AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035979

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035979.t1 MTR_5g060630 87.714 350 25 2 1 332 1 350 0.0 619
MS.gene035979.t1 MTR_5g460740 87.714 350 25 2 1 332 1 350 0.0 619
MS.gene035979.t1 MTR_5g061890 55.202 346 110 7 1 332 1 315 9.32e-108 321
MS.gene035979.t1 MTR_5g060780 54.624 346 112 7 1 332 1 315 1.18e-107 320
MS.gene035979.t1 MTR_5g061220 48.930 374 115 8 1 332 8 347 6.87e-107 323
MS.gene035979.t1 MTR_5g460920 54.624 346 112 7 1 332 1 315 1.22e-106 318
MS.gene035979.t1 MTR_1g058210 50.142 351 123 6 1 332 1 318 1.12e-100 305
MS.gene035979.t1 MTR_5g040880 43.642 346 135 10 7 332 6 311 2.32e-74 239
MS.gene035979.t1 MTR_2g024430 45.614 342 147 14 1 332 9 321 2.66e-73 236
MS.gene035979.t1 MTR_5g040740 45.322 342 149 12 1 332 1 314 3.95e-71 227
MS.gene035979.t1 MTR_5g082140 42.308 338 159 12 5 332 5 316 1.94e-70 227
MS.gene035979.t1 MTR_2g006380 39.714 350 143 13 6 332 14 318 2.88e-65 213
MS.gene035979.t1 MTR_2g006270 41.504 359 140 15 1 332 1 316 3.01e-65 213
MS.gene035979.t1 MTR_5g062970 49.107 224 66 7 9 213 35 229 3.45e-53 176
MS.gene035979.t1 MTR_3g073420 33.504 391 160 14 4 332 8 360 3.15e-47 169
MS.gene035979.t1 MTR_5g060770 41.637 281 81 7 63 331 9 218 6.37e-46 160
MS.gene035979.t1 MTR_2g094570 29.668 391 169 14 7 332 6 355 2.96e-41 153
MS.gene035979.t1 MTR_2g094570 29.668 391 169 14 7 332 6 355 3.65e-41 153
MS.gene035979.t1 MTR_2g094570 29.668 391 169 14 7 332 62 411 4.38e-41 153
MS.gene035979.t1 MTR_1g064430 29.008 393 177 14 7 332 22 379 5.02e-38 144
MS.gene035979.t1 MTR_1g064430 29.082 392 178 14 7 332 35 392 5.49e-38 144
MS.gene035979.t1 MTR_4g058930 28.831 385 198 11 1 332 1 362 1.66e-35 137
MS.gene035979.t1 MTR_4g058930 29.351 385 196 12 1 332 1 362 2.27e-35 136
MS.gene035979.t1 MTR_1g094960 28.198 383 180 13 9 332 16 362 1.58e-33 131
MS.gene035979.t1 MTR_4g021580 29.191 346 189 11 10 332 17 329 4.58e-33 129
MS.gene035979.t1 MTR_4g021580 29.191 346 189 11 10 332 17 329 6.45e-33 129
MS.gene035979.t1 MTR_2g043250 27.273 352 200 9 5 332 16 335 5.28e-32 127
MS.gene035979.t1 MTR_2g005240 28.653 349 184 12 12 332 23 334 2.05e-30 122
MS.gene035979.t1 MTR_4g124900 26.593 361 191 9 5 332 16 335 4.11e-30 122
MS.gene035979.t1 MTR_7g062540 27.586 348 188 12 12 332 14 324 4.18e-30 121
MS.gene035979.t1 MTR_4g124900 26.593 361 191 9 5 332 16 335 4.70e-30 121
MS.gene035979.t1 MTR_2g093740 28.571 357 185 14 12 332 56 378 7.92e-30 120
MS.gene035979.t1 MTR_5g062960 60.920 87 34 0 246 332 2 88 8.61e-30 113
MS.gene035979.t1 MTR_2g093740 28.571 357 185 14 12 332 56 378 1.11e-29 120
MS.gene035979.t1 MTR_2g093740 28.571 357 185 14 12 332 56 378 1.17e-29 120
MS.gene035979.t1 MTR_2g014770 27.083 336 227 7 3 332 44 367 1.98e-29 119
MS.gene035979.t1 MTR_2g014770 27.577 359 196 11 3 332 44 367 2.23e-29 119
MS.gene035979.t1 MTR_8g027440 26.377 345 195 12 12 332 29 338 6.63e-29 118
MS.gene035979.t1 MTR_7g101280 27.204 397 182 16 7 332 26 386 1.21e-28 117
MS.gene035979.t1 MTR_0041s0110 27.204 397 182 16 7 332 26 386 1.21e-28 117
MS.gene035979.t1 MTR_2g093740 27.297 370 186 14 12 332 56 391 3.81e-28 115
MS.gene035979.t1 MTR_2g093740 27.297 370 186 14 12 332 56 391 3.95e-28 115
MS.gene035979.t1 MTR_2g093740 27.297 370 186 14 12 332 56 391 4.45e-28 115
MS.gene035979.t1 MTR_8g100050 25.852 352 191 10 12 332 59 371 7.18e-28 115
MS.gene035979.t1 MTR_8g100050 25.852 352 191 10 12 332 59 371 8.71e-28 115
MS.gene035979.t1 MTR_7g101275 29.080 337 174 12 22 331 2 300 2.34e-27 111
MS.gene035979.t1 MTR_1g024025 28.329 353 190 13 7 331 38 355 2.48e-27 114
MS.gene035979.t1 MTR_5g076270 27.119 354 200 10 5 332 17 338 3.10e-27 113
MS.gene035979.t1 MTR_4g088210 27.097 310 186 7 12 300 38 328 4.04e-27 112
MS.gene035979.t1 MTR_4g088210 27.097 310 186 7 12 300 38 328 4.33e-27 112
MS.gene035979.t1 MTR_5g460910 32.982 285 110 7 47 331 10 213 5.17e-27 109
MS.gene035979.t1 MTR_3g064050 26.571 350 199 10 9 332 23 340 6.60e-27 112
MS.gene035979.t1 MTR_8g079492 27.826 345 185 11 5 322 16 323 7.91e-27 112
MS.gene035979.t1 MTR_8g079492 27.826 345 185 11 5 322 16 323 8.61e-27 112
MS.gene035979.t1 MTR_4g060460 27.476 313 182 8 12 300 54 345 1.03e-25 108
MS.gene035979.t1 MTR_4g060460 27.476 313 182 8 12 300 54 345 1.10e-25 108
MS.gene035979.t1 MTR_2g018690 27.246 345 188 10 5 322 16 324 1.13e-25 108
MS.gene035979.t1 MTR_8g101360 26.361 349 201 9 9 332 28 345 5.47e-24 103
MS.gene035979.t1 MTR_8g446900 23.140 242 166 6 58 293 9 236 2.59e-13 70.9
MS.gene035979.t1 MTR_2g093740 28.340 247 131 11 120 332 14 248 5.96e-13 70.1
MS.gene035979.t1 MTR_2g093740 28.340 247 131 11 120 332 14 248 6.48e-13 70.1
MS.gene035979.t1 MTR_2g093740 29.032 186 106 8 161 332 88 261 1.80e-12 68.9
MS.gene035979.t1 MTR_2g093740 29.032 186 106 8 161 332 88 261 1.85e-12 68.9
MS.gene035979.t1 MTR_8g079492 27.536 207 116 7 141 322 44 241 9.09e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035979.t1 AT1G77850 34.211 380 162 12 7 333 14 358 1.26e-52 183
MS.gene035979.t1 AT1G77850 34.211 380 162 12 7 333 14 358 1.35e-52 183
MS.gene035979.t1 AT2G46530 29.683 347 183 10 11 332 20 330 1.34e-37 142
MS.gene035979.t1 AT2G46530 29.683 347 183 10 11 332 41 351 2.07e-37 142
MS.gene035979.t1 AT2G28350 28.061 392 191 12 3 333 1 362 1.13e-35 137
MS.gene035979.t1 AT2G28350 28.061 392 191 12 3 333 1 362 1.24e-35 137
MS.gene035979.t1 AT4G30080 29.810 369 193 13 9 332 16 363 2.05e-35 137
MS.gene035979.t1 AT4G23980 29.073 313 167 8 12 300 12 293 2.14e-34 134
MS.gene035979.t1 AT4G23980 29.073 313 167 8 12 300 12 293 2.52e-34 133
MS.gene035979.t1 AT2G33860 29.226 349 185 11 12 332 54 368 1.18e-33 131
MS.gene035979.t1 AT3G61830 27.299 348 190 11 11 332 24 334 4.15e-33 129
MS.gene035979.t1 AT1G35520 28.739 341 210 10 12 344 27 342 2.54e-30 122
MS.gene035979.t1 AT1G59750 28.530 347 187 12 12 332 22 333 2.69e-30 122
MS.gene035979.t1 AT1G59750 28.530 347 187 12 12 332 22 333 2.70e-30 122
MS.gene035979.t1 AT1G59750 28.530 347 187 12 12 332 22 333 2.81e-30 122
MS.gene035979.t1 AT1G59750 28.530 347 187 12 12 332 22 333 2.81e-30 122
MS.gene035979.t1 AT1G34390 27.114 343 189 10 12 332 27 330 8.49e-30 120
MS.gene035979.t1 AT5G20730 28.125 352 197 8 5 332 17 336 1.14e-29 120
MS.gene035979.t1 AT5G62000 25.926 351 192 10 12 332 61 373 2.26e-29 119
MS.gene035979.t1 AT5G62000 25.926 351 192 10 12 332 61 373 2.42e-29 119
MS.gene035979.t1 AT5G62000 25.926 351 192 10 12 332 61 373 2.42e-29 119
MS.gene035979.t1 AT5G62000 25.926 351 192 10 12 332 61 373 2.42e-29 119
MS.gene035979.t1 AT5G62000 25.926 351 192 10 12 332 61 373 2.42e-29 119
MS.gene035979.t1 AT5G37020 26.571 350 199 10 9 332 19 336 2.63e-29 119
MS.gene035979.t1 AT5G37020 26.571 350 199 10 9 332 19 336 2.86e-29 119
MS.gene035979.t1 AT1G34310 28.736 348 185 12 9 332 24 332 6.87e-29 117
MS.gene035979.t1 AT1G34410 26.667 345 190 10 12 332 27 332 7.29e-29 117
MS.gene035979.t1 AT5G20730 28.045 353 197 9 5 332 17 337 4.16e-28 116
MS.gene035979.t1 AT5G20730 27.762 353 198 10 5 332 17 337 4.18e-28 116
MS.gene035979.t1 AT1G19220 25.852 352 205 9 5 332 16 335 4.55e-28 115
MS.gene035979.t1 AT1G35240 26.957 345 189 10 12 332 27 332 4.96e-28 115
MS.gene035979.t1 AT1G30330 28.446 341 185 11 5 320 18 324 1.28e-27 114
MS.gene035979.t1 AT1G30330 28.446 341 185 11 5 320 18 324 1.28e-27 114
MS.gene035979.t1 AT1G30330 28.446 341 185 11 5 320 16 322 1.42e-27 114
MS.gene035979.t1 AT5G60450 27.987 318 175 10 11 300 40 331 1.12e-26 111
MS.gene035979.t1 AT5G60450 27.987 318 175 10 11 300 64 355 1.61e-26 111
MS.gene035979.t1 AT1G35540 26.000 350 186 11 12 332 27 332 4.50e-26 109
MS.gene035979.t1 AT1G19850 26.870 361 184 14 9 332 51 368 3.42e-24 104
MS.gene035979.t1 AT1G43950 30.435 230 106 7 12 221 27 222 3.51e-24 99.4
MS.gene035979.t1 AT1G19850 26.870 361 184 14 9 332 40 357 3.63e-24 104
MS.gene035979.t1 AT1G34170 29.070 344 172 14 12 328 25 323 7.48e-23 99.8
MS.gene035979.t1 AT3G61830 26.174 298 160 9 58 332 23 283 3.59e-20 92.0
MS.gene035979.t1 AT1G34170 30.466 279 132 10 12 263 25 268 2.76e-19 89.0
MS.gene035979.t1 AT1G34170 30.466 279 132 10 12 263 25 268 3.26e-19 89.0
MS.gene035979.t1 AT2G33860 29.189 185 118 6 155 332 33 211 5.14e-17 82.4
MS.gene035979.t1 AT2G46530 30.275 218 123 7 137 332 33 243 3.42e-16 80.1
MS.gene035979.t1 AT5G37020 27.841 176 121 4 161 332 68 241 1.39e-13 72.4

Find 94 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAGAATGGTTGGGAATT+TGG 0.146103 5.3:+47670040 MS.gene035979:CDS
TGTTTCCATCGTTCTCTTCT+TGG 0.162944 5.3:-47669476 None:intergenic
GGCGCATATTTGCCATATTT+TGG 0.204114 5.3:-47669190 None:intergenic
CCTCCTTCCTCTTCAGTTGC+AGG 0.210601 5.3:-47669878 None:intergenic
GCATGTTCCAAGTGGCCTTC+TGG 0.242565 5.3:-47669261 None:intergenic
CATCTTATCTGTTGATCTTC+TGG 0.246519 5.3:+47669350 MS.gene035979:CDS
AGGAGCTGTGTTTCAATGTT+AGG 0.289094 5.3:-47669294 None:intergenic
CCTCAGGAGGAGCTGGTTCT+TGG 0.290438 5.3:-47669515 None:intergenic
GAGGAGCTGGTTCTTGCATT+TGG 0.339888 5.3:-47669440 None:intergenic
TCCTAGCTGGCGTATGCTTC+AGG 0.352282 5.3:+47670171 MS.gene035979:CDS
CATGTTCCAAGTGGCCTTCT+GGG 0.356154 5.3:-47669260 None:intergenic
TGTATTATCCAACGGCTAAT+TGG 0.357410 5.3:+47669978 MS.gene035979:CDS
ACGACATTCAAGACGTAGTC+TGG 0.358095 5.3:+47669699 MS.gene035979:CDS
AATTCCGACAAATATCTTTC+CGG 0.381216 5.3:-47669837 None:intergenic
CCATCGTTCTCTTCTTGGTC+GGG 0.392490 5.3:-47669471 None:intergenic
TCCATCGTTCTCTTCTTGGT+CGG 0.402482 5.3:-47669472 None:intergenic
GAGTCCACACGGCGTGGCTC+AGG 0.410289 5.3:-47669168 None:intergenic
CTCCTTCCTCTTCAGTTGCA+GGG 0.412592 5.3:-47669877 None:intergenic
GCTATCATCGTCTTCTTCAT+CGG 0.416752 5.3:-47669541 None:intergenic
GAGGAGCTGGTTCTTGGACT+TGG 0.420907 5.3:-47669509 None:intergenic
GCTGTCAAGATTCCGAAGAT+TGG 0.424603 5.3:+47669219 MS.gene035979:CDS
GGAGCTGTGTTTCAATGTTA+GGG 0.425269 5.3:-47669293 None:intergenic
CTATCATCGTCTTCTTCATC+GGG 0.426116 5.3:-47669540 None:intergenic
CGCCAGCTAGGAACATTAGA+AGG 0.426148 5.3:-47670160 None:intergenic
TCTTCTTGGTCGGGAAAGTC+AGG 0.427116 5.3:-47669462 None:intergenic
CGGAATCTTGACAGCAGGTC+CGG 0.431910 5.3:-47669211 None:intergenic
GCCGTGTGGACTCCAAAATA+TGG 0.441140 5.3:+47669178 MS.gene035979:CDS
AGGGAAAAGTGAGAATTTAA+CGG 0.444973 5.3:+47669898 MS.gene035979:CDS
AGTTAAAGATGCTGTGGAAT+TGG 0.445090 5.3:+47669928 MS.gene035979:CDS
CGAATAGATTTGTGCACTAT+CGG 0.449107 5.3:+47670352 MS.gene035979:intron
ACACCACCATCGGTTGTTAC+CGG 0.450109 5.3:-47669417 None:intergenic
AAAAGTCCACTGTATCAGAT+TGG 0.451257 5.3:-47669596 None:intergenic
TCTTCTTCATCGGGAACCTC+AGG 0.467124 5.3:-47669531 None:intergenic
TACCGGAGTGAGGAGCAGTT+TGG 0.478735 5.3:-47669400 None:intergenic
ATGGAGGGCGAAGACATAGA+AGG 0.491229 5.3:-47669314 None:intergenic
CTACTTCCCAGAAGGCCACT+TGG 0.494993 5.3:+47669254 MS.gene035979:CDS
AAAGTCCACTGTATCAGATT+GGG 0.495652 5.3:-47669595 None:intergenic
GCTTCTTACTTTAGATCAGA+AGG 0.500774 5.3:+47670383 MS.gene035979:CDS
AATGGTTGGGAATTTGGAAT+GGG 0.505443 5.3:+47670046 MS.gene035979:CDS
GAAGACATATTATCAGCCTA+AGG 0.506655 5.3:+47670111 MS.gene035979:CDS
GAATGGTTGGGAATTTGGAA+TGG 0.509049 5.3:+47670045 MS.gene035979:CDS
TTCCTTCTAATGTTCCTAGC+TGG 0.513382 5.3:+47670158 MS.gene035979:CDS
TCGGGAACCTCAGGAGGAGC+TGG 0.515759 5.3:-47669522 None:intergenic
AAAGATGCTGTGGAATTGGC+TGG 0.518790 5.3:+47669932 MS.gene035979:CDS
ATGTTAGAGATTGTCCCCTT+AGG 0.526512 5.3:-47670127 None:intergenic
ATGAGGCTATGAAGAATGGT+TGG 0.527470 5.3:+47670032 MS.gene035979:CDS
TCGGGAAAGTCAGGAGGAGC+TGG 0.536223 5.3:-47669453 None:intergenic
AAGAAACTCAGGTTTACTAC+TGG 0.537296 5.3:+47669746 MS.gene035979:CDS
ATCTTCGGAATCTTGACAGC+AGG 0.537862 5.3:-47669216 None:intergenic
TTGCATTTGGACACCACCAT+CGG 0.539773 5.3:-47669427 None:intergenic
GAGAGCTTCTGAGACTGCAT+TGG 0.543170 5.3:-47669671 None:intergenic
GTTGATGAGGCTATGAAGAA+TGG 0.545415 5.3:+47670028 MS.gene035979:CDS
TCTGCCGGAAAGATATTTGT+CGG 0.547107 5.3:+47669833 MS.gene035979:CDS
TCAAGTTGTGTATTATCCAA+CGG 0.549124 5.3:+47669970 MS.gene035979:CDS
AACTCAGGTTTACTACTGGT+TGG 0.550552 5.3:+47669750 MS.gene035979:CDS
TGCCAAACTGCTCCTCACTC+CGG 0.557138 5.3:+47669398 MS.gene035979:CDS
AAGTGAGAATTTAACGGAGA+AGG 0.558251 5.3:+47669904 MS.gene035979:CDS
TCTGCCTGAGCCACGCCGTG+TGG 0.560213 5.3:+47669164 MS.gene035979:CDS
AAGACATATTATCAGCCTAA+GGG 0.565438 5.3:+47670112 MS.gene035979:CDS
TGAGGCTATGAAGAATGGTT+GGG 0.565444 5.3:+47670033 MS.gene035979:CDS
AGATATTTGTCGGAATTCGA+AGG 0.569711 5.3:+47669843 MS.gene035979:CDS
TTGGGTGAGAATCTTTACAT+AGG 0.574104 5.3:-47669577 None:intergenic
CTGCAACTGAAGAGGAAGGA+GGG 0.579420 5.3:+47669879 MS.gene035979:CDS
AAGATGATGCAAAGAACGGA+TGG 0.585879 5.3:-47669333 None:intergenic
TCTCACCCAATCTGATACAG+TGG 0.589102 5.3:+47669590 MS.gene035979:CDS
AAAATATGGCAAATATGCGC+CGG 0.590046 5.3:+47669192 MS.gene035979:CDS
AGATAAGATGATGCAAAGAA+CGG 0.590314 5.3:-47669337 None:intergenic
TCTTCATCGGGAACCTCAGG+AGG 0.597687 5.3:-47669528 None:intergenic
AAATCACACCAATTAGCCGT+TGG 0.602696 5.3:-47669986 None:intergenic
TATGTACCCTGCAACTGAAG+AGG 0.603746 5.3:+47669871 MS.gene035979:CDS
CTGGTTGGTCACAGTTTGTG+AGG 0.607267 5.3:+47669765 MS.gene035979:CDS
ATGATGCAAAGAACGGATGG+AGG 0.607808 5.3:-47669330 None:intergenic
CTCACTCCGGTAACAACCGA+TGG 0.610328 5.3:+47669411 MS.gene035979:CDS
TGATGCAAAGAACGGATGGA+GGG 0.611540 5.3:-47669329 None:intergenic
GAAGGCAGTTAAAGATGCTG+TGG 0.623830 5.3:+47669922 MS.gene035979:CDS
ATTTGTCGGAATTCGAAGGA+AGG 0.624742 5.3:+47669847 MS.gene035979:CDS
ACCTGAAGCATACGCCAGCT+AGG 0.625148 5.3:-47670172 None:intergenic
GAGCTGTGTTTCAATGTTAG+GGG 0.627335 5.3:-47669292 None:intergenic
AAAGTCTACTACTTCCCAGA+AGG 0.628017 5.3:+47669246 MS.gene035979:CDS
GCTACCACGTGAAGAAACTC+AGG 0.629119 5.3:+47669735 MS.gene035979:CDS
GCGTGGCTCAGGCAGAGACA+TGG 0.631977 5.3:-47669157 None:intergenic
GGAATTGGCTGGGAAGAACA+TGG 0.633030 5.3:+47669943 MS.gene035979:CDS
AGACATATTATCAGCCTAAG+GGG 0.634100 5.3:+47670113 MS.gene035979:CDS
TACCCTGCAACTGAAGAGGA+AGG 0.635196 5.3:+47669875 MS.gene035979:CDS
CATCGGTTGTTACCGGAGTG+AGG 0.640720 5.3:-47669410 None:intergenic
CCAAGAACCAGCTCCTCCTG+AGG 0.640922 5.3:+47669515 MS.gene035979:CDS
CCTGCAACTGAAGAGGAAGG+AGG 0.644773 5.3:+47669878 MS.gene035979:CDS
AAGATGCTGTGGAATTGGCT+GGG 0.646479 5.3:+47669933 MS.gene035979:CDS
GGATGCGAGTGTTGTTGATG+AGG 0.647140 5.3:+47670015 MS.gene035979:CDS
TCTTGGTCGGGAAAGTCAGG+AGG 0.656368 5.3:-47669459 None:intergenic
ACTCCGGTAACAACCGATGG+TGG 0.663896 5.3:+47669414 MS.gene035979:CDS
TAAACCTGAGTTTCTTCACG+TGG 0.666809 5.3:-47669739 None:intergenic
CCCGACCAAGAAGAGAACGA+TGG 0.669874 5.3:+47669471 MS.gene035979:CDS
ATGAACAAGCATGTTCCAAG+TGG 0.689657 5.3:-47669269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGCACTTGAAATATAAT+AGG - chr5.3:47670207-47670226 None:intergenic 20.0%
!! TTGAAACTAAAATTAGATGA+TGG + chr5.3:47670278-47670297 MS.gene035979:intron 20.0%
!! TTTCAACATCTAAATAAATC+AGG - chr5.3:47670264-47670283 None:intergenic 20.0%
!! GTTTTCATAAAAAAATCTGC+CGG + chr5.3:47669818-47669837 MS.gene035979:CDS 25.0%
AAGACATATTATCAGCCTAA+GGG + chr5.3:47670112-47670131 MS.gene035979:CDS 30.0%
AATTCCGACAAATATCTTTC+CGG - chr5.3:47669840-47669859 None:intergenic 30.0%
AGATAAGATGATGCAAAGAA+CGG - chr5.3:47669340-47669359 None:intergenic 30.0%
AGGGAAAAGTGAGAATTTAA+CGG + chr5.3:47669898-47669917 MS.gene035979:CDS 30.0%
! TCAAGTTGTGTATTATCCAA+CGG + chr5.3:47669970-47669989 MS.gene035979:CDS 30.0%
AAAAGTCCACTGTATCAGAT+TGG - chr5.3:47669599-47669618 None:intergenic 35.0%
AAAATATGGCAAATATGCGC+CGG + chr5.3:47669192-47669211 MS.gene035979:CDS 35.0%
AAAGTCCACTGTATCAGATT+GGG - chr5.3:47669598-47669617 None:intergenic 35.0%
AAGAAACTCAGGTTTACTAC+TGG + chr5.3:47669746-47669765 MS.gene035979:CDS 35.0%
AAGTGAGAATTTAACGGAGA+AGG + chr5.3:47669904-47669923 MS.gene035979:CDS 35.0%
AGACATATTATCAGCCTAAG+GGG + chr5.3:47670113-47670132 MS.gene035979:CDS 35.0%
AGATATTTGTCGGAATTCGA+AGG + chr5.3:47669843-47669862 MS.gene035979:CDS 35.0%
AGTTAAAGATGCTGTGGAAT+TGG + chr5.3:47669928-47669947 MS.gene035979:CDS 35.0%
ATCAATTCAATGCATTCACG+AGG - chr5.3:47669626-47669645 None:intergenic 35.0%
ATGAAGAATGGTTGGGAATT+TGG + chr5.3:47670040-47670059 MS.gene035979:CDS 35.0%
CAGTTTGGCAAAAACTTCAT+CGG - chr5.3:47669388-47669407 None:intergenic 35.0%
CATCTTATCTGTTGATCTTC+TGG + chr5.3:47669350-47669369 MS.gene035979:CDS 35.0%
CGAATAGATTTGTGCACTAT+CGG + chr5.3:47670352-47670371 MS.gene035979:intron 35.0%
GAAGACATATTATCAGCCTA+AGG + chr5.3:47670111-47670130 MS.gene035979:CDS 35.0%
GCTTCTTACTTTAGATCAGA+AGG + chr5.3:47670383-47670402 MS.gene035979:CDS 35.0%
TGTATTATCCAACGGCTAAT+TGG + chr5.3:47669978-47669997 MS.gene035979:CDS 35.0%
TTGGGTGAGAATCTTTACAT+AGG - chr5.3:47669580-47669599 None:intergenic 35.0%
! AATGGTTGGGAATTTGGAAT+GGG + chr5.3:47670046-47670065 MS.gene035979:CDS 35.0%
! TAATTGGTGTGATTTTGTGG+TGG + chr5.3:47669994-47670013 MS.gene035979:CDS 35.0%
! TAGACTTTTGAGCCAATCTT+CGG - chr5.3:47669234-47669253 None:intergenic 35.0%
AAAGTCTACTACTTCCCAGA+AGG + chr5.3:47669246-47669265 MS.gene035979:CDS 40.0%
AAATCACACCAATTAGCCGT+TGG - chr5.3:47669989-47670008 None:intergenic 40.0%
AACTCAGGTTTACTACTGGT+TGG + chr5.3:47669750-47669769 MS.gene035979:CDS 40.0%
AAGATGATGCAAAGAACGGA+TGG - chr5.3:47669336-47669355 None:intergenic 40.0%
AGGAGCTGTGTTTCAATGTT+AGG - chr5.3:47669297-47669316 None:intergenic 40.0%
ATGAACAAGCATGTTCCAAG+TGG - chr5.3:47669272-47669291 None:intergenic 40.0%
ATGAGGCTATGAAGAATGGT+TGG + chr5.3:47670032-47670051 MS.gene035979:CDS 40.0%
ATGTTAGAGATTGTCCCCTT+AGG - chr5.3:47670130-47670149 None:intergenic 40.0%
ATTTGTCGGAATTCGAAGGA+AGG + chr5.3:47669847-47669866 MS.gene035979:CDS 40.0%
CTATCATCGTCTTCTTCATC+GGG - chr5.3:47669543-47669562 None:intergenic 40.0%
GAATGGTTGGGAATTTGGAA+TGG + chr5.3:47670045-47670064 MS.gene035979:CDS 40.0%
GAGCTGTGTTTCAATGTTAG+GGG - chr5.3:47669295-47669314 None:intergenic 40.0%
GCTATCATCGTCTTCTTCAT+CGG - chr5.3:47669544-47669563 None:intergenic 40.0%
GGAGCTGTGTTTCAATGTTA+GGG - chr5.3:47669296-47669315 None:intergenic 40.0%
TAAACCTGAGTTTCTTCACG+TGG - chr5.3:47669742-47669761 None:intergenic 40.0%
TCTGCCGGAAAGATATTTGT+CGG + chr5.3:47669833-47669852 MS.gene035979:CDS 40.0%
TGAGGCTATGAAGAATGGTT+GGG + chr5.3:47670033-47670052 MS.gene035979:CDS 40.0%
TGTTTCCATCGTTCTCTTCT+TGG - chr5.3:47669479-47669498 None:intergenic 40.0%
TTCCTTCTAATGTTCCTAGC+TGG + chr5.3:47670158-47670177 MS.gene035979:CDS 40.0%
! GATCTTTCCCAATGTTGACT+TGG + chr5.3:47669643-47669662 MS.gene035979:intron 40.0%
! GCGTATGCTTCAGGTATTTT+TGG + chr5.3:47670180-47670199 MS.gene035979:intron 40.0%
! GGCGCATATTTGCCATATTT+TGG - chr5.3:47669193-47669212 None:intergenic 40.0%
! GGCTAATTGGTGTGATTTTG+TGG + chr5.3:47669991-47670010 MS.gene035979:CDS 40.0%
! GTTGATGAGGCTATGAAGAA+TGG + chr5.3:47670028-47670047 MS.gene035979:CDS 40.0%
AAAGATGCTGTGGAATTGGC+TGG + chr5.3:47669932-47669951 MS.gene035979:CDS 45.0%
AAGATGCTGTGGAATTGGCT+GGG + chr5.3:47669933-47669952 MS.gene035979:CDS 45.0%
ACGACATTCAAGACGTAGTC+TGG + chr5.3:47669699-47669718 MS.gene035979:CDS 45.0%
ATCTTCGGAATCTTGACAGC+AGG - chr5.3:47669219-47669238 None:intergenic 45.0%
ATGATGCAAAGAACGGATGG+AGG - chr5.3:47669333-47669352 None:intergenic 45.0%
GAAGGCAGTTAAAGATGCTG+TGG + chr5.3:47669922-47669941 MS.gene035979:CDS 45.0%
GCAAAAACTTCATCGGTGTG+AGG - chr5.3:47669381-47669400 None:intergenic 45.0%
GCTGTCAAGATTCCGAAGAT+TGG + chr5.3:47669219-47669238 MS.gene035979:CDS 45.0%
GGATCCTCCAAGTCAACATT+GGG - chr5.3:47669653-47669672 None:intergenic 45.0%
TATGTACCCTGCAACTGAAG+AGG + chr5.3:47669871-47669890 MS.gene035979:CDS 45.0%
TCCATCGTTCTCTTCTTGGT+CGG - chr5.3:47669475-47669494 None:intergenic 45.0%
TCTCACCCAATCTGATACAG+TGG + chr5.3:47669590-47669609 MS.gene035979:CDS 45.0%
TGATGCAAAGAACGGATGGA+GGG - chr5.3:47669332-47669351 None:intergenic 45.0%
TGGATCCTCCAAGTCAACAT+TGG - chr5.3:47669654-47669673 None:intergenic 45.0%
TTGCATTTGGACACCACCAT+CGG - chr5.3:47669430-47669449 None:intergenic 45.0%
!! CTTTCCCAATGTTGACTTGG+AGG + chr5.3:47669646-47669665 MS.gene035979:intron 45.0%
!!! GCCATATTTTGGAGTCCACA+CGG - chr5.3:47669182-47669201 None:intergenic 45.0%
ATGGAGGGCGAAGACATAGA+AGG - chr5.3:47669317-47669336 None:intergenic 50.0%
CATGTTCCAAGTGGCCTTCT+GGG - chr5.3:47669263-47669282 None:intergenic 50.0%
CCATCGTTCTCTTCTTGGTC+GGG - chr5.3:47669474-47669493 None:intergenic 50.0%
CGCCAGCTAGGAACATTAGA+AGG - chr5.3:47670163-47670182 None:intergenic 50.0%
CTCCTTCCTCTTCAGTTGCA+GGG - chr5.3:47669880-47669899 None:intergenic 50.0%
CTGCAACTGAAGAGGAAGGA+GGG + chr5.3:47669879-47669898 MS.gene035979:CDS 50.0%
GCCGTGTGGACTCCAAAATA+TGG + chr5.3:47669178-47669197 MS.gene035979:CDS 50.0%
GCTACCACGTGAAGAAACTC+AGG + chr5.3:47669735-47669754 MS.gene035979:CDS 50.0%
GGAATTGGCTGGGAAGAACA+TGG + chr5.3:47669943-47669962 MS.gene035979:CDS 50.0%
TACCCTGCAACTGAAGAGGA+AGG + chr5.3:47669875-47669894 MS.gene035979:CDS 50.0%
TCTTCTTCATCGGGAACCTC+AGG - chr5.3:47669534-47669553 None:intergenic 50.0%
TCTTCTTGGTCGGGAAAGTC+AGG - chr5.3:47669465-47669484 None:intergenic 50.0%
! ACACCACCATCGGTTGTTAC+CGG - chr5.3:47669420-47669439 None:intergenic 50.0%
! CTGGTTGGTCACAGTTTGTG+AGG + chr5.3:47669765-47669784 MS.gene035979:CDS 50.0%
! GAGGAGCTGGTTCTTGCATT+TGG - chr5.3:47669443-47669462 None:intergenic 50.0%
! GGATGCGAGTGTTGTTGATG+AGG + chr5.3:47670015-47670034 MS.gene035979:CDS 50.0%
!! GAGAGCTTCTGAGACTGCAT+TGG - chr5.3:47669674-47669693 None:intergenic 50.0%
ACCTGAAGCATACGCCAGCT+AGG - chr5.3:47670175-47670194 None:intergenic 55.0%
ACTCCGGTAACAACCGATGG+TGG + chr5.3:47669414-47669433 MS.gene035979:CDS 55.0%
CCCGACCAAGAAGAGAACGA+TGG + chr5.3:47669471-47669490 MS.gene035979:CDS 55.0%
CCTCCTTCCTCTTCAGTTGC+AGG - chr5.3:47669881-47669900 None:intergenic 55.0%
CCTGCAACTGAAGAGGAAGG+AGG + chr5.3:47669878-47669897 MS.gene035979:CDS 55.0%
CGGAATCTTGACAGCAGGTC+CGG - chr5.3:47669214-47669233 None:intergenic 55.0%
CTACTTCCCAGAAGGCCACT+TGG + chr5.3:47669254-47669273 MS.gene035979:CDS 55.0%
CTCACTCCGGTAACAACCGA+TGG + chr5.3:47669411-47669430 MS.gene035979:CDS 55.0%
GCATGTTCCAAGTGGCCTTC+TGG - chr5.3:47669264-47669283 None:intergenic 55.0%
TACCGGAGTGAGGAGCAGTT+TGG - chr5.3:47669403-47669422 None:intergenic 55.0%
TCCTAGCTGGCGTATGCTTC+AGG + chr5.3:47670171-47670190 MS.gene035979:CDS 55.0%
TCTTCATCGGGAACCTCAGG+AGG - chr5.3:47669531-47669550 None:intergenic 55.0%
TCTTGGTCGGGAAAGTCAGG+AGG - chr5.3:47669462-47669481 None:intergenic 55.0%
TGCCAAACTGCTCCTCACTC+CGG + chr5.3:47669398-47669417 MS.gene035979:CDS 55.0%
! CATCGGTTGTTACCGGAGTG+AGG - chr5.3:47669413-47669432 None:intergenic 55.0%
! GAGGAGCTGGTTCTTGGACT+TGG - chr5.3:47669512-47669531 None:intergenic 55.0%
!!! ATTTTGGAGTCCACACGGCG+TGG - chr5.3:47669177-47669196 None:intergenic 55.0%
CCAAGAACCAGCTCCTCCTG+AGG + chr5.3:47669515-47669534 MS.gene035979:CDS 60.0%
TCGGGAAAGTCAGGAGGAGC+TGG - chr5.3:47669456-47669475 None:intergenic 60.0%
! CCTCAGGAGGAGCTGGTTCT+TGG - chr5.3:47669518-47669537 None:intergenic 60.0%
TCGGGAACCTCAGGAGGAGC+TGG - chr5.3:47669525-47669544 None:intergenic 65.0%
GAGTCCACACGGCGTGGCTC+AGG - chr5.3:47669171-47669190 None:intergenic 70.0%
TCTGCCTGAGCCACGCCGTG+TGG + chr5.3:47669164-47669183 MS.gene035979:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 47669159 47670418 47669159 ID=MS.gene035979
chr5.3 mRNA 47669159 47670418 47669159 ID=MS.gene035979.t1;Parent=MS.gene035979
chr5.3 exon 47669159 47669626 47669159 ID=MS.gene035979.t1.exon1;Parent=MS.gene035979.t1
chr5.3 CDS 47669159 47669626 47669159 ID=cds.MS.gene035979.t1;Parent=MS.gene035979.t1
chr5.3 exon 47669668 47670192 47669668 ID=MS.gene035979.t1.exon2;Parent=MS.gene035979.t1
chr5.3 CDS 47669668 47670192 47669668 ID=cds.MS.gene035979.t1;Parent=MS.gene035979.t1
chr5.3 exon 47670359 47670418 47670359 ID=MS.gene035979.t1.exon3;Parent=MS.gene035979.t1
chr5.3 CDS 47670359 47670418 47670359 ID=cds.MS.gene035979.t1;Parent=MS.gene035979.t1
Gene Sequence

>MS.gene035979

ATGTCTCTGCCTGAGCCACGCCGTGTGGACTCCAAAATATGGCAAATATGCGCCGGACCTGCTGTCAAGATTCCGAAGATTGGCTCAAAAGTCTACTACTTCCCAGAAGGCCACTTGGAACATGCTTGTTCATCCCCTAACATTGAAACACAGCTCCTTCTATGTCTTCGCCCTCCATCCGTTCTTTGCATCATCTTATCTGTTGATCTTCTGGTAAATCCTCACACCGATGAAGTTTTTGCCAAACTGCTCCTCACTCCGGTAACAACCGATGGTGGTGTCCAAATGCAAGAACCAGCTCCTCCTGACTTTCCCGACCAAGAAGAGAACGATGGAAACAATCTTGTTGTCCAAGTCCAAGAACCAGCTCCTCCTGAGGTTCCCGATGAAGAAGACGATGATAGCAACAATGTTGTTTCCTATGTAAAGATTCTCACCCAATCTGATACAGTGGACTTTTTGTACCTCGTGAATGCATTGAATTGATCTTTCCCAATGTTGACTTGGAGGATCCAATGCAGTCTCAGAAGCTCTCAGACAACGACATTCAAGACGTAGTCTGGACGTACAAATATAGCTACCACGTGAAGAAACTCAGGTTTACTACTGGTTGGTCACAGTTTGTGAGGAAGAAGAAACTTGCTGCTCTTGATTCTGTTGTTTTCATAAAAAAATCTGCCGGAAAGATATTTGTCGGAATTCGAAGGAAGGCTATGTACCCTGCAACTGAAGAGGAAGGAGGGAAAAGTGAGAATTTAACGGAGAAGGCAGTTAAAGATGCTGTGGAATTGGCTGGGAAGAACATGGCATTTCAAGTTGTGTATTATCCAACGGCTAATTGGTGTGATTTTGTGGTGGATGCGAGTGTTGTTGATGAGGCTATGAAGAATGGTTGGGAATTTGGAATGGGAATCAAGCTTCGTTTGAATGAAGATGCATCTTCAAATTCAAAGAAGACATATTATCAGCCTAAGGGGACAATCTCTAACATGTCTAATGTTCCTTCTAATGTTCCTAGCTGGCGTATGCTTCAGGTATTTTTGGTCCTATTATATTTCAAGTGCATATTGATAAATATAATTTTGTAGTTGATACATTGATGCCTGATTTATTTAGATGTTGAAACTAAAATTAGATGATGGTTCATGAAATAGATGTCTAAAGTTTCGAGATTTAGTATGTTATTACTTTTTCGAATAGATTTGTGCACTATCGGCTATGTATGCTTCTTACTTTAGATCAGAAGGCTCTGATGCATAG
Protein sequence

>MS.gene035979.t1

MSLPEPRRVDSKIWQICAGPAVKIPKIGSKVYYFPEGHLEHACSSPNIETQLLLCLRPPSVLCIILSVDLLVNPHTDEVFAKLLLTPVTTDGGVQMQEPAPPDFPDQEENDGNNLVVQVQEPAPPEVPDEEDDDSNNVVSYVKILTQSDTVDFLYLDPMQSQKLSDNDIQDVVWTYKYSYHVKKLRFTTGWSQFVRKKKLAALDSVVFIKKSAGKIFVGIRRKAMYPATEEEGGKSENLTEKAVKDAVELAGKNMAFQVVYYPTANWCDFVVDASVVDEAMKNGWEFGMGIKLRLNEDASSNSKKTYYQPKGTISNMSNVPSNVPSWRMLQICALSAMYASYFRSEGSDA