Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036749.t1 | XP_024636802.1 | 64.1 | 117 | 42 | 0 | 1 | 117 | 64 | 180 | 4.90E-27 | 130.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036749.t1 | A0A396IJH9 | 64.1 | 117 | 42 | 0 | 1 | 117 | 64 | 180 | 3.5e-27 | 130.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene036749.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036749.t1 | MTR_3g084720 | 64.000 | 75 | 26 | 1 | 44 | 117 | 106 | 180 | 5.23e-26 | 97.4 |
| MS.gene036749.t1 | MTR_6g465230 | 67.857 | 56 | 18 | 0 | 1 | 56 | 163 | 218 | 1.13e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036749.t1 | AT2G18490 | 59.649 | 57 | 23 | 0 | 58 | 114 | 155 | 211 | 1.66e-20 | 83.6 |
| MS.gene036749.t1 | AT2G18490 | 54.688 | 64 | 29 | 0 | 51 | 114 | 198 | 261 | 2.40e-20 | 84.0 |
| MS.gene036749.t1 | AT5G22990 | 61.905 | 63 | 23 | 1 | 53 | 114 | 227 | 289 | 2.26e-19 | 81.6 |
| MS.gene036749.t1 | AT2G15740 | 56.897 | 58 | 25 | 0 | 57 | 114 | 229 | 286 | 5.37e-19 | 80.5 |
| MS.gene036749.t1 | AT5G42640 | 56.140 | 57 | 25 | 0 | 58 | 114 | 229 | 285 | 8.55e-19 | 79.7 |
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATATTGTTGCTACTAA+TGG | 0.346446 | 8.3:+12901092 | MS.gene036749:CDS |
| CTACTAATGGTGATGAAATC+CGG | 0.385370 | 8.3:+12901105 | MS.gene036749:CDS |
| TTACACTTTGGACACGTATA+CGG | 0.389805 | 8.3:-12901215 | None:intergenic |
| GACGAGCCTCCAAGGAGATC+AGG | 0.460583 | 8.3:-12901038 | None:intergenic |
| GCTAGTACTTTGTTACACTT+TGG | 0.466837 | 8.3:-12901227 | None:intergenic |
| ACAATATTGTTTACCTCAGA+TGG | 0.468933 | 8.3:-12901080 | None:intergenic |
| GCTCATCCTGATCTCCTTGG+AGG | 0.493768 | 8.3:+12901032 | MS.gene036749:CDS |
| TATGCTCATCCTGATCTCCT+TGG | 0.493878 | 8.3:+12901029 | MS.gene036749:CDS |
| AAAGAAGAAATTGTTGATGA+TGG | 0.494936 | 8.3:+12901158 | MS.gene036749:CDS |
| TGTTGATATTATTAGAAGCC+CGG | 0.523272 | 8.3:-12901124 | None:intergenic |
| TACTAATGGTGATGAAATCC+GGG | 0.531216 | 8.3:+12901106 | MS.gene036749:CDS |
| CTAATAATATCAACATGAAG+CGG | 0.561821 | 8.3:+12901132 | MS.gene036749:CDS |
| AAAGTGAAGACGAGCCTCCA+AGG | 0.567472 | 8.3:-12901046 | None:intergenic |
| GAGATCAGGATGAGCATAAG+CGG | 0.608512 | 8.3:-12901024 | None:intergenic |
| TTCACAACAACCGCCATCTG+AGG | 0.636470 | 8.3:+12901067 | MS.gene036749:CDS |
| ATATTGTTTACCTCAGATGG+CGG | 0.654185 | 8.3:-12901077 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAATATTGTTGCTACTAA+TGG | + | chr8.3:12901092-12901111 | MS.gene036749:CDS | 25.0% |
| ! | CTAATAATATCAACATGAAG+CGG | + | chr8.3:12901132-12901151 | MS.gene036749:CDS | 25.0% |
| !! | AAAGAAGAAATTGTTGATGA+TGG | + | chr8.3:12901158-12901177 | MS.gene036749:CDS | 25.0% |
| ACAATATTGTTTACCTCAGA+TGG | - | chr8.3:12901083-12901102 | None:intergenic | 30.0% | |
| AGTCTACCACACAATAAAAA+TGG | + | chr8.3:12901191-12901210 | MS.gene036749:CDS | 30.0% | |
| GAAAAAAGAGATACAAGTCT+AGG | + | chr8.3:12901312-12901331 | MS.gene036749:CDS | 30.0% | |
| TGTTGATATTATTAGAAGCC+CGG | - | chr8.3:12901127-12901146 | None:intergenic | 30.0% | |
| ATATTGTTTACCTCAGATGG+CGG | - | chr8.3:12901080-12901099 | None:intergenic | 35.0% | |
| GCTAGTACTTTGTTACACTT+TGG | - | chr8.3:12901230-12901249 | None:intergenic | 35.0% | |
| TTACACTTTGGACACGTATA+CGG | - | chr8.3:12901218-12901237 | None:intergenic | 35.0% | |
| ! | CTACTAATGGTGATGAAATC+CGG | + | chr8.3:12901105-12901124 | MS.gene036749:CDS | 35.0% |
| ! | TACTAATGGTGATGAAATCC+GGG | + | chr8.3:12901106-12901125 | MS.gene036749:CDS | 35.0% |
| ! | TTTTATTGTGTGGTAGACTG+TGG | - | chr8.3:12901190-12901209 | None:intergenic | 35.0% |
| !! | TACGGTCCATTTTTATTGTG+TGG | - | chr8.3:12901200-12901219 | None:intergenic | 35.0% |
| GAGATCAGGATGAGCATAAG+CGG | - | chr8.3:12901027-12901046 | None:intergenic | 45.0% | |
| TATGCTCATCCTGATCTCCT+TGG | + | chr8.3:12901029-12901048 | MS.gene036749:CDS | 45.0% | |
| AAAGTGAAGACGAGCCTCCA+AGG | - | chr8.3:12901049-12901068 | None:intergenic | 50.0% | |
| TTCACAACAACCGCCATCTG+AGG | + | chr8.3:12901067-12901086 | MS.gene036749:CDS | 50.0% | |
| GCTCATCCTGATCTCCTTGG+AGG | + | chr8.3:12901032-12901051 | MS.gene036749:CDS | 55.0% | |
| GACGAGCCTCCAAGGAGATC+AGG | - | chr8.3:12901041-12901060 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 12901005 | 12901358 | 12901005 | ID=MS.gene036749 |
| chr8.3 | mRNA | 12901005 | 12901358 | 12901005 | ID=MS.gene036749.t1;Parent=MS.gene036749 |
| chr8.3 | exon | 12901005 | 12901358 | 12901005 | ID=MS.gene036749.t1.exon1;Parent=MS.gene036749.t1 |
| chr8.3 | CDS | 12901005 | 12901358 | 12901005 | ID=cds.MS.gene036749.t1;Parent=MS.gene036749.t1 |
| Gene Sequence |
| Protein sequence |
