AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036427

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036427.t1 MTR_1g080330 98.196 388 7 0 1 388 1 388 0.0 779
MS.gene036427.t1 MTR_1g080330 98.936 282 3 0 1 282 1 282 0.0 576
MS.gene036427.t1 MTR_7g115530 54.415 419 134 11 8 385 37 439 1.60e-128 377
MS.gene036427.t1 MTR_7g115530 57.516 306 84 8 8 283 37 326 1.45e-98 296
MS.gene036427.t1 MTR_7g098250 44.893 421 176 11 21 388 72 489 2.01e-96 296
MS.gene036427.t1 MTR_7g115530 54.804 281 81 8 8 258 37 301 2.85e-81 251
MS.gene036427.t1 MTR_7g115530 54.717 265 74 8 8 242 37 285 2.74e-74 235
MS.gene036427.t1 MTR_7g115530 51.969 254 76 8 8 231 37 274 4.58e-64 206
MS.gene036427.t1 MTR_7g115530 51.200 250 76 7 8 227 37 270 1.64e-62 202
MS.gene036427.t1 MTR_7g093030 58.955 134 54 1 174 307 211 343 4.00e-51 176
MS.gene036427.t1 MTR_1g053835 55.488 164 59 5 166 329 253 402 4.41e-50 174
MS.gene036427.t1 MTR_2g027860 55.921 152 63 2 175 322 49 200 7.16e-47 163
MS.gene036427.t1 MTR_7g088070 53.289 152 70 1 174 325 49 199 6.75e-46 159
MS.gene036427.t1 MTR_7g088070 52.903 155 70 2 174 325 49 203 1.10e-44 156
MS.gene036427.t1 MTR_6g032990 45.026 191 64 5 171 327 38 221 1.65e-40 148
MS.gene036427.t1 MTR_2g086450 43.558 163 72 2 161 303 31 193 1.49e-36 137
MS.gene036427.t1 MTR_5g027440 42.857 182 81 5 168 326 18 199 1.67e-34 129
MS.gene036427.t1 MTR_5g041350 40.625 160 77 3 171 314 11 168 5.89e-34 129
MS.gene036427.t1 MTR_1g053800 46.479 142 75 1 165 306 167 307 9.14e-34 128
MS.gene036427.t1 MTR_4g081710 41.176 170 78 4 171 325 13 175 1.37e-33 128
MS.gene036427.t1 MTR_6g444980 40.351 171 93 3 165 332 25 189 4.22e-33 127
MS.gene036427.t1 MTR_6g444980 45.985 137 71 2 165 298 25 161 2.46e-32 125
MS.gene036427.t1 MTR_7g093030 60.000 85 33 1 174 258 211 294 1.71e-30 118
MS.gene036427.t1 MTR_7g068600 40.741 162 90 4 175 331 13 173 3.02e-30 116
MS.gene036427.t1 MTR_2g027800 45.652 138 65 3 167 300 31 162 4.51e-29 115
MS.gene036427.t1 MTR_2g027800 40.411 146 75 2 167 300 31 176 2.16e-28 113
MS.gene036427.t1 MTR_1g090670 40.559 143 76 2 167 300 36 178 3.62e-28 112
MS.gene036427.t1 MTR_1g053830 52.273 88 40 2 164 251 8 93 6.25e-25 97.8
MS.gene036427.t1 MTR_7g093010 42.636 129 71 2 174 300 212 339 5.36e-23 99.4
MS.gene036427.t1 MTR_0223s0040 69.643 56 17 0 176 231 19 74 3.94e-20 89.7
MS.gene036427.t1 MTR_2g023580 66.071 56 19 0 176 231 56 111 7.09e-20 88.2
MS.gene036427.t1 MTR_1g112370 67.857 56 18 0 176 231 23 78 8.11e-20 89.7
MS.gene036427.t1 MTR_3g450310 59.649 57 23 0 175 231 19 75 1.56e-19 86.7
MS.gene036427.t1 MTR_3g064840 43.860 114 48 3 143 254 254 353 1.58e-19 90.5
MS.gene036427.t1 MTR_7g069660 59.701 67 26 1 166 231 61 127 7.89e-19 87.4
MS.gene036427.t1 MTR_2g016220 56.579 76 32 1 157 231 70 145 8.40e-19 87.4
MS.gene036427.t1 MTR_2g016220 56.579 76 32 1 157 231 70 145 8.46e-19 87.4
MS.gene036427.t1 MTR_2g084230 61.194 67 25 1 166 231 87 153 1.17e-18 86.3
MS.gene036427.t1 MTR_2g084230 61.194 67 25 1 166 231 87 153 1.58e-18 86.7
MS.gene036427.t1 MTR_8g086410 64.286 56 20 0 176 231 110 165 2.81e-18 86.3
MS.gene036427.t1 MTR_8g086410 64.286 56 20 0 176 231 112 167 2.84e-18 86.3
MS.gene036427.t1 MTR_1g021520 58.824 68 28 0 172 239 123 190 4.58e-18 84.3
MS.gene036427.t1 MTR_8g066310 45.283 106 43 3 136 239 147 239 5.74e-18 84.7
MS.gene036427.t1 MTR_6g071625 62.500 64 22 1 176 237 203 266 1.56e-17 83.6
MS.gene036427.t1 MTR_8g086410 64.815 54 19 0 176 229 112 165 3.52e-17 83.2
MS.gene036427.t1 MTR_5g029470 63.333 60 22 0 172 231 216 275 4.66e-17 82.4
MS.gene036427.t1 MTR_2g016220 58.571 70 28 1 157 225 70 139 6.65e-17 82.0
MS.gene036427.t1 MTR_4g113140 48.235 85 42 1 166 248 226 310 3.56e-15 77.0
MS.gene036427.t1 MTR_2g450070 58.621 58 23 1 171 228 209 265 1.82e-14 75.5
MS.gene036427.t1 MTR_2g450070 58.621 58 23 1 171 228 209 265 1.84e-14 75.5
MS.gene036427.t1 MTR_4g021790 50.000 76 36 2 171 246 164 237 3.64e-14 72.4
MS.gene036427.t1 MTR_4g086835 56.250 64 27 1 175 237 274 337 1.99e-13 71.6
MS.gene036427.t1 MTR_4g021855 41.748 103 50 4 164 258 158 258 2.63e-13 70.5
MS.gene036427.t1 MTR_1g013170 52.941 68 31 1 171 238 187 253 5.70e-13 70.5
MS.gene036427.t1 MTR_3g106220 56.897 58 24 1 171 228 194 250 6.68e-13 70.5
MS.gene036427.t1 MTR_4g131600 54.237 59 26 1 170 228 196 253 6.90e-13 70.5
MS.gene036427.t1 MTR_5g054300 58.929 56 23 0 173 228 196 251 7.97e-13 69.3
MS.gene036427.t1 MTR_4g121020 43.478 92 43 2 175 258 111 201 8.58e-13 70.1
MS.gene036427.t1 MTR_5g017980 53.731 67 30 1 172 237 190 256 8.64e-13 68.9
MS.gene036427.t1 MTR_4g121020 41.237 97 48 2 170 258 210 305 9.92e-13 70.1
MS.gene036427.t1 MTR_4g121020 43.478 92 43 2 175 258 215 305 1.00e-12 70.1
MS.gene036427.t1 MTR_1g093080 49.231 65 33 0 173 237 209 273 1.05e-12 69.3
MS.gene036427.t1 MTR_4g098870 46.739 92 30 3 153 228 294 382 1.29e-12 69.7
MS.gene036427.t1 MTR_4g098870 46.739 92 30 3 153 228 274 362 1.36e-12 69.3
MS.gene036427.t1 MTR_4g098870 46.739 92 30 3 153 228 294 382 1.73e-12 69.3
MS.gene036427.t1 MTR_4g098870 46.739 92 30 3 153 228 313 401 1.92e-12 68.9
MS.gene036427.t1 MTR_4g098870 46.739 92 30 3 153 228 274 362 1.97e-12 68.9
MS.gene036427.t1 MTR_4g064730 50.000 72 34 2 158 228 113 183 1.99e-12 67.8
MS.gene036427.t1 MTR_4g131570 50.000 64 31 1 171 234 199 261 3.06e-12 68.6
MS.gene036427.t1 MTR_4g086835 56.250 64 26 2 175 237 274 336 4.81e-12 67.4
MS.gene036427.t1 MTR_1g032570 52.941 68 25 2 161 228 192 252 5.39e-12 67.8
MS.gene036427.t1 MTR_1g032570 52.941 68 25 2 161 228 123 183 5.72e-12 67.4
MS.gene036427.t1 MTR_4g131580 51.562 64 30 1 171 234 196 258 6.27e-12 67.4
MS.gene036427.t1 MTR_1g013180 55.932 59 25 1 170 228 337 394 8.14e-12 67.4
MS.gene036427.t1 MTR_7g089010 55.556 54 23 1 175 228 91 143 1.31e-11 65.5
MS.gene036427.t1 MTR_5g017980 53.731 67 29 2 172 237 190 255 2.10e-11 65.1
MS.gene036427.t1 MTR_5g017980 53.731 67 29 2 172 237 189 254 2.10e-11 65.1
MS.gene036427.t1 MTR_7g117705 55.172 58 25 1 171 228 187 243 2.67e-11 65.5
MS.gene036427.t1 MTR_2g034960 50.847 59 28 1 170 228 206 263 4.68e-11 64.7
MS.gene036427.t1 MTR_4g078810 49.153 59 29 1 173 231 156 213 4.92e-11 64.3
MS.gene036427.t1 MTR_5g014040 53.448 58 26 1 171 228 307 363 7.71e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036427.t1 AT5G29000 56.972 251 99 6 61 306 76 322 1.91e-83 259
MS.gene036427.t1 AT5G29000 56.972 251 99 6 61 306 76 322 1.91e-83 259
MS.gene036427.t1 AT5G29000 56.972 251 99 6 61 306 119 365 4.98e-83 259
MS.gene036427.t1 AT3G04450 50.909 275 117 7 43 309 71 335 8.14e-78 246
MS.gene036427.t1 AT3G04450 50.909 275 117 7 43 309 71 335 8.14e-78 246
MS.gene036427.t1 AT3G04450 50.909 275 117 7 43 309 111 375 1.54e-77 246
MS.gene036427.t1 AT4G28610 49.346 306 130 9 17 304 57 355 3.20e-77 244
MS.gene036427.t1 AT2G20400 48.889 225 96 4 93 304 142 360 3.51e-55 187
MS.gene036427.t1 AT2G20400 48.673 226 96 5 93 304 142 361 2.01e-53 182
MS.gene036427.t1 AT2G01060 58.125 160 58 3 161 315 5 160 2.29e-51 174
MS.gene036427.t1 AT5G29000 52.778 180 79 4 61 238 119 294 3.13e-49 169
MS.gene036427.t1 AT5G06800 57.343 143 60 1 167 309 184 325 3.21e-49 171
MS.gene036427.t1 AT3G04450 47.761 201 90 6 43 238 111 301 4.38e-48 166
MS.gene036427.t1 AT5G06800 55.405 148 60 2 167 309 184 330 5.80e-47 165
MS.gene036427.t1 AT3G13040 56.643 143 57 1 170 307 236 378 6.48e-43 155
MS.gene036427.t1 AT3G13040 56.643 143 57 1 170 307 236 378 6.48e-43 155
MS.gene036427.t1 AT3G13040 56.643 143 57 1 170 307 236 378 6.48e-43 155
MS.gene036427.t1 AT4G13640 42.105 209 96 6 161 356 22 218 6.99e-42 149
MS.gene036427.t1 AT3G24120 45.882 170 78 4 162 317 27 196 3.76e-40 144
MS.gene036427.t1 AT4G13640 41.509 212 96 7 161 356 22 221 7.11e-40 144
MS.gene036427.t1 AT3G24120 45.087 173 78 5 162 317 27 199 4.87e-38 139
MS.gene036427.t1 AT3G04030 44.304 158 65 2 165 303 35 188 6.97e-38 141
MS.gene036427.t1 AT5G18240 45.122 164 63 3 161 303 31 188 2.79e-37 139
MS.gene036427.t1 AT5G18240 45.122 164 63 3 161 303 31 188 2.79e-37 139
MS.gene036427.t1 AT1G69580 44.311 167 73 2 167 316 26 189 4.01e-37 137
MS.gene036427.t1 AT1G69580 44.706 170 69 5 167 316 26 190 1.07e-36 136
MS.gene036427.t1 AT3G04030 43.038 158 66 3 165 303 35 187 8.18e-35 132
MS.gene036427.t1 AT1G79430 44.295 149 74 3 165 307 24 169 2.87e-34 130
MS.gene036427.t1 AT5G18240 44.512 164 62 4 161 303 31 186 2.93e-34 131
MS.gene036427.t1 AT5G45580 40.373 161 78 1 165 307 13 173 3.94e-33 125
MS.gene036427.t1 AT3G04030 41.772 158 63 3 165 303 35 182 3.73e-32 125
MS.gene036427.t1 AT5G45580 40.645 155 74 1 171 307 3 157 4.80e-32 122
MS.gene036427.t1 AT5G18240 42.683 164 61 4 161 303 31 182 1.49e-31 124
MS.gene036427.t1 AT5G18240 42.683 164 61 4 161 303 31 182 1.49e-31 124
MS.gene036427.t1 AT2G01060 56.757 111 43 2 210 315 1 111 7.85e-31 118
MS.gene036427.t1 AT3G12730 39.752 161 89 4 159 311 7 167 4.83e-30 115
MS.gene036427.t1 AT5G06800 61.644 73 28 0 167 239 184 256 3.18e-25 103
MS.gene036427.t1 AT1G14600 69.643 56 17 0 176 231 24 79 8.76e-20 88.6
MS.gene036427.t1 AT2G42660 56.923 65 28 0 176 240 51 115 1.19e-19 88.2
MS.gene036427.t1 AT4G04605 49.425 87 43 1 163 248 1 87 4.00e-19 85.9
MS.gene036427.t1 AT5G42630 60.938 64 25 0 176 239 106 169 2.26e-18 83.6
MS.gene036427.t1 AT2G02060 67.925 53 17 0 176 228 30 82 3.20e-18 84.0
MS.gene036427.t1 AT2G02060 67.925 53 17 0 176 228 30 82 3.20e-18 84.0
MS.gene036427.t1 AT1G32240 47.674 86 33 1 146 231 196 269 3.26e-18 85.9
MS.gene036427.t1 AT1G32240 47.674 86 33 1 146 231 196 269 3.68e-18 85.9
MS.gene036427.t1 AT2G40260 60.606 66 25 1 167 231 73 138 3.93e-18 85.9
MS.gene036427.t1 AT5G16560 46.535 101 39 3 148 245 204 292 4.40e-18 85.5
MS.gene036427.t1 AT5G42630 60.938 64 25 0 176 239 106 169 4.93e-18 84.0
MS.gene036427.t1 AT4G17695 62.121 66 23 1 176 239 165 230 5.42e-18 84.3
MS.gene036427.t1 AT3G04030 39.655 116 47 2 207 303 1 112 2.15e-17 82.8
MS.gene036427.t1 AT2G06020 52.308 65 31 0 167 231 78 142 3.87e-17 81.6
MS.gene036427.t1 AT2G38300 60.714 56 22 0 176 231 55 110 4.25e-17 82.0
MS.gene036427.t1 AT1G79430 38.318 107 57 3 207 307 1 104 9.40e-16 77.4
MS.gene036427.t1 AT4G04580 55.385 65 28 1 176 240 15 78 1.56e-15 74.3
MS.gene036427.t1 AT2G03500 57.746 71 28 1 169 237 225 295 1.85e-15 78.2
MS.gene036427.t1 AT1G25550 57.143 63 27 0 172 234 206 268 7.60e-15 75.5
MS.gene036427.t1 AT4G37180 52.308 65 31 0 164 228 199 263 7.68e-14 72.8
MS.gene036427.t1 AT4G37180 52.308 65 31 0 164 228 206 270 8.31e-14 72.4
MS.gene036427.t1 AT1G68670 51.515 66 32 0 172 237 212 277 1.43e-13 71.6
MS.gene036427.t1 AT4G31920 48.101 79 39 2 171 249 179 255 1.72e-13 72.4
MS.gene036427.t1 AT3G16857 64.815 54 18 1 175 228 167 219 6.02e-13 70.9
MS.gene036427.t1 AT3G16857 64.815 54 18 1 175 228 237 289 6.46e-13 70.9
MS.gene036427.t1 AT3G16857 64.815 54 18 1 175 228 237 289 6.47e-13 70.9
MS.gene036427.t1 AT5G58080 57.895 57 23 1 172 228 191 246 9.06e-13 70.1
MS.gene036427.t1 AT4G04555 47.143 70 36 1 169 238 8 76 9.53e-13 64.7
MS.gene036427.t1 AT2G25180 59.322 59 23 1 170 228 190 247 1.45e-12 69.7
MS.gene036427.t1 AT5G44190 54.237 59 26 1 173 231 145 202 1.46e-12 68.9
MS.gene036427.t1 AT5G44190 54.237 59 26 1 173 231 145 202 1.79e-12 68.6
MS.gene036427.t1 AT1G49560 55.556 63 28 0 175 237 192 254 2.45e-12 67.8
MS.gene036427.t1 AT1G25550 59.259 54 22 0 172 225 206 259 2.62e-12 67.0
MS.gene036427.t1 AT2G40970 57.407 54 22 1 175 228 105 157 2.71e-12 66.6
MS.gene036427.t1 AT5G05090 55.556 54 23 1 175 228 81 133 3.69e-12 66.6
MS.gene036427.t1 AT3G62670 53.968 63 25 1 175 233 211 273 4.46e-12 67.8
MS.gene036427.t1 AT3G10760 55.556 54 23 1 175 228 105 157 4.94e-12 67.0
MS.gene036427.t1 AT5G59570 50.820 61 29 1 168 228 134 193 5.23e-12 66.6
MS.gene036427.t1 AT5G59570 50.820 61 29 1 168 228 134 193 5.23e-12 66.6
MS.gene036427.t1 AT3G25790 54.545 55 25 0 180 234 201 255 6.13e-12 67.0
MS.gene036427.t1 AT3G46640 45.833 72 38 1 157 228 126 196 6.75e-12 66.6
MS.gene036427.t1 AT3G46640 45.833 72 38 1 157 228 126 196 7.08e-12 66.2
MS.gene036427.t1 AT3G46640 45.833 72 38 1 157 228 126 196 7.08e-12 66.2
MS.gene036427.t1 AT2G20570 55.932 59 25 1 173 231 151 208 1.13e-11 66.2
MS.gene036427.t1 AT4G37180 50.000 62 31 0 164 225 199 260 1.32e-11 65.1
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.83e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.83e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.83e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.83e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.83e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.83e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.94e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.94e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.94e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.94e-11 65.9
MS.gene036427.t1 AT4G18020 50.769 65 31 1 167 231 288 351 1.94e-11 65.9
MS.gene036427.t1 AT2G20570 40.000 90 48 2 177 261 171 259 2.02e-11 65.5
MS.gene036427.t1 AT1G49190 52.632 57 24 1 175 228 215 271 2.99e-11 65.1
MS.gene036427.t1 AT1G13300 47.619 63 33 0 175 237 181 243 7.06e-11 63.5
MS.gene036427.t1 AT1G67710 57.692 52 22 0 177 228 121 172 9.57e-11 63.5

Find 94 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCTCTGTGCTTGTATTC+TGG 0.101134 1.4:+65288036 None:intergenic
TGTATTCCACATGGTCATTC+TGG 0.149403 1.4:+65290335 None:intergenic
AGGAAGATCATTAACTTCTT+TGG 0.221222 1.4:+65289651 None:intergenic
CACATACCTTCTGATGATTC+TGG 0.228637 1.4:+65289248 None:intergenic
TTGCTCTGTGCTTGTATTCT+GGG 0.237413 1.4:+65288037 None:intergenic
CCGAGAAGTTCATTCCAATT+TGG 0.239899 1.4:+65290220 None:intergenic
ATGATTCTATTCAGCCAAAT+TGG 0.254038 1.4:-65290234 MS.gene036427:CDS
CTCCTTGAAATCCGTTGTTT+TGG 0.268176 1.4:+65290285 None:intergenic
GTTGTTCATGAAGCCGCTTC+TGG 0.275179 1.4:+65288409 None:intergenic
TCTGGAGTCCAACGCATTCT+AGG 0.277206 1.4:+65289595 None:intergenic
GTATTCTGGGAACTTCCTTC+AGG 0.358705 1.4:+65288050 None:intergenic
GAATACAAGCACAGAGCAAA+AGG 0.372831 1.4:-65288033 MS.gene036427:CDS
TTCTGGGTGGGAACATTATC+AGG 0.378603 1.4:+65290352 None:intergenic
ATTGAAGTCAACTTCTTCTC+TGG 0.390129 1.4:+65288798 None:intergenic
GTAGTCGACACAGAAGAATT+AGG 0.399816 1.4:+65289631 None:intergenic
GTATTCCACATGGTCATTCT+GGG 0.400152 1.4:+65290336 None:intergenic
TATACTGCTGCTGTGAAACC+TGG 0.426828 1.4:+65289689 None:intergenic
AGAACTTCATGAGGCCTTTG+TGG 0.428094 1.4:-65289575 MS.gene036427:CDS
AGCTACCGGTGAACTTGAGC+TGG 0.429993 1.4:-65287997 MS.gene036427:CDS
TGCCAAAACAACGGATTTCA+AGG 0.441739 1.4:-65290287 MS.gene036427:CDS
CCAAATTGGAATGAACTTCT+CGG 0.447252 1.4:-65290220 MS.gene036427:CDS
ACAAAGGCCTCATGAAGTTC+TGG 0.447758 1.4:+65289577 None:intergenic
TATTCCCTCTTCCTCGATGC+AGG 0.454247 1.4:-65290548 MS.gene036427:CDS
ATCAAGTTCAAGACACCCTT+AGG 0.470004 1.4:+65289431 None:intergenic
ATATGTAGAGGCTACTCCTA+AGG 0.470935 1.4:-65289447 MS.gene036427:intron
GATAGTGCCACACTTGATAG+TGG 0.493745 1.4:+65288152 None:intergenic
ACATAACGTCACTTTCTTGA+GGG 0.494013 1.4:+65290413 None:intergenic
TGATGATTCTGGTTTGTACC+TGG 0.500432 1.4:+65289259 None:intergenic
AGAAAGTGACGTTATGTCCT+GGG 0.500596 1.4:-65290407 MS.gene036427:CDS
AATTGAAGAATATCTTCAAA+TGG 0.505497 1.4:+65290376 None:intergenic
ATTGTGGTTGTATAGGCCTA+AGG 0.506694 1.4:-65288466 MS.gene036427:intron
TGCAGCCTGCATCGAGGAAG+AGG 0.509129 1.4:+65290543 None:intergenic
AGAAGCATTGCGGCTGCAGA+TGG 0.512926 1.4:-65288436 MS.gene036427:CDS
TACAATGGTTCTGAAGTCTT+GGG 0.514053 1.4:-65290316 MS.gene036427:CDS
AAGAAAGTGACGTTATGTCC+TGG 0.515386 1.4:-65290408 MS.gene036427:CDS
TCTCTTGATGCAAGAGACCC+AGG 0.517615 1.4:-65289707 MS.gene036427:CDS
AATTCCAACATCAGAAGTGT+TGG 0.526621 1.4:-65290514 MS.gene036427:CDS
ATACAATGGTTCTGAAGTCT+TGG 0.526812 1.4:-65290317 MS.gene036427:CDS
TTGAACTTGATGAAAGTTGA+GGG 0.529752 1.4:-65289419 MS.gene036427:CDS
TTGTGGGTGGTGCGGTAAGT+TGG 0.530504 1.4:+65287967 None:intergenic
CGTTATCAGGCTGTTCAGAA+GGG 0.533325 1.4:+65290476 None:intergenic
TTCTCCCAGCTCAAGTTCAC+CGG 0.534132 1.4:+65287992 None:intergenic
ACTTCTTTGGATGGTAGTGA+TGG 0.537531 1.4:+65289664 None:intergenic
GTCTCTTGCATCAAGAGAGA+AGG 0.539948 1.4:+65289712 None:intergenic
AATTTAGAAAACACAAATGA+AGG 0.541617 1.4:-65288116 MS.gene036427:CDS
TCATCTGTAGATGCTTCCCT+TGG 0.544034 1.4:+65288213 None:intergenic
TCATGTGAAAAGCCATCTGC+AGG 0.546156 1.4:-65289384 MS.gene036427:intron
ACGTTATCAGGCTGTTCAGA+AGG 0.551148 1.4:+65290475 None:intergenic
TTGTGGTTGTATAGGCCTAA+GGG 0.553041 1.4:-65288465 MS.gene036427:intron
TCTCTTGCATCAAGAGAGAA+GGG 0.556360 1.4:+65289713 None:intergenic
AGATCATTAACTTCTTTGGA+TGG 0.562377 1.4:+65289655 None:intergenic
TTCAAATGGATCAGTTCCCC+AGG 0.563936 1.4:+65290390 None:intergenic
AATGCTTCTGTAATCCCCTT+AGG 0.564243 1.4:+65288450 None:intergenic
AATACAAGCACAGAGCAAAA+GGG 0.565422 1.4:-65288032 MS.gene036427:CDS
TATGTAGAGGCTACTCCTAA+GGG 0.566576 1.4:-65289446 MS.gene036427:intron
CTGATTGACTGCTTCCACAA+AGG 0.570201 1.4:+65289561 None:intergenic
GACATAACGTCACTTTCTTG+AGG 0.571867 1.4:+65290412 None:intergenic
TTCAGAAATATAGAACTGCC+AGG 0.576431 1.4:-65289277 MS.gene036427:intron
GGAGATGATGCTAAAGCTAC+CGG 0.580790 1.4:-65288011 MS.gene036427:CDS
AATCTAAGCCTAGAATGCGT+TGG 0.584166 1.4:-65289603 MS.gene036427:CDS
AGATTCAGATTGAGAACCAA+GGG 0.585597 1.4:-65288229 MS.gene036427:CDS
GTGGAAGCAGTCAATCAGCT+TGG 0.585863 1.4:-65289556 MS.gene036427:CDS
AACAACGGATTTCAAGGAGT+GGG 0.590584 1.4:-65290281 MS.gene036427:CDS
GAAGCAGTCAATCAGCTTGG+TGG 0.592276 1.4:-65289553 MS.gene036427:CDS
AATGGTTCTGAAGTCTTGGG+TGG 0.595066 1.4:-65290313 MS.gene036427:CDS
ACACTTGATAGTGGAGCAGA+AGG 0.602869 1.4:+65288161 None:intergenic
TATGACAGAACCAAAATCAC+AGG 0.603724 1.4:-65290188 MS.gene036427:intron
CTTGAACTTGATGAAAGTTG+AGG 0.606827 1.4:-65289420 MS.gene036427:CDS
GTGTCCAACACTTCTGATGT+TGG 0.607725 1.4:+65290510 None:intergenic
ATACTGCTGCTGTGAAACCT+GGG 0.609600 1.4:+65289690 None:intergenic
GCGGCTTCATGAACAACTTG+AGG 0.611704 1.4:-65288403 MS.gene036427:intron
CAGATTCAGATTGAGAACCA+AGG 0.612607 1.4:-65288230 MS.gene036427:CDS
AGAATGTATTTCTGAAGCAG+AGG 0.614653 1.4:+65290444 None:intergenic
TTCCACATGGTCATTCTGGG+TGG 0.615350 1.4:+65290339 None:intergenic
GCTACCGGTGAACTTGAGCT+GGG 0.615664 1.4:-65287996 MS.gene036427:CDS
TCCACATGGTCATTCTGGGT+GGG 0.616235 1.4:+65290340 None:intergenic
GCAGCCTGCATCGAGGAAGA+GGG 0.625876 1.4:+65290544 None:intergenic
AAACAACGGATTTCAAGGAG+TGG 0.628215 1.4:-65290282 MS.gene036427:CDS
GTTATCAGGCTGTTCAGAAG+GGG 0.629071 1.4:+65290477 None:intergenic
ACTTCAGACAACATGCCTGA+AGG 0.630642 1.4:-65288065 MS.gene036427:CDS
CAGAACCATTGTATTCCACA+TGG 0.632141 1.4:+65290326 None:intergenic
TGCAGATGGAACTCCAGAAG+CGG 0.633918 1.4:-65288422 MS.gene036427:CDS
TACAAACCAGAATCATCAGA+AGG 0.635144 1.4:-65289254 MS.gene036427:intron
TGTGGTTGTATAGGCCTAAG+GGG 0.641482 1.4:-65288464 MS.gene036427:intron
TTCTGCTCCACTATCAAGTG+TGG 0.642875 1.4:-65288159 MS.gene036427:CDS
AATGACCATGTGGAATACAA+TGG 0.649000 1.4:-65290331 MS.gene036427:CDS
TTGGACTCCAGAACTTCATG+AGG 0.657310 1.4:-65289584 MS.gene036427:CDS
GAAAGTGACGTTATGTCCTG+GGG 0.673471 1.4:-65290406 MS.gene036427:CDS
GGGTGGAAATGCCAAAACAA+CGG 0.682227 1.4:-65290296 MS.gene036427:CDS
GTTTGAGCAACAGATGAAGT+CGG 0.685798 1.4:-65288189 MS.gene036427:CDS
CTCTTGCATCAAGAGAGAAG+GGG 0.697249 1.4:+65289714 None:intergenic
AGGGGATTACAGAAGCATTG+CGG 0.709657 1.4:-65288446 MS.gene036427:CDS
AATTGATGCAGCCTGCATCG+AGG 0.729819 1.4:+65290537 None:intergenic
TCCCACCCAGAATGACCATG+TGG 0.730540 1.4:-65290341 MS.gene036427:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACAATTATAGATAAGT+AGG + chr1.4:65289774-65289793 None:intergenic 15.0%
!! AAAACAATTATAGATAAGTA+GGG + chr1.4:65289773-65289792 None:intergenic 15.0%
!!! CATTTTTTTTTTCTTTACTT+AGG - chr1.4:65289144-65289163 MS.gene036427:intron 15.0%
!!! TTCTATATTTCTGAAATTAA+GGG + chr1.4:65289221-65289240 None:intergenic 15.0%
!! AAACAATTATAGATAAGTAG+GGG + chr1.4:65289772-65289791 None:intergenic 20.0%
!! AATTGAAGAATATCTTCAAA+TGG + chr1.4:65288130-65288149 None:intergenic 20.0%
!! AATTTAGAAAACACAAATGA+AGG - chr1.4:65290387-65290406 MS.gene036427:CDS 20.0%
!! ACATTAATAACTGTACATAT+TGG + chr1.4:65290002-65290021 None:intergenic 20.0%
!! ATATAAAGTTCATATAAACC+AGG - chr1.4:65289304-65289323 MS.gene036427:intron 20.0%
!! TTAATAAGCACATACATAAT+TGG - chr1.4:65289607-65289626 MS.gene036427:CDS 20.0%
!! TTGCTTATTATTTATATCTG+TGG - chr1.4:65289327-65289346 MS.gene036427:intron 20.0%
!!! GATTTTAATATTGTACTACA+AGG - chr1.4:65288354-65288373 MS.gene036427:intron 20.0%
!!! GTTCTATATTTCTGAAATTA+AGG + chr1.4:65289222-65289241 None:intergenic 20.0%
!!! TTTCTTTTTATAGGCAAATA+CGG - chr1.4:65289416-65289435 MS.gene036427:CDS 20.0%
! ATAATACTAGAATATGTGGA+TGG - chr1.4:65288404-65288423 MS.gene036427:CDS 25.0%
! ATTAACACCATGAGATAATA+AGG + chr1.4:65289038-65289057 None:intergenic 25.0%
! GATATAAATAATAAGCAACC+TGG + chr1.4:65289325-65289344 None:intergenic 25.0%
! GTGAATAATACTAGAATATG+TGG - chr1.4:65288400-65288419 MS.gene036427:intron 25.0%
! TCATCTTCCTTACAAAATAT+GGG + chr1.4:65290194-65290213 None:intergenic 25.0%
! TTCTAGTATTATTCACAGAT+AGG + chr1.4:65288396-65288415 None:intergenic 25.0%
! TTGATTCATCCAAAACAAAT+TGG + chr1.4:65289640-65289659 None:intergenic 25.0%
!! AAAAAACAAACCTGTGATTT+TGG + chr1.4:65288328-65288347 None:intergenic 25.0%
!! AGATTTTCCTTATTATCTCA+TGG - chr1.4:65289028-65289047 MS.gene036427:intron 25.0%
!! GGATAAGAAGTACAAAATAA+AGG + chr1.4:65288998-65289017 None:intergenic 25.0%
!! GTGTTTTCTAAATTTTCAAC+TGG + chr1.4:65290381-65290400 None:intergenic 25.0%
!! GTTAAAATTTTGTCATTCAC+CGG - chr1.4:65289451-65289470 MS.gene036427:intron 25.0%
!!! TGCATTTAGGTTCTTTTTTT+AGG - chr1.4:65288587-65288606 MS.gene036427:intron 25.0%
AAAAAAAATGTACCTGCAGA+TGG + chr1.4:65289134-65289153 None:intergenic 30.0%
AAACGGAAATACATAACAAC+TGG + chr1.4:65289524-65289543 None:intergenic 30.0%
AAACGGTTAAGATACATACA+GGG + chr1.4:65288682-65288701 None:intergenic 30.0%
AAATAACAGTAGGTCTCAAA+CGG + chr1.4:65288699-65288718 None:intergenic 30.0%
AAGGAAAATCTGTAATGAGA+AGG + chr1.4:65289019-65289038 None:intergenic 30.0%
AAGGTATGTGCAAGTTTATA+CGG - chr1.4:65289268-65289287 MS.gene036427:CDS 30.0%
AATGAAGGTCACGAAAAAAT+TGG - chr1.4:65290402-65290421 MS.gene036427:CDS 30.0%
AATTATAGATAAGTAGGGGA+TGG + chr1.4:65289768-65289787 None:intergenic 30.0%
AGATCATTAACTTCTTTGGA+TGG + chr1.4:65288851-65288870 None:intergenic 30.0%
AGGAAGATCATTAACTTCTT+TGG + chr1.4:65288855-65288874 None:intergenic 30.0%
AGTTATAATAACAGAGCACA+AGG + chr1.4:65290217-65290236 None:intergenic 30.0%
ATACTATACTTACATGCAGA+TGG + chr1.4:65288641-65288660 None:intergenic 30.0%
ATGATTCTATTCAGCCAAAT+TGG - chr1.4:65288269-65288288 MS.gene036427:intron 30.0%
ATTATAGATAAGTAGGGGAT+GGG + chr1.4:65289767-65289786 None:intergenic 30.0%
CACGAAAAAATTGGAATCAA+TGG - chr1.4:65290411-65290430 MS.gene036427:CDS 30.0%
CATCTTCCTTACAAAATATG+GGG + chr1.4:65290193-65290212 None:intergenic 30.0%
GAAGCATGAACATTGAAAAA+CGG + chr1.4:65289541-65289560 None:intergenic 30.0%
GTCATCTTCCTTACAAAATA+TGG + chr1.4:65290195-65290214 None:intergenic 30.0%
GTTAAGCGAAAAATAACAGT+AGG + chr1.4:65288709-65288728 None:intergenic 30.0%
TAATGTAACTAGCTAGTTGT+GGG - chr1.4:65288536-65288555 MS.gene036427:intron 30.0%
TAGTAAACACTAGATCTGTT+CGG + chr1.4:65289953-65289972 None:intergenic 30.0%
TATAACCTATGAAAAAGACG+TGG - chr1.4:65289914-65289933 MS.gene036427:intron 30.0%
TATTAAACCCTAAAGAGAAC+AGG + chr1.4:65289593-65289612 None:intergenic 30.0%
TATTTCTCCTGTTCTCTTTA+GGG - chr1.4:65289583-65289602 MS.gene036427:CDS 30.0%
TCACAAACAAAACATCTTAC+TGG + chr1.4:65289387-65289406 None:intergenic 30.0%
TTACTTAGGAGCTTCTTTAT+GGG - chr1.4:65289158-65289177 MS.gene036427:intron 30.0%
TTATGATGCACATGAAAATC+AGG - chr1.4:65288488-65288507 MS.gene036427:intron 30.0%
TTGAACTTGATGAAAGTTGA+GGG - chr1.4:65289084-65289103 MS.gene036427:intron 30.0%
TTGGATGAATCAAAGAATAG+TGG - chr1.4:65289647-65289666 MS.gene036427:CDS 30.0%
TTTACTTAGGAGCTTCTTTA+TGG - chr1.4:65289157-65289176 MS.gene036427:intron 30.0%
! TTAACTCCCCATATTTTGTA+AGG - chr1.4:65290184-65290203 MS.gene036427:intron 30.0%
! TTATTTCTCCTGTTCTCTTT+AGG - chr1.4:65289582-65289601 MS.gene036427:CDS 30.0%
! TTTTCACATGATAGATGGTA+AGG + chr1.4:65289110-65289129 None:intergenic 30.0%
!!! CACGTTTTTAAGTCTATAGA+CGG + chr1.4:65289741-65289760 None:intergenic 30.0%
AACTCCTTTAACATTGGGAT+AGG - chr1.4:65289494-65289513 MS.gene036427:intron 35.0%
AACTGTACATATTGGAAACG+AGG + chr1.4:65289994-65290013 None:intergenic 35.0%
AATACAAGCACAGAGCAAAA+GGG - chr1.4:65290471-65290490 MS.gene036427:CDS 35.0%
AATGACCATGTGGAATACAA+TGG - chr1.4:65288172-65288191 MS.gene036427:CDS 35.0%
ACATCTTACTGGAGACATTA+CGG + chr1.4:65289376-65289395 None:intergenic 35.0%
AGATTCAGATTGAGAACCAA+GGG - chr1.4:65290274-65290293 MS.gene036427:CDS 35.0%
ATATTGGAAACGAGGACAAT+AGG + chr1.4:65289986-65290005 None:intergenic 35.0%
ATGCATCTTGTTCCTCTATA+TGG - chr1.4:65288430-65288449 MS.gene036427:CDS 35.0%
ATTAATGTCTAGAGACGCTT+TGG - chr1.4:65290014-65290033 MS.gene036427:intron 35.0%
ATTGAAGTCAACTTCTTCTC+TGG + chr1.4:65289708-65289727 None:intergenic 35.0%
CAAACGGTTAAGATACATAC+AGG + chr1.4:65288683-65288702 None:intergenic 35.0%
CAATTGAGAGATGTCATTGT+TGG + chr1.4:65289823-65289842 None:intergenic 35.0%
CATGTGCATCATAAACTAGT+CGG + chr1.4:65288482-65288501 None:intergenic 35.0%
CCAAATTGGAATGAACTTCT+CGG - chr1.4:65288283-65288302 MS.gene036427:intron 35.0%
CCTTCAACTCCTTTAACATT+GGG - chr1.4:65289489-65289508 MS.gene036427:intron 35.0%
CTTGAACTTGATGAAAGTTG+AGG - chr1.4:65289083-65289102 MS.gene036427:intron 35.0%
GTAATGTAACTAGCTAGTTG+TGG - chr1.4:65288535-65288554 MS.gene036427:intron 35.0%
TACAAACCAGAATCATCAGA+AGG - chr1.4:65289249-65289268 MS.gene036427:intron 35.0%
TATGACAGAACCAAAATCAC+AGG - chr1.4:65288315-65288334 MS.gene036427:intron 35.0%
TCCTTCAACTCCTTTAACAT+TGG - chr1.4:65289488-65289507 MS.gene036427:intron 35.0%
TGTTAAAGGAGTTGAAGGAT+CGG + chr1.4:65289487-65289506 None:intergenic 35.0%
TTCAGAAATATAGAACTGCC+AGG - chr1.4:65289226-65289245 MS.gene036427:intron 35.0%
TTTGCCTATCCCAATGTTAA+AGG + chr1.4:65289501-65289520 None:intergenic 35.0%
! AATTCCAACATCAGAAGTGT+TGG - chr1.4:65287989-65288008 MS.gene036427:CDS 35.0%
! ACATAACGTCACTTTCTTGA+GGG + chr1.4:65288093-65288112 None:intergenic 35.0%
! ATGGCTTTTCACATGATAGA+TGG + chr1.4:65289115-65289134 None:intergenic 35.0%
! CTCATGGTGTTAATATGTAG+AGG - chr1.4:65289044-65289063 MS.gene036427:intron 35.0%
!! AGAATGTATTTCTGAAGCAG+AGG + chr1.4:65288062-65288081 None:intergenic 35.0%
!! ATACAATGGTTCTGAAGTCT+TGG - chr1.4:65288186-65288205 MS.gene036427:CDS 35.0%
!! GGATATGCACCAATTTGTTT+TGG - chr1.4:65289628-65289647 MS.gene036427:CDS 35.0%
!! TACAATGGTTCTGAAGTCTT+GGG - chr1.4:65288187-65288206 MS.gene036427:CDS 35.0%
!! TGATTTGCTTTGGTGCATTT+AGG - chr1.4:65288574-65288593 MS.gene036427:intron 35.0%
!! TTTCTGAAGCAGAGGAAAAA+TGG + chr1.4:65288054-65288073 None:intergenic 35.0%
!!! TATGGGTGTGTTTTAATCGA+AGG - chr1.4:65289175-65289194 MS.gene036427:intron 35.0%
AAGAAAGTGACGTTATGTCC+TGG - chr1.4:65288095-65288114 MS.gene036427:CDS 40.0%
AATCTAAGCCTAGAATGCGT+TGG - chr1.4:65288900-65288919 MS.gene036427:intron 40.0%
AATGCTTCTGTAATCCCCTT+AGG + chr1.4:65290056-65290075 None:intergenic 40.0%
AGAAAGTGACGTTATGTCCT+GGG - chr1.4:65288096-65288115 MS.gene036427:CDS 40.0%
AGATCACACAGAAAACACAG+TGG + chr1.4:65290145-65290164 None:intergenic 40.0%
ATATGTAGAGGCTACTCCTA+AGG - chr1.4:65289056-65289075 MS.gene036427:intron 40.0%
ATCAAGTTCAAGACACCCTT+AGG + chr1.4:65289075-65289094 None:intergenic 40.0%
ATGCACCAAAGCAAATCATG+CGG + chr1.4:65288572-65288591 None:intergenic 40.0%
ATTGTGGTTGTATAGGCCTA+AGG - chr1.4:65290037-65290056 MS.gene036427:intron 40.0%
CACATACCTTCTGATGATTC+TGG + chr1.4:65289258-65289277 None:intergenic 40.0%
CACATGAAAATCAGGTCAAG+TGG - chr1.4:65288496-65288515 MS.gene036427:intron 40.0%
CAGAACCATTGTATTCCACA+TGG + chr1.4:65288180-65288199 None:intergenic 40.0%
CAGATTCAGATTGAGAACCA+AGG - chr1.4:65290273-65290292 MS.gene036427:CDS 40.0%
CATCTTACTGGAGACATTAC+GGG + chr1.4:65289375-65289394 None:intergenic 40.0%
CCCAATGTTAAAGGAGTTGA+AGG + chr1.4:65289492-65289511 None:intergenic 40.0%
CCGAGAAGTTCATTCCAATT+TGG + chr1.4:65288286-65288305 None:intergenic 40.0%
CTTGTTCCTCTATATGGTTG+AGG - chr1.4:65288436-65288455 MS.gene036427:CDS 40.0%
GAAAATCAGGTCAAGTGGTA+TGG - chr1.4:65288501-65288520 MS.gene036427:intron 40.0%
GAATACAAGCACAGAGCAAA+AGG - chr1.4:65290470-65290489 MS.gene036427:CDS 40.0%
GAGGAAAAATGGACGTTATC+AGG + chr1.4:65288043-65288062 None:intergenic 40.0%
GTAGTCGACACAGAAGAATT+AGG + chr1.4:65288875-65288894 None:intergenic 40.0%
GTATGTGCAAGTTTATACGG+AGG - chr1.4:65289271-65289290 MS.gene036427:CDS 40.0%
GTATTCCACATGGTCATTCT+GGG + chr1.4:65288170-65288189 None:intergenic 40.0%
GTTAAAGGAGTTGAAGGATC+GGG + chr1.4:65289486-65289505 None:intergenic 40.0%
GTTTGAGCAACAGATGAAGT+CGG - chr1.4:65290314-65290333 MS.gene036427:CDS 40.0%
TAAGATACATACAGGGCAAG+AGG + chr1.4:65288675-65288694 None:intergenic 40.0%
TATGTAGAGGCTACTCCTAA+GGG - chr1.4:65289057-65289076 MS.gene036427:intron 40.0%
TCTCTTGCATCAAGAGAGAA+GGG + chr1.4:65288793-65288812 None:intergenic 40.0%
TCTGTGTGATCTTTGTGTGA+AGG - chr1.4:65290153-65290172 MS.gene036427:intron 40.0%
TGTATTCCACATGGTCATTC+TGG + chr1.4:65288171-65288190 None:intergenic 40.0%
TTAAAGGAGTTGAAGGATCG+GGG + chr1.4:65289485-65289504 None:intergenic 40.0%
TTGCTCTGTGCTTGTATTCT+GGG + chr1.4:65290469-65290488 None:intergenic 40.0%
TTGTGGTTGTATAGGCCTAA+GGG - chr1.4:65290038-65290057 MS.gene036427:intron 40.0%
TTTGCTCTGTGCTTGTATTC+TGG + chr1.4:65290470-65290489 None:intergenic 40.0%
! AAACAACGGATTTCAAGGAG+TGG - chr1.4:65288221-65288240 MS.gene036427:CDS 40.0%
! AACAACGGATTTCAAGGAGT+GGG - chr1.4:65288222-65288241 MS.gene036427:CDS 40.0%
! ACTTCTTTGGATGGTAGTGA+TGG + chr1.4:65288842-65288861 None:intergenic 40.0%
! ATCTGTTCGGCTCAGTTTTT+AGG + chr1.4:65289940-65289959 None:intergenic 40.0%
! CTCCTTGAAATCCGTTGTTT+TGG + chr1.4:65288221-65288240 None:intergenic 40.0%
! GACATAACGTCACTTTCTTG+AGG + chr1.4:65288094-65288113 None:intergenic 40.0%
! GCTTTGGATTGTGGTTGTAT+AGG - chr1.4:65290030-65290049 MS.gene036427:intron 40.0%
! TCAGTCTTCACTCGTTTTGT+GGG + chr1.4:65290555-65290574 None:intergenic 40.0%
! TGCCAAAACAACGGATTTCA+AGG - chr1.4:65288216-65288235 MS.gene036427:CDS 40.0%
!! TGATGATTCTGGTTTGTACC+TGG + chr1.4:65289247-65289266 None:intergenic 40.0%
!! TTAGGCCACGTCTTTTTCAT+AGG + chr1.4:65289922-65289941 None:intergenic 40.0%
!! TTCTTCTCTGGAATTCCTGT+AGG + chr1.4:65289696-65289715 None:intergenic 40.0%
!!! ACATTTTTTTCCCTGCCTAC+AGG - chr1.4:65289678-65289697 MS.gene036427:CDS 40.0%
ACAAAGGCCTCATGAAGTTC+TGG + chr1.4:65288929-65288948 None:intergenic 45.0%
ACGTTATCAGGCTGTTCAGA+AGG + chr1.4:65288031-65288050 None:intergenic 45.0%
ACTTCAGACAACATGCCTGA+AGG - chr1.4:65290438-65290457 MS.gene036427:CDS 45.0%
AGAACTTCATGAGGCCTTTG+TGG - chr1.4:65288928-65288947 MS.gene036427:intron 45.0%
ATACAGGGCAAGAGGAAAGA+GGG + chr1.4:65288667-65288686 None:intergenic 45.0%
ATACTGCTGCTGTGAAACCT+GGG + chr1.4:65288816-65288835 None:intergenic 45.0%
CACGATCCTCAACCATATAG+AGG + chr1.4:65288445-65288464 None:intergenic 45.0%
CGTTATCAGGCTGTTCAGAA+GGG + chr1.4:65288030-65288049 None:intergenic 45.0%
CTCTATATGGTTGAGGATCG+TGG - chr1.4:65288443-65288462 MS.gene036427:CDS 45.0%
CTCTTGCATCAAGAGAGAAG+GGG + chr1.4:65288792-65288811 None:intergenic 45.0%
CTGATTGACTGCTTCCACAA+AGG + chr1.4:65288945-65288964 None:intergenic 45.0%
GAAAGTGACGTTATGTCCTG+GGG - chr1.4:65288097-65288116 MS.gene036427:CDS 45.0%
GATAGTGCCACACTTGATAG+TGG + chr1.4:65290354-65290373 None:intergenic 45.0%
GGAGATGATGCTAAAGCTAC+CGG - chr1.4:65290492-65290511 MS.gene036427:CDS 45.0%
GGGTGGAAATGCCAAAACAA+CGG - chr1.4:65288207-65288226 MS.gene036427:CDS 45.0%
GTCTCTTGCATCAAGAGAGA+AGG + chr1.4:65288794-65288813 None:intergenic 45.0%
GTGTCCAACACTTCTGATGT+TGG + chr1.4:65287996-65288015 None:intergenic 45.0%
GTTATCAGGCTGTTCAGAAG+GGG + chr1.4:65288029-65288048 None:intergenic 45.0%
TATACTGCTGCTGTGAAACC+TGG + chr1.4:65288817-65288836 None:intergenic 45.0%
TCATCTGTAGATGCTTCCCT+TGG + chr1.4:65290293-65290312 None:intergenic 45.0%
TCATGTGAAAAGCCATCTGC+AGG - chr1.4:65289119-65289138 MS.gene036427:intron 45.0%
TGTGGTTGTATAGGCCTAAG+GGG - chr1.4:65290039-65290058 MS.gene036427:intron 45.0%
TTCAAATGGATCAGTTCCCC+AGG + chr1.4:65288116-65288135 None:intergenic 45.0%
TTCTGCTCCACTATCAAGTG+TGG - chr1.4:65290344-65290363 MS.gene036427:CDS 45.0%
TTCTGGGTGGGAACATTATC+AGG + chr1.4:65288154-65288173 None:intergenic 45.0%
TTGGACTCCAGAACTTCATG+AGG - chr1.4:65288919-65288938 MS.gene036427:intron 45.0%
! GTATTCTGGGAACTTCCTTC+AGG + chr1.4:65290456-65290475 None:intergenic 45.0%
! GTCAGTCTTCACTCGTTTTG+TGG + chr1.4:65290556-65290575 None:intergenic 45.0%
! TCTAGAGACGCTTTGGATTG+TGG - chr1.4:65290021-65290040 MS.gene036427:intron 45.0%
!! AATGGTTCTGAAGTCTTGGG+TGG - chr1.4:65288190-65288209 MS.gene036427:CDS 45.0%
!! ACACTTGATAGTGGAGCAGA+AGG + chr1.4:65290345-65290364 None:intergenic 45.0%
!! AGGGGATTACAGAAGCATTG+CGG - chr1.4:65290057-65290076 MS.gene036427:intron 45.0%
!!! AATTAATTATTTTAATGAAT+AGG - chr1.4:65288754-65288773 MS.gene036427:intron 5.0%
!!! TATTTAATTTTTCTTTTTAT+AGG - chr1.4:65289407-65289426 MS.gene036427:CDS 5.0%
AAGGATCGGGGTTCAAACTC+CGG + chr1.4:65289473-65289492 None:intergenic 50.0%
AATTGATGCAGCCTGCATCG+AGG + chr1.4:65287969-65287988 None:intergenic 50.0%
CATACAGGGCAAGAGGAAAG+AGG + chr1.4:65288668-65288687 None:intergenic 50.0%
GAAGCAGTCAATCAGCTTGG+TGG - chr1.4:65288950-65288969 MS.gene036427:intron 50.0%
GCGGCTTCATGAACAACTTG+AGG - chr1.4:65290100-65290119 MS.gene036427:intron 50.0%
GTGGAAGCAGTCAATCAGCT+TGG - chr1.4:65288947-65288966 MS.gene036427:intron 50.0%
GTTGTTCATGAAGCCGCTTC+TGG + chr1.4:65290097-65290116 None:intergenic 50.0%
TATTCCCTCTTCCTCGATGC+AGG - chr1.4:65287955-65287974 MS.gene036427:CDS 50.0%
TCCACATGGTCATTCTGGGT+GGG + chr1.4:65288166-65288185 None:intergenic 50.0%
TCTCTTGATGCAAGAGACCC+AGG - chr1.4:65288796-65288815 MS.gene036427:CDS 50.0%
TGCAGATGGAACTCCAGAAG+CGG - chr1.4:65290081-65290100 MS.gene036427:intron 50.0%
TTCCACATGGTCATTCTGGG+TGG + chr1.4:65288167-65288186 None:intergenic 50.0%
TTCTCCCAGCTCAAGTTCAC+CGG + chr1.4:65290514-65290533 None:intergenic 50.0%
! GTCTTCACTCGTTTTGTGGG+TGG + chr1.4:65290552-65290571 None:intergenic 50.0%
! TCTGGAGTCCAACGCATTCT+AGG + chr1.4:65288911-65288930 None:intergenic 50.0%
!! CTCTGGAATTCCTGTAGGCA+GGG + chr1.4:65289691-65289710 None:intergenic 50.0%
!! TCTCTGGAATTCCTGTAGGC+AGG + chr1.4:65289692-65289711 None:intergenic 50.0%
AGCTACCGGTGAACTTGAGC+TGG - chr1.4:65290506-65290525 MS.gene036427:CDS 55.0%
GCTACCGGTGAACTTGAGCT+GGG - chr1.4:65290507-65290526 MS.gene036427:CDS 55.0%
TCCCACCCAGAATGACCATG+TGG - chr1.4:65288162-65288181 MS.gene036427:CDS 55.0%
TTGTGGGTGGTGCGGTAAGT+TGG + chr1.4:65290539-65290558 None:intergenic 55.0%
! CACTCGTTTTGTGGGTGGTG+CGG + chr1.4:65290547-65290566 None:intergenic 55.0%
!! AGAAGCATTGCGGCTGCAGA+TGG - chr1.4:65290067-65290086 MS.gene036427:intron 55.0%
!! GAGCGCCGCATGATTTGCTT+TGG - chr1.4:65288564-65288583 MS.gene036427:intron 55.0%
GCAGCCTGCATCGAGGAAGA+GGG + chr1.4:65287962-65287981 None:intergenic 60.0%
TGCAGCCTGCATCGAGGAAG+AGG + chr1.4:65287963-65287982 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 65287944 65290581 65287944 ID=MS.gene036427
chr1.4 mRNA 65287944 65290581 65287944 ID=MS.gene036427.t1;Parent=MS.gene036427
chr1.4 exon 65290189 65290581 65290189 ID=MS.gene036427.t1.exon1;Parent=MS.gene036427.t1
chr1.4 CDS 65290189 65290581 65290189 ID=cds.MS.gene036427.t1;Parent=MS.gene036427.t1
chr1.4 exon 65289545 65289749 65289545 ID=MS.gene036427.t1.exon2;Parent=MS.gene036427.t1
chr1.4 CDS 65289545 65289749 65289545 ID=cds.MS.gene036427.t1;Parent=MS.gene036427.t1
chr1.4 exon 65289385 65289461 65289385 ID=MS.gene036427.t1.exon3;Parent=MS.gene036427.t1
chr1.4 CDS 65289385 65289461 65289385 ID=cds.MS.gene036427.t1;Parent=MS.gene036427.t1
chr1.4 exon 65289255 65289294 65289255 ID=MS.gene036427.t1.exon4;Parent=MS.gene036427.t1
chr1.4 CDS 65289255 65289294 65289255 ID=cds.MS.gene036427.t1;Parent=MS.gene036427.t1
chr1.4 exon 65288768 65288825 65288768 ID=MS.gene036427.t1.exon5;Parent=MS.gene036427.t1
chr1.4 CDS 65288768 65288825 65288768 ID=cds.MS.gene036427.t1;Parent=MS.gene036427.t1
chr1.4 exon 65288404 65288473 65288404 ID=MS.gene036427.t1.exon6;Parent=MS.gene036427.t1
chr1.4 CDS 65288404 65288473 65288404 ID=cds.MS.gene036427.t1;Parent=MS.gene036427.t1
chr1.4 exon 65287944 65288267 65287944 ID=MS.gene036427.t1.exon7;Parent=MS.gene036427.t1
chr1.4 CDS 65287944 65288267 65287944 ID=cds.MS.gene036427.t1;Parent=MS.gene036427.t1
Gene Sequence

>MS.gene036427

ATGTCTTCGTCTATTCCCTCTTCCTCGATGCAGGCTGCATCAATTAATTCCAACATCAGAAGTGTTGGACACATGTTTTCAACCCCTTCTGAACAGCCTGATAACGTCCATTTTTCCTCTGCTTCAGAAATACATTCTATGACATTCCCTCAAGAAAGTGACGTTATGTCCTGGGGAACTGATCCATTTGAAGATATTCTTCAATTTCCTGATAATGTTCCCACCCAGAATGACCATGTGGAATACAATGGTTCTGAAGTCTTGGGTGGAAATGCCAAAACAACGGATTTCAAGGAGTGGGTTGATCAGTTGATGTCAGTCGATGATGATTCTATTCAGCCAAATTGGAATGAACTTCTCGGTGATAACAATATGACAGAACCAAAATCACAGGTTTGTTTTTTTAAACTGATTTTAATATTGTACTACAAGGTCTCAAAGTGCTTGCTCCTATCTGTGAATAATACTAGAATATGTGGATGGCATATGCATCTTGTTCCTCTATATGGTTGAGGATCGTGGTTATTTATTATGACCGACTAGTTTATGATGCACATGAAAATCAGGTCAAGTGGTATGGTATGATTATCTGTAATGTAACTAGCTAGTTGTGGGAAACTGAGCGCCGCATGATTTGCTTTGGTGCATTTAGGTTCTTTTTTTAGGAAAAAAAAAATTGATTCATCAATCATTGCCATCTGCATGTAAGTATAGTATGCTCCCTCTTTCCTCTTGCCCTGTATGTATCTTAACCGTTTGAGACCTACTGTTATTTTTCGCTTAACTCTTTGTCTATATAACTTCTTTTTTAATTAATTATTTTAATGAATAGGATGCACAAATGTCCCCTTCTCTCTTGATGCAAGAGACCCAGGTTTCACAGCAGCAGTATATACCATCACTACCATCCAAAGAAGTTAATGATCTTCCTAATTCTTCTGTGTCGACTACATCGCAATCTAAGCCTAGAATGCGTTGGACTCCAGAACTTCATGAGGCCTTTGTGGAAGCAGTCAATCAGCTTGGTGGTAGTGAGAGTATGTTCACTTCTCCTTTATTTTGTACTTCTTATCCTTCTCATTACAGATTTTCCTTATTATCTCATGGTGTTAATATGTAGAGGCTACTCCTAAGGGTGTCTTGAACTTGATGAAAGTTGAGGGCCTTACCATCTATCATGTGAAAAGCCATCTGCAGGTACATTTTTTTTTTCTTTACTTAGGAGCTTCTTTATGGGTGTGTTTTAATCGAAGGTTTTTAATGAATTATTTGTTCCCTTAATTTCAGAAATATAGAACTGCCAGGTACAAACCAGAATCATCAGAAGGTATGTGCAAGTTTATACGGAGGTTTAATGTTCATATAAAGTTCATATAAACCAGGTTGCTTATTATTTATATCTGTGGTTTAGTGCTTCTTCACTCATCACCCGTAATGTCTCCAGTAAGATGTTTTGTTTGTGATATTTAATTTTTCTTTTTATAGGCAAATACGGTAAATTAGTTATGTTAAAATTTTGTCATTCACCGGAGTTTGAACCCCGATCCTTCAACTCCTTTAACATTGGGATAGGCAAACCAGTTGTTATGTATTTCCGTTTTTCAATGTTCATGCTTCTGTGACTGTATCTGTTCAAATTTATTTCTCCTGTTCTCTTTAGGGTTTAATAAGCACATACATAATTGGATATGCACCAATTTGTTTTGGATGAATCAAAGAATAGTGGTCTGAATGACATTTTTTTCCCTGCCTACAGGAATTCCAGAGAAGAAGTTGACTTCAATCGACGAAATGCCGTCTATAGACTTAAAAACGTGAGTCCCATCCCCTACTTATCTATAATTGTTTTTCTTATTGTATTGATTGATCTTTTTGACCAACAATGACATCTCTCAATTGTATGCATAGAGCTAAGTGTAAATGTCTAAAGTTCATTTGAAATAACTTTTGTCGTATATTTGAATTTAAGATATAACCTATGAAAAAGACGTGGCCTAAAAACTGAGCCGAACAGATCTAGTGTTTACTATTATATGATACCTATTGTCCTCGTTTCCAATATGTACAGTTATTAATGTCTAGAGACGCTTTGGATTGTGGTTGTATAGGCCTAAGGGGATTACAGAAGCATTGCGGCTGCAGATGGAACTCCAGAAGCGGCTTCATGAACAACTTGAGGTATATCTTAGTTGCTAAATCCACTGTGTTTTCTGTGTGATCTTTGTGTGAAGGAGTGTTGTTTAACTCCCCATATTTTGTAAGGAAGATGACCTTGTGCTCTGTTATTATAACTATAATAACAACTATTATGCAGATTCAAAGAAATTTACAGATTCAGATTGAGAACCAAGGGAAGCATCTACAGATGATGTTTGAGCAACAGATGAAGTCGGATGAGCCTTCTGCTCCACTATCAAGTGTGGCACTATCTTCTCCAGTTGAAAATTTAGAAAACACAAATGAAGGTCACGAAAAAATTGGAATCAATGGCAGCACTTCAGACAACATGCCTGAAGGAAGTTCCCAGAATACAAGCACAGAGCAAAAGGGAGATGATGCTAAAGCTACCGGTGAACTTGAGCTGGGAGAAGACCAACTTACCGCACCACCCACAAAACGAGTGAAGACTGACAAGTAG
Protein sequence

>MS.gene036427.t1

MSSSIPSSSMQAASINSNIRSVGHMFSTPSEQPDNVHFSSASEIHSMTFPQESDVMSWGTDPFEDILQFPDNVPTQNDHVEYNGSEVLGGNAKTTDFKEWVDQLMSVDDDSIQPNWNELLGDNNMTEPKSQDAQMSPSLLMQETQVSQQQYIPSLPSKEVNDLPNSSVSTTSQSKPRMRWTPELHEAFVEAVNQLGGSEKATPKGVLNLMKVEGLTIYHVKSHLQKYRTARYKPESSEGIPEKKLTSIDEMPSIDLKTPKGITEALRLQMELQKRLHEQLEIQRNLQIQIENQGKHLQMMFEQQMKSDEPSAPLSSVALSSPVENLENTNEGHEKIGINGSTSDNMPEGSSQNTSTEQKGDDAKATGELELGEDQLTAPPTKRVKTDK