Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03628.t1 | KEH33404.1 | 83.2 | 184 | 25 | 3 | 27 | 204 | 3 | 186 | 8.30E-75 | 290 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03628.t1 | A0A072UUA3 | 83.2 | 184 | 25 | 3 | 27 | 204 | 3 | 186 | 6.0e-75 | 290.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene03628.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03628.t1 | MTR_3g437790 | 83.152 | 184 | 25 | 3 | 27 | 204 | 3 | 186 | 4.77e-99 | 285 |
| MS.gene03628.t1 | MTR_2g045020 | 61.081 | 185 | 64 | 6 | 18 | 198 | 1 | 181 | 6.95e-59 | 183 |
| MS.gene03628.t1 | MTR_8g022970 | 50.857 | 175 | 73 | 6 | 32 | 197 | 10 | 180 | 2.52e-46 | 151 |
| MS.gene03628.t1 | MTR_3g109930 | 51.656 | 151 | 69 | 3 | 48 | 196 | 16 | 164 | 2.30e-44 | 146 |
| MS.gene03628.t1 | MTR_2g105290 | 36.025 | 161 | 95 | 3 | 31 | 185 | 3 | 161 | 2.42e-23 | 92.0 |
| MS.gene03628.t1 | MTR_7g028448 | 37.594 | 133 | 73 | 2 | 53 | 185 | 31 | 153 | 2.00e-21 | 87.0 |
| MS.gene03628.t1 | MTR_4g036915 | 37.594 | 133 | 73 | 2 | 53 | 185 | 31 | 153 | 2.00e-21 | 87.0 |
| MS.gene03628.t1 | MTR_8g086290 | 35.032 | 157 | 94 | 3 | 35 | 185 | 7 | 161 | 2.20e-20 | 84.3 |
| MS.gene03628.t1 | MTR_5g053390 | 36.774 | 155 | 86 | 4 | 38 | 182 | 7 | 159 | 2.59e-18 | 80.5 |
| MS.gene03628.t1 | MTR_4g127140 | 33.803 | 142 | 84 | 3 | 53 | 185 | 31 | 171 | 6.49e-17 | 75.5 |
| MS.gene03628.t1 | MTR_3g052920 | 32.911 | 158 | 88 | 3 | 38 | 182 | 7 | 159 | 3.43e-16 | 74.7 |
| MS.gene03628.t1 | MTR_8g043650 | 30.909 | 165 | 95 | 4 | 31 | 182 | 1 | 159 | 5.52e-16 | 73.9 |
| MS.gene03628.t1 | MTR_1g012570 | 32.298 | 161 | 85 | 5 | 38 | 182 | 7 | 159 | 1.86e-15 | 72.8 |
| MS.gene03628.t1 | MTR_1g105920 | 31.646 | 158 | 90 | 4 | 38 | 182 | 7 | 159 | 2.33e-15 | 72.4 |
| MS.gene03628.t1 | MTR_1g106070 | 31.013 | 158 | 91 | 3 | 38 | 182 | 7 | 159 | 2.39e-15 | 71.2 |
| MS.gene03628.t1 | MTR_3g030770 | 33.125 | 160 | 83 | 5 | 39 | 182 | 8 | 159 | 3.14e-15 | 72.0 |
| MS.gene03628.t1 | MTR_3g080940 | 29.861 | 144 | 83 | 3 | 53 | 186 | 28 | 163 | 7.72e-15 | 70.5 |
| MS.gene03628.t1 | MTR_1g105910 | 31.013 | 158 | 91 | 3 | 38 | 182 | 7 | 159 | 8.86e-15 | 70.9 |
| MS.gene03628.t1 | MTR_2g085250 | 29.375 | 160 | 91 | 4 | 38 | 182 | 7 | 159 | 1.32e-14 | 70.5 |
| MS.gene03628.t1 | MTR_2g085280 | 29.375 | 160 | 91 | 4 | 38 | 182 | 7 | 159 | 1.33e-14 | 70.1 |
| MS.gene03628.t1 | MTR_1g108510 | 32.500 | 160 | 84 | 5 | 39 | 182 | 8 | 159 | 2.82e-14 | 69.3 |
| MS.gene03628.t1 | MTR_2g030740 | 32.298 | 161 | 85 | 5 | 38 | 182 | 7 | 159 | 3.09e-14 | 69.3 |
| MS.gene03628.t1 | MTR_1g108580 | 32.500 | 160 | 84 | 5 | 39 | 182 | 8 | 159 | 1.02e-13 | 67.8 |
| MS.gene03628.t1 | MTR_8g046350 | 42.466 | 73 | 36 | 1 | 41 | 107 | 3 | 75 | 2.01e-13 | 65.5 |
| MS.gene03628.t1 | MTR_5g055100 | 32.298 | 161 | 83 | 6 | 38 | 181 | 7 | 158 | 3.16e-13 | 66.6 |
| MS.gene03628.t1 | MTR_0121s0080 | 33.824 | 136 | 78 | 4 | 54 | 182 | 29 | 159 | 9.75e-12 | 62.4 |
| MS.gene03628.t1 | MTR_3g052870 | 43.056 | 72 | 35 | 1 | 38 | 103 | 7 | 78 | 1.03e-11 | 62.0 |
| MS.gene03628.t1 | MTR_1g047550 | 34.615 | 104 | 60 | 2 | 41 | 136 | 3 | 106 | 1.22e-11 | 61.2 |
| MS.gene03628.t1 | MTR_8g051580 | 46.552 | 58 | 31 | 0 | 53 | 110 | 21 | 78 | 1.40e-11 | 61.2 |
| MS.gene03628.t1 | MTR_1g105905 | 30.216 | 139 | 79 | 4 | 53 | 181 | 11 | 141 | 1.54e-11 | 61.6 |
| MS.gene03628.t1 | MTR_1g077360 | 29.193 | 161 | 90 | 3 | 38 | 182 | 7 | 159 | 2.49e-11 | 61.2 |
| MS.gene03628.t1 | MTR_8g079502 | 30.909 | 110 | 68 | 2 | 41 | 142 | 3 | 112 | 2.61e-11 | 60.5 |
| MS.gene03628.t1 | MTR_2g093190 | 30.573 | 157 | 91 | 4 | 39 | 182 | 8 | 159 | 4.23e-11 | 60.5 |
| MS.gene03628.t1 | MTR_7g062350 | 33.577 | 137 | 79 | 3 | 53 | 182 | 40 | 171 | 8.14e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03628.t1 | AT5G60440 | 31.757 | 148 | 92 | 4 | 53 | 194 | 26 | 170 | 2.45e-16 | 75.9 |
| MS.gene03628.t1 | AT2G24840 | 28.090 | 178 | 106 | 5 | 25 | 192 | 63 | 228 | 4.49e-16 | 74.7 |
| MS.gene03628.t1 | AT3G66656 | 51.020 | 49 | 24 | 0 | 53 | 101 | 21 | 69 | 3.91e-12 | 62.4 |
| MS.gene03628.t1 | AT4G36590 | 28.571 | 161 | 97 | 5 | 38 | 182 | 5 | 163 | 9.39e-12 | 62.8 |
| MS.gene03628.t1 | AT2G34440 | 29.508 | 122 | 80 | 2 | 53 | 171 | 21 | 139 | 1.67e-11 | 60.8 |
| MS.gene03628.t1 | AT1G65360 | 29.114 | 158 | 93 | 5 | 38 | 182 | 5 | 156 | 2.77e-11 | 61.2 |
Find 33 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACACACATTCTCTTGAAT+TGG | 0.284587 | 3.4:+33921280 | None:intergenic |
| GAATAAATCTATTGATGATA+TGG | 0.314688 | 3.4:-33921395 | MS.gene03628:CDS |
| TGAATGACAGCATCATAGTT+AGG | 0.369392 | 3.4:+33921598 | None:intergenic |
| TCGCTTCATATCGAAGCCTT+AGG | 0.376463 | 3.4:+33921506 | None:intergenic |
| TGATATTGAATGAAACCAAC+TGG | 0.389424 | 3.4:-33921849 | MS.gene03628:CDS |
| TTGTTTCTCTCGGCCGCCAT+TGG | 0.404729 | 3.4:+33921784 | None:intergenic |
| CTTCCGTGTGTTGTTTCTCT+CGG | 0.413838 | 3.4:+33921774 | None:intergenic |
| CGATGAGTGCAGTTTCTGAT+TGG | 0.423120 | 3.4:+33921659 | None:intergenic |
| CGCTTCATATCGAAGCCTTA+GGG | 0.430275 | 3.4:+33921507 | None:intergenic |
| CTCATCCTCGTCCATATGTC+CGG | 0.445428 | 3.4:+33921564 | None:intergenic |
| CTTATTCATGTTGTCCCAGT+TGG | 0.447593 | 3.4:+33921834 | None:intergenic |
| CTTCTTCGCTTCGCTTGCTA+CGG | 0.451335 | 3.4:+33921315 | None:intergenic |
| GAAGGATGAAATCAAGAAAG+AGG | 0.477445 | 3.4:-33921746 | MS.gene03628:CDS |
| GATTTATTCCACCAATCAGC+AGG | 0.485193 | 3.4:+33921409 | None:intergenic |
| CTTCGATATGAAGCGATTCA+AGG | 0.502715 | 3.4:-33921499 | MS.gene03628:CDS |
| GAATGACAGCATCATAGTTA+GGG | 0.520135 | 3.4:+33921599 | None:intergenic |
| CTCTTCTTCAAGATCATGGA+TGG | 0.520427 | 3.4:-33922104 | None:intergenic |
| CATGCATAGAACTTGTTGTT+GGG | 0.522753 | 3.4:+33921628 | None:intergenic |
| AGGAAAACTTAAAGAAGAGA+AGG | 0.524858 | 3.4:-33921479 | MS.gene03628:CDS |
| CAACACACGGAAGAGAAAGA+AGG | 0.527351 | 3.4:-33921764 | MS.gene03628:CDS |
| CCGAGTTTGAGTTTCGAGAA+GGG | 0.532852 | 3.4:+33921718 | None:intergenic |
| TTGATTTGCCTGCTGATTGG+TGG | 0.538450 | 3.4:-33921417 | MS.gene03628:CDS |
| CCCTTCTCGAAACTCAAACT+CGG | 0.552196 | 3.4:-33921718 | MS.gene03628:CDS |
| TGGGACAACATGAATAAGAA+TGG | 0.556319 | 3.4:-33921829 | MS.gene03628:CDS |
| TCCGAGTTTGAGTTTCGAGA+AGG | 0.567859 | 3.4:+33921717 | None:intergenic |
| ATGATGTTGTTGAAACCCTA+AGG | 0.589934 | 3.4:-33921522 | MS.gene03628:CDS |
| ACATGCATAGAACTTGTTGT+TGG | 0.594378 | 3.4:+33921627 | None:intergenic |
| AACAACAAGTTCTATGCATG+TGG | 0.619639 | 3.4:-33921625 | MS.gene03628:CDS |
| GTCTAAGAAGAAAATTCCAA+TGG | 0.626985 | 3.4:-33921800 | MS.gene03628:CDS |
| TAAGAAGAAAATTCCAATGG+CGG | 0.679668 | 3.4:-33921797 | MS.gene03628:CDS |
| CGGCCGAGAGAAACAACACA+CGG | 0.685523 | 3.4:-33921777 | MS.gene03628:CDS |
| TCTGACCGGACATATGGACG+AGG | 0.696130 | 3.4:-33921569 | MS.gene03628:CDS |
| GATATTGAATGAAACCAACT+GGG | 0.707052 | 3.4:-33921848 | MS.gene03628:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATAAATCTATTGATGATA+TGG | - | chr3.4:33921973-33921992 | MS.gene03628:intron | 20.0% |
| !!! | TTGTTTTATGTGAATAAACT+TGG | + | chr3.4:33921418-33921437 | None:intergenic | 20.0% |
| AGGAAAACTTAAAGAAGAGA+AGG | - | chr3.4:33921889-33921908 | MS.gene03628:intron | 30.0% | |
| GATATTGAATGAAACCAACT+GGG | - | chr3.4:33921520-33921539 | MS.gene03628:CDS | 30.0% | |
| GTCTAAGAAGAAAATTCCAA+TGG | - | chr3.4:33921568-33921587 | MS.gene03628:CDS | 30.0% | |
| TAACACACATTCTCTTGAAT+TGG | + | chr3.4:33922091-33922110 | None:intergenic | 30.0% | |
| TAAGAAGAAAATTCCAATGG+CGG | - | chr3.4:33921571-33921590 | MS.gene03628:CDS | 30.0% | |
| TGATATTGAATGAAACCAAC+TGG | - | chr3.4:33921519-33921538 | MS.gene03628:CDS | 30.0% | |
| TTATGTGAATAAACTTGGCT+AGG | + | chr3.4:33921413-33921432 | None:intergenic | 30.0% | |
| AACAACAAGTTCTATGCATG+TGG | - | chr3.4:33921743-33921762 | MS.gene03628:CDS | 35.0% | |
| ATGATGTTGTTGAAACCCTA+AGG | - | chr3.4:33921846-33921865 | MS.gene03628:CDS | 35.0% | |
| GAAGGATGAAATCAAGAAAG+AGG | - | chr3.4:33921622-33921641 | MS.gene03628:CDS | 35.0% | |
| GAATGACAGCATCATAGTTA+GGG | + | chr3.4:33921772-33921791 | None:intergenic | 35.0% | |
| TGAATGACAGCATCATAGTT+AGG | + | chr3.4:33921773-33921792 | None:intergenic | 35.0% | |
| TGGGACAACATGAATAAGAA+TGG | - | chr3.4:33921539-33921558 | MS.gene03628:CDS | 35.0% | |
| TGTGTGATTTCAGACTTTGT+GGG | - | chr3.4:33921443-33921462 | MS.gene03628:CDS | 35.0% | |
| ! | TGATTAGGGTTTTTACCCTA+TGG | - | chr3.4:33921387-33921406 | MS.gene03628:CDS | 35.0% |
| !! | ACATGCATAGAACTTGTTGT+TGG | + | chr3.4:33921744-33921763 | None:intergenic | 35.0% |
| !! | CATGCATAGAACTTGTTGTT+GGG | + | chr3.4:33921743-33921762 | None:intergenic | 35.0% |
| !!! | ATTTTGATTTGCCTGCTGAT+TGG | - | chr3.4:33921948-33921967 | MS.gene03628:intron | 35.0% |
| AATAAACTTGGCTAGGCCAT+AGG | + | chr3.4:33921406-33921425 | None:intergenic | 40.0% | |
| ATAAACTTGGCTAGGCCATA+GGG | + | chr3.4:33921405-33921424 | None:intergenic | 40.0% | |
| CTTATTCATGTTGTCCCAGT+TGG | + | chr3.4:33921537-33921556 | None:intergenic | 40.0% | |
| CTTCGATATGAAGCGATTCA+AGG | - | chr3.4:33921869-33921888 | MS.gene03628:intron | 40.0% | |
| GATTTATTCCACCAATCAGC+AGG | + | chr3.4:33921962-33921981 | None:intergenic | 40.0% | |
| GTGTGTGATTTCAGACTTTG+TGG | - | chr3.4:33921442-33921461 | MS.gene03628:CDS | 40.0% | |
| ! | AACCAAGAAAGTGCGTGATT+AGG | - | chr3.4:33921372-33921391 | MS.gene03628:CDS | 40.0% |
| ! | ACCAAGAAAGTGCGTGATTA+GGG | - | chr3.4:33921373-33921392 | MS.gene03628:CDS | 40.0% |
| ACCCTAATCACGCACTTTCT+TGG | + | chr3.4:33921377-33921396 | None:intergenic | 45.0% | |
| CAACACACGGAAGAGAAAGA+AGG | - | chr3.4:33921604-33921623 | MS.gene03628:CDS | 45.0% | |
| CCCTTCTCGAAACTCAAACT+CGG | - | chr3.4:33921650-33921669 | MS.gene03628:CDS | 45.0% | |
| CGATGAGTGCAGTTTCTGAT+TGG | + | chr3.4:33921712-33921731 | None:intergenic | 45.0% | |
| CGCTTCATATCGAAGCCTTA+GGG | + | chr3.4:33921864-33921883 | None:intergenic | 45.0% | |
| TCGCTTCATATCGAAGCCTT+AGG | + | chr3.4:33921865-33921884 | None:intergenic | 45.0% | |
| ! | CCGAGTTTGAGTTTCGAGAA+GGG | + | chr3.4:33921653-33921672 | None:intergenic | 45.0% |
| ! | GTCATTCAGCGTTTTCTGAC+CGG | - | chr3.4:33921785-33921804 | MS.gene03628:CDS | 45.0% |
| ! | TCCGAGTTTGAGTTTCGAGA+AGG | + | chr3.4:33921654-33921673 | None:intergenic | 45.0% |
| !! | CTTCCGTGTGTTGTTTCTCT+CGG | + | chr3.4:33921597-33921616 | None:intergenic | 45.0% |
| !! | TTGATTTGCCTGCTGATTGG+TGG | - | chr3.4:33921951-33921970 | MS.gene03628:intron | 45.0% |
| CTCATCCTCGTCCATATGTC+CGG | + | chr3.4:33921807-33921826 | None:intergenic | 50.0% | |
| ! | CTTCTTCGCTTCGCTTGCTA+CGG | + | chr3.4:33922056-33922075 | None:intergenic | 50.0% |
| ! | GCGTTTTCTGACCGGACATA+TGG | - | chr3.4:33921793-33921812 | MS.gene03628:CDS | 50.0% |
| CGGCCGAGAGAAACAACACA+CGG | - | chr3.4:33921591-33921610 | MS.gene03628:CDS | 55.0% | |
| TCTGACCGGACATATGGACG+AGG | - | chr3.4:33921799-33921818 | MS.gene03628:CDS | 55.0% | |
| ! | TTGTTTCTCTCGGCCGCCAT+TGG | + | chr3.4:33921587-33921606 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 33921279 | 33922111 | 33921279 | ID=MS.gene03628 |
| chr3.4 | mRNA | 33921279 | 33922111 | 33921279 | ID=MS.gene03628.t1;Parent=MS.gene03628 |
| chr3.4 | exon | 33922105 | 33922111 | 33922105 | ID=MS.gene03628.t1.exon1;Parent=MS.gene03628.t1 |
| chr3.4 | CDS | 33922105 | 33922111 | 33922105 | ID=cds.MS.gene03628.t1;Parent=MS.gene03628.t1 |
| chr3.4 | exon | 33921279 | 33921886 | 33921279 | ID=MS.gene03628.t1.exon2;Parent=MS.gene03628.t1 |
| chr3.4 | CDS | 33921279 | 33921886 | 33921279 | ID=cds.MS.gene03628.t1;Parent=MS.gene03628.t1 |
| Gene Sequence |
| Protein sequence |
