AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene036495

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036495.t1 MTR_4g120380 77.540 374 76 4 1 368 1 372 0.0 577
MS.gene036495.t1 MTR_4g120380 79.261 352 65 4 1 346 1 350 0.0 552
MS.gene036495.t1 MTR_4g119580 72.776 371 97 3 1 367 1 371 0.0 510
MS.gene036495.t1 MTR_2g436460 50.909 330 161 1 37 365 44 373 1.59e-112 333
MS.gene036495.t1 MTR_4g119570 64.940 251 55 1 117 367 13 230 9.41e-110 320
MS.gene036495.t1 MTR_2g437260 46.647 343 181 1 26 366 22 364 3.41e-109 325
MS.gene036495.t1 MTR_4g119550 50.298 336 162 2 32 366 47 378 9.78e-109 324
MS.gene036495.t1 MTR_2g437040 48.824 340 169 4 30 366 31 368 7.50e-108 321
MS.gene036495.t1 MTR_2g437030 48.352 364 184 2 7 366 91 454 1.85e-105 318
MS.gene036495.t1 MTR_2g436440 44.652 374 199 2 1 366 1 374 2.93e-105 315
MS.gene036495.t1 MTR_2g437120 49.267 341 159 4 35 366 46 381 1.22e-103 311
MS.gene036495.t1 MTR_2g076320 48.235 340 163 3 35 365 45 380 1.98e-102 308
MS.gene036495.t1 MTR_2g437960 48.204 334 160 3 42 366 55 384 2.92e-102 308
MS.gene036495.t1 MTR_2g436400 46.041 341 181 2 28 366 36 375 3.18e-102 307
MS.gene036495.t1 MTR_2g437990 48.512 336 169 2 35 366 45 380 3.87e-102 308
MS.gene036495.t1 MTR_2g437960 48.204 334 160 3 42 366 55 384 5.09e-102 308
MS.gene036495.t1 MTR_2g438010 48.071 337 170 2 35 366 65 401 5.92e-102 308
MS.gene036495.t1 MTR_2g437060 44.324 370 192 5 4 366 21 383 6.19e-102 306
MS.gene036495.t1 MTR_2g437080 46.988 332 174 1 37 366 37 368 4.68e-101 304
MS.gene036495.t1 MTR_2g437130 45.109 368 194 3 1 366 8 369 6.37e-101 304
MS.gene036495.t1 MTR_2g437160 47.230 343 177 2 28 366 51 393 5.49e-100 302
MS.gene036495.t1 MTR_2g437200 44.180 378 173 2 27 366 19 396 5.67e-100 301
MS.gene036495.t1 MTR_2g437020 46.175 366 187 5 5 366 9 368 3.50e-99 299
MS.gene036495.t1 MTR_2g437100 49.096 332 162 3 42 366 62 393 2.47e-98 298
MS.gene036495.t1 MTR_2g437150 48.083 339 169 3 35 366 50 388 6.51e-98 296
MS.gene036495.t1 MTR_2g437170 47.788 339 170 3 35 366 50 388 2.57e-97 295
MS.gene036495.t1 MTR_2g437240 43.669 387 165 5 27 369 22 399 5.65e-94 287
MS.gene036495.t1 MTR_2g437180 46.505 329 169 3 42 365 51 377 1.61e-92 283
MS.gene036495.t1 MTR_2g438020 43.251 363 168 2 42 366 73 435 1.70e-89 278
MS.gene036495.t1 MTR_2g437940 44.954 327 153 3 42 366 51 352 1.31e-86 266
MS.gene036495.t1 MTR_2g019810 42.857 336 178 4 38 366 29 357 2.03e-85 263
MS.gene036495.t1 MTR_2g019840 41.600 375 204 6 1 366 1 369 2.86e-85 263
MS.gene036495.t1 MTR_3g450500 32.432 370 244 2 4 367 2 371 1.78e-61 202
MS.gene036495.t1 MTR_4g007490 33.708 356 222 5 22 366 16 368 1.87e-61 202
MS.gene036495.t1 MTR_2g437090 55.195 154 69 0 213 366 3 156 2.24e-52 172
MS.gene036495.t1 MTR_2g437160 50.296 169 84 0 198 366 134 302 4.67e-52 176
MS.gene036495.t1 MTR_2g437160 45.055 91 48 1 28 116 51 141 3.88e-15 75.9
MS.gene036495.t1 MTR_8g080990 27.893 337 239 3 35 367 37 373 3.74e-36 135
MS.gene036495.t1 MTR_2g436370 41.111 180 94 5 4 176 15 189 9.57e-35 127
MS.gene036495.t1 MTR_8g081000 25.223 337 248 3 35 367 50 386 3.53e-31 122
MS.gene036495.t1 MTR_8g081000 25.223 337 248 3 35 367 50 386 4.53e-31 122
MS.gene036495.t1 MTR_8g081000 25.223 337 248 3 35 367 50 386 5.47e-31 122
MS.gene036495.t1 MTR_4g119615 88.235 51 6 0 310 360 26 76 1.40e-23 94.0
MS.gene036495.t1 MTR_2g436380 40.000 105 45 3 213 316 3 90 1.88e-14 73.6
MS.gene036495.t1 MTR_4g049400 37.600 125 69 3 22 137 25 149 2.49e-13 69.3
MS.gene036495.t1 MTR_3g085240 30.508 177 108 3 40 215 57 219 1.22e-11 64.7
MS.gene036495.t1 MTR_8g012210 23.232 297 203 10 84 366 63 348 1.60e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene036495.t1 AT5G07900 34.337 332 212 3 42 367 70 401 3.33e-64 210
MS.gene036495.t1 AT1G21150 30.909 330 222 3 40 363 55 384 6.24e-47 164
MS.gene036495.t1 AT1G21150 30.909 330 222 3 40 363 119 448 1.22e-46 165
MS.gene036495.t1 AT1G21150 30.982 326 219 3 44 363 23 348 1.88e-46 162
MS.gene036495.t1 AT1G61980 29.348 368 219 7 40 367 51 417 2.94e-44 158
MS.gene036495.t1 AT1G61980 29.348 368 219 7 40 367 51 417 2.94e-44 158
MS.gene036495.t1 AT1G61980 29.348 368 219 7 40 367 51 417 2.94e-44 158
MS.gene036495.t1 AT1G62120 27.520 367 225 6 42 367 57 423 1.93e-42 153
MS.gene036495.t1 AT1G61970 26.975 367 225 8 42 367 53 417 2.51e-40 147
MS.gene036495.t1 AT1G61970 26.975 367 225 8 42 367 53 417 2.51e-40 147
MS.gene036495.t1 AT1G61970 26.975 367 225 8 42 367 53 417 2.51e-40 147
MS.gene036495.t1 AT1G61970 26.975 367 225 8 42 367 53 417 2.51e-40 147
MS.gene036495.t1 AT1G61970 26.975 367 225 8 42 367 53 417 2.51e-40 147
MS.gene036495.t1 AT1G61970 26.975 367 225 8 42 367 53 417 2.51e-40 147
MS.gene036495.t1 AT1G61970 26.975 367 225 8 42 367 53 417 2.51e-40 147
MS.gene036495.t1 AT1G61990 28.261 368 218 7 42 370 53 413 7.26e-40 146
MS.gene036495.t1 AT1G61990 28.261 368 218 7 42 370 53 413 7.26e-40 146
MS.gene036495.t1 AT5G64950 28.612 353 239 5 26 366 25 376 3.52e-33 127
MS.gene036495.t1 AT3G46950 31.667 240 159 4 42 280 53 288 1.24e-32 127
MS.gene036495.t1 AT1G62085 29.545 264 178 3 25 280 34 297 1.46e-29 119
MS.gene036495.t1 AT1G62085 30.328 122 82 2 249 367 336 457 3.16e-11 65.1
MS.gene036495.t1 AT1G62085 29.545 264 178 3 25 280 34 297 1.46e-29 119
MS.gene036495.t1 AT1G62085 30.328 122 82 2 249 367 336 457 3.16e-11 65.1
MS.gene036495.t1 AT1G62150 24.156 385 216 8 42 351 59 442 2.75e-29 118
MS.gene036495.t1 AT1G62010 23.944 355 219 8 57 368 56 402 3.91e-28 114
MS.gene036495.t1 AT1G62110 26.692 266 192 2 23 285 34 299 1.70e-27 113
MS.gene036495.t1 AT1G62110 22.967 209 140 5 175 368 256 458 7.89e-13 69.7
MS.gene036495.t1 AT1G61960 28.395 243 168 4 42 280 53 293 1.25e-25 107
MS.gene036495.t1 AT1G61960 28.689 122 84 1 249 367 332 453 7.71e-12 67.0
MS.gene036495.t1 AT1G56380 24.931 361 219 10 44 357 30 385 3.02e-25 105
MS.gene036495.t1 AT1G56380 24.931 361 219 10 44 357 19 374 3.63e-25 105
MS.gene036495.t1 AT5G23930 28.346 254 169 5 25 270 32 280 1.22e-24 105
MS.gene036495.t1 AT1G79220 25.000 296 200 11 84 366 91 377 8.59e-11 63.5

Find 105 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAGGAGATTAAGGAATT+AGG 0.141632 8.4:+12183898 MS.gene036495:CDS
GTGTTCTGAAGATGATATTT+TGG 0.207060 8.4:+12184023 MS.gene036495:CDS
AAAGGCTTTGGAGGAGATTA+AGG 0.223358 8.4:+12183891 MS.gene036495:CDS
ATGCTCTACTGATTTGAAAA+AGG 0.232177 8.4:+12183873 MS.gene036495:CDS
AAAGGTAACATTTCGGGGTT+TGG 0.265770 8.4:-12183294 None:intergenic
GGACTTGGTGACAGCCCTTT+TGG 0.271549 8.4:-12183962 None:intergenic
TTGGGATCCTTTATTGCTCC+TGG 0.276197 8.4:+12184131 MS.gene036495:CDS
AACTCGTCCTTCAAATAAAA+TGG 0.276985 8.4:-12184267 None:intergenic
CAAAAGTATGTGAAACGTTT+TGG 0.285164 8.4:+12184297 MS.gene036495:CDS
AAGGTAACATTTCGGGGTTT+GGG 0.285785 8.4:-12183293 None:intergenic
CATGCTATTAAAGCTATATC+AGG 0.320327 8.4:+12184332 MS.gene036495:CDS
CTTTGGCAAAATTGATTTGA+TGG 0.324194 8.4:-12183569 None:intergenic
TGGAGGAGATTAAGGAATTA+GGG 0.324678 8.4:+12183899 MS.gene036495:CDS
AGTGGGTTCGGTGAAGAAAA+AGG 0.366164 8.4:-12183312 None:intergenic
GTCTTGGGGAGAAGCACCTT+TGG 0.383783 8.4:-12183608 None:intergenic
AACGGTGGCAACAATGTCTT+GGG 0.392461 8.4:-12183623 None:intergenic
GAGGCTTGAAGCAAGGAAAC+GGG 0.394445 8.4:-12183656 None:intergenic
GTATCTTCTTTCCAAAGGTT+TGG 0.398152 8.4:+12184218 MS.gene036495:CDS
GATAACGGAGTCGGGTTTGT+TGG 0.416971 8.4:-12183464 None:intergenic
CTTCTTACCAATTCAAAAGA+AGG 0.421065 8.4:-12183694 None:intergenic
AGTTGATGCCTTGAAGAGTT+GGG 0.424925 8.4:+12183999 MS.gene036495:CDS
TGGGATCCTTTATTGCTCCT+GGG 0.425385 8.4:+12184132 MS.gene036495:CDS
TTGGGTCAAACAATTTGGTT+GGG 0.428878 8.4:+12184113 MS.gene036495:CDS
TGCGAATGATGGTTTGTATC+TGG 0.432299 8.4:-12183519 None:intergenic
TTGTTGGTGAGGTAGGAAAC+TGG 0.434401 8.4:-12183382 None:intergenic
GTGTGGCATTATTGGCCAAA+AGG 0.435093 8.4:+12183947 MS.gene036495:CDS
TTTGGCATCCCATTGGGACT+TGG 0.436785 8.4:-12183977 None:intergenic
ACTCTTCAAGGCATCAACTT+TGG 0.437967 8.4:-12183995 None:intergenic
ATCCTCCTTCACCAAACCTT+TGG 0.439740 8.4:-12184229 None:intergenic
ATAACGGAGTCGGGTTTGTT+GGG 0.446265 8.4:-12183463 None:intergenic
GGAGACCGTAGCATTAAGTT+AGG 0.446307 8.4:-12184063 None:intergenic
AACCTTTGGAAAGAAGATAC+TGG 0.447858 8.4:-12184215 None:intergenic
ATCAACTTTGGCATCCCATT+GGG 0.451567 8.4:-12183983 None:intergenic
TAACTTAATGCTACGGTCTC+CGG 0.453116 8.4:+12184065 MS.gene036495:CDS
ATACTAAATCCGAAAAGAAC+GGG 0.459485 8.4:-12184159 None:intergenic
TTTGGCAAAATTGATTTGAT+GGG 0.461357 8.4:-12183568 None:intergenic
CGAGGCTTGAAGCAAGGAAA+CGG 0.463064 8.4:-12183657 None:intergenic
AAGCGAGAATCACGAATTGT+AGG 0.464569 8.4:-12183766 None:intergenic
GTCCAGTATCTTCTTTCCAA+AGG 0.465341 8.4:+12184213 MS.gene036495:CDS
TGGAGAAGTAGGTGAAAGAG+TGG 0.470779 8.4:-12183330 None:intergenic
GGCTGTCACCAAGTCCCAAT+GGG 0.471957 8.4:+12183969 MS.gene036495:CDS
AGTAGGTGAAAGAGTGGGTT+CGG 0.474844 8.4:-12183324 None:intergenic
CATCAACTTTGGCATCCCAT+TGG 0.475903 8.4:-12183984 None:intergenic
TAACGGTGGCAACAATGTCT+TGG 0.479132 8.4:-12183624 None:intergenic
AGCGAGAATCACGAATTGTA+GGG 0.480852 8.4:-12183765 None:intergenic
AGTGTTGAAACGGAGTCGTT+TGG 0.481325 8.4:-12183440 None:intergenic
TCGGATTTAGTATAGAGAAA+AGG 0.482494 8.4:+12184169 MS.gene036495:CDS
TTGGGGAGAAGCACCTTTGG+AGG 0.490963 8.4:-12183605 None:intergenic
TTTGGGTCAAACAATTTGGT+TGG 0.492308 8.4:+12184112 MS.gene036495:CDS
AAGTTGATGCCTTGAAGAGT+TGG 0.496376 8.4:+12183998 MS.gene036495:CDS
GGTGTGCCCAGGAGCAATAA+AGG 0.500318 8.4:-12184138 None:intergenic
AGGGTCTAGTAGTAAGCAAA+TGG 0.503507 8.4:-12183843 None:intergenic
CGAAAAGAACGGGTGTGCCC+AGG 0.505305 8.4:-12184149 None:intergenic
GTTTGTTGGGAGTGTTGAAA+CGG 0.507083 8.4:-12183450 None:intergenic
AGGTAACATTTCGGGGTTTG+GGG 0.510986 8.4:-12183292 None:intergenic
ATCACCGCATTGAGTTTGTC+CGG 0.516458 8.4:-12184084 None:intergenic
CTGATATAGCTTTAATAGCA+TGG 0.517550 8.4:-12184331 None:intergenic
TGGAGATAAACCGCAGTTGT+TGG 0.517833 8.4:-12183398 None:intergenic
TTCTTACCAATTCAAAAGAA+GGG 0.521054 8.4:-12183693 None:intergenic
TGTGGCATTATTGGCCAAAA+GGG 0.530442 8.4:+12183948 MS.gene036495:CDS
CGATGGAATCCTGCGAATGA+TGG 0.539386 8.4:-12183530 None:intergenic
TTCCAAAGGTTTGGTGAAGG+AGG 0.548687 8.4:+12184227 MS.gene036495:CDS
ATGTGAAAGGGTGGAGAAGT+AGG 0.549547 8.4:-12183341 None:intergenic
GGGCTGTCACCAAGTCCCAA+TGG 0.553920 8.4:+12183968 MS.gene036495:CDS
TATACTAAATCCGAAAAGAA+CGG 0.560431 8.4:-12184160 None:intergenic
GACAACCTAACTTAATGCTA+CGG 0.560893 8.4:+12184058 MS.gene036495:CDS
ATTGTTGATCTGAATGTGAA+AGG 0.561922 8.4:-12183354 None:intergenic
ATGTTTCATTATCACCGCCT+CGG 0.567337 8.4:+12183256 MS.gene036495:CDS
GTAATAGCTTTATGATCAGA+AGG 0.568712 8.4:-12183724 None:intergenic
ACCGCAGTTGTTGGTGAGGT+AGG 0.574996 8.4:-12183389 None:intergenic
GGGTTTGAAACGATGAGCGA+TGG 0.579479 8.4:-12183547 None:intergenic
TCTTTCCAAAGGTTTGGTGA+AGG 0.579695 8.4:+12184224 MS.gene036495:CDS
TTGGCAAAATTGATTTGATG+GGG 0.581662 8.4:-12183567 None:intergenic
AGGCTTGAAGCAAGGAAACG+GGG 0.582746 8.4:-12183655 None:intergenic
AGATACAAACCATCATTCGC+AGG 0.586629 8.4:+12183521 MS.gene036495:CDS
ATCAGTAGAGCATATTGTAG+AGG 0.594418 8.4:-12183863 None:intergenic
TTAAAGCTATATCAGGGAGG+AGG 0.595436 8.4:+12184339 MS.gene036495:CDS
TTGTTGATCTGAATGTGAAA+GGG 0.596672 8.4:-12183353 None:intergenic
AGAGAAAAGGCTTATTCCGA+GGG 0.601279 8.4:+12184182 MS.gene036495:CDS
ATGCTATTAAAGCTATATCA+GGG 0.602620 8.4:+12184333 MS.gene036495:CDS
TGTTGGTGAGGTAGGAAACT+GGG 0.604893 8.4:-12183381 None:intergenic
TTGATCTGAATGTGAAAGGG+TGG 0.604993 8.4:-12183350 None:intergenic
GATGCTTTGAGAGCGTCTTG+TGG 0.606368 8.4:-12183418 None:intergenic
AAGGGATTATGTACTTCTCG+AGG 0.610017 8.4:-12183675 None:intergenic
TCAGTAGAGCATATTGTAGA+GGG 0.610595 8.4:-12183862 None:intergenic
TCTGGTGATCGGAGAAACCG+TGG 0.611970 8.4:-12183501 None:intergenic
TACTGGACAACAGAAGCCCT+CGG 0.620230 8.4:-12184198 None:intergenic
GTTGATGCCTTGAAGAGTTG+GGG 0.620439 8.4:+12184000 MS.gene036495:CDS
GGAGAAGTAGGTGAAAGAGT+GGG 0.620482 8.4:-12183329 None:intergenic
GATGGTTTGTATCTGGTGAT+CGG 0.623257 8.4:-12183512 None:intergenic
TAGAGAAAAGGCTTATTCCG+AGG 0.626765 8.4:+12184181 MS.gene036495:CDS
ACTTCTCGAGGCTTGAAGCA+AGG 0.627237 8.4:-12183663 None:intergenic
CGAGAATCACGAATTGTAGG+GGG 0.639288 8.4:-12183763 None:intergenic
TCCTACCTCACCAACAACTG+CGG 0.644241 8.4:+12183388 MS.gene036495:CDS
ATAAACCGCAGTTGTTGGTG+AGG 0.646663 8.4:-12183393 None:intergenic
GGCTTGAAGCAAGGAAACGG+GGG 0.647192 8.4:-12183654 None:intergenic
GCGAGAATCACGAATTGTAG+GGG 0.656253 8.4:-12183764 None:intergenic
GGTAACATTTCGGGGTTTGG+GGG 0.695526 8.4:-12183291 None:intergenic
TTGATGCCTTGAAGAGTTGG+GGG 0.700177 8.4:+12184001 MS.gene036495:CDS
GGTTGAGAAGAGCTCCGAGG+CGG 0.704891 8.4:-12183270 None:intergenic
CTGGTGATCGGAGAAACCGT+GGG 0.719461 8.4:-12183500 None:intergenic
GAGAATCACGAATTGTAGGG+GGG 0.721144 8.4:-12183762 None:intergenic
CTATTAAAGCTATATCAGGG+AGG 0.766585 8.4:+12184336 MS.gene036495:CDS
ACGGTGGCAACAATGTCTTG+GGG 0.780918 8.4:-12183622 None:intergenic
GTCTCCGGACAAACTCAATG+CGG 0.781187 8.4:+12184080 MS.gene036495:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACACAAAAATTGTATTTGGA+AGG - chr8.4:12183931-12183950 None:intergenic 25.0%
! ATGCTATTAAAGCTATATCA+GGG + chr8.4:12184333-12184352 MS.gene036495:CDS 25.0%
! TATACTAAATCCGAAAAGAA+CGG - chr8.4:12184163-12184182 None:intergenic 25.0%
! TCAAGCAAAAATTGAAGATT+TGG - chr8.4:12183796-12183815 None:intergenic 25.0%
! TGAAGAAAAAGGTAACATTT+CGG - chr8.4:12183304-12183323 None:intergenic 25.0%
! TTCTTACCAATTCAAAAGAA+GGG - chr8.4:12183696-12183715 None:intergenic 25.0%
!! CTTCAATTTTTGCTTGATTT+TGG + chr8.4:12183799-12183818 MS.gene036495:CDS 25.0%
!! TTCCAAATACAATTTTTGTG+TGG + chr8.4:12183930-12183949 MS.gene036495:CDS 25.0%
!!! TGTTCTGAAGATGATATTTT+GGG + chr8.4:12184024-12184043 MS.gene036495:CDS 25.0%
!!! TTTGGCAAAATTGATTTGAT+GGG - chr8.4:12183571-12183590 None:intergenic 25.0%
AACTCGTCCTTCAAATAAAA+TGG - chr8.4:12184270-12184289 None:intergenic 30.0%
AAGAAAAAGGTAACATTTCG+GGG - chr8.4:12183302-12183321 None:intergenic 30.0%
ATACTAAATCCGAAAAGAAC+GGG - chr8.4:12184162-12184181 None:intergenic 30.0%
ATTGTTGATCTGAATGTGAA+AGG - chr8.4:12183357-12183376 None:intergenic 30.0%
CATGCTATTAAAGCTATATC+AGG + chr8.4:12184332-12184351 MS.gene036495:CDS 30.0%
CTTCTTACCAATTCAAAAGA+AGG - chr8.4:12183697-12183716 None:intergenic 30.0%
GAAGAAAAAGGTAACATTTC+GGG - chr8.4:12183303-12183322 None:intergenic 30.0%
GAGGCTAAAAATTGAAACTT+TGG - chr8.4:12183589-12183608 None:intergenic 30.0%
TCGGATTTAGTATAGAGAAA+AGG + chr8.4:12184169-12184188 MS.gene036495:CDS 30.0%
TGCCACACAAAAATTGTATT+TGG - chr8.4:12183935-12183954 None:intergenic 30.0%
TTGTTGATCTGAATGTGAAA+GGG - chr8.4:12183356-12183375 None:intergenic 30.0%
! CAATTTTTGTGTGGCATTAT+TGG + chr8.4:12183939-12183958 MS.gene036495:CDS 30.0%
! CATAATCCCTTCTTTTGAAT+TGG + chr8.4:12183687-12183706 MS.gene036495:CDS 30.0%
!! AACTGCACCATTTTATTTGA+AGG + chr8.4:12184260-12184279 MS.gene036495:CDS 30.0%
!! ATGCTCTACTGATTTGAAAA+AGG + chr8.4:12183873-12183892 MS.gene036495:CDS 30.0%
!! CAAAAGTATGTGAAACGTTT+TGG + chr8.4:12184297-12184316 MS.gene036495:CDS 30.0%
!! CTGATATAGCTTTAATAGCA+TGG - chr8.4:12184334-12184353 None:intergenic 30.0%
!! CTTTGGCAAAATTGATTTGA+TGG - chr8.4:12183572-12183591 None:intergenic 30.0%
!! GTAATAGCTTTATGATCAGA+AGG - chr8.4:12183727-12183746 None:intergenic 30.0%
!! TACTGATTTGAAAAAGGCTT+TGG + chr8.4:12183879-12183898 MS.gene036495:CDS 30.0%
!! TTGGCAAAATTGATTTGATG+GGG - chr8.4:12183570-12183589 None:intergenic 30.0%
!!! GTGTTCTGAAGATGATATTT+TGG + chr8.4:12184023-12184042 MS.gene036495:CDS 30.0%
!!! TTTCAATTTTTAGCCTCCAA+AGG + chr8.4:12183592-12183611 MS.gene036495:CDS 30.0%
ATCAGTAGAGCATATTGTAG+AGG - chr8.4:12183866-12183885 None:intergenic 35.0%
CTATTAAAGCTATATCAGGG+AGG + chr8.4:12184336-12184355 MS.gene036495:CDS 35.0%
GACAACCTAACTTAATGCTA+CGG + chr8.4:12184058-12184077 MS.gene036495:CDS 35.0%
GTATCTTCTTTCCAAAGGTT+TGG + chr8.4:12184218-12184237 MS.gene036495:CDS 35.0%
TCAGTAGAGCATATTGTAGA+GGG - chr8.4:12183865-12183884 None:intergenic 35.0%
TGGAGGAGATTAAGGAATTA+GGG + chr8.4:12183899-12183918 MS.gene036495:CDS 35.0%
TTGGAGGAGATTAAGGAATT+AGG + chr8.4:12183898-12183917 MS.gene036495:CDS 35.0%
TTGGGTCAAACAATTTGGTT+GGG + chr8.4:12184113-12184132 MS.gene036495:CDS 35.0%
TTTGGGTCAAACAATTTGGT+TGG + chr8.4:12184112-12184131 MS.gene036495:CDS 35.0%
! AACCTTTGGAAAGAAGATAC+TGG - chr8.4:12184218-12184237 None:intergenic 35.0%
! AACGTTTCACATACTTTTGC+AGG - chr8.4:12184296-12184315 None:intergenic 35.0%
! TGATTTGAAAAAGGCTTTGG+AGG + chr8.4:12183882-12183901 MS.gene036495:CDS 35.0%
!!! CGTTTTTGGGTCAAACAATT+TGG + chr8.4:12184108-12184127 MS.gene036495:CDS 35.0%
!!! GGTTTTGAAAAAGGCGATAA+CGG - chr8.4:12183482-12183501 None:intergenic 35.0%
AAAGGTAACATTTCGGGGTT+TGG - chr8.4:12183297-12183316 None:intergenic 40.0%
AAGCGAGAATCACGAATTGT+AGG - chr8.4:12183769-12183788 None:intergenic 40.0%
AAGGGATTATGTACTTCTCG+AGG - chr8.4:12183678-12183697 None:intergenic 40.0%
AAGGTAACATTTCGGGGTTT+GGG - chr8.4:12183296-12183315 None:intergenic 40.0%
AGAGAAAAGGCTTATTCCGA+GGG + chr8.4:12184182-12184201 MS.gene036495:CDS 40.0%
AGATACAAACCATCATTCGC+AGG + chr8.4:12183521-12183540 MS.gene036495:CDS 40.0%
AGCGAGAATCACGAATTGTA+GGG - chr8.4:12183768-12183787 None:intergenic 40.0%
AGGGTCTAGTAGTAAGCAAA+TGG - chr8.4:12183846-12183865 None:intergenic 40.0%
ATCAACTTTGGCATCCCATT+GGG - chr8.4:12183986-12184005 None:intergenic 40.0%
ATGTTTCATTATCACCGCCT+CGG + chr8.4:12183256-12183275 MS.gene036495:CDS 40.0%
GTCCAGTATCTTCTTTCCAA+AGG + chr8.4:12184213-12184232 MS.gene036495:CDS 40.0%
TAACTTAATGCTACGGTCTC+CGG + chr8.4:12184065-12184084 MS.gene036495:CDS 40.0%
TAGAGAAAAGGCTTATTCCG+AGG + chr8.4:12184181-12184200 MS.gene036495:CDS 40.0%
TGTGGCATTATTGGCCAAAA+GGG + chr8.4:12183948-12183967 MS.gene036495:CDS 40.0%
TTAAAGCTATATCAGGGAGG+AGG + chr8.4:12184339-12184358 MS.gene036495:CDS 40.0%
TTGATCTGAATGTGAAAGGG+TGG - chr8.4:12183353-12183372 None:intergenic 40.0%
! ACTCTTCAAGGCATCAACTT+TGG - chr8.4:12183998-12184017 None:intergenic 40.0%
! AGTTGATGCCTTGAAGAGTT+GGG + chr8.4:12183999-12184018 MS.gene036495:CDS 40.0%
! GATGGTTTGTATCTGGTGAT+CGG - chr8.4:12183515-12183534 None:intergenic 40.0%
! TGCGAATGATGGTTTGTATC+TGG - chr8.4:12183522-12183541 None:intergenic 40.0%
!! AAAGGCTTTGGAGGAGATTA+AGG + chr8.4:12183891-12183910 MS.gene036495:CDS 40.0%
!! AAGTTGATGCCTTGAAGAGT+TGG + chr8.4:12183998-12184017 MS.gene036495:CDS 40.0%
!! GTTTGTTGGGAGTGTTGAAA+CGG - chr8.4:12183453-12183472 None:intergenic 40.0%
!! TCTTTCCAAAGGTTTGGTGA+AGG + chr8.4:12184224-12184243 MS.gene036495:CDS 40.0%
!!! ATCGCCTTTTTCAAAACCCA+CGG + chr8.4:12183484-12183503 MS.gene036495:CDS 40.0%
!!! GAAACCGTGGGTTTTGAAAA+AGG - chr8.4:12183491-12183510 None:intergenic 40.0%
AACGGTGGCAACAATGTCTT+GGG - chr8.4:12183626-12183645 None:intergenic 45.0%
AGGTAACATTTCGGGGTTTG+GGG - chr8.4:12183295-12183314 None:intergenic 45.0%
ATAAACCGCAGTTGTTGGTG+AGG - chr8.4:12183396-12183415 None:intergenic 45.0%
ATCACCGCATTGAGTTTGTC+CGG - chr8.4:12184087-12184106 None:intergenic 45.0%
ATCCTCCTTCACCAAACCTT+TGG - chr8.4:12184232-12184251 None:intergenic 45.0%
ATGTGAAAGGGTGGAGAAGT+AGG - chr8.4:12183344-12183363 None:intergenic 45.0%
CATCAACTTTGGCATCCCAT+TGG - chr8.4:12183987-12184006 None:intergenic 45.0%
CGAGAATCACGAATTGTAGG+GGG - chr8.4:12183766-12183785 None:intergenic 45.0%
GAGAATCACGAATTGTAGGG+GGG - chr8.4:12183765-12183784 None:intergenic 45.0%
GCGAGAATCACGAATTGTAG+GGG - chr8.4:12183767-12183786 None:intergenic 45.0%
GGAGAAGTAGGTGAAAGAGT+GGG - chr8.4:12183332-12183351 None:intergenic 45.0%
GGAGACCGTAGCATTAAGTT+AGG - chr8.4:12184066-12184085 None:intergenic 45.0%
GTGTGGCATTATTGGCCAAA+AGG + chr8.4:12183947-12183966 MS.gene036495:CDS 45.0%
GTTGATGCCTTGAAGAGTTG+GGG + chr8.4:12184000-12184019 MS.gene036495:CDS 45.0%
TAACGGTGGCAACAATGTCT+TGG - chr8.4:12183627-12183646 None:intergenic 45.0%
TGGAGAAGTAGGTGAAAGAG+TGG - chr8.4:12183333-12183352 None:intergenic 45.0%
TGGAGATAAACCGCAGTTGT+TGG - chr8.4:12183401-12183420 None:intergenic 45.0%
TGGGATCCTTTATTGCTCCT+GGG + chr8.4:12184132-12184151 MS.gene036495:CDS 45.0%
TTGATGCCTTGAAGAGTTGG+GGG + chr8.4:12184001-12184020 MS.gene036495:CDS 45.0%
TTGGGATCCTTTATTGCTCC+TGG + chr8.4:12184131-12184150 MS.gene036495:CDS 45.0%
! AGTAGGTGAAAGAGTGGGTT+CGG - chr8.4:12183327-12183346 None:intergenic 45.0%
! AGTGTTGAAACGGAGTCGTT+TGG - chr8.4:12183443-12183462 None:intergenic 45.0%
! ATAACGGAGTCGGGTTTGTT+GGG - chr8.4:12183466-12183485 None:intergenic 45.0%
! CAATGCGGTGATGCGTTTTT+GGG + chr8.4:12184095-12184114 MS.gene036495:CDS 45.0%
! TCAATGCGGTGATGCGTTTT+TGG + chr8.4:12184094-12184113 MS.gene036495:CDS 45.0%
! TGTTGGTGAGGTAGGAAACT+GGG - chr8.4:12183384-12183403 None:intergenic 45.0%
! TTGTTGGTGAGGTAGGAAAC+TGG - chr8.4:12183385-12183404 None:intergenic 45.0%
!! AAAAAGGCGATAACGGAGTC+GGG - chr8.4:12183475-12183494 None:intergenic 45.0%
!! AGTGGGTTCGGTGAAGAAAA+AGG - chr8.4:12183315-12183334 None:intergenic 45.0%
!! GAAAAAGGCGATAACGGAGT+CGG - chr8.4:12183476-12183495 None:intergenic 45.0%
!! TTCCAAAGGTTTGGTGAAGG+AGG + chr8.4:12184227-12184246 MS.gene036495:CDS 45.0%
ACGGTGGCAACAATGTCTTG+GGG - chr8.4:12183625-12183644 None:intergenic 50.0%
ACTTCTCGAGGCTTGAAGCA+AGG - chr8.4:12183666-12183685 None:intergenic 50.0%
AGAACACCCCCAACTCTTCA+AGG - chr8.4:12184010-12184029 None:intergenic 50.0%
AGGCTTGAAGCAAGGAAACG+GGG - chr8.4:12183658-12183677 None:intergenic 50.0%
CGAGGCTTGAAGCAAGGAAA+CGG - chr8.4:12183660-12183679 None:intergenic 50.0%
CGATGGAATCCTGCGAATGA+TGG - chr8.4:12183533-12183552 None:intergenic 50.0%
GAAACGGGGGCTTCTAGTAA+CGG - chr8.4:12183644-12183663 None:intergenic 50.0%
GAGGCTTGAAGCAAGGAAAC+GGG - chr8.4:12183659-12183678 None:intergenic 50.0%
GGTAACATTTCGGGGTTTGG+GGG - chr8.4:12183294-12183313 None:intergenic 50.0%
GTCTCCGGACAAACTCAATG+CGG + chr8.4:12184080-12184099 MS.gene036495:CDS 50.0%
TACTGGACAACAGAAGCCCT+CGG - chr8.4:12184201-12184220 None:intergenic 50.0%
TCCTACCTCACCAACAACTG+CGG + chr8.4:12183388-12183407 MS.gene036495:CDS 50.0%
TTTGGCATCCCATTGGGACT+TGG - chr8.4:12183980-12183999 None:intergenic 50.0%
! GGGTTTGAAACGATGAGCGA+TGG - chr8.4:12183550-12183569 None:intergenic 50.0%
!! GATAACGGAGTCGGGTTTGT+TGG - chr8.4:12183467-12183486 None:intergenic 50.0%
!! GATGCTTTGAGAGCGTCTTG+TGG - chr8.4:12183421-12183440 None:intergenic 50.0%
CTGGTGATCGGAGAAACCGT+GGG - chr8.4:12183503-12183522 None:intergenic 55.0%
GGCTGTCACCAAGTCCCAAT+GGG + chr8.4:12183969-12183988 MS.gene036495:CDS 55.0%
GGCTTGAAGCAAGGAAACGG+GGG - chr8.4:12183657-12183676 None:intergenic 55.0%
GGTGTGCCCAGGAGCAATAA+AGG - chr8.4:12184141-12184160 None:intergenic 55.0%
TCTGGTGATCGGAGAAACCG+TGG - chr8.4:12183504-12183523 None:intergenic 55.0%
! ACCGCAGTTGTTGGTGAGGT+AGG - chr8.4:12183392-12183411 None:intergenic 55.0%
! CTGGGCACACCCGTTCTTTT+CGG + chr8.4:12184150-12184169 MS.gene036495:CDS 55.0%
!! GTCTTGGGGAGAAGCACCTT+TGG - chr8.4:12183611-12183630 None:intergenic 55.0%
!! TTGGGGAGAAGCACCTTTGG+AGG - chr8.4:12183608-12183627 None:intergenic 55.0%
!!! GGACTTGGTGACAGCCCTTT+TGG - chr8.4:12183965-12183984 None:intergenic 55.0%
ACGGGGGCTTCTAGTAACGG+TGG - chr8.4:12183641-12183660 None:intergenic 60.0%
CGAAAAGAACGGGTGTGCCC+AGG - chr8.4:12184152-12184171 None:intergenic 60.0%
GGGCTGTCACCAAGTCCCAA+TGG + chr8.4:12183968-12183987 MS.gene036495:CDS 60.0%
GGTTGAGAAGAGCTCCGAGG+CGG - chr8.4:12183273-12183292 None:intergenic 60.0%
GGGGGTTGAGAAGAGCTCCG+AGG - chr8.4:12183276-12183295 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 12183256 12184368 12183256 ID=MS.gene036495
chr8.4 mRNA 12183256 12184368 12183256 ID=MS.gene036495.t1;Parent=MS.gene036495
chr8.4 exon 12183256 12184368 12183256 ID=MS.gene036495.t1.exon1;Parent=MS.gene036495.t1
chr8.4 CDS 12183256 12184368 12183256 ID=cds.MS.gene036495.t1;Parent=MS.gene036495.t1
Gene Sequence

>MS.gene036495

ATGTTTCATTATCACCGCCTCGGAGCTCTTCTCAACCCCCAAACCCCGAAATGTTACCTTTTTCTTCACCGAACCCACTCTTTCACCTACTTCTCCACCCTTTCACATTCAGATCAACAATGTTTCCCAGTTTCCTACCTCACCAACAACTGCGGTTTATCTCCACAAGACGCTCTCAAAGCATCCAAACGACTCCGTTTCAACACTCCCAACAAACCCGACTCCGTTATCGCCTTTTTCAAAACCCACGGTTTCTCCGATCACCAGATACAAACCATCATTCGCAGGATTCCATCGCTCATCGTTTCAAACCCCATCAAATCAATTTTGCCAAAGTTTCAATTTTTAGCCTCCAAAGGTGCTTCTCCCCAAGACATTGTTGCCACCGTTACTAGAAGCCCCCGTTTCCTTGCTTCAAGCCTCGAGAAGTACATAATCCCTTCTTTTGAATTGGTAAGAAGTTTTTGTCCTTCTGATCATAAAGCTATTACTTCAATTATCGTTTGCCCCCCTACAATTCGTGATTCTCGCTTGAAACCAAATCTTCAATTTTTGCTTGATTTTGGTGTCACTCGTTCAAGTATTTACCATTTGCTTACTACTAGACCCTCTACAATATGCTCTACTGATTTGAAAAAGGCTTTGGAGGAGATTAAGGAATTAGGGTTTCAACCTTCCAAATACAATTTTTGTGTGGCATTATTGGCCAAAAGGGCTGTCACCAAGTCCCAATGGGATGCCAAAGTTGATGCCTTGAAGAGTTGGGGGTGTTCTGAAGATGATATTTTGGGTGCATTTAAAAGACAACCTAACTTAATGCTACGGTCTCCGGACAAACTCAATGCGGTGATGCGTTTTTGGGTCAAACAATTTGGTTGGGATCCTTTATTGCTCCTGGGCACACCCGTTCTTTTCGGATTTAGTATAGAGAAAAGGCTTATTCCGAGGGCTTCTGTTGTCCAGTATCTTCTTTCCAAAGGTTTGGTGAAGGAGGATGCTAGCTTAACTGCACCATTTTATTTGAAGGACGAGTTGTTCCTGCAAAAGTATGTGAAACGTTTTGGAGACGAAGCTTCCATGCTATTAAAGCTATATCAGGGAGGAGGATGCTAG
Protein sequence

>MS.gene036495.t1

MFHYHRLGALLNPQTPKCYLFLHRTHSFTYFSTLSHSDQQCFPVSYLTNNCGLSPQDALKASKRLRFNTPNKPDSVIAFFKTHGFSDHQIQTIIRRIPSLIVSNPIKSILPKFQFLASKGASPQDIVATVTRSPRFLASSLEKYIIPSFELVRSFCPSDHKAITSIIVCPPTIRDSRLKPNLQFLLDFGVTRSSIYHLLTTRPSTICSTDLKKALEEIKELGFQPSKYNFCVALLAKRAVTKSQWDAKVDALKSWGCSEDDILGAFKRQPNLMLRSPDKLNAVMRFWVKQFGWDPLLLLGTPVLFGFSIEKRLIPRASVVQYLLSKGLVKEDASLTAPFYLKDELFLQKYVKRFGDEASMLLKLYQGGGC