Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036665.t1 | XP_003609491.2 | 97.7 | 129 | 3 | 0 | 1 | 129 | 4 | 132 | 5.50E-72 | 280 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036665.t1 | G7JGM8 | 97.7 | 129 | 3 | 0 | 1 | 129 | 2 | 130 | 3.9e-72 | 280.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene036665.t1 | TF | PLATZ |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036665.t1 | MTR_4g116300 | 97.674 | 129 | 3 | 0 | 1 | 129 | 2 | 130 | 6.08e-93 | 268 |
| MS.gene036665.t1 | MTR_4g094680 | 62.687 | 134 | 44 | 1 | 2 | 129 | 19 | 152 | 4.14e-58 | 180 |
| MS.gene036665.t1 | MTR_2g036170 | 57.143 | 126 | 48 | 1 | 4 | 129 | 36 | 155 | 3.84e-46 | 150 |
| MS.gene036665.t1 | MTR_2g055210 | 52.344 | 128 | 60 | 1 | 2 | 129 | 99 | 225 | 6.75e-45 | 149 |
| MS.gene036665.t1 | MTR_5g007230 | 48.413 | 126 | 64 | 1 | 5 | 129 | 21 | 146 | 5.32e-40 | 133 |
| MS.gene036665.t1 | MTR_4g078170 | 49.206 | 126 | 63 | 1 | 5 | 129 | 15 | 140 | 2.55e-39 | 131 |
| MS.gene036665.t1 | MTR_8g066820 | 48.837 | 129 | 65 | 1 | 2 | 129 | 30 | 158 | 5.13e-39 | 131 |
| MS.gene036665.t1 | MTR_5g030130 | 48.062 | 129 | 66 | 1 | 2 | 129 | 32 | 160 | 2.37e-37 | 125 |
| MS.gene036665.t1 | MTR_5g030130 | 48.062 | 129 | 66 | 1 | 2 | 129 | 32 | 160 | 4.13e-37 | 126 |
| MS.gene036665.t1 | MTR_7g102790 | 44.186 | 129 | 68 | 1 | 5 | 129 | 7 | 135 | 1.92e-33 | 116 |
| MS.gene036665.t1 | MTR_1g062160 | 42.520 | 127 | 71 | 2 | 5 | 129 | 7 | 133 | 2.83e-30 | 108 |
| MS.gene036665.t1 | MTR_2g084525 | 39.259 | 135 | 72 | 4 | 5 | 129 | 5 | 139 | 5.45e-24 | 92.8 |
| MS.gene036665.t1 | MTR_2g084495 | 38.806 | 134 | 71 | 4 | 7 | 129 | 6 | 139 | 1.64e-21 | 86.3 |
| MS.gene036665.t1 | MTR_8g105930 | 34.028 | 144 | 73 | 3 | 7 | 129 | 15 | 157 | 3.44e-21 | 85.5 |
| MS.gene036665.t1 | MTR_2g084510 | 34.815 | 135 | 78 | 4 | 5 | 129 | 4 | 138 | 7.54e-20 | 82.0 |
| MS.gene036665.t1 | MTR_2g060500 | 37.313 | 134 | 73 | 5 | 7 | 129 | 12 | 145 | 2.56e-19 | 78.6 |
| MS.gene036665.t1 | MTR_2g060540 | 36.567 | 134 | 74 | 5 | 7 | 129 | 12 | 145 | 7.53e-19 | 77.4 |
| MS.gene036665.t1 | MTR_8g097310 | 34.343 | 99 | 57 | 2 | 33 | 129 | 2 | 94 | 3.33e-13 | 62.8 |
| MS.gene036665.t1 | MTR_5g077740 | 31.868 | 91 | 59 | 2 | 4 | 92 | 22 | 111 | 1.61e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036665.t1 | AT2G12646 | 87.597 | 129 | 16 | 0 | 1 | 129 | 2 | 130 | 1.54e-84 | 248 |
| MS.gene036665.t1 | AT2G12646 | 87.597 | 129 | 16 | 0 | 1 | 129 | 2 | 130 | 1.54e-84 | 248 |
| MS.gene036665.t1 | AT2G12646 | 88.095 | 126 | 15 | 0 | 4 | 129 | 2 | 127 | 4.02e-83 | 244 |
| MS.gene036665.t1 | AT3G60670 | 63.200 | 125 | 46 | 0 | 5 | 129 | 7 | 131 | 6.41e-58 | 179 |
| MS.gene036665.t1 | AT1G31040 | 66.667 | 126 | 41 | 1 | 4 | 129 | 18 | 142 | 4.62e-56 | 175 |
| MS.gene036665.t1 | AT1G32700 | 50.000 | 126 | 62 | 1 | 5 | 129 | 12 | 137 | 6.16e-37 | 125 |
| MS.gene036665.t1 | AT4G17900 | 46.923 | 130 | 67 | 2 | 2 | 129 | 17 | 146 | 2.09e-36 | 124 |
| MS.gene036665.t1 | AT4G17900 | 46.923 | 130 | 67 | 2 | 2 | 129 | 31 | 160 | 2.45e-36 | 124 |
| MS.gene036665.t1 | AT1G21000 | 47.619 | 126 | 65 | 1 | 5 | 129 | 13 | 138 | 9.31e-35 | 120 |
| MS.gene036665.t1 | AT1G21000 | 47.619 | 126 | 65 | 1 | 5 | 129 | 16 | 141 | 1.02e-34 | 120 |
| MS.gene036665.t1 | AT1G76590 | 46.825 | 126 | 66 | 1 | 5 | 129 | 18 | 143 | 2.47e-34 | 119 |
| MS.gene036665.t1 | AT1G43000 | 43.651 | 126 | 70 | 1 | 5 | 129 | 16 | 141 | 9.09e-33 | 115 |
| MS.gene036665.t1 | AT1G43000 | 43.651 | 126 | 70 | 1 | 5 | 129 | 9 | 134 | 9.48e-33 | 114 |
| MS.gene036665.t1 | AT5G46710 | 41.538 | 130 | 74 | 2 | 2 | 129 | 25 | 154 | 1.63e-30 | 107 |
| MS.gene036665.t1 | AT5G46710 | 41.538 | 130 | 74 | 2 | 2 | 129 | 9 | 138 | 3.52e-30 | 108 |
| MS.gene036665.t1 | AT5G46710 | 41.538 | 130 | 74 | 2 | 2 | 129 | 25 | 154 | 6.34e-30 | 107 |
| MS.gene036665.t1 | AT2G01818 | 41.045 | 134 | 69 | 3 | 6 | 129 | 12 | 145 | 1.18e-28 | 104 |
| MS.gene036665.t1 | AT2G27930 | 41.304 | 138 | 70 | 3 | 3 | 129 | 2 | 139 | 3.35e-28 | 101 |
| MS.gene036665.t1 | AT2G27930 | 41.304 | 138 | 70 | 3 | 3 | 129 | 2 | 139 | 3.35e-28 | 101 |
| MS.gene036665.t1 | AT2G27930 | 41.304 | 138 | 70 | 3 | 3 | 129 | 2 | 139 | 5.51e-28 | 102 |
| MS.gene036665.t1 | AT2G27930 | 41.304 | 138 | 70 | 3 | 3 | 129 | 2 | 139 | 5.51e-28 | 102 |
| MS.gene036665.t1 | AT1G32700 | 51.042 | 96 | 46 | 1 | 35 | 129 | 3 | 98 | 1.20e-26 | 98.2 |
| MS.gene036665.t1 | AT2G27930 | 44.048 | 84 | 38 | 1 | 55 | 129 | 59 | 142 | 4.04e-17 | 73.9 |
| MS.gene036665.t1 | AT2G27930 | 44.048 | 84 | 38 | 1 | 55 | 129 | 59 | 142 | 4.04e-17 | 73.9 |
| MS.gene036665.t1 | AT2G01818 | 38.158 | 76 | 41 | 2 | 60 | 129 | 6 | 81 | 2.75e-12 | 60.5 |
Find 25 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAGAGAGCAATGGATAAA+AGG | 0.296836 | 8.1:-10732899 | None:intergenic |
| CTTCCAACCATGCAGGCTTT+TGG | 0.312977 | 8.1:-10732373 | None:intergenic |
| TATACAGAAAAGTTCTTTGT+TGG | 0.345795 | 8.1:+10732401 | MS.gene036665:CDS |
| AGCAATGGATAAAAGGTTCT+TGG | 0.405041 | 8.1:-10732892 | None:intergenic |
| TTATCATGATGTTCTTAGAT+TGG | 0.410755 | 8.1:+10732553 | MS.gene036665:CDS |
| TTCCTGATCCTTTGAATTGG+CGG | 0.427487 | 8.1:-10732841 | None:intergenic |
| AGTTTCCTGATCCTTTGAAT+TGG | 0.442272 | 8.1:-10732844 | None:intergenic |
| CTTCTTGATGAACACCACTT+TGG | 0.453482 | 8.1:-10732807 | None:intergenic |
| TTTGGCACTATTGATAGTAT+AGG | 0.459040 | 8.1:-10732789 | None:intergenic |
| AACCGCCAATTCAAAGGATC+AGG | 0.462617 | 8.1:+10732839 | MS.gene036665:CDS |
| AAACCAAAAGCCTGCATGGT+TGG | 0.480103 | 8.1:+10732370 | MS.gene036665:CDS |
| CCATTGCTCTCTTGGATGCA+AGG | 0.504085 | 8.1:+10732907 | MS.gene036665:CDS |
| CCTCAAAACCGCCAATTCAA+AGG | 0.532742 | 8.1:+10732833 | MS.gene036665:CDS |
| AGGCGGTGGTAGCGATGAGA+TGG | 0.543611 | 8.1:-10732509 | None:intergenic |
| AATGGATAAAAGGTTCTTGG+AGG | 0.548499 | 8.1:-10732889 | None:intergenic |
| TAAAGAGCTTCCAACCATGC+AGG | 0.552228 | 8.1:-10732380 | None:intergenic |
| TATAGACTGCACCAATGTCC+AGG | 0.557416 | 8.1:+10732592 | MS.gene036665:CDS |
| TACTATCAATAGTGCCAAAG+TGG | 0.558364 | 8.1:+10732793 | MS.gene036665:CDS |
| AGGGAAACCAAAAGCCTGCA+TGG | 0.579853 | 8.1:+10732366 | None:intergenic |
| CATAACGACGAACTTGAAGA+AGG | 0.581891 | 8.1:-10732529 | None:intergenic |
| CCTTGCATCCAAGAGAGCAA+TGG | 0.619111 | 8.1:-10732907 | None:intergenic |
| CGATGAGATGGAAAGCAATG+AGG | 0.624526 | 8.1:-10732497 | None:intergenic |
| GACGAACTTGAAGAAGGCGG+TGG | 0.658588 | 8.1:-10732523 | None:intergenic |
| ATGTATATGTACCTGGACAT+TGG | 0.663071 | 8.1:-10732603 | None:intergenic |
| AACGACGAACTTGAAGAAGG+CGG | 0.734968 | 8.1:-10732526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTACATAAGATTTAAT+TGG | - | chr8.1:10732711-10732730 | None:intergenic | 15.0% |
| !!! | TTTTAATTTCTGATTGAAAA+AGG | + | chr8.1:10732743-10732762 | MS.gene036665:intron | 15.0% |
| !! | AAATCTTATGTAATTCAACA+TGG | + | chr8.1:10732714-10732733 | MS.gene036665:intron | 20.0% |
| ! | TATACAGAAAAGTTCTTTGT+TGG | + | chr8.1:10732401-10732420 | MS.gene036665:CDS | 25.0% |
| ! | TTATCATGATGTTCTTAGAT+TGG | + | chr8.1:10732553-10732572 | MS.gene036665:CDS | 25.0% |
| !! | TTTGAATTATGTTGTTTACC+AGG | + | chr8.1:10732766-10732785 | MS.gene036665:intron | 25.0% |
| ATTGAACATGTATATGTACC+TGG | - | chr8.1:10732613-10732632 | None:intergenic | 30.0% | |
| ! | TTTGGCACTATTGATAGTAT+AGG | - | chr8.1:10732792-10732811 | None:intergenic | 30.0% |
| !!! | CATTCTTTTTTGCATTCTCA+TGG | - | chr8.1:10732436-10732455 | None:intergenic | 30.0% |
| AATGGATAAAAGGTTCTTGG+AGG | - | chr8.1:10732892-10732911 | None:intergenic | 35.0% | |
| AGCAATGGATAAAAGGTTCT+TGG | - | chr8.1:10732895-10732914 | None:intergenic | 35.0% | |
| AGTTTCCTGATCCTTTGAAT+TGG | - | chr8.1:10732847-10732866 | None:intergenic | 35.0% | |
| ATGTATATGTACCTGGACAT+TGG | - | chr8.1:10732606-10732625 | None:intergenic | 35.0% | |
| TACTATCAATAGTGCCAAAG+TGG | + | chr8.1:10732793-10732812 | MS.gene036665:CDS | 35.0% | |
| CATAACGACGAACTTGAAGA+AGG | - | chr8.1:10732532-10732551 | None:intergenic | 40.0% | |
| CCAAGAGAGCAATGGATAAA+AGG | - | chr8.1:10732902-10732921 | None:intergenic | 40.0% | |
| CTTCTTGATGAACACCACTT+TGG | - | chr8.1:10732810-10732829 | None:intergenic | 40.0% | |
| TTCCTGATCCTTTGAATTGG+CGG | - | chr8.1:10732844-10732863 | None:intergenic | 40.0% | |
| ! | CACTATTGATAGTATAGGCC+TGG | - | chr8.1:10732787-10732806 | None:intergenic | 40.0% |
| ! | CCTTTTATCCATTGCTCTCT+TGG | + | chr8.1:10732899-10732918 | MS.gene036665:CDS | 40.0% |
| AAACCAAAAGCCTGCATGGT+TGG | + | chr8.1:10732370-10732389 | MS.gene036665:CDS | 45.0% | |
| AACCGCCAATTCAAAGGATC+AGG | + | chr8.1:10732839-10732858 | MS.gene036665:CDS | 45.0% | |
| AACGACGAACTTGAAGAAGG+CGG | - | chr8.1:10732529-10732548 | None:intergenic | 45.0% | |
| CATTGCTCTCTTGGATGCAA+GGG | + | chr8.1:10732908-10732927 | MS.gene036665:CDS | 45.0% | |
| CCTCAAAACCGCCAATTCAA+AGG | + | chr8.1:10732833-10732852 | MS.gene036665:CDS | 45.0% | |
| CGATGAGATGGAAAGCAATG+AGG | - | chr8.1:10732500-10732519 | None:intergenic | 45.0% | |
| TAAAGAGCTTCCAACCATGC+AGG | - | chr8.1:10732383-10732402 | None:intergenic | 45.0% | |
| TATAGACTGCACCAATGTCC+AGG | + | chr8.1:10732592-10732611 | MS.gene036665:CDS | 45.0% | |
| ! | CCTTTGAATTGGCGGTTTTG+AGG | - | chr8.1:10732836-10732855 | None:intergenic | 45.0% |
| CCATTGCTCTCTTGGATGCA+AGG | + | chr8.1:10732907-10732926 | MS.gene036665:CDS | 50.0% | |
| ! | CTTCCAACCATGCAGGCTTT+TGG | - | chr8.1:10732376-10732395 | None:intergenic | 50.0% |
| GACGAACTTGAAGAAGGCGG+TGG | - | chr8.1:10732526-10732545 | None:intergenic | 55.0% | |
| ! | AGGCGGTGGTAGCGATGAGA+TGG | - | chr8.1:10732512-10732531 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 10732368 | 10732928 | 10732368 | ID=MS.gene036665 |
| chr8.1 | mRNA | 10732368 | 10732928 | 10732368 | ID=MS.gene036665.t1;Parent=MS.gene036665 |
| chr8.1 | exon | 10732368 | 10732613 | 10732368 | ID=MS.gene036665.t1.exon1;Parent=MS.gene036665.t1 |
| chr8.1 | CDS | 10732368 | 10732613 | 10732368 | ID=cds.MS.gene036665.t1;Parent=MS.gene036665.t1 |
| chr8.1 | exon | 10732788 | 10732928 | 10732788 | ID=MS.gene036665.t1.exon2;Parent=MS.gene036665.t1 |
| chr8.1 | CDS | 10732788 | 10732928 | 10732788 | ID=cds.MS.gene036665.t1;Parent=MS.gene036665.t1 |
| Gene Sequence |
| Protein sequence |
