AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035972

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035972.t1 MTR_5g061890 90.237 379 29 2 1 376 1 374 0.0 695
MS.gene035972.t1 MTR_5g060780 88.918 379 34 2 1 376 1 374 0.0 682
MS.gene035972.t1 MTR_5g460920 88.918 379 34 2 1 376 1 374 0.0 679
MS.gene035972.t1 MTR_5g060630 54.676 417 136 8 1 367 1 414 1.25e-132 390
MS.gene035972.t1 MTR_5g460740 54.676 417 136 8 1 367 1 414 1.25e-132 390
MS.gene035972.t1 MTR_5g061220 53.165 395 144 9 1 364 8 392 5.44e-126 373
MS.gene035972.t1 MTR_1g058210 53.018 381 143 10 1 364 1 362 4.26e-117 348
MS.gene035972.t1 MTR_2g024430 52.381 357 136 16 1 341 9 347 2.38e-103 315
MS.gene035972.t1 MTR_5g040880 49.558 339 146 9 8 335 7 331 8.87e-96 295
MS.gene035972.t1 MTR_2g006270 45.312 384 179 14 6 374 7 374 5.61e-90 278
MS.gene035972.t1 MTR_5g040740 50.904 332 128 11 1 315 1 314 1.54e-87 271
MS.gene035972.t1 MTR_2g006380 42.520 381 187 14 6 374 14 374 2.10e-86 269
MS.gene035972.t1 MTR_5g082140 45.376 346 160 10 6 336 6 337 7.65e-85 265
MS.gene035972.t1 MTR_5g060770 51.803 305 91 7 63 365 9 259 8.02e-80 249
MS.gene035972.t1 MTR_3g073420 34.048 420 199 12 9 378 16 407 2.35e-62 210
MS.gene035972.t1 MTR_5g062970 56.122 196 70 7 12 195 38 229 1.47e-61 198
MS.gene035972.t1 MTR_5g460910 45.741 317 93 10 52 365 14 254 3.95e-61 200
MS.gene035972.t1 MTR_2g094570 35.356 379 184 12 9 336 8 376 7.92e-57 197
MS.gene035972.t1 MTR_1g094960 35.294 408 192 16 9 361 16 406 9.32e-57 196
MS.gene035972.t1 MTR_2g094570 35.356 379 184 12 9 336 8 376 1.05e-56 197
MS.gene035972.t1 MTR_2g094570 35.356 379 184 12 9 336 64 432 1.79e-56 197
MS.gene035972.t1 MTR_1g064430 36.496 411 190 15 9 360 24 422 1.86e-56 196
MS.gene035972.t1 MTR_1g064430 36.496 411 190 15 9 360 37 435 2.34e-56 196
MS.gene035972.t1 MTR_4g058930 36.154 390 181 13 9 340 9 388 1.19e-54 191
MS.gene035972.t1 MTR_4g058930 36.154 390 181 13 9 340 9 388 1.74e-54 191
MS.gene035972.t1 MTR_7g101280 30.273 403 194 13 9 341 28 413 1.14e-46 169
MS.gene035972.t1 MTR_0041s0110 30.273 403 194 13 9 341 28 413 1.14e-46 169
MS.gene035972.t1 MTR_2g014770 29.897 388 228 15 4 364 43 413 1.97e-40 151
MS.gene035972.t1 MTR_2g014770 29.897 388 228 15 4 364 43 413 3.17e-40 151
MS.gene035972.t1 MTR_5g062960 63.393 112 40 1 227 338 1 111 9.99e-40 140
MS.gene035972.t1 MTR_2g005240 29.199 387 227 12 12 371 23 389 4.12e-38 145
MS.gene035972.t1 MTR_7g101275 32.063 315 176 11 22 314 2 300 1.07e-37 140
MS.gene035972.t1 MTR_4g021580 28.889 360 234 9 12 358 19 369 1.25e-37 143
MS.gene035972.t1 MTR_4g021580 28.889 360 234 9 12 358 19 369 1.93e-37 143
MS.gene035972.t1 MTR_7g062540 28.809 361 236 9 12 359 14 366 1.94e-36 140
MS.gene035972.t1 MTR_8g100050 28.448 348 210 9 12 335 59 391 5.33e-36 139
MS.gene035972.t1 MTR_8g100050 28.448 348 210 9 12 335 59 391 1.04e-35 139
MS.gene035972.t1 MTR_8g027440 29.521 376 241 12 12 373 29 394 1.92e-34 134
MS.gene035972.t1 MTR_4g088210 28.497 386 229 15 12 369 38 404 3.91e-34 134
MS.gene035972.t1 MTR_4g088210 28.497 386 229 15 12 369 38 404 4.04e-34 134
MS.gene035972.t1 MTR_2g018690 28.863 343 223 9 9 335 20 357 4.38e-34 134
MS.gene035972.t1 MTR_0041s0120 30.864 324 180 11 22 314 2 312 1.53e-33 129
MS.gene035972.t1 MTR_5g076270 28.824 340 221 9 12 335 24 358 1.14e-31 127
MS.gene035972.t1 MTR_8g079492 27.429 350 218 10 9 335 20 356 1.55e-31 126
MS.gene035972.t1 MTR_8g079492 27.429 350 218 10 9 335 20 356 1.70e-31 126
MS.gene035972.t1 MTR_4g124900 27.697 343 225 9 9 335 20 355 1.80e-31 126
MS.gene035972.t1 MTR_4g124900 27.697 343 225 9 9 335 20 355 1.85e-31 126
MS.gene035972.t1 MTR_2g093740 29.723 397 233 16 12 376 56 438 4.80e-31 125
MS.gene035972.t1 MTR_2g093740 29.723 397 233 16 12 376 56 438 6.32e-31 124
MS.gene035972.t1 MTR_2g093740 29.723 397 233 16 12 376 56 438 7.47e-31 124
MS.gene035972.t1 MTR_2g043250 29.651 344 217 13 9 335 20 355 1.14e-30 124
MS.gene035972.t1 MTR_3g064050 28.529 340 222 9 12 335 26 360 2.22e-30 123
MS.gene035972.t1 MTR_1g024025 29.619 341 216 11 12 335 43 376 6.51e-30 122
MS.gene035972.t1 MTR_4g060460 28.861 395 238 15 12 376 54 435 1.97e-29 120
MS.gene035972.t1 MTR_2g093740 29.268 410 231 17 12 376 56 451 4.80e-29 119
MS.gene035972.t1 MTR_2g093740 29.268 410 231 17 12 376 56 451 5.64e-29 119
MS.gene035972.t1 MTR_2g093740 29.268 410 231 17 12 376 56 451 8.15e-29 118
MS.gene035972.t1 MTR_4g060460 28.718 390 235 15 12 371 54 430 2.14e-28 117
MS.gene035972.t1 MTR_8g101360 28.693 352 212 11 9 335 28 365 2.46e-26 111
MS.gene035972.t1 MTR_8g446900 27.181 298 173 12 61 335 12 288 5.00e-20 90.9
MS.gene035972.t1 MTR_8g079492 26.829 287 176 9 70 335 1 274 1.83e-19 90.9
MS.gene035972.t1 MTR_2g093740 28.470 281 175 11 112 376 38 308 2.15e-15 78.2
MS.gene035972.t1 MTR_5g046120 35.897 117 69 3 50 160 12 128 2.60e-15 72.4
MS.gene035972.t1 MTR_2g093740 28.470 281 175 11 112 376 38 308 2.71e-15 77.8
MS.gene035972.t1 MTR_2g093740 27.891 294 173 12 112 376 38 321 1.85e-13 72.0
MS.gene035972.t1 MTR_2g093740 27.891 294 173 12 112 376 38 321 2.42e-13 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035972.t1 AT1G77850 35.294 374 187 14 8 336 15 378 9.83e-62 208
MS.gene035972.t1 AT1G77850 35.294 374 187 14 8 336 15 378 1.01e-61 208
MS.gene035972.t1 AT4G30080 35.806 391 190 14 9 347 16 397 6.96e-54 189
MS.gene035972.t1 AT2G28350 33.846 390 191 13 9 340 7 387 1.99e-51 182
MS.gene035972.t1 AT2G28350 33.846 390 191 13 9 340 7 387 2.82e-51 182
MS.gene035972.t1 AT2G46530 30.245 367 228 9 12 361 21 376 4.39e-43 158
MS.gene035972.t1 AT2G46530 30.245 367 228 9 12 361 42 397 5.53e-43 158
MS.gene035972.t1 AT3G61830 30.347 346 217 9 12 342 25 361 6.64e-42 155
MS.gene035972.t1 AT1G59750 31.675 382 230 13 12 374 22 391 1.69e-39 149
MS.gene035972.t1 AT1G59750 31.967 366 218 13 12 358 22 375 3.22e-39 148
MS.gene035972.t1 AT1G59750 31.967 366 218 13 12 358 22 375 3.22e-39 148
MS.gene035972.t1 AT1G59750 31.967 366 218 13 12 358 22 375 3.44e-39 148
MS.gene035972.t1 AT1G34390 30.295 373 221 12 12 364 27 380 7.03e-39 147
MS.gene035972.t1 AT1G35520 31.070 383 207 15 12 364 27 382 6.76e-38 144
MS.gene035972.t1 AT1G34410 30.184 381 213 15 12 364 27 382 1.21e-36 140
MS.gene035972.t1 AT1G34310 30.729 384 213 15 9 364 24 382 3.29e-36 139
MS.gene035972.t1 AT5G62000 28.818 347 210 10 12 335 61 393 7.91e-36 139
MS.gene035972.t1 AT5G62000 28.818 347 210 10 12 335 61 393 8.11e-36 139
MS.gene035972.t1 AT5G62000 28.818 347 210 10 12 335 61 393 8.11e-36 139
MS.gene035972.t1 AT5G62000 28.818 347 210 10 12 335 61 393 8.11e-36 139
MS.gene035972.t1 AT5G62000 28.818 347 210 10 12 335 61 393 8.11e-36 139
MS.gene035972.t1 AT1G35540 30.628 382 210 17 12 364 27 382 1.96e-35 137
MS.gene035972.t1 AT4G23980 28.530 347 223 9 12 340 12 351 2.03e-35 137
MS.gene035972.t1 AT4G23980 28.530 347 223 9 12 340 12 351 2.19e-35 137
MS.gene035972.t1 AT1G35240 30.579 363 196 15 12 343 27 364 3.14e-34 133
MS.gene035972.t1 AT1G30330 29.412 340 219 9 12 335 25 359 4.24e-34 134
MS.gene035972.t1 AT1G30330 29.412 340 219 9 12 335 25 359 4.24e-34 134
MS.gene035972.t1 AT1G30330 29.412 340 219 9 12 335 23 357 4.32e-34 134
MS.gene035972.t1 AT5G37020 29.912 341 216 11 12 335 22 356 1.78e-33 132
MS.gene035972.t1 AT5G37020 29.912 341 216 11 12 335 22 356 2.41e-33 132
MS.gene035972.t1 AT2G33860 29.049 389 229 13 12 378 54 417 4.10e-33 130
MS.gene035972.t1 AT1G34170 30.000 360 211 14 12 349 25 365 9.75e-33 128
MS.gene035972.t1 AT1G19220 28.530 347 217 11 9 335 20 355 1.99e-30 123
MS.gene035972.t1 AT5G20730 28.736 348 216 11 9 335 21 357 1.71e-29 120
MS.gene035972.t1 AT5G20730 28.736 348 216 11 9 335 21 357 1.92e-29 120
MS.gene035972.t1 AT1G19850 30.145 345 220 10 7 335 49 388 2.14e-29 120
MS.gene035972.t1 AT1G19850 30.145 345 220 10 7 335 38 377 2.34e-29 120
MS.gene035972.t1 AT3G61830 30.169 295 187 7 59 342 24 310 2.81e-29 119
MS.gene035972.t1 AT5G20730 28.242 347 218 10 9 335 21 356 4.64e-29 119
MS.gene035972.t1 AT5G60450 29.060 351 212 13 12 335 41 381 7.00e-29 119
MS.gene035972.t1 AT5G60450 29.060 351 212 13 12 335 65 405 7.71e-29 119
MS.gene035972.t1 AT1G43950 32.353 204 118 6 12 203 27 222 1.94e-26 105
MS.gene035972.t1 AT1G34170 27.731 357 197 13 12 349 25 339 9.43e-26 108
MS.gene035972.t1 AT1G34170 27.731 357 197 13 12 349 25 339 1.35e-25 108
MS.gene035972.t1 AT2G46530 29.389 262 166 7 111 361 36 289 6.61e-23 100
MS.gene035972.t1 AT2G33860 28.674 279 167 10 112 378 2 260 1.57e-19 90.1
MS.gene035972.t1 AT5G37020 29.958 237 147 9 112 335 31 261 4.21e-17 83.6

Find 99 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGCATATCTGCCATATTT+TGG 0.143600 5.3:-47779489 None:intergenic
AACTGATGAGATTAAGGTTT+TGG 0.252605 5.3:+47780117 MS.gene035972:CDS
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5.3:-47779530 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5.3:+47780365 MS.gene035972:CDS
TGTCTCCAACATCGTCTTCT+TGG 0.287447 5.3:-47779757 None:intergenic
GATGACAATTTCTCAGCTTA+AGG 0.289847 5.3:+47780318 MS.gene035972:CDS
GAATTTAACTGATGAGATTA+AGG 0.298587 5.3:+47780111 MS.gene035972:CDS
CGACCCTGCCAAGGTTTGTT+GGG 0.312042 5.3:-47780998 None:intergenic
ACCTCAAGCATGCGCCAATT+AGG 0.318991 5.3:-47780379 None:intergenic
TGCCAAGGATTGACTACGTT+TGG 0.321933 5.3:-47780880 None:intergenic
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5.3:+47780247 MS.gene035972:CDS
CTACTTCCCACGAGGTCATC+TGG 0.355762 5.3:+47779553 MS.gene035972:CDS
CCTCAAGCATGCGCCAATTA+GGG 0.367711 5.3:-47780378 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.375178 5.3:-47779515 None:intergenic
GATCTTTCCCAATCTTGCTT+TGG 0.377536 5.3:+47779859 MS.gene035972:CDS
AGATTTCCAACCAATCTTCA+TGG 0.383662 5.3:-47780245 None:intergenic
TCCAACATCGTCTTCTTGGC+CGG 0.392440 5.3:-47779753 None:intergenic
ATGACAATTTCTCAGCTTAA+GGG 0.392754 5.3:+47780319 MS.gene035972:CDS
TCGACCCTGCCAAGGTTTGT+TGG 0.395481 5.3:-47780999 None:intergenic
AGACTCGTCCAAAGCAAGAT+TGG 0.400052 5.3:-47779867 None:intergenic
CTCGTGTGGAACCCAAAATA+TGG 0.402726 5.3:+47779477 MS.gene035972:CDS
CTTGGTCAGTTACTGGAGTC+AGG 0.412247 5.3:-47779709 None:intergenic
GCCGGGAACTACAGGAGGAG+GGG 0.417463 5.3:-47779735 None:intergenic
CGCTTCATCTGTAGTGAACT+TGG 0.424216 5.3:-47780074 None:intergenic
TGGCCGGGAACTACAGGAGG+AGG 0.428083 5.3:-47779737 None:intergenic
CGACCTTAAAAGCCGAGAAT+GGG 0.441124 5.3:+47779907 MS.gene035972:CDS
TGGAATCTTGACGTCGGGGC+CGG 0.443805 5.3:-47779510 None:intergenic
CCAAGGTTTGGCTATGTGTC+AGG 0.447799 5.3:-47781033 None:intergenic
TCACCCCAACAAACCTTGGC+AGG 0.450090 5.3:+47780994 MS.gene035972:CDS
TCTTCTTGGCCGGGAACTAC+AGG 0.462528 5.3:-47779743 None:intergenic
CCGGGAACTACAGGAGGAGG+GGG 0.475602 5.3:-47779734 None:intergenic
GTTGACGATGCCATGAAGAT+TGG 0.477758 5.3:+47780235 MS.gene035972:CDS
TACTGGAGTCAGGAGCAGTT+TGG 0.478974 5.3:-47779699 None:intergenic
GACTCGTCCAAAGCAAGATT+GGG 0.479990 5.3:-47779866 None:intergenic
GGAAGAGACGATGCATAGAA+TGG 0.483840 5.3:-47779636 None:intergenic
GATGAAAACAACAGAATCGT+TGG 0.490573 5.3:-47780008 None:intergenic
GAAGATTCATATTTCTTCAA+AGG 0.492904 5.3:-47780286 None:intergenic
ATGGTTCTTGTGGCAGCAGT+AGG 0.493323 5.3:-47779593 None:intergenic
TTATCATAGGTTAACTGGGA+TGG 0.493859 5.3:+47780838 MS.gene035972:intron
CCGACCTTAAAAGCCGAGAA+TGG 0.494002 5.3:+47779906 MS.gene035972:CDS
GGCGGAGTTGACGGTGGAGG+AGG 0.496620 5.3:-47780938 None:intergenic
ATCCAAACGTAGTCAATCCT+TGG 0.506933 5.3:+47780878 MS.gene035972:CDS
TGAGGCGGAGTTGACGGTGG+AGG 0.507469 5.3:-47780941 None:intergenic
TCATCCTCTGCCACCTCGTG+TGG 0.517268 5.3:+47779463 MS.gene035972:CDS
TTGACTACGTTTGGATTCTG+TGG 0.517390 5.3:-47780871 None:intergenic
CCAACATCGTCTTCTTGGCC+GGG 0.517848 5.3:-47779752 None:intergenic
TGACTACGTTTGGATTCTGT+GGG 0.518626 5.3:-47780870 None:intergenic
AATCTCAGTAGATTACATAC+TGG 0.519388 5.3:+47779950 MS.gene035972:CDS
CCCTAATTGGCGCATGCTTG+AGG 0.522896 5.3:+47780378 MS.gene035972:CDS
GGTTCCACACGAGGTGGCAG+AGG 0.527487 5.3:-47779467 None:intergenic
TGGTTCTTGTGGCAGCAGTA+GGG 0.533159 5.3:-47779592 None:intergenic
TTCATCACCCCAACAAACCT+TGG 0.536178 5.3:+47780990 MS.gene035972:CDS
GATGAACTCTCAGCAAGTCT+AGG 0.537540 5.3:-47780973 None:intergenic
AGATATCTGTCGGAATTCGC+AGG 0.543135 5.3:+47780047 MS.gene035972:CDS
ACTACAGATGAAGCGGATGA+AGG 0.543135 5.3:+47780082 MS.gene035972:CDS
TTTGGATGCAGTGGAATTGG+CGG 0.544683 5.3:+47780135 MS.gene035972:CDS
TTGTAAACTTCAACCTGCCA+AGG 0.544687 5.3:-47780895 None:intergenic
AAACGTAGTCAATCCTTGGC+AGG 0.548583 5.3:+47780882 MS.gene035972:CDS
GAGACGATGCATAGAATGGA+TGG 0.556436 5.3:-47779632 None:intergenic
GAGGAGGAAGTCGAAGGTGC+CGG 0.556628 5.3:-47780922 None:intergenic
ACGATGCCATGAAGATTGGT+TGG 0.558168 5.3:+47780239 MS.gene035972:CDS
TCAGTAGATTACATACTGGC+TGG 0.560711 5.3:+47779954 MS.gene035972:CDS
GGTTCTTGTGGCAGCAGTAG+GGG 0.568653 5.3:-47779591 None:intergenic
GACCCTGCCAAGGTTTGTTG+GGG 0.572005 5.3:-47780997 None:intergenic
GGCCGGGAACTACAGGAGGA+GGG 0.579671 5.3:-47779736 None:intergenic
GGTTGAAGTTTACAACATAC+CGG 0.579800 5.3:+47780903 MS.gene035972:CDS
TGGACGGTAACGATCAAGAA+TGG 0.583957 5.3:-47779612 None:intergenic
CAAGTTCACTACAGATGAAG+CGG 0.584809 5.3:+47780075 MS.gene035972:CDS
AAAATATGGCAGATATGCGC+CGG 0.589425 5.3:+47779491 MS.gene035972:CDS
CCCGGCCAAGAAGACGATGT+TGG 0.598087 5.3:+47779752 MS.gene035972:CDS
TCTGCAGGAAAGATATCTGT+CGG 0.599009 5.3:+47780037 MS.gene035972:CDS
TGACAATTTCTCAGCTTAAG+GGG 0.599563 5.3:+47780320 MS.gene035972:CDS
TAATCTACTGAGATTTGCAT+AGG 0.604127 5.3:-47779942 None:intergenic
TGTTGATCCTCACACCGATG+AGG 0.613332 5.3:+47779670 MS.gene035972:CDS
CAAACCTTGGCAGGGTCGAG+TGG 0.615213 5.3:+47781003 MS.gene035972:CDS
GACCTTAAAAGCCGAGAATG+GGG 0.616896 5.3:+47779908 MS.gene035972:CDS
ACAGATGAAGCGGATGAAGG+AGG 0.630115 5.3:+47780085 MS.gene035972:CDS
AATCTTTGGAATCTTGACGT+CGG 0.630358 5.3:-47779516 None:intergenic
ACGATCAAGAATGGTTCTTG+TGG 0.631537 5.3:-47779603 None:intergenic
TCTTGAGGCGGAGTTGACGG+TGG 0.632846 5.3:-47780944 None:intergenic
GTGTACTATCCGACAGCTAG+TGG 0.633284 5.3:+47780181 MS.gene035972:CDS
ACTATCCGACAGCTAGTGGT+TGG 0.635927 5.3:+47780185 MS.gene035972:CDS
TTGTGTGAGAGTCTTGACAT+AGG 0.637006 5.3:-47779789 None:intergenic
AGTACCACTCGACCCTGCCA+AGG 0.642496 5.3:-47781007 None:intergenic
CACCCCAACAAACCTTGGCA+GGG 0.644050 5.3:+47780995 MS.gene035972:CDS
CAGTTTGGTAAAGACCTCAT+CGG 0.647300 5.3:-47779684 None:intergenic
AGGATCAACAAAGAGATCAA+CGG 0.651533 5.3:-47779657 None:intergenic
TCACGCCAACCACTAGCTGT+CGG 0.652491 5.3:-47780190 None:intergenic
ACCTTAAAAGCCGAGAATGG+GGG 0.652954 5.3:+47779909 MS.gene035972:CDS
TCTTGGCCGGGAACTACAGG+AGG 0.657068 5.3:-47779740 None:intergenic
CGATGCATAGAATGGATGGA+CGG 0.657644 5.3:-47779628 None:intergenic
AGGTGGCAGAGGATGAGACA+TGG 0.663852 5.3:-47779456 None:intergenic
TCTTTGGAATCTTGACGTCG+GGG 0.665271 5.3:-47779514 None:intergenic
GTAAAGACCTCATCGGTGTG+AGG 0.670012 5.3:-47779677 None:intergenic
ATCGAGTTAGAGCATTCGAT+AGG 0.670484 5.3:-47779839 None:intergenic
CATGTTCCAGATGACCTCGT+GGG 0.672658 5.3:-47779559 None:intergenic
GCATGTTCCAGATGACCTCG+TGG 0.672744 5.3:-47779560 None:intergenic
ACGGTGGAGGAGGAAGTCGA+AGG 0.704506 5.3:-47780928 None:intergenic
AAAGTTTACTACTTCCCACG+AGG 0.761653 5.3:+47779545 MS.gene035972:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTATCTTTTTATCAT+AGG + chr5.3:47780825-47780844 MS.gene035972:intron 15.0%
!! TCAATCAAATAATCTTATCA+TGG - chr5.3:47780458-47780477 None:intergenic 20.0%
! AAAAGTTTCTACTTAGCATT+AGG - chr5.3:47780736-47780755 None:intergenic 25.0%
! GAAGATTCATATTTCTTCAA+AGG - chr5.3:47780289-47780308 None:intergenic 25.0%
! GAATTTAACTGATGAGATTA+AGG + chr5.3:47780111-47780130 MS.gene035972:CDS 25.0%
! GTACATATGCTTTCTTAATT+TGG + chr5.3:47780400-47780419 MS.gene035972:intron 25.0%
!! CTTTTTATCATAGGTTAACT+GGG + chr5.3:47780834-47780853 MS.gene035972:intron 25.0%
!! TCTTTTTATCATAGGTTAAC+TGG + chr5.3:47780833-47780852 MS.gene035972:intron 25.0%
!!! CTATTTTTAGTGAATATCAC+TGG - chr5.3:47780668-47780687 None:intergenic 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG - chr5.3:47779533-47779552 None:intergenic 25.0%
AATCTCAGTAGATTACATAC+TGG + chr5.3:47779950-47779969 MS.gene035972:CDS 30.0%
ATGACAATTTCTCAGCTTAA+GGG + chr5.3:47780319-47780338 MS.gene035972:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG + chr5.3:47780365-47780384 MS.gene035972:CDS 30.0%
TAATCTACTGAGATTTGCAT+AGG - chr5.3:47779945-47779964 None:intergenic 30.0%
! AACTGATGAGATTAAGGTTT+TGG + chr5.3:47780117-47780136 MS.gene035972:CDS 30.0%
!! AGGAGTATTTACAATGAGAA+AGG - chr5.3:47780716-47780735 None:intergenic 30.0%
AGATTTCCAACCAATCTTCA+TGG - chr5.3:47780248-47780267 None:intergenic 35.0%
AGGATCAACAAAGAGATCAA+CGG - chr5.3:47779660-47779679 None:intergenic 35.0%
CTATCAAGTGCAAGAACAAT+GGG - chr5.3:47780587-47780606 None:intergenic 35.0%
GATGAAAACAACAGAATCGT+TGG - chr5.3:47780011-47780030 None:intergenic 35.0%
GATGACAATTTCTCAGCTTA+AGG + chr5.3:47780318-47780337 MS.gene035972:CDS 35.0%
GGTTGAAGTTTACAACATAC+CGG + chr5.3:47780903-47780922 MS.gene035972:CDS 35.0%
TACAATGAGAAAGGCTTAGA+AGG - chr5.3:47780707-47780726 None:intergenic 35.0%
TCTATCAAGTGCAAGAACAA+TGG - chr5.3:47780588-47780607 None:intergenic 35.0%
TGACAATTTCTCAGCTTAAG+GGG + chr5.3:47780320-47780339 MS.gene035972:CDS 35.0%
TTATCATAGGTTAACTGGGA+TGG + chr5.3:47780838-47780857 MS.gene035972:intron 35.0%
! AAGTCTAGGAGTTTTTCTTG+AGG - chr5.3:47780962-47780981 None:intergenic 35.0%
! ATGAAGATTGGTTGGAAATC+TGG + chr5.3:47780247-47780266 MS.gene035972:CDS 35.0%
! GTTTTCATCAAAAACTCTGC+AGG + chr5.3:47780022-47780041 MS.gene035972:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG - chr5.3:47779519-47779538 None:intergenic 35.0%
AAAATATGGCAGATATGCGC+CGG + chr5.3:47779491-47779510 MS.gene035972:CDS 40.0%
AAAGTTTACTACTTCCCACG+AGG + chr5.3:47779545-47779564 MS.gene035972:CDS 40.0%
ACGATCAAGAATGGTTCTTG+TGG - chr5.3:47779606-47779625 None:intergenic 40.0%
ATCCAAACGTAGTCAATCCT+TGG + chr5.3:47780878-47780897 MS.gene035972:CDS 40.0%
CAAGTTCACTACAGATGAAG+CGG + chr5.3:47780075-47780094 MS.gene035972:CDS 40.0%
CAGTTTGGTAAAGACCTCAT+CGG - chr5.3:47779687-47779706 None:intergenic 40.0%
GATCTTTCCCAATCTTGCTT+TGG + chr5.3:47779859-47779878 MS.gene035972:CDS 40.0%
TATATGTGTACCCCCATTCT+CGG - chr5.3:47779922-47779941 None:intergenic 40.0%
TCAGTAGATTACATACTGGC+TGG + chr5.3:47779954-47779973 MS.gene035972:CDS 40.0%
TCTGCAGGAAAGATATCTGT+CGG + chr5.3:47780037-47780056 MS.gene035972:CDS 40.0%
TGACTACGTTTGGATTCTGT+GGG - chr5.3:47780873-47780892 None:intergenic 40.0%
TTGACTACGTTTGGATTCTG+TGG - chr5.3:47780874-47780893 None:intergenic 40.0%
TTGTAAACTTCAACCTGCCA+AGG - chr5.3:47780898-47780917 None:intergenic 40.0%
TTGTGTGAGAGTCTTGACAT+AGG - chr5.3:47779792-47779811 None:intergenic 40.0%
! ATCGAGTTAGAGCATTCGAT+AGG - chr5.3:47779842-47779861 None:intergenic 40.0%
! GCGCATATCTGCCATATTTT+GGG - chr5.3:47779491-47779510 None:intergenic 40.0%
! TCTAGGAGTTTTTCTTGAGG+CGG - chr5.3:47780959-47780978 None:intergenic 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG - chr5.3:47779518-47779537 None:intergenic 40.0%
!!! GATTAAGGTTTTGGATGCAG+TGG + chr5.3:47780126-47780145 MS.gene035972:CDS 40.0%
AAACGTAGTCAATCCTTGGC+AGG + chr5.3:47780882-47780901 MS.gene035972:CDS 45.0%
ACCTTAAAAGCCGAGAATGG+GGG + chr5.3:47779909-47779928 MS.gene035972:CDS 45.0%
ACGATGCCATGAAGATTGGT+TGG + chr5.3:47780239-47780258 MS.gene035972:CDS 45.0%
ACTACAGATGAAGCGGATGA+AGG + chr5.3:47780082-47780101 MS.gene035972:CDS 45.0%
AGACTCGTCCAAAGCAAGAT+TGG - chr5.3:47779870-47779889 None:intergenic 45.0%
AGATATCTGTCGGAATTCGC+AGG + chr5.3:47780047-47780066 MS.gene035972:CDS 45.0%
CGACCTTAAAAGCCGAGAAT+GGG + chr5.3:47779907-47779926 MS.gene035972:CDS 45.0%
CGATGCATAGAATGGATGGA+CGG - chr5.3:47779631-47779650 None:intergenic 45.0%
CGCTTCATCTGTAGTGAACT+TGG - chr5.3:47780077-47780096 None:intergenic 45.0%
CTCGTGTGGAACCCAAAATA+TGG + chr5.3:47779477-47779496 MS.gene035972:CDS 45.0%
GACCTTAAAAGCCGAGAATG+GGG + chr5.3:47779908-47779927 MS.gene035972:CDS 45.0%
GACTCGTCCAAAGCAAGATT+GGG - chr5.3:47779869-47779888 None:intergenic 45.0%
GAGACGATGCATAGAATGGA+TGG - chr5.3:47779635-47779654 None:intergenic 45.0%
GATGAACTCTCAGCAAGTCT+AGG - chr5.3:47780976-47780995 None:intergenic 45.0%
GGAAGAGACGATGCATAGAA+TGG - chr5.3:47779639-47779658 None:intergenic 45.0%
GTTGACGATGCCATGAAGAT+TGG + chr5.3:47780235-47780254 MS.gene035972:CDS 45.0%
TGCCAAGGATTGACTACGTT+TGG - chr5.3:47780883-47780902 None:intergenic 45.0%
TGGACGGTAACGATCAAGAA+TGG - chr5.3:47779615-47779634 None:intergenic 45.0%
TGTCTCCAACATCGTCTTCT+TGG - chr5.3:47779760-47779779 None:intergenic 45.0%
TTCATCACCCCAACAAACCT+TGG + chr5.3:47780990-47781009 MS.gene035972:CDS 45.0%
TTTGGATGCAGTGGAATTGG+CGG + chr5.3:47780135-47780154 MS.gene035972:CDS 45.0%
! GGCGCATATCTGCCATATTT+TGG - chr5.3:47779492-47779511 None:intergenic 45.0%
! TAGTGGTTGGCGTGATTTTG+TGG + chr5.3:47780198-47780217 MS.gene035972:CDS 45.0%
! TTTTCTTGAGGCGGAGTTGA+CGG - chr5.3:47780950-47780969 None:intergenic 45.0%
!! TCTTTGGAATCTTGACGTCG+GGG - chr5.3:47779517-47779536 None:intergenic 45.0%
!!! CATATTTTGGGTTCCACACG+AGG - chr5.3:47779479-47779498 None:intergenic 45.0%
!!! CCATTCTCGGCTTTTAAGGT+CGG - chr5.3:47779909-47779928 None:intergenic 45.0%
!!! GGTTTTGGATGCAGTGGAAT+TGG + chr5.3:47780132-47780151 MS.gene035972:CDS 45.0%
ACAGATGAAGCGGATGAAGG+AGG + chr5.3:47780085-47780104 MS.gene035972:CDS 50.0%
ACCTCAAGCATGCGCCAATT+AGG - chr5.3:47780382-47780401 None:intergenic 50.0%
ACTATCCGACAGCTAGTGGT+TGG + chr5.3:47780185-47780204 MS.gene035972:CDS 50.0%
ATGGTTCTTGTGGCAGCAGT+AGG - chr5.3:47779596-47779615 None:intergenic 50.0%
CATGTTCCAGATGACCTCGT+GGG - chr5.3:47779562-47779581 None:intergenic 50.0%
CCGACCTTAAAAGCCGAGAA+TGG + chr5.3:47779906-47779925 MS.gene035972:CDS 50.0%
CCTCAAGCATGCGCCAATTA+GGG - chr5.3:47780381-47780400 None:intergenic 50.0%
CTTGGTCAGTTACTGGAGTC+AGG - chr5.3:47779712-47779731 None:intergenic 50.0%
GTAAAGACCTCATCGGTGTG+AGG - chr5.3:47779680-47779699 None:intergenic 50.0%
GTGTACTATCCGACAGCTAG+TGG + chr5.3:47780181-47780200 MS.gene035972:CDS 50.0%
TACTGGAGTCAGGAGCAGTT+TGG - chr5.3:47779702-47779721 None:intergenic 50.0%
TCCAACATCGTCTTCTTGGC+CGG - chr5.3:47779756-47779775 None:intergenic 50.0%
TGGTTCTTGTGGCAGCAGTA+GGG - chr5.3:47779595-47779614 None:intergenic 50.0%
TGTTGATCCTCACACCGATG+AGG + chr5.3:47779670-47779689 MS.gene035972:CDS 50.0%
! ACCCCCATTCTCGGCTTTTA+AGG - chr5.3:47779913-47779932 None:intergenic 50.0%
! TGGTTGGCGTGATTTTGTGG+TGG + chr5.3:47780201-47780220 MS.gene035972:CDS 50.0%
!!! ATTTTGGGTTCCACACGAGG+TGG - chr5.3:47779476-47779495 None:intergenic 50.0%
CACCCCAACAAACCTTGGCA+GGG + chr5.3:47780995-47781014 MS.gene035972:CDS 55.0%
CCAACATCGTCTTCTTGGCC+GGG - chr5.3:47779755-47779774 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTG+AGG + chr5.3:47780378-47780397 MS.gene035972:CDS 55.0%
CTACTTCCCACGAGGTCATC+TGG + chr5.3:47779553-47779572 MS.gene035972:CDS 55.0%
GCATGTTCCAGATGACCTCG+TGG - chr5.3:47779563-47779582 None:intergenic 55.0%
GGGGGTTCTTGGTCAGTTAC+TGG - chr5.3:47779719-47779738 None:intergenic 55.0%
GGTTCTTGTGGCAGCAGTAG+GGG - chr5.3:47779594-47779613 None:intergenic 55.0%
TCACCCCAACAAACCTTGGC+AGG + chr5.3:47780994-47781013 MS.gene035972:CDS 55.0%
TCACGCCAACCACTAGCTGT+CGG - chr5.3:47780193-47780212 None:intergenic 55.0%
TCGACCCTGCCAAGGTTTGT+TGG - chr5.3:47781002-47781021 None:intergenic 55.0%
TCTTCTTGGCCGGGAACTAC+AGG - chr5.3:47779746-47779765 None:intergenic 55.0%
! CGACCCTGCCAAGGTTTGTT+GGG - chr5.3:47781001-47781020 None:intergenic 55.0%
!! GACCCTGCCAAGGTTTGTTG+GGG - chr5.3:47781000-47781019 None:intergenic 55.0%
ACGGTGGAGGAGGAAGTCGA+AGG - chr5.3:47780931-47780950 None:intergenic 60.0%
AGTACCACTCGACCCTGCCA+AGG - chr5.3:47781010-47781029 None:intergenic 60.0%
CAAACCTTGGCAGGGTCGAG+TGG + chr5.3:47781003-47781022 MS.gene035972:CDS 60.0%
CCCGGCCAAGAAGACGATGT+TGG + chr5.3:47779752-47779771 MS.gene035972:CDS 60.0%
CTACAGGAGGAGGGGGTTCT+TGG - chr5.3:47779730-47779749 None:intergenic 60.0%
TCATCCTCTGCCACCTCGTG+TGG + chr5.3:47779463-47779482 MS.gene035972:CDS 60.0%
TCTTGAGGCGGAGTTGACGG+TGG - chr5.3:47780947-47780966 None:intergenic 60.0%
TCTTGGCCGGGAACTACAGG+AGG - chr5.3:47779743-47779762 None:intergenic 60.0%
TGGAATCTTGACGTCGGGGC+CGG - chr5.3:47779513-47779532 None:intergenic 60.0%
! GAGGAGGAAGTCGAAGGTGC+CGG - chr5.3:47780925-47780944 None:intergenic 60.0%
CCCCCTCCTCCTGTAGTTCC+CGG + chr5.3:47779734-47779753 MS.gene035972:CDS 65.0%
CCGGGAACTACAGGAGGAGG+GGG - chr5.3:47779737-47779756 None:intergenic 65.0%
GCCGGGAACTACAGGAGGAG+GGG - chr5.3:47779738-47779757 None:intergenic 65.0%
GGCCGGGAACTACAGGAGGA+GGG - chr5.3:47779739-47779758 None:intergenic 65.0%
GGTTCCACACGAGGTGGCAG+AGG - chr5.3:47779470-47779489 None:intergenic 65.0%
TGAGGCGGAGTTGACGGTGG+AGG - chr5.3:47780944-47780963 None:intergenic 65.0%
TGGCCGGGAACTACAGGAGG+AGG - chr5.3:47779740-47779759 None:intergenic 65.0%
GGCGGAGTTGACGGTGGAGG+AGG - chr5.3:47780941-47780960 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 47779458 47781044 47779458 ID=MS.gene035972
chr5.3 mRNA 47779458 47781044 47779458 ID=MS.gene035972.t1;Parent=MS.gene035972
chr5.3 exon 47779458 47780399 47779458 ID=MS.gene035972.t1.exon1;Parent=MS.gene035972.t1
chr5.3 CDS 47779458 47780399 47779458 ID=cds.MS.gene035972.t1;Parent=MS.gene035972.t1
chr5.3 exon 47780847 47781044 47780847 ID=MS.gene035972.t1.exon2;Parent=MS.gene035972.t1
chr5.3 CDS 47780847 47781044 47780847 ID=cds.MS.gene035972.t1;Parent=MS.gene035972.t1
Gene Sequence

>MS.gene035972

ATGTCTCATCCTCTGCCACCTCGTGTGGAACCCAAAATATGGCAGATATGCGCCGGCCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACGAGGTCATCTGGAACATGCATGTTCTTCCCCTACTGCTGCCACAAGAACCATTCTTGATCGTTACCGTCCATCCATTCTATGCATCGTCTCTTCCGTTGATCTCTTTGTTGATCCTCACACCGATGAGGTCTTTACCAAACTGCTCCTGACTCCAGTAACTGACCAAGAACCCCCTCCTCCTGTAGTTCCCGGCCAAGAAGACGATGTTGGAGACAATCTTGTTTCCTATGTCAAGACTCTCACACAATCTGATTGTGCAAGAGTATTATGTGTACCTATCGAATGCTCTAACTCGATCTTTCCCAATCTTGCTTTGGACGAGTCTCAGAGCATCACTGTCACCGACCTTAAAAGCCGAGAATGGGGGTACACATATACCTATGCAAATCTCAGTAGATTACATACTGGCTGGTTAAATTTTGTACGAGAGAAGAAACTCGTCGCCAACGATTCTGTTGTTTTCATCAAAAACTCTGCAGGAAAGATATCTGTCGGAATTCGCAGGAACACCAAGTTCACTACAGATGAAGCGGATGAAGGAGGCGAGAATTTAACTGATGAGATTAAGGTTTTGGATGCAGTGGAATTGGCGGAGAAGAACACAGCATTTGATGTTGTGTACTATCCGACAGCTAGTGGTTGGCGTGATTTTGTGGTGGATGCTAAGACTGTTGACGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGAGTGAAACTTCCTTTGAAGAAATATGAATCTTCAAGTTCAAAGATGACAATTTCTCAGCTTAAGGGGACAATCTCTTTTGTCTTTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTGAGGTACATATGCTTTCTTAATTTGGTCTGATTATATTTCAATTGTTTATAGTTGAATCCATGATAAGATTATTTGATTGATATATATGAGTGTTGTTGAAGTTAATACATGAAACTATAATTAGATGATACTTGATGATACAGAAAAATGTAACAGAATCAGATTACACTTGAAATATTATTAATTCCCATTGTTCTTGCACTTGATAGATATCTCATGACACTTAATGTTTACTTTATGTAATCAGATTGTTTTTATACGAAACTGCCAGTGATATTCACTAAAAATAGTTACGAAGTTTAAGAACCTTCTAAGCCTTTCTCATTGTAAATACTCCTAATGCTAAGTAGAAACTTTTGATGTCTAAATACATATATACTATATATATTTTATCAAATATTTCAAATTATTGATATTGATTCTTACTGTTTTTTATCTTTTTATCATAGGTTAACTGGGATGGACTTGATTTCCCACAGAATCCAAACGTAGTCAATCCTTGGCAGGTTGAAGTTTACAACATACCGGCACCTTCGACTTCCTCCTCCACCGTCAACTCCGCCTCAAGAAAAACTCCTAGACTTGCTGAGAGTTCATCACCCCAACAAACCTTGGCAGGGTCGAGTGGTACTTTGCCTGACACATAG
Protein sequence

>MS.gene035972.t1

MSHPLPPRVEPKIWQICAGPDVKIPKIHSKVYYFPRGHLEHACSSPTAATRTILDRYRPSILCIVSSVDLFVDPHTDEVFTKLLLTPVTDQEPPPPVVPGQEDDVGDNLVSYVKTLTQSDCARVLCVPIECSNSIFPNLALDESQSITVTDLKSREWGYTYTYANLSRLHTGWLNFVREKKLVANDSVVFIKNSAGKISVGIRRNTKFTTDEADEGGENLTDEIKVLDAVELAEKNTAFDVVYYPTASGWRDFVVDAKTVDDAMKIGWKSGMRVKLPLKKYESSSSKMTISQLKGTISFVFNDSSNVPNWRMLEVNWDGLDFPQNPNVVNPWQVEVYNIPAPSTSSSTVNSASRKTPRLAESSSPQQTLAGSSGTLPDT