Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036380.t1 | XP_024642224.1 | 81.5 | 205 | 36 | 1 | 1 | 205 | 1 | 203 | 4.10E-93 | 350.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036380.t1 | Q9SZL8 | 26.9 | 197 | 132 | 5 | 15 | 199 | 47 | 243 | 8.5e-15 | 82.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036380.t1 | A0A2K3JN18 | 71.2 | 205 | 55 | 1 | 1 | 205 | 1 | 201 | 7.0e-79 | 303.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene036380.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036380.t1 | MTR_0856s0010 | 78.395 | 162 | 33 | 1 | 1 | 162 | 1 | 160 | 1.29e-92 | 268 |
| MS.gene036380.t1 | MTR_7g115320 | 56.164 | 73 | 29 | 1 | 123 | 195 | 6 | 75 | 4.55e-23 | 89.0 |
| MS.gene036380.t1 | MTR_7g446060 | 40.449 | 89 | 51 | 1 | 111 | 199 | 1 | 87 | 1.75e-18 | 78.6 |
| MS.gene036380.t1 | MTR_7g074790 | 24.719 | 178 | 111 | 3 | 25 | 199 | 1 | 158 | 7.56e-15 | 70.9 |
| MS.gene036380.t1 | MTR_1g008290 | 33.636 | 110 | 70 | 1 | 96 | 205 | 14 | 120 | 1.39e-13 | 65.9 |
| MS.gene036380.t1 | MTR_2g014100 | 24.500 | 200 | 121 | 6 | 29 | 199 | 32 | 230 | 7.93e-12 | 62.8 |
| MS.gene036380.t1 | MTR_1g070355 | 29.050 | 179 | 112 | 7 | 42 | 208 | 80 | 255 | 1.90e-11 | 62.8 |
| MS.gene036380.t1 | MTR_1g083640 | 24.074 | 216 | 127 | 7 | 19 | 199 | 24 | 237 | 2.05e-11 | 62.0 |
| MS.gene036380.t1 | MTR_2g098920 | 35.185 | 108 | 68 | 2 | 35 | 140 | 79 | 186 | 2.12e-11 | 62.0 |
| MS.gene036380.t1 | MTR_0558s0020 | 22.768 | 224 | 139 | 7 | 8 | 199 | 1 | 222 | 2.57e-11 | 62.0 |
| MS.gene036380.t1 | MTR_1g019850 | 28.409 | 176 | 108 | 7 | 7 | 178 | 46 | 207 | 2.82e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036380.t1 | AT4G15090 | 29.524 | 210 | 136 | 7 | 5 | 205 | 18 | 224 | 4.79e-14 | 70.5 |
| MS.gene036380.t1 | AT4G15090 | 29.524 | 210 | 136 | 7 | 5 | 205 | 18 | 224 | 4.79e-14 | 70.5 |
| MS.gene036380.t1 | AT4G15090 | 29.524 | 210 | 136 | 7 | 5 | 205 | 18 | 224 | 4.79e-14 | 70.5 |
| MS.gene036380.t1 | AT4G15090 | 29.524 | 210 | 136 | 7 | 5 | 205 | 18 | 224 | 4.79e-14 | 70.5 |
| MS.gene036380.t1 | AT4G15090 | 29.524 | 210 | 136 | 7 | 5 | 205 | 18 | 224 | 4.79e-14 | 70.5 |
| MS.gene036380.t1 | AT2G43280 | 35.043 | 117 | 70 | 3 | 30 | 140 | 16 | 132 | 3.31e-13 | 66.2 |
| MS.gene036380.t1 | AT4G38180 | 28.261 | 184 | 124 | 4 | 24 | 199 | 60 | 243 | 3.79e-13 | 68.2 |
| MS.gene036380.t1 | AT3G07500 | 35.238 | 105 | 65 | 2 | 35 | 136 | 32 | 136 | 3.99e-11 | 60.5 |
| MS.gene036380.t1 | AT1G76320 | 27.976 | 168 | 112 | 4 | 40 | 205 | 1 | 161 | 6.91e-11 | 61.2 |
| MS.gene036380.t1 | AT1G76320 | 27.976 | 168 | 112 | 4 | 40 | 205 | 1 | 161 | 6.91e-11 | 61.2 |
| MS.gene036380.t1 | AT1G76320 | 27.976 | 168 | 112 | 4 | 40 | 205 | 1 | 161 | 7.58e-11 | 61.2 |
| MS.gene036380.t1 | AT1G76320 | 27.976 | 168 | 112 | 4 | 40 | 205 | 1 | 161 | 7.66e-11 | 61.2 |
Find 42 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCACATCTCTTTCATTAAA+TGG | 0.136344 | 2.4:-39358189 | None:intergenic |
| TTGAACAACCTTGCCTTTCT+AGG | 0.211285 | 2.4:-39358045 | None:intergenic |
| GTTCAAGGCCAACAAGAAAA+TGG | 0.356488 | 2.4:+39358062 | MS.gene036380:CDS |
| TGTATCAAATTGAAAGAAGA+TGG | 0.385199 | 2.4:+39357973 | MS.gene036380:CDS |
| AACAAGGAACGACGTGCAAT+TGG | 0.416589 | 2.4:+39358222 | MS.gene036380:CDS |
| ATTTGTCACTCTTTCAGTAT+TGG | 0.425839 | 2.4:-39358417 | None:intergenic |
| CAATCTCTGGTTAAAGATGC+AGG | 0.432888 | 2.4:+39358150 | MS.gene036380:CDS |
| TGAACAACCTTGCCTTTCTA+GGG | 0.439320 | 2.4:-39358044 | None:intergenic |
| AGAAAATGGACTTGCATGTA+AGG | 0.444990 | 2.4:+39358076 | MS.gene036380:CDS |
| CAACCTTGCCTTTCTAGGGC+TGG | 0.475224 | 2.4:-39358040 | None:intergenic |
| TTGAAGACACGTCCGACAAT+GGG | 0.482433 | 2.4:+39357928 | MS.gene036380:CDS |
| AATTTGATACAAATCTTAGC+AGG | 0.483019 | 2.4:-39357961 | None:intergenic |
| TAGAAAGGCAAGGTTGTTCA+AGG | 0.489258 | 2.4:+39358047 | MS.gene036380:CDS |
| TCATCATACCAGCCCTAGAA+AGG | 0.496553 | 2.4:+39358032 | MS.gene036380:CDS |
| ATTCTTGCATGCACAAGAGA+AGG | 0.499742 | 2.4:+39357877 | MS.gene036380:CDS |
| AAGATTAGAACATCGAGAAA+AGG | 0.502639 | 2.4:+39357829 | MS.gene036380:CDS |
| TCAAATGTTGTTGTGGATTG+TGG | 0.509257 | 2.4:+39357649 | MS.gene036380:CDS |
| CGACGTGCAATTGGAAAAGA+AGG | 0.512775 | 2.4:+39358231 | MS.gene036380:CDS |
| CTGGTATGATGAGAATGATC+AGG | 0.514347 | 2.4:-39358021 | None:intergenic |
| CATCGAGAAAAGGACGAGAT+GGG | 0.520186 | 2.4:+39357839 | MS.gene036380:CDS |
| AAGAAGATGATGATGCATGC+TGG | 0.530084 | 2.4:+39357719 | MS.gene036380:CDS |
| GATGGTGAAGGTATAGAAGT+TGG | 0.532672 | 2.4:+39357679 | MS.gene036380:CDS |
| ACATCGAGAAAAGGACGAGA+TGG | 0.535829 | 2.4:+39357838 | MS.gene036380:CDS |
| GGATAGTTCAAATGTTGTTG+TGG | 0.550233 | 2.4:+39357642 | MS.gene036380:CDS |
| GTGGATTGTGGTAGTACTGA+TGG | 0.559597 | 2.4:+39357661 | MS.gene036380:CDS |
| ATTGAAATCAATGATGATGC+AGG | 0.561814 | 2.4:+39358105 | MS.gene036380:CDS |
| TTCTTGCATGCACAAGAGAA+GGG | 0.575717 | 2.4:+39357878 | MS.gene036380:CDS |
| ATTGAAGACACGTCCGACAA+TGG | 0.579883 | 2.4:+39357927 | MS.gene036380:CDS |
| AGAAGATGATGATGCATGCT+GGG | 0.582547 | 2.4:+39357720 | MS.gene036380:CDS |
| ATCGAGAAAAGGACGAGATG+GGG | 0.592032 | 2.4:+39357840 | MS.gene036380:CDS |
| AATTGAAAGAAGATGGATTG+TGG | 0.618386 | 2.4:+39357980 | MS.gene036380:CDS |
| GAGAAAAGGACGAGATGGGG+AGG | 0.628028 | 2.4:+39357843 | MS.gene036380:CDS |
| CAATGATGATGCAGGTGTGA+GGG | 0.635539 | 2.4:+39358113 | MS.gene036380:CDS |
| AAATGGACTTGCATGTAAGG+AGG | 0.643265 | 2.4:+39358079 | MS.gene036380:CDS |
| CTCTGGTTAAAGATGCAGGA+GGG | 0.643359 | 2.4:+39358154 | MS.gene036380:CDS |
| TGTGGTAGTACTGATGGTGA+AGG | 0.646539 | 2.4:+39357667 | MS.gene036380:CDS |
| TGTGAGGAACTATGTTAACA+AGG | 0.653364 | 2.4:+39358206 | MS.gene036380:CDS |
| TCAATGATGATGCAGGTGTG+AGG | 0.662085 | 2.4:+39358112 | MS.gene036380:CDS |
| ATACCAGCCCTAGAAAGGCA+AGG | 0.674438 | 2.4:+39358037 | MS.gene036380:CDS |
| TGCATGCAAGAATTAAGTAG+CGG | 0.685653 | 2.4:-39357867 | None:intergenic |
| CATTTAATGAAAGAGATGTG+AGG | 0.706266 | 2.4:+39358190 | MS.gene036380:CDS |
| TCTCTGGTTAAAGATGCAGG+AGG | 0.725630 | 2.4:+39358153 | MS.gene036380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAAAACACATATCTATTTT+AGG | - | chr2.4:39357748-39357767 | None:intergenic | 20.0% |
| !!! | TTTTGTTTGTTCAAATTTGT+AGG | + | chr2.4:39358391-39358410 | MS.gene036380:intron | 20.0% |
| ! | AATTTGATACAAATCTTAGC+AGG | - | chr2.4:39357964-39357983 | None:intergenic | 25.0% |
| ! | TAATTTCTTATATGCGTAGA+GGG | + | chr2.4:39358305-39358324 | MS.gene036380:intron | 25.0% |
| ! | TGTATCAAATTGAAAGAAGA+TGG | + | chr2.4:39357973-39357992 | MS.gene036380:CDS | 25.0% |
| ! | TTGACAAAAATAGCTTATCA+AGG | - | chr2.4:39358268-39358287 | None:intergenic | 25.0% |
| ! | TTGTAATCACCATGTATATA+TGG | + | chr2.4:39358354-39358373 | MS.gene036380:intron | 25.0% |
| !!! | AAAGAGTATGCTTTTAGAAA+GGG | + | chr2.4:39357796-39357815 | MS.gene036380:CDS | 25.0% |
| !!! | AAGCTATTTTTGTCAAATGA+GGG | + | chr2.4:39358272-39358291 | MS.gene036380:intron | 25.0% |
| !!! | TAAAGAGTATGCTTTTAGAA+AGG | + | chr2.4:39357795-39357814 | MS.gene036380:CDS | 25.0% |
| !!! | TAAGCTATTTTTGTCAAATG+AGG | + | chr2.4:39358271-39358290 | MS.gene036380:intron | 25.0% |
| AAGATTAGAACATCGAGAAA+AGG | + | chr2.4:39357829-39357848 | MS.gene036380:CDS | 30.0% | |
| AGATGCAATCCATATATACA+TGG | - | chr2.4:39358366-39358385 | None:intergenic | 30.0% | |
| CATTTAATGAAAGAGATGTG+AGG | + | chr2.4:39358190-39358209 | MS.gene036380:CDS | 30.0% | |
| CTAATTTCTTATATGCGTAG+AGG | + | chr2.4:39358304-39358323 | MS.gene036380:intron | 30.0% | |
| CTCACATCTCTTTCATTAAA+TGG | - | chr2.4:39358192-39358211 | None:intergenic | 30.0% | |
| CTCTACGCATATAAGAAATT+AGG | - | chr2.4:39358306-39358325 | None:intergenic | 30.0% | |
| ! | ATTGAAATCAATGATGATGC+AGG | + | chr2.4:39358105-39358124 | MS.gene036380:CDS | 30.0% |
| !! | AATTGAAAGAAGATGGATTG+TGG | + | chr2.4:39357980-39357999 | MS.gene036380:CDS | 30.0% |
| !!! | CTTTTAGAAAGGGTTTTGAA+TGG | + | chr2.4:39357806-39357825 | MS.gene036380:CDS | 30.0% |
| GCAATTGGAAAAGAAGGTAA+TGG | + | chr2.4:39358237-39358256 | MS.gene036380:intron | 35.0% | |
| GGATAGTTCAAATGTTGTTG+TGG | + | chr2.4:39357642-39357661 | MS.gene036380:CDS | 35.0% | |
| TGCATGCAAGAATTAAGTAG+CGG | - | chr2.4:39357870-39357889 | None:intergenic | 35.0% | |
| TGTGAGGAACTATGTTAACA+AGG | + | chr2.4:39358206-39358225 | MS.gene036380:CDS | 35.0% | |
| ! | AGAAAATGGACTTGCATGTA+AGG | + | chr2.4:39358076-39358095 | MS.gene036380:CDS | 35.0% |
| ! | ATGCAAGTCCATTTTCTTGT+TGG | - | chr2.4:39358073-39358092 | None:intergenic | 35.0% |
| ! | CAACAAGACTTTTCAATCTC+TGG | + | chr2.4:39358137-39358156 | MS.gene036380:CDS | 35.0% |
| ! | TCAAATGTTGTTGTGGATTG+TGG | + | chr2.4:39357649-39357668 | MS.gene036380:CDS | 35.0% |
| !!! | GGACAATTTTTTCCCATTGT+CGG | - | chr2.4:39357943-39357962 | None:intergenic | 35.0% |
| ATTCTTGCATGCACAAGAGA+AGG | + | chr2.4:39357877-39357896 | MS.gene036380:CDS | 40.0% | |
| CAATCTCTGGTTAAAGATGC+AGG | + | chr2.4:39358150-39358169 | MS.gene036380:CDS | 40.0% | |
| CTGGTATGATGAGAATGATC+AGG | - | chr2.4:39358024-39358043 | None:intergenic | 40.0% | |
| GTTCAAGGCCAACAAGAAAA+TGG | + | chr2.4:39358062-39358081 | MS.gene036380:CDS | 40.0% | |
| TAGAAAGGCAAGGTTGTTCA+AGG | + | chr2.4:39358047-39358066 | MS.gene036380:CDS | 40.0% | |
| TGAACAACCTTGCCTTTCTA+GGG | - | chr2.4:39358047-39358066 | None:intergenic | 40.0% | |
| TTCTTGCATGCACAAGAGAA+GGG | + | chr2.4:39357878-39357897 | MS.gene036380:CDS | 40.0% | |
| TTGAACAACCTTGCCTTTCT+AGG | - | chr2.4:39358048-39358067 | None:intergenic | 40.0% | |
| ! | AAATGGACTTGCATGTAAGG+AGG | + | chr2.4:39358079-39358098 | MS.gene036380:CDS | 40.0% |
| ! | AAGAAGATGATGATGCATGC+TGG | + | chr2.4:39357719-39357738 | MS.gene036380:CDS | 40.0% |
| ! | AGAAGATGATGATGCATGCT+GGG | + | chr2.4:39357720-39357739 | MS.gene036380:CDS | 40.0% |
| ! | GATGGTGAAGGTATAGAAGT+TGG | + | chr2.4:39357679-39357698 | MS.gene036380:CDS | 40.0% |
| ! | TGGAAAAGAAGGTAATGGCA+AGG | + | chr2.4:39358242-39358261 | MS.gene036380:intron | 40.0% |
| AACAAGGAACGACGTGCAAT+TGG | + | chr2.4:39358222-39358241 | MS.gene036380:CDS | 45.0% | |
| ACATCGAGAAAAGGACGAGA+TGG | + | chr2.4:39357838-39357857 | MS.gene036380:CDS | 45.0% | |
| ATCGAGAAAAGGACGAGATG+GGG | + | chr2.4:39357840-39357859 | MS.gene036380:CDS | 45.0% | |
| ATTGAAGACACGTCCGACAA+TGG | + | chr2.4:39357927-39357946 | MS.gene036380:CDS | 45.0% | |
| CATCGAGAAAAGGACGAGAT+GGG | + | chr2.4:39357839-39357858 | MS.gene036380:CDS | 45.0% | |
| CGACGTGCAATTGGAAAAGA+AGG | + | chr2.4:39358231-39358250 | MS.gene036380:CDS | 45.0% | |
| CTCTGGTTAAAGATGCAGGA+GGG | + | chr2.4:39358154-39358173 | MS.gene036380:CDS | 45.0% | |
| TCATCATACCAGCCCTAGAA+AGG | + | chr2.4:39358032-39358051 | MS.gene036380:CDS | 45.0% | |
| TCTCTGGTTAAAGATGCAGG+AGG | + | chr2.4:39358153-39358172 | MS.gene036380:CDS | 45.0% | |
| TTGAAGACACGTCCGACAAT+GGG | + | chr2.4:39357928-39357947 | MS.gene036380:CDS | 45.0% | |
| ! | CAATGATGATGCAGGTGTGA+GGG | + | chr2.4:39358113-39358132 | MS.gene036380:CDS | 45.0% |
| ! | GTGGATTGTGGTAGTACTGA+TGG | + | chr2.4:39357661-39357680 | MS.gene036380:CDS | 45.0% |
| ! | TCAATGATGATGCAGGTGTG+AGG | + | chr2.4:39358112-39358131 | MS.gene036380:CDS | 45.0% |
| !! | TGTGGTAGTACTGATGGTGA+AGG | + | chr2.4:39357667-39357686 | MS.gene036380:CDS | 45.0% |
| ATACCAGCCCTAGAAAGGCA+AGG | + | chr2.4:39358037-39358056 | MS.gene036380:CDS | 50.0% | |
| GAGAAAAGGACGAGATGGGG+AGG | + | chr2.4:39357843-39357862 | MS.gene036380:CDS | 55.0% | |
| !! | CAACCTTGCCTTTCTAGGGC+TGG | - | chr2.4:39358043-39358062 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 39357640 | 39358426 | 39357640 | ID=MS.gene036380 |
| chr2.4 | mRNA | 39357640 | 39358426 | 39357640 | ID=MS.gene036380.t1;Parent=MS.gene036380 |
| chr2.4 | exon | 39357640 | 39358252 | 39357640 | ID=MS.gene036380.t1.exon1;Parent=MS.gene036380.t1 |
| chr2.4 | CDS | 39357640 | 39358252 | 39357640 | ID=cds.MS.gene036380.t1;Parent=MS.gene036380.t1 |
| chr2.4 | exon | 39358413 | 39358426 | 39358413 | ID=MS.gene036380.t1.exon2;Parent=MS.gene036380.t1 |
| chr2.4 | CDS | 39358413 | 39358426 | 39358413 | ID=cds.MS.gene036380.t1;Parent=MS.gene036380.t1 |
| Gene Sequence |
| Protein sequence |
