Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036533.t1 | XP_003609586.1 | 91.8 | 170 | 14 | 0 | 1 | 170 | 12 | 181 | 1.30E-73 | 285.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036533.t1 | G7JJ33 | 91.8 | 170 | 14 | 0 | 1 | 170 | 12 | 181 | 9.4e-74 | 285.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene036533.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036533.t1 | MTR_4g118830 | 91.765 | 170 | 14 | 0 | 1 | 170 | 12 | 181 | 1.56e-109 | 310 |
| MS.gene036533.t1 | MTR_4g118430 | 45.946 | 111 | 41 | 3 | 66 | 170 | 1 | 98 | 2.32e-21 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTCTCTGTTACGGTTAC+TGG | 0.183266 | 8.4:-12676646 | MS.gene036533:CDS |
| AGTAACCGTAACAGAGAAAT+TGG | 0.195649 | 8.4:+12676648 | None:intergenic |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.199938 | 8.4:-12676622 | MS.gene036533:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8.4:-12676675 | MS.gene036533:CDS |
| GAGAGAGATTATGAGTTTGT+AGG | 0.295578 | 8.4:-12676437 | None:intergenic |
| CAAGCAATGTGGCTTTCTTT+GGG | 0.307847 | 8.4:-12676621 | MS.gene036533:CDS |
| ATCACTGCTTTGGTTCAAAA+TGG | 0.315294 | 8.4:-12676481 | MS.gene036533:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8.4:-12676491 | MS.gene036533:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8.4:+12676963 | None:intergenic |
| GAATCCCAATTTCTCTGTTA+CGG | 0.433893 | 8.4:-12676653 | MS.gene036533:CDS |
| TAATGATGGTAATTTGATCT+TGG | 0.443216 | 8.4:-12676506 | MS.gene036533:CDS |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8.4:-12676737 | MS.gene036533:CDS |
| TGAAGAGAAGAAGAAAATGT+TGG | 0.449297 | 8.4:-12676749 | MS.gene036533:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8.4:+12676962 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8.4:-12676707 | MS.gene036533:CDS |
| GCTAATGTTTCTGTTAATGA+TGG | 0.496957 | 8.4:-12676520 | MS.gene036533:CDS |
| TTCAACGTTTAGAACCTCTT+GGG | 0.500668 | 8.4:+12677039 | None:intergenic |
| TTTCTTTGGGATTGAATCTG+TGG | 0.501659 | 8.4:-12676608 | MS.gene036533:CDS |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8.4:+12676978 | None:intergenic |
| GTTCAACGTTTAGAACCTCT+TGG | 0.509820 | 8.4:+12677038 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8.4:-12676598 | MS.gene036533:CDS |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8.4:-12676709 | MS.gene036533:CDS |
| AGAGAAGAAGAAAATGTTGG+AGG | 0.534106 | 8.4:-12676746 | MS.gene036533:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8.4:-12676708 | MS.gene036533:CDS |
| GTAACCGTAACAGAGAAATT+GGG | 0.551700 | 8.4:+12676649 | None:intergenic |
| TAAACGTTGAACGTGAAAGA+AGG | 0.567677 | 8.4:-12677027 | MS.gene036533:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8.4:-12676706 | MS.gene036533:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8.4:+12676580 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8.4:-12676632 | MS.gene036533:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8.4:+12676970 | None:intergenic |
| TTGGGATTCCTATTACGACA+CGG | 0.678110 | 8.4:+12676667 | None:intergenic |
| CACTACTACTAAAACCCAAG+AGG | 0.682406 | 8.4:-12677053 | MS.gene036533:CDS |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8.4:-12677076 | MS.gene036533:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAATAAAAGAGAAAA+TGG | + | chr8.4:12676589-12676608 | None:intergenic | 15.0% |
| !! | AAAATAAAAGAGAAAATGGA+AGG | + | chr8.4:12676585-12676604 | None:intergenic | 20.0% |
| !! | ATAACAAATCAAACCATAAT+GGG | + | chr8.4:12676669-12676688 | None:intergenic | 20.0% |
| !!! | TTTGATTTGTTATCTGAAAT+GGG | - | chr8.4:12676676-12676695 | MS.gene036533:CDS | 20.0% |
| ! | GATAACAAATCAAACCATAA+TGG | + | chr8.4:12676670-12676689 | None:intergenic | 25.0% |
| ! | TAATGATGGTAATTTGATCT+TGG | - | chr8.4:12677005-12677024 | MS.gene036533:CDS | 25.0% |
| ! | TCATTCTTCATACTTCATTT+GGG | - | chr8.4:12676604-12676623 | MS.gene036533:CDS | 25.0% |
| ! | TTCATTCTTCATACTTCATT+TGG | - | chr8.4:12676603-12676622 | MS.gene036533:CDS | 25.0% |
| !! | TTTGATTACTGTGATTTTGA+AGG | - | chr8.4:12676936-12676955 | MS.gene036533:intron | 25.0% |
| !!! | GTTTGATTTGTTATCTGAAA+TGG | - | chr8.4:12676675-12676694 | MS.gene036533:CDS | 25.0% |
| !!! | TGAAATGGGTTTTTGTTTTT+AGG | - | chr8.4:12676690-12676709 | MS.gene036533:CDS | 25.0% |
| AAAATCACAGTAATCAAACC+AGG | + | chr8.4:12676934-12676953 | None:intergenic | 30.0% | |
| AAATCAAAGAAACCTGTTGA+AGG | - | chr8.4:12676802-12676821 | MS.gene036533:CDS | 30.0% | |
| AATATTTGATCGACCCATTA+TGG | - | chr8.4:12676653-12676672 | MS.gene036533:CDS | 30.0% | |
| AATCAAAGAAACCTGTTGAA+GGG | - | chr8.4:12676803-12676822 | MS.gene036533:intron | 30.0% | |
| AGGAATGATGTTAGTTACAT+TGG | + | chr8.4:12676565-12676584 | None:intergenic | 30.0% | |
| GAATGATGTTAGTTACATTG+GGG | + | chr8.4:12676563-12676582 | None:intergenic | 30.0% | |
| GGAATGATGTTAGTTACATT+GGG | + | chr8.4:12676564-12676583 | None:intergenic | 30.0% | |
| TGAAGAGAAGAAGAAAATGT+TGG | - | chr8.4:12676762-12676781 | MS.gene036533:CDS | 30.0% | |
| ! | GCTAATGTTTCTGTTAATGA+TGG | - | chr8.4:12676991-12677010 | MS.gene036533:CDS | 30.0% |
| !!! | TTTTTGTTTTTAGGCTACAC+AGG | - | chr8.4:12676699-12676718 | MS.gene036533:CDS | 30.0% |
| AAATTAAAACCCCCTTCAAC+AGG | + | chr8.4:12676817-12676836 | None:intergenic | 35.0% | |
| AATTTCTCTGTTACGGTTAC+TGG | - | chr8.4:12676865-12676884 | MS.gene036533:intron | 35.0% | |
| AGAGAAGAAGAAAATGTTGG+AGG | - | chr8.4:12676765-12676784 | MS.gene036533:CDS | 35.0% | |
| AGTAACCGTAACAGAGAAAT+TGG | + | chr8.4:12676866-12676885 | None:intergenic | 35.0% | |
| ATCAAAGAAACCTGTTGAAG+GGG | - | chr8.4:12676804-12676823 | MS.gene036533:intron | 35.0% | |
| GAATCCCAATTTCTCTGTTA+CGG | - | chr8.4:12676858-12676877 | MS.gene036533:intron | 35.0% | |
| GTAACCGTAACAGAGAAATT+GGG | + | chr8.4:12676865-12676884 | None:intergenic | 35.0% | |
| TAAACGTTGAACGTGAAAGA+AGG | - | chr8.4:12676484-12676503 | MS.gene036533:CDS | 35.0% | |
| ! | ATCACTGCTTTGGTTCAAAA+TGG | - | chr8.4:12677030-12677049 | MS.gene036533:CDS | 35.0% |
| ! | TTCAACGTTTAGAACCTCTT+GGG | + | chr8.4:12676475-12676494 | None:intergenic | 35.0% |
| !! | GTTTTTGAAGAATGAAGCTG+AGG | - | chr8.4:12676960-12676979 | MS.gene036533:intron | 35.0% |
| !! | TTTCTTTGGGATTGAATCTG+TGG | - | chr8.4:12676903-12676922 | MS.gene036533:intron | 35.0% |
| !!! | TGAAGGGGGTTTTAATTTGT+TGG | - | chr8.4:12676819-12676838 | MS.gene036533:intron | 35.0% |
| ATTGAATCTGTGGCTAAACC+TGG | - | chr8.4:12676913-12676932 | MS.gene036533:intron | 40.0% | |
| CAAGCAATGTGGCTTTCTTT+GGG | - | chr8.4:12676890-12676909 | MS.gene036533:intron | 40.0% | |
| CACTACTACTAAAACCCAAG+AGG | - | chr8.4:12676458-12676477 | MS.gene036533:CDS | 40.0% | |
| GAAAATGTTGGAGGAGATGA+AGG | - | chr8.4:12676774-12676793 | MS.gene036533:CDS | 40.0% | |
| TCAAAGAAACCTGTTGAAGG+GGG | - | chr8.4:12676805-12676824 | MS.gene036533:intron | 40.0% | |
| TCAAGCAATGTGGCTTTCTT+TGG | - | chr8.4:12676889-12676908 | MS.gene036533:intron | 40.0% | |
| TTACATTGGGGTAGAGAGTT+GGG | + | chr8.4:12676551-12676570 | None:intergenic | 40.0% | |
| TTGGGATTCCTATTACGACA+CGG | + | chr8.4:12676847-12676866 | None:intergenic | 40.0% | |
| ! | GTTCAACGTTTAGAACCTCT+TGG | + | chr8.4:12676476-12676495 | None:intergenic | 40.0% |
| GTTACATTGGGGTAGAGAGT+TGG | + | chr8.4:12676552-12676571 | None:intergenic | 45.0% | |
| !! | GGTTACTGGTTCAAGCAATG+TGG | - | chr8.4:12676879-12676898 | MS.gene036533:intron | 45.0% |
| !! | TGTTGGTTCCGTGTCGTAAT+AGG | - | chr8.4:12676836-12676855 | MS.gene036533:intron | 45.0% |
| GATCTTGGCGATCACTGCTT+TGG | - | chr8.4:12677020-12677039 | MS.gene036533:CDS | 50.0% | |
| ! | GAGTTGGGACAGTGGTAGTG+AGG | + | chr8.4:12676536-12676555 | None:intergenic | 55.0% |
| GGGGTAGAGAGTTGGGACAG+TGG | + | chr8.4:12676544-12676563 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 12676438 | 12677095 | 12676438 | ID=MS.gene036533 |
| chr8.4 | mRNA | 12676438 | 12677095 | 12676438 | ID=MS.gene036533.t1;Parent=MS.gene036533 |
| chr8.4 | exon | 12676967 | 12677095 | 12676967 | ID=MS.gene036533.t1.exon1;Parent=MS.gene036533.t1 |
| chr8.4 | CDS | 12676967 | 12677095 | 12676967 | ID=cds.MS.gene036533.t1;Parent=MS.gene036533.t1 |
| chr8.4 | exon | 12676438 | 12676821 | 12676438 | ID=MS.gene036533.t1.exon2;Parent=MS.gene036533.t1 |
| chr8.4 | CDS | 12676438 | 12676821 | 12676438 | ID=cds.MS.gene036533.t1;Parent=MS.gene036533.t1 |
| Gene Sequence |
| Protein sequence |
