTCACGCATCTTATCGAT+TGG 0.579770 5.3:-48062994 MS.gene035960:CDS
GCAAGGCTTGTTGGCTATGG+GGG 0.585303 5.3:-48062962 MS.gene035960:CDS
GCCGTAGTTTCCGAAATCCA+AGG 0.586415 5.3:+48063269 None:intergenic
GAAGATGAACATAAGTAGCA+TGG 0.592197 5.3:-48065145 MS.gene035960:CDS
TGTACATTTAGAATTTGTGA+TGG 0.598170 5.3:+48063853 None:intergenic
GCGCGGCTGCTCGAAAATGT+CGG 0.599361 5.3:-48064538 MS.gene035960:CDS
GTCTCTTGATCATCTCCAGA+AGG 0.603267 5.3:+48064986 None:intergenic
TCGTCTGGAACAATAAACTC+AGG 0.632327 5.3:-48064207 MS.gene035960:intron
CTCAGGCGTATGGAAATCAC+CGG 0.632473 5.3:-48063911 MS.gene035960:CDS
ATGGCTGATGCAATGGACAA+AGG 0.633905 5.3:-48063240 MS.gene035960:CDS
CCGTAGTTTCCGAAATCCAA+GGG 0.636532 5.3:+48063270 None:intergenic
CCTTCAAGTGACTATGTGGG+AGG 0.650193 5.3:-48065220 MS.gene035960:CDS
CTTGCTGAGATGATAAACGC+AGG 0.654339 5.3:+48063361 None:intergenic
ATTCCTTCAAGTGACTATGT+GGG 0.664208 5.3:-48065223 MS.gene035960:CDS
TATGTTCATCTTCAACATTG+GGG 0.698417 5.3:+48065155 None:intergenic
ACGTCAAGCACAAAATCGCG+CGG 0.713710 5.3:-48064555 MS.gene035960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAGATAATAGTAATTAG+TGG + chr5.3:48064335-48064354 None:intergenic 15.0%
!! AGTAGAAAAAATTGAAATAA+CGG - chr5.3:48063239-48063258 MS.gene035960:CDS 15.0%
!! ATAAGATAATAGTAATTAGT+GGG + chr5.3:48064334-48064353 None:intergenic 15.0%
!!! TAATTAATTTTTTTGACACA+AGG + chr5.3:48063507-48063526 None:intergenic 15.0%
!! GTTGTAAATACTGAATTTAT+AGG - chr5.3:48063658-48063677 MS.gene035960:intron 20.0%
!! TAAATACTGAATTTATAGGA+AGG - chr5.3:48063662-48063681 MS.gene035960:intron 20.0%
!! TATAGTCTATATTCGTTAAT+AGG - chr5.3:48064356-48064375 MS.gene035960:intron 20.0%
!! TATCAATATTTATCCTTGTA+GGG - chr5.3:48063891-48063910 MS.gene035960:CDS 20.0%
!! TATCTTGAAATAGTTCATAA+TGG - chr5.3:48063539-48063558 MS.gene035960:intron 20.0%
!! TTATCAATATTTATCCTTGT+AGG - chr5.3:48063890-48063909 MS.gene035960:CDS 20.0%
!! TTTATATCAAATAGAGAAAG+AGG + chr5.3:48063842-48063861 None:intergenic 20.0%
!!! ATAGTTTTAACATAGTATCA+TGG - chr5.3:48063467-48063486 MS.gene035960:intron 20.0%
!!! GAATTTCTATTTTGTTTCTA+TGG - chr5.3:48063063-48063082 MS.gene035960:intron 20.0%
!!! TCTTTGTTTGAAATTGAATA+TGG - chr5.3:48064059-48064078 MS.gene035960:CDS 20.0%
!!! TTTCTATTTTTTTGATTCAC+AGG - chr5.3:48063709-48063728 MS.gene035960:intron 20.0%
! ATCTGTAAGAAAAAGTCAAA+TGG + chr5.3:48064726-48064745 None:intergenic 25.0%
! GAAAAAATTAAAATCGATGC+AGG + chr5.3:48065070-48065089 None:intergenic 25.0%
! GTTTCTCAATATATACGTAT+AGG - chr5.3:48063576-48063595 MS.gene035960:intron 25.0%
! TCATTCAAATTCTCAAGTTT+CGG + chr5.3:48064756-48064775 None:intergenic 25.0%
! TCGTTAATAGGTCAAAATAA+AGG - chr5.3:48064368-48064387 MS.gene035960:intron 25.0%
! TTATGTTCATCTTCAACATT+GGG + chr5.3:48063010-48063029 None:intergenic 25.0%
! TTTATACAGAAGCAGTTTAT+TGG - chr5.3:48063914-48063933 MS.gene035960:CDS 25.0%
! TTTGGAAAAGATTTGAGTAT+TGG + chr5.3:48064190-48064209 None:intergenic 25.0%
!! GATGTTTTCTATTTAATCTC+AGG - chr5.3:48064233-48064252 MS.gene035960:CDS 25.0%
!! GTTTTGTAATTCAGGTAATT+TGG - chr5.3:48064958-48064977 MS.gene035960:intron 25.0%
!! TGTACATTTAGAATTTGTGA+TGG + chr5.3:48064311-48064330 None:intergenic 25.0%
!!! AAGCATGATGATTTTTTATG+CGG + chr5.3:48063818-48063837 None:intergenic 25.0%
AAAGACGAGAAAAATAACAC+TGG - chr5.3:48063270-48063289 MS.gene035960:CDS 30.0%
AATTTAAACTTCGTCTCTTG+CGG - chr5.3:48064505-48064524 MS.gene035960:intron 30.0%
ACGAATTTACAACTGTTTAG+AGG - chr5.3:48064481-48064500 MS.gene035960:intron 30.0%
CTTATGTTCATCTTCAACAT+TGG + chr5.3:48063011-48063030 None:intergenic 30.0%
GTGTATTAGAATTCTAACGT+GGG - chr5.3:48065036-48065055 MS.gene035960:intron 30.0%
TATCGAGAAAACAAGAAAAC+AGG - chr5.3:48063787-48063806 MS.gene035960:intron 30.0%
TATGTTCATCTTCAACATTG+GGG + chr5.3:48063009-48063028 None:intergenic 30.0%
TGATGTAATTGTATCACCTT+AGG + chr5.3:48063217-48063236 None:intergenic 30.0%
TGTGTATTAGAATTCTAACG+TGG - chr5.3:48065035-48065054 MS.gene035960:intron 30.0%
TTGAAATTGAATATGGACGT+TGG - chr5.3:48064066-48064085 MS.gene035960:CDS 30.0%
! TCATACATGTTCATCCTTTT+CGG + chr5.3:48062915-48062934 None:intergenic 30.0%
! TTATTGGATGTCGGTTATAT+GGG - chr5.3:48063930-48063949 MS.gene035960:CDS 30.0%
! TTTATTGGATGTCGGTTATA+TGG - chr5.3:48063929-48063948 MS.gene035960:CDS 30.0%
!! AGTAGAAGGTTTTGTAATTC+AGG - chr5.3:48064950-48064969 MS.gene035960:intron 30.0%
!!! TGCATTTTTTCCTTCTTTAC+AGG - chr5.3:48065112-48065131 MS.gene035960:intron 30.0%
AAGGTTCTCTTGATTCTTGA+GGG + chr5.3:48063159-48063178 None:intergenic 35.0%
AATTGTATCACCTTAGGAAC+AGG + chr5.3:48063211-48063230 None:intergenic 35.0%
AGAAGCAGTTTATTGGATGT+CGG - chr5.3:48063921-48063940 MS.gene035960:CDS 35.0%
ATCCTCCTATCCAAAGAAAA+AGG + chr5.3:48064283-48064302 None:intergenic 35.0%
ATTCCTTCAAGTGACTATGT+GGG - chr5.3:48062938-48062957 MS.gene035960:CDS 35.0%
GAAGATGAACATAAGTAGCA+TGG - chr5.3:48063016-48063035 MS.gene035960:CDS 35.0%
TATTAGAATTCTAACGTGGG+TGG - chr5.3:48065039-48065058 MS.gene035960:intron 35.0%
TATTCAAGAACCTGTTCCTA+AGG - chr5.3:48063198-48063217 MS.gene035960:intron 35.0%
TCTATTTAATCTCAGGCGTA+TGG - chr5.3:48064240-48064259 MS.gene035960:CDS 35.0%
! TGTCATTTCATGTAAACCTC+AGG - chr5.3:48064641-48064660 MS.gene035960:intron 35.0%
!! ATAACAGAGTTTGTTGCCTA+AGG + chr5.3:48065145-48065164 None:intergenic 35.0%
!! TGACTTTTTCTTACAGATCG+TGG - chr5.3:48064728-48064747 MS.gene035960:intron 35.0%
AATGAAAGCAGAAGCTGCAA+AGG - chr5.3:48064208-48064227 MS.gene035960:CDS 40.0%
ACAGGCTTATGTTCAACAAC+TGG - chr5.3:48063727-48063746 MS.gene035960:intron 40.0%
AGATGCACTATCAATAGGGT+TGG - chr5.3:48064827-48064846 MS.gene035960:intron 40.0%
ATCACACATAACATGTGTCG+TGG - chr5.3:48065003-48065022 MS.gene035960:CDS 40.0%
ATGTCACGCATCTTATCGAT+TGG - chr5.3:48065167-48065186 MS.gene035960:CDS 40.0%
CAAGAATCAAGAGAACCTTC+TGG - chr5.3:48063160-48063179 MS.gene035960:intron 40.0%
CACATAGTCACTTGAAGGAA+TGG + chr5.3:48062939-48062958 None:intergenic 40.0%
CATAAGTAGCATGGAGAACA+AGG - chr5.3:48063025-48063044 MS.gene035960:CDS 40.0%
CATTCCTTCAAGTGACTATG+TGG - chr5.3:48062937-48062956 MS.gene035960:CDS 40.0%
CCGAAGATGCACTATCAATA+GGG - chr5.3:48064823-48064842 MS.gene035960:intron 40.0%
GAAGGTTCTCTTGATTCTTG+AGG + chr5.3:48063160-48063179 None:intergenic 40.0%
GCTTCTGTATAAACCCTACA+AGG + chr5.3:48063907-48063926 None:intergenic 40.0%
TAGATTGAAGCTCATGCAGT+TGG - chr5.3:48063757-48063776 MS.gene035960:intron 40.0%
TCAGCTCAGTGGTAAAAGTA+TGG + chr5.3:48064539-48064558 None:intergenic 40.0%
TGTTGTTGATCATACTCTGC+TGG + chr5.3:48064860-48064879 None:intergenic 40.0%
TTCGCACTCTTAGTTCATTG+TGG - chr5.3:48065237-48065256 MS.gene035960:CDS 40.0%
TTGCAGCTTCTGCTTTCATT+TGG + chr5.3:48064208-48064227 None:intergenic 40.0%
! AAAATGTCGGCTGCGAAAAA+AGG - chr5.3:48063636-48063655 MS.gene035960:intron 40.0%
! CTTATGATTTGTCAGCTCAG+TGG + chr5.3:48064550-48064569 None:intergenic 40.0%
! GAACGCCTTTTTCTTTGGAT+AGG - chr5.3:48064275-48064294 MS.gene035960:intron 40.0%
! TCGTCTGGAACAATAAACTC+AGG - chr5.3:48063954-48063973 MS.gene035960:CDS 40.0%
!! CCCTATTGATAGTGCATCTT+CGG + chr5.3:48064826-48064845 None:intergenic 40.0%
AGGTGATCTGCCTGTAAAGA+AGG + chr5.3:48065125-48065144 None:intergenic 45.0%
ATGGCTGATGCAATGGACAA+AGG - chr5.3:48064921-48064940 MS.gene035960:intron 45.0%
CAACATTCAAGCTGATCCCT+TGG - chr5.3:48064875-48064894 MS.gene035960:intron 45.0%
CAAGCTGATCCCTTGGATTT+CGG - chr5.3:48064882-48064901 MS.gene035960:intron 45.0%
CCCTTGGATTTCGGAAACTA+CGG - chr5.3:48064891-48064910 MS.gene035960:intron 45.0%
CCGTAGTTTCCGAAATCCAA+GGG + chr5.3:48064894-48064913 None:intergenic 45.0%
CGCTATAGAAACATGTCCTG+AGG + chr5.3:48064660-48064679 None:intergenic 45.0%
CTTCGTCTCTTGCGGTTATA+TGG - chr5.3:48064513-48064532 MS.gene035960:intron 45.0%
CTTGCTGAGATGATAAACGC+AGG + chr5.3:48064803-48064822 None:intergenic 45.0%
CTTTACAGGCAGATCACCTT+AGG - chr5.3:48065126-48065145 MS.gene035960:intron 45.0%
CTTTCAGATGGCTGATGCAA+TGG - chr5.3:48064914-48064933 MS.gene035960:intron 45.0%
GAAGCTCATGCAGTTGGAAT+TGG - chr5.3:48063763-48063782 MS.gene035960:intron 45.0%
GACAAAGGAGAAGCAGTAGA+AGG - chr5.3:48064936-48064955 MS.gene035960:intron 45.0%
GCCGAAGATGCACTATCAAT+AGG - chr5.3:48064822-48064841 MS.gene035960:intron 45.0%
GTCTCTTGATCATCTCCAGA+AGG + chr5.3:48063178-48063197 None:intergenic 45.0%
TCCTGTCATGTTTCGAAGCA+GGG - chr5.3:48064033-48064052 MS.gene035960:CDS 45.0%
! CGCCTTTTTCTTTGGATAGG+AGG - chr5.3:48064278-48064297 MS.gene035960:intron 45.0%
!! CAAAGAAAAAGGCGTTCGAC+CGG + chr5.3:48064272-48064291 None:intergenic 45.0%
!!! TTTTAAAAAATATCATTTTA+AGG + chr5.3:48064010-48064029 None:intergenic 5.0%
AAGCGTGTGTTGTCTAGACG+AGG - chr5.3:48064567-48064586 MS.gene035960:intron 50.0%
ACGTCAAGCACAAAATCGCG+CGG - chr5.3:48063606-48063625 MS.gene035960:intron 50.0%
CCTCCCACATAGTCACTTGA+AGG + chr5.3:48062944-48062963 None:intergenic 50.0%
CCTTCAAGTGACTATGTGGG+AGG - chr5.3:48062941-48062960 MS.gene035960:CDS 50.0%
CGGAAACTACGGCTTTCAGA+TGG - chr5.3:48064902-48064921 MS.gene035960:intron 50.0%
CTCAACTAATCGCGCCGAAA+AGG - chr5.3:48062898-48062917 MS.gene035960:CDS 50.0%
CTCAGGCGTATGGAAATCAC+CGG - chr5.3:48064250-48064269 MS.gene035960:CDS 50.0%
GCCGTAGTTTCCGAAATCCA+AGG + chr5.3:48064895-48064914 None:intergenic 50.0%
GTCCTGTCATGTTTCGAAGC+AGG - chr5.3:48064032-48064051 MS.gene035960:CDS 50.0%
GTCGGTTATATGGGATCGTC+TGG - chr5.3:48063939-48063958 MS.gene035960:CDS 50.0%
TCCCTGCTTCGAAACATGAC+AGG + chr5.3:48064037-48064056 None:intergenic 50.0%
! CGGTCGAACGCCTTTTTCTT+TGG - chr5.3:48064270-48064289 MS.gene035960:intron 50.0%
TCAAGCTGCGCAAGGCTTGT+TGG - chr5.3:48065190-48065209 MS.gene035960:CDS 55.0%
! TCGATTGGTCAAGCTGCGCA+AGG - chr5.3:48065182-48065201 MS.gene035960:CDS 55.0%
!! CGCAAGGCTTGTTGGCTATG+GGG - chr5.3:48065198-48065217 MS.gene035960:CDS 55.0%
!! GCAAGGCTTGTTGGCTATGG+GGG - chr5.3:48065199-48065218 MS.gene035960:CDS 55.0%
!! GCGCAAGGCTTGTTGGCTAT+GGG - chr5.3:48065197-48065216 MS.gene035960:CDS 55.0%
!! TGCGCAAGGCTTGTTGGCTA+TGG - chr5.3:48065196-48065215 MS.gene035960:CDS 55.0%
GCGCGGCTGCTCGAAAATGT+CGG - chr5.3:48063623-48063642 MS.gene035960:intron 60.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 48062876 48065307 48062876 ID=MS.gene035960
chr5.3 mRNA 48062876 48065307 48062876 ID=MS.gene035960.t1;Parent=MS.gene035960
chr5.3 exon 48065137 48065307 48065137 ID=MS.gene035960.t1.exon1;Parent=MS.gene035960.t1
chr5.3 CDS 48065137 48065307 48065137 ID=cds.MS.gene035960.t1;Parent=MS.gene035960.t1
chr5.3 exon 48064964 48065038 48064964 ID=MS.gene035960.t1.exon2;Parent=MS.gene035960.t1
chr5.3 CDS 48064964 48065038 48064964 ID=cds.MS.gene035960.t1;Parent=MS.gene035960.t1
chr5.3 exon 48064526 48064585 48064526 ID=MS.gene035960.t1.exon3;Parent=MS.gene035960.t1
chr5.3 CDS 48064526 48064585 48064526 ID=cds.MS.gene035960.t1;Parent=MS.gene035960.t1
chr5.3 exon 48064375 48064452 48064375 ID=MS.gene035960.t1.exon4;Parent=MS.gene035960.t1
chr5.3 CDS 48064375 48064452 48064375 ID=cds.MS.gene035960.t1;Parent=MS.gene035960.t1
chr5.3 exon 48064208 48064271 48064208 ID=MS.gene035960.t1.exon5;Parent=MS.gene035960.t1
chr5.3 CDS 48064208 48064271 48064208 ID=cds.MS.gene035960.t1;Parent=MS.gene035960.t1
chr5.3 exon 48063858 48064129 48063858 ID=MS.gene035960.t1.exon6;Parent=MS.gene035960.t1
chr5.3 CDS 48063858 48064129 48063858 ID=cds.MS.gene035960.t1;Parent=MS.gene035960.t1
chr5.3 exon 48063212 48063439 48063212 ID=MS.gene035960.t1.exon7;Parent=MS.gene035960.t1
chr5.3 CDS 48063212 48063439 48063212 ID=cds.MS.gene035960.t1;Parent=MS.gene035960.t1
chr5.3 exon 48062876 48063049 48062876 ID=MS.gene035960.t1.exon8;Parent=MS.gene035960.t1
chr5.3 CDS 48062876 48063049 48062876 ID=cds.MS.gene035960.t1;Parent=MS.gene035960.t1
Gene Sequence

>MS.gene035960

ATGAACTCTTCATCATCATCAACTCAACTAATCGCGCCGAAAAGGATGAACATGTATGAGCCATTCCTTCAAGTGACTATGTGGGAGGATAGCTTTAAAGTTGATCATAGTACTTTGAATTCAATTGCTTCCCCAATGTTGAAGATGAACATAAGTAGCATGGAGAACAAGGTGAAATTGAACTCATGAATTTCTATTTTGTTTCTATGGTTATCTATGTTTTATGAGAAAACTAGTTGACAATAGTCTTTTGAATTTTATTTGTATAGTCTGAATGTATCCCTCAAGAATCAAGAGAACCTTCTGGAGATGATCAAGAGACTATTCAAGAACCTGTTCCTAAGGTGATACAATTACATCACTAGTAGAAAAAATTGAAATAACGGCAGAAATTAAAGACGAGAAAAATAACACTGGTTACTAAATTTTGAGTTTCTAACACTTAGAGACGCTTCTTCAAAAAAAAAAACACTTAGAGACGCATTTTGTCTTTTTTTGTCTCTAAATTTTTGTCGTTATTTCAACTTTTTTAAGTAGTGATTTATATTTCTTTTATATATTTAGTCATGATAATTTTCATGTTTCATTTTAATAGTTTTAACATAGTATCATGGAGTTTTAGAAGTGACCTTGTGTCAAAAAAATTAATTAAAGAGAATTGTTTATCTTGAAATAGTTCATAATGGATTCTGACTTTATAGTTTCTCAATATATACGTATAGGTACTACGACGTCAAGCACAAAATCGCGCGGCTGCTCGAAAATGTCGGCTGCGAAAAAAGGTTGTAAATACTGAATTTATAGGAAGGAAGTTACTAGTGTTTATGATCTAATTTCTATTTTTTTGATTCACAGGCTTATGTTCAACAACTGGAAACAAGTAGATTGAAGCTCATGCAGTTGGAATTGGATATCGAGAAAACAAGAAAACAGGTGATACCGCATAAAAAATCATCATGCTTACCTCTTTCTCTATTTGATATAAATTATTTCATCTATCATTCATAAAAAGATTTATCAATATTTATCCTTGTAGGGTTTATACAGAAGCAGTTTATTGGATGTCGGTTATATGGGATCGTCTGGAACAATAAACTCAGGTTAGTGATTCATAATTGTAGTTTTAAGTTTCCTTAAAATGATATTTTTTAAAATAGTCCTGTCATGTTTCGAAGCAGGGATATCTTTGTTTGAAATTGAATATGGACGTTGGATTGAAGAACAAGATCGACAAAACGAAGAACTAAGAAATGCATTGCAAACTAATGCATCTGATATACAACTTCATCTACTTGTTGAGAGTAGCTTGAACCAATACTCAAATCTTTTCCAAATGAAAGCAGAAGCTGCAAAGGCTGATGTTTTCTATTTAATCTCAGGCGTATGGAAATCACCGGTCGAACGCCTTTTTCTTTGGATAGGAGGATCTCGTCCATCACAAATTCTAAATGTACACCCACTAATTACTATTATCTTATTTTATAGTCTATATTCGTTAATAGGTCAAAATAAAGGAGCTTTATATAATCTTATTTGTTGAAATTTTGTTAATATATTCTTGATCAGTAGTTTATAGAAACTAGAATCTGCTTATAATTACTATTGACGAATTTACAACTGTTTAGAGGAAATTTAAACTTCGTCTCTTGCGGTTATATGGCCATACTTTTACCACTGAGCTGACAAATCATAAGCGTGTGTTGTCTAGACGAGGAGTACTAATTGCAAAAAACTTGTTCTATGTTTTGTAAATTGTTAAACATAATGTCATTTCATGTAAACCTCAGGACATGTTTCTATAGCGTCGATTGATACATGTAGCTAATCTATCAATAATTTTGTACAAACCATTTGACTTTTTCTTACAGATCGTGGTACCGAAACTTGAGAATTTGAATGATCAACAAATTGCTAGCATTAACAGCCTGCGTTTATCATCTCAGCAAGCCGAAGATGCACTATCAATAGGGTTGGAGAAACTCCAGCAGAGTATGATCAACAACATTCAAGCTGATCCCTTGGATTTCGGAAACTACGGCTTTCAGATGGCTGATGCAATGGACAAAGGAGAAGCAGTAGAAGGTTTTGTAATTCAGGTAATTTGGTCTTGTTTGTTGTTTGTTCTCAATCACACATAACATGTGTCGTGGAAACGTTAATGTGTATTAGAATTCTAACGTGGGTGGTTAGTCCTGCATCGATTTTAATTTTTTCGCACTTTCTATAGCTAAATTGATGCATTTTTTCCTTCTTTACAGGCAGATCACCTTAGGCAACAAACTCTGTTATACATGTCACGCATCTTATCGATTGGTCAAGCTGCGCAAGGCTTGTTGGCTATGGGGGAATACTTTCATCGTCTTCGCACTCTTAGTTCATTGTGGACTGCTCGTTCATGTCATCATTTCTACCCTACTCAACACTCAAATTAA
Protein sequence

>MS.gene035960.t1

MNSSSSSTQLIAPKRMNMYEPFLQVTMWEDSFKVDHSTLNSIASPMLKMNISSMENKSECIPQESREPSGDDQETIQEPVPKVLRRQAQNRAAARKCRLRKKAYVQQLETSRLKLMQLELDIEKTRKQGLYRSSLLDVGYMGSSGTINSGISLFEIEYGRWIEEQDRQNEELRNALQTNASDIQLHLLVESSLNQYSNLFQMKAEAAKADVFYLISGVWKSPVERLFLWIGGSRPSQILNIVVPKLENLNDQQIASINSLRLSSQQAEDALSIGLEKLQQSMINNIQADPLDFGNYGFQMADAMDKGEAVEGFVIQADHLRQQTLLYMSRILSIGQAAQGLLAMGEYFHRLRTLSSLWTARSCHHFYPTQHSN